A simple high‐throughput protocol for the extraction and quantification of inorganic phosphate in rice leaves

Premise Phosphorus (P) is an essential macronutrient that is often limited in agricultural systems. Determining inorganic phosphate (Pi) contents of plant tissues is crucial for evaluating plant P status. Here, we present a simple, high‐throughput colorimetric microplate technique to measure Pi cont...
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Autor*in:	Sompop Pinit [verfasserIn] Supachitra Chadchawan [verfasserIn] Juthamas Chaiwanon [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	inorganic phosphate microplate molybdenum blue assay rice

Übergeordnetes Werk:	In: Applications in Plant Sciences - Wiley, 2013, 8(2020), 10, Seite n/a-n/a
Übergeordnetes Werk:	volume:8 ; year:2020 ; number:10 ; pages:n/a-n/a

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1002/aps3.11395

Katalog-ID:	DOAJ044710305

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520			\|a Premise Phosphorus (P) is an essential macronutrient that is often limited in agricultural systems. Determining inorganic phosphate (Pi) contents of plant tissues is crucial for evaluating plant P status. Here, we present a simple, high‐throughput colorimetric microplate technique to measure Pi contents in rice (Oryza sativa) leaf tissues, based on the molybdenum blue reaction. Methods and Results We used a hole puncher to sample small equal areas of leaf tissue for Pi extraction. We removed the leaf grinding and weighing steps, which are time‐consuming and normally required to release Pi from the tissues and to measure the biomass for data normalization, respectively. We showed that the punching method yielded comparable results to the conventional grinding method for two rice cultivars grown under various levels of P supply. Conclusions Compared with existing techniques, this protocol is more suited to an initial screening, enabling one researcher to determine the Pi contents of thousands of rice leaf samples within a few hours.
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allfields	10.1002/aps3.11395 doi (DE-627)DOAJ044710305 (DE-599)DOAJ3f5f81ce46c849aeadf417e31f4b8d5a DE-627 ger DE-627 rakwb eng QH301-705.5 QK1-989 Sompop Pinit verfasserin aut A simple high‐throughput protocol for the extraction and quantification of inorganic phosphate in rice leaves 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Premise Phosphorus (P) is an essential macronutrient that is often limited in agricultural systems. Determining inorganic phosphate (Pi) contents of plant tissues is crucial for evaluating plant P status. Here, we present a simple, high‐throughput colorimetric microplate technique to measure Pi contents in rice (Oryza sativa) leaf tissues, based on the molybdenum blue reaction. Methods and Results We used a hole puncher to sample small equal areas of leaf tissue for Pi extraction. We removed the leaf grinding and weighing steps, which are time‐consuming and normally required to release Pi from the tissues and to measure the biomass for data normalization, respectively. We showed that the punching method yielded comparable results to the conventional grinding method for two rice cultivars grown under various levels of P supply. Conclusions Compared with existing techniques, this protocol is more suited to an initial screening, enabling one researcher to determine the Pi contents of thousands of rice leaf samples within a few hours. inorganic phosphate microplate molybdenum blue assay rice Biology (General) Botany Supachitra Chadchawan verfasserin aut Juthamas Chaiwanon verfasserin aut In Applications in Plant Sciences Wiley, 2013 8(2020), 10, Seite n/a-n/a (DE-627)735133506 (DE-600)2699923-7 21680450 nnns volume:8 year:2020 number:10 pages:n/a-n/a https://doi.org/10.1002/aps3.11395 kostenfrei https://doaj.org/article/3f5f81ce46c849aeadf417e31f4b8d5a kostenfrei https://doi.org/10.1002/aps3.11395 kostenfrei https://doaj.org/toc/2168-0450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2020 10 n/a-n/a
spelling	10.1002/aps3.11395 doi (DE-627)DOAJ044710305 (DE-599)DOAJ3f5f81ce46c849aeadf417e31f4b8d5a DE-627 ger DE-627 rakwb eng QH301-705.5 QK1-989 Sompop Pinit verfasserin aut A simple high‐throughput protocol for the extraction and quantification of inorganic phosphate in rice leaves 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Premise Phosphorus (P) is an essential macronutrient that is often limited in agricultural systems. Determining inorganic phosphate (Pi) contents of plant tissues is crucial for evaluating plant P status. Here, we present a simple, high‐throughput colorimetric microplate technique to measure Pi contents in rice (Oryza sativa) leaf tissues, based on the molybdenum blue reaction. Methods and Results We used a hole puncher to sample small equal areas of leaf tissue for Pi extraction. We removed the leaf grinding and weighing steps, which are time‐consuming and normally required to release Pi from the tissues and to measure the biomass for data normalization, respectively. We showed that the punching method yielded comparable results to the conventional grinding method for two rice cultivars grown under various levels of P supply. Conclusions Compared with existing techniques, this protocol is more suited to an initial screening, enabling one researcher to determine the Pi contents of thousands of rice leaf samples within a few hours. inorganic phosphate microplate molybdenum blue assay rice Biology (General) Botany Supachitra Chadchawan verfasserin aut Juthamas Chaiwanon verfasserin aut In Applications in Plant Sciences Wiley, 2013 8(2020), 10, Seite n/a-n/a (DE-627)735133506 (DE-600)2699923-7 21680450 nnns volume:8 year:2020 number:10 pages:n/a-n/a https://doi.org/10.1002/aps3.11395 kostenfrei https://doaj.org/article/3f5f81ce46c849aeadf417e31f4b8d5a kostenfrei https://doi.org/10.1002/aps3.11395 kostenfrei https://doaj.org/toc/2168-0450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2020 10 n/a-n/a
allfields_unstemmed	10.1002/aps3.11395 doi (DE-627)DOAJ044710305 (DE-599)DOAJ3f5f81ce46c849aeadf417e31f4b8d5a DE-627 ger DE-627 rakwb eng QH301-705.5 QK1-989 Sompop Pinit verfasserin aut A simple high‐throughput protocol for the extraction and quantification of inorganic phosphate in rice leaves 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Premise Phosphorus (P) is an essential macronutrient that is often limited in agricultural systems. Determining inorganic phosphate (Pi) contents of plant tissues is crucial for evaluating plant P status. Here, we present a simple, high‐throughput colorimetric microplate technique to measure Pi contents in rice (Oryza sativa) leaf tissues, based on the molybdenum blue reaction. Methods and Results We used a hole puncher to sample small equal areas of leaf tissue for Pi extraction. We removed the leaf grinding and weighing steps, which are time‐consuming and normally required to release Pi from the tissues and to measure the biomass for data normalization, respectively. We showed that the punching method yielded comparable results to the conventional grinding method for two rice cultivars grown under various levels of P supply. Conclusions Compared with existing techniques, this protocol is more suited to an initial screening, enabling one researcher to determine the Pi contents of thousands of rice leaf samples within a few hours. inorganic phosphate microplate molybdenum blue assay rice Biology (General) Botany Supachitra Chadchawan verfasserin aut Juthamas Chaiwanon verfasserin aut In Applications in Plant Sciences Wiley, 2013 8(2020), 10, Seite n/a-n/a (DE-627)735133506 (DE-600)2699923-7 21680450 nnns volume:8 year:2020 number:10 pages:n/a-n/a https://doi.org/10.1002/aps3.11395 kostenfrei https://doaj.org/article/3f5f81ce46c849aeadf417e31f4b8d5a kostenfrei https://doi.org/10.1002/aps3.11395 kostenfrei https://doaj.org/toc/2168-0450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2020 10 n/a-n/a
allfieldsGer	10.1002/aps3.11395 doi (DE-627)DOAJ044710305 (DE-599)DOAJ3f5f81ce46c849aeadf417e31f4b8d5a DE-627 ger DE-627 rakwb eng QH301-705.5 QK1-989 Sompop Pinit verfasserin aut A simple high‐throughput protocol for the extraction and quantification of inorganic phosphate in rice leaves 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Premise Phosphorus (P) is an essential macronutrient that is often limited in agricultural systems. Determining inorganic phosphate (Pi) contents of plant tissues is crucial for evaluating plant P status. Here, we present a simple, high‐throughput colorimetric microplate technique to measure Pi contents in rice (Oryza sativa) leaf tissues, based on the molybdenum blue reaction. Methods and Results We used a hole puncher to sample small equal areas of leaf tissue for Pi extraction. We removed the leaf grinding and weighing steps, which are time‐consuming and normally required to release Pi from the tissues and to measure the biomass for data normalization, respectively. We showed that the punching method yielded comparable results to the conventional grinding method for two rice cultivars grown under various levels of P supply. Conclusions Compared with existing techniques, this protocol is more suited to an initial screening, enabling one researcher to determine the Pi contents of thousands of rice leaf samples within a few hours. inorganic phosphate microplate molybdenum blue assay rice Biology (General) Botany Supachitra Chadchawan verfasserin aut Juthamas Chaiwanon verfasserin aut In Applications in Plant Sciences Wiley, 2013 8(2020), 10, Seite n/a-n/a (DE-627)735133506 (DE-600)2699923-7 21680450 nnns volume:8 year:2020 number:10 pages:n/a-n/a https://doi.org/10.1002/aps3.11395 kostenfrei https://doaj.org/article/3f5f81ce46c849aeadf417e31f4b8d5a kostenfrei https://doi.org/10.1002/aps3.11395 kostenfrei https://doaj.org/toc/2168-0450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2020 10 n/a-n/a
allfieldsSound	10.1002/aps3.11395 doi (DE-627)DOAJ044710305 (DE-599)DOAJ3f5f81ce46c849aeadf417e31f4b8d5a DE-627 ger DE-627 rakwb eng QH301-705.5 QK1-989 Sompop Pinit verfasserin aut A simple high‐throughput protocol for the extraction and quantification of inorganic phosphate in rice leaves 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Premise Phosphorus (P) is an essential macronutrient that is often limited in agricultural systems. Determining inorganic phosphate (Pi) contents of plant tissues is crucial for evaluating plant P status. Here, we present a simple, high‐throughput colorimetric microplate technique to measure Pi contents in rice (Oryza sativa) leaf tissues, based on the molybdenum blue reaction. Methods and Results We used a hole puncher to sample small equal areas of leaf tissue for Pi extraction. We removed the leaf grinding and weighing steps, which are time‐consuming and normally required to release Pi from the tissues and to measure the biomass for data normalization, respectively. We showed that the punching method yielded comparable results to the conventional grinding method for two rice cultivars grown under various levels of P supply. Conclusions Compared with existing techniques, this protocol is more suited to an initial screening, enabling one researcher to determine the Pi contents of thousands of rice leaf samples within a few hours. inorganic phosphate microplate molybdenum blue assay rice Biology (General) Botany Supachitra Chadchawan verfasserin aut Juthamas Chaiwanon verfasserin aut In Applications in Plant Sciences Wiley, 2013 8(2020), 10, Seite n/a-n/a (DE-627)735133506 (DE-600)2699923-7 21680450 nnns volume:8 year:2020 number:10 pages:n/a-n/a https://doi.org/10.1002/aps3.11395 kostenfrei https://doaj.org/article/3f5f81ce46c849aeadf417e31f4b8d5a kostenfrei https://doi.org/10.1002/aps3.11395 kostenfrei https://doaj.org/toc/2168-0450 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2020 10 n/a-n/a
language	English
source	In Applications in Plant Sciences 8(2020), 10, Seite n/a-n/a volume:8 year:2020 number:10 pages:n/a-n/a
sourceStr	In Applications in Plant Sciences 8(2020), 10, Seite n/a-n/a volume:8 year:2020 number:10 pages:n/a-n/a
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	inorganic phosphate microplate molybdenum blue assay rice Biology (General) Botany
isfreeaccess_bool	true
container_title	Applications in Plant Sciences
authorswithroles_txt_mv	Sompop Pinit @@aut@@ Supachitra Chadchawan @@aut@@ Juthamas Chaiwanon @@aut@@
publishDateDaySort_date	2020-01-01T00:00:00Z
hierarchy_top_id	735133506
id	DOAJ044710305
language_de	englisch
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Determining inorganic phosphate (Pi) contents of plant tissues is crucial for evaluating plant P status. Here, we present a simple, high‐throughput colorimetric microplate technique to measure Pi contents in rice (Oryza sativa) leaf tissues, based on the molybdenum blue reaction. Methods and Results We used a hole puncher to sample small equal areas of leaf tissue for Pi extraction. We removed the leaf grinding and weighing steps, which are time‐consuming and normally required to release Pi from the tissues and to measure the biomass for data normalization, respectively. We showed that the punching method yielded comparable results to the conventional grinding method for two rice cultivars grown under various levels of P supply. 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callnumber-first	Q - Science
author	Sompop Pinit
spellingShingle	Sompop Pinit misc QH301-705.5 misc QK1-989 misc inorganic phosphate misc microplate misc molybdenum blue assay misc rice misc Biology (General) misc Botany A simple high‐throughput protocol for the extraction and quantification of inorganic phosphate in rice leaves
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topic_title	QH301-705.5 QK1-989 A simple high‐throughput protocol for the extraction and quantification of inorganic phosphate in rice leaves inorganic phosphate microplate molybdenum blue assay rice
topic	misc QH301-705.5 misc QK1-989 misc inorganic phosphate misc microplate misc molybdenum blue assay misc rice misc Biology (General) misc Botany
topic_unstemmed	misc QH301-705.5 misc QK1-989 misc inorganic phosphate misc microplate misc molybdenum blue assay misc rice misc Biology (General) misc Botany
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title	A simple high‐throughput protocol for the extraction and quantification of inorganic phosphate in rice leaves
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title_full	A simple high‐throughput protocol for the extraction and quantification of inorganic phosphate in rice leaves
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author_browse	Sompop Pinit Supachitra Chadchawan Juthamas Chaiwanon
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title_sort	simple high‐throughput protocol for the extraction and quantification of inorganic phosphate in rice leaves
callnumber	QH301-705.5
title_auth	A simple high‐throughput protocol for the extraction and quantification of inorganic phosphate in rice leaves
abstract	Premise Phosphorus (P) is an essential macronutrient that is often limited in agricultural systems. Determining inorganic phosphate (Pi) contents of plant tissues is crucial for evaluating plant P status. Here, we present a simple, high‐throughput colorimetric microplate technique to measure Pi contents in rice (Oryza sativa) leaf tissues, based on the molybdenum blue reaction. Methods and Results We used a hole puncher to sample small equal areas of leaf tissue for Pi extraction. We removed the leaf grinding and weighing steps, which are time‐consuming and normally required to release Pi from the tissues and to measure the biomass for data normalization, respectively. We showed that the punching method yielded comparable results to the conventional grinding method for two rice cultivars grown under various levels of P supply. Conclusions Compared with existing techniques, this protocol is more suited to an initial screening, enabling one researcher to determine the Pi contents of thousands of rice leaf samples within a few hours.
abstractGer	Premise Phosphorus (P) is an essential macronutrient that is often limited in agricultural systems. Determining inorganic phosphate (Pi) contents of plant tissues is crucial for evaluating plant P status. Here, we present a simple, high‐throughput colorimetric microplate technique to measure Pi contents in rice (Oryza sativa) leaf tissues, based on the molybdenum blue reaction. Methods and Results We used a hole puncher to sample small equal areas of leaf tissue for Pi extraction. We removed the leaf grinding and weighing steps, which are time‐consuming and normally required to release Pi from the tissues and to measure the biomass for data normalization, respectively. We showed that the punching method yielded comparable results to the conventional grinding method for two rice cultivars grown under various levels of P supply. Conclusions Compared with existing techniques, this protocol is more suited to an initial screening, enabling one researcher to determine the Pi contents of thousands of rice leaf samples within a few hours.
abstract_unstemmed	Premise Phosphorus (P) is an essential macronutrient that is often limited in agricultural systems. Determining inorganic phosphate (Pi) contents of plant tissues is crucial for evaluating plant P status. Here, we present a simple, high‐throughput colorimetric microplate technique to measure Pi contents in rice (Oryza sativa) leaf tissues, based on the molybdenum blue reaction. Methods and Results We used a hole puncher to sample small equal areas of leaf tissue for Pi extraction. We removed the leaf grinding and weighing steps, which are time‐consuming and normally required to release Pi from the tissues and to measure the biomass for data normalization, respectively. We showed that the punching method yielded comparable results to the conventional grinding method for two rice cultivars grown under various levels of P supply. Conclusions Compared with existing techniques, this protocol is more suited to an initial screening, enabling one researcher to determine the Pi contents of thousands of rice leaf samples within a few hours.
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title_short	A simple high‐throughput protocol for the extraction and quantification of inorganic phosphate in rice leaves
url	https://doi.org/10.1002/aps3.11395 https://doaj.org/article/3f5f81ce46c849aeadf417e31f4b8d5a https://doaj.org/toc/2168-0450
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A simple high‐throughput protocol for the extraction and quantification of inorganic phosphate in rice leaves

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