Non-Thermal Atmospheric Pressure Plasma-Conditioned Root Dentin Promotes Attraction and Attachment of Primary Human Dental Pulp Stem Cells in Real-Time Ex Vivo

This study investigated if non-thermal atmospheric pressure plasma (NTAPP) treatment of root dentin surfaces promotes human dental pulp stem cell (hDPSCs) adhesion. Freshly extracted human single-rooted teeth (<i<n</i< = 36) were decoronated and cut (first vertically, then horizontally)...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Yeon-Jee Yoo [verfasserIn] Min-Ji Kang [verfasserIn] Hiran Perinpanayagam [verfasserIn] Joo-Cheol Park [verfasserIn] Seung-Ho Baek [verfasserIn] Kee-Yeon Kum [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	dentin human dental pulp stem cells live-cell imaging MTT assay non-thermal atmospheric pressure plasma scanning electron microscopy

Übergeordnetes Werk:	In: Applied Sciences - MDPI AG, 2012, 11(2021), 15, p 6836
Übergeordnetes Werk:	volume:11 ; year:2021 ; number:15, p 6836

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/app11156836

Katalog-ID:	DOAJ049029932

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spelling	10.3390/app11156836 doi (DE-627)DOAJ049029932 (DE-599)DOAJb439226c913d4c7ab94936260ee14003 DE-627 ger DE-627 rakwb eng TA1-2040 QH301-705.5 QC1-999 QD1-999 Yeon-Jee Yoo verfasserin aut Non-Thermal Atmospheric Pressure Plasma-Conditioned Root Dentin Promotes Attraction and Attachment of Primary Human Dental Pulp Stem Cells in Real-Time Ex Vivo 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier This study investigated if non-thermal atmospheric pressure plasma (NTAPP) treatment of root dentin surfaces promotes human dental pulp stem cell (hDPSCs) adhesion. Freshly extracted human single-rooted teeth (<i<n</i< = 36) were decoronated and cut (first vertically, then horizontally) into root dentin slices (3 mm thick). Primary hDPSCs cultures were seeded onto slices randomly assigned to pretreatment groups (<i<n</i< = 9/group): NaOCl (1.5%), EDTA (17%) then NTAPP (Group I); NaOCl then NTAPP (Group II); NaOCl then EDTA (Group III); and NaOCl alone (Group IV). Cell viability and proliferation were measured using MTT assay with log-linear statistical analysis. Cell attachment and spreading morphologies on dentin slices (<i<n</i< = 3/group) were examined through scanning electron microscopy. Early cell adhesion events and subcellular activities were observed in real time by live-cell imaging through holotomographic microscopy. Cell viability and proliferation were significantly higher on NTAPP-treated dentin (<i<p</i< < 0.05), without interactions with EDTA (<i<p</i< < 0.05). The attachment, spreading, extensions and multiple layers of hDPSCs were heightened on NTAPP-treated dentin. Cell adhesion, spreading, and dentinal tubule penetration were hastened on NTAPP-treated dentin surfaces in real-time, with elevated subcellular activities and intracellular lipid droplet formation. NTAPP-treated root dentin surfaces support enhanced cellular responses, potentially promoting pulp-dentin regeneration. dentin human dental pulp stem cells live-cell imaging MTT assay non-thermal atmospheric pressure plasma scanning electron microscopy Technology T Engineering (General). Civil engineering (General) Biology (General) Physics Chemistry Min-Ji Kang verfasserin aut Hiran Perinpanayagam verfasserin aut Joo-Cheol Park verfasserin aut Seung-Ho Baek verfasserin aut Kee-Yeon Kum verfasserin aut In Applied Sciences MDPI AG, 2012 11(2021), 15, p 6836 (DE-627)737287640 (DE-600)2704225-X 20763417 nnns volume:11 year:2021 number:15, p 6836 https://doi.org/10.3390/app11156836 kostenfrei https://doaj.org/article/b439226c913d4c7ab94936260ee14003 kostenfrei https://www.mdpi.com/2076-3417/11/15/6836 kostenfrei https://doaj.org/toc/2076-3417 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2021 15, p 6836
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allfieldsSound	10.3390/app11156836 doi (DE-627)DOAJ049029932 (DE-599)DOAJb439226c913d4c7ab94936260ee14003 DE-627 ger DE-627 rakwb eng TA1-2040 QH301-705.5 QC1-999 QD1-999 Yeon-Jee Yoo verfasserin aut Non-Thermal Atmospheric Pressure Plasma-Conditioned Root Dentin Promotes Attraction and Attachment of Primary Human Dental Pulp Stem Cells in Real-Time Ex Vivo 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier This study investigated if non-thermal atmospheric pressure plasma (NTAPP) treatment of root dentin surfaces promotes human dental pulp stem cell (hDPSCs) adhesion. Freshly extracted human single-rooted teeth (<i<n</i< = 36) were decoronated and cut (first vertically, then horizontally) into root dentin slices (3 mm thick). Primary hDPSCs cultures were seeded onto slices randomly assigned to pretreatment groups (<i<n</i< = 9/group): NaOCl (1.5%), EDTA (17%) then NTAPP (Group I); NaOCl then NTAPP (Group II); NaOCl then EDTA (Group III); and NaOCl alone (Group IV). Cell viability and proliferation were measured using MTT assay with log-linear statistical analysis. Cell attachment and spreading morphologies on dentin slices (<i<n</i< = 3/group) were examined through scanning electron microscopy. Early cell adhesion events and subcellular activities were observed in real time by live-cell imaging through holotomographic microscopy. Cell viability and proliferation were significantly higher on NTAPP-treated dentin (<i<p</i< < 0.05), without interactions with EDTA (<i<p</i< < 0.05). The attachment, spreading, extensions and multiple layers of hDPSCs were heightened on NTAPP-treated dentin. Cell adhesion, spreading, and dentinal tubule penetration were hastened on NTAPP-treated dentin surfaces in real-time, with elevated subcellular activities and intracellular lipid droplet formation. NTAPP-treated root dentin surfaces support enhanced cellular responses, potentially promoting pulp-dentin regeneration. dentin human dental pulp stem cells live-cell imaging MTT assay non-thermal atmospheric pressure plasma scanning electron microscopy Technology T Engineering (General). Civil engineering (General) Biology (General) Physics Chemistry Min-Ji Kang verfasserin aut Hiran Perinpanayagam verfasserin aut Joo-Cheol Park verfasserin aut Seung-Ho Baek verfasserin aut Kee-Yeon Kum verfasserin aut In Applied Sciences MDPI AG, 2012 11(2021), 15, p 6836 (DE-627)737287640 (DE-600)2704225-X 20763417 nnns volume:11 year:2021 number:15, p 6836 https://doi.org/10.3390/app11156836 kostenfrei https://doaj.org/article/b439226c913d4c7ab94936260ee14003 kostenfrei https://www.mdpi.com/2076-3417/11/15/6836 kostenfrei https://doaj.org/toc/2076-3417 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2021 15, p 6836
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topic_facet	dentin human dental pulp stem cells live-cell imaging MTT assay non-thermal atmospheric pressure plasma scanning electron microscopy Technology T Engineering (General). Civil engineering (General) Biology (General) Physics Chemistry
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authorswithroles_txt_mv	Yeon-Jee Yoo @@aut@@ Min-Ji Kang @@aut@@ Hiran Perinpanayagam @@aut@@ Joo-Cheol Park @@aut@@ Seung-Ho Baek @@aut@@ Kee-Yeon Kum @@aut@@
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callnumber-first	T - Technology
author	Yeon-Jee Yoo
spellingShingle	Yeon-Jee Yoo misc TA1-2040 misc QH301-705.5 misc QC1-999 misc QD1-999 misc dentin misc human dental pulp stem cells misc live-cell imaging misc MTT assay misc non-thermal atmospheric pressure plasma misc scanning electron microscopy misc Technology misc T misc Engineering (General). Civil engineering (General) misc Biology (General) misc Physics misc Chemistry Non-Thermal Atmospheric Pressure Plasma-Conditioned Root Dentin Promotes Attraction and Attachment of Primary Human Dental Pulp Stem Cells in Real-Time Ex Vivo
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topic_title	TA1-2040 QH301-705.5 QC1-999 QD1-999 Non-Thermal Atmospheric Pressure Plasma-Conditioned Root Dentin Promotes Attraction and Attachment of Primary Human Dental Pulp Stem Cells in Real-Time Ex Vivo dentin human dental pulp stem cells live-cell imaging MTT assay non-thermal atmospheric pressure plasma scanning electron microscopy
topic	misc TA1-2040 misc QH301-705.5 misc QC1-999 misc QD1-999 misc dentin misc human dental pulp stem cells misc live-cell imaging misc MTT assay misc non-thermal atmospheric pressure plasma misc scanning electron microscopy misc Technology misc T misc Engineering (General). Civil engineering (General) misc Biology (General) misc Physics misc Chemistry
topic_unstemmed	misc TA1-2040 misc QH301-705.5 misc QC1-999 misc QD1-999 misc dentin misc human dental pulp stem cells misc live-cell imaging misc MTT assay misc non-thermal atmospheric pressure plasma misc scanning electron microscopy misc Technology misc T misc Engineering (General). Civil engineering (General) misc Biology (General) misc Physics misc Chemistry
topic_browse	misc TA1-2040 misc QH301-705.5 misc QC1-999 misc QD1-999 misc dentin misc human dental pulp stem cells misc live-cell imaging misc MTT assay misc non-thermal atmospheric pressure plasma misc scanning electron microscopy misc Technology misc T misc Engineering (General). Civil engineering (General) misc Biology (General) misc Physics misc Chemistry
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title	Non-Thermal Atmospheric Pressure Plasma-Conditioned Root Dentin Promotes Attraction and Attachment of Primary Human Dental Pulp Stem Cells in Real-Time Ex Vivo
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title_full	Non-Thermal Atmospheric Pressure Plasma-Conditioned Root Dentin Promotes Attraction and Attachment of Primary Human Dental Pulp Stem Cells in Real-Time Ex Vivo
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author_browse	Yeon-Jee Yoo Min-Ji Kang Hiran Perinpanayagam Joo-Cheol Park Seung-Ho Baek Kee-Yeon Kum
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title_sort	non-thermal atmospheric pressure plasma-conditioned root dentin promotes attraction and attachment of primary human dental pulp stem cells in real-time ex vivo
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title_auth	Non-Thermal Atmospheric Pressure Plasma-Conditioned Root Dentin Promotes Attraction and Attachment of Primary Human Dental Pulp Stem Cells in Real-Time Ex Vivo
abstract	This study investigated if non-thermal atmospheric pressure plasma (NTAPP) treatment of root dentin surfaces promotes human dental pulp stem cell (hDPSCs) adhesion. Freshly extracted human single-rooted teeth (<i<n</i< = 36) were decoronated and cut (first vertically, then horizontally) into root dentin slices (3 mm thick). Primary hDPSCs cultures were seeded onto slices randomly assigned to pretreatment groups (<i<n</i< = 9/group): NaOCl (1.5%), EDTA (17%) then NTAPP (Group I); NaOCl then NTAPP (Group II); NaOCl then EDTA (Group III); and NaOCl alone (Group IV). Cell viability and proliferation were measured using MTT assay with log-linear statistical analysis. Cell attachment and spreading morphologies on dentin slices (<i<n</i< = 3/group) were examined through scanning electron microscopy. Early cell adhesion events and subcellular activities were observed in real time by live-cell imaging through holotomographic microscopy. Cell viability and proliferation were significantly higher on NTAPP-treated dentin (<i<p</i< < 0.05), without interactions with EDTA (<i<p</i< < 0.05). The attachment, spreading, extensions and multiple layers of hDPSCs were heightened on NTAPP-treated dentin. Cell adhesion, spreading, and dentinal tubule penetration were hastened on NTAPP-treated dentin surfaces in real-time, with elevated subcellular activities and intracellular lipid droplet formation. NTAPP-treated root dentin surfaces support enhanced cellular responses, potentially promoting pulp-dentin regeneration.
abstractGer	This study investigated if non-thermal atmospheric pressure plasma (NTAPP) treatment of root dentin surfaces promotes human dental pulp stem cell (hDPSCs) adhesion. Freshly extracted human single-rooted teeth (<i<n</i< = 36) were decoronated and cut (first vertically, then horizontally) into root dentin slices (3 mm thick). Primary hDPSCs cultures were seeded onto slices randomly assigned to pretreatment groups (<i<n</i< = 9/group): NaOCl (1.5%), EDTA (17%) then NTAPP (Group I); NaOCl then NTAPP (Group II); NaOCl then EDTA (Group III); and NaOCl alone (Group IV). Cell viability and proliferation were measured using MTT assay with log-linear statistical analysis. Cell attachment and spreading morphologies on dentin slices (<i<n</i< = 3/group) were examined through scanning electron microscopy. Early cell adhesion events and subcellular activities were observed in real time by live-cell imaging through holotomographic microscopy. Cell viability and proliferation were significantly higher on NTAPP-treated dentin (<i<p</i< < 0.05), without interactions with EDTA (<i<p</i< < 0.05). The attachment, spreading, extensions and multiple layers of hDPSCs were heightened on NTAPP-treated dentin. Cell adhesion, spreading, and dentinal tubule penetration were hastened on NTAPP-treated dentin surfaces in real-time, with elevated subcellular activities and intracellular lipid droplet formation. NTAPP-treated root dentin surfaces support enhanced cellular responses, potentially promoting pulp-dentin regeneration.
abstract_unstemmed	This study investigated if non-thermal atmospheric pressure plasma (NTAPP) treatment of root dentin surfaces promotes human dental pulp stem cell (hDPSCs) adhesion. Freshly extracted human single-rooted teeth (<i<n</i< = 36) were decoronated and cut (first vertically, then horizontally) into root dentin slices (3 mm thick). Primary hDPSCs cultures were seeded onto slices randomly assigned to pretreatment groups (<i<n</i< = 9/group): NaOCl (1.5%), EDTA (17%) then NTAPP (Group I); NaOCl then NTAPP (Group II); NaOCl then EDTA (Group III); and NaOCl alone (Group IV). Cell viability and proliferation were measured using MTT assay with log-linear statistical analysis. Cell attachment and spreading morphologies on dentin slices (<i<n</i< = 3/group) were examined through scanning electron microscopy. Early cell adhesion events and subcellular activities were observed in real time by live-cell imaging through holotomographic microscopy. Cell viability and proliferation were significantly higher on NTAPP-treated dentin (<i<p</i< < 0.05), without interactions with EDTA (<i<p</i< < 0.05). The attachment, spreading, extensions and multiple layers of hDPSCs were heightened on NTAPP-treated dentin. Cell adhesion, spreading, and dentinal tubule penetration were hastened on NTAPP-treated dentin surfaces in real-time, with elevated subcellular activities and intracellular lipid droplet formation. NTAPP-treated root dentin surfaces support enhanced cellular responses, potentially promoting pulp-dentin regeneration.
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title_short	Non-Thermal Atmospheric Pressure Plasma-Conditioned Root Dentin Promotes Attraction and Attachment of Primary Human Dental Pulp Stem Cells in Real-Time Ex Vivo
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up_date	2024-07-03T21:00:22.918Z
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Freshly extracted human single-rooted teeth (<i<n</i< = 36) were decoronated and cut (first vertically, then horizontally) into root dentin slices (3 mm thick). Primary hDPSCs cultures were seeded onto slices randomly assigned to pretreatment groups (<i<n</i< = 9/group): NaOCl (1.5%), EDTA (17%) then NTAPP (Group I); NaOCl then NTAPP (Group II); NaOCl then EDTA (Group III); and NaOCl alone (Group IV). Cell viability and proliferation were measured using MTT assay with log-linear statistical analysis. Cell attachment and spreading morphologies on dentin slices (<i<n</i< = 3/group) were examined through scanning electron microscopy. Early cell adhesion events and subcellular activities were observed in real time by live-cell imaging through holotomographic microscopy. Cell viability and proliferation were significantly higher on NTAPP-treated dentin (<i<p</i< < 0.05), without interactions with EDTA (<i<p</i< < 0.05). 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Non-Thermal Atmospheric Pressure Plasma-Conditioned Root Dentin Promotes Attraction and Attachment of Primary Human Dental Pulp Stem Cells in Real-Time Ex Vivo

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