Molecular Characterization and the Function of Argonaute3 in RNAi Pathway of Plutella xylostella

Argonaute (Ago) protein family plays a key role in the RNA interference (RNAi) process in different insects including Lepidopteran. However, the role of Ago proteins in the RNAi pathway of Plutella xylostella is still unknown. We cloned an Argonaute3 gene in P. xylostella (PxAgo3) with the complete...
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Autor*in:	Muhammad Salman Hameed [verfasserIn] Zhengbing Wang [verfasserIn] Liette Vasseur [verfasserIn] Guang Yang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	lepidopteran pest PxAgo3 phylogenetic tree dsRNA RNAi process

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 19(2018), 4, p 1249
Übergeordnetes Werk:	volume:19 ; year:2018 ; number:4, p 1249

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/ijms19041249

Katalog-ID:	DOAJ04540111X

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520			\|a Argonaute (Ago) protein family plays a key role in the RNA interference (RNAi) process in different insects including Lepidopteran. However, the role of Ago proteins in the RNAi pathway of Plutella xylostella is still unknown. We cloned an Argonaute3 gene in P. xylostella (PxAgo3) with the complete coding sequence of 2832 bp. The encoded protein had 935 amino acids with an expected molecular weight of 108.9 kDa and an isoelectric point of 9.29. It contained a PAZ (PIWI/Argonaute/Zwile) domain and PIWI (P-element-induced whimpy testes) domain. PxAgo3 was classified into the Piwi subfamily of Ago proteins with a high similarity of 93.0% with Bombyx mori Ago3 (BmAgo3). The suppression of PxAgo3 by dsPxAgo3 was observed 3 h after treatment and was maintained until 24 h. Knockdown of PxAgo3 decreased the suppression level of PxActin by dsPxActin in P. xylostella cells, while overexpression of PxAgo3 increased the RNAi efficiency. Our results suggest that PxAgo3 play a key role in the double stranded RNA (dsRNA)-regulated RNAi pathway in P. xylostella.
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allfields	10.3390/ijms19041249 doi (DE-627)DOAJ04540111X (DE-599)DOAJ0adbc287744e408f81abb042265a4169 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Muhammad Salman Hameed verfasserin aut Molecular Characterization and the Function of Argonaute3 in RNAi Pathway of Plutella xylostella 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Argonaute (Ago) protein family plays a key role in the RNA interference (RNAi) process in different insects including Lepidopteran. However, the role of Ago proteins in the RNAi pathway of Plutella xylostella is still unknown. We cloned an Argonaute3 gene in P. xylostella (PxAgo3) with the complete coding sequence of 2832 bp. The encoded protein had 935 amino acids with an expected molecular weight of 108.9 kDa and an isoelectric point of 9.29. It contained a PAZ (PIWI/Argonaute/Zwile) domain and PIWI (P-element-induced whimpy testes) domain. PxAgo3 was classified into the Piwi subfamily of Ago proteins with a high similarity of 93.0% with Bombyx mori Ago3 (BmAgo3). The suppression of PxAgo3 by dsPxAgo3 was observed 3 h after treatment and was maintained until 24 h. Knockdown of PxAgo3 decreased the suppression level of PxActin by dsPxActin in P. xylostella cells, while overexpression of PxAgo3 increased the RNAi efficiency. Our results suggest that PxAgo3 play a key role in the double stranded RNA (dsRNA)-regulated RNAi pathway in P. xylostella. lepidopteran pest PxAgo3 phylogenetic tree dsRNA RNAi process Biology (General) Chemistry Zhengbing Wang verfasserin aut Liette Vasseur verfasserin aut Guang Yang verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 19(2018), 4, p 1249 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:19 year:2018 number:4, p 1249 https://doi.org/10.3390/ijms19041249 kostenfrei https://doaj.org/article/0adbc287744e408f81abb042265a4169 kostenfrei http://www.mdpi.com/1422-0067/19/4/1249 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2018 4, p 1249
spelling	10.3390/ijms19041249 doi (DE-627)DOAJ04540111X (DE-599)DOAJ0adbc287744e408f81abb042265a4169 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Muhammad Salman Hameed verfasserin aut Molecular Characterization and the Function of Argonaute3 in RNAi Pathway of Plutella xylostella 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Argonaute (Ago) protein family plays a key role in the RNA interference (RNAi) process in different insects including Lepidopteran. However, the role of Ago proteins in the RNAi pathway of Plutella xylostella is still unknown. We cloned an Argonaute3 gene in P. xylostella (PxAgo3) with the complete coding sequence of 2832 bp. The encoded protein had 935 amino acids with an expected molecular weight of 108.9 kDa and an isoelectric point of 9.29. It contained a PAZ (PIWI/Argonaute/Zwile) domain and PIWI (P-element-induced whimpy testes) domain. PxAgo3 was classified into the Piwi subfamily of Ago proteins with a high similarity of 93.0% with Bombyx mori Ago3 (BmAgo3). The suppression of PxAgo3 by dsPxAgo3 was observed 3 h after treatment and was maintained until 24 h. Knockdown of PxAgo3 decreased the suppression level of PxActin by dsPxActin in P. xylostella cells, while overexpression of PxAgo3 increased the RNAi efficiency. Our results suggest that PxAgo3 play a key role in the double stranded RNA (dsRNA)-regulated RNAi pathway in P. xylostella. lepidopteran pest PxAgo3 phylogenetic tree dsRNA RNAi process Biology (General) Chemistry Zhengbing Wang verfasserin aut Liette Vasseur verfasserin aut Guang Yang verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 19(2018), 4, p 1249 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:19 year:2018 number:4, p 1249 https://doi.org/10.3390/ijms19041249 kostenfrei https://doaj.org/article/0adbc287744e408f81abb042265a4169 kostenfrei http://www.mdpi.com/1422-0067/19/4/1249 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2018 4, p 1249
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allfieldsGer	10.3390/ijms19041249 doi (DE-627)DOAJ04540111X (DE-599)DOAJ0adbc287744e408f81abb042265a4169 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Muhammad Salman Hameed verfasserin aut Molecular Characterization and the Function of Argonaute3 in RNAi Pathway of Plutella xylostella 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Argonaute (Ago) protein family plays a key role in the RNA interference (RNAi) process in different insects including Lepidopteran. However, the role of Ago proteins in the RNAi pathway of Plutella xylostella is still unknown. We cloned an Argonaute3 gene in P. xylostella (PxAgo3) with the complete coding sequence of 2832 bp. The encoded protein had 935 amino acids with an expected molecular weight of 108.9 kDa and an isoelectric point of 9.29. It contained a PAZ (PIWI/Argonaute/Zwile) domain and PIWI (P-element-induced whimpy testes) domain. PxAgo3 was classified into the Piwi subfamily of Ago proteins with a high similarity of 93.0% with Bombyx mori Ago3 (BmAgo3). The suppression of PxAgo3 by dsPxAgo3 was observed 3 h after treatment and was maintained until 24 h. Knockdown of PxAgo3 decreased the suppression level of PxActin by dsPxActin in P. xylostella cells, while overexpression of PxAgo3 increased the RNAi efficiency. Our results suggest that PxAgo3 play a key role in the double stranded RNA (dsRNA)-regulated RNAi pathway in P. xylostella. lepidopteran pest PxAgo3 phylogenetic tree dsRNA RNAi process Biology (General) Chemistry Zhengbing Wang verfasserin aut Liette Vasseur verfasserin aut Guang Yang verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 19(2018), 4, p 1249 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:19 year:2018 number:4, p 1249 https://doi.org/10.3390/ijms19041249 kostenfrei https://doaj.org/article/0adbc287744e408f81abb042265a4169 kostenfrei http://www.mdpi.com/1422-0067/19/4/1249 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2018 4, p 1249
allfieldsSound	10.3390/ijms19041249 doi (DE-627)DOAJ04540111X (DE-599)DOAJ0adbc287744e408f81abb042265a4169 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Muhammad Salman Hameed verfasserin aut Molecular Characterization and the Function of Argonaute3 in RNAi Pathway of Plutella xylostella 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Argonaute (Ago) protein family plays a key role in the RNA interference (RNAi) process in different insects including Lepidopteran. However, the role of Ago proteins in the RNAi pathway of Plutella xylostella is still unknown. We cloned an Argonaute3 gene in P. xylostella (PxAgo3) with the complete coding sequence of 2832 bp. The encoded protein had 935 amino acids with an expected molecular weight of 108.9 kDa and an isoelectric point of 9.29. It contained a PAZ (PIWI/Argonaute/Zwile) domain and PIWI (P-element-induced whimpy testes) domain. PxAgo3 was classified into the Piwi subfamily of Ago proteins with a high similarity of 93.0% with Bombyx mori Ago3 (BmAgo3). The suppression of PxAgo3 by dsPxAgo3 was observed 3 h after treatment and was maintained until 24 h. Knockdown of PxAgo3 decreased the suppression level of PxActin by dsPxActin in P. xylostella cells, while overexpression of PxAgo3 increased the RNAi efficiency. Our results suggest that PxAgo3 play a key role in the double stranded RNA (dsRNA)-regulated RNAi pathway in P. xylostella. lepidopteran pest PxAgo3 phylogenetic tree dsRNA RNAi process Biology (General) Chemistry Zhengbing Wang verfasserin aut Liette Vasseur verfasserin aut Guang Yang verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 19(2018), 4, p 1249 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:19 year:2018 number:4, p 1249 https://doi.org/10.3390/ijms19041249 kostenfrei https://doaj.org/article/0adbc287744e408f81abb042265a4169 kostenfrei http://www.mdpi.com/1422-0067/19/4/1249 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 19 2018 4, p 1249
language	English
source	In International Journal of Molecular Sciences 19(2018), 4, p 1249 volume:19 year:2018 number:4, p 1249
sourceStr	In International Journal of Molecular Sciences 19(2018), 4, p 1249 volume:19 year:2018 number:4, p 1249
format_phy_str_mv	Article
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topic_facet	lepidopteran pest PxAgo3 phylogenetic tree dsRNA RNAi process Biology (General) Chemistry
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container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Muhammad Salman Hameed @@aut@@ Zhengbing Wang @@aut@@ Liette Vasseur @@aut@@ Guang Yang @@aut@@
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callnumber-first	Q - Science
author	Muhammad Salman Hameed
spellingShingle	Muhammad Salman Hameed misc QH301-705.5 misc QD1-999 misc lepidopteran pest misc PxAgo3 misc phylogenetic tree misc dsRNA misc RNAi process misc Biology (General) misc Chemistry Molecular Characterization and the Function of Argonaute3 in RNAi Pathway of Plutella xylostella
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topic_title	QH301-705.5 QD1-999 Molecular Characterization and the Function of Argonaute3 in RNAi Pathway of Plutella xylostella lepidopteran pest PxAgo3 phylogenetic tree dsRNA RNAi process
topic	misc QH301-705.5 misc QD1-999 misc lepidopteran pest misc PxAgo3 misc phylogenetic tree misc dsRNA misc RNAi process misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc lepidopteran pest misc PxAgo3 misc phylogenetic tree misc dsRNA misc RNAi process misc Biology (General) misc Chemistry
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title	Molecular Characterization and the Function of Argonaute3 in RNAi Pathway of Plutella xylostella
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title_full	Molecular Characterization and the Function of Argonaute3 in RNAi Pathway of Plutella xylostella
author_sort	Muhammad Salman Hameed
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author_browse	Muhammad Salman Hameed Zhengbing Wang Liette Vasseur Guang Yang
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title_sort	molecular characterization and the function of argonaute3 in rnai pathway of plutella xylostella
callnumber	QH301-705.5
title_auth	Molecular Characterization and the Function of Argonaute3 in RNAi Pathway of Plutella xylostella
abstract	Argonaute (Ago) protein family plays a key role in the RNA interference (RNAi) process in different insects including Lepidopteran. However, the role of Ago proteins in the RNAi pathway of Plutella xylostella is still unknown. We cloned an Argonaute3 gene in P. xylostella (PxAgo3) with the complete coding sequence of 2832 bp. The encoded protein had 935 amino acids with an expected molecular weight of 108.9 kDa and an isoelectric point of 9.29. It contained a PAZ (PIWI/Argonaute/Zwile) domain and PIWI (P-element-induced whimpy testes) domain. PxAgo3 was classified into the Piwi subfamily of Ago proteins with a high similarity of 93.0% with Bombyx mori Ago3 (BmAgo3). The suppression of PxAgo3 by dsPxAgo3 was observed 3 h after treatment and was maintained until 24 h. Knockdown of PxAgo3 decreased the suppression level of PxActin by dsPxActin in P. xylostella cells, while overexpression of PxAgo3 increased the RNAi efficiency. Our results suggest that PxAgo3 play a key role in the double stranded RNA (dsRNA)-regulated RNAi pathway in P. xylostella.
abstractGer	Argonaute (Ago) protein family plays a key role in the RNA interference (RNAi) process in different insects including Lepidopteran. However, the role of Ago proteins in the RNAi pathway of Plutella xylostella is still unknown. We cloned an Argonaute3 gene in P. xylostella (PxAgo3) with the complete coding sequence of 2832 bp. The encoded protein had 935 amino acids with an expected molecular weight of 108.9 kDa and an isoelectric point of 9.29. It contained a PAZ (PIWI/Argonaute/Zwile) domain and PIWI (P-element-induced whimpy testes) domain. PxAgo3 was classified into the Piwi subfamily of Ago proteins with a high similarity of 93.0% with Bombyx mori Ago3 (BmAgo3). The suppression of PxAgo3 by dsPxAgo3 was observed 3 h after treatment and was maintained until 24 h. Knockdown of PxAgo3 decreased the suppression level of PxActin by dsPxActin in P. xylostella cells, while overexpression of PxAgo3 increased the RNAi efficiency. Our results suggest that PxAgo3 play a key role in the double stranded RNA (dsRNA)-regulated RNAi pathway in P. xylostella.
abstract_unstemmed	Argonaute (Ago) protein family plays a key role in the RNA interference (RNAi) process in different insects including Lepidopteran. However, the role of Ago proteins in the RNAi pathway of Plutella xylostella is still unknown. We cloned an Argonaute3 gene in P. xylostella (PxAgo3) with the complete coding sequence of 2832 bp. The encoded protein had 935 amino acids with an expected molecular weight of 108.9 kDa and an isoelectric point of 9.29. It contained a PAZ (PIWI/Argonaute/Zwile) domain and PIWI (P-element-induced whimpy testes) domain. PxAgo3 was classified into the Piwi subfamily of Ago proteins with a high similarity of 93.0% with Bombyx mori Ago3 (BmAgo3). The suppression of PxAgo3 by dsPxAgo3 was observed 3 h after treatment and was maintained until 24 h. Knockdown of PxAgo3 decreased the suppression level of PxActin by dsPxActin in P. xylostella cells, while overexpression of PxAgo3 increased the RNAi efficiency. Our results suggest that PxAgo3 play a key role in the double stranded RNA (dsRNA)-regulated RNAi pathway in P. xylostella.
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container_issue	4, p 1249
title_short	Molecular Characterization and the Function of Argonaute3 in RNAi Pathway of Plutella xylostella
url	https://doi.org/10.3390/ijms19041249 https://doaj.org/article/0adbc287744e408f81abb042265a4169 http://www.mdpi.com/1422-0067/19/4/1249 https://doaj.org/toc/1422-0067
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Molecular Characterization and the Function of Argonaute3 in RNAi Pathway of Plutella xylostella

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