Role of Inositol-1,4,5-Trisphosphate Receptor in the Regulation of Calcium Transients in Neonatal Rat Ventricular Myocytes

inositol 1,4,5-trisphosphate receptor, Ca2+ transient, ventricular myocyte, ryanodine receptor, gap junction

Gespeichert in:

Autor*in:	Zheng Zeng [verfasserIn] Heping Zhang [verfasserIn] Na Lin [verfasserIn] Man Kang [verfasserIn] Yuanyuan Zheng [verfasserIn] Chen Li [verfasserIn] Pingxiang Xu [verfasserIn] Yongquan Wu [verfasserIn] Dali Luo [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2014

Übergeordnetes Werk:	In: Journal of Pharmacological Sciences - Elsevier, 2017, 126(2014), 1, Seite 37-46
Übergeordnetes Werk:	volume:126 ; year:2014 ; number:1 ; pages:37-46

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1254/jphs.14029FP

Katalog-ID:	DOAJ047733497

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520			\|a This study determined the regulatory effect of inositol 1,4,5-trisphosphate receptors (IP3Rs) on the basal Ca2+ transients in cardiomyocytes. In cultured neonatal rat ventricular myocytes (NRVMs) at different densities, we used confocal microscopy to assess the effect of IP3Rs on the endogenous spontaneous Ca2+ oscillations through specific activation of IP3Rs with myo-IP3 hexakis (butyryloxymethyl) ester (IP3BM), a membrane permeable IP3, and interference of IP3R expression with shRNA. We found that NRVMs at the monolayer state displayed coordinated Ca2+ transients with less rate, shorter duration, and higher amplitude compared to single NRVMs. In addition, monolayer NRVMs exhibited 4 or 10 times more increased Ca2+ transients in response to phenylephrine, an α-adrenergic receptor agonist, or IP3BM than single NRVMs did, while the transient pattern remained unaltered, suggesting that the sensitivity of intracellular Ca2+ response to IP3R activation is different between single and monolayer NRVMs. However, interference of IP3R expression with shRNA reduced the frequency and amplitude of the spontaneous Ca2+ fluctuates similarly in both densities of NRVMs, resembling the effects of ryanodine receptor inhibition by ryanodine or tetracaine. Our findings suggest that IP3Rs are involved, in part, in the regulation of native Ca2+ transients, in profiles of their initiation and Ca2+ release extent, in developing cardiomyocytes. In addition, caution should be paid in evaluating the behavior of Ca2+ signaling in primary cultured cardiomyocytes at different densities. Keywords:: inositol 1,4,5-trisphosphate receptor, Ca2+ transient, ventricular myocyte, ryanodine receptor, gap junction
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allfields_unstemmed	10.1254/jphs.14029FP doi (DE-627)DOAJ047733497 (DE-599)DOAJ87f38789afc0476ba832ac6b32800cea DE-627 ger DE-627 rakwb eng RM1-950 Zheng Zeng verfasserin aut Role of Inositol-1,4,5-Trisphosphate Receptor in the Regulation of Calcium Transients in Neonatal Rat Ventricular Myocytes 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier This study determined the regulatory effect of inositol 1,4,5-trisphosphate receptors (IP3Rs) on the basal Ca2+ transients in cardiomyocytes. In cultured neonatal rat ventricular myocytes (NRVMs) at different densities, we used confocal microscopy to assess the effect of IP3Rs on the endogenous spontaneous Ca2+ oscillations through specific activation of IP3Rs with myo-IP3 hexakis (butyryloxymethyl) ester (IP3BM), a membrane permeable IP3, and interference of IP3R expression with shRNA. We found that NRVMs at the monolayer state displayed coordinated Ca2+ transients with less rate, shorter duration, and higher amplitude compared to single NRVMs. In addition, monolayer NRVMs exhibited 4 or 10 times more increased Ca2+ transients in response to phenylephrine, an α-adrenergic receptor agonist, or IP3BM than single NRVMs did, while the transient pattern remained unaltered, suggesting that the sensitivity of intracellular Ca2+ response to IP3R activation is different between single and monolayer NRVMs. However, interference of IP3R expression with shRNA reduced the frequency and amplitude of the spontaneous Ca2+ fluctuates similarly in both densities of NRVMs, resembling the effects of ryanodine receptor inhibition by ryanodine or tetracaine. Our findings suggest that IP3Rs are involved, in part, in the regulation of native Ca2+ transients, in profiles of their initiation and Ca2+ release extent, in developing cardiomyocytes. In addition, caution should be paid in evaluating the behavior of Ca2+ signaling in primary cultured cardiomyocytes at different densities. Keywords:: inositol 1,4,5-trisphosphate receptor, Ca2+ transient, ventricular myocyte, ryanodine receptor, gap junction Therapeutics. Pharmacology Heping Zhang verfasserin aut Na Lin verfasserin aut Man Kang verfasserin aut Yuanyuan Zheng verfasserin aut Chen Li verfasserin aut Pingxiang Xu verfasserin aut Yongquan Wu verfasserin aut Dali Luo verfasserin aut In Journal of Pharmacological Sciences Elsevier, 2017 126(2014), 1, Seite 37-46 (DE-627)1760631620 13478613 nnns volume:126 year:2014 number:1 pages:37-46 https://doi.org/10.1254/jphs.14029FP kostenfrei https://doaj.org/article/87f38789afc0476ba832ac6b32800cea kostenfrei http://www.sciencedirect.com/science/article/pii/S1347861319300453 kostenfrei https://doaj.org/toc/1347-8613 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 126 2014 1 37-46
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topic_title	RM1-950 Role of Inositol-1,4,5-Trisphosphate Receptor in the Regulation of Calcium Transients in Neonatal Rat Ventricular Myocytes
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title	Role of Inositol-1,4,5-Trisphosphate Receptor in the Regulation of Calcium Transients in Neonatal Rat Ventricular Myocytes
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title_full	Role of Inositol-1,4,5-Trisphosphate Receptor in the Regulation of Calcium Transients in Neonatal Rat Ventricular Myocytes
author_sort	Zheng Zeng
journal	Journal of Pharmacological Sciences
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author_browse	Zheng Zeng Heping Zhang Na Lin Man Kang Yuanyuan Zheng Chen Li Pingxiang Xu Yongquan Wu Dali Luo
container_volume	126
class	RM1-950
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doi_str_mv	10.1254/jphs.14029FP
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title_sort	role of inositol-1,4,5-trisphosphate receptor in the regulation of calcium transients in neonatal rat ventricular myocytes
callnumber	RM1-950
title_auth	Role of Inositol-1,4,5-Trisphosphate Receptor in the Regulation of Calcium Transients in Neonatal Rat Ventricular Myocytes
abstract	This study determined the regulatory effect of inositol 1,4,5-trisphosphate receptors (IP3Rs) on the basal Ca2+ transients in cardiomyocytes. In cultured neonatal rat ventricular myocytes (NRVMs) at different densities, we used confocal microscopy to assess the effect of IP3Rs on the endogenous spontaneous Ca2+ oscillations through specific activation of IP3Rs with myo-IP3 hexakis (butyryloxymethyl) ester (IP3BM), a membrane permeable IP3, and interference of IP3R expression with shRNA. We found that NRVMs at the monolayer state displayed coordinated Ca2+ transients with less rate, shorter duration, and higher amplitude compared to single NRVMs. In addition, monolayer NRVMs exhibited 4 or 10 times more increased Ca2+ transients in response to phenylephrine, an α-adrenergic receptor agonist, or IP3BM than single NRVMs did, while the transient pattern remained unaltered, suggesting that the sensitivity of intracellular Ca2+ response to IP3R activation is different between single and monolayer NRVMs. However, interference of IP3R expression with shRNA reduced the frequency and amplitude of the spontaneous Ca2+ fluctuates similarly in both densities of NRVMs, resembling the effects of ryanodine receptor inhibition by ryanodine or tetracaine. Our findings suggest that IP3Rs are involved, in part, in the regulation of native Ca2+ transients, in profiles of their initiation and Ca2+ release extent, in developing cardiomyocytes. In addition, caution should be paid in evaluating the behavior of Ca2+ signaling in primary cultured cardiomyocytes at different densities. Keywords:: inositol 1,4,5-trisphosphate receptor, Ca2+ transient, ventricular myocyte, ryanodine receptor, gap junction
abstractGer	This study determined the regulatory effect of inositol 1,4,5-trisphosphate receptors (IP3Rs) on the basal Ca2+ transients in cardiomyocytes. In cultured neonatal rat ventricular myocytes (NRVMs) at different densities, we used confocal microscopy to assess the effect of IP3Rs on the endogenous spontaneous Ca2+ oscillations through specific activation of IP3Rs with myo-IP3 hexakis (butyryloxymethyl) ester (IP3BM), a membrane permeable IP3, and interference of IP3R expression with shRNA. We found that NRVMs at the monolayer state displayed coordinated Ca2+ transients with less rate, shorter duration, and higher amplitude compared to single NRVMs. In addition, monolayer NRVMs exhibited 4 or 10 times more increased Ca2+ transients in response to phenylephrine, an α-adrenergic receptor agonist, or IP3BM than single NRVMs did, while the transient pattern remained unaltered, suggesting that the sensitivity of intracellular Ca2+ response to IP3R activation is different between single and monolayer NRVMs. However, interference of IP3R expression with shRNA reduced the frequency and amplitude of the spontaneous Ca2+ fluctuates similarly in both densities of NRVMs, resembling the effects of ryanodine receptor inhibition by ryanodine or tetracaine. Our findings suggest that IP3Rs are involved, in part, in the regulation of native Ca2+ transients, in profiles of their initiation and Ca2+ release extent, in developing cardiomyocytes. In addition, caution should be paid in evaluating the behavior of Ca2+ signaling in primary cultured cardiomyocytes at different densities. Keywords:: inositol 1,4,5-trisphosphate receptor, Ca2+ transient, ventricular myocyte, ryanodine receptor, gap junction
abstract_unstemmed	This study determined the regulatory effect of inositol 1,4,5-trisphosphate receptors (IP3Rs) on the basal Ca2+ transients in cardiomyocytes. In cultured neonatal rat ventricular myocytes (NRVMs) at different densities, we used confocal microscopy to assess the effect of IP3Rs on the endogenous spontaneous Ca2+ oscillations through specific activation of IP3Rs with myo-IP3 hexakis (butyryloxymethyl) ester (IP3BM), a membrane permeable IP3, and interference of IP3R expression with shRNA. We found that NRVMs at the monolayer state displayed coordinated Ca2+ transients with less rate, shorter duration, and higher amplitude compared to single NRVMs. In addition, monolayer NRVMs exhibited 4 or 10 times more increased Ca2+ transients in response to phenylephrine, an α-adrenergic receptor agonist, or IP3BM than single NRVMs did, while the transient pattern remained unaltered, suggesting that the sensitivity of intracellular Ca2+ response to IP3R activation is different between single and monolayer NRVMs. However, interference of IP3R expression with shRNA reduced the frequency and amplitude of the spontaneous Ca2+ fluctuates similarly in both densities of NRVMs, resembling the effects of ryanodine receptor inhibition by ryanodine or tetracaine. Our findings suggest that IP3Rs are involved, in part, in the regulation of native Ca2+ transients, in profiles of their initiation and Ca2+ release extent, in developing cardiomyocytes. In addition, caution should be paid in evaluating the behavior of Ca2+ signaling in primary cultured cardiomyocytes at different densities. Keywords:: inositol 1,4,5-trisphosphate receptor, Ca2+ transient, ventricular myocyte, ryanodine receptor, gap junction
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title_short	Role of Inositol-1,4,5-Trisphosphate Receptor in the Regulation of Calcium Transients in Neonatal Rat Ventricular Myocytes
url	https://doi.org/10.1254/jphs.14029FP https://doaj.org/article/87f38789afc0476ba832ac6b32800cea http://www.sciencedirect.com/science/article/pii/S1347861319300453 https://doaj.org/toc/1347-8613
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author2	Heping Zhang Na Lin Man Kang Yuanyuan Zheng Chen Li Pingxiang Xu Yongquan Wu Dali Luo
author2Str	Heping Zhang Na Lin Man Kang Yuanyuan Zheng Chen Li Pingxiang Xu Yongquan Wu Dali Luo
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doi_str	10.1254/jphs.14029FP
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up_date	2024-07-03T13:48:56.859Z
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Role of Inositol-1,4,5-Trisphosphate Receptor in the Regulation of Calcium Transients in Neonatal Rat Ventricular Myocytes

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