Transcriptome Co-expression Network and Metabolome Analysis Identifies Key Genes and Regulators of Proanthocyanidins Biosynthesis in Brown Cotton

Brown cotton fiber (BCF) is a unique raw material of naturally colored cotton (NCC). But characteristics of the regulatory gene network and metabolic components related to the proanthocyanidins biosynthesis pathway at various stages of its fiber development remain unclear. Here, the dynamic changes...
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Autor*in:	Zhenzhen Wang [verfasserIn] Xiaomeng Zhang [verfasserIn] Shoupu He [verfasserIn] Abdul Rehman [verfasserIn] Yinhua Jia [verfasserIn] Hongge Li [verfasserIn] Zhaoe Pan [verfasserIn] Xiaoli Geng [verfasserIn] Qiong Gao [verfasserIn] Liru Wang [verfasserIn] Zhen Peng [verfasserIn] Xiongming Du [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	brown cotton transcriptome metabolome flavonoid metabolism yeast one-hybrid R2R3-MYB genes

Übergeordnetes Werk:	In: Frontiers in Plant Science - Frontiers Media S.A., 2011, 12(2022)
Übergeordnetes Werk:	volume:12 ; year:2022

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fpls.2021.822198

Katalog-ID:	DOAJ045490481

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allfields	10.3389/fpls.2021.822198 doi (DE-627)DOAJ045490481 (DE-599)DOAJ0623de1413a44319b947537ee055a0d3 DE-627 ger DE-627 rakwb eng SB1-1110 Zhenzhen Wang verfasserin aut Transcriptome Co-expression Network and Metabolome Analysis Identifies Key Genes and Regulators of Proanthocyanidins Biosynthesis in Brown Cotton 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Brown cotton fiber (BCF) is a unique raw material of naturally colored cotton (NCC). But characteristics of the regulatory gene network and metabolic components related to the proanthocyanidins biosynthesis pathway at various stages of its fiber development remain unclear. Here, the dynamic changes in proanthocyanidins biosynthesis components and transcripts in the BCF variety “Zong 1-61” and its white near-isogenic lines (NILs) “RT” were characterized at five fiber developmental stages (0, 5, 10, 15, and 20 days post-anthesis; DPA). Enrichment analysis of differentially expressed genes (DEGs), comparison of metabolome differences, and pathway enrichment analysis of a weighted gene correlation network analysis together revealed the dominant gene expression of flavonoid biosynthesis (FB), phenylpropanoid metabolisms, and some carbohydrate metabolisms at 15 or 20 DPA than white cotton. Eventually, 63 genes were identified from five modules putatively related to FB. Three R2R3-MYB and two bHLH transcription factors were predicted as the core genes. Further, GhANS, GhANR1, and GhUFGT2 were preliminarily regulated by GhMYB46, GhMYB6, and GhMYB3, respectively, according to yeast one-hybrid assays in vitro. Our findings provide an important transcriptional regulatory network of proanthocyanidins biosynthesis pathway and dynamic flavonoid metabolism profiles. brown cotton transcriptome metabolome flavonoid metabolism yeast one-hybrid R2R3-MYB genes Plant culture Xiaomeng Zhang verfasserin aut Shoupu He verfasserin aut Shoupu He verfasserin aut Shoupu He verfasserin aut Abdul Rehman verfasserin aut Yinhua Jia verfasserin aut Hongge Li verfasserin aut Hongge Li verfasserin aut Zhaoe Pan verfasserin aut Xiaoli Geng verfasserin aut Qiong Gao verfasserin aut Liru Wang verfasserin aut Zhen Peng verfasserin aut Zhen Peng verfasserin aut Zhen Peng verfasserin aut Xiongming Du verfasserin aut Xiongming Du verfasserin aut Xiongming Du verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 12(2022) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:12 year:2022 https://doi.org/10.3389/fpls.2021.822198 kostenfrei https://doaj.org/article/0623de1413a44319b947537ee055a0d3 kostenfrei https://www.frontiersin.org/articles/10.3389/fpls.2021.822198/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022
spelling	10.3389/fpls.2021.822198 doi (DE-627)DOAJ045490481 (DE-599)DOAJ0623de1413a44319b947537ee055a0d3 DE-627 ger DE-627 rakwb eng SB1-1110 Zhenzhen Wang verfasserin aut Transcriptome Co-expression Network and Metabolome Analysis Identifies Key Genes and Regulators of Proanthocyanidins Biosynthesis in Brown Cotton 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Brown cotton fiber (BCF) is a unique raw material of naturally colored cotton (NCC). But characteristics of the regulatory gene network and metabolic components related to the proanthocyanidins biosynthesis pathway at various stages of its fiber development remain unclear. Here, the dynamic changes in proanthocyanidins biosynthesis components and transcripts in the BCF variety “Zong 1-61” and its white near-isogenic lines (NILs) “RT” were characterized at five fiber developmental stages (0, 5, 10, 15, and 20 days post-anthesis; DPA). Enrichment analysis of differentially expressed genes (DEGs), comparison of metabolome differences, and pathway enrichment analysis of a weighted gene correlation network analysis together revealed the dominant gene expression of flavonoid biosynthesis (FB), phenylpropanoid metabolisms, and some carbohydrate metabolisms at 15 or 20 DPA than white cotton. Eventually, 63 genes were identified from five modules putatively related to FB. Three R2R3-MYB and two bHLH transcription factors were predicted as the core genes. Further, GhANS, GhANR1, and GhUFGT2 were preliminarily regulated by GhMYB46, GhMYB6, and GhMYB3, respectively, according to yeast one-hybrid assays in vitro. Our findings provide an important transcriptional regulatory network of proanthocyanidins biosynthesis pathway and dynamic flavonoid metabolism profiles. brown cotton transcriptome metabolome flavonoid metabolism yeast one-hybrid R2R3-MYB genes Plant culture Xiaomeng Zhang verfasserin aut Shoupu He verfasserin aut Shoupu He verfasserin aut Shoupu He verfasserin aut Abdul Rehman verfasserin aut Yinhua Jia verfasserin aut Hongge Li verfasserin aut Hongge Li verfasserin aut Zhaoe Pan verfasserin aut Xiaoli Geng verfasserin aut Qiong Gao verfasserin aut Liru Wang verfasserin aut Zhen Peng verfasserin aut Zhen Peng verfasserin aut Zhen Peng verfasserin aut Xiongming Du verfasserin aut Xiongming Du verfasserin aut Xiongming Du verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 12(2022) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:12 year:2022 https://doi.org/10.3389/fpls.2021.822198 kostenfrei https://doaj.org/article/0623de1413a44319b947537ee055a0d3 kostenfrei https://www.frontiersin.org/articles/10.3389/fpls.2021.822198/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022
allfields_unstemmed	10.3389/fpls.2021.822198 doi (DE-627)DOAJ045490481 (DE-599)DOAJ0623de1413a44319b947537ee055a0d3 DE-627 ger DE-627 rakwb eng SB1-1110 Zhenzhen Wang verfasserin aut Transcriptome Co-expression Network and Metabolome Analysis Identifies Key Genes and Regulators of Proanthocyanidins Biosynthesis in Brown Cotton 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Brown cotton fiber (BCF) is a unique raw material of naturally colored cotton (NCC). But characteristics of the regulatory gene network and metabolic components related to the proanthocyanidins biosynthesis pathway at various stages of its fiber development remain unclear. Here, the dynamic changes in proanthocyanidins biosynthesis components and transcripts in the BCF variety “Zong 1-61” and its white near-isogenic lines (NILs) “RT” were characterized at five fiber developmental stages (0, 5, 10, 15, and 20 days post-anthesis; DPA). Enrichment analysis of differentially expressed genes (DEGs), comparison of metabolome differences, and pathway enrichment analysis of a weighted gene correlation network analysis together revealed the dominant gene expression of flavonoid biosynthesis (FB), phenylpropanoid metabolisms, and some carbohydrate metabolisms at 15 or 20 DPA than white cotton. Eventually, 63 genes were identified from five modules putatively related to FB. Three R2R3-MYB and two bHLH transcription factors were predicted as the core genes. Further, GhANS, GhANR1, and GhUFGT2 were preliminarily regulated by GhMYB46, GhMYB6, and GhMYB3, respectively, according to yeast one-hybrid assays in vitro. Our findings provide an important transcriptional regulatory network of proanthocyanidins biosynthesis pathway and dynamic flavonoid metabolism profiles. brown cotton transcriptome metabolome flavonoid metabolism yeast one-hybrid R2R3-MYB genes Plant culture Xiaomeng Zhang verfasserin aut Shoupu He verfasserin aut Shoupu He verfasserin aut Shoupu He verfasserin aut Abdul Rehman verfasserin aut Yinhua Jia verfasserin aut Hongge Li verfasserin aut Hongge Li verfasserin aut Zhaoe Pan verfasserin aut Xiaoli Geng verfasserin aut Qiong Gao verfasserin aut Liru Wang verfasserin aut Zhen Peng verfasserin aut Zhen Peng verfasserin aut Zhen Peng verfasserin aut Xiongming Du verfasserin aut Xiongming Du verfasserin aut Xiongming Du verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 12(2022) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:12 year:2022 https://doi.org/10.3389/fpls.2021.822198 kostenfrei https://doaj.org/article/0623de1413a44319b947537ee055a0d3 kostenfrei https://www.frontiersin.org/articles/10.3389/fpls.2021.822198/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022
allfieldsGer	10.3389/fpls.2021.822198 doi (DE-627)DOAJ045490481 (DE-599)DOAJ0623de1413a44319b947537ee055a0d3 DE-627 ger DE-627 rakwb eng SB1-1110 Zhenzhen Wang verfasserin aut Transcriptome Co-expression Network and Metabolome Analysis Identifies Key Genes and Regulators of Proanthocyanidins Biosynthesis in Brown Cotton 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Brown cotton fiber (BCF) is a unique raw material of naturally colored cotton (NCC). But characteristics of the regulatory gene network and metabolic components related to the proanthocyanidins biosynthesis pathway at various stages of its fiber development remain unclear. Here, the dynamic changes in proanthocyanidins biosynthesis components and transcripts in the BCF variety “Zong 1-61” and its white near-isogenic lines (NILs) “RT” were characterized at five fiber developmental stages (0, 5, 10, 15, and 20 days post-anthesis; DPA). Enrichment analysis of differentially expressed genes (DEGs), comparison of metabolome differences, and pathway enrichment analysis of a weighted gene correlation network analysis together revealed the dominant gene expression of flavonoid biosynthesis (FB), phenylpropanoid metabolisms, and some carbohydrate metabolisms at 15 or 20 DPA than white cotton. Eventually, 63 genes were identified from five modules putatively related to FB. Three R2R3-MYB and two bHLH transcription factors were predicted as the core genes. Further, GhANS, GhANR1, and GhUFGT2 were preliminarily regulated by GhMYB46, GhMYB6, and GhMYB3, respectively, according to yeast one-hybrid assays in vitro. Our findings provide an important transcriptional regulatory network of proanthocyanidins biosynthesis pathway and dynamic flavonoid metabolism profiles. brown cotton transcriptome metabolome flavonoid metabolism yeast one-hybrid R2R3-MYB genes Plant culture Xiaomeng Zhang verfasserin aut Shoupu He verfasserin aut Shoupu He verfasserin aut Shoupu He verfasserin aut Abdul Rehman verfasserin aut Yinhua Jia verfasserin aut Hongge Li verfasserin aut Hongge Li verfasserin aut Zhaoe Pan verfasserin aut Xiaoli Geng verfasserin aut Qiong Gao verfasserin aut Liru Wang verfasserin aut Zhen Peng verfasserin aut Zhen Peng verfasserin aut Zhen Peng verfasserin aut Xiongming Du verfasserin aut Xiongming Du verfasserin aut Xiongming Du verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 12(2022) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:12 year:2022 https://doi.org/10.3389/fpls.2021.822198 kostenfrei https://doaj.org/article/0623de1413a44319b947537ee055a0d3 kostenfrei https://www.frontiersin.org/articles/10.3389/fpls.2021.822198/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022
allfieldsSound	10.3389/fpls.2021.822198 doi (DE-627)DOAJ045490481 (DE-599)DOAJ0623de1413a44319b947537ee055a0d3 DE-627 ger DE-627 rakwb eng SB1-1110 Zhenzhen Wang verfasserin aut Transcriptome Co-expression Network and Metabolome Analysis Identifies Key Genes and Regulators of Proanthocyanidins Biosynthesis in Brown Cotton 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Brown cotton fiber (BCF) is a unique raw material of naturally colored cotton (NCC). But characteristics of the regulatory gene network and metabolic components related to the proanthocyanidins biosynthesis pathway at various stages of its fiber development remain unclear. Here, the dynamic changes in proanthocyanidins biosynthesis components and transcripts in the BCF variety “Zong 1-61” and its white near-isogenic lines (NILs) “RT” were characterized at five fiber developmental stages (0, 5, 10, 15, and 20 days post-anthesis; DPA). Enrichment analysis of differentially expressed genes (DEGs), comparison of metabolome differences, and pathway enrichment analysis of a weighted gene correlation network analysis together revealed the dominant gene expression of flavonoid biosynthesis (FB), phenylpropanoid metabolisms, and some carbohydrate metabolisms at 15 or 20 DPA than white cotton. Eventually, 63 genes were identified from five modules putatively related to FB. Three R2R3-MYB and two bHLH transcription factors were predicted as the core genes. Further, GhANS, GhANR1, and GhUFGT2 were preliminarily regulated by GhMYB46, GhMYB6, and GhMYB3, respectively, according to yeast one-hybrid assays in vitro. Our findings provide an important transcriptional regulatory network of proanthocyanidins biosynthesis pathway and dynamic flavonoid metabolism profiles. brown cotton transcriptome metabolome flavonoid metabolism yeast one-hybrid R2R3-MYB genes Plant culture Xiaomeng Zhang verfasserin aut Shoupu He verfasserin aut Shoupu He verfasserin aut Shoupu He verfasserin aut Abdul Rehman verfasserin aut Yinhua Jia verfasserin aut Hongge Li verfasserin aut Hongge Li verfasserin aut Zhaoe Pan verfasserin aut Xiaoli Geng verfasserin aut Qiong Gao verfasserin aut Liru Wang verfasserin aut Zhen Peng verfasserin aut Zhen Peng verfasserin aut Zhen Peng verfasserin aut Xiongming Du verfasserin aut Xiongming Du verfasserin aut Xiongming Du verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 12(2022) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:12 year:2022 https://doi.org/10.3389/fpls.2021.822198 kostenfrei https://doaj.org/article/0623de1413a44319b947537ee055a0d3 kostenfrei https://www.frontiersin.org/articles/10.3389/fpls.2021.822198/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 12 2022
language	English
source	In Frontiers in Plant Science 12(2022) volume:12 year:2022
sourceStr	In Frontiers in Plant Science 12(2022) volume:12 year:2022
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	brown cotton transcriptome metabolome flavonoid metabolism yeast one-hybrid R2R3-MYB genes Plant culture
isfreeaccess_bool	true
container_title	Frontiers in Plant Science
authorswithroles_txt_mv	Zhenzhen Wang @@aut@@ Xiaomeng Zhang @@aut@@ Shoupu He @@aut@@ Abdul Rehman @@aut@@ Yinhua Jia @@aut@@ Hongge Li @@aut@@ Zhaoe Pan @@aut@@ Xiaoli Geng @@aut@@ Qiong Gao @@aut@@ Liru Wang @@aut@@ Zhen Peng @@aut@@ Xiongming Du @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	662359240
id	DOAJ045490481
language_de	englisch
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callnumber-first	S - Agriculture
author	Zhenzhen Wang
spellingShingle	Zhenzhen Wang misc SB1-1110 misc brown cotton misc transcriptome misc metabolome misc flavonoid metabolism misc yeast one-hybrid misc R2R3-MYB genes misc Plant culture Transcriptome Co-expression Network and Metabolome Analysis Identifies Key Genes and Regulators of Proanthocyanidins Biosynthesis in Brown Cotton
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topic_title	SB1-1110 Transcriptome Co-expression Network and Metabolome Analysis Identifies Key Genes and Regulators of Proanthocyanidins Biosynthesis in Brown Cotton brown cotton transcriptome metabolome flavonoid metabolism yeast one-hybrid R2R3-MYB genes
topic	misc SB1-1110 misc brown cotton misc transcriptome misc metabolome misc flavonoid metabolism misc yeast one-hybrid misc R2R3-MYB genes misc Plant culture
topic_unstemmed	misc SB1-1110 misc brown cotton misc transcriptome misc metabolome misc flavonoid metabolism misc yeast one-hybrid misc R2R3-MYB genes misc Plant culture
topic_browse	misc SB1-1110 misc brown cotton misc transcriptome misc metabolome misc flavonoid metabolism misc yeast one-hybrid misc R2R3-MYB genes misc Plant culture
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title	Transcriptome Co-expression Network and Metabolome Analysis Identifies Key Genes and Regulators of Proanthocyanidins Biosynthesis in Brown Cotton
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title_full	Transcriptome Co-expression Network and Metabolome Analysis Identifies Key Genes and Regulators of Proanthocyanidins Biosynthesis in Brown Cotton
author_sort	Zhenzhen Wang
journal	Frontiers in Plant Science
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author_browse	Zhenzhen Wang Xiaomeng Zhang Shoupu He Abdul Rehman Yinhua Jia Hongge Li Zhaoe Pan Xiaoli Geng Qiong Gao Liru Wang Zhen Peng Xiongming Du
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title_sort	transcriptome co-expression network and metabolome analysis identifies key genes and regulators of proanthocyanidins biosynthesis in brown cotton
callnumber	SB1-1110
title_auth	Transcriptome Co-expression Network and Metabolome Analysis Identifies Key Genes and Regulators of Proanthocyanidins Biosynthesis in Brown Cotton
abstract	Brown cotton fiber (BCF) is a unique raw material of naturally colored cotton (NCC). But characteristics of the regulatory gene network and metabolic components related to the proanthocyanidins biosynthesis pathway at various stages of its fiber development remain unclear. Here, the dynamic changes in proanthocyanidins biosynthesis components and transcripts in the BCF variety “Zong 1-61” and its white near-isogenic lines (NILs) “RT” were characterized at five fiber developmental stages (0, 5, 10, 15, and 20 days post-anthesis; DPA). Enrichment analysis of differentially expressed genes (DEGs), comparison of metabolome differences, and pathway enrichment analysis of a weighted gene correlation network analysis together revealed the dominant gene expression of flavonoid biosynthesis (FB), phenylpropanoid metabolisms, and some carbohydrate metabolisms at 15 or 20 DPA than white cotton. Eventually, 63 genes were identified from five modules putatively related to FB. Three R2R3-MYB and two bHLH transcription factors were predicted as the core genes. Further, GhANS, GhANR1, and GhUFGT2 were preliminarily regulated by GhMYB46, GhMYB6, and GhMYB3, respectively, according to yeast one-hybrid assays in vitro. Our findings provide an important transcriptional regulatory network of proanthocyanidins biosynthesis pathway and dynamic flavonoid metabolism profiles.
abstractGer	Brown cotton fiber (BCF) is a unique raw material of naturally colored cotton (NCC). But characteristics of the regulatory gene network and metabolic components related to the proanthocyanidins biosynthesis pathway at various stages of its fiber development remain unclear. Here, the dynamic changes in proanthocyanidins biosynthesis components and transcripts in the BCF variety “Zong 1-61” and its white near-isogenic lines (NILs) “RT” were characterized at five fiber developmental stages (0, 5, 10, 15, and 20 days post-anthesis; DPA). Enrichment analysis of differentially expressed genes (DEGs), comparison of metabolome differences, and pathway enrichment analysis of a weighted gene correlation network analysis together revealed the dominant gene expression of flavonoid biosynthesis (FB), phenylpropanoid metabolisms, and some carbohydrate metabolisms at 15 or 20 DPA than white cotton. Eventually, 63 genes were identified from five modules putatively related to FB. Three R2R3-MYB and two bHLH transcription factors were predicted as the core genes. Further, GhANS, GhANR1, and GhUFGT2 were preliminarily regulated by GhMYB46, GhMYB6, and GhMYB3, respectively, according to yeast one-hybrid assays in vitro. Our findings provide an important transcriptional regulatory network of proanthocyanidins biosynthesis pathway and dynamic flavonoid metabolism profiles.
abstract_unstemmed	Brown cotton fiber (BCF) is a unique raw material of naturally colored cotton (NCC). But characteristics of the regulatory gene network and metabolic components related to the proanthocyanidins biosynthesis pathway at various stages of its fiber development remain unclear. Here, the dynamic changes in proanthocyanidins biosynthesis components and transcripts in the BCF variety “Zong 1-61” and its white near-isogenic lines (NILs) “RT” were characterized at five fiber developmental stages (0, 5, 10, 15, and 20 days post-anthesis; DPA). Enrichment analysis of differentially expressed genes (DEGs), comparison of metabolome differences, and pathway enrichment analysis of a weighted gene correlation network analysis together revealed the dominant gene expression of flavonoid biosynthesis (FB), phenylpropanoid metabolisms, and some carbohydrate metabolisms at 15 or 20 DPA than white cotton. Eventually, 63 genes were identified from five modules putatively related to FB. Three R2R3-MYB and two bHLH transcription factors were predicted as the core genes. Further, GhANS, GhANR1, and GhUFGT2 were preliminarily regulated by GhMYB46, GhMYB6, and GhMYB3, respectively, according to yeast one-hybrid assays in vitro. Our findings provide an important transcriptional regulatory network of proanthocyanidins biosynthesis pathway and dynamic flavonoid metabolism profiles.
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title_short	Transcriptome Co-expression Network and Metabolome Analysis Identifies Key Genes and Regulators of Proanthocyanidins Biosynthesis in Brown Cotton
url	https://doi.org/10.3389/fpls.2021.822198 https://doaj.org/article/0623de1413a44319b947537ee055a0d3 https://www.frontiersin.org/articles/10.3389/fpls.2021.822198/full https://doaj.org/toc/1664-462X
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author2	Xiaomeng Zhang Shoupu He Abdul Rehman Yinhua Jia Hongge Li Zhaoe Pan Xiaoli Geng Qiong Gao Liru Wang Zhen Peng Xiongming Du
author2Str	Xiaomeng Zhang Shoupu He Abdul Rehman Yinhua Jia Hongge Li Zhaoe Pan Xiaoli Geng Qiong Gao Liru Wang Zhen Peng Xiongming Du
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Transcriptome Co-expression Network and Metabolome Analysis Identifies Key Genes and Regulators of Proanthocyanidins Biosynthesis in Brown Cotton

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