Downregulation of Immunosuppressive Molecules, PD-1 and PD-L1 but not PD-L2, in the Patients with Multiple Sclerosis

Programmed cell death-1 (PD-1) and its ligands, PD-L1 and PD-L2, have been regarded as important immune system regulatory molecules. The aberrant expression of the molecules has been related to several autoimmune disorders. This study is aimed to assess the mRNA expression level of PD-1, PD-L1, and...
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Autor*in:	Mohammad Reza Javan [verfasserIn] Saeed Aslani [verfasserIn] Mohammad Reza Zamani [verfasserIn] Javad Rostamnejad [verfasserIn] Milad Asadi [verfasserIn] Mahdi Farhoodi [verfasserIn] Mohammad Hossein Nicknam [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	Gene expression Multiple sclerosis Programmed cell death 1 receptor Programmed cell death 1 ligand 1 protein Programmed cell death 1 ligand 2 protein

Übergeordnetes Werk:	In: Iranian Journal of Allergy, Asthma and Immunology - Tehran University of Medical Sciences, 2020, 15(2016), 4
Übergeordnetes Werk:	volume:15 ; year:2016 ; number:4

Links:	Link aufrufen Link aufrufen Journal toc Journal toc

Katalog-ID:	DOAJ044886683

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520			\|a Programmed cell death-1 (PD-1) and its ligands, PD-L1 and PD-L2, have been regarded as important immune system regulatory molecules. The aberrant expression of the molecules has been related to several autoimmune disorders. This study is aimed to assess the mRNA expression level of PD-1, PD-L1, and PD-L2 molecules in the peripheral blood mononuclear mells (PBMCs) from multiple sclerosis (MS) patients. PBMCs were isolated from the whole blood of 50 MS and 50 healthy individuals. Total RNA content of the leukocytes was extracted. Then, cDNA was synthesized from the extracted RNA. Afterwards, quantitative analysis of PD-1, PD-L1 and PD-L2 was carried out through Real Time PCR using the TaqMan gene expression assays. Relative expression of PD-1 and PD-L1 in PBMCs from MS patients was significantly lower compared with the healthy control group (p=0.003 and 0.012, respectively). However, no significant difference was observed in the expression level of PD-L2 between patients and healthy individuals. Relative expression of PD-1 correlated with expanded disability status scale score (EDSS) of the patients (r=-0.763, p=0.008). Downregulation of the immunosuppressive molecules, PD-1 and PD-L1, may imply that over-activation of immune cells in multiple sclerosis occurs through signaling dysfunction of these molecules and PD-L2 plays no important role in this context.
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allfields	(DE-627)DOAJ044886683 (DE-599)DOAJc89a7914cdbf4c31a0a73795c5b532f5 DE-627 ger DE-627 rakwb eng Mohammad Reza Javan verfasserin aut Downregulation of Immunosuppressive Molecules, PD-1 and PD-L1 but not PD-L2, in the Patients with Multiple Sclerosis 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Programmed cell death-1 (PD-1) and its ligands, PD-L1 and PD-L2, have been regarded as important immune system regulatory molecules. The aberrant expression of the molecules has been related to several autoimmune disorders. This study is aimed to assess the mRNA expression level of PD-1, PD-L1, and PD-L2 molecules in the peripheral blood mononuclear mells (PBMCs) from multiple sclerosis (MS) patients. PBMCs were isolated from the whole blood of 50 MS and 50 healthy individuals. Total RNA content of the leukocytes was extracted. Then, cDNA was synthesized from the extracted RNA. Afterwards, quantitative analysis of PD-1, PD-L1 and PD-L2 was carried out through Real Time PCR using the TaqMan gene expression assays. Relative expression of PD-1 and PD-L1 in PBMCs from MS patients was significantly lower compared with the healthy control group (p=0.003 and 0.012, respectively). However, no significant difference was observed in the expression level of PD-L2 between patients and healthy individuals. Relative expression of PD-1 correlated with expanded disability status scale score (EDSS) of the patients (r=-0.763, p=0.008). Downregulation of the immunosuppressive molecules, PD-1 and PD-L1, may imply that over-activation of immune cells in multiple sclerosis occurs through signaling dysfunction of these molecules and PD-L2 plays no important role in this context. Gene expression Multiple sclerosis Programmed cell death 1 receptor Programmed cell death 1 ligand 1 protein Programmed cell death 1 ligand 2 protein Medicine R Saeed Aslani verfasserin aut Mohammad Reza Zamani verfasserin aut Javad Rostamnejad verfasserin aut Milad Asadi verfasserin aut Mahdi Farhoodi verfasserin aut Mohammad Hossein Nicknam verfasserin aut In Iranian Journal of Allergy, Asthma and Immunology Tehran University of Medical Sciences, 2020 15(2016), 4 (DE-627)545784166 (DE-600)2388260-8 17355249 nnns volume:15 year:2016 number:4 https://doaj.org/article/c89a7914cdbf4c31a0a73795c5b532f5 kostenfrei https://ijaai.tums.ac.ir/index.php/ijaai/article/view/632 kostenfrei https://doaj.org/toc/1735-1502 Journal toc kostenfrei https://doaj.org/toc/1735-5249 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2016 4
spelling	(DE-627)DOAJ044886683 (DE-599)DOAJc89a7914cdbf4c31a0a73795c5b532f5 DE-627 ger DE-627 rakwb eng Mohammad Reza Javan verfasserin aut Downregulation of Immunosuppressive Molecules, PD-1 and PD-L1 but not PD-L2, in the Patients with Multiple Sclerosis 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Programmed cell death-1 (PD-1) and its ligands, PD-L1 and PD-L2, have been regarded as important immune system regulatory molecules. The aberrant expression of the molecules has been related to several autoimmune disorders. This study is aimed to assess the mRNA expression level of PD-1, PD-L1, and PD-L2 molecules in the peripheral blood mononuclear mells (PBMCs) from multiple sclerosis (MS) patients. PBMCs were isolated from the whole blood of 50 MS and 50 healthy individuals. Total RNA content of the leukocytes was extracted. Then, cDNA was synthesized from the extracted RNA. Afterwards, quantitative analysis of PD-1, PD-L1 and PD-L2 was carried out through Real Time PCR using the TaqMan gene expression assays. Relative expression of PD-1 and PD-L1 in PBMCs from MS patients was significantly lower compared with the healthy control group (p=0.003 and 0.012, respectively). However, no significant difference was observed in the expression level of PD-L2 between patients and healthy individuals. Relative expression of PD-1 correlated with expanded disability status scale score (EDSS) of the patients (r=-0.763, p=0.008). Downregulation of the immunosuppressive molecules, PD-1 and PD-L1, may imply that over-activation of immune cells in multiple sclerosis occurs through signaling dysfunction of these molecules and PD-L2 plays no important role in this context. Gene expression Multiple sclerosis Programmed cell death 1 receptor Programmed cell death 1 ligand 1 protein Programmed cell death 1 ligand 2 protein Medicine R Saeed Aslani verfasserin aut Mohammad Reza Zamani verfasserin aut Javad Rostamnejad verfasserin aut Milad Asadi verfasserin aut Mahdi Farhoodi verfasserin aut Mohammad Hossein Nicknam verfasserin aut In Iranian Journal of Allergy, Asthma and Immunology Tehran University of Medical Sciences, 2020 15(2016), 4 (DE-627)545784166 (DE-600)2388260-8 17355249 nnns volume:15 year:2016 number:4 https://doaj.org/article/c89a7914cdbf4c31a0a73795c5b532f5 kostenfrei https://ijaai.tums.ac.ir/index.php/ijaai/article/view/632 kostenfrei https://doaj.org/toc/1735-1502 Journal toc kostenfrei https://doaj.org/toc/1735-5249 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2016 4
allfields_unstemmed	(DE-627)DOAJ044886683 (DE-599)DOAJc89a7914cdbf4c31a0a73795c5b532f5 DE-627 ger DE-627 rakwb eng Mohammad Reza Javan verfasserin aut Downregulation of Immunosuppressive Molecules, PD-1 and PD-L1 but not PD-L2, in the Patients with Multiple Sclerosis 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Programmed cell death-1 (PD-1) and its ligands, PD-L1 and PD-L2, have been regarded as important immune system regulatory molecules. The aberrant expression of the molecules has been related to several autoimmune disorders. This study is aimed to assess the mRNA expression level of PD-1, PD-L1, and PD-L2 molecules in the peripheral blood mononuclear mells (PBMCs) from multiple sclerosis (MS) patients. PBMCs were isolated from the whole blood of 50 MS and 50 healthy individuals. Total RNA content of the leukocytes was extracted. Then, cDNA was synthesized from the extracted RNA. Afterwards, quantitative analysis of PD-1, PD-L1 and PD-L2 was carried out through Real Time PCR using the TaqMan gene expression assays. Relative expression of PD-1 and PD-L1 in PBMCs from MS patients was significantly lower compared with the healthy control group (p=0.003 and 0.012, respectively). However, no significant difference was observed in the expression level of PD-L2 between patients and healthy individuals. Relative expression of PD-1 correlated with expanded disability status scale score (EDSS) of the patients (r=-0.763, p=0.008). Downregulation of the immunosuppressive molecules, PD-1 and PD-L1, may imply that over-activation of immune cells in multiple sclerosis occurs through signaling dysfunction of these molecules and PD-L2 plays no important role in this context. Gene expression Multiple sclerosis Programmed cell death 1 receptor Programmed cell death 1 ligand 1 protein Programmed cell death 1 ligand 2 protein Medicine R Saeed Aslani verfasserin aut Mohammad Reza Zamani verfasserin aut Javad Rostamnejad verfasserin aut Milad Asadi verfasserin aut Mahdi Farhoodi verfasserin aut Mohammad Hossein Nicknam verfasserin aut In Iranian Journal of Allergy, Asthma and Immunology Tehran University of Medical Sciences, 2020 15(2016), 4 (DE-627)545784166 (DE-600)2388260-8 17355249 nnns volume:15 year:2016 number:4 https://doaj.org/article/c89a7914cdbf4c31a0a73795c5b532f5 kostenfrei https://ijaai.tums.ac.ir/index.php/ijaai/article/view/632 kostenfrei https://doaj.org/toc/1735-1502 Journal toc kostenfrei https://doaj.org/toc/1735-5249 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2016 4
allfieldsGer	(DE-627)DOAJ044886683 (DE-599)DOAJc89a7914cdbf4c31a0a73795c5b532f5 DE-627 ger DE-627 rakwb eng Mohammad Reza Javan verfasserin aut Downregulation of Immunosuppressive Molecules, PD-1 and PD-L1 but not PD-L2, in the Patients with Multiple Sclerosis 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Programmed cell death-1 (PD-1) and its ligands, PD-L1 and PD-L2, have been regarded as important immune system regulatory molecules. The aberrant expression of the molecules has been related to several autoimmune disorders. This study is aimed to assess the mRNA expression level of PD-1, PD-L1, and PD-L2 molecules in the peripheral blood mononuclear mells (PBMCs) from multiple sclerosis (MS) patients. PBMCs were isolated from the whole blood of 50 MS and 50 healthy individuals. Total RNA content of the leukocytes was extracted. Then, cDNA was synthesized from the extracted RNA. Afterwards, quantitative analysis of PD-1, PD-L1 and PD-L2 was carried out through Real Time PCR using the TaqMan gene expression assays. Relative expression of PD-1 and PD-L1 in PBMCs from MS patients was significantly lower compared with the healthy control group (p=0.003 and 0.012, respectively). However, no significant difference was observed in the expression level of PD-L2 between patients and healthy individuals. Relative expression of PD-1 correlated with expanded disability status scale score (EDSS) of the patients (r=-0.763, p=0.008). Downregulation of the immunosuppressive molecules, PD-1 and PD-L1, may imply that over-activation of immune cells in multiple sclerosis occurs through signaling dysfunction of these molecules and PD-L2 plays no important role in this context. Gene expression Multiple sclerosis Programmed cell death 1 receptor Programmed cell death 1 ligand 1 protein Programmed cell death 1 ligand 2 protein Medicine R Saeed Aslani verfasserin aut Mohammad Reza Zamani verfasserin aut Javad Rostamnejad verfasserin aut Milad Asadi verfasserin aut Mahdi Farhoodi verfasserin aut Mohammad Hossein Nicknam verfasserin aut In Iranian Journal of Allergy, Asthma and Immunology Tehran University of Medical Sciences, 2020 15(2016), 4 (DE-627)545784166 (DE-600)2388260-8 17355249 nnns volume:15 year:2016 number:4 https://doaj.org/article/c89a7914cdbf4c31a0a73795c5b532f5 kostenfrei https://ijaai.tums.ac.ir/index.php/ijaai/article/view/632 kostenfrei https://doaj.org/toc/1735-1502 Journal toc kostenfrei https://doaj.org/toc/1735-5249 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2016 4
allfieldsSound	(DE-627)DOAJ044886683 (DE-599)DOAJc89a7914cdbf4c31a0a73795c5b532f5 DE-627 ger DE-627 rakwb eng Mohammad Reza Javan verfasserin aut Downregulation of Immunosuppressive Molecules, PD-1 and PD-L1 but not PD-L2, in the Patients with Multiple Sclerosis 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Programmed cell death-1 (PD-1) and its ligands, PD-L1 and PD-L2, have been regarded as important immune system regulatory molecules. The aberrant expression of the molecules has been related to several autoimmune disorders. This study is aimed to assess the mRNA expression level of PD-1, PD-L1, and PD-L2 molecules in the peripheral blood mononuclear mells (PBMCs) from multiple sclerosis (MS) patients. PBMCs were isolated from the whole blood of 50 MS and 50 healthy individuals. Total RNA content of the leukocytes was extracted. Then, cDNA was synthesized from the extracted RNA. Afterwards, quantitative analysis of PD-1, PD-L1 and PD-L2 was carried out through Real Time PCR using the TaqMan gene expression assays. Relative expression of PD-1 and PD-L1 in PBMCs from MS patients was significantly lower compared with the healthy control group (p=0.003 and 0.012, respectively). However, no significant difference was observed in the expression level of PD-L2 between patients and healthy individuals. Relative expression of PD-1 correlated with expanded disability status scale score (EDSS) of the patients (r=-0.763, p=0.008). Downregulation of the immunosuppressive molecules, PD-1 and PD-L1, may imply that over-activation of immune cells in multiple sclerosis occurs through signaling dysfunction of these molecules and PD-L2 plays no important role in this context. Gene expression Multiple sclerosis Programmed cell death 1 receptor Programmed cell death 1 ligand 1 protein Programmed cell death 1 ligand 2 protein Medicine R Saeed Aslani verfasserin aut Mohammad Reza Zamani verfasserin aut Javad Rostamnejad verfasserin aut Milad Asadi verfasserin aut Mahdi Farhoodi verfasserin aut Mohammad Hossein Nicknam verfasserin aut In Iranian Journal of Allergy, Asthma and Immunology Tehran University of Medical Sciences, 2020 15(2016), 4 (DE-627)545784166 (DE-600)2388260-8 17355249 nnns volume:15 year:2016 number:4 https://doaj.org/article/c89a7914cdbf4c31a0a73795c5b532f5 kostenfrei https://ijaai.tums.ac.ir/index.php/ijaai/article/view/632 kostenfrei https://doaj.org/toc/1735-1502 Journal toc kostenfrei https://doaj.org/toc/1735-5249 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2016 4
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author	Mohammad Reza Javan
spellingShingle	Mohammad Reza Javan misc Gene expression misc Multiple sclerosis misc Programmed cell death 1 receptor misc Programmed cell death 1 ligand 1 protein misc Programmed cell death 1 ligand 2 protein misc Medicine misc R Downregulation of Immunosuppressive Molecules, PD-1 and PD-L1 but not PD-L2, in the Patients with Multiple Sclerosis
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topic_title	Downregulation of Immunosuppressive Molecules, PD-1 and PD-L1 but not PD-L2, in the Patients with Multiple Sclerosis Gene expression Multiple sclerosis Programmed cell death 1 receptor Programmed cell death 1 ligand 1 protein Programmed cell death 1 ligand 2 protein
topic	misc Gene expression misc Multiple sclerosis misc Programmed cell death 1 receptor misc Programmed cell death 1 ligand 1 protein misc Programmed cell death 1 ligand 2 protein misc Medicine misc R
topic_unstemmed	misc Gene expression misc Multiple sclerosis misc Programmed cell death 1 receptor misc Programmed cell death 1 ligand 1 protein misc Programmed cell death 1 ligand 2 protein misc Medicine misc R
topic_browse	misc Gene expression misc Multiple sclerosis misc Programmed cell death 1 receptor misc Programmed cell death 1 ligand 1 protein misc Programmed cell death 1 ligand 2 protein misc Medicine misc R
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title	Downregulation of Immunosuppressive Molecules, PD-1 and PD-L1 but not PD-L2, in the Patients with Multiple Sclerosis
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title_full	Downregulation of Immunosuppressive Molecules, PD-1 and PD-L1 but not PD-L2, in the Patients with Multiple Sclerosis
author_sort	Mohammad Reza Javan
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author_browse	Mohammad Reza Javan Saeed Aslani Mohammad Reza Zamani Javad Rostamnejad Milad Asadi Mahdi Farhoodi Mohammad Hossein Nicknam
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title_sort	downregulation of immunosuppressive molecules, pd-1 and pd-l1 but not pd-l2, in the patients with multiple sclerosis
title_auth	Downregulation of Immunosuppressive Molecules, PD-1 and PD-L1 but not PD-L2, in the Patients with Multiple Sclerosis
abstract	Programmed cell death-1 (PD-1) and its ligands, PD-L1 and PD-L2, have been regarded as important immune system regulatory molecules. The aberrant expression of the molecules has been related to several autoimmune disorders. This study is aimed to assess the mRNA expression level of PD-1, PD-L1, and PD-L2 molecules in the peripheral blood mononuclear mells (PBMCs) from multiple sclerosis (MS) patients. PBMCs were isolated from the whole blood of 50 MS and 50 healthy individuals. Total RNA content of the leukocytes was extracted. Then, cDNA was synthesized from the extracted RNA. Afterwards, quantitative analysis of PD-1, PD-L1 and PD-L2 was carried out through Real Time PCR using the TaqMan gene expression assays. Relative expression of PD-1 and PD-L1 in PBMCs from MS patients was significantly lower compared with the healthy control group (p=0.003 and 0.012, respectively). However, no significant difference was observed in the expression level of PD-L2 between patients and healthy individuals. Relative expression of PD-1 correlated with expanded disability status scale score (EDSS) of the patients (r=-0.763, p=0.008). Downregulation of the immunosuppressive molecules, PD-1 and PD-L1, may imply that over-activation of immune cells in multiple sclerosis occurs through signaling dysfunction of these molecules and PD-L2 plays no important role in this context.
abstractGer	Programmed cell death-1 (PD-1) and its ligands, PD-L1 and PD-L2, have been regarded as important immune system regulatory molecules. The aberrant expression of the molecules has been related to several autoimmune disorders. This study is aimed to assess the mRNA expression level of PD-1, PD-L1, and PD-L2 molecules in the peripheral blood mononuclear mells (PBMCs) from multiple sclerosis (MS) patients. PBMCs were isolated from the whole blood of 50 MS and 50 healthy individuals. Total RNA content of the leukocytes was extracted. Then, cDNA was synthesized from the extracted RNA. Afterwards, quantitative analysis of PD-1, PD-L1 and PD-L2 was carried out through Real Time PCR using the TaqMan gene expression assays. Relative expression of PD-1 and PD-L1 in PBMCs from MS patients was significantly lower compared with the healthy control group (p=0.003 and 0.012, respectively). However, no significant difference was observed in the expression level of PD-L2 between patients and healthy individuals. Relative expression of PD-1 correlated with expanded disability status scale score (EDSS) of the patients (r=-0.763, p=0.008). Downregulation of the immunosuppressive molecules, PD-1 and PD-L1, may imply that over-activation of immune cells in multiple sclerosis occurs through signaling dysfunction of these molecules and PD-L2 plays no important role in this context.
abstract_unstemmed	Programmed cell death-1 (PD-1) and its ligands, PD-L1 and PD-L2, have been regarded as important immune system regulatory molecules. The aberrant expression of the molecules has been related to several autoimmune disorders. This study is aimed to assess the mRNA expression level of PD-1, PD-L1, and PD-L2 molecules in the peripheral blood mononuclear mells (PBMCs) from multiple sclerosis (MS) patients. PBMCs were isolated from the whole blood of 50 MS and 50 healthy individuals. Total RNA content of the leukocytes was extracted. Then, cDNA was synthesized from the extracted RNA. Afterwards, quantitative analysis of PD-1, PD-L1 and PD-L2 was carried out through Real Time PCR using the TaqMan gene expression assays. Relative expression of PD-1 and PD-L1 in PBMCs from MS patients was significantly lower compared with the healthy control group (p=0.003 and 0.012, respectively). However, no significant difference was observed in the expression level of PD-L2 between patients and healthy individuals. Relative expression of PD-1 correlated with expanded disability status scale score (EDSS) of the patients (r=-0.763, p=0.008). Downregulation of the immunosuppressive molecules, PD-1 and PD-L1, may imply that over-activation of immune cells in multiple sclerosis occurs through signaling dysfunction of these molecules and PD-L2 plays no important role in this context.
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title_short	Downregulation of Immunosuppressive Molecules, PD-1 and PD-L1 but not PD-L2, in the Patients with Multiple Sclerosis
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up_date	2024-07-04T00:51:39.256Z
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Downregulation of Immunosuppressive Molecules, PD-1 and PD-L1 but not PD-L2, in the Patients with Multiple Sclerosis

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