Expression Profiling of Lipolytic and Long-Chain Fatty Acid Biosynthesis Genes in Catfish Larvae Fed With Graded Lipid Levels

This study was designed to evaluate the effect of dietary lipid levels on the mRNA transcripts of lipid metabolic regulatory genes. Nine combinations of experimental diets containing graded lipid (80, 100, and 120 g/kg) and protein (450, 500, and 550 g/kg) levels were fed to the Clarias magur (India...
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Gespeichert in:

Autor*in:	Ishfaq Nazir Mir [verfasserIn] P. P. Srivastava [verfasserIn] Irfan Ahmad Bhat [verfasserIn] Jaffer Yousuf Dar [verfasserIn] Deepak Agarwal [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	dietary lipid lipolytic desaturase elongase transcript Clarias magur

Übergeordnetes Werk:	In: Frontiers in Marine Science - Frontiers Media S.A., 2015, 7(2020)
Übergeordnetes Werk:	volume:7 ; year:2020

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fmars.2020.561402

Katalog-ID:	DOAJ045584966

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topic_facet	dietary lipid lipolytic desaturase elongase transcript Clarias magur Science Q General. Including nature conservation, geographical distribution
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container_title	Frontiers in Marine Science
authorswithroles_txt_mv	Ishfaq Nazir Mir @@aut@@ P. P. Srivastava @@aut@@ Irfan Ahmad Bhat @@aut@@ Jaffer Yousuf Dar @@aut@@ Deepak Agarwal @@aut@@
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callnumber-first	Q - Science
author	Ishfaq Nazir Mir
spellingShingle	Ishfaq Nazir Mir misc QH1-199.5 misc dietary lipid misc lipolytic misc desaturase misc elongase misc transcript misc Clarias magur misc Science misc Q misc General. Including nature conservation, geographical distribution Expression Profiling of Lipolytic and Long-Chain Fatty Acid Biosynthesis Genes in Catfish Larvae Fed With Graded Lipid Levels
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topic_title	QH1-199.5 Expression Profiling of Lipolytic and Long-Chain Fatty Acid Biosynthesis Genes in Catfish Larvae Fed With Graded Lipid Levels dietary lipid lipolytic desaturase elongase transcript Clarias magur
topic	misc QH1-199.5 misc dietary lipid misc lipolytic misc desaturase misc elongase misc transcript misc Clarias magur misc Science misc Q misc General. Including nature conservation, geographical distribution
topic_unstemmed	misc QH1-199.5 misc dietary lipid misc lipolytic misc desaturase misc elongase misc transcript misc Clarias magur misc Science misc Q misc General. Including nature conservation, geographical distribution
topic_browse	misc QH1-199.5 misc dietary lipid misc lipolytic misc desaturase misc elongase misc transcript misc Clarias magur misc Science misc Q misc General. Including nature conservation, geographical distribution
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title	Expression Profiling of Lipolytic and Long-Chain Fatty Acid Biosynthesis Genes in Catfish Larvae Fed With Graded Lipid Levels
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title_full	Expression Profiling of Lipolytic and Long-Chain Fatty Acid Biosynthesis Genes in Catfish Larvae Fed With Graded Lipid Levels
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author_browse	Ishfaq Nazir Mir P. P. Srivastava Irfan Ahmad Bhat Jaffer Yousuf Dar Deepak Agarwal
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title_sort	expression profiling of lipolytic and long-chain fatty acid biosynthesis genes in catfish larvae fed with graded lipid levels
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title_auth	Expression Profiling of Lipolytic and Long-Chain Fatty Acid Biosynthesis Genes in Catfish Larvae Fed With Graded Lipid Levels
abstract	This study was designed to evaluate the effect of dietary lipid levels on the mRNA transcripts of lipid metabolic regulatory genes. Nine combinations of experimental diets containing graded lipid (80, 100, and 120 g/kg) and protein (450, 500, and 550 g/kg) levels were fed to the Clarias magur (Indian walking catfish) larvae from 14 to 35 dph (day post hatching). All the lipolytic genes, such as pancreatic triacylglycerol lipase (PL), lipoprotein lipase (LPL), and bile salt-activated lipase (BAL), and genes for long-chain polyunsaturated fatty acid (LC-PUFA) biosynthetic enzymes like fatty acyl desaturase-2 (FADS2), fatty acyl desaturase-5 (FADS5), and elongase (ELOV) were expressed in a wide range of tissues. A high abundance of mRNA transcript levels of lipolytic genes was detected in the intestine and liver, and similarly, desaturases and elongase were predominantly found to be expressed in the liver, brain, and intestine. Among the diets, a significantly high expression of both lipolytic and LC-PUFA biosynthetic genes were observed at 8% dietary lipid level. The mRNA expression of all the studied genes was down-regulated at 12% dietary lipid contents. Hence, the present study concludes that the efficient nutrient utilization and the lipid metabolic pathway occur at the optimum dietary lipid level of 8% in C. magur larvae.
abstractGer	This study was designed to evaluate the effect of dietary lipid levels on the mRNA transcripts of lipid metabolic regulatory genes. Nine combinations of experimental diets containing graded lipid (80, 100, and 120 g/kg) and protein (450, 500, and 550 g/kg) levels were fed to the Clarias magur (Indian walking catfish) larvae from 14 to 35 dph (day post hatching). All the lipolytic genes, such as pancreatic triacylglycerol lipase (PL), lipoprotein lipase (LPL), and bile salt-activated lipase (BAL), and genes for long-chain polyunsaturated fatty acid (LC-PUFA) biosynthetic enzymes like fatty acyl desaturase-2 (FADS2), fatty acyl desaturase-5 (FADS5), and elongase (ELOV) were expressed in a wide range of tissues. A high abundance of mRNA transcript levels of lipolytic genes was detected in the intestine and liver, and similarly, desaturases and elongase were predominantly found to be expressed in the liver, brain, and intestine. Among the diets, a significantly high expression of both lipolytic and LC-PUFA biosynthetic genes were observed at 8% dietary lipid level. The mRNA expression of all the studied genes was down-regulated at 12% dietary lipid contents. Hence, the present study concludes that the efficient nutrient utilization and the lipid metabolic pathway occur at the optimum dietary lipid level of 8% in C. magur larvae.
abstract_unstemmed	This study was designed to evaluate the effect of dietary lipid levels on the mRNA transcripts of lipid metabolic regulatory genes. Nine combinations of experimental diets containing graded lipid (80, 100, and 120 g/kg) and protein (450, 500, and 550 g/kg) levels were fed to the Clarias magur (Indian walking catfish) larvae from 14 to 35 dph (day post hatching). All the lipolytic genes, such as pancreatic triacylglycerol lipase (PL), lipoprotein lipase (LPL), and bile salt-activated lipase (BAL), and genes for long-chain polyunsaturated fatty acid (LC-PUFA) biosynthetic enzymes like fatty acyl desaturase-2 (FADS2), fatty acyl desaturase-5 (FADS5), and elongase (ELOV) were expressed in a wide range of tissues. A high abundance of mRNA transcript levels of lipolytic genes was detected in the intestine and liver, and similarly, desaturases and elongase were predominantly found to be expressed in the liver, brain, and intestine. Among the diets, a significantly high expression of both lipolytic and LC-PUFA biosynthetic genes were observed at 8% dietary lipid level. The mRNA expression of all the studied genes was down-regulated at 12% dietary lipid contents. Hence, the present study concludes that the efficient nutrient utilization and the lipid metabolic pathway occur at the optimum dietary lipid level of 8% in C. magur larvae.
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title_short	Expression Profiling of Lipolytic and Long-Chain Fatty Acid Biosynthesis Genes in Catfish Larvae Fed With Graded Lipid Levels
url	https://doi.org/10.3389/fmars.2020.561402 https://doaj.org/article/465b27b392004d0fab248e92b9367ab2 https://www.frontiersin.org/article/10.3389/fmars.2020.561402/full https://doaj.org/toc/2296-7745
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