Single-Cell Transcriptomic Analysis of the Mouse Pancreas: Characteristic Features of Pancreatic Ductal Cells in Chronic Pancreatitis

Chronic pancreatitis (CP) is a fibroinflammatory disorder of the pancreas. Our understanding of CP pathogenesis is partly limited by the incomplete characterization of pancreatic cell types. Here, we performed single-cell RNA sequencing on 3825 cells from the pancreas of one control mouse and mice w...
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Autor*in:	Xiaotong Mao [verfasserIn] Shenghan Mao [verfasserIn] Lei Wang [verfasserIn] Hui Jiang [verfasserIn] Shunjiang Deng [verfasserIn] Yuanchen Wang [verfasserIn] Jun Ye [verfasserIn] Zhaoshen Li [verfasserIn] Wenbin Zou [verfasserIn] Zhuan Liao [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	chronic pancreatitis single-cell analysis transcriptome mouse model caerulein mesenchymal cells

Übergeordnetes Werk:	In: Genes - MDPI AG, 2010, 13(2022), 6, p 1015
Übergeordnetes Werk:	volume:13 ; year:2022 ; number:6, p 1015

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/genes13061015

Katalog-ID:	DOAJ044161425

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520			\|a Chronic pancreatitis (CP) is a fibroinflammatory disorder of the pancreas. Our understanding of CP pathogenesis is partly limited by the incomplete characterization of pancreatic cell types. Here, we performed single-cell RNA sequencing on 3825 cells from the pancreas of one control mouse and mice with caerulein-induced CP. An analysis of the single-cell transcriptomes revealed 16 unique clusters and cell type-specific gene expression patterns in the mouse pancreas. Sub-clustering of the pancreatic mesenchymal cells from the control mouse revealed four clusters of cells with specific gene expression profiles (combinatorial expressions of <i<Smoc2</i<, <i<Cxcl14</i<, <i<Tnfaip6</i<, and <i<Fn1</i<). We observed that immune cells in the pancreas of the CP mice were abundant and diverse in cellular type. Compared to the control, 547 upregulated genes (including <i<Mmp7</i<, <i<Ttr</i<, <i<Rgs5</i<, <i<Adh1</i<, and <i<Cldn2</i<) and 257 downregulated genes were identified in ductal cells from the CP group. The elevated expression levels of MMP7 and TTR were further verified in the pancreatic ducts of CP patients. This study provides a preliminary description of the single-cell transcriptome profiles of mouse pancreata and accurately demonstrates the characteristics of pancreatic ductal cells in CP. The findings provide insight into novel disease-specific biomarkers and potential therapeutic targets of CP.
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allfields	10.3390/genes13061015 doi (DE-627)DOAJ044161425 (DE-599)DOAJd646446c2fdb44afbe901405c356f3f4 DE-627 ger DE-627 rakwb eng QH426-470 Xiaotong Mao verfasserin aut Single-Cell Transcriptomic Analysis of the Mouse Pancreas: Characteristic Features of Pancreatic Ductal Cells in Chronic Pancreatitis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Chronic pancreatitis (CP) is a fibroinflammatory disorder of the pancreas. Our understanding of CP pathogenesis is partly limited by the incomplete characterization of pancreatic cell types. Here, we performed single-cell RNA sequencing on 3825 cells from the pancreas of one control mouse and mice with caerulein-induced CP. An analysis of the single-cell transcriptomes revealed 16 unique clusters and cell type-specific gene expression patterns in the mouse pancreas. Sub-clustering of the pancreatic mesenchymal cells from the control mouse revealed four clusters of cells with specific gene expression profiles (combinatorial expressions of <i<Smoc2</i<, <i<Cxcl14</i<, <i<Tnfaip6</i<, and <i<Fn1</i<). We observed that immune cells in the pancreas of the CP mice were abundant and diverse in cellular type. Compared to the control, 547 upregulated genes (including <i<Mmp7</i<, <i<Ttr</i<, <i<Rgs5</i<, <i<Adh1</i<, and <i<Cldn2</i<) and 257 downregulated genes were identified in ductal cells from the CP group. The elevated expression levels of MMP7 and TTR were further verified in the pancreatic ducts of CP patients. This study provides a preliminary description of the single-cell transcriptome profiles of mouse pancreata and accurately demonstrates the characteristics of pancreatic ductal cells in CP. The findings provide insight into novel disease-specific biomarkers and potential therapeutic targets of CP. chronic pancreatitis single-cell analysis transcriptome mouse model caerulein mesenchymal cells Genetics Shenghan Mao verfasserin aut Lei Wang verfasserin aut Hui Jiang verfasserin aut Shunjiang Deng verfasserin aut Yuanchen Wang verfasserin aut Jun Ye verfasserin aut Zhaoshen Li verfasserin aut Wenbin Zou verfasserin aut Zhuan Liao verfasserin aut In Genes MDPI AG, 2010 13(2022), 6, p 1015 (DE-627)614096537 (DE-600)2527218-4 20734425 nnns volume:13 year:2022 number:6, p 1015 https://doi.org/10.3390/genes13061015 kostenfrei https://doaj.org/article/d646446c2fdb44afbe901405c356f3f4 kostenfrei https://www.mdpi.com/2073-4425/13/6/1015 kostenfrei https://doaj.org/toc/2073-4425 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 6, p 1015
spelling	10.3390/genes13061015 doi (DE-627)DOAJ044161425 (DE-599)DOAJd646446c2fdb44afbe901405c356f3f4 DE-627 ger DE-627 rakwb eng QH426-470 Xiaotong Mao verfasserin aut Single-Cell Transcriptomic Analysis of the Mouse Pancreas: Characteristic Features of Pancreatic Ductal Cells in Chronic Pancreatitis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Chronic pancreatitis (CP) is a fibroinflammatory disorder of the pancreas. Our understanding of CP pathogenesis is partly limited by the incomplete characterization of pancreatic cell types. Here, we performed single-cell RNA sequencing on 3825 cells from the pancreas of one control mouse and mice with caerulein-induced CP. An analysis of the single-cell transcriptomes revealed 16 unique clusters and cell type-specific gene expression patterns in the mouse pancreas. Sub-clustering of the pancreatic mesenchymal cells from the control mouse revealed four clusters of cells with specific gene expression profiles (combinatorial expressions of <i<Smoc2</i<, <i<Cxcl14</i<, <i<Tnfaip6</i<, and <i<Fn1</i<). We observed that immune cells in the pancreas of the CP mice were abundant and diverse in cellular type. Compared to the control, 547 upregulated genes (including <i<Mmp7</i<, <i<Ttr</i<, <i<Rgs5</i<, <i<Adh1</i<, and <i<Cldn2</i<) and 257 downregulated genes were identified in ductal cells from the CP group. The elevated expression levels of MMP7 and TTR were further verified in the pancreatic ducts of CP patients. This study provides a preliminary description of the single-cell transcriptome profiles of mouse pancreata and accurately demonstrates the characteristics of pancreatic ductal cells in CP. The findings provide insight into novel disease-specific biomarkers and potential therapeutic targets of CP. chronic pancreatitis single-cell analysis transcriptome mouse model caerulein mesenchymal cells Genetics Shenghan Mao verfasserin aut Lei Wang verfasserin aut Hui Jiang verfasserin aut Shunjiang Deng verfasserin aut Yuanchen Wang verfasserin aut Jun Ye verfasserin aut Zhaoshen Li verfasserin aut Wenbin Zou verfasserin aut Zhuan Liao verfasserin aut In Genes MDPI AG, 2010 13(2022), 6, p 1015 (DE-627)614096537 (DE-600)2527218-4 20734425 nnns volume:13 year:2022 number:6, p 1015 https://doi.org/10.3390/genes13061015 kostenfrei https://doaj.org/article/d646446c2fdb44afbe901405c356f3f4 kostenfrei https://www.mdpi.com/2073-4425/13/6/1015 kostenfrei https://doaj.org/toc/2073-4425 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 6, p 1015
allfields_unstemmed	10.3390/genes13061015 doi (DE-627)DOAJ044161425 (DE-599)DOAJd646446c2fdb44afbe901405c356f3f4 DE-627 ger DE-627 rakwb eng QH426-470 Xiaotong Mao verfasserin aut Single-Cell Transcriptomic Analysis of the Mouse Pancreas: Characteristic Features of Pancreatic Ductal Cells in Chronic Pancreatitis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Chronic pancreatitis (CP) is a fibroinflammatory disorder of the pancreas. Our understanding of CP pathogenesis is partly limited by the incomplete characterization of pancreatic cell types. Here, we performed single-cell RNA sequencing on 3825 cells from the pancreas of one control mouse and mice with caerulein-induced CP. An analysis of the single-cell transcriptomes revealed 16 unique clusters and cell type-specific gene expression patterns in the mouse pancreas. Sub-clustering of the pancreatic mesenchymal cells from the control mouse revealed four clusters of cells with specific gene expression profiles (combinatorial expressions of <i<Smoc2</i<, <i<Cxcl14</i<, <i<Tnfaip6</i<, and <i<Fn1</i<). We observed that immune cells in the pancreas of the CP mice were abundant and diverse in cellular type. Compared to the control, 547 upregulated genes (including <i<Mmp7</i<, <i<Ttr</i<, <i<Rgs5</i<, <i<Adh1</i<, and <i<Cldn2</i<) and 257 downregulated genes were identified in ductal cells from the CP group. The elevated expression levels of MMP7 and TTR were further verified in the pancreatic ducts of CP patients. This study provides a preliminary description of the single-cell transcriptome profiles of mouse pancreata and accurately demonstrates the characteristics of pancreatic ductal cells in CP. The findings provide insight into novel disease-specific biomarkers and potential therapeutic targets of CP. chronic pancreatitis single-cell analysis transcriptome mouse model caerulein mesenchymal cells Genetics Shenghan Mao verfasserin aut Lei Wang verfasserin aut Hui Jiang verfasserin aut Shunjiang Deng verfasserin aut Yuanchen Wang verfasserin aut Jun Ye verfasserin aut Zhaoshen Li verfasserin aut Wenbin Zou verfasserin aut Zhuan Liao verfasserin aut In Genes MDPI AG, 2010 13(2022), 6, p 1015 (DE-627)614096537 (DE-600)2527218-4 20734425 nnns volume:13 year:2022 number:6, p 1015 https://doi.org/10.3390/genes13061015 kostenfrei https://doaj.org/article/d646446c2fdb44afbe901405c356f3f4 kostenfrei https://www.mdpi.com/2073-4425/13/6/1015 kostenfrei https://doaj.org/toc/2073-4425 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 6, p 1015
allfieldsGer	10.3390/genes13061015 doi (DE-627)DOAJ044161425 (DE-599)DOAJd646446c2fdb44afbe901405c356f3f4 DE-627 ger DE-627 rakwb eng QH426-470 Xiaotong Mao verfasserin aut Single-Cell Transcriptomic Analysis of the Mouse Pancreas: Characteristic Features of Pancreatic Ductal Cells in Chronic Pancreatitis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Chronic pancreatitis (CP) is a fibroinflammatory disorder of the pancreas. Our understanding of CP pathogenesis is partly limited by the incomplete characterization of pancreatic cell types. Here, we performed single-cell RNA sequencing on 3825 cells from the pancreas of one control mouse and mice with caerulein-induced CP. An analysis of the single-cell transcriptomes revealed 16 unique clusters and cell type-specific gene expression patterns in the mouse pancreas. Sub-clustering of the pancreatic mesenchymal cells from the control mouse revealed four clusters of cells with specific gene expression profiles (combinatorial expressions of <i<Smoc2</i<, <i<Cxcl14</i<, <i<Tnfaip6</i<, and <i<Fn1</i<). We observed that immune cells in the pancreas of the CP mice were abundant and diverse in cellular type. Compared to the control, 547 upregulated genes (including <i<Mmp7</i<, <i<Ttr</i<, <i<Rgs5</i<, <i<Adh1</i<, and <i<Cldn2</i<) and 257 downregulated genes were identified in ductal cells from the CP group. The elevated expression levels of MMP7 and TTR were further verified in the pancreatic ducts of CP patients. This study provides a preliminary description of the single-cell transcriptome profiles of mouse pancreata and accurately demonstrates the characteristics of pancreatic ductal cells in CP. The findings provide insight into novel disease-specific biomarkers and potential therapeutic targets of CP. chronic pancreatitis single-cell analysis transcriptome mouse model caerulein mesenchymal cells Genetics Shenghan Mao verfasserin aut Lei Wang verfasserin aut Hui Jiang verfasserin aut Shunjiang Deng verfasserin aut Yuanchen Wang verfasserin aut Jun Ye verfasserin aut Zhaoshen Li verfasserin aut Wenbin Zou verfasserin aut Zhuan Liao verfasserin aut In Genes MDPI AG, 2010 13(2022), 6, p 1015 (DE-627)614096537 (DE-600)2527218-4 20734425 nnns volume:13 year:2022 number:6, p 1015 https://doi.org/10.3390/genes13061015 kostenfrei https://doaj.org/article/d646446c2fdb44afbe901405c356f3f4 kostenfrei https://www.mdpi.com/2073-4425/13/6/1015 kostenfrei https://doaj.org/toc/2073-4425 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 6, p 1015
allfieldsSound	10.3390/genes13061015 doi (DE-627)DOAJ044161425 (DE-599)DOAJd646446c2fdb44afbe901405c356f3f4 DE-627 ger DE-627 rakwb eng QH426-470 Xiaotong Mao verfasserin aut Single-Cell Transcriptomic Analysis of the Mouse Pancreas: Characteristic Features of Pancreatic Ductal Cells in Chronic Pancreatitis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Chronic pancreatitis (CP) is a fibroinflammatory disorder of the pancreas. Our understanding of CP pathogenesis is partly limited by the incomplete characterization of pancreatic cell types. Here, we performed single-cell RNA sequencing on 3825 cells from the pancreas of one control mouse and mice with caerulein-induced CP. An analysis of the single-cell transcriptomes revealed 16 unique clusters and cell type-specific gene expression patterns in the mouse pancreas. Sub-clustering of the pancreatic mesenchymal cells from the control mouse revealed four clusters of cells with specific gene expression profiles (combinatorial expressions of <i<Smoc2</i<, <i<Cxcl14</i<, <i<Tnfaip6</i<, and <i<Fn1</i<). We observed that immune cells in the pancreas of the CP mice were abundant and diverse in cellular type. Compared to the control, 547 upregulated genes (including <i<Mmp7</i<, <i<Ttr</i<, <i<Rgs5</i<, <i<Adh1</i<, and <i<Cldn2</i<) and 257 downregulated genes were identified in ductal cells from the CP group. The elevated expression levels of MMP7 and TTR were further verified in the pancreatic ducts of CP patients. This study provides a preliminary description of the single-cell transcriptome profiles of mouse pancreata and accurately demonstrates the characteristics of pancreatic ductal cells in CP. The findings provide insight into novel disease-specific biomarkers and potential therapeutic targets of CP. chronic pancreatitis single-cell analysis transcriptome mouse model caerulein mesenchymal cells Genetics Shenghan Mao verfasserin aut Lei Wang verfasserin aut Hui Jiang verfasserin aut Shunjiang Deng verfasserin aut Yuanchen Wang verfasserin aut Jun Ye verfasserin aut Zhaoshen Li verfasserin aut Wenbin Zou verfasserin aut Zhuan Liao verfasserin aut In Genes MDPI AG, 2010 13(2022), 6, p 1015 (DE-627)614096537 (DE-600)2527218-4 20734425 nnns volume:13 year:2022 number:6, p 1015 https://doi.org/10.3390/genes13061015 kostenfrei https://doaj.org/article/d646446c2fdb44afbe901405c356f3f4 kostenfrei https://www.mdpi.com/2073-4425/13/6/1015 kostenfrei https://doaj.org/toc/2073-4425 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 13 2022 6, p 1015
language	English
source	In Genes 13(2022), 6, p 1015 volume:13 year:2022 number:6, p 1015
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authorswithroles_txt_mv	Xiaotong Mao @@aut@@ Shenghan Mao @@aut@@ Lei Wang @@aut@@ Hui Jiang @@aut@@ Shunjiang Deng @@aut@@ Yuanchen Wang @@aut@@ Jun Ye @@aut@@ Zhaoshen Li @@aut@@ Wenbin Zou @@aut@@ Zhuan Liao @@aut@@
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callnumber-first	Q - Science
author	Xiaotong Mao
spellingShingle	Xiaotong Mao misc QH426-470 misc chronic pancreatitis misc single-cell analysis misc transcriptome misc mouse model misc caerulein misc mesenchymal cells misc Genetics Single-Cell Transcriptomic Analysis of the Mouse Pancreas: Characteristic Features of Pancreatic Ductal Cells in Chronic Pancreatitis
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topic_title	QH426-470 Single-Cell Transcriptomic Analysis of the Mouse Pancreas: Characteristic Features of Pancreatic Ductal Cells in Chronic Pancreatitis chronic pancreatitis single-cell analysis transcriptome mouse model caerulein mesenchymal cells
topic	misc QH426-470 misc chronic pancreatitis misc single-cell analysis misc transcriptome misc mouse model misc caerulein misc mesenchymal cells misc Genetics
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title	Single-Cell Transcriptomic Analysis of the Mouse Pancreas: Characteristic Features of Pancreatic Ductal Cells in Chronic Pancreatitis
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title_full	Single-Cell Transcriptomic Analysis of the Mouse Pancreas: Characteristic Features of Pancreatic Ductal Cells in Chronic Pancreatitis
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title_sort	single-cell transcriptomic analysis of the mouse pancreas: characteristic features of pancreatic ductal cells in chronic pancreatitis
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title_auth	Single-Cell Transcriptomic Analysis of the Mouse Pancreas: Characteristic Features of Pancreatic Ductal Cells in Chronic Pancreatitis
abstract	Chronic pancreatitis (CP) is a fibroinflammatory disorder of the pancreas. Our understanding of CP pathogenesis is partly limited by the incomplete characterization of pancreatic cell types. Here, we performed single-cell RNA sequencing on 3825 cells from the pancreas of one control mouse and mice with caerulein-induced CP. An analysis of the single-cell transcriptomes revealed 16 unique clusters and cell type-specific gene expression patterns in the mouse pancreas. Sub-clustering of the pancreatic mesenchymal cells from the control mouse revealed four clusters of cells with specific gene expression profiles (combinatorial expressions of <i<Smoc2</i<, <i<Cxcl14</i<, <i<Tnfaip6</i<, and <i<Fn1</i<). We observed that immune cells in the pancreas of the CP mice were abundant and diverse in cellular type. Compared to the control, 547 upregulated genes (including <i<Mmp7</i<, <i<Ttr</i<, <i<Rgs5</i<, <i<Adh1</i<, and <i<Cldn2</i<) and 257 downregulated genes were identified in ductal cells from the CP group. The elevated expression levels of MMP7 and TTR were further verified in the pancreatic ducts of CP patients. This study provides a preliminary description of the single-cell transcriptome profiles of mouse pancreata and accurately demonstrates the characteristics of pancreatic ductal cells in CP. The findings provide insight into novel disease-specific biomarkers and potential therapeutic targets of CP.
abstractGer	Chronic pancreatitis (CP) is a fibroinflammatory disorder of the pancreas. Our understanding of CP pathogenesis is partly limited by the incomplete characterization of pancreatic cell types. Here, we performed single-cell RNA sequencing on 3825 cells from the pancreas of one control mouse and mice with caerulein-induced CP. An analysis of the single-cell transcriptomes revealed 16 unique clusters and cell type-specific gene expression patterns in the mouse pancreas. Sub-clustering of the pancreatic mesenchymal cells from the control mouse revealed four clusters of cells with specific gene expression profiles (combinatorial expressions of <i<Smoc2</i<, <i<Cxcl14</i<, <i<Tnfaip6</i<, and <i<Fn1</i<). We observed that immune cells in the pancreas of the CP mice were abundant and diverse in cellular type. Compared to the control, 547 upregulated genes (including <i<Mmp7</i<, <i<Ttr</i<, <i<Rgs5</i<, <i<Adh1</i<, and <i<Cldn2</i<) and 257 downregulated genes were identified in ductal cells from the CP group. The elevated expression levels of MMP7 and TTR were further verified in the pancreatic ducts of CP patients. This study provides a preliminary description of the single-cell transcriptome profiles of mouse pancreata and accurately demonstrates the characteristics of pancreatic ductal cells in CP. The findings provide insight into novel disease-specific biomarkers and potential therapeutic targets of CP.
abstract_unstemmed	Chronic pancreatitis (CP) is a fibroinflammatory disorder of the pancreas. Our understanding of CP pathogenesis is partly limited by the incomplete characterization of pancreatic cell types. Here, we performed single-cell RNA sequencing on 3825 cells from the pancreas of one control mouse and mice with caerulein-induced CP. An analysis of the single-cell transcriptomes revealed 16 unique clusters and cell type-specific gene expression patterns in the mouse pancreas. Sub-clustering of the pancreatic mesenchymal cells from the control mouse revealed four clusters of cells with specific gene expression profiles (combinatorial expressions of <i<Smoc2</i<, <i<Cxcl14</i<, <i<Tnfaip6</i<, and <i<Fn1</i<). We observed that immune cells in the pancreas of the CP mice were abundant and diverse in cellular type. Compared to the control, 547 upregulated genes (including <i<Mmp7</i<, <i<Ttr</i<, <i<Rgs5</i<, <i<Adh1</i<, and <i<Cldn2</i<) and 257 downregulated genes were identified in ductal cells from the CP group. The elevated expression levels of MMP7 and TTR were further verified in the pancreatic ducts of CP patients. This study provides a preliminary description of the single-cell transcriptome profiles of mouse pancreata and accurately demonstrates the characteristics of pancreatic ductal cells in CP. The findings provide insight into novel disease-specific biomarkers and potential therapeutic targets of CP.
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title_short	Single-Cell Transcriptomic Analysis of the Mouse Pancreas: Characteristic Features of Pancreatic Ductal Cells in Chronic Pancreatitis
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up_date	2024-07-03T21:33:14.223Z
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The elevated expression levels of MMP7 and TTR were further verified in the pancreatic ducts of CP patients. This study provides a preliminary description of the single-cell transcriptome profiles of mouse pancreata and accurately demonstrates the characteristics of pancreatic ductal cells in CP. 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Single-Cell Transcriptomic Analysis of the Mouse Pancreas: Characteristic Features of Pancreatic Ductal Cells in Chronic Pancreatitis

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