Exogenous classic phytohormones have limited regulatory effects on fructan and primary carbohydrate metabolism in perennial ryegrass (Lolium perenne L.)

Fructans are polymers of fructose and one of the main constituents of water-soluble carbohydrates in forage grasses and cereal crops of temperate climates. Fructans are involved in cold and drought resistance, regrowth following defoliation and early spring growth, seed filling, have beneficial effects on human health and are used for industrial processes. Perennial ryegrass (Lolium perenne L.) serves as model species to study fructan metabolism. Fructan metabolism is under the control of both synthesis by fructosyltransferases (FTs) and breakdown through fructan exohydrolases (FEHs). The accumulation of fructans can be triggered by high sucrose levels and abiotic stress conditions such as drought and cold stress. However, detailed studies on the mechanisms involved in the regulation of fructan metabolism are scarce. Since different phytohormones, especially abscisic acid (ABA), are known to play an important role in abiotic stress responses, the possible short term regulation of the enzymes involved in fructan metabolism by the five classical phytohormones was investigated. Therefore, the activities of enzymes involved in fructan synthesis and breakdown, the expression levels for the corresponding genes and levels for water-soluble carbohydrates were determined following pulse treatments with ABA, auxin (AUX), ethylene (ET), gibberellic acid (GA) or kinetin (KIN). The most pronounced fast effects were a transient increase of FT activities by AUX, KIN, ABA and ET, while minor effects were evident for 1-FEH activity with an increased activity in response to KIN and a decrease by GA. Fructan and sucrose levels were not affected. This observed discrepancy demonstrates the importance of determining enzyme activities to obtain insight into the physiological traits and ultimately the plant phenotype. The comparative analyses of activities for seven key enzymes of primary carbohydrate metabolism revealed no co-regulation between enzymes of the fructan and sucrose pool. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Anna eGasperl [verfasserIn] Annette eMorvan-Bertrand [verfasserIn] Marie-Pascale ePrud'Homme [verfasserIn] Eric eVan Der Graaff [verfasserIn] Thomas eRoitsch [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	phytohormones Enzymatic activity Perennial ryegrass Fructosyltransferase Fructan metabolism Fructan exohydrolase

Übergeordnetes Werk:	In: Frontiers in Plant Science - Frontiers Media S.A., 2011, 6(2016)
Übergeordnetes Werk:	volume:6 ; year:2016

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fpls.2015.01251

Katalog-ID:	DOAJ044263023

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allfields	10.3389/fpls.2015.01251 doi (DE-627)DOAJ044263023 (DE-599)DOAJ673bf61b808948c0a7afda4c422fa342 DE-627 ger DE-627 rakwb eng SB1-1110 Anna eGasperl verfasserin aut Exogenous classic phytohormones have limited regulatory effects on fructan and primary carbohydrate metabolism in perennial ryegrass (Lolium perenne L.) 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Fructans are polymers of fructose and one of the main constituents of water-soluble carbohydrates in forage grasses and cereal crops of temperate climates. Fructans are involved in cold and drought resistance, regrowth following defoliation and early spring growth, seed filling, have beneficial effects on human health and are used for industrial processes. Perennial ryegrass (Lolium perenne L.) serves as model species to study fructan metabolism. Fructan metabolism is under the control of both synthesis by fructosyltransferases (FTs) and breakdown through fructan exohydrolases (FEHs). The accumulation of fructans can be triggered by high sucrose levels and abiotic stress conditions such as drought and cold stress. However, detailed studies on the mechanisms involved in the regulation of fructan metabolism are scarce. Since different phytohormones, especially abscisic acid (ABA), are known to play an important role in abiotic stress responses, the possible short term regulation of the enzymes involved in fructan metabolism by the five classical phytohormones was investigated. Therefore, the activities of enzymes involved in fructan synthesis and breakdown, the expression levels for the corresponding genes and levels for water-soluble carbohydrates were determined following pulse treatments with ABA, auxin (AUX), ethylene (ET), gibberellic acid (GA) or kinetin (KIN). The most pronounced fast effects were a transient increase of FT activities by AUX, KIN, ABA and ET, while minor effects were evident for 1-FEH activity with an increased activity in response to KIN and a decrease by GA. Fructan and sucrose levels were not affected. This observed discrepancy demonstrates the importance of determining enzyme activities to obtain insight into the physiological traits and ultimately the plant phenotype. The comparative analyses of activities for seven key enzymes of primary carbohydrate metabolism revealed no co-regulation between enzymes of the fructan and sucrose pool. phytohormones Enzymatic activity Perennial ryegrass Fructosyltransferase Fructan metabolism Fructan exohydrolase Plant culture Annette eMorvan-Bertrand verfasserin aut Annette eMorvan-Bertrand verfasserin aut Annette eMorvan-Bertrand verfasserin aut Marie-Pascale ePrud'Homme verfasserin aut Marie-Pascale ePrud'Homme verfasserin aut Marie-Pascale ePrud'Homme verfasserin aut Eric eVan Der Graaff verfasserin aut Eric eVan Der Graaff verfasserin aut Thomas eRoitsch verfasserin aut Thomas eRoitsch verfasserin aut Thomas eRoitsch verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 6(2016) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:6 year:2016 https://doi.org/10.3389/fpls.2015.01251 kostenfrei https://doaj.org/article/673bf61b808948c0a7afda4c422fa342 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fpls.2015.01251/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2016
spelling	10.3389/fpls.2015.01251 doi (DE-627)DOAJ044263023 (DE-599)DOAJ673bf61b808948c0a7afda4c422fa342 DE-627 ger DE-627 rakwb eng SB1-1110 Anna eGasperl verfasserin aut Exogenous classic phytohormones have limited regulatory effects on fructan and primary carbohydrate metabolism in perennial ryegrass (Lolium perenne L.) 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Fructans are polymers of fructose and one of the main constituents of water-soluble carbohydrates in forage grasses and cereal crops of temperate climates. Fructans are involved in cold and drought resistance, regrowth following defoliation and early spring growth, seed filling, have beneficial effects on human health and are used for industrial processes. Perennial ryegrass (Lolium perenne L.) serves as model species to study fructan metabolism. Fructan metabolism is under the control of both synthesis by fructosyltransferases (FTs) and breakdown through fructan exohydrolases (FEHs). The accumulation of fructans can be triggered by high sucrose levels and abiotic stress conditions such as drought and cold stress. However, detailed studies on the mechanisms involved in the regulation of fructan metabolism are scarce. Since different phytohormones, especially abscisic acid (ABA), are known to play an important role in abiotic stress responses, the possible short term regulation of the enzymes involved in fructan metabolism by the five classical phytohormones was investigated. Therefore, the activities of enzymes involved in fructan synthesis and breakdown, the expression levels for the corresponding genes and levels for water-soluble carbohydrates were determined following pulse treatments with ABA, auxin (AUX), ethylene (ET), gibberellic acid (GA) or kinetin (KIN). The most pronounced fast effects were a transient increase of FT activities by AUX, KIN, ABA and ET, while minor effects were evident for 1-FEH activity with an increased activity in response to KIN and a decrease by GA. Fructan and sucrose levels were not affected. This observed discrepancy demonstrates the importance of determining enzyme activities to obtain insight into the physiological traits and ultimately the plant phenotype. The comparative analyses of activities for seven key enzymes of primary carbohydrate metabolism revealed no co-regulation between enzymes of the fructan and sucrose pool. phytohormones Enzymatic activity Perennial ryegrass Fructosyltransferase Fructan metabolism Fructan exohydrolase Plant culture Annette eMorvan-Bertrand verfasserin aut Annette eMorvan-Bertrand verfasserin aut Annette eMorvan-Bertrand verfasserin aut Marie-Pascale ePrud'Homme verfasserin aut Marie-Pascale ePrud'Homme verfasserin aut Marie-Pascale ePrud'Homme verfasserin aut Eric eVan Der Graaff verfasserin aut Eric eVan Der Graaff verfasserin aut Thomas eRoitsch verfasserin aut Thomas eRoitsch verfasserin aut Thomas eRoitsch verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 6(2016) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:6 year:2016 https://doi.org/10.3389/fpls.2015.01251 kostenfrei https://doaj.org/article/673bf61b808948c0a7afda4c422fa342 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fpls.2015.01251/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2016
allfields_unstemmed	10.3389/fpls.2015.01251 doi (DE-627)DOAJ044263023 (DE-599)DOAJ673bf61b808948c0a7afda4c422fa342 DE-627 ger DE-627 rakwb eng SB1-1110 Anna eGasperl verfasserin aut Exogenous classic phytohormones have limited regulatory effects on fructan and primary carbohydrate metabolism in perennial ryegrass (Lolium perenne L.) 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Fructans are polymers of fructose and one of the main constituents of water-soluble carbohydrates in forage grasses and cereal crops of temperate climates. Fructans are involved in cold and drought resistance, regrowth following defoliation and early spring growth, seed filling, have beneficial effects on human health and are used for industrial processes. Perennial ryegrass (Lolium perenne L.) serves as model species to study fructan metabolism. Fructan metabolism is under the control of both synthesis by fructosyltransferases (FTs) and breakdown through fructan exohydrolases (FEHs). The accumulation of fructans can be triggered by high sucrose levels and abiotic stress conditions such as drought and cold stress. However, detailed studies on the mechanisms involved in the regulation of fructan metabolism are scarce. Since different phytohormones, especially abscisic acid (ABA), are known to play an important role in abiotic stress responses, the possible short term regulation of the enzymes involved in fructan metabolism by the five classical phytohormones was investigated. Therefore, the activities of enzymes involved in fructan synthesis and breakdown, the expression levels for the corresponding genes and levels for water-soluble carbohydrates were determined following pulse treatments with ABA, auxin (AUX), ethylene (ET), gibberellic acid (GA) or kinetin (KIN). The most pronounced fast effects were a transient increase of FT activities by AUX, KIN, ABA and ET, while minor effects were evident for 1-FEH activity with an increased activity in response to KIN and a decrease by GA. Fructan and sucrose levels were not affected. This observed discrepancy demonstrates the importance of determining enzyme activities to obtain insight into the physiological traits and ultimately the plant phenotype. The comparative analyses of activities for seven key enzymes of primary carbohydrate metabolism revealed no co-regulation between enzymes of the fructan and sucrose pool. phytohormones Enzymatic activity Perennial ryegrass Fructosyltransferase Fructan metabolism Fructan exohydrolase Plant culture Annette eMorvan-Bertrand verfasserin aut Annette eMorvan-Bertrand verfasserin aut Annette eMorvan-Bertrand verfasserin aut Marie-Pascale ePrud'Homme verfasserin aut Marie-Pascale ePrud'Homme verfasserin aut Marie-Pascale ePrud'Homme verfasserin aut Eric eVan Der Graaff verfasserin aut Eric eVan Der Graaff verfasserin aut Thomas eRoitsch verfasserin aut Thomas eRoitsch verfasserin aut Thomas eRoitsch verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 6(2016) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:6 year:2016 https://doi.org/10.3389/fpls.2015.01251 kostenfrei https://doaj.org/article/673bf61b808948c0a7afda4c422fa342 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fpls.2015.01251/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2016
allfieldsGer	10.3389/fpls.2015.01251 doi (DE-627)DOAJ044263023 (DE-599)DOAJ673bf61b808948c0a7afda4c422fa342 DE-627 ger DE-627 rakwb eng SB1-1110 Anna eGasperl verfasserin aut Exogenous classic phytohormones have limited regulatory effects on fructan and primary carbohydrate metabolism in perennial ryegrass (Lolium perenne L.) 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Fructans are polymers of fructose and one of the main constituents of water-soluble carbohydrates in forage grasses and cereal crops of temperate climates. Fructans are involved in cold and drought resistance, regrowth following defoliation and early spring growth, seed filling, have beneficial effects on human health and are used for industrial processes. Perennial ryegrass (Lolium perenne L.) serves as model species to study fructan metabolism. Fructan metabolism is under the control of both synthesis by fructosyltransferases (FTs) and breakdown through fructan exohydrolases (FEHs). The accumulation of fructans can be triggered by high sucrose levels and abiotic stress conditions such as drought and cold stress. However, detailed studies on the mechanisms involved in the regulation of fructan metabolism are scarce. Since different phytohormones, especially abscisic acid (ABA), are known to play an important role in abiotic stress responses, the possible short term regulation of the enzymes involved in fructan metabolism by the five classical phytohormones was investigated. Therefore, the activities of enzymes involved in fructan synthesis and breakdown, the expression levels for the corresponding genes and levels for water-soluble carbohydrates were determined following pulse treatments with ABA, auxin (AUX), ethylene (ET), gibberellic acid (GA) or kinetin (KIN). The most pronounced fast effects were a transient increase of FT activities by AUX, KIN, ABA and ET, while minor effects were evident for 1-FEH activity with an increased activity in response to KIN and a decrease by GA. Fructan and sucrose levels were not affected. This observed discrepancy demonstrates the importance of determining enzyme activities to obtain insight into the physiological traits and ultimately the plant phenotype. The comparative analyses of activities for seven key enzymes of primary carbohydrate metabolism revealed no co-regulation between enzymes of the fructan and sucrose pool. phytohormones Enzymatic activity Perennial ryegrass Fructosyltransferase Fructan metabolism Fructan exohydrolase Plant culture Annette eMorvan-Bertrand verfasserin aut Annette eMorvan-Bertrand verfasserin aut Annette eMorvan-Bertrand verfasserin aut Marie-Pascale ePrud'Homme verfasserin aut Marie-Pascale ePrud'Homme verfasserin aut Marie-Pascale ePrud'Homme verfasserin aut Eric eVan Der Graaff verfasserin aut Eric eVan Der Graaff verfasserin aut Thomas eRoitsch verfasserin aut Thomas eRoitsch verfasserin aut Thomas eRoitsch verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 6(2016) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:6 year:2016 https://doi.org/10.3389/fpls.2015.01251 kostenfrei https://doaj.org/article/673bf61b808948c0a7afda4c422fa342 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fpls.2015.01251/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2016
allfieldsSound	10.3389/fpls.2015.01251 doi (DE-627)DOAJ044263023 (DE-599)DOAJ673bf61b808948c0a7afda4c422fa342 DE-627 ger DE-627 rakwb eng SB1-1110 Anna eGasperl verfasserin aut Exogenous classic phytohormones have limited regulatory effects on fructan and primary carbohydrate metabolism in perennial ryegrass (Lolium perenne L.) 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Fructans are polymers of fructose and one of the main constituents of water-soluble carbohydrates in forage grasses and cereal crops of temperate climates. Fructans are involved in cold and drought resistance, regrowth following defoliation and early spring growth, seed filling, have beneficial effects on human health and are used for industrial processes. Perennial ryegrass (Lolium perenne L.) serves as model species to study fructan metabolism. Fructan metabolism is under the control of both synthesis by fructosyltransferases (FTs) and breakdown through fructan exohydrolases (FEHs). The accumulation of fructans can be triggered by high sucrose levels and abiotic stress conditions such as drought and cold stress. However, detailed studies on the mechanisms involved in the regulation of fructan metabolism are scarce. Since different phytohormones, especially abscisic acid (ABA), are known to play an important role in abiotic stress responses, the possible short term regulation of the enzymes involved in fructan metabolism by the five classical phytohormones was investigated. Therefore, the activities of enzymes involved in fructan synthesis and breakdown, the expression levels for the corresponding genes and levels for water-soluble carbohydrates were determined following pulse treatments with ABA, auxin (AUX), ethylene (ET), gibberellic acid (GA) or kinetin (KIN). The most pronounced fast effects were a transient increase of FT activities by AUX, KIN, ABA and ET, while minor effects were evident for 1-FEH activity with an increased activity in response to KIN and a decrease by GA. Fructan and sucrose levels were not affected. This observed discrepancy demonstrates the importance of determining enzyme activities to obtain insight into the physiological traits and ultimately the plant phenotype. The comparative analyses of activities for seven key enzymes of primary carbohydrate metabolism revealed no co-regulation between enzymes of the fructan and sucrose pool. phytohormones Enzymatic activity Perennial ryegrass Fructosyltransferase Fructan metabolism Fructan exohydrolase Plant culture Annette eMorvan-Bertrand verfasserin aut Annette eMorvan-Bertrand verfasserin aut Annette eMorvan-Bertrand verfasserin aut Marie-Pascale ePrud'Homme verfasserin aut Marie-Pascale ePrud'Homme verfasserin aut Marie-Pascale ePrud'Homme verfasserin aut Eric eVan Der Graaff verfasserin aut Eric eVan Der Graaff verfasserin aut Thomas eRoitsch verfasserin aut Thomas eRoitsch verfasserin aut Thomas eRoitsch verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 6(2016) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:6 year:2016 https://doi.org/10.3389/fpls.2015.01251 kostenfrei https://doaj.org/article/673bf61b808948c0a7afda4c422fa342 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fpls.2015.01251/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2016
language	English
source	In Frontiers in Plant Science 6(2016) volume:6 year:2016
sourceStr	In Frontiers in Plant Science 6(2016) volume:6 year:2016
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	phytohormones Enzymatic activity Perennial ryegrass Fructosyltransferase Fructan metabolism Fructan exohydrolase Plant culture
isfreeaccess_bool	true
container_title	Frontiers in Plant Science
authorswithroles_txt_mv	Anna eGasperl @@aut@@ Annette eMorvan-Bertrand @@aut@@ Marie-Pascale ePrud'Homme @@aut@@ Eric eVan Der Graaff @@aut@@ Thomas eRoitsch @@aut@@
publishDateDaySort_date	2016-01-01T00:00:00Z
hierarchy_top_id	662359240
id	DOAJ044263023
language_de	englisch
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callnumber-first	S - Agriculture
author	Anna eGasperl
spellingShingle	Anna eGasperl misc SB1-1110 misc phytohormones misc Enzymatic activity misc Perennial ryegrass misc Fructosyltransferase misc Fructan metabolism misc Fructan exohydrolase misc Plant culture Exogenous classic phytohormones have limited regulatory effects on fructan and primary carbohydrate metabolism in perennial ryegrass (Lolium perenne L.)
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topic_title	SB1-1110 Exogenous classic phytohormones have limited regulatory effects on fructan and primary carbohydrate metabolism in perennial ryegrass (Lolium perenne L.) phytohormones Enzymatic activity Perennial ryegrass Fructosyltransferase Fructan metabolism Fructan exohydrolase
topic	misc SB1-1110 misc phytohormones misc Enzymatic activity misc Perennial ryegrass misc Fructosyltransferase misc Fructan metabolism misc Fructan exohydrolase misc Plant culture
topic_unstemmed	misc SB1-1110 misc phytohormones misc Enzymatic activity misc Perennial ryegrass misc Fructosyltransferase misc Fructan metabolism misc Fructan exohydrolase misc Plant culture
topic_browse	misc SB1-1110 misc phytohormones misc Enzymatic activity misc Perennial ryegrass misc Fructosyltransferase misc Fructan metabolism misc Fructan exohydrolase misc Plant culture
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title	Exogenous classic phytohormones have limited regulatory effects on fructan and primary carbohydrate metabolism in perennial ryegrass (Lolium perenne L.)
ctrlnum	(DE-627)DOAJ044263023 (DE-599)DOAJ673bf61b808948c0a7afda4c422fa342
title_full	Exogenous classic phytohormones have limited regulatory effects on fructan and primary carbohydrate metabolism in perennial ryegrass (Lolium perenne L.)
author_sort	Anna eGasperl
journal	Frontiers in Plant Science
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author_browse	Anna eGasperl Annette eMorvan-Bertrand Marie-Pascale ePrud'Homme Eric eVan Der Graaff Thomas eRoitsch
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title_sort	exogenous classic phytohormones have limited regulatory effects on fructan and primary carbohydrate metabolism in perennial ryegrass (lolium perenne l.)
callnumber	SB1-1110
title_auth	Exogenous classic phytohormones have limited regulatory effects on fructan and primary carbohydrate metabolism in perennial ryegrass (Lolium perenne L.)
abstract	Fructans are polymers of fructose and one of the main constituents of water-soluble carbohydrates in forage grasses and cereal crops of temperate climates. Fructans are involved in cold and drought resistance, regrowth following defoliation and early spring growth, seed filling, have beneficial effects on human health and are used for industrial processes. Perennial ryegrass (Lolium perenne L.) serves as model species to study fructan metabolism. Fructan metabolism is under the control of both synthesis by fructosyltransferases (FTs) and breakdown through fructan exohydrolases (FEHs). The accumulation of fructans can be triggered by high sucrose levels and abiotic stress conditions such as drought and cold stress. However, detailed studies on the mechanisms involved in the regulation of fructan metabolism are scarce. Since different phytohormones, especially abscisic acid (ABA), are known to play an important role in abiotic stress responses, the possible short term regulation of the enzymes involved in fructan metabolism by the five classical phytohormones was investigated. Therefore, the activities of enzymes involved in fructan synthesis and breakdown, the expression levels for the corresponding genes and levels for water-soluble carbohydrates were determined following pulse treatments with ABA, auxin (AUX), ethylene (ET), gibberellic acid (GA) or kinetin (KIN). The most pronounced fast effects were a transient increase of FT activities by AUX, KIN, ABA and ET, while minor effects were evident for 1-FEH activity with an increased activity in response to KIN and a decrease by GA. Fructan and sucrose levels were not affected. This observed discrepancy demonstrates the importance of determining enzyme activities to obtain insight into the physiological traits and ultimately the plant phenotype. The comparative analyses of activities for seven key enzymes of primary carbohydrate metabolism revealed no co-regulation between enzymes of the fructan and sucrose pool.
abstractGer	Fructans are polymers of fructose and one of the main constituents of water-soluble carbohydrates in forage grasses and cereal crops of temperate climates. Fructans are involved in cold and drought resistance, regrowth following defoliation and early spring growth, seed filling, have beneficial effects on human health and are used for industrial processes. Perennial ryegrass (Lolium perenne L.) serves as model species to study fructan metabolism. Fructan metabolism is under the control of both synthesis by fructosyltransferases (FTs) and breakdown through fructan exohydrolases (FEHs). The accumulation of fructans can be triggered by high sucrose levels and abiotic stress conditions such as drought and cold stress. However, detailed studies on the mechanisms involved in the regulation of fructan metabolism are scarce. Since different phytohormones, especially abscisic acid (ABA), are known to play an important role in abiotic stress responses, the possible short term regulation of the enzymes involved in fructan metabolism by the five classical phytohormones was investigated. Therefore, the activities of enzymes involved in fructan synthesis and breakdown, the expression levels for the corresponding genes and levels for water-soluble carbohydrates were determined following pulse treatments with ABA, auxin (AUX), ethylene (ET), gibberellic acid (GA) or kinetin (KIN). The most pronounced fast effects were a transient increase of FT activities by AUX, KIN, ABA and ET, while minor effects were evident for 1-FEH activity with an increased activity in response to KIN and a decrease by GA. Fructan and sucrose levels were not affected. This observed discrepancy demonstrates the importance of determining enzyme activities to obtain insight into the physiological traits and ultimately the plant phenotype. The comparative analyses of activities for seven key enzymes of primary carbohydrate metabolism revealed no co-regulation between enzymes of the fructan and sucrose pool.
abstract_unstemmed	Fructans are polymers of fructose and one of the main constituents of water-soluble carbohydrates in forage grasses and cereal crops of temperate climates. Fructans are involved in cold and drought resistance, regrowth following defoliation and early spring growth, seed filling, have beneficial effects on human health and are used for industrial processes. Perennial ryegrass (Lolium perenne L.) serves as model species to study fructan metabolism. Fructan metabolism is under the control of both synthesis by fructosyltransferases (FTs) and breakdown through fructan exohydrolases (FEHs). The accumulation of fructans can be triggered by high sucrose levels and abiotic stress conditions such as drought and cold stress. However, detailed studies on the mechanisms involved in the regulation of fructan metabolism are scarce. Since different phytohormones, especially abscisic acid (ABA), are known to play an important role in abiotic stress responses, the possible short term regulation of the enzymes involved in fructan metabolism by the five classical phytohormones was investigated. Therefore, the activities of enzymes involved in fructan synthesis and breakdown, the expression levels for the corresponding genes and levels for water-soluble carbohydrates were determined following pulse treatments with ABA, auxin (AUX), ethylene (ET), gibberellic acid (GA) or kinetin (KIN). The most pronounced fast effects were a transient increase of FT activities by AUX, KIN, ABA and ET, while minor effects were evident for 1-FEH activity with an increased activity in response to KIN and a decrease by GA. Fructan and sucrose levels were not affected. This observed discrepancy demonstrates the importance of determining enzyme activities to obtain insight into the physiological traits and ultimately the plant phenotype. The comparative analyses of activities for seven key enzymes of primary carbohydrate metabolism revealed no co-regulation between enzymes of the fructan and sucrose pool.
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title_short	Exogenous classic phytohormones have limited regulatory effects on fructan and primary carbohydrate metabolism in perennial ryegrass (Lolium perenne L.)
url	https://doi.org/10.3389/fpls.2015.01251 https://doaj.org/article/673bf61b808948c0a7afda4c422fa342 http://journal.frontiersin.org/Journal/10.3389/fpls.2015.01251/full https://doaj.org/toc/1664-462X
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Exogenous classic phytohormones have limited regulatory effects on fructan and primary carbohydrate metabolism in perennial ryegrass (Lolium perenne L.)

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