Transcriptome and Metabolome Analysis Provide New Insights into the Process of Tuberization of <i<Sechium edule</i< Roots

Chayote <i<(Sechium edule)</i< produces edible tubers with high starch content after 1 year of growth but the mechanism of chayote tuberization remains unknown. ‘Tuershao’, a chayote cultivar lacking edible fruits but showing higher tuber yield than traditional chayote cultivars, was use...
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Autor*in:	Lihong Su [verfasserIn] Shaobo Cheng [verfasserIn] Yuhang Liu [verfasserIn] Yongdong Xie [verfasserIn] Zhongqun He [verfasserIn] Mingyue Jia [verfasserIn] Xiaoting Zhou [verfasserIn] Ruijie Zhang [verfasserIn] Chunyan Li [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	gene expression metabolomics tuber yield tuberization transcriptomics

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 23(2022), 12, p 6390
Übergeordnetes Werk:	volume:23 ; year:2022 ; number:12, p 6390

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms23126390

Katalog-ID:	DOAJ044125569

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520			\|a Chayote <i<(Sechium edule)</i< produces edible tubers with high starch content after 1 year of growth but the mechanism of chayote tuberization remains unknown. ‘Tuershao’, a chayote cultivar lacking edible fruits but showing higher tuber yield than traditional chayote cultivars, was used to study tuber formation through integrative analysis of the metabolome and transcriptome profiles at three tuber-growth stages. Starch biosynthesis- and galactose metabolism-related genes and metabolites were significantly upregulated during tuber bulking, whereas genes encoding sugars will eventually be exported transporter (SWEET) and sugar transporter (SUT) were highly expressed during tuber formation. Auxin precursor (indole-3-acetamide) and ethylene precursor, 1-aminocyclopropane-1-carboxylic acid, were upregulated, suggesting that both hormones play pivotal roles in tuber development and maturation. Our data revealed a similar tuber-formation signaling pathway in chayote as in potatoes, including complexes BEL1/KNOX and SP6A/14-3-3/FDL. Down-regulation of the BEL1/KNOX complex and upregulation of 14-3-3 protein implied that these two complexes might have distinct functions in tuber formation. Finally, gene expression and microscopic analysis indicated active cell division during the initial stages of tuber formation. Altogether, the integration of transcriptome and metabolome analyses unraveled an overall molecular network of chayote tuberization that might facilitate its utilization.
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allfields	10.3390/ijms23126390 doi (DE-627)DOAJ044125569 (DE-599)DOAJ85dfb43d9cf945539f16a74e0f9614b9 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Lihong Su verfasserin aut Transcriptome and Metabolome Analysis Provide New Insights into the Process of Tuberization of <i<Sechium edule</i< Roots 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Chayote <i<(Sechium edule)</i< produces edible tubers with high starch content after 1 year of growth but the mechanism of chayote tuberization remains unknown. ‘Tuershao’, a chayote cultivar lacking edible fruits but showing higher tuber yield than traditional chayote cultivars, was used to study tuber formation through integrative analysis of the metabolome and transcriptome profiles at three tuber-growth stages. Starch biosynthesis- and galactose metabolism-related genes and metabolites were significantly upregulated during tuber bulking, whereas genes encoding sugars will eventually be exported transporter (SWEET) and sugar transporter (SUT) were highly expressed during tuber formation. Auxin precursor (indole-3-acetamide) and ethylene precursor, 1-aminocyclopropane-1-carboxylic acid, were upregulated, suggesting that both hormones play pivotal roles in tuber development and maturation. Our data revealed a similar tuber-formation signaling pathway in chayote as in potatoes, including complexes BEL1/KNOX and SP6A/14-3-3/FDL. Down-regulation of the BEL1/KNOX complex and upregulation of 14-3-3 protein implied that these two complexes might have distinct functions in tuber formation. Finally, gene expression and microscopic analysis indicated active cell division during the initial stages of tuber formation. Altogether, the integration of transcriptome and metabolome analyses unraveled an overall molecular network of chayote tuberization that might facilitate its utilization. gene expression <i<Sechium edule</i< metabolomics tuber yield tuberization transcriptomics Biology (General) Chemistry Shaobo Cheng verfasserin aut Yuhang Liu verfasserin aut Yongdong Xie verfasserin aut Zhongqun He verfasserin aut Mingyue Jia verfasserin aut Xiaoting Zhou verfasserin aut Ruijie Zhang verfasserin aut Chunyan Li verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 12, p 6390 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:12, p 6390 https://doi.org/10.3390/ijms23126390 kostenfrei https://doaj.org/article/85dfb43d9cf945539f16a74e0f9614b9 kostenfrei https://www.mdpi.com/1422-0067/23/12/6390 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 12, p 6390
spelling	10.3390/ijms23126390 doi (DE-627)DOAJ044125569 (DE-599)DOAJ85dfb43d9cf945539f16a74e0f9614b9 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Lihong Su verfasserin aut Transcriptome and Metabolome Analysis Provide New Insights into the Process of Tuberization of <i<Sechium edule</i< Roots 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Chayote <i<(Sechium edule)</i< produces edible tubers with high starch content after 1 year of growth but the mechanism of chayote tuberization remains unknown. ‘Tuershao’, a chayote cultivar lacking edible fruits but showing higher tuber yield than traditional chayote cultivars, was used to study tuber formation through integrative analysis of the metabolome and transcriptome profiles at three tuber-growth stages. Starch biosynthesis- and galactose metabolism-related genes and metabolites were significantly upregulated during tuber bulking, whereas genes encoding sugars will eventually be exported transporter (SWEET) and sugar transporter (SUT) were highly expressed during tuber formation. Auxin precursor (indole-3-acetamide) and ethylene precursor, 1-aminocyclopropane-1-carboxylic acid, were upregulated, suggesting that both hormones play pivotal roles in tuber development and maturation. Our data revealed a similar tuber-formation signaling pathway in chayote as in potatoes, including complexes BEL1/KNOX and SP6A/14-3-3/FDL. Down-regulation of the BEL1/KNOX complex and upregulation of 14-3-3 protein implied that these two complexes might have distinct functions in tuber formation. Finally, gene expression and microscopic analysis indicated active cell division during the initial stages of tuber formation. Altogether, the integration of transcriptome and metabolome analyses unraveled an overall molecular network of chayote tuberization that might facilitate its utilization. gene expression <i<Sechium edule</i< metabolomics tuber yield tuberization transcriptomics Biology (General) Chemistry Shaobo Cheng verfasserin aut Yuhang Liu verfasserin aut Yongdong Xie verfasserin aut Zhongqun He verfasserin aut Mingyue Jia verfasserin aut Xiaoting Zhou verfasserin aut Ruijie Zhang verfasserin aut Chunyan Li verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 12, p 6390 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:12, p 6390 https://doi.org/10.3390/ijms23126390 kostenfrei https://doaj.org/article/85dfb43d9cf945539f16a74e0f9614b9 kostenfrei https://www.mdpi.com/1422-0067/23/12/6390 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 12, p 6390
allfields_unstemmed	10.3390/ijms23126390 doi (DE-627)DOAJ044125569 (DE-599)DOAJ85dfb43d9cf945539f16a74e0f9614b9 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Lihong Su verfasserin aut Transcriptome and Metabolome Analysis Provide New Insights into the Process of Tuberization of <i<Sechium edule</i< Roots 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Chayote <i<(Sechium edule)</i< produces edible tubers with high starch content after 1 year of growth but the mechanism of chayote tuberization remains unknown. ‘Tuershao’, a chayote cultivar lacking edible fruits but showing higher tuber yield than traditional chayote cultivars, was used to study tuber formation through integrative analysis of the metabolome and transcriptome profiles at three tuber-growth stages. Starch biosynthesis- and galactose metabolism-related genes and metabolites were significantly upregulated during tuber bulking, whereas genes encoding sugars will eventually be exported transporter (SWEET) and sugar transporter (SUT) were highly expressed during tuber formation. Auxin precursor (indole-3-acetamide) and ethylene precursor, 1-aminocyclopropane-1-carboxylic acid, were upregulated, suggesting that both hormones play pivotal roles in tuber development and maturation. Our data revealed a similar tuber-formation signaling pathway in chayote as in potatoes, including complexes BEL1/KNOX and SP6A/14-3-3/FDL. Down-regulation of the BEL1/KNOX complex and upregulation of 14-3-3 protein implied that these two complexes might have distinct functions in tuber formation. Finally, gene expression and microscopic analysis indicated active cell division during the initial stages of tuber formation. Altogether, the integration of transcriptome and metabolome analyses unraveled an overall molecular network of chayote tuberization that might facilitate its utilization. gene expression <i<Sechium edule</i< metabolomics tuber yield tuberization transcriptomics Biology (General) Chemistry Shaobo Cheng verfasserin aut Yuhang Liu verfasserin aut Yongdong Xie verfasserin aut Zhongqun He verfasserin aut Mingyue Jia verfasserin aut Xiaoting Zhou verfasserin aut Ruijie Zhang verfasserin aut Chunyan Li verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 12, p 6390 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:12, p 6390 https://doi.org/10.3390/ijms23126390 kostenfrei https://doaj.org/article/85dfb43d9cf945539f16a74e0f9614b9 kostenfrei https://www.mdpi.com/1422-0067/23/12/6390 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 12, p 6390
allfieldsGer	10.3390/ijms23126390 doi (DE-627)DOAJ044125569 (DE-599)DOAJ85dfb43d9cf945539f16a74e0f9614b9 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Lihong Su verfasserin aut Transcriptome and Metabolome Analysis Provide New Insights into the Process of Tuberization of <i<Sechium edule</i< Roots 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Chayote <i<(Sechium edule)</i< produces edible tubers with high starch content after 1 year of growth but the mechanism of chayote tuberization remains unknown. ‘Tuershao’, a chayote cultivar lacking edible fruits but showing higher tuber yield than traditional chayote cultivars, was used to study tuber formation through integrative analysis of the metabolome and transcriptome profiles at three tuber-growth stages. Starch biosynthesis- and galactose metabolism-related genes and metabolites were significantly upregulated during tuber bulking, whereas genes encoding sugars will eventually be exported transporter (SWEET) and sugar transporter (SUT) were highly expressed during tuber formation. Auxin precursor (indole-3-acetamide) and ethylene precursor, 1-aminocyclopropane-1-carboxylic acid, were upregulated, suggesting that both hormones play pivotal roles in tuber development and maturation. Our data revealed a similar tuber-formation signaling pathway in chayote as in potatoes, including complexes BEL1/KNOX and SP6A/14-3-3/FDL. Down-regulation of the BEL1/KNOX complex and upregulation of 14-3-3 protein implied that these two complexes might have distinct functions in tuber formation. Finally, gene expression and microscopic analysis indicated active cell division during the initial stages of tuber formation. Altogether, the integration of transcriptome and metabolome analyses unraveled an overall molecular network of chayote tuberization that might facilitate its utilization. gene expression <i<Sechium edule</i< metabolomics tuber yield tuberization transcriptomics Biology (General) Chemistry Shaobo Cheng verfasserin aut Yuhang Liu verfasserin aut Yongdong Xie verfasserin aut Zhongqun He verfasserin aut Mingyue Jia verfasserin aut Xiaoting Zhou verfasserin aut Ruijie Zhang verfasserin aut Chunyan Li verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 12, p 6390 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:12, p 6390 https://doi.org/10.3390/ijms23126390 kostenfrei https://doaj.org/article/85dfb43d9cf945539f16a74e0f9614b9 kostenfrei https://www.mdpi.com/1422-0067/23/12/6390 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 12, p 6390
allfieldsSound	10.3390/ijms23126390 doi (DE-627)DOAJ044125569 (DE-599)DOAJ85dfb43d9cf945539f16a74e0f9614b9 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Lihong Su verfasserin aut Transcriptome and Metabolome Analysis Provide New Insights into the Process of Tuberization of <i<Sechium edule</i< Roots 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Chayote <i<(Sechium edule)</i< produces edible tubers with high starch content after 1 year of growth but the mechanism of chayote tuberization remains unknown. ‘Tuershao’, a chayote cultivar lacking edible fruits but showing higher tuber yield than traditional chayote cultivars, was used to study tuber formation through integrative analysis of the metabolome and transcriptome profiles at three tuber-growth stages. Starch biosynthesis- and galactose metabolism-related genes and metabolites were significantly upregulated during tuber bulking, whereas genes encoding sugars will eventually be exported transporter (SWEET) and sugar transporter (SUT) were highly expressed during tuber formation. Auxin precursor (indole-3-acetamide) and ethylene precursor, 1-aminocyclopropane-1-carboxylic acid, were upregulated, suggesting that both hormones play pivotal roles in tuber development and maturation. Our data revealed a similar tuber-formation signaling pathway in chayote as in potatoes, including complexes BEL1/KNOX and SP6A/14-3-3/FDL. Down-regulation of the BEL1/KNOX complex and upregulation of 14-3-3 protein implied that these two complexes might have distinct functions in tuber formation. Finally, gene expression and microscopic analysis indicated active cell division during the initial stages of tuber formation. Altogether, the integration of transcriptome and metabolome analyses unraveled an overall molecular network of chayote tuberization that might facilitate its utilization. gene expression <i<Sechium edule</i< metabolomics tuber yield tuberization transcriptomics Biology (General) Chemistry Shaobo Cheng verfasserin aut Yuhang Liu verfasserin aut Yongdong Xie verfasserin aut Zhongqun He verfasserin aut Mingyue Jia verfasserin aut Xiaoting Zhou verfasserin aut Ruijie Zhang verfasserin aut Chunyan Li verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 23(2022), 12, p 6390 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:23 year:2022 number:12, p 6390 https://doi.org/10.3390/ijms23126390 kostenfrei https://doaj.org/article/85dfb43d9cf945539f16a74e0f9614b9 kostenfrei https://www.mdpi.com/1422-0067/23/12/6390 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2022 12, p 6390
language	English
source	In International Journal of Molecular Sciences 23(2022), 12, p 6390 volume:23 year:2022 number:12, p 6390
sourceStr	In International Journal of Molecular Sciences 23(2022), 12, p 6390 volume:23 year:2022 number:12, p 6390
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	gene expression <i<Sechium edule</i< metabolomics tuber yield tuberization transcriptomics Biology (General) Chemistry
isfreeaccess_bool	true
container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Lihong Su @@aut@@ Shaobo Cheng @@aut@@ Yuhang Liu @@aut@@ Yongdong Xie @@aut@@ Zhongqun He @@aut@@ Mingyue Jia @@aut@@ Xiaoting Zhou @@aut@@ Ruijie Zhang @@aut@@ Chunyan Li @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	316340715
id	DOAJ044125569
language_de	englisch
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callnumber-first	Q - Science
author	Lihong Su
spellingShingle	Lihong Su misc QH301-705.5 misc QD1-999 misc gene expression misc <i<Sechium edule</i< misc metabolomics misc tuber yield misc tuberization misc transcriptomics misc Biology (General) misc Chemistry Transcriptome and Metabolome Analysis Provide New Insights into the Process of Tuberization of <i<Sechium edule</i< Roots
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topic_title	QH301-705.5 QD1-999 Transcriptome and Metabolome Analysis Provide New Insights into the Process of Tuberization of <i<Sechium edule</i< Roots gene expression <i<Sechium edule</i< metabolomics tuber yield tuberization transcriptomics
topic	misc QH301-705.5 misc QD1-999 misc gene expression misc <i<Sechium edule</i< misc metabolomics misc tuber yield misc tuberization misc transcriptomics misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc gene expression misc <i<Sechium edule</i< misc metabolomics misc tuber yield misc tuberization misc transcriptomics misc Biology (General) misc Chemistry
topic_browse	misc QH301-705.5 misc QD1-999 misc gene expression misc <i<Sechium edule</i< misc metabolomics misc tuber yield misc tuberization misc transcriptomics misc Biology (General) misc Chemistry
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title	Transcriptome and Metabolome Analysis Provide New Insights into the Process of Tuberization of <i<Sechium edule</i< Roots
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title_full	Transcriptome and Metabolome Analysis Provide New Insights into the Process of Tuberization of <i<Sechium edule</i< Roots
author_sort	Lihong Su
journal	International Journal of Molecular Sciences
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author_browse	Lihong Su Shaobo Cheng Yuhang Liu Yongdong Xie Zhongqun He Mingyue Jia Xiaoting Zhou Ruijie Zhang Chunyan Li
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author-letter	Lihong Su
doi_str_mv	10.3390/ijms23126390
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title_sort	transcriptome and metabolome analysis provide new insights into the process of tuberization of <i<sechium edule</i< roots
callnumber	QH301-705.5
title_auth	Transcriptome and Metabolome Analysis Provide New Insights into the Process of Tuberization of <i<Sechium edule</i< Roots
abstract	Chayote <i<(Sechium edule)</i< produces edible tubers with high starch content after 1 year of growth but the mechanism of chayote tuberization remains unknown. ‘Tuershao’, a chayote cultivar lacking edible fruits but showing higher tuber yield than traditional chayote cultivars, was used to study tuber formation through integrative analysis of the metabolome and transcriptome profiles at three tuber-growth stages. Starch biosynthesis- and galactose metabolism-related genes and metabolites were significantly upregulated during tuber bulking, whereas genes encoding sugars will eventually be exported transporter (SWEET) and sugar transporter (SUT) were highly expressed during tuber formation. Auxin precursor (indole-3-acetamide) and ethylene precursor, 1-aminocyclopropane-1-carboxylic acid, were upregulated, suggesting that both hormones play pivotal roles in tuber development and maturation. Our data revealed a similar tuber-formation signaling pathway in chayote as in potatoes, including complexes BEL1/KNOX and SP6A/14-3-3/FDL. Down-regulation of the BEL1/KNOX complex and upregulation of 14-3-3 protein implied that these two complexes might have distinct functions in tuber formation. Finally, gene expression and microscopic analysis indicated active cell division during the initial stages of tuber formation. Altogether, the integration of transcriptome and metabolome analyses unraveled an overall molecular network of chayote tuberization that might facilitate its utilization.
abstractGer	Chayote <i<(Sechium edule)</i< produces edible tubers with high starch content after 1 year of growth but the mechanism of chayote tuberization remains unknown. ‘Tuershao’, a chayote cultivar lacking edible fruits but showing higher tuber yield than traditional chayote cultivars, was used to study tuber formation through integrative analysis of the metabolome and transcriptome profiles at three tuber-growth stages. Starch biosynthesis- and galactose metabolism-related genes and metabolites were significantly upregulated during tuber bulking, whereas genes encoding sugars will eventually be exported transporter (SWEET) and sugar transporter (SUT) were highly expressed during tuber formation. Auxin precursor (indole-3-acetamide) and ethylene precursor, 1-aminocyclopropane-1-carboxylic acid, were upregulated, suggesting that both hormones play pivotal roles in tuber development and maturation. Our data revealed a similar tuber-formation signaling pathway in chayote as in potatoes, including complexes BEL1/KNOX and SP6A/14-3-3/FDL. Down-regulation of the BEL1/KNOX complex and upregulation of 14-3-3 protein implied that these two complexes might have distinct functions in tuber formation. Finally, gene expression and microscopic analysis indicated active cell division during the initial stages of tuber formation. Altogether, the integration of transcriptome and metabolome analyses unraveled an overall molecular network of chayote tuberization that might facilitate its utilization.
abstract_unstemmed	Chayote <i<(Sechium edule)</i< produces edible tubers with high starch content after 1 year of growth but the mechanism of chayote tuberization remains unknown. ‘Tuershao’, a chayote cultivar lacking edible fruits but showing higher tuber yield than traditional chayote cultivars, was used to study tuber formation through integrative analysis of the metabolome and transcriptome profiles at three tuber-growth stages. Starch biosynthesis- and galactose metabolism-related genes and metabolites were significantly upregulated during tuber bulking, whereas genes encoding sugars will eventually be exported transporter (SWEET) and sugar transporter (SUT) were highly expressed during tuber formation. Auxin precursor (indole-3-acetamide) and ethylene precursor, 1-aminocyclopropane-1-carboxylic acid, were upregulated, suggesting that both hormones play pivotal roles in tuber development and maturation. Our data revealed a similar tuber-formation signaling pathway in chayote as in potatoes, including complexes BEL1/KNOX and SP6A/14-3-3/FDL. Down-regulation of the BEL1/KNOX complex and upregulation of 14-3-3 protein implied that these two complexes might have distinct functions in tuber formation. Finally, gene expression and microscopic analysis indicated active cell division during the initial stages of tuber formation. Altogether, the integration of transcriptome and metabolome analyses unraveled an overall molecular network of chayote tuberization that might facilitate its utilization.
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container_issue	12, p 6390
title_short	Transcriptome and Metabolome Analysis Provide New Insights into the Process of Tuberization of <i<Sechium edule</i< Roots
url	https://doi.org/10.3390/ijms23126390 https://doaj.org/article/85dfb43d9cf945539f16a74e0f9614b9 https://www.mdpi.com/1422-0067/23/12/6390 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067
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