The Protective Effects of Origanum vulgare L. Extract on Genetic Damage of Cyclophosphamide in Mice Blood Lymphocytes Using Micronucleus Test

Background: Despite its various clinical applications, cyclophosphamide (CP), an alkylating chemotherapeutic agent, has demonstrated numerous side effects, including genetic toxicity. Objectives: This study investigated the protective action of Origanum vulgare L., a powerful antioxidant plant, on t...
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Autor*in:	Mohammad Shokrzadeh [verfasserIn] Emran Habibi [verfasserIn] Amir Shadboorestan [verfasserIn] Aroona Chabra [verfasserIn] Amirhossein Ahmadi [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	origanum vulgare antioxidant cyclophosphamide oxidative stress genotoxicity micronucleus

Übergeordnetes Werk:	In: Pharmaceutical and Biomedical Research - Mazandaran University of Medical Sciences, 2017, 6(2020), 4, Seite 297-302
Übergeordnetes Werk:	volume:6 ; year:2020 ; number:4 ; pages:297-302

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.18502/pbr.v6i4.5116

Katalog-ID:	DOAJ047472227

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520			\|a Background: Despite its various clinical applications, cyclophosphamide (CP), an alkylating chemotherapeutic agent, has demonstrated numerous side effects, including genetic toxicity. Objectives: This study investigated the protective action of Origanum vulgare L., a powerful antioxidant plant, on the genotoxicity of CP in the mice blood lymphocytes. Methods: The mice were pre-treated orally with different doses of 50, 100, 200, or 400 mg/kg O. vulgare ethanolic extract once a day for 7 consecutive days. One hour after the final dose of O. vulgare, each animal received a single intraperitoneal administration of 200 mg/kg CP. After 24 hours, the preventive effect of O. vulgare was evaluated using an in vitro micronucleus (Mn) test in cytokinesis-blocked lymphocytes, which is a reliable genotoxicity test. All doses of O. vulgare caused significant reductions in the CP-induced Mn formation, which served as an indicator of DNA damage in the peripheral blood lymphocytes. Results: The total reduction of the Mn in binucleated lymphocytes were 67% and 75% for doses of 200 and 400 mg/kg of O. vulgare, respectively (P<0.001). The antioxidant plant demonstrated dose-dependent protective effects against CP-induced Mn formation and genotoxicity in the blood lymphocytes of the mice. O. vulgare can reduce the damage to DNA through its potent antioxidation activity and free radical scavenging properties. Conclusion: Since it is widely used as a safe herbal medicine for many diseases, O. vulgare could be used to relieve the adverse effects of cyclophosphamide, especially against the genetic damages of normal cells in patients undergoing chemotherapy.
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spelling	10.18502/pbr.v6i4.5116 doi (DE-627)DOAJ047472227 (DE-599)DOAJ3e8d64770e34410689989700f6509c03 DE-627 ger DE-627 rakwb eng RM1-950 Mohammad Shokrzadeh verfasserin aut The Protective Effects of Origanum vulgare L. Extract on Genetic Damage of Cyclophosphamide in Mice Blood Lymphocytes Using Micronucleus Test 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Despite its various clinical applications, cyclophosphamide (CP), an alkylating chemotherapeutic agent, has demonstrated numerous side effects, including genetic toxicity. Objectives: This study investigated the protective action of Origanum vulgare L., a powerful antioxidant plant, on the genotoxicity of CP in the mice blood lymphocytes. Methods: The mice were pre-treated orally with different doses of 50, 100, 200, or 400 mg/kg O. vulgare ethanolic extract once a day for 7 consecutive days. One hour after the final dose of O. vulgare, each animal received a single intraperitoneal administration of 200 mg/kg CP. After 24 hours, the preventive effect of O. vulgare was evaluated using an in vitro micronucleus (Mn) test in cytokinesis-blocked lymphocytes, which is a reliable genotoxicity test. All doses of O. vulgare caused significant reductions in the CP-induced Mn formation, which served as an indicator of DNA damage in the peripheral blood lymphocytes. Results: The total reduction of the Mn in binucleated lymphocytes were 67% and 75% for doses of 200 and 400 mg/kg of O. vulgare, respectively (P<0.001). The antioxidant plant demonstrated dose-dependent protective effects against CP-induced Mn formation and genotoxicity in the blood lymphocytes of the mice. O. vulgare can reduce the damage to DNA through its potent antioxidation activity and free radical scavenging properties. Conclusion: Since it is widely used as a safe herbal medicine for many diseases, O. vulgare could be used to relieve the adverse effects of cyclophosphamide, especially against the genetic damages of normal cells in patients undergoing chemotherapy. origanum vulgare antioxidant cyclophosphamide oxidative stress genotoxicity micronucleus Therapeutics. Pharmacology Emran Habibi verfasserin aut Amir Shadboorestan verfasserin aut Aroona Chabra verfasserin aut Amirhossein Ahmadi verfasserin aut In Pharmaceutical and Biomedical Research Mazandaran University of Medical Sciences, 2017 6(2020), 4, Seite 297-302 (DE-627)845527452 (DE-600)2843863-2 24234494 nnns volume:6 year:2020 number:4 pages:297-302 https://doi.org/10.18502/pbr.v6i4.5116 kostenfrei https://doaj.org/article/3e8d64770e34410689989700f6509c03 kostenfrei http://pbr.mazums.ac.ir/article-1-257-en.html kostenfrei https://doaj.org/toc/2423-4494 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2020 4 297-302
allfields_unstemmed	10.18502/pbr.v6i4.5116 doi (DE-627)DOAJ047472227 (DE-599)DOAJ3e8d64770e34410689989700f6509c03 DE-627 ger DE-627 rakwb eng RM1-950 Mohammad Shokrzadeh verfasserin aut The Protective Effects of Origanum vulgare L. Extract on Genetic Damage of Cyclophosphamide in Mice Blood Lymphocytes Using Micronucleus Test 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Despite its various clinical applications, cyclophosphamide (CP), an alkylating chemotherapeutic agent, has demonstrated numerous side effects, including genetic toxicity. Objectives: This study investigated the protective action of Origanum vulgare L., a powerful antioxidant plant, on the genotoxicity of CP in the mice blood lymphocytes. Methods: The mice were pre-treated orally with different doses of 50, 100, 200, or 400 mg/kg O. vulgare ethanolic extract once a day for 7 consecutive days. One hour after the final dose of O. vulgare, each animal received a single intraperitoneal administration of 200 mg/kg CP. After 24 hours, the preventive effect of O. vulgare was evaluated using an in vitro micronucleus (Mn) test in cytokinesis-blocked lymphocytes, which is a reliable genotoxicity test. All doses of O. vulgare caused significant reductions in the CP-induced Mn formation, which served as an indicator of DNA damage in the peripheral blood lymphocytes. Results: The total reduction of the Mn in binucleated lymphocytes were 67% and 75% for doses of 200 and 400 mg/kg of O. vulgare, respectively (P<0.001). The antioxidant plant demonstrated dose-dependent protective effects against CP-induced Mn formation and genotoxicity in the blood lymphocytes of the mice. O. vulgare can reduce the damage to DNA through its potent antioxidation activity and free radical scavenging properties. Conclusion: Since it is widely used as a safe herbal medicine for many diseases, O. vulgare could be used to relieve the adverse effects of cyclophosphamide, especially against the genetic damages of normal cells in patients undergoing chemotherapy. origanum vulgare antioxidant cyclophosphamide oxidative stress genotoxicity micronucleus Therapeutics. Pharmacology Emran Habibi verfasserin aut Amir Shadboorestan verfasserin aut Aroona Chabra verfasserin aut Amirhossein Ahmadi verfasserin aut In Pharmaceutical and Biomedical Research Mazandaran University of Medical Sciences, 2017 6(2020), 4, Seite 297-302 (DE-627)845527452 (DE-600)2843863-2 24234494 nnns volume:6 year:2020 number:4 pages:297-302 https://doi.org/10.18502/pbr.v6i4.5116 kostenfrei https://doaj.org/article/3e8d64770e34410689989700f6509c03 kostenfrei http://pbr.mazums.ac.ir/article-1-257-en.html kostenfrei https://doaj.org/toc/2423-4494 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2020 4 297-302
allfieldsGer	10.18502/pbr.v6i4.5116 doi (DE-627)DOAJ047472227 (DE-599)DOAJ3e8d64770e34410689989700f6509c03 DE-627 ger DE-627 rakwb eng RM1-950 Mohammad Shokrzadeh verfasserin aut The Protective Effects of Origanum vulgare L. Extract on Genetic Damage of Cyclophosphamide in Mice Blood Lymphocytes Using Micronucleus Test 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Despite its various clinical applications, cyclophosphamide (CP), an alkylating chemotherapeutic agent, has demonstrated numerous side effects, including genetic toxicity. Objectives: This study investigated the protective action of Origanum vulgare L., a powerful antioxidant plant, on the genotoxicity of CP in the mice blood lymphocytes. Methods: The mice were pre-treated orally with different doses of 50, 100, 200, or 400 mg/kg O. vulgare ethanolic extract once a day for 7 consecutive days. One hour after the final dose of O. vulgare, each animal received a single intraperitoneal administration of 200 mg/kg CP. After 24 hours, the preventive effect of O. vulgare was evaluated using an in vitro micronucleus (Mn) test in cytokinesis-blocked lymphocytes, which is a reliable genotoxicity test. All doses of O. vulgare caused significant reductions in the CP-induced Mn formation, which served as an indicator of DNA damage in the peripheral blood lymphocytes. Results: The total reduction of the Mn in binucleated lymphocytes were 67% and 75% for doses of 200 and 400 mg/kg of O. vulgare, respectively (P<0.001). The antioxidant plant demonstrated dose-dependent protective effects against CP-induced Mn formation and genotoxicity in the blood lymphocytes of the mice. O. vulgare can reduce the damage to DNA through its potent antioxidation activity and free radical scavenging properties. Conclusion: Since it is widely used as a safe herbal medicine for many diseases, O. vulgare could be used to relieve the adverse effects of cyclophosphamide, especially against the genetic damages of normal cells in patients undergoing chemotherapy. origanum vulgare antioxidant cyclophosphamide oxidative stress genotoxicity micronucleus Therapeutics. Pharmacology Emran Habibi verfasserin aut Amir Shadboorestan verfasserin aut Aroona Chabra verfasserin aut Amirhossein Ahmadi verfasserin aut In Pharmaceutical and Biomedical Research Mazandaran University of Medical Sciences, 2017 6(2020), 4, Seite 297-302 (DE-627)845527452 (DE-600)2843863-2 24234494 nnns volume:6 year:2020 number:4 pages:297-302 https://doi.org/10.18502/pbr.v6i4.5116 kostenfrei https://doaj.org/article/3e8d64770e34410689989700f6509c03 kostenfrei http://pbr.mazums.ac.ir/article-1-257-en.html kostenfrei https://doaj.org/toc/2423-4494 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2020 4 297-302
allfieldsSound	10.18502/pbr.v6i4.5116 doi (DE-627)DOAJ047472227 (DE-599)DOAJ3e8d64770e34410689989700f6509c03 DE-627 ger DE-627 rakwb eng RM1-950 Mohammad Shokrzadeh verfasserin aut The Protective Effects of Origanum vulgare L. Extract on Genetic Damage of Cyclophosphamide in Mice Blood Lymphocytes Using Micronucleus Test 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Despite its various clinical applications, cyclophosphamide (CP), an alkylating chemotherapeutic agent, has demonstrated numerous side effects, including genetic toxicity. Objectives: This study investigated the protective action of Origanum vulgare L., a powerful antioxidant plant, on the genotoxicity of CP in the mice blood lymphocytes. Methods: The mice were pre-treated orally with different doses of 50, 100, 200, or 400 mg/kg O. vulgare ethanolic extract once a day for 7 consecutive days. One hour after the final dose of O. vulgare, each animal received a single intraperitoneal administration of 200 mg/kg CP. After 24 hours, the preventive effect of O. vulgare was evaluated using an in vitro micronucleus (Mn) test in cytokinesis-blocked lymphocytes, which is a reliable genotoxicity test. All doses of O. vulgare caused significant reductions in the CP-induced Mn formation, which served as an indicator of DNA damage in the peripheral blood lymphocytes. Results: The total reduction of the Mn in binucleated lymphocytes were 67% and 75% for doses of 200 and 400 mg/kg of O. vulgare, respectively (P<0.001). The antioxidant plant demonstrated dose-dependent protective effects against CP-induced Mn formation and genotoxicity in the blood lymphocytes of the mice. O. vulgare can reduce the damage to DNA through its potent antioxidation activity and free radical scavenging properties. Conclusion: Since it is widely used as a safe herbal medicine for many diseases, O. vulgare could be used to relieve the adverse effects of cyclophosphamide, especially against the genetic damages of normal cells in patients undergoing chemotherapy. origanum vulgare antioxidant cyclophosphamide oxidative stress genotoxicity micronucleus Therapeutics. Pharmacology Emran Habibi verfasserin aut Amir Shadboorestan verfasserin aut Aroona Chabra verfasserin aut Amirhossein Ahmadi verfasserin aut In Pharmaceutical and Biomedical Research Mazandaran University of Medical Sciences, 2017 6(2020), 4, Seite 297-302 (DE-627)845527452 (DE-600)2843863-2 24234494 nnns volume:6 year:2020 number:4 pages:297-302 https://doi.org/10.18502/pbr.v6i4.5116 kostenfrei https://doaj.org/article/3e8d64770e34410689989700f6509c03 kostenfrei http://pbr.mazums.ac.ir/article-1-257-en.html kostenfrei https://doaj.org/toc/2423-4494 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2020 4 297-302
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authorswithroles_txt_mv	Mohammad Shokrzadeh @@aut@@ Emran Habibi @@aut@@ Amir Shadboorestan @@aut@@ Aroona Chabra @@aut@@ Amirhossein Ahmadi @@aut@@
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author	Mohammad Shokrzadeh
spellingShingle	Mohammad Shokrzadeh misc RM1-950 misc origanum vulgare misc antioxidant misc cyclophosphamide misc oxidative stress misc genotoxicity misc micronucleus misc Therapeutics. Pharmacology The Protective Effects of Origanum vulgare L. Extract on Genetic Damage of Cyclophosphamide in Mice Blood Lymphocytes Using Micronucleus Test
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topic_title	RM1-950 The Protective Effects of Origanum vulgare L. Extract on Genetic Damage of Cyclophosphamide in Mice Blood Lymphocytes Using Micronucleus Test origanum vulgare antioxidant cyclophosphamide oxidative stress genotoxicity micronucleus
topic	misc RM1-950 misc origanum vulgare misc antioxidant misc cyclophosphamide misc oxidative stress misc genotoxicity misc micronucleus misc Therapeutics. Pharmacology
topic_unstemmed	misc RM1-950 misc origanum vulgare misc antioxidant misc cyclophosphamide misc oxidative stress misc genotoxicity misc micronucleus misc Therapeutics. Pharmacology
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title	The Protective Effects of Origanum vulgare L. Extract on Genetic Damage of Cyclophosphamide in Mice Blood Lymphocytes Using Micronucleus Test
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title_full	The Protective Effects of Origanum vulgare L. Extract on Genetic Damage of Cyclophosphamide in Mice Blood Lymphocytes Using Micronucleus Test
author_sort	Mohammad Shokrzadeh
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title_sort	protective effects of origanum vulgare l. extract on genetic damage of cyclophosphamide in mice blood lymphocytes using micronucleus test
callnumber	RM1-950
title_auth	The Protective Effects of Origanum vulgare L. Extract on Genetic Damage of Cyclophosphamide in Mice Blood Lymphocytes Using Micronucleus Test
abstract	Background: Despite its various clinical applications, cyclophosphamide (CP), an alkylating chemotherapeutic agent, has demonstrated numerous side effects, including genetic toxicity. Objectives: This study investigated the protective action of Origanum vulgare L., a powerful antioxidant plant, on the genotoxicity of CP in the mice blood lymphocytes. Methods: The mice were pre-treated orally with different doses of 50, 100, 200, or 400 mg/kg O. vulgare ethanolic extract once a day for 7 consecutive days. One hour after the final dose of O. vulgare, each animal received a single intraperitoneal administration of 200 mg/kg CP. After 24 hours, the preventive effect of O. vulgare was evaluated using an in vitro micronucleus (Mn) test in cytokinesis-blocked lymphocytes, which is a reliable genotoxicity test. All doses of O. vulgare caused significant reductions in the CP-induced Mn formation, which served as an indicator of DNA damage in the peripheral blood lymphocytes. Results: The total reduction of the Mn in binucleated lymphocytes were 67% and 75% for doses of 200 and 400 mg/kg of O. vulgare, respectively (P<0.001). The antioxidant plant demonstrated dose-dependent protective effects against CP-induced Mn formation and genotoxicity in the blood lymphocytes of the mice. O. vulgare can reduce the damage to DNA through its potent antioxidation activity and free radical scavenging properties. Conclusion: Since it is widely used as a safe herbal medicine for many diseases, O. vulgare could be used to relieve the adverse effects of cyclophosphamide, especially against the genetic damages of normal cells in patients undergoing chemotherapy.
abstractGer	Background: Despite its various clinical applications, cyclophosphamide (CP), an alkylating chemotherapeutic agent, has demonstrated numerous side effects, including genetic toxicity. Objectives: This study investigated the protective action of Origanum vulgare L., a powerful antioxidant plant, on the genotoxicity of CP in the mice blood lymphocytes. Methods: The mice were pre-treated orally with different doses of 50, 100, 200, or 400 mg/kg O. vulgare ethanolic extract once a day for 7 consecutive days. One hour after the final dose of O. vulgare, each animal received a single intraperitoneal administration of 200 mg/kg CP. After 24 hours, the preventive effect of O. vulgare was evaluated using an in vitro micronucleus (Mn) test in cytokinesis-blocked lymphocytes, which is a reliable genotoxicity test. All doses of O. vulgare caused significant reductions in the CP-induced Mn formation, which served as an indicator of DNA damage in the peripheral blood lymphocytes. Results: The total reduction of the Mn in binucleated lymphocytes were 67% and 75% for doses of 200 and 400 mg/kg of O. vulgare, respectively (P<0.001). The antioxidant plant demonstrated dose-dependent protective effects against CP-induced Mn formation and genotoxicity in the blood lymphocytes of the mice. O. vulgare can reduce the damage to DNA through its potent antioxidation activity and free radical scavenging properties. Conclusion: Since it is widely used as a safe herbal medicine for many diseases, O. vulgare could be used to relieve the adverse effects of cyclophosphamide, especially against the genetic damages of normal cells in patients undergoing chemotherapy.
abstract_unstemmed	Background: Despite its various clinical applications, cyclophosphamide (CP), an alkylating chemotherapeutic agent, has demonstrated numerous side effects, including genetic toxicity. Objectives: This study investigated the protective action of Origanum vulgare L., a powerful antioxidant plant, on the genotoxicity of CP in the mice blood lymphocytes. Methods: The mice were pre-treated orally with different doses of 50, 100, 200, or 400 mg/kg O. vulgare ethanolic extract once a day for 7 consecutive days. One hour after the final dose of O. vulgare, each animal received a single intraperitoneal administration of 200 mg/kg CP. After 24 hours, the preventive effect of O. vulgare was evaluated using an in vitro micronucleus (Mn) test in cytokinesis-blocked lymphocytes, which is a reliable genotoxicity test. All doses of O. vulgare caused significant reductions in the CP-induced Mn formation, which served as an indicator of DNA damage in the peripheral blood lymphocytes. Results: The total reduction of the Mn in binucleated lymphocytes were 67% and 75% for doses of 200 and 400 mg/kg of O. vulgare, respectively (P<0.001). The antioxidant plant demonstrated dose-dependent protective effects against CP-induced Mn formation and genotoxicity in the blood lymphocytes of the mice. O. vulgare can reduce the damage to DNA through its potent antioxidation activity and free radical scavenging properties. Conclusion: Since it is widely used as a safe herbal medicine for many diseases, O. vulgare could be used to relieve the adverse effects of cyclophosphamide, especially against the genetic damages of normal cells in patients undergoing chemotherapy.
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title_short	The Protective Effects of Origanum vulgare L. Extract on Genetic Damage of Cyclophosphamide in Mice Blood Lymphocytes Using Micronucleus Test
url	https://doi.org/10.18502/pbr.v6i4.5116 https://doaj.org/article/3e8d64770e34410689989700f6509c03 http://pbr.mazums.ac.ir/article-1-257-en.html https://doaj.org/toc/2423-4494
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Extract on Genetic Damage of Cyclophosphamide in Mice Blood Lymphocytes Using Micronucleus Test</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2020</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Background: Despite its various clinical applications, cyclophosphamide (CP), an alkylating chemotherapeutic agent, has demonstrated numerous side effects, including genetic toxicity. 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The antioxidant plant demonstrated dose-dependent protective effects against CP-induced Mn formation and genotoxicity in the blood lymphocytes of the mice. O. vulgare can reduce the damage to DNA through its potent antioxidation activity and free radical scavenging properties. 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The Protective Effects of Origanum vulgare L. Extract on Genetic Damage of Cyclophosphamide in Mice Blood Lymphocytes Using Micronucleus Test

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