Dermal mesenchymal stem cells: a resource of migration-associated function in psoriasis?

Abstract Background Psoriasis is a chronic and systemic, immune-mediated, inflammatory disease. Mesenchymal stem cells have effects on the inflammatory microenvironment, including regulating the proliferation, differentiation, recruitment, and migration of immunocytes. Methods To investigate whether...
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Autor*in:	Xuping Niu [verfasserIn] Junqing Li [verfasserIn] Xincheng Zhao [verfasserIn] Qiang Wang [verfasserIn] Gang Wang [verfasserIn] Ruixia Hou [verfasserIn] Xinhua Li [verfasserIn] Peng An [verfasserIn] Guohua Yin [verfasserIn] Kaiming Zhang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	Psoriasis Mesenchymal stem cells Migration Quantitative real-time reverse transcription PCR Western blot

Übergeordnetes Werk:	In: Stem Cell Research & Therapy - BMC, 2015, 10(2019), 1, Seite 8
Übergeordnetes Werk:	volume:10 ; year:2019 ; number:1 ; pages:8

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/s13287-019-1159-3

Katalog-ID:	DOAJ046494332

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520			\|a Abstract Background Psoriasis is a chronic and systemic, immune-mediated, inflammatory disease. Mesenchymal stem cells have effects on the inflammatory microenvironment, including regulating the proliferation, differentiation, recruitment, and migration of immunocytes. Methods To investigate whether dermal mesenchymal stem cells (DMSCs) may act on migration of immunocytes in psoriasis patients, 22 patients with psoriasis and 22 matching healthy controls (age and sex in this study) were recruited. Seven migration-associated genes including chemokine like receptor-1 (CMKLR-1), collagen type VIII alpha1 (COL8A-1), neuropilin and tolloid-like 2 (NETO-2), nik-related kinase (NRK), secreted frizzled-related protein (SFRP), sulfate 6-O-endosulfatase 2 (SULF-2), and synaptotagmin-like protein 2 (SYTL-2) were analyzed by quantitative real-time reverse transcription PCR and western blot. Peripheral blood-derived mononuclear cells (PBMCs) migration to MSCs was measured using a Thanswell chamber system. Results We observed the upregulation of CMKLR-1, COL8A-1, NETO-2, NRK, SYTL-2, and SULF-2 in dermal mesenchymal stem cells derived from patients with psoriasis at both mRNA and protein level, however, a significant downregulation of SFRP-2 between two groups. By contrast, there were no significant between-group differences at the mRNA and protein expression level of NETO-2 and SULF-2. The migration assay showed that in vitro the normal PBMC migration to psoriatic DMSC group was a 6.3 ± 0.7-fold increase compared with the control group. Conclusions The results may suggest a potential pathogenetic involvement of DMSCs on migration of monocytes in psoriasis. Immune responses are regulated at the level of DMSCs, which probably represent the cells primarily involved in the “psoriatic march.”
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allfields	10.1186/s13287-019-1159-3 doi (DE-627)DOAJ046494332 (DE-599)DOAJab2d99bcde9044cab53d07a1c5c40c43 DE-627 ger DE-627 rakwb eng R5-920 QD415-436 Xuping Niu verfasserin aut Dermal mesenchymal stem cells: a resource of migration-associated function in psoriasis? 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Psoriasis is a chronic and systemic, immune-mediated, inflammatory disease. Mesenchymal stem cells have effects on the inflammatory microenvironment, including regulating the proliferation, differentiation, recruitment, and migration of immunocytes. Methods To investigate whether dermal mesenchymal stem cells (DMSCs) may act on migration of immunocytes in psoriasis patients, 22 patients with psoriasis and 22 matching healthy controls (age and sex in this study) were recruited. Seven migration-associated genes including chemokine like receptor-1 (CMKLR-1), collagen type VIII alpha1 (COL8A-1), neuropilin and tolloid-like 2 (NETO-2), nik-related kinase (NRK), secreted frizzled-related protein (SFRP), sulfate 6-O-endosulfatase 2 (SULF-2), and synaptotagmin-like protein 2 (SYTL-2) were analyzed by quantitative real-time reverse transcription PCR and western blot. Peripheral blood-derived mononuclear cells (PBMCs) migration to MSCs was measured using a Thanswell chamber system. Results We observed the upregulation of CMKLR-1, COL8A-1, NETO-2, NRK, SYTL-2, and SULF-2 in dermal mesenchymal stem cells derived from patients with psoriasis at both mRNA and protein level, however, a significant downregulation of SFRP-2 between two groups. By contrast, there were no significant between-group differences at the mRNA and protein expression level of NETO-2 and SULF-2. The migration assay showed that in vitro the normal PBMC migration to psoriatic DMSC group was a 6.3 ± 0.7-fold increase compared with the control group. Conclusions The results may suggest a potential pathogenetic involvement of DMSCs on migration of monocytes in psoriasis. Immune responses are regulated at the level of DMSCs, which probably represent the cells primarily involved in the “psoriatic march.” Psoriasis Mesenchymal stem cells Migration Quantitative real-time reverse transcription PCR Western blot Medicine (General) Biochemistry Junqing Li verfasserin aut Xincheng Zhao verfasserin aut Qiang Wang verfasserin aut Gang Wang verfasserin aut Ruixia Hou verfasserin aut Xinhua Li verfasserin aut Peng An verfasserin aut Guohua Yin verfasserin aut Kaiming Zhang verfasserin aut In Stem Cell Research & Therapy BMC, 2015 10(2019), 1, Seite 8 (DE-627)624251047 (DE-600)2548671-8 17576512 nnns volume:10 year:2019 number:1 pages:8 https://doi.org/10.1186/s13287-019-1159-3 kostenfrei https://doaj.org/article/ab2d99bcde9044cab53d07a1c5c40c43 kostenfrei http://link.springer.com/article/10.1186/s13287-019-1159-3 kostenfrei https://doaj.org/toc/1757-6512 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2019 1 8
spelling	10.1186/s13287-019-1159-3 doi (DE-627)DOAJ046494332 (DE-599)DOAJab2d99bcde9044cab53d07a1c5c40c43 DE-627 ger DE-627 rakwb eng R5-920 QD415-436 Xuping Niu verfasserin aut Dermal mesenchymal stem cells: a resource of migration-associated function in psoriasis? 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Psoriasis is a chronic and systemic, immune-mediated, inflammatory disease. Mesenchymal stem cells have effects on the inflammatory microenvironment, including regulating the proliferation, differentiation, recruitment, and migration of immunocytes. Methods To investigate whether dermal mesenchymal stem cells (DMSCs) may act on migration of immunocytes in psoriasis patients, 22 patients with psoriasis and 22 matching healthy controls (age and sex in this study) were recruited. Seven migration-associated genes including chemokine like receptor-1 (CMKLR-1), collagen type VIII alpha1 (COL8A-1), neuropilin and tolloid-like 2 (NETO-2), nik-related kinase (NRK), secreted frizzled-related protein (SFRP), sulfate 6-O-endosulfatase 2 (SULF-2), and synaptotagmin-like protein 2 (SYTL-2) were analyzed by quantitative real-time reverse transcription PCR and western blot. Peripheral blood-derived mononuclear cells (PBMCs) migration to MSCs was measured using a Thanswell chamber system. Results We observed the upregulation of CMKLR-1, COL8A-1, NETO-2, NRK, SYTL-2, and SULF-2 in dermal mesenchymal stem cells derived from patients with psoriasis at both mRNA and protein level, however, a significant downregulation of SFRP-2 between two groups. By contrast, there were no significant between-group differences at the mRNA and protein expression level of NETO-2 and SULF-2. The migration assay showed that in vitro the normal PBMC migration to psoriatic DMSC group was a 6.3 ± 0.7-fold increase compared with the control group. Conclusions The results may suggest a potential pathogenetic involvement of DMSCs on migration of monocytes in psoriasis. Immune responses are regulated at the level of DMSCs, which probably represent the cells primarily involved in the “psoriatic march.” Psoriasis Mesenchymal stem cells Migration Quantitative real-time reverse transcription PCR Western blot Medicine (General) Biochemistry Junqing Li verfasserin aut Xincheng Zhao verfasserin aut Qiang Wang verfasserin aut Gang Wang verfasserin aut Ruixia Hou verfasserin aut Xinhua Li verfasserin aut Peng An verfasserin aut Guohua Yin verfasserin aut Kaiming Zhang verfasserin aut In Stem Cell Research & Therapy BMC, 2015 10(2019), 1, Seite 8 (DE-627)624251047 (DE-600)2548671-8 17576512 nnns volume:10 year:2019 number:1 pages:8 https://doi.org/10.1186/s13287-019-1159-3 kostenfrei https://doaj.org/article/ab2d99bcde9044cab53d07a1c5c40c43 kostenfrei http://link.springer.com/article/10.1186/s13287-019-1159-3 kostenfrei https://doaj.org/toc/1757-6512 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2019 1 8
allfields_unstemmed	10.1186/s13287-019-1159-3 doi (DE-627)DOAJ046494332 (DE-599)DOAJab2d99bcde9044cab53d07a1c5c40c43 DE-627 ger DE-627 rakwb eng R5-920 QD415-436 Xuping Niu verfasserin aut Dermal mesenchymal stem cells: a resource of migration-associated function in psoriasis? 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Psoriasis is a chronic and systemic, immune-mediated, inflammatory disease. Mesenchymal stem cells have effects on the inflammatory microenvironment, including regulating the proliferation, differentiation, recruitment, and migration of immunocytes. Methods To investigate whether dermal mesenchymal stem cells (DMSCs) may act on migration of immunocytes in psoriasis patients, 22 patients with psoriasis and 22 matching healthy controls (age and sex in this study) were recruited. Seven migration-associated genes including chemokine like receptor-1 (CMKLR-1), collagen type VIII alpha1 (COL8A-1), neuropilin and tolloid-like 2 (NETO-2), nik-related kinase (NRK), secreted frizzled-related protein (SFRP), sulfate 6-O-endosulfatase 2 (SULF-2), and synaptotagmin-like protein 2 (SYTL-2) were analyzed by quantitative real-time reverse transcription PCR and western blot. Peripheral blood-derived mononuclear cells (PBMCs) migration to MSCs was measured using a Thanswell chamber system. Results We observed the upregulation of CMKLR-1, COL8A-1, NETO-2, NRK, SYTL-2, and SULF-2 in dermal mesenchymal stem cells derived from patients with psoriasis at both mRNA and protein level, however, a significant downregulation of SFRP-2 between two groups. By contrast, there were no significant between-group differences at the mRNA and protein expression level of NETO-2 and SULF-2. The migration assay showed that in vitro the normal PBMC migration to psoriatic DMSC group was a 6.3 ± 0.7-fold increase compared with the control group. Conclusions The results may suggest a potential pathogenetic involvement of DMSCs on migration of monocytes in psoriasis. Immune responses are regulated at the level of DMSCs, which probably represent the cells primarily involved in the “psoriatic march.” Psoriasis Mesenchymal stem cells Migration Quantitative real-time reverse transcription PCR Western blot Medicine (General) Biochemistry Junqing Li verfasserin aut Xincheng Zhao verfasserin aut Qiang Wang verfasserin aut Gang Wang verfasserin aut Ruixia Hou verfasserin aut Xinhua Li verfasserin aut Peng An verfasserin aut Guohua Yin verfasserin aut Kaiming Zhang verfasserin aut In Stem Cell Research & Therapy BMC, 2015 10(2019), 1, Seite 8 (DE-627)624251047 (DE-600)2548671-8 17576512 nnns volume:10 year:2019 number:1 pages:8 https://doi.org/10.1186/s13287-019-1159-3 kostenfrei https://doaj.org/article/ab2d99bcde9044cab53d07a1c5c40c43 kostenfrei http://link.springer.com/article/10.1186/s13287-019-1159-3 kostenfrei https://doaj.org/toc/1757-6512 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2019 1 8
allfieldsGer	10.1186/s13287-019-1159-3 doi (DE-627)DOAJ046494332 (DE-599)DOAJab2d99bcde9044cab53d07a1c5c40c43 DE-627 ger DE-627 rakwb eng R5-920 QD415-436 Xuping Niu verfasserin aut Dermal mesenchymal stem cells: a resource of migration-associated function in psoriasis? 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Psoriasis is a chronic and systemic, immune-mediated, inflammatory disease. Mesenchymal stem cells have effects on the inflammatory microenvironment, including regulating the proliferation, differentiation, recruitment, and migration of immunocytes. Methods To investigate whether dermal mesenchymal stem cells (DMSCs) may act on migration of immunocytes in psoriasis patients, 22 patients with psoriasis and 22 matching healthy controls (age and sex in this study) were recruited. Seven migration-associated genes including chemokine like receptor-1 (CMKLR-1), collagen type VIII alpha1 (COL8A-1), neuropilin and tolloid-like 2 (NETO-2), nik-related kinase (NRK), secreted frizzled-related protein (SFRP), sulfate 6-O-endosulfatase 2 (SULF-2), and synaptotagmin-like protein 2 (SYTL-2) were analyzed by quantitative real-time reverse transcription PCR and western blot. Peripheral blood-derived mononuclear cells (PBMCs) migration to MSCs was measured using a Thanswell chamber system. Results We observed the upregulation of CMKLR-1, COL8A-1, NETO-2, NRK, SYTL-2, and SULF-2 in dermal mesenchymal stem cells derived from patients with psoriasis at both mRNA and protein level, however, a significant downregulation of SFRP-2 between two groups. By contrast, there were no significant between-group differences at the mRNA and protein expression level of NETO-2 and SULF-2. The migration assay showed that in vitro the normal PBMC migration to psoriatic DMSC group was a 6.3 ± 0.7-fold increase compared with the control group. Conclusions The results may suggest a potential pathogenetic involvement of DMSCs on migration of monocytes in psoriasis. Immune responses are regulated at the level of DMSCs, which probably represent the cells primarily involved in the “psoriatic march.” Psoriasis Mesenchymal stem cells Migration Quantitative real-time reverse transcription PCR Western blot Medicine (General) Biochemistry Junqing Li verfasserin aut Xincheng Zhao verfasserin aut Qiang Wang verfasserin aut Gang Wang verfasserin aut Ruixia Hou verfasserin aut Xinhua Li verfasserin aut Peng An verfasserin aut Guohua Yin verfasserin aut Kaiming Zhang verfasserin aut In Stem Cell Research & Therapy BMC, 2015 10(2019), 1, Seite 8 (DE-627)624251047 (DE-600)2548671-8 17576512 nnns volume:10 year:2019 number:1 pages:8 https://doi.org/10.1186/s13287-019-1159-3 kostenfrei https://doaj.org/article/ab2d99bcde9044cab53d07a1c5c40c43 kostenfrei http://link.springer.com/article/10.1186/s13287-019-1159-3 kostenfrei https://doaj.org/toc/1757-6512 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2019 1 8
allfieldsSound	10.1186/s13287-019-1159-3 doi (DE-627)DOAJ046494332 (DE-599)DOAJab2d99bcde9044cab53d07a1c5c40c43 DE-627 ger DE-627 rakwb eng R5-920 QD415-436 Xuping Niu verfasserin aut Dermal mesenchymal stem cells: a resource of migration-associated function in psoriasis? 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abstract Background Psoriasis is a chronic and systemic, immune-mediated, inflammatory disease. Mesenchymal stem cells have effects on the inflammatory microenvironment, including regulating the proliferation, differentiation, recruitment, and migration of immunocytes. Methods To investigate whether dermal mesenchymal stem cells (DMSCs) may act on migration of immunocytes in psoriasis patients, 22 patients with psoriasis and 22 matching healthy controls (age and sex in this study) were recruited. Seven migration-associated genes including chemokine like receptor-1 (CMKLR-1), collagen type VIII alpha1 (COL8A-1), neuropilin and tolloid-like 2 (NETO-2), nik-related kinase (NRK), secreted frizzled-related protein (SFRP), sulfate 6-O-endosulfatase 2 (SULF-2), and synaptotagmin-like protein 2 (SYTL-2) were analyzed by quantitative real-time reverse transcription PCR and western blot. Peripheral blood-derived mononuclear cells (PBMCs) migration to MSCs was measured using a Thanswell chamber system. Results We observed the upregulation of CMKLR-1, COL8A-1, NETO-2, NRK, SYTL-2, and SULF-2 in dermal mesenchymal stem cells derived from patients with psoriasis at both mRNA and protein level, however, a significant downregulation of SFRP-2 between two groups. By contrast, there were no significant between-group differences at the mRNA and protein expression level of NETO-2 and SULF-2. The migration assay showed that in vitro the normal PBMC migration to psoriatic DMSC group was a 6.3 ± 0.7-fold increase compared with the control group. Conclusions The results may suggest a potential pathogenetic involvement of DMSCs on migration of monocytes in psoriasis. Immune responses are regulated at the level of DMSCs, which probably represent the cells primarily involved in the “psoriatic march.” Psoriasis Mesenchymal stem cells Migration Quantitative real-time reverse transcription PCR Western blot Medicine (General) Biochemistry Junqing Li verfasserin aut Xincheng Zhao verfasserin aut Qiang Wang verfasserin aut Gang Wang verfasserin aut Ruixia Hou verfasserin aut Xinhua Li verfasserin aut Peng An verfasserin aut Guohua Yin verfasserin aut Kaiming Zhang verfasserin aut In Stem Cell Research & Therapy BMC, 2015 10(2019), 1, Seite 8 (DE-627)624251047 (DE-600)2548671-8 17576512 nnns volume:10 year:2019 number:1 pages:8 https://doi.org/10.1186/s13287-019-1159-3 kostenfrei https://doaj.org/article/ab2d99bcde9044cab53d07a1c5c40c43 kostenfrei http://link.springer.com/article/10.1186/s13287-019-1159-3 kostenfrei https://doaj.org/toc/1757-6512 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2019 1 8
language	English
source	In Stem Cell Research & Therapy 10(2019), 1, Seite 8 volume:10 year:2019 number:1 pages:8
sourceStr	In Stem Cell Research & Therapy 10(2019), 1, Seite 8 volume:10 year:2019 number:1 pages:8
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Psoriasis Mesenchymal stem cells Migration Quantitative real-time reverse transcription PCR Western blot Medicine (General) Biochemistry
isfreeaccess_bool	true
container_title	Stem Cell Research & Therapy
authorswithroles_txt_mv	Xuping Niu @@aut@@ Junqing Li @@aut@@ Xincheng Zhao @@aut@@ Qiang Wang @@aut@@ Gang Wang @@aut@@ Ruixia Hou @@aut@@ Xinhua Li @@aut@@ Peng An @@aut@@ Guohua Yin @@aut@@ Kaiming Zhang @@aut@@
publishDateDaySort_date	2019-01-01T00:00:00Z
hierarchy_top_id	624251047
id	DOAJ046494332
language_de	englisch
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Mesenchymal stem cells have effects on the inflammatory microenvironment, including regulating the proliferation, differentiation, recruitment, and migration of immunocytes. Methods To investigate whether dermal mesenchymal stem cells (DMSCs) may act on migration of immunocytes in psoriasis patients, 22 patients with psoriasis and 22 matching healthy controls (age and sex in this study) were recruited. Seven migration-associated genes including chemokine like receptor-1 (CMKLR-1), collagen type VIII alpha1 (COL8A-1), neuropilin and tolloid-like 2 (NETO-2), nik-related kinase (NRK), secreted frizzled-related protein (SFRP), sulfate 6-O-endosulfatase 2 (SULF-2), and synaptotagmin-like protein 2 (SYTL-2) were analyzed by quantitative real-time reverse transcription PCR and western blot. Peripheral blood-derived mononuclear cells (PBMCs) migration to MSCs was measured using a Thanswell chamber system. Results We observed the upregulation of CMKLR-1, COL8A-1, NETO-2, NRK, SYTL-2, and SULF-2 in dermal mesenchymal stem cells derived from patients with psoriasis at both mRNA and protein level, however, a significant downregulation of SFRP-2 between two groups. By contrast, there were no significant between-group differences at the mRNA and protein expression level of NETO-2 and SULF-2. The migration assay showed that in vitro the normal PBMC migration to psoriatic DMSC group was a 6.3 ± 0.7-fold increase compared with the control group. Conclusions The results may suggest a potential pathogenetic involvement of DMSCs on migration of monocytes in psoriasis. 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callnumber-first	R - Medicine
author	Xuping Niu
spellingShingle	Xuping Niu misc R5-920 misc QD415-436 misc Psoriasis misc Mesenchymal stem cells misc Migration misc Quantitative real-time reverse transcription PCR misc Western blot misc Medicine (General) misc Biochemistry Dermal mesenchymal stem cells: a resource of migration-associated function in psoriasis?
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topic_title	R5-920 QD415-436 Dermal mesenchymal stem cells: a resource of migration-associated function in psoriasis? Psoriasis Mesenchymal stem cells Migration Quantitative real-time reverse transcription PCR Western blot
topic	misc R5-920 misc QD415-436 misc Psoriasis misc Mesenchymal stem cells misc Migration misc Quantitative real-time reverse transcription PCR misc Western blot misc Medicine (General) misc Biochemistry
topic_unstemmed	misc R5-920 misc QD415-436 misc Psoriasis misc Mesenchymal stem cells misc Migration misc Quantitative real-time reverse transcription PCR misc Western blot misc Medicine (General) misc Biochemistry
topic_browse	misc R5-920 misc QD415-436 misc Psoriasis misc Mesenchymal stem cells misc Migration misc Quantitative real-time reverse transcription PCR misc Western blot misc Medicine (General) misc Biochemistry
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title	Dermal mesenchymal stem cells: a resource of migration-associated function in psoriasis?
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title_full	Dermal mesenchymal stem cells: a resource of migration-associated function in psoriasis?
author_sort	Xuping Niu
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author_browse	Xuping Niu Junqing Li Xincheng Zhao Qiang Wang Gang Wang Ruixia Hou Xinhua Li Peng An Guohua Yin Kaiming Zhang
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title_sort	dermal mesenchymal stem cells: a resource of migration-associated function in psoriasis?
callnumber	R5-920
title_auth	Dermal mesenchymal stem cells: a resource of migration-associated function in psoriasis?
abstract	Abstract Background Psoriasis is a chronic and systemic, immune-mediated, inflammatory disease. Mesenchymal stem cells have effects on the inflammatory microenvironment, including regulating the proliferation, differentiation, recruitment, and migration of immunocytes. Methods To investigate whether dermal mesenchymal stem cells (DMSCs) may act on migration of immunocytes in psoriasis patients, 22 patients with psoriasis and 22 matching healthy controls (age and sex in this study) were recruited. Seven migration-associated genes including chemokine like receptor-1 (CMKLR-1), collagen type VIII alpha1 (COL8A-1), neuropilin and tolloid-like 2 (NETO-2), nik-related kinase (NRK), secreted frizzled-related protein (SFRP), sulfate 6-O-endosulfatase 2 (SULF-2), and synaptotagmin-like protein 2 (SYTL-2) were analyzed by quantitative real-time reverse transcription PCR and western blot. Peripheral blood-derived mononuclear cells (PBMCs) migration to MSCs was measured using a Thanswell chamber system. Results We observed the upregulation of CMKLR-1, COL8A-1, NETO-2, NRK, SYTL-2, and SULF-2 in dermal mesenchymal stem cells derived from patients with psoriasis at both mRNA and protein level, however, a significant downregulation of SFRP-2 between two groups. By contrast, there were no significant between-group differences at the mRNA and protein expression level of NETO-2 and SULF-2. The migration assay showed that in vitro the normal PBMC migration to psoriatic DMSC group was a 6.3 ± 0.7-fold increase compared with the control group. Conclusions The results may suggest a potential pathogenetic involvement of DMSCs on migration of monocytes in psoriasis. Immune responses are regulated at the level of DMSCs, which probably represent the cells primarily involved in the “psoriatic march.”
abstractGer	Abstract Background Psoriasis is a chronic and systemic, immune-mediated, inflammatory disease. Mesenchymal stem cells have effects on the inflammatory microenvironment, including regulating the proliferation, differentiation, recruitment, and migration of immunocytes. Methods To investigate whether dermal mesenchymal stem cells (DMSCs) may act on migration of immunocytes in psoriasis patients, 22 patients with psoriasis and 22 matching healthy controls (age and sex in this study) were recruited. Seven migration-associated genes including chemokine like receptor-1 (CMKLR-1), collagen type VIII alpha1 (COL8A-1), neuropilin and tolloid-like 2 (NETO-2), nik-related kinase (NRK), secreted frizzled-related protein (SFRP), sulfate 6-O-endosulfatase 2 (SULF-2), and synaptotagmin-like protein 2 (SYTL-2) were analyzed by quantitative real-time reverse transcription PCR and western blot. Peripheral blood-derived mononuclear cells (PBMCs) migration to MSCs was measured using a Thanswell chamber system. Results We observed the upregulation of CMKLR-1, COL8A-1, NETO-2, NRK, SYTL-2, and SULF-2 in dermal mesenchymal stem cells derived from patients with psoriasis at both mRNA and protein level, however, a significant downregulation of SFRP-2 between two groups. By contrast, there were no significant between-group differences at the mRNA and protein expression level of NETO-2 and SULF-2. The migration assay showed that in vitro the normal PBMC migration to psoriatic DMSC group was a 6.3 ± 0.7-fold increase compared with the control group. Conclusions The results may suggest a potential pathogenetic involvement of DMSCs on migration of monocytes in psoriasis. Immune responses are regulated at the level of DMSCs, which probably represent the cells primarily involved in the “psoriatic march.”
abstract_unstemmed	Abstract Background Psoriasis is a chronic and systemic, immune-mediated, inflammatory disease. Mesenchymal stem cells have effects on the inflammatory microenvironment, including regulating the proliferation, differentiation, recruitment, and migration of immunocytes. Methods To investigate whether dermal mesenchymal stem cells (DMSCs) may act on migration of immunocytes in psoriasis patients, 22 patients with psoriasis and 22 matching healthy controls (age and sex in this study) were recruited. Seven migration-associated genes including chemokine like receptor-1 (CMKLR-1), collagen type VIII alpha1 (COL8A-1), neuropilin and tolloid-like 2 (NETO-2), nik-related kinase (NRK), secreted frizzled-related protein (SFRP), sulfate 6-O-endosulfatase 2 (SULF-2), and synaptotagmin-like protein 2 (SYTL-2) were analyzed by quantitative real-time reverse transcription PCR and western blot. Peripheral blood-derived mononuclear cells (PBMCs) migration to MSCs was measured using a Thanswell chamber system. Results We observed the upregulation of CMKLR-1, COL8A-1, NETO-2, NRK, SYTL-2, and SULF-2 in dermal mesenchymal stem cells derived from patients with psoriasis at both mRNA and protein level, however, a significant downregulation of SFRP-2 between two groups. By contrast, there were no significant between-group differences at the mRNA and protein expression level of NETO-2 and SULF-2. The migration assay showed that in vitro the normal PBMC migration to psoriatic DMSC group was a 6.3 ± 0.7-fold increase compared with the control group. Conclusions The results may suggest a potential pathogenetic involvement of DMSCs on migration of monocytes in psoriasis. Immune responses are regulated at the level of DMSCs, which probably represent the cells primarily involved in the “psoriatic march.”
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title_short	Dermal mesenchymal stem cells: a resource of migration-associated function in psoriasis?
url	https://doi.org/10.1186/s13287-019-1159-3 https://doaj.org/article/ab2d99bcde9044cab53d07a1c5c40c43 http://link.springer.com/article/10.1186/s13287-019-1159-3 https://doaj.org/toc/1757-6512
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up_date	2024-07-03T20:58:12.855Z
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