Antioxidant, Antiproliferative and Apoptosis-Inducing Efficacy of Fractions from <i<Cassia fistula</i< L. Leaves

Cassia fistula L. is a highly admirable traditional medicinal plant used for the treatment of various diseases and disorders. The present study was performed to divulge the antioxidant, antiproliferative, and apoptosis-inducing efficacy of fractions from <i<C. fistula</i< leaves. The hexane (CaLH fraction), chloroform (CaLC fraction), ethyl acetate (CaLE fraction), <i<n</i<-butanol (CaLB fraction), and aqueous (CaLA fraction) were sequentially fractionated from 80% methanolic (CaLM extract) of <i<C. fistula</i< leaves. The CaLE fraction was fractionated using column chromatography to yield a pure compound, which was characterized as Epiafzelechin (CFL1) based on <sup<1</sup<H, <sup<13</sup<C, and DEPT135 NMR. Among these fractions, CaLE and isolated CFL1 fractions exhibited an effective antioxidant potential in Ferric ion reducing power, (2,2’-azino-bis (3-ethylbenzothiazoline -6-sulfonic acid)) cation radical scavenging, and nitric oxide radical scavenging assays. Epiafzelechin was investigated for its antiproliferative effects against MG-63 (osteosarcoma), IMR-32 (neuroblastoma), and PC-3 (prostate adenocarcinoma), and was found to inhibit cell proliferation with a GI<sub<50</sub< value of 8.73, 9.15, and 11.8 μM respectively. MG-63 cells underwent apoptotic cell death on treatment with Epiafzelechin as the cells showed the formation of apoptotic bodies, enhanced reactive oxygen species (ROS) generation, mitochondrial membrane depolarization along with an increase in early apoptotic cell population analyzed using Annexin V-FITC/PI double staining assay. Cells showed cell cycle arrest at the G<sub<0</sub</G<sub<1</sub< phase accompanied by a downregulation in the expression levels of p-Akt (Protein kinase B), p-GSK-3β (Glycogen synthase kinase-3 beta), and Bcl-xl (B-cell lymphoma-extra large) proteins. RT-PCR (Real time-polymerase chain reaction) analysis revealed downregulation in the gene expression level of β-catenin and CDK2 (cyclin-dependent kinases-2) while it upregulated the expression level of caspase-8 and p53 genes in MG-63 cells. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Sandeep Kaur [verfasserIn] Ajay Kumar [verfasserIn] Sharad Thakur [verfasserIn] Kapil Kumar [verfasserIn] Ritika Sharma [verfasserIn] Anket Sharma [verfasserIn] Prabhpreet Singh [verfasserIn] Upendra Sharma [verfasserIn] Subodh Kumar [verfasserIn] Marco Landi [verfasserIn] Marián Brestič [verfasserIn] Satwinderjeet Kaur [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	antioxidant antiproliferative apoptosis osteosarcoma

Übergeordnetes Werk:	In: Antioxidants - MDPI AG, 2013, 9(2020), 2, p 173
Übergeordnetes Werk:	volume:9 ; year:2020 ; number:2, p 173

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/antiox9020173

Katalog-ID:	DOAJ045369879

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allfields	10.3390/antiox9020173 doi (DE-627)DOAJ045369879 (DE-599)DOAJde74e498cf484b28a636fdcab59f46e2 DE-627 ger DE-627 rakwb eng RM1-950 Sandeep Kaur verfasserin aut Antioxidant, Antiproliferative and Apoptosis-Inducing Efficacy of Fractions from <i<Cassia fistula</i< L. Leaves 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cassia fistula L. is a highly admirable traditional medicinal plant used for the treatment of various diseases and disorders. The present study was performed to divulge the antioxidant, antiproliferative, and apoptosis-inducing efficacy of fractions from <i<C. fistula</i< leaves. The hexane (CaLH fraction), chloroform (CaLC fraction), ethyl acetate (CaLE fraction), <i<n</i<-butanol (CaLB fraction), and aqueous (CaLA fraction) were sequentially fractionated from 80% methanolic (CaLM extract) of <i<C. fistula</i< leaves. The CaLE fraction was fractionated using column chromatography to yield a pure compound, which was characterized as Epiafzelechin (CFL1) based on <sup<1</sup<H, <sup<13</sup<C, and DEPT135 NMR. Among these fractions, CaLE and isolated CFL1 fractions exhibited an effective antioxidant potential in Ferric ion reducing power, (2,2’-azino-bis (3-ethylbenzothiazoline -6-sulfonic acid)) cation radical scavenging, and nitric oxide radical scavenging assays. Epiafzelechin was investigated for its antiproliferative effects against MG-63 (osteosarcoma), IMR-32 (neuroblastoma), and PC-3 (prostate adenocarcinoma), and was found to inhibit cell proliferation with a GI<sub<50</sub< value of 8.73, 9.15, and 11.8 μM respectively. MG-63 cells underwent apoptotic cell death on treatment with Epiafzelechin as the cells showed the formation of apoptotic bodies, enhanced reactive oxygen species (ROS) generation, mitochondrial membrane depolarization along with an increase in early apoptotic cell population analyzed using Annexin V-FITC/PI double staining assay. Cells showed cell cycle arrest at the G<sub<0</sub</G<sub<1</sub< phase accompanied by a downregulation in the expression levels of p-Akt (Protein kinase B), p-GSK-3β (Glycogen synthase kinase-3 beta), and Bcl-xl (B-cell lymphoma-extra large) proteins. RT-PCR (Real time-polymerase chain reaction) analysis revealed downregulation in the gene expression level of β-catenin and CDK2 (cyclin-dependent kinases-2) while it upregulated the expression level of caspase-8 and p53 genes in MG-63 cells. <i<cassia fistula</i< antioxidant antiproliferative apoptosis osteosarcoma Therapeutics. Pharmacology Ajay Kumar verfasserin aut Sharad Thakur verfasserin aut Kapil Kumar verfasserin aut Ritika Sharma verfasserin aut Anket Sharma verfasserin aut Prabhpreet Singh verfasserin aut Upendra Sharma verfasserin aut Subodh Kumar verfasserin aut Marco Landi verfasserin aut Marián Brestič verfasserin aut Satwinderjeet Kaur verfasserin aut In Antioxidants MDPI AG, 2013 9(2020), 2, p 173 (DE-627)737287578 (DE-600)2704216-9 20763921 nnns volume:9 year:2020 number:2, p 173 https://doi.org/10.3390/antiox9020173 kostenfrei https://doaj.org/article/de74e498cf484b28a636fdcab59f46e2 kostenfrei https://www.mdpi.com/2076-3921/9/2/173 kostenfrei https://doaj.org/toc/2076-3921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 2, p 173
spelling	10.3390/antiox9020173 doi (DE-627)DOAJ045369879 (DE-599)DOAJde74e498cf484b28a636fdcab59f46e2 DE-627 ger DE-627 rakwb eng RM1-950 Sandeep Kaur verfasserin aut Antioxidant, Antiproliferative and Apoptosis-Inducing Efficacy of Fractions from <i<Cassia fistula</i< L. Leaves 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cassia fistula L. is a highly admirable traditional medicinal plant used for the treatment of various diseases and disorders. The present study was performed to divulge the antioxidant, antiproliferative, and apoptosis-inducing efficacy of fractions from <i<C. fistula</i< leaves. The hexane (CaLH fraction), chloroform (CaLC fraction), ethyl acetate (CaLE fraction), <i<n</i<-butanol (CaLB fraction), and aqueous (CaLA fraction) were sequentially fractionated from 80% methanolic (CaLM extract) of <i<C. fistula</i< leaves. The CaLE fraction was fractionated using column chromatography to yield a pure compound, which was characterized as Epiafzelechin (CFL1) based on <sup<1</sup<H, <sup<13</sup<C, and DEPT135 NMR. Among these fractions, CaLE and isolated CFL1 fractions exhibited an effective antioxidant potential in Ferric ion reducing power, (2,2’-azino-bis (3-ethylbenzothiazoline -6-sulfonic acid)) cation radical scavenging, and nitric oxide radical scavenging assays. Epiafzelechin was investigated for its antiproliferative effects against MG-63 (osteosarcoma), IMR-32 (neuroblastoma), and PC-3 (prostate adenocarcinoma), and was found to inhibit cell proliferation with a GI<sub<50</sub< value of 8.73, 9.15, and 11.8 μM respectively. MG-63 cells underwent apoptotic cell death on treatment with Epiafzelechin as the cells showed the formation of apoptotic bodies, enhanced reactive oxygen species (ROS) generation, mitochondrial membrane depolarization along with an increase in early apoptotic cell population analyzed using Annexin V-FITC/PI double staining assay. Cells showed cell cycle arrest at the G<sub<0</sub</G<sub<1</sub< phase accompanied by a downregulation in the expression levels of p-Akt (Protein kinase B), p-GSK-3β (Glycogen synthase kinase-3 beta), and Bcl-xl (B-cell lymphoma-extra large) proteins. RT-PCR (Real time-polymerase chain reaction) analysis revealed downregulation in the gene expression level of β-catenin and CDK2 (cyclin-dependent kinases-2) while it upregulated the expression level of caspase-8 and p53 genes in MG-63 cells. <i<cassia fistula</i< antioxidant antiproliferative apoptosis osteosarcoma Therapeutics. Pharmacology Ajay Kumar verfasserin aut Sharad Thakur verfasserin aut Kapil Kumar verfasserin aut Ritika Sharma verfasserin aut Anket Sharma verfasserin aut Prabhpreet Singh verfasserin aut Upendra Sharma verfasserin aut Subodh Kumar verfasserin aut Marco Landi verfasserin aut Marián Brestič verfasserin aut Satwinderjeet Kaur verfasserin aut In Antioxidants MDPI AG, 2013 9(2020), 2, p 173 (DE-627)737287578 (DE-600)2704216-9 20763921 nnns volume:9 year:2020 number:2, p 173 https://doi.org/10.3390/antiox9020173 kostenfrei https://doaj.org/article/de74e498cf484b28a636fdcab59f46e2 kostenfrei https://www.mdpi.com/2076-3921/9/2/173 kostenfrei https://doaj.org/toc/2076-3921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 2, p 173
allfields_unstemmed	10.3390/antiox9020173 doi (DE-627)DOAJ045369879 (DE-599)DOAJde74e498cf484b28a636fdcab59f46e2 DE-627 ger DE-627 rakwb eng RM1-950 Sandeep Kaur verfasserin aut Antioxidant, Antiproliferative and Apoptosis-Inducing Efficacy of Fractions from <i<Cassia fistula</i< L. Leaves 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cassia fistula L. is a highly admirable traditional medicinal plant used for the treatment of various diseases and disorders. The present study was performed to divulge the antioxidant, antiproliferative, and apoptosis-inducing efficacy of fractions from <i<C. fistula</i< leaves. The hexane (CaLH fraction), chloroform (CaLC fraction), ethyl acetate (CaLE fraction), <i<n</i<-butanol (CaLB fraction), and aqueous (CaLA fraction) were sequentially fractionated from 80% methanolic (CaLM extract) of <i<C. fistula</i< leaves. The CaLE fraction was fractionated using column chromatography to yield a pure compound, which was characterized as Epiafzelechin (CFL1) based on <sup<1</sup<H, <sup<13</sup<C, and DEPT135 NMR. Among these fractions, CaLE and isolated CFL1 fractions exhibited an effective antioxidant potential in Ferric ion reducing power, (2,2’-azino-bis (3-ethylbenzothiazoline -6-sulfonic acid)) cation radical scavenging, and nitric oxide radical scavenging assays. Epiafzelechin was investigated for its antiproliferative effects against MG-63 (osteosarcoma), IMR-32 (neuroblastoma), and PC-3 (prostate adenocarcinoma), and was found to inhibit cell proliferation with a GI<sub<50</sub< value of 8.73, 9.15, and 11.8 μM respectively. MG-63 cells underwent apoptotic cell death on treatment with Epiafzelechin as the cells showed the formation of apoptotic bodies, enhanced reactive oxygen species (ROS) generation, mitochondrial membrane depolarization along with an increase in early apoptotic cell population analyzed using Annexin V-FITC/PI double staining assay. Cells showed cell cycle arrest at the G<sub<0</sub</G<sub<1</sub< phase accompanied by a downregulation in the expression levels of p-Akt (Protein kinase B), p-GSK-3β (Glycogen synthase kinase-3 beta), and Bcl-xl (B-cell lymphoma-extra large) proteins. RT-PCR (Real time-polymerase chain reaction) analysis revealed downregulation in the gene expression level of β-catenin and CDK2 (cyclin-dependent kinases-2) while it upregulated the expression level of caspase-8 and p53 genes in MG-63 cells. <i<cassia fistula</i< antioxidant antiproliferative apoptosis osteosarcoma Therapeutics. Pharmacology Ajay Kumar verfasserin aut Sharad Thakur verfasserin aut Kapil Kumar verfasserin aut Ritika Sharma verfasserin aut Anket Sharma verfasserin aut Prabhpreet Singh verfasserin aut Upendra Sharma verfasserin aut Subodh Kumar verfasserin aut Marco Landi verfasserin aut Marián Brestič verfasserin aut Satwinderjeet Kaur verfasserin aut In Antioxidants MDPI AG, 2013 9(2020), 2, p 173 (DE-627)737287578 (DE-600)2704216-9 20763921 nnns volume:9 year:2020 number:2, p 173 https://doi.org/10.3390/antiox9020173 kostenfrei https://doaj.org/article/de74e498cf484b28a636fdcab59f46e2 kostenfrei https://www.mdpi.com/2076-3921/9/2/173 kostenfrei https://doaj.org/toc/2076-3921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 2, p 173
allfieldsGer	10.3390/antiox9020173 doi (DE-627)DOAJ045369879 (DE-599)DOAJde74e498cf484b28a636fdcab59f46e2 DE-627 ger DE-627 rakwb eng RM1-950 Sandeep Kaur verfasserin aut Antioxidant, Antiproliferative and Apoptosis-Inducing Efficacy of Fractions from <i<Cassia fistula</i< L. Leaves 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cassia fistula L. is a highly admirable traditional medicinal plant used for the treatment of various diseases and disorders. The present study was performed to divulge the antioxidant, antiproliferative, and apoptosis-inducing efficacy of fractions from <i<C. fistula</i< leaves. The hexane (CaLH fraction), chloroform (CaLC fraction), ethyl acetate (CaLE fraction), <i<n</i<-butanol (CaLB fraction), and aqueous (CaLA fraction) were sequentially fractionated from 80% methanolic (CaLM extract) of <i<C. fistula</i< leaves. The CaLE fraction was fractionated using column chromatography to yield a pure compound, which was characterized as Epiafzelechin (CFL1) based on <sup<1</sup<H, <sup<13</sup<C, and DEPT135 NMR. Among these fractions, CaLE and isolated CFL1 fractions exhibited an effective antioxidant potential in Ferric ion reducing power, (2,2’-azino-bis (3-ethylbenzothiazoline -6-sulfonic acid)) cation radical scavenging, and nitric oxide radical scavenging assays. Epiafzelechin was investigated for its antiproliferative effects against MG-63 (osteosarcoma), IMR-32 (neuroblastoma), and PC-3 (prostate adenocarcinoma), and was found to inhibit cell proliferation with a GI<sub<50</sub< value of 8.73, 9.15, and 11.8 μM respectively. MG-63 cells underwent apoptotic cell death on treatment with Epiafzelechin as the cells showed the formation of apoptotic bodies, enhanced reactive oxygen species (ROS) generation, mitochondrial membrane depolarization along with an increase in early apoptotic cell population analyzed using Annexin V-FITC/PI double staining assay. Cells showed cell cycle arrest at the G<sub<0</sub</G<sub<1</sub< phase accompanied by a downregulation in the expression levels of p-Akt (Protein kinase B), p-GSK-3β (Glycogen synthase kinase-3 beta), and Bcl-xl (B-cell lymphoma-extra large) proteins. RT-PCR (Real time-polymerase chain reaction) analysis revealed downregulation in the gene expression level of β-catenin and CDK2 (cyclin-dependent kinases-2) while it upregulated the expression level of caspase-8 and p53 genes in MG-63 cells. <i<cassia fistula</i< antioxidant antiproliferative apoptosis osteosarcoma Therapeutics. Pharmacology Ajay Kumar verfasserin aut Sharad Thakur verfasserin aut Kapil Kumar verfasserin aut Ritika Sharma verfasserin aut Anket Sharma verfasserin aut Prabhpreet Singh verfasserin aut Upendra Sharma verfasserin aut Subodh Kumar verfasserin aut Marco Landi verfasserin aut Marián Brestič verfasserin aut Satwinderjeet Kaur verfasserin aut In Antioxidants MDPI AG, 2013 9(2020), 2, p 173 (DE-627)737287578 (DE-600)2704216-9 20763921 nnns volume:9 year:2020 number:2, p 173 https://doi.org/10.3390/antiox9020173 kostenfrei https://doaj.org/article/de74e498cf484b28a636fdcab59f46e2 kostenfrei https://www.mdpi.com/2076-3921/9/2/173 kostenfrei https://doaj.org/toc/2076-3921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 2, p 173
allfieldsSound	10.3390/antiox9020173 doi (DE-627)DOAJ045369879 (DE-599)DOAJde74e498cf484b28a636fdcab59f46e2 DE-627 ger DE-627 rakwb eng RM1-950 Sandeep Kaur verfasserin aut Antioxidant, Antiproliferative and Apoptosis-Inducing Efficacy of Fractions from <i<Cassia fistula</i< L. Leaves 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Cassia fistula L. is a highly admirable traditional medicinal plant used for the treatment of various diseases and disorders. The present study was performed to divulge the antioxidant, antiproliferative, and apoptosis-inducing efficacy of fractions from <i<C. fistula</i< leaves. The hexane (CaLH fraction), chloroform (CaLC fraction), ethyl acetate (CaLE fraction), <i<n</i<-butanol (CaLB fraction), and aqueous (CaLA fraction) were sequentially fractionated from 80% methanolic (CaLM extract) of <i<C. fistula</i< leaves. The CaLE fraction was fractionated using column chromatography to yield a pure compound, which was characterized as Epiafzelechin (CFL1) based on <sup<1</sup<H, <sup<13</sup<C, and DEPT135 NMR. Among these fractions, CaLE and isolated CFL1 fractions exhibited an effective antioxidant potential in Ferric ion reducing power, (2,2’-azino-bis (3-ethylbenzothiazoline -6-sulfonic acid)) cation radical scavenging, and nitric oxide radical scavenging assays. Epiafzelechin was investigated for its antiproliferative effects against MG-63 (osteosarcoma), IMR-32 (neuroblastoma), and PC-3 (prostate adenocarcinoma), and was found to inhibit cell proliferation with a GI<sub<50</sub< value of 8.73, 9.15, and 11.8 μM respectively. MG-63 cells underwent apoptotic cell death on treatment with Epiafzelechin as the cells showed the formation of apoptotic bodies, enhanced reactive oxygen species (ROS) generation, mitochondrial membrane depolarization along with an increase in early apoptotic cell population analyzed using Annexin V-FITC/PI double staining assay. Cells showed cell cycle arrest at the G<sub<0</sub</G<sub<1</sub< phase accompanied by a downregulation in the expression levels of p-Akt (Protein kinase B), p-GSK-3β (Glycogen synthase kinase-3 beta), and Bcl-xl (B-cell lymphoma-extra large) proteins. RT-PCR (Real time-polymerase chain reaction) analysis revealed downregulation in the gene expression level of β-catenin and CDK2 (cyclin-dependent kinases-2) while it upregulated the expression level of caspase-8 and p53 genes in MG-63 cells. <i<cassia fistula</i< antioxidant antiproliferative apoptosis osteosarcoma Therapeutics. Pharmacology Ajay Kumar verfasserin aut Sharad Thakur verfasserin aut Kapil Kumar verfasserin aut Ritika Sharma verfasserin aut Anket Sharma verfasserin aut Prabhpreet Singh verfasserin aut Upendra Sharma verfasserin aut Subodh Kumar verfasserin aut Marco Landi verfasserin aut Marián Brestič verfasserin aut Satwinderjeet Kaur verfasserin aut In Antioxidants MDPI AG, 2013 9(2020), 2, p 173 (DE-627)737287578 (DE-600)2704216-9 20763921 nnns volume:9 year:2020 number:2, p 173 https://doi.org/10.3390/antiox9020173 kostenfrei https://doaj.org/article/de74e498cf484b28a636fdcab59f46e2 kostenfrei https://www.mdpi.com/2076-3921/9/2/173 kostenfrei https://doaj.org/toc/2076-3921 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2020 2, p 173
language	English
source	In Antioxidants 9(2020), 2, p 173 volume:9 year:2020 number:2, p 173
sourceStr	In Antioxidants 9(2020), 2, p 173 volume:9 year:2020 number:2, p 173
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	<i<cassia fistula</i< antioxidant antiproliferative apoptosis osteosarcoma Therapeutics. Pharmacology
isfreeaccess_bool	true
container_title	Antioxidants
authorswithroles_txt_mv	Sandeep Kaur @@aut@@ Ajay Kumar @@aut@@ Sharad Thakur @@aut@@ Kapil Kumar @@aut@@ Ritika Sharma @@aut@@ Anket Sharma @@aut@@ Prabhpreet Singh @@aut@@ Upendra Sharma @@aut@@ Subodh Kumar @@aut@@ Marco Landi @@aut@@ Marián Brestič @@aut@@ Satwinderjeet Kaur @@aut@@
publishDateDaySort_date	2020-01-01T00:00:00Z
hierarchy_top_id	737287578
id	DOAJ045369879
language_de	englisch
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callnumber-first	R - Medicine
author	Sandeep Kaur
spellingShingle	Sandeep Kaur misc RM1-950 misc <i<cassia fistula</i< misc antioxidant misc antiproliferative misc apoptosis misc osteosarcoma misc Therapeutics. Pharmacology Antioxidant, Antiproliferative and Apoptosis-Inducing Efficacy of Fractions from <i<Cassia fistula</i< L. Leaves
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topic_title	RM1-950 Antioxidant, Antiproliferative and Apoptosis-Inducing Efficacy of Fractions from <i<Cassia fistula</i< L. Leaves <i<cassia fistula</i< antioxidant antiproliferative apoptosis osteosarcoma
topic	misc RM1-950 misc <i<cassia fistula</i< misc antioxidant misc antiproliferative misc apoptosis misc osteosarcoma misc Therapeutics. Pharmacology
topic_unstemmed	misc RM1-950 misc <i<cassia fistula</i< misc antioxidant misc antiproliferative misc apoptosis misc osteosarcoma misc Therapeutics. Pharmacology
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title	Antioxidant, Antiproliferative and Apoptosis-Inducing Efficacy of Fractions from <i<Cassia fistula</i< L. Leaves
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title_full	Antioxidant, Antiproliferative and Apoptosis-Inducing Efficacy of Fractions from <i<Cassia fistula</i< L. Leaves
author_sort	Sandeep Kaur
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author_browse	Sandeep Kaur Ajay Kumar Sharad Thakur Kapil Kumar Ritika Sharma Anket Sharma Prabhpreet Singh Upendra Sharma Subodh Kumar Marco Landi Marián Brestič Satwinderjeet Kaur
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title_sort	antioxidant, antiproliferative and apoptosis-inducing efficacy of fractions from <i<cassia fistula</i< l. leaves
callnumber	RM1-950
title_auth	Antioxidant, Antiproliferative and Apoptosis-Inducing Efficacy of Fractions from <i<Cassia fistula</i< L. Leaves
abstract	Cassia fistula L. is a highly admirable traditional medicinal plant used for the treatment of various diseases and disorders. The present study was performed to divulge the antioxidant, antiproliferative, and apoptosis-inducing efficacy of fractions from <i<C. fistula</i< leaves. The hexane (CaLH fraction), chloroform (CaLC fraction), ethyl acetate (CaLE fraction), <i<n</i<-butanol (CaLB fraction), and aqueous (CaLA fraction) were sequentially fractionated from 80% methanolic (CaLM extract) of <i<C. fistula</i< leaves. The CaLE fraction was fractionated using column chromatography to yield a pure compound, which was characterized as Epiafzelechin (CFL1) based on <sup<1</sup<H, <sup<13</sup<C, and DEPT135 NMR. Among these fractions, CaLE and isolated CFL1 fractions exhibited an effective antioxidant potential in Ferric ion reducing power, (2,2’-azino-bis (3-ethylbenzothiazoline -6-sulfonic acid)) cation radical scavenging, and nitric oxide radical scavenging assays. Epiafzelechin was investigated for its antiproliferative effects against MG-63 (osteosarcoma), IMR-32 (neuroblastoma), and PC-3 (prostate adenocarcinoma), and was found to inhibit cell proliferation with a GI<sub<50</sub< value of 8.73, 9.15, and 11.8 μM respectively. MG-63 cells underwent apoptotic cell death on treatment with Epiafzelechin as the cells showed the formation of apoptotic bodies, enhanced reactive oxygen species (ROS) generation, mitochondrial membrane depolarization along with an increase in early apoptotic cell population analyzed using Annexin V-FITC/PI double staining assay. Cells showed cell cycle arrest at the G<sub<0</sub</G<sub<1</sub< phase accompanied by a downregulation in the expression levels of p-Akt (Protein kinase B), p-GSK-3β (Glycogen synthase kinase-3 beta), and Bcl-xl (B-cell lymphoma-extra large) proteins. RT-PCR (Real time-polymerase chain reaction) analysis revealed downregulation in the gene expression level of β-catenin and CDK2 (cyclin-dependent kinases-2) while it upregulated the expression level of caspase-8 and p53 genes in MG-63 cells.
abstractGer	Cassia fistula L. is a highly admirable traditional medicinal plant used for the treatment of various diseases and disorders. The present study was performed to divulge the antioxidant, antiproliferative, and apoptosis-inducing efficacy of fractions from <i<C. fistula</i< leaves. The hexane (CaLH fraction), chloroform (CaLC fraction), ethyl acetate (CaLE fraction), <i<n</i<-butanol (CaLB fraction), and aqueous (CaLA fraction) were sequentially fractionated from 80% methanolic (CaLM extract) of <i<C. fistula</i< leaves. The CaLE fraction was fractionated using column chromatography to yield a pure compound, which was characterized as Epiafzelechin (CFL1) based on <sup<1</sup<H, <sup<13</sup<C, and DEPT135 NMR. Among these fractions, CaLE and isolated CFL1 fractions exhibited an effective antioxidant potential in Ferric ion reducing power, (2,2’-azino-bis (3-ethylbenzothiazoline -6-sulfonic acid)) cation radical scavenging, and nitric oxide radical scavenging assays. Epiafzelechin was investigated for its antiproliferative effects against MG-63 (osteosarcoma), IMR-32 (neuroblastoma), and PC-3 (prostate adenocarcinoma), and was found to inhibit cell proliferation with a GI<sub<50</sub< value of 8.73, 9.15, and 11.8 μM respectively. MG-63 cells underwent apoptotic cell death on treatment with Epiafzelechin as the cells showed the formation of apoptotic bodies, enhanced reactive oxygen species (ROS) generation, mitochondrial membrane depolarization along with an increase in early apoptotic cell population analyzed using Annexin V-FITC/PI double staining assay. Cells showed cell cycle arrest at the G<sub<0</sub</G<sub<1</sub< phase accompanied by a downregulation in the expression levels of p-Akt (Protein kinase B), p-GSK-3β (Glycogen synthase kinase-3 beta), and Bcl-xl (B-cell lymphoma-extra large) proteins. RT-PCR (Real time-polymerase chain reaction) analysis revealed downregulation in the gene expression level of β-catenin and CDK2 (cyclin-dependent kinases-2) while it upregulated the expression level of caspase-8 and p53 genes in MG-63 cells.
abstract_unstemmed	Cassia fistula L. is a highly admirable traditional medicinal plant used for the treatment of various diseases and disorders. The present study was performed to divulge the antioxidant, antiproliferative, and apoptosis-inducing efficacy of fractions from <i<C. fistula</i< leaves. The hexane (CaLH fraction), chloroform (CaLC fraction), ethyl acetate (CaLE fraction), <i<n</i<-butanol (CaLB fraction), and aqueous (CaLA fraction) were sequentially fractionated from 80% methanolic (CaLM extract) of <i<C. fistula</i< leaves. The CaLE fraction was fractionated using column chromatography to yield a pure compound, which was characterized as Epiafzelechin (CFL1) based on <sup<1</sup<H, <sup<13</sup<C, and DEPT135 NMR. Among these fractions, CaLE and isolated CFL1 fractions exhibited an effective antioxidant potential in Ferric ion reducing power, (2,2’-azino-bis (3-ethylbenzothiazoline -6-sulfonic acid)) cation radical scavenging, and nitric oxide radical scavenging assays. Epiafzelechin was investigated for its antiproliferative effects against MG-63 (osteosarcoma), IMR-32 (neuroblastoma), and PC-3 (prostate adenocarcinoma), and was found to inhibit cell proliferation with a GI<sub<50</sub< value of 8.73, 9.15, and 11.8 μM respectively. MG-63 cells underwent apoptotic cell death on treatment with Epiafzelechin as the cells showed the formation of apoptotic bodies, enhanced reactive oxygen species (ROS) generation, mitochondrial membrane depolarization along with an increase in early apoptotic cell population analyzed using Annexin V-FITC/PI double staining assay. Cells showed cell cycle arrest at the G<sub<0</sub</G<sub<1</sub< phase accompanied by a downregulation in the expression levels of p-Akt (Protein kinase B), p-GSK-3β (Glycogen synthase kinase-3 beta), and Bcl-xl (B-cell lymphoma-extra large) proteins. RT-PCR (Real time-polymerase chain reaction) analysis revealed downregulation in the gene expression level of β-catenin and CDK2 (cyclin-dependent kinases-2) while it upregulated the expression level of caspase-8 and p53 genes in MG-63 cells.
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container_issue	2, p 173
title_short	Antioxidant, Antiproliferative and Apoptosis-Inducing Efficacy of Fractions from <i<Cassia fistula</i< L. Leaves
url	https://doi.org/10.3390/antiox9020173 https://doaj.org/article/de74e498cf484b28a636fdcab59f46e2 https://www.mdpi.com/2076-3921/9/2/173 https://doaj.org/toc/2076-3921
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Antioxidant, Antiproliferative and Apoptosis-Inducing Efficacy of Fractions from <i<Cassia fistula</i< L. Leaves

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