Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion‐exchange method

Abstract The development of a new large‐scale purification protocol is required for research on the reliable bioactivity and drug discovery of extracellular vesicles (EVs). To address this issue, herein, we propose an effective method for preparing high‐performance exosomes (EXOs) by using an anion‐...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Naohiro Seo [verfasserIn] Junko Nakamura [verfasserIn] Tsuguhiro Kaneda [verfasserIn] Hiroaki Tateno [verfasserIn] Asako Shimoda [verfasserIn] Takanori Ichiki [verfasserIn] Koichi Furukawa [verfasserIn] Jun Hirabayashi [verfasserIn] Kazunari Akiyoshi [verfasserIn] Hiroshi Shiku [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	anion‐exchange exosome extracellular vesicle large‐preparation membrane charge microvesicle

Übergeordnetes Werk:	In: Journal of Extracellular Vesicles - Wiley, 2012, 11(2022), 3, Seite n/a-n/a
Übergeordnetes Werk:	volume:11 ; year:2022 ; number:3 ; pages:n/a-n/a

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1002/jev2.12205

Katalog-ID:	DOAJ045488185

Internformat


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245	1	0	\|a Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion‐exchange method
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520			\|a Abstract The development of a new large‐scale purification protocol is required for research on the reliable bioactivity and drug discovery of extracellular vesicles (EVs). To address this issue, herein, we propose an effective method for preparing high‐performance exosomes (EXOs) by using an anion‐exchange method. Cytotoxic T‐lymphocyte (CTL) EVs from 4 L of culture supernatant through a 220 nm cut‐off filter are divided into two populations at a deproteinization rate of over 99.97%, which are eluted at low (0.15 M–0.3 M) and high (0.3 M–0.5 M) NaCl concentrations (approximately 2 × 1012 and 1.5 × 1012 particles, respectively) through the anion‐exchange column chromatography. The former are abundant in EXO proteins, including late endosome‐associated proteins and rab‐family and integrin‐family proteins, and functional micro (mi) RNAs, and have bioactivity for preventing tumour metastasis by depleting mesenchymal cell populations in the primary tumour lesions. By contrast, the latter is microvesicle (MV)‐like particles including DNA, core histone and ribosomal proteins, and GC‐rich miRNAs with unknown function, and are easily phagocytosed by mannose receptor+ Kupffer cells. Thus, the anion‐exchange method is suitable for the large‐scale separation of bioactive EXOs and MV‐like EVs as a cargo for dangerous nucleic acids at high‐purity.
650		4	\|a anion‐exchange
650		4	\|a exosome
650		4	\|a extracellular vesicle
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653		0	\|a Cytology
700	0		\|a Junko Nakamura \|e verfasserin \|4 aut
700	0		\|a Tsuguhiro Kaneda \|e verfasserin \|4 aut
700	0		\|a Hiroaki Tateno \|e verfasserin \|4 aut
700	0		\|a Asako Shimoda \|e verfasserin \|4 aut
700	0		\|a Takanori Ichiki \|e verfasserin \|4 aut
700	0		\|a Koichi Furukawa \|e verfasserin \|4 aut
700	0		\|a Jun Hirabayashi \|e verfasserin \|4 aut
700	0		\|a Kazunari Akiyoshi \|e verfasserin \|4 aut
700	0		\|a Hiroshi Shiku \|e verfasserin \|4 aut
773	0	8	\|i In \|t Journal of Extracellular Vesicles \|d Wiley, 2012 \|g 11(2022), 3, Seite n/a-n/a \|w (DE-627)726923710 \|w (DE-600)2683797-3 \|x 20013078 \|7 nnns
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856	4	2	\|u https://doaj.org/toc/2001-3078 \|y Journal toc \|z kostenfrei
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912			\|a GBV_ILN_31
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_95
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_151
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_171
912			\|a GBV_ILN_206
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_602
912			\|a GBV_ILN_636
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2007
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2037
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2056
912			\|a GBV_ILN_2057
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2088
912			\|a GBV_ILN_2106
912			\|a GBV_ILN_2108
912			\|a GBV_ILN_2110
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2122
912			\|a GBV_ILN_2143
912			\|a GBV_ILN_2144
912			\|a GBV_ILN_2147
912			\|a GBV_ILN_2148
912			\|a GBV_ILN_2152
912			\|a GBV_ILN_2153
912			\|a GBV_ILN_2232
912			\|a GBV_ILN_2336
912			\|a GBV_ILN_2470
912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4046
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4336
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4700
951			\|a AR
952			\|d 11 \|j 2022 \|e 3 \|h n/a-n/a

Indexfelder

author_variant	n s ns j n jn t k tk h t ht a s as t i ti k f kf j h jh k a ka h s hs
matchkey_str	article:20013078:2022----::itnusigucinlxsmsnohrxrcluavsceaauliaicr
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publishDate	2022
allfields	10.1002/jev2.12205 doi (DE-627)DOAJ045488185 (DE-599)DOAJec048ebd4cd04199bbc9a4fcae2aff61 DE-627 ger DE-627 rakwb eng QH573-671 Naohiro Seo verfasserin aut Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion‐exchange method 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The development of a new large‐scale purification protocol is required for research on the reliable bioactivity and drug discovery of extracellular vesicles (EVs). To address this issue, herein, we propose an effective method for preparing high‐performance exosomes (EXOs) by using an anion‐exchange method. Cytotoxic T‐lymphocyte (CTL) EVs from 4 L of culture supernatant through a 220 nm cut‐off filter are divided into two populations at a deproteinization rate of over 99.97%, which are eluted at low (0.15 M–0.3 M) and high (0.3 M–0.5 M) NaCl concentrations (approximately 2 × 1012 and 1.5 × 1012 particles, respectively) through the anion‐exchange column chromatography. The former are abundant in EXO proteins, including late endosome‐associated proteins and rab‐family and integrin‐family proteins, and functional micro (mi) RNAs, and have bioactivity for preventing tumour metastasis by depleting mesenchymal cell populations in the primary tumour lesions. By contrast, the latter is microvesicle (MV)‐like particles including DNA, core histone and ribosomal proteins, and GC‐rich miRNAs with unknown function, and are easily phagocytosed by mannose receptor+ Kupffer cells. Thus, the anion‐exchange method is suitable for the large‐scale separation of bioactive EXOs and MV‐like EVs as a cargo for dangerous nucleic acids at high‐purity. anion‐exchange exosome extracellular vesicle large‐preparation membrane charge microvesicle Cytology Junko Nakamura verfasserin aut Tsuguhiro Kaneda verfasserin aut Hiroaki Tateno verfasserin aut Asako Shimoda verfasserin aut Takanori Ichiki verfasserin aut Koichi Furukawa verfasserin aut Jun Hirabayashi verfasserin aut Kazunari Akiyoshi verfasserin aut Hiroshi Shiku verfasserin aut In Journal of Extracellular Vesicles Wiley, 2012 11(2022), 3, Seite n/a-n/a (DE-627)726923710 (DE-600)2683797-3 20013078 nnns volume:11 year:2022 number:3 pages:n/a-n/a https://doi.org/10.1002/jev2.12205 kostenfrei https://doaj.org/article/ec048ebd4cd04199bbc9a4fcae2aff61 kostenfrei https://doi.org/10.1002/jev2.12205 kostenfrei https://doaj.org/toc/2001-3078 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 3 n/a-n/a
spelling	10.1002/jev2.12205 doi (DE-627)DOAJ045488185 (DE-599)DOAJec048ebd4cd04199bbc9a4fcae2aff61 DE-627 ger DE-627 rakwb eng QH573-671 Naohiro Seo verfasserin aut Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion‐exchange method 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The development of a new large‐scale purification protocol is required for research on the reliable bioactivity and drug discovery of extracellular vesicles (EVs). To address this issue, herein, we propose an effective method for preparing high‐performance exosomes (EXOs) by using an anion‐exchange method. Cytotoxic T‐lymphocyte (CTL) EVs from 4 L of culture supernatant through a 220 nm cut‐off filter are divided into two populations at a deproteinization rate of over 99.97%, which are eluted at low (0.15 M–0.3 M) and high (0.3 M–0.5 M) NaCl concentrations (approximately 2 × 1012 and 1.5 × 1012 particles, respectively) through the anion‐exchange column chromatography. The former are abundant in EXO proteins, including late endosome‐associated proteins and rab‐family and integrin‐family proteins, and functional micro (mi) RNAs, and have bioactivity for preventing tumour metastasis by depleting mesenchymal cell populations in the primary tumour lesions. By contrast, the latter is microvesicle (MV)‐like particles including DNA, core histone and ribosomal proteins, and GC‐rich miRNAs with unknown function, and are easily phagocytosed by mannose receptor+ Kupffer cells. Thus, the anion‐exchange method is suitable for the large‐scale separation of bioactive EXOs and MV‐like EVs as a cargo for dangerous nucleic acids at high‐purity. anion‐exchange exosome extracellular vesicle large‐preparation membrane charge microvesicle Cytology Junko Nakamura verfasserin aut Tsuguhiro Kaneda verfasserin aut Hiroaki Tateno verfasserin aut Asako Shimoda verfasserin aut Takanori Ichiki verfasserin aut Koichi Furukawa verfasserin aut Jun Hirabayashi verfasserin aut Kazunari Akiyoshi verfasserin aut Hiroshi Shiku verfasserin aut In Journal of Extracellular Vesicles Wiley, 2012 11(2022), 3, Seite n/a-n/a (DE-627)726923710 (DE-600)2683797-3 20013078 nnns volume:11 year:2022 number:3 pages:n/a-n/a https://doi.org/10.1002/jev2.12205 kostenfrei https://doaj.org/article/ec048ebd4cd04199bbc9a4fcae2aff61 kostenfrei https://doi.org/10.1002/jev2.12205 kostenfrei https://doaj.org/toc/2001-3078 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 3 n/a-n/a
allfields_unstemmed	10.1002/jev2.12205 doi (DE-627)DOAJ045488185 (DE-599)DOAJec048ebd4cd04199bbc9a4fcae2aff61 DE-627 ger DE-627 rakwb eng QH573-671 Naohiro Seo verfasserin aut Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion‐exchange method 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The development of a new large‐scale purification protocol is required for research on the reliable bioactivity and drug discovery of extracellular vesicles (EVs). To address this issue, herein, we propose an effective method for preparing high‐performance exosomes (EXOs) by using an anion‐exchange method. Cytotoxic T‐lymphocyte (CTL) EVs from 4 L of culture supernatant through a 220 nm cut‐off filter are divided into two populations at a deproteinization rate of over 99.97%, which are eluted at low (0.15 M–0.3 M) and high (0.3 M–0.5 M) NaCl concentrations (approximately 2 × 1012 and 1.5 × 1012 particles, respectively) through the anion‐exchange column chromatography. The former are abundant in EXO proteins, including late endosome‐associated proteins and rab‐family and integrin‐family proteins, and functional micro (mi) RNAs, and have bioactivity for preventing tumour metastasis by depleting mesenchymal cell populations in the primary tumour lesions. By contrast, the latter is microvesicle (MV)‐like particles including DNA, core histone and ribosomal proteins, and GC‐rich miRNAs with unknown function, and are easily phagocytosed by mannose receptor+ Kupffer cells. Thus, the anion‐exchange method is suitable for the large‐scale separation of bioactive EXOs and MV‐like EVs as a cargo for dangerous nucleic acids at high‐purity. anion‐exchange exosome extracellular vesicle large‐preparation membrane charge microvesicle Cytology Junko Nakamura verfasserin aut Tsuguhiro Kaneda verfasserin aut Hiroaki Tateno verfasserin aut Asako Shimoda verfasserin aut Takanori Ichiki verfasserin aut Koichi Furukawa verfasserin aut Jun Hirabayashi verfasserin aut Kazunari Akiyoshi verfasserin aut Hiroshi Shiku verfasserin aut In Journal of Extracellular Vesicles Wiley, 2012 11(2022), 3, Seite n/a-n/a (DE-627)726923710 (DE-600)2683797-3 20013078 nnns volume:11 year:2022 number:3 pages:n/a-n/a https://doi.org/10.1002/jev2.12205 kostenfrei https://doaj.org/article/ec048ebd4cd04199bbc9a4fcae2aff61 kostenfrei https://doi.org/10.1002/jev2.12205 kostenfrei https://doaj.org/toc/2001-3078 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 3 n/a-n/a
allfieldsGer	10.1002/jev2.12205 doi (DE-627)DOAJ045488185 (DE-599)DOAJec048ebd4cd04199bbc9a4fcae2aff61 DE-627 ger DE-627 rakwb eng QH573-671 Naohiro Seo verfasserin aut Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion‐exchange method 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The development of a new large‐scale purification protocol is required for research on the reliable bioactivity and drug discovery of extracellular vesicles (EVs). To address this issue, herein, we propose an effective method for preparing high‐performance exosomes (EXOs) by using an anion‐exchange method. Cytotoxic T‐lymphocyte (CTL) EVs from 4 L of culture supernatant through a 220 nm cut‐off filter are divided into two populations at a deproteinization rate of over 99.97%, which are eluted at low (0.15 M–0.3 M) and high (0.3 M–0.5 M) NaCl concentrations (approximately 2 × 1012 and 1.5 × 1012 particles, respectively) through the anion‐exchange column chromatography. The former are abundant in EXO proteins, including late endosome‐associated proteins and rab‐family and integrin‐family proteins, and functional micro (mi) RNAs, and have bioactivity for preventing tumour metastasis by depleting mesenchymal cell populations in the primary tumour lesions. By contrast, the latter is microvesicle (MV)‐like particles including DNA, core histone and ribosomal proteins, and GC‐rich miRNAs with unknown function, and are easily phagocytosed by mannose receptor+ Kupffer cells. Thus, the anion‐exchange method is suitable for the large‐scale separation of bioactive EXOs and MV‐like EVs as a cargo for dangerous nucleic acids at high‐purity. anion‐exchange exosome extracellular vesicle large‐preparation membrane charge microvesicle Cytology Junko Nakamura verfasserin aut Tsuguhiro Kaneda verfasserin aut Hiroaki Tateno verfasserin aut Asako Shimoda verfasserin aut Takanori Ichiki verfasserin aut Koichi Furukawa verfasserin aut Jun Hirabayashi verfasserin aut Kazunari Akiyoshi verfasserin aut Hiroshi Shiku verfasserin aut In Journal of Extracellular Vesicles Wiley, 2012 11(2022), 3, Seite n/a-n/a (DE-627)726923710 (DE-600)2683797-3 20013078 nnns volume:11 year:2022 number:3 pages:n/a-n/a https://doi.org/10.1002/jev2.12205 kostenfrei https://doaj.org/article/ec048ebd4cd04199bbc9a4fcae2aff61 kostenfrei https://doi.org/10.1002/jev2.12205 kostenfrei https://doaj.org/toc/2001-3078 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 3 n/a-n/a
allfieldsSound	10.1002/jev2.12205 doi (DE-627)DOAJ045488185 (DE-599)DOAJec048ebd4cd04199bbc9a4fcae2aff61 DE-627 ger DE-627 rakwb eng QH573-671 Naohiro Seo verfasserin aut Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion‐exchange method 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract The development of a new large‐scale purification protocol is required for research on the reliable bioactivity and drug discovery of extracellular vesicles (EVs). To address this issue, herein, we propose an effective method for preparing high‐performance exosomes (EXOs) by using an anion‐exchange method. Cytotoxic T‐lymphocyte (CTL) EVs from 4 L of culture supernatant through a 220 nm cut‐off filter are divided into two populations at a deproteinization rate of over 99.97%, which are eluted at low (0.15 M–0.3 M) and high (0.3 M–0.5 M) NaCl concentrations (approximately 2 × 1012 and 1.5 × 1012 particles, respectively) through the anion‐exchange column chromatography. The former are abundant in EXO proteins, including late endosome‐associated proteins and rab‐family and integrin‐family proteins, and functional micro (mi) RNAs, and have bioactivity for preventing tumour metastasis by depleting mesenchymal cell populations in the primary tumour lesions. By contrast, the latter is microvesicle (MV)‐like particles including DNA, core histone and ribosomal proteins, and GC‐rich miRNAs with unknown function, and are easily phagocytosed by mannose receptor+ Kupffer cells. Thus, the anion‐exchange method is suitable for the large‐scale separation of bioactive EXOs and MV‐like EVs as a cargo for dangerous nucleic acids at high‐purity. anion‐exchange exosome extracellular vesicle large‐preparation membrane charge microvesicle Cytology Junko Nakamura verfasserin aut Tsuguhiro Kaneda verfasserin aut Hiroaki Tateno verfasserin aut Asako Shimoda verfasserin aut Takanori Ichiki verfasserin aut Koichi Furukawa verfasserin aut Jun Hirabayashi verfasserin aut Kazunari Akiyoshi verfasserin aut Hiroshi Shiku verfasserin aut In Journal of Extracellular Vesicles Wiley, 2012 11(2022), 3, Seite n/a-n/a (DE-627)726923710 (DE-600)2683797-3 20013078 nnns volume:11 year:2022 number:3 pages:n/a-n/a https://doi.org/10.1002/jev2.12205 kostenfrei https://doaj.org/article/ec048ebd4cd04199bbc9a4fcae2aff61 kostenfrei https://doi.org/10.1002/jev2.12205 kostenfrei https://doaj.org/toc/2001-3078 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2022 3 n/a-n/a
language	English
source	In Journal of Extracellular Vesicles 11(2022), 3, Seite n/a-n/a volume:11 year:2022 number:3 pages:n/a-n/a
sourceStr	In Journal of Extracellular Vesicles 11(2022), 3, Seite n/a-n/a volume:11 year:2022 number:3 pages:n/a-n/a
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	anion‐exchange exosome extracellular vesicle large‐preparation membrane charge microvesicle Cytology
isfreeaccess_bool	true
container_title	Journal of Extracellular Vesicles
authorswithroles_txt_mv	Naohiro Seo @@aut@@ Junko Nakamura @@aut@@ Tsuguhiro Kaneda @@aut@@ Hiroaki Tateno @@aut@@ Asako Shimoda @@aut@@ Takanori Ichiki @@aut@@ Koichi Furukawa @@aut@@ Jun Hirabayashi @@aut@@ Kazunari Akiyoshi @@aut@@ Hiroshi Shiku @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	726923710
id	DOAJ045488185
language_de	englisch
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callnumber-first	Q - Science
author	Naohiro Seo
spellingShingle	Naohiro Seo misc QH573-671 misc anion‐exchange misc exosome misc extracellular vesicle misc large‐preparation misc membrane charge misc microvesicle misc Cytology Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion‐exchange method
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topic_title	QH573-671 Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion‐exchange method anion‐exchange exosome extracellular vesicle large‐preparation membrane charge microvesicle
topic	misc QH573-671 misc anion‐exchange misc exosome misc extracellular vesicle misc large‐preparation misc membrane charge misc microvesicle misc Cytology
topic_unstemmed	misc QH573-671 misc anion‐exchange misc exosome misc extracellular vesicle misc large‐preparation misc membrane charge misc microvesicle misc Cytology
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title	Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion‐exchange method
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title_full	Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion‐exchange method
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author_browse	Naohiro Seo Junko Nakamura Tsuguhiro Kaneda Hiroaki Tateno Asako Shimoda Takanori Ichiki Koichi Furukawa Jun Hirabayashi Kazunari Akiyoshi Hiroshi Shiku
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title_sort	distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion‐exchange method
callnumber	QH573-671
title_auth	Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion‐exchange method
abstract	Abstract The development of a new large‐scale purification protocol is required for research on the reliable bioactivity and drug discovery of extracellular vesicles (EVs). To address this issue, herein, we propose an effective method for preparing high‐performance exosomes (EXOs) by using an anion‐exchange method. Cytotoxic T‐lymphocyte (CTL) EVs from 4 L of culture supernatant through a 220 nm cut‐off filter are divided into two populations at a deproteinization rate of over 99.97%, which are eluted at low (0.15 M–0.3 M) and high (0.3 M–0.5 M) NaCl concentrations (approximately 2 × 1012 and 1.5 × 1012 particles, respectively) through the anion‐exchange column chromatography. The former are abundant in EXO proteins, including late endosome‐associated proteins and rab‐family and integrin‐family proteins, and functional micro (mi) RNAs, and have bioactivity for preventing tumour metastasis by depleting mesenchymal cell populations in the primary tumour lesions. By contrast, the latter is microvesicle (MV)‐like particles including DNA, core histone and ribosomal proteins, and GC‐rich miRNAs with unknown function, and are easily phagocytosed by mannose receptor+ Kupffer cells. Thus, the anion‐exchange method is suitable for the large‐scale separation of bioactive EXOs and MV‐like EVs as a cargo for dangerous nucleic acids at high‐purity.
abstractGer	Abstract The development of a new large‐scale purification protocol is required for research on the reliable bioactivity and drug discovery of extracellular vesicles (EVs). To address this issue, herein, we propose an effective method for preparing high‐performance exosomes (EXOs) by using an anion‐exchange method. Cytotoxic T‐lymphocyte (CTL) EVs from 4 L of culture supernatant through a 220 nm cut‐off filter are divided into two populations at a deproteinization rate of over 99.97%, which are eluted at low (0.15 M–0.3 M) and high (0.3 M–0.5 M) NaCl concentrations (approximately 2 × 1012 and 1.5 × 1012 particles, respectively) through the anion‐exchange column chromatography. The former are abundant in EXO proteins, including late endosome‐associated proteins and rab‐family and integrin‐family proteins, and functional micro (mi) RNAs, and have bioactivity for preventing tumour metastasis by depleting mesenchymal cell populations in the primary tumour lesions. By contrast, the latter is microvesicle (MV)‐like particles including DNA, core histone and ribosomal proteins, and GC‐rich miRNAs with unknown function, and are easily phagocytosed by mannose receptor+ Kupffer cells. Thus, the anion‐exchange method is suitable for the large‐scale separation of bioactive EXOs and MV‐like EVs as a cargo for dangerous nucleic acids at high‐purity.
abstract_unstemmed	Abstract The development of a new large‐scale purification protocol is required for research on the reliable bioactivity and drug discovery of extracellular vesicles (EVs). To address this issue, herein, we propose an effective method for preparing high‐performance exosomes (EXOs) by using an anion‐exchange method. Cytotoxic T‐lymphocyte (CTL) EVs from 4 L of culture supernatant through a 220 nm cut‐off filter are divided into two populations at a deproteinization rate of over 99.97%, which are eluted at low (0.15 M–0.3 M) and high (0.3 M–0.5 M) NaCl concentrations (approximately 2 × 1012 and 1.5 × 1012 particles, respectively) through the anion‐exchange column chromatography. The former are abundant in EXO proteins, including late endosome‐associated proteins and rab‐family and integrin‐family proteins, and functional micro (mi) RNAs, and have bioactivity for preventing tumour metastasis by depleting mesenchymal cell populations in the primary tumour lesions. By contrast, the latter is microvesicle (MV)‐like particles including DNA, core histone and ribosomal proteins, and GC‐rich miRNAs with unknown function, and are easily phagocytosed by mannose receptor+ Kupffer cells. Thus, the anion‐exchange method is suitable for the large‐scale separation of bioactive EXOs and MV‐like EVs as a cargo for dangerous nucleic acids at high‐purity.
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title_short	Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion‐exchange method
url	https://doi.org/10.1002/jev2.12205 https://doaj.org/article/ec048ebd4cd04199bbc9a4fcae2aff61 https://doaj.org/toc/2001-3078
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up_date	2024-07-03T15:14:54.451Z
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fullrecord_marcxml	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ045488185</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230308093003.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230227s2022 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1002/jev2.12205</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ045488185</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJec048ebd4cd04199bbc9a4fcae2aff61</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QH573-671</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Naohiro Seo</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion‐exchange method</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2022</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract The development of a new large‐scale purification protocol is required for research on the reliable bioactivity and drug discovery of extracellular vesicles (EVs). To address this issue, herein, we propose an effective method for preparing high‐performance exosomes (EXOs) by using an anion‐exchange method. Cytotoxic T‐lymphocyte (CTL) EVs from 4 L of culture supernatant through a 220 nm cut‐off filter are divided into two populations at a deproteinization rate of over 99.97%, which are eluted at low (0.15 M–0.3 M) and high (0.3 M–0.5 M) NaCl concentrations (approximately 2 × 1012 and 1.5 × 1012 particles, respectively) through the anion‐exchange column chromatography. The former are abundant in EXO proteins, including late endosome‐associated proteins and rab‐family and integrin‐family proteins, and functional micro (mi) RNAs, and have bioactivity for preventing tumour metastasis by depleting mesenchymal cell populations in the primary tumour lesions. By contrast, the latter is microvesicle (MV)‐like particles including DNA, core histone and ribosomal proteins, and GC‐rich miRNAs with unknown function, and are easily phagocytosed by mannose receptor+ Kupffer cells. Thus, the anion‐exchange method is suitable for the large‐scale separation of bioactive EXOs and MV‐like EVs as a cargo for dangerous nucleic acids at high‐purity.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">anion‐exchange</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">exosome</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">extracellular vesicle</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">large‐preparation</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">membrane charge</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">microvesicle</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Cytology</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Junko Nakamura</subfield><subfield code="e">verfasserin</subfield><subfield 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code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Kazunari Akiyoshi</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Hiroshi Shiku</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Journal of Extracellular Vesicles</subfield><subfield code="d">Wiley, 2012</subfield><subfield code="g">11(2022), 3, Seite n/a-n/a</subfield><subfield code="w">(DE-627)726923710</subfield><subfield code="w">(DE-600)2683797-3</subfield><subfield code="x">20013078</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:11</subfield><subfield code="g">year:2022</subfield><subfield code="g">number:3</subfield><subfield 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Distinguishing functional exosomes and other extracellular vesicles as a nucleic acid cargo by the anion‐exchange method

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