m6A-label-seq: A metabolic labeling protocol to detect transcriptome-wide mRNA N6-methyladenosine (m6A) at base resolution

Summary: We describe here a metabolic labeling protocol for detecting cellular transcriptome-wide mRNA N6-methyladenosine (m6A) at base resolution. By feeding cells an analog of methionine, potential mRNA m6A forming sites are replaced with the N6-allyladenosine (a6A). A mild chemical iodination of...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Xiao Shu [verfasserIn] Jie Cao [verfasserIn] Jianzhao Liu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	sequence analysis cell culture sequencing RNA-seq molecular biology molecular/chemical probes

Übergeordnetes Werk:	In: STAR Protocols - Elsevier, 2020, 3(2022), 1, Seite 101096-
Übergeordnetes Werk:	volume:3 ; year:2022 ; number:1 ; pages:101096-

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.xpro.2021.101096

Katalog-ID:	DOAJ046895779

Internformat


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520			\|a Summary: We describe here a metabolic labeling protocol for detecting cellular transcriptome-wide mRNA N6-methyladenosine (m6A) at base resolution. By feeding cells an analog of methionine, potential mRNA m6A forming sites are replaced with the N6-allyladenosine (a6A). A mild chemical iodination of a6A in RNA results in its opposite base misincorporation during RNA reverse transcription, and thus m6A locations could be precisely identified in the high-throughput sequencing. m6A-label-seq provides a strategy to label and identify cellular epitranscriptomic modification sites.For complete details on the use and execution of this profile, please refer to Shu et al., 2020.
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allfields	10.1016/j.xpro.2021.101096 doi (DE-627)DOAJ046895779 (DE-599)DOAJa705763d4b0a4f1ea8a2184d10fa601b DE-627 ger DE-627 rakwb eng Q1-390 Xiao Shu verfasserin aut m6A-label-seq: A metabolic labeling protocol to detect transcriptome-wide mRNA N6-methyladenosine (m6A) at base resolution 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: We describe here a metabolic labeling protocol for detecting cellular transcriptome-wide mRNA N6-methyladenosine (m6A) at base resolution. By feeding cells an analog of methionine, potential mRNA m6A forming sites are replaced with the N6-allyladenosine (a6A). A mild chemical iodination of a6A in RNA results in its opposite base misincorporation during RNA reverse transcription, and thus m6A locations could be precisely identified in the high-throughput sequencing. m6A-label-seq provides a strategy to label and identify cellular epitranscriptomic modification sites.For complete details on the use and execution of this profile, please refer to Shu et al., 2020. sequence analysis cell culture sequencing RNA-seq molecular biology molecular/chemical probes Science (General) Jie Cao verfasserin aut Jianzhao Liu verfasserin aut In STAR Protocols Elsevier, 2020 3(2022), 1, Seite 101096- (DE-627)1747970557 26661667 nnns volume:3 year:2022 number:1 pages:101096- https://doi.org/10.1016/j.xpro.2021.101096 kostenfrei https://doaj.org/article/a705763d4b0a4f1ea8a2184d10fa601b kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166721008029 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 3 2022 1 101096-
spelling	10.1016/j.xpro.2021.101096 doi (DE-627)DOAJ046895779 (DE-599)DOAJa705763d4b0a4f1ea8a2184d10fa601b DE-627 ger DE-627 rakwb eng Q1-390 Xiao Shu verfasserin aut m6A-label-seq: A metabolic labeling protocol to detect transcriptome-wide mRNA N6-methyladenosine (m6A) at base resolution 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: We describe here a metabolic labeling protocol for detecting cellular transcriptome-wide mRNA N6-methyladenosine (m6A) at base resolution. By feeding cells an analog of methionine, potential mRNA m6A forming sites are replaced with the N6-allyladenosine (a6A). A mild chemical iodination of a6A in RNA results in its opposite base misincorporation during RNA reverse transcription, and thus m6A locations could be precisely identified in the high-throughput sequencing. m6A-label-seq provides a strategy to label and identify cellular epitranscriptomic modification sites.For complete details on the use and execution of this profile, please refer to Shu et al., 2020. sequence analysis cell culture sequencing RNA-seq molecular biology molecular/chemical probes Science (General) Jie Cao verfasserin aut Jianzhao Liu verfasserin aut In STAR Protocols Elsevier, 2020 3(2022), 1, Seite 101096- (DE-627)1747970557 26661667 nnns volume:3 year:2022 number:1 pages:101096- https://doi.org/10.1016/j.xpro.2021.101096 kostenfrei https://doaj.org/article/a705763d4b0a4f1ea8a2184d10fa601b kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166721008029 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 3 2022 1 101096-
allfields_unstemmed	10.1016/j.xpro.2021.101096 doi (DE-627)DOAJ046895779 (DE-599)DOAJa705763d4b0a4f1ea8a2184d10fa601b DE-627 ger DE-627 rakwb eng Q1-390 Xiao Shu verfasserin aut m6A-label-seq: A metabolic labeling protocol to detect transcriptome-wide mRNA N6-methyladenosine (m6A) at base resolution 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: We describe here a metabolic labeling protocol for detecting cellular transcriptome-wide mRNA N6-methyladenosine (m6A) at base resolution. By feeding cells an analog of methionine, potential mRNA m6A forming sites are replaced with the N6-allyladenosine (a6A). A mild chemical iodination of a6A in RNA results in its opposite base misincorporation during RNA reverse transcription, and thus m6A locations could be precisely identified in the high-throughput sequencing. m6A-label-seq provides a strategy to label and identify cellular epitranscriptomic modification sites.For complete details on the use and execution of this profile, please refer to Shu et al., 2020. sequence analysis cell culture sequencing RNA-seq molecular biology molecular/chemical probes Science (General) Jie Cao verfasserin aut Jianzhao Liu verfasserin aut In STAR Protocols Elsevier, 2020 3(2022), 1, Seite 101096- (DE-627)1747970557 26661667 nnns volume:3 year:2022 number:1 pages:101096- https://doi.org/10.1016/j.xpro.2021.101096 kostenfrei https://doaj.org/article/a705763d4b0a4f1ea8a2184d10fa601b kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166721008029 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 3 2022 1 101096-
allfieldsGer	10.1016/j.xpro.2021.101096 doi (DE-627)DOAJ046895779 (DE-599)DOAJa705763d4b0a4f1ea8a2184d10fa601b DE-627 ger DE-627 rakwb eng Q1-390 Xiao Shu verfasserin aut m6A-label-seq: A metabolic labeling protocol to detect transcriptome-wide mRNA N6-methyladenosine (m6A) at base resolution 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: We describe here a metabolic labeling protocol for detecting cellular transcriptome-wide mRNA N6-methyladenosine (m6A) at base resolution. By feeding cells an analog of methionine, potential mRNA m6A forming sites are replaced with the N6-allyladenosine (a6A). A mild chemical iodination of a6A in RNA results in its opposite base misincorporation during RNA reverse transcription, and thus m6A locations could be precisely identified in the high-throughput sequencing. m6A-label-seq provides a strategy to label and identify cellular epitranscriptomic modification sites.For complete details on the use and execution of this profile, please refer to Shu et al., 2020. sequence analysis cell culture sequencing RNA-seq molecular biology molecular/chemical probes Science (General) Jie Cao verfasserin aut Jianzhao Liu verfasserin aut In STAR Protocols Elsevier, 2020 3(2022), 1, Seite 101096- (DE-627)1747970557 26661667 nnns volume:3 year:2022 number:1 pages:101096- https://doi.org/10.1016/j.xpro.2021.101096 kostenfrei https://doaj.org/article/a705763d4b0a4f1ea8a2184d10fa601b kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166721008029 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 3 2022 1 101096-
allfieldsSound	10.1016/j.xpro.2021.101096 doi (DE-627)DOAJ046895779 (DE-599)DOAJa705763d4b0a4f1ea8a2184d10fa601b DE-627 ger DE-627 rakwb eng Q1-390 Xiao Shu verfasserin aut m6A-label-seq: A metabolic labeling protocol to detect transcriptome-wide mRNA N6-methyladenosine (m6A) at base resolution 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: We describe here a metabolic labeling protocol for detecting cellular transcriptome-wide mRNA N6-methyladenosine (m6A) at base resolution. By feeding cells an analog of methionine, potential mRNA m6A forming sites are replaced with the N6-allyladenosine (a6A). A mild chemical iodination of a6A in RNA results in its opposite base misincorporation during RNA reverse transcription, and thus m6A locations could be precisely identified in the high-throughput sequencing. m6A-label-seq provides a strategy to label and identify cellular epitranscriptomic modification sites.For complete details on the use and execution of this profile, please refer to Shu et al., 2020. sequence analysis cell culture sequencing RNA-seq molecular biology molecular/chemical probes Science (General) Jie Cao verfasserin aut Jianzhao Liu verfasserin aut In STAR Protocols Elsevier, 2020 3(2022), 1, Seite 101096- (DE-627)1747970557 26661667 nnns volume:3 year:2022 number:1 pages:101096- https://doi.org/10.1016/j.xpro.2021.101096 kostenfrei https://doaj.org/article/a705763d4b0a4f1ea8a2184d10fa601b kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166721008029 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 3 2022 1 101096-
language	English
source	In STAR Protocols 3(2022), 1, Seite 101096- volume:3 year:2022 number:1 pages:101096-
sourceStr	In STAR Protocols 3(2022), 1, Seite 101096- volume:3 year:2022 number:1 pages:101096-
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	sequence analysis cell culture sequencing RNA-seq molecular biology molecular/chemical probes Science (General)
isfreeaccess_bool	true
container_title	STAR Protocols
authorswithroles_txt_mv	Xiao Shu @@aut@@ Jie Cao @@aut@@ Jianzhao Liu @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	1747970557
id	DOAJ046895779
language_de	englisch
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author	Xiao Shu
spellingShingle	Xiao Shu misc Q1-390 misc sequence analysis misc cell culture misc sequencing misc RNA-seq misc molecular biology misc molecular/chemical probes misc Science (General) m6A-label-seq: A metabolic labeling protocol to detect transcriptome-wide mRNA N6-methyladenosine (m6A) at base resolution
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topic_title	Q1-390 m6A-label-seq: A metabolic labeling protocol to detect transcriptome-wide mRNA N6-methyladenosine (m6A) at base resolution sequence analysis cell culture sequencing RNA-seq molecular biology molecular/chemical probes
topic	misc Q1-390 misc sequence analysis misc cell culture misc sequencing misc RNA-seq misc molecular biology misc molecular/chemical probes misc Science (General)
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title	m6A-label-seq: A metabolic labeling protocol to detect transcriptome-wide mRNA N6-methyladenosine (m6A) at base resolution
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title_full	m6A-label-seq: A metabolic labeling protocol to detect transcriptome-wide mRNA N6-methyladenosine (m6A) at base resolution
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title_sort	m6a-label-seq: a metabolic labeling protocol to detect transcriptome-wide mrna n6-methyladenosine (m6a) at base resolution
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title_auth	m6A-label-seq: A metabolic labeling protocol to detect transcriptome-wide mRNA N6-methyladenosine (m6A) at base resolution
abstract	Summary: We describe here a metabolic labeling protocol for detecting cellular transcriptome-wide mRNA N6-methyladenosine (m6A) at base resolution. By feeding cells an analog of methionine, potential mRNA m6A forming sites are replaced with the N6-allyladenosine (a6A). A mild chemical iodination of a6A in RNA results in its opposite base misincorporation during RNA reverse transcription, and thus m6A locations could be precisely identified in the high-throughput sequencing. m6A-label-seq provides a strategy to label and identify cellular epitranscriptomic modification sites.For complete details on the use and execution of this profile, please refer to Shu et al., 2020.
abstractGer	Summary: We describe here a metabolic labeling protocol for detecting cellular transcriptome-wide mRNA N6-methyladenosine (m6A) at base resolution. By feeding cells an analog of methionine, potential mRNA m6A forming sites are replaced with the N6-allyladenosine (a6A). A mild chemical iodination of a6A in RNA results in its opposite base misincorporation during RNA reverse transcription, and thus m6A locations could be precisely identified in the high-throughput sequencing. m6A-label-seq provides a strategy to label and identify cellular epitranscriptomic modification sites.For complete details on the use and execution of this profile, please refer to Shu et al., 2020.
abstract_unstemmed	Summary: We describe here a metabolic labeling protocol for detecting cellular transcriptome-wide mRNA N6-methyladenosine (m6A) at base resolution. By feeding cells an analog of methionine, potential mRNA m6A forming sites are replaced with the N6-allyladenosine (a6A). A mild chemical iodination of a6A in RNA results in its opposite base misincorporation during RNA reverse transcription, and thus m6A locations could be precisely identified in the high-throughput sequencing. m6A-label-seq provides a strategy to label and identify cellular epitranscriptomic modification sites.For complete details on the use and execution of this profile, please refer to Shu et al., 2020.
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title_short	m6A-label-seq: A metabolic labeling protocol to detect transcriptome-wide mRNA N6-methyladenosine (m6A) at base resolution
url	https://doi.org/10.1016/j.xpro.2021.101096 https://doaj.org/article/a705763d4b0a4f1ea8a2184d10fa601b http://www.sciencedirect.com/science/article/pii/S2666166721008029 https://doaj.org/toc/2666-1667
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m6A-label-seq: A metabolic labeling protocol to detect transcriptome-wide mRNA N6-methyladenosine (m6A) at base resolution

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