Identification of Genes Related to Paulownia Witches’ Broom by AFLP and MSAP

DNA methylation is believed to play important roles in regulating gene expression in plant growth and development. Paulownia witches’ broom (PaWB) infection has been reported to be related to gene expression changes in paulownia plantlets. To determine whether DNA methylation is associated with gen...
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Gespeichert in:

Autor*in:	Xibing Cao [verfasserIn] Guoqiang Fan [verfasserIn] Minjie Deng [verfasserIn] Zhenli Zhao [verfasserIn] Yanpeng Dong [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2014

Schlagwörter:	Paulownia tomentosa × Paulownia fortunei MMS DNA sequence DNA methylation AFLP MSAP polymorphism gene expression

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 15(2014), 8, Seite 14669-14683
Übergeordnetes Werk:	volume:15 ; year:2014 ; number:8 ; pages:14669-14683

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/ijms150814669

Katalog-ID:	DOAJ048174394

Internformat


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520			\|a DNA methylation is believed to play important roles in regulating gene expression in plant growth and development. Paulownia witches’ broom (PaWB) infection has been reported to be related to gene expression changes in paulownia plantlets. To determine whether DNA methylation is associated with gene expression changes in response to phytoplasma, we investigated variations in genomic DNA sequence and methylation in PaWB plantlets treated with methyl methane sulfonate (MMS) using amplified fragment length polymorphism (AFLP) and methylation-sensitive amplification polymorphism (MSAP) techniques, respectively. The results indicated that PaWB seedings recovered a normal morphology after treatment with more than 15 mg·L−1 MMS. PaWB infection did not cause changes of the paulownia DNA sequence at the AFLP level; However, DNA methylation levels and patterns were altered. Quantitative real-time PCR (qRT-PCR) showed that three of the methylated genes were up-regulated and three were down-regulated in the MMS-treated PaWB plantlets that had regained healthy morphology. These six genes might be involved in transcriptional regulation, plant defense, signal transduction and energy. The possible roles of these genes in PaWB are discussed. The results showed that changes of DNA methylation altered gene expression levels, and that MSAP might help identify genes related to PaWB.
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allfieldsSound	10.3390/ijms150814669 doi (DE-627)DOAJ048174394 (DE-599)DOAJedb5b443276d4e9598e1348b50d59e1e DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Xibing Cao verfasserin aut Identification of Genes Related to Paulownia Witches’ Broom by AFLP and MSAP 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier DNA methylation is believed to play important roles in regulating gene expression in plant growth and development. Paulownia witches’ broom (PaWB) infection has been reported to be related to gene expression changes in paulownia plantlets. To determine whether DNA methylation is associated with gene expression changes in response to phytoplasma, we investigated variations in genomic DNA sequence and methylation in PaWB plantlets treated with methyl methane sulfonate (MMS) using amplified fragment length polymorphism (AFLP) and methylation-sensitive amplification polymorphism (MSAP) techniques, respectively. The results indicated that PaWB seedings recovered a normal morphology after treatment with more than 15 mg·L−1 MMS. PaWB infection did not cause changes of the paulownia DNA sequence at the AFLP level; However, DNA methylation levels and patterns were altered. Quantitative real-time PCR (qRT-PCR) showed that three of the methylated genes were up-regulated and three were down-regulated in the MMS-treated PaWB plantlets that had regained healthy morphology. These six genes might be involved in transcriptional regulation, plant defense, signal transduction and energy. The possible roles of these genes in PaWB are discussed. The results showed that changes of DNA methylation altered gene expression levels, and that MSAP might help identify genes related to PaWB. Paulownia tomentosa × Paulownia fortunei MMS DNA sequence DNA methylation AFLP MSAP polymorphism gene expression Biology (General) Chemistry Guoqiang Fan verfasserin aut Minjie Deng verfasserin aut Zhenli Zhao verfasserin aut Yanpeng Dong verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 15(2014), 8, Seite 14669-14683 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:15 year:2014 number:8 pages:14669-14683 https://doi.org/10.3390/ijms150814669 kostenfrei https://doaj.org/article/edb5b443276d4e9598e1348b50d59e1e kostenfrei http://www.mdpi.com/1422-0067/15/8/14669 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 15 2014 8 14669-14683
language	English
source	In International Journal of Molecular Sciences 15(2014), 8, Seite 14669-14683 volume:15 year:2014 number:8 pages:14669-14683
sourceStr	In International Journal of Molecular Sciences 15(2014), 8, Seite 14669-14683 volume:15 year:2014 number:8 pages:14669-14683
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topic_facet	Paulownia tomentosa × Paulownia fortunei MMS DNA sequence DNA methylation AFLP MSAP polymorphism gene expression Biology (General) Chemistry
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container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Xibing Cao @@aut@@ Guoqiang Fan @@aut@@ Minjie Deng @@aut@@ Zhenli Zhao @@aut@@ Yanpeng Dong @@aut@@
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callnumber-first	Q - Science
author	Xibing Cao
spellingShingle	Xibing Cao misc QH301-705.5 misc QD1-999 misc Paulownia tomentosa × Paulownia fortunei misc MMS misc DNA sequence misc DNA methylation misc AFLP misc MSAP misc polymorphism misc gene expression misc Biology (General) misc Chemistry Identification of Genes Related to Paulownia Witches’ Broom by AFLP and MSAP
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topic_title	QH301-705.5 QD1-999 Identification of Genes Related to Paulownia Witches’ Broom by AFLP and MSAP Paulownia tomentosa × Paulownia fortunei MMS DNA sequence DNA methylation AFLP MSAP polymorphism gene expression
topic	misc QH301-705.5 misc QD1-999 misc Paulownia tomentosa × Paulownia fortunei misc MMS misc DNA sequence misc DNA methylation misc AFLP misc MSAP misc polymorphism misc gene expression misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc Paulownia tomentosa × Paulownia fortunei misc MMS misc DNA sequence misc DNA methylation misc AFLP misc MSAP misc polymorphism misc gene expression misc Biology (General) misc Chemistry
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title	Identification of Genes Related to Paulownia Witches’ Broom by AFLP and MSAP
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title_full	Identification of Genes Related to Paulownia Witches’ Broom by AFLP and MSAP
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title_sort	identification of genes related to paulownia witches’ broom by aflp and msap
callnumber	QH301-705.5
title_auth	Identification of Genes Related to Paulownia Witches’ Broom by AFLP and MSAP
abstract	DNA methylation is believed to play important roles in regulating gene expression in plant growth and development. Paulownia witches’ broom (PaWB) infection has been reported to be related to gene expression changes in paulownia plantlets. To determine whether DNA methylation is associated with gene expression changes in response to phytoplasma, we investigated variations in genomic DNA sequence and methylation in PaWB plantlets treated with methyl methane sulfonate (MMS) using amplified fragment length polymorphism (AFLP) and methylation-sensitive amplification polymorphism (MSAP) techniques, respectively. The results indicated that PaWB seedings recovered a normal morphology after treatment with more than 15 mg·L−1 MMS. PaWB infection did not cause changes of the paulownia DNA sequence at the AFLP level; However, DNA methylation levels and patterns were altered. Quantitative real-time PCR (qRT-PCR) showed that three of the methylated genes were up-regulated and three were down-regulated in the MMS-treated PaWB plantlets that had regained healthy morphology. These six genes might be involved in transcriptional regulation, plant defense, signal transduction and energy. The possible roles of these genes in PaWB are discussed. The results showed that changes of DNA methylation altered gene expression levels, and that MSAP might help identify genes related to PaWB.
abstractGer	DNA methylation is believed to play important roles in regulating gene expression in plant growth and development. Paulownia witches’ broom (PaWB) infection has been reported to be related to gene expression changes in paulownia plantlets. To determine whether DNA methylation is associated with gene expression changes in response to phytoplasma, we investigated variations in genomic DNA sequence and methylation in PaWB plantlets treated with methyl methane sulfonate (MMS) using amplified fragment length polymorphism (AFLP) and methylation-sensitive amplification polymorphism (MSAP) techniques, respectively. The results indicated that PaWB seedings recovered a normal morphology after treatment with more than 15 mg·L−1 MMS. PaWB infection did not cause changes of the paulownia DNA sequence at the AFLP level; However, DNA methylation levels and patterns were altered. Quantitative real-time PCR (qRT-PCR) showed that three of the methylated genes were up-regulated and three were down-regulated in the MMS-treated PaWB plantlets that had regained healthy morphology. These six genes might be involved in transcriptional regulation, plant defense, signal transduction and energy. The possible roles of these genes in PaWB are discussed. The results showed that changes of DNA methylation altered gene expression levels, and that MSAP might help identify genes related to PaWB.
abstract_unstemmed	DNA methylation is believed to play important roles in regulating gene expression in plant growth and development. Paulownia witches’ broom (PaWB) infection has been reported to be related to gene expression changes in paulownia plantlets. To determine whether DNA methylation is associated with gene expression changes in response to phytoplasma, we investigated variations in genomic DNA sequence and methylation in PaWB plantlets treated with methyl methane sulfonate (MMS) using amplified fragment length polymorphism (AFLP) and methylation-sensitive amplification polymorphism (MSAP) techniques, respectively. The results indicated that PaWB seedings recovered a normal morphology after treatment with more than 15 mg·L−1 MMS. PaWB infection did not cause changes of the paulownia DNA sequence at the AFLP level; However, DNA methylation levels and patterns were altered. Quantitative real-time PCR (qRT-PCR) showed that three of the methylated genes were up-regulated and three were down-regulated in the MMS-treated PaWB plantlets that had regained healthy morphology. These six genes might be involved in transcriptional regulation, plant defense, signal transduction and energy. The possible roles of these genes in PaWB are discussed. The results showed that changes of DNA methylation altered gene expression levels, and that MSAP might help identify genes related to PaWB.
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title_short	Identification of Genes Related to Paulownia Witches’ Broom by AFLP and MSAP
url	https://doi.org/10.3390/ijms150814669 https://doaj.org/article/edb5b443276d4e9598e1348b50d59e1e http://www.mdpi.com/1422-0067/15/8/14669 https://doaj.org/toc/1422-0067
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author2	Guoqiang Fan Minjie Deng Zhenli Zhao Yanpeng Dong
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up_date	2024-07-03T16:20:34.770Z
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These six genes might be involved in transcriptional regulation, plant defense, signal transduction and energy. The possible roles of these genes in PaWB are discussed. 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Identification of Genes Related to Paulownia Witches’ Broom by AFLP and MSAP

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