Effects of Manganese Porphyrins on Cellular Sulfur Metabolism

Manganese porphyrins (MnPs), MnTE-2-PyP<sup<5+</sup<, MnTnHex-2-PyP<sup<5+</sup< and MnTnBuOE-2-PyP<sup<5+</sup<, are superoxide dismutase (SOD) mimetics and form a redox cycle between O<sub<2</sub< and reductants, including ascorbic acid, ultimately p...
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Autor*in:	Kenneth R. Olson [verfasserIn] Yan Gao [verfasserIn] Andrea K. Steiger [verfasserIn] Michael D. Pluth [verfasserIn] Charles R. Tessier [verfasserIn] Troy A. Markel [verfasserIn] David Boone [verfasserIn] Robert V. Stahelin [verfasserIn] Ines Batinic-Haberle [verfasserIn] Karl D. Straubg [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	reactive sulfide species ros antioxidants mn porphyrins sod mimetics h polysulfides

Übergeordnetes Werk:	In: Molecules - MDPI AG, 2003, 25(2020), 4, p 980
Übergeordnetes Werk:	volume:25 ; year:2020 ; number:4, p 980

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/molecules25040980

Katalog-ID:	DOAJ046029036

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allfields	10.3390/molecules25040980 doi (DE-627)DOAJ046029036 (DE-599)DOAJ175883133928472f8a1e4bd315c8543f DE-627 ger DE-627 rakwb eng QD241-441 Kenneth R. Olson verfasserin aut Effects of Manganese Porphyrins on Cellular Sulfur Metabolism 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Manganese porphyrins (MnPs), MnTE-2-PyP<sup<5+</sup<, MnTnHex-2-PyP<sup<5+</sup< and MnTnBuOE-2-PyP<sup<5+</sup<, are superoxide dismutase (SOD) mimetics and form a redox cycle between O<sub<2</sub< and reductants, including ascorbic acid, ultimately producing hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<). We previously found that MnPs oxidize hydrogen sulfide (H<sub<2</sub<S) to polysulfides (PS; H<sub<2</sub<S<sub<n</sub<, n = 2−6) in buffer. Here, we examine the effects of MnPs for 24 h on H<sub<2</sub<S metabolism and PS production in HEK293, A549, HT29 and bone marrow derived stem cells (BMDSC) using H<sub<2</sub<S (AzMC, MeRho-AZ) and PS (SSP4) fluorophores. All MnPs decreased intracellular H<sub<2</sub<S production and increased intracellular PS. H<sub<2</sub<S metabolism and PS production were unaffected by cellular O<sub<2</sub< (5% versus 21% O<sub<2</sub<), H<sub<2</sub<O<sub<2</sub< or ascorbic acid. We observed with confocal microscopy that mitochondria are a major site of H<sub<2</sub<S production in HEK293 cells and that MnPs decrease mitochondrial H<sub<2</sub<S production and increase PS in what appeared to be nucleoli and cytosolic fibrillary elements. This supports a role for MnPs in the metabolism of H<sub<2</sub<S to PS, the latter serving as both short- and long-term antioxidants, and suggests that some of the biological effects of MnPs may be attributable to sulfur metabolism. reactive sulfide species ros antioxidants mn porphyrins sod mimetics h<sub<2</sub<s polysulfides Organic chemistry Yan Gao verfasserin aut Andrea K. Steiger verfasserin aut Michael D. Pluth verfasserin aut Charles R. Tessier verfasserin aut Troy A. Markel verfasserin aut David Boone verfasserin aut Robert V. Stahelin verfasserin aut Ines Batinic-Haberle verfasserin aut Karl D. Straubg verfasserin aut In Molecules MDPI AG, 2003 25(2020), 4, p 980 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:25 year:2020 number:4, p 980 https://doi.org/10.3390/molecules25040980 kostenfrei https://doaj.org/article/175883133928472f8a1e4bd315c8543f kostenfrei https://www.mdpi.com/1420-3049/25/4/980 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2020 4, p 980
spelling	10.3390/molecules25040980 doi (DE-627)DOAJ046029036 (DE-599)DOAJ175883133928472f8a1e4bd315c8543f DE-627 ger DE-627 rakwb eng QD241-441 Kenneth R. Olson verfasserin aut Effects of Manganese Porphyrins on Cellular Sulfur Metabolism 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Manganese porphyrins (MnPs), MnTE-2-PyP<sup<5+</sup<, MnTnHex-2-PyP<sup<5+</sup< and MnTnBuOE-2-PyP<sup<5+</sup<, are superoxide dismutase (SOD) mimetics and form a redox cycle between O<sub<2</sub< and reductants, including ascorbic acid, ultimately producing hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<). We previously found that MnPs oxidize hydrogen sulfide (H<sub<2</sub<S) to polysulfides (PS; H<sub<2</sub<S<sub<n</sub<, n = 2−6) in buffer. Here, we examine the effects of MnPs for 24 h on H<sub<2</sub<S metabolism and PS production in HEK293, A549, HT29 and bone marrow derived stem cells (BMDSC) using H<sub<2</sub<S (AzMC, MeRho-AZ) and PS (SSP4) fluorophores. All MnPs decreased intracellular H<sub<2</sub<S production and increased intracellular PS. H<sub<2</sub<S metabolism and PS production were unaffected by cellular O<sub<2</sub< (5% versus 21% O<sub<2</sub<), H<sub<2</sub<O<sub<2</sub< or ascorbic acid. We observed with confocal microscopy that mitochondria are a major site of H<sub<2</sub<S production in HEK293 cells and that MnPs decrease mitochondrial H<sub<2</sub<S production and increase PS in what appeared to be nucleoli and cytosolic fibrillary elements. This supports a role for MnPs in the metabolism of H<sub<2</sub<S to PS, the latter serving as both short- and long-term antioxidants, and suggests that some of the biological effects of MnPs may be attributable to sulfur metabolism. reactive sulfide species ros antioxidants mn porphyrins sod mimetics h<sub<2</sub<s polysulfides Organic chemistry Yan Gao verfasserin aut Andrea K. Steiger verfasserin aut Michael D. Pluth verfasserin aut Charles R. Tessier verfasserin aut Troy A. Markel verfasserin aut David Boone verfasserin aut Robert V. Stahelin verfasserin aut Ines Batinic-Haberle verfasserin aut Karl D. Straubg verfasserin aut In Molecules MDPI AG, 2003 25(2020), 4, p 980 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:25 year:2020 number:4, p 980 https://doi.org/10.3390/molecules25040980 kostenfrei https://doaj.org/article/175883133928472f8a1e4bd315c8543f kostenfrei https://www.mdpi.com/1420-3049/25/4/980 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2020 4, p 980
allfields_unstemmed	10.3390/molecules25040980 doi (DE-627)DOAJ046029036 (DE-599)DOAJ175883133928472f8a1e4bd315c8543f DE-627 ger DE-627 rakwb eng QD241-441 Kenneth R. Olson verfasserin aut Effects of Manganese Porphyrins on Cellular Sulfur Metabolism 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Manganese porphyrins (MnPs), MnTE-2-PyP<sup<5+</sup<, MnTnHex-2-PyP<sup<5+</sup< and MnTnBuOE-2-PyP<sup<5+</sup<, are superoxide dismutase (SOD) mimetics and form a redox cycle between O<sub<2</sub< and reductants, including ascorbic acid, ultimately producing hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<). We previously found that MnPs oxidize hydrogen sulfide (H<sub<2</sub<S) to polysulfides (PS; H<sub<2</sub<S<sub<n</sub<, n = 2−6) in buffer. Here, we examine the effects of MnPs for 24 h on H<sub<2</sub<S metabolism and PS production in HEK293, A549, HT29 and bone marrow derived stem cells (BMDSC) using H<sub<2</sub<S (AzMC, MeRho-AZ) and PS (SSP4) fluorophores. All MnPs decreased intracellular H<sub<2</sub<S production and increased intracellular PS. H<sub<2</sub<S metabolism and PS production were unaffected by cellular O<sub<2</sub< (5% versus 21% O<sub<2</sub<), H<sub<2</sub<O<sub<2</sub< or ascorbic acid. We observed with confocal microscopy that mitochondria are a major site of H<sub<2</sub<S production in HEK293 cells and that MnPs decrease mitochondrial H<sub<2</sub<S production and increase PS in what appeared to be nucleoli and cytosolic fibrillary elements. This supports a role for MnPs in the metabolism of H<sub<2</sub<S to PS, the latter serving as both short- and long-term antioxidants, and suggests that some of the biological effects of MnPs may be attributable to sulfur metabolism. reactive sulfide species ros antioxidants mn porphyrins sod mimetics h<sub<2</sub<s polysulfides Organic chemistry Yan Gao verfasserin aut Andrea K. Steiger verfasserin aut Michael D. Pluth verfasserin aut Charles R. Tessier verfasserin aut Troy A. Markel verfasserin aut David Boone verfasserin aut Robert V. Stahelin verfasserin aut Ines Batinic-Haberle verfasserin aut Karl D. Straubg verfasserin aut In Molecules MDPI AG, 2003 25(2020), 4, p 980 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:25 year:2020 number:4, p 980 https://doi.org/10.3390/molecules25040980 kostenfrei https://doaj.org/article/175883133928472f8a1e4bd315c8543f kostenfrei https://www.mdpi.com/1420-3049/25/4/980 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2020 4, p 980
allfieldsGer	10.3390/molecules25040980 doi (DE-627)DOAJ046029036 (DE-599)DOAJ175883133928472f8a1e4bd315c8543f DE-627 ger DE-627 rakwb eng QD241-441 Kenneth R. Olson verfasserin aut Effects of Manganese Porphyrins on Cellular Sulfur Metabolism 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Manganese porphyrins (MnPs), MnTE-2-PyP<sup<5+</sup<, MnTnHex-2-PyP<sup<5+</sup< and MnTnBuOE-2-PyP<sup<5+</sup<, are superoxide dismutase (SOD) mimetics and form a redox cycle between O<sub<2</sub< and reductants, including ascorbic acid, ultimately producing hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<). We previously found that MnPs oxidize hydrogen sulfide (H<sub<2</sub<S) to polysulfides (PS; H<sub<2</sub<S<sub<n</sub<, n = 2−6) in buffer. Here, we examine the effects of MnPs for 24 h on H<sub<2</sub<S metabolism and PS production in HEK293, A549, HT29 and bone marrow derived stem cells (BMDSC) using H<sub<2</sub<S (AzMC, MeRho-AZ) and PS (SSP4) fluorophores. All MnPs decreased intracellular H<sub<2</sub<S production and increased intracellular PS. H<sub<2</sub<S metabolism and PS production were unaffected by cellular O<sub<2</sub< (5% versus 21% O<sub<2</sub<), H<sub<2</sub<O<sub<2</sub< or ascorbic acid. We observed with confocal microscopy that mitochondria are a major site of H<sub<2</sub<S production in HEK293 cells and that MnPs decrease mitochondrial H<sub<2</sub<S production and increase PS in what appeared to be nucleoli and cytosolic fibrillary elements. This supports a role for MnPs in the metabolism of H<sub<2</sub<S to PS, the latter serving as both short- and long-term antioxidants, and suggests that some of the biological effects of MnPs may be attributable to sulfur metabolism. reactive sulfide species ros antioxidants mn porphyrins sod mimetics h<sub<2</sub<s polysulfides Organic chemistry Yan Gao verfasserin aut Andrea K. Steiger verfasserin aut Michael D. Pluth verfasserin aut Charles R. Tessier verfasserin aut Troy A. Markel verfasserin aut David Boone verfasserin aut Robert V. Stahelin verfasserin aut Ines Batinic-Haberle verfasserin aut Karl D. Straubg verfasserin aut In Molecules MDPI AG, 2003 25(2020), 4, p 980 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:25 year:2020 number:4, p 980 https://doi.org/10.3390/molecules25040980 kostenfrei https://doaj.org/article/175883133928472f8a1e4bd315c8543f kostenfrei https://www.mdpi.com/1420-3049/25/4/980 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2020 4, p 980
allfieldsSound	10.3390/molecules25040980 doi (DE-627)DOAJ046029036 (DE-599)DOAJ175883133928472f8a1e4bd315c8543f DE-627 ger DE-627 rakwb eng QD241-441 Kenneth R. Olson verfasserin aut Effects of Manganese Porphyrins on Cellular Sulfur Metabolism 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Manganese porphyrins (MnPs), MnTE-2-PyP<sup<5+</sup<, MnTnHex-2-PyP<sup<5+</sup< and MnTnBuOE-2-PyP<sup<5+</sup<, are superoxide dismutase (SOD) mimetics and form a redox cycle between O<sub<2</sub< and reductants, including ascorbic acid, ultimately producing hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<). We previously found that MnPs oxidize hydrogen sulfide (H<sub<2</sub<S) to polysulfides (PS; H<sub<2</sub<S<sub<n</sub<, n = 2−6) in buffer. Here, we examine the effects of MnPs for 24 h on H<sub<2</sub<S metabolism and PS production in HEK293, A549, HT29 and bone marrow derived stem cells (BMDSC) using H<sub<2</sub<S (AzMC, MeRho-AZ) and PS (SSP4) fluorophores. All MnPs decreased intracellular H<sub<2</sub<S production and increased intracellular PS. H<sub<2</sub<S metabolism and PS production were unaffected by cellular O<sub<2</sub< (5% versus 21% O<sub<2</sub<), H<sub<2</sub<O<sub<2</sub< or ascorbic acid. We observed with confocal microscopy that mitochondria are a major site of H<sub<2</sub<S production in HEK293 cells and that MnPs decrease mitochondrial H<sub<2</sub<S production and increase PS in what appeared to be nucleoli and cytosolic fibrillary elements. This supports a role for MnPs in the metabolism of H<sub<2</sub<S to PS, the latter serving as both short- and long-term antioxidants, and suggests that some of the biological effects of MnPs may be attributable to sulfur metabolism. reactive sulfide species ros antioxidants mn porphyrins sod mimetics h<sub<2</sub<s polysulfides Organic chemistry Yan Gao verfasserin aut Andrea K. Steiger verfasserin aut Michael D. Pluth verfasserin aut Charles R. Tessier verfasserin aut Troy A. Markel verfasserin aut David Boone verfasserin aut Robert V. Stahelin verfasserin aut Ines Batinic-Haberle verfasserin aut Karl D. Straubg verfasserin aut In Molecules MDPI AG, 2003 25(2020), 4, p 980 (DE-627)311313132 (DE-600)2008644-1 14203049 nnns volume:25 year:2020 number:4, p 980 https://doi.org/10.3390/molecules25040980 kostenfrei https://doaj.org/article/175883133928472f8a1e4bd315c8543f kostenfrei https://www.mdpi.com/1420-3049/25/4/980 kostenfrei https://doaj.org/toc/1420-3049 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2020 4, p 980
language	English
source	In Molecules 25(2020), 4, p 980 volume:25 year:2020 number:4, p 980
sourceStr	In Molecules 25(2020), 4, p 980 volume:25 year:2020 number:4, p 980
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	reactive sulfide species ros antioxidants mn porphyrins sod mimetics h<sub<2</sub<s polysulfides Organic chemistry
isfreeaccess_bool	true
container_title	Molecules
authorswithroles_txt_mv	Kenneth R. Olson @@aut@@ Yan Gao @@aut@@ Andrea K. Steiger @@aut@@ Michael D. Pluth @@aut@@ Charles R. Tessier @@aut@@ Troy A. Markel @@aut@@ David Boone @@aut@@ Robert V. Stahelin @@aut@@ Ines Batinic-Haberle @@aut@@ Karl D. Straubg @@aut@@
publishDateDaySort_date	2020-01-01T00:00:00Z
hierarchy_top_id	311313132
id	DOAJ046029036
language_de	englisch
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callnumber-first	Q - Science
author	Kenneth R. Olson
spellingShingle	Kenneth R. Olson misc QD241-441 misc reactive sulfide species misc ros misc antioxidants misc mn porphyrins misc sod mimetics misc h<sub<2</sub<s misc polysulfides misc Organic chemistry Effects of Manganese Porphyrins on Cellular Sulfur Metabolism
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topic_title	QD241-441 Effects of Manganese Porphyrins on Cellular Sulfur Metabolism reactive sulfide species ros antioxidants mn porphyrins sod mimetics h<sub<2</sub<s polysulfides
topic	misc QD241-441 misc reactive sulfide species misc ros misc antioxidants misc mn porphyrins misc sod mimetics misc h<sub<2</sub<s misc polysulfides misc Organic chemistry
topic_unstemmed	misc QD241-441 misc reactive sulfide species misc ros misc antioxidants misc mn porphyrins misc sod mimetics misc h<sub<2</sub<s misc polysulfides misc Organic chemistry
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title	Effects of Manganese Porphyrins on Cellular Sulfur Metabolism
ctrlnum	(DE-627)DOAJ046029036 (DE-599)DOAJ175883133928472f8a1e4bd315c8543f
title_full	Effects of Manganese Porphyrins on Cellular Sulfur Metabolism
author_sort	Kenneth R. Olson
journal	Molecules
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author_browse	Kenneth R. Olson Yan Gao Andrea K. Steiger Michael D. Pluth Charles R. Tessier Troy A. Markel David Boone Robert V. Stahelin Ines Batinic-Haberle Karl D. Straubg
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author-letter	Kenneth R. Olson
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title_sort	effects of manganese porphyrins on cellular sulfur metabolism
callnumber	QD241-441
title_auth	Effects of Manganese Porphyrins on Cellular Sulfur Metabolism
abstract	Manganese porphyrins (MnPs), MnTE-2-PyP<sup<5+</sup<, MnTnHex-2-PyP<sup<5+</sup< and MnTnBuOE-2-PyP<sup<5+</sup<, are superoxide dismutase (SOD) mimetics and form a redox cycle between O<sub<2</sub< and reductants, including ascorbic acid, ultimately producing hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<). We previously found that MnPs oxidize hydrogen sulfide (H<sub<2</sub<S) to polysulfides (PS; H<sub<2</sub<S<sub<n</sub<, n = 2−6) in buffer. Here, we examine the effects of MnPs for 24 h on H<sub<2</sub<S metabolism and PS production in HEK293, A549, HT29 and bone marrow derived stem cells (BMDSC) using H<sub<2</sub<S (AzMC, MeRho-AZ) and PS (SSP4) fluorophores. All MnPs decreased intracellular H<sub<2</sub<S production and increased intracellular PS. H<sub<2</sub<S metabolism and PS production were unaffected by cellular O<sub<2</sub< (5% versus 21% O<sub<2</sub<), H<sub<2</sub<O<sub<2</sub< or ascorbic acid. We observed with confocal microscopy that mitochondria are a major site of H<sub<2</sub<S production in HEK293 cells and that MnPs decrease mitochondrial H<sub<2</sub<S production and increase PS in what appeared to be nucleoli and cytosolic fibrillary elements. This supports a role for MnPs in the metabolism of H<sub<2</sub<S to PS, the latter serving as both short- and long-term antioxidants, and suggests that some of the biological effects of MnPs may be attributable to sulfur metabolism.
abstractGer	Manganese porphyrins (MnPs), MnTE-2-PyP<sup<5+</sup<, MnTnHex-2-PyP<sup<5+</sup< and MnTnBuOE-2-PyP<sup<5+</sup<, are superoxide dismutase (SOD) mimetics and form a redox cycle between O<sub<2</sub< and reductants, including ascorbic acid, ultimately producing hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<). We previously found that MnPs oxidize hydrogen sulfide (H<sub<2</sub<S) to polysulfides (PS; H<sub<2</sub<S<sub<n</sub<, n = 2−6) in buffer. Here, we examine the effects of MnPs for 24 h on H<sub<2</sub<S metabolism and PS production in HEK293, A549, HT29 and bone marrow derived stem cells (BMDSC) using H<sub<2</sub<S (AzMC, MeRho-AZ) and PS (SSP4) fluorophores. All MnPs decreased intracellular H<sub<2</sub<S production and increased intracellular PS. H<sub<2</sub<S metabolism and PS production were unaffected by cellular O<sub<2</sub< (5% versus 21% O<sub<2</sub<), H<sub<2</sub<O<sub<2</sub< or ascorbic acid. We observed with confocal microscopy that mitochondria are a major site of H<sub<2</sub<S production in HEK293 cells and that MnPs decrease mitochondrial H<sub<2</sub<S production and increase PS in what appeared to be nucleoli and cytosolic fibrillary elements. This supports a role for MnPs in the metabolism of H<sub<2</sub<S to PS, the latter serving as both short- and long-term antioxidants, and suggests that some of the biological effects of MnPs may be attributable to sulfur metabolism.
abstract_unstemmed	Manganese porphyrins (MnPs), MnTE-2-PyP<sup<5+</sup<, MnTnHex-2-PyP<sup<5+</sup< and MnTnBuOE-2-PyP<sup<5+</sup<, are superoxide dismutase (SOD) mimetics and form a redox cycle between O<sub<2</sub< and reductants, including ascorbic acid, ultimately producing hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<). We previously found that MnPs oxidize hydrogen sulfide (H<sub<2</sub<S) to polysulfides (PS; H<sub<2</sub<S<sub<n</sub<, n = 2−6) in buffer. Here, we examine the effects of MnPs for 24 h on H<sub<2</sub<S metabolism and PS production in HEK293, A549, HT29 and bone marrow derived stem cells (BMDSC) using H<sub<2</sub<S (AzMC, MeRho-AZ) and PS (SSP4) fluorophores. All MnPs decreased intracellular H<sub<2</sub<S production and increased intracellular PS. H<sub<2</sub<S metabolism and PS production were unaffected by cellular O<sub<2</sub< (5% versus 21% O<sub<2</sub<), H<sub<2</sub<O<sub<2</sub< or ascorbic acid. We observed with confocal microscopy that mitochondria are a major site of H<sub<2</sub<S production in HEK293 cells and that MnPs decrease mitochondrial H<sub<2</sub<S production and increase PS in what appeared to be nucleoli and cytosolic fibrillary elements. This supports a role for MnPs in the metabolism of H<sub<2</sub<S to PS, the latter serving as both short- and long-term antioxidants, and suggests that some of the biological effects of MnPs may be attributable to sulfur metabolism.
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title_short	Effects of Manganese Porphyrins on Cellular Sulfur Metabolism
url	https://doi.org/10.3390/molecules25040980 https://doaj.org/article/175883133928472f8a1e4bd315c8543f https://www.mdpi.com/1420-3049/25/4/980 https://doaj.org/toc/1420-3049
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up_date	2024-07-03T18:23:19.004Z
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