Transcriptomic Characterization of Nitrate-Enhanced Stevioside Glycoside Synthesis in Stevia (<i<Stevia rebaudiana</i<) Bertoni

Nitrogen forms (nitrate (NO<sub<3</sub<<sup<−</sup<) or ammonium (NH<sub<4</sub<<sup<+</sup<)) are vital to plant growth and metabolism. In stevia (<i<Stevia rebaudiana</i<), it is important to assess whether nitrogen forms can influence th...
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Autor*in:	Yuming Sun [verfasserIn] Ting Zhang [verfasserIn] Xiaoyang Xu [verfasserIn] Yongheng Yang [verfasserIn] Haiying Tong [verfasserIn] Luis Alejandro Jose Mur [verfasserIn] Haiyan Yuan [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	nitrogen forms transcriptome secondary metabolism transcription factors

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 22(2021), 16, p 8549
Übergeordnetes Werk:	volume:22 ; year:2021 ; number:16, p 8549

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms22168549

Katalog-ID:	DOAJ049068555

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allfields	10.3390/ijms22168549 doi (DE-627)DOAJ049068555 (DE-599)DOAJea933a2fbd8440ad8d08c1ce65755719 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Yuming Sun verfasserin aut Transcriptomic Characterization of Nitrate-Enhanced Stevioside Glycoside Synthesis in Stevia (<i<Stevia rebaudiana</i<) Bertoni 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Nitrogen forms (nitrate (NO<sub<3</sub<<sup<−</sup<) or ammonium (NH<sub<4</sub<<sup<+</sup<)) are vital to plant growth and metabolism. In stevia (<i<Stevia rebaudiana</i<), it is important to assess whether nitrogen forms can influence the synthesis of the high-value terpene metabolites-steviol glycosides (SGs), together with the underlying mechanisms. Field and pot experiments were performed where stevia plants were fertilized with either NO<sub<3</sub<<sup<−</sup< or NH<sub<4</sub<<sup<+</sup< nutrition to the same level of nitrogen. Physiological measurements suggested that nitrogen forms had no significant impact on biomass and the total nitrogen content of stevia leaves, but NO<sub<3</sub<<sup<−</sup<-enhanced leaf SGs contents. Transcriptomic analysis identified 397 genes that were differentially expressed (DEGs) between NO<sub<3</sub<<sup<−</sup< and NH<sub<4</sub<<sup<+</sup< treatments. Assessment of the DEGs highlighted the responses in secondary metabolism, particularly in terpenoid metabolism, to nitrogen forms. Further examinations of the expression patterns of SGs synthesis-related genes and potential transcription factors suggested that GGPPS and CPS genes, as well as the WRKY and MYB transcription factors, could be driving N form-regulated SG synthesis. We concluded that NO<sub<3</sub<<sup<−</sup<, rather than NH<sub<4</sub<<sup<+</sup<, can promote leaf SG synthesis via the NO<sub<3</sub<<sup<−</sup<-MYB/WRKY-GGPPS/CPS module. Our study suggests that insights into the molecular mechanism of how SG synthesis can be affected by nitrogen forms. <i<Stevia rebaudiana</i< nitrogen forms transcriptome secondary metabolism transcription factors Biology (General) Chemistry Ting Zhang verfasserin aut Xiaoyang Xu verfasserin aut Yongheng Yang verfasserin aut Haiying Tong verfasserin aut Luis Alejandro Jose Mur verfasserin aut Haiyan Yuan verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 16, p 8549 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:16, p 8549 https://doi.org/10.3390/ijms22168549 kostenfrei https://doaj.org/article/ea933a2fbd8440ad8d08c1ce65755719 kostenfrei https://www.mdpi.com/1422-0067/22/16/8549 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 16, p 8549
spelling	10.3390/ijms22168549 doi (DE-627)DOAJ049068555 (DE-599)DOAJea933a2fbd8440ad8d08c1ce65755719 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Yuming Sun verfasserin aut Transcriptomic Characterization of Nitrate-Enhanced Stevioside Glycoside Synthesis in Stevia (<i<Stevia rebaudiana</i<) Bertoni 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Nitrogen forms (nitrate (NO<sub<3</sub<<sup<−</sup<) or ammonium (NH<sub<4</sub<<sup<+</sup<)) are vital to plant growth and metabolism. In stevia (<i<Stevia rebaudiana</i<), it is important to assess whether nitrogen forms can influence the synthesis of the high-value terpene metabolites-steviol glycosides (SGs), together with the underlying mechanisms. Field and pot experiments were performed where stevia plants were fertilized with either NO<sub<3</sub<<sup<−</sup< or NH<sub<4</sub<<sup<+</sup< nutrition to the same level of nitrogen. Physiological measurements suggested that nitrogen forms had no significant impact on biomass and the total nitrogen content of stevia leaves, but NO<sub<3</sub<<sup<−</sup<-enhanced leaf SGs contents. Transcriptomic analysis identified 397 genes that were differentially expressed (DEGs) between NO<sub<3</sub<<sup<−</sup< and NH<sub<4</sub<<sup<+</sup< treatments. Assessment of the DEGs highlighted the responses in secondary metabolism, particularly in terpenoid metabolism, to nitrogen forms. Further examinations of the expression patterns of SGs synthesis-related genes and potential transcription factors suggested that GGPPS and CPS genes, as well as the WRKY and MYB transcription factors, could be driving N form-regulated SG synthesis. We concluded that NO<sub<3</sub<<sup<−</sup<, rather than NH<sub<4</sub<<sup<+</sup<, can promote leaf SG synthesis via the NO<sub<3</sub<<sup<−</sup<-MYB/WRKY-GGPPS/CPS module. Our study suggests that insights into the molecular mechanism of how SG synthesis can be affected by nitrogen forms. <i<Stevia rebaudiana</i< nitrogen forms transcriptome secondary metabolism transcription factors Biology (General) Chemistry Ting Zhang verfasserin aut Xiaoyang Xu verfasserin aut Yongheng Yang verfasserin aut Haiying Tong verfasserin aut Luis Alejandro Jose Mur verfasserin aut Haiyan Yuan verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 16, p 8549 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:16, p 8549 https://doi.org/10.3390/ijms22168549 kostenfrei https://doaj.org/article/ea933a2fbd8440ad8d08c1ce65755719 kostenfrei https://www.mdpi.com/1422-0067/22/16/8549 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 16, p 8549
allfields_unstemmed	10.3390/ijms22168549 doi (DE-627)DOAJ049068555 (DE-599)DOAJea933a2fbd8440ad8d08c1ce65755719 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Yuming Sun verfasserin aut Transcriptomic Characterization of Nitrate-Enhanced Stevioside Glycoside Synthesis in Stevia (<i<Stevia rebaudiana</i<) Bertoni 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Nitrogen forms (nitrate (NO<sub<3</sub<<sup<−</sup<) or ammonium (NH<sub<4</sub<<sup<+</sup<)) are vital to plant growth and metabolism. In stevia (<i<Stevia rebaudiana</i<), it is important to assess whether nitrogen forms can influence the synthesis of the high-value terpene metabolites-steviol glycosides (SGs), together with the underlying mechanisms. Field and pot experiments were performed where stevia plants were fertilized with either NO<sub<3</sub<<sup<−</sup< or NH<sub<4</sub<<sup<+</sup< nutrition to the same level of nitrogen. Physiological measurements suggested that nitrogen forms had no significant impact on biomass and the total nitrogen content of stevia leaves, but NO<sub<3</sub<<sup<−</sup<-enhanced leaf SGs contents. Transcriptomic analysis identified 397 genes that were differentially expressed (DEGs) between NO<sub<3</sub<<sup<−</sup< and NH<sub<4</sub<<sup<+</sup< treatments. Assessment of the DEGs highlighted the responses in secondary metabolism, particularly in terpenoid metabolism, to nitrogen forms. Further examinations of the expression patterns of SGs synthesis-related genes and potential transcription factors suggested that GGPPS and CPS genes, as well as the WRKY and MYB transcription factors, could be driving N form-regulated SG synthesis. We concluded that NO<sub<3</sub<<sup<−</sup<, rather than NH<sub<4</sub<<sup<+</sup<, can promote leaf SG synthesis via the NO<sub<3</sub<<sup<−</sup<-MYB/WRKY-GGPPS/CPS module. Our study suggests that insights into the molecular mechanism of how SG synthesis can be affected by nitrogen forms. <i<Stevia rebaudiana</i< nitrogen forms transcriptome secondary metabolism transcription factors Biology (General) Chemistry Ting Zhang verfasserin aut Xiaoyang Xu verfasserin aut Yongheng Yang verfasserin aut Haiying Tong verfasserin aut Luis Alejandro Jose Mur verfasserin aut Haiyan Yuan verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 16, p 8549 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:16, p 8549 https://doi.org/10.3390/ijms22168549 kostenfrei https://doaj.org/article/ea933a2fbd8440ad8d08c1ce65755719 kostenfrei https://www.mdpi.com/1422-0067/22/16/8549 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 16, p 8549
allfieldsGer	10.3390/ijms22168549 doi (DE-627)DOAJ049068555 (DE-599)DOAJea933a2fbd8440ad8d08c1ce65755719 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Yuming Sun verfasserin aut Transcriptomic Characterization of Nitrate-Enhanced Stevioside Glycoside Synthesis in Stevia (<i<Stevia rebaudiana</i<) Bertoni 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Nitrogen forms (nitrate (NO<sub<3</sub<<sup<−</sup<) or ammonium (NH<sub<4</sub<<sup<+</sup<)) are vital to plant growth and metabolism. In stevia (<i<Stevia rebaudiana</i<), it is important to assess whether nitrogen forms can influence the synthesis of the high-value terpene metabolites-steviol glycosides (SGs), together with the underlying mechanisms. Field and pot experiments were performed where stevia plants were fertilized with either NO<sub<3</sub<<sup<−</sup< or NH<sub<4</sub<<sup<+</sup< nutrition to the same level of nitrogen. Physiological measurements suggested that nitrogen forms had no significant impact on biomass and the total nitrogen content of stevia leaves, but NO<sub<3</sub<<sup<−</sup<-enhanced leaf SGs contents. Transcriptomic analysis identified 397 genes that were differentially expressed (DEGs) between NO<sub<3</sub<<sup<−</sup< and NH<sub<4</sub<<sup<+</sup< treatments. Assessment of the DEGs highlighted the responses in secondary metabolism, particularly in terpenoid metabolism, to nitrogen forms. Further examinations of the expression patterns of SGs synthesis-related genes and potential transcription factors suggested that GGPPS and CPS genes, as well as the WRKY and MYB transcription factors, could be driving N form-regulated SG synthesis. We concluded that NO<sub<3</sub<<sup<−</sup<, rather than NH<sub<4</sub<<sup<+</sup<, can promote leaf SG synthesis via the NO<sub<3</sub<<sup<−</sup<-MYB/WRKY-GGPPS/CPS module. Our study suggests that insights into the molecular mechanism of how SG synthesis can be affected by nitrogen forms. <i<Stevia rebaudiana</i< nitrogen forms transcriptome secondary metabolism transcription factors Biology (General) Chemistry Ting Zhang verfasserin aut Xiaoyang Xu verfasserin aut Yongheng Yang verfasserin aut Haiying Tong verfasserin aut Luis Alejandro Jose Mur verfasserin aut Haiyan Yuan verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 16, p 8549 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:16, p 8549 https://doi.org/10.3390/ijms22168549 kostenfrei https://doaj.org/article/ea933a2fbd8440ad8d08c1ce65755719 kostenfrei https://www.mdpi.com/1422-0067/22/16/8549 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 16, p 8549
allfieldsSound	10.3390/ijms22168549 doi (DE-627)DOAJ049068555 (DE-599)DOAJea933a2fbd8440ad8d08c1ce65755719 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Yuming Sun verfasserin aut Transcriptomic Characterization of Nitrate-Enhanced Stevioside Glycoside Synthesis in Stevia (<i<Stevia rebaudiana</i<) Bertoni 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Nitrogen forms (nitrate (NO<sub<3</sub<<sup<−</sup<) or ammonium (NH<sub<4</sub<<sup<+</sup<)) are vital to plant growth and metabolism. In stevia (<i<Stevia rebaudiana</i<), it is important to assess whether nitrogen forms can influence the synthesis of the high-value terpene metabolites-steviol glycosides (SGs), together with the underlying mechanisms. Field and pot experiments were performed where stevia plants were fertilized with either NO<sub<3</sub<<sup<−</sup< or NH<sub<4</sub<<sup<+</sup< nutrition to the same level of nitrogen. Physiological measurements suggested that nitrogen forms had no significant impact on biomass and the total nitrogen content of stevia leaves, but NO<sub<3</sub<<sup<−</sup<-enhanced leaf SGs contents. Transcriptomic analysis identified 397 genes that were differentially expressed (DEGs) between NO<sub<3</sub<<sup<−</sup< and NH<sub<4</sub<<sup<+</sup< treatments. Assessment of the DEGs highlighted the responses in secondary metabolism, particularly in terpenoid metabolism, to nitrogen forms. Further examinations of the expression patterns of SGs synthesis-related genes and potential transcription factors suggested that GGPPS and CPS genes, as well as the WRKY and MYB transcription factors, could be driving N form-regulated SG synthesis. We concluded that NO<sub<3</sub<<sup<−</sup<, rather than NH<sub<4</sub<<sup<+</sup<, can promote leaf SG synthesis via the NO<sub<3</sub<<sup<−</sup<-MYB/WRKY-GGPPS/CPS module. Our study suggests that insights into the molecular mechanism of how SG synthesis can be affected by nitrogen forms. <i<Stevia rebaudiana</i< nitrogen forms transcriptome secondary metabolism transcription factors Biology (General) Chemistry Ting Zhang verfasserin aut Xiaoyang Xu verfasserin aut Yongheng Yang verfasserin aut Haiying Tong verfasserin aut Luis Alejandro Jose Mur verfasserin aut Haiyan Yuan verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 16, p 8549 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:16, p 8549 https://doi.org/10.3390/ijms22168549 kostenfrei https://doaj.org/article/ea933a2fbd8440ad8d08c1ce65755719 kostenfrei https://www.mdpi.com/1422-0067/22/16/8549 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 16, p 8549
language	English
source	In International Journal of Molecular Sciences 22(2021), 16, p 8549 volume:22 year:2021 number:16, p 8549
sourceStr	In International Journal of Molecular Sciences 22(2021), 16, p 8549 volume:22 year:2021 number:16, p 8549
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	<i<Stevia rebaudiana</i< nitrogen forms transcriptome secondary metabolism transcription factors Biology (General) Chemistry
isfreeaccess_bool	true
container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Yuming Sun @@aut@@ Ting Zhang @@aut@@ Xiaoyang Xu @@aut@@ Yongheng Yang @@aut@@ Haiying Tong @@aut@@ Luis Alejandro Jose Mur @@aut@@ Haiyan Yuan @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	316340715
id	DOAJ049068555
language_de	englisch
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callnumber-first	Q - Science
author	Yuming Sun
spellingShingle	Yuming Sun misc QH301-705.5 misc QD1-999 misc <i<Stevia rebaudiana</i< misc nitrogen forms misc transcriptome misc secondary metabolism misc transcription factors misc Biology (General) misc Chemistry Transcriptomic Characterization of Nitrate-Enhanced Stevioside Glycoside Synthesis in Stevia (<i<Stevia rebaudiana</i<) Bertoni
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topic_title	QH301-705.5 QD1-999 Transcriptomic Characterization of Nitrate-Enhanced Stevioside Glycoside Synthesis in Stevia (<i<Stevia rebaudiana</i<) Bertoni <i<Stevia rebaudiana</i< nitrogen forms transcriptome secondary metabolism transcription factors
topic	misc QH301-705.5 misc QD1-999 misc <i<Stevia rebaudiana</i< misc nitrogen forms misc transcriptome misc secondary metabolism misc transcription factors misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc <i<Stevia rebaudiana</i< misc nitrogen forms misc transcriptome misc secondary metabolism misc transcription factors misc Biology (General) misc Chemistry
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title	Transcriptomic Characterization of Nitrate-Enhanced Stevioside Glycoside Synthesis in Stevia (<i<Stevia rebaudiana</i<) Bertoni
ctrlnum	(DE-627)DOAJ049068555 (DE-599)DOAJea933a2fbd8440ad8d08c1ce65755719
title_full	Transcriptomic Characterization of Nitrate-Enhanced Stevioside Glycoside Synthesis in Stevia (<i<Stevia rebaudiana</i<) Bertoni
author_sort	Yuming Sun
journal	International Journal of Molecular Sciences
journalStr	International Journal of Molecular Sciences
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author_browse	Yuming Sun Ting Zhang Xiaoyang Xu Yongheng Yang Haiying Tong Luis Alejandro Jose Mur Haiyan Yuan
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author-letter	Yuming Sun
doi_str_mv	10.3390/ijms22168549
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title_sort	transcriptomic characterization of nitrate-enhanced stevioside glycoside synthesis in stevia (<i<stevia rebaudiana</i<) bertoni
callnumber	QH301-705.5
title_auth	Transcriptomic Characterization of Nitrate-Enhanced Stevioside Glycoside Synthesis in Stevia (<i<Stevia rebaudiana</i<) Bertoni
abstract	Nitrogen forms (nitrate (NO<sub<3</sub<<sup<−</sup<) or ammonium (NH<sub<4</sub<<sup<+</sup<)) are vital to plant growth and metabolism. In stevia (<i<Stevia rebaudiana</i<), it is important to assess whether nitrogen forms can influence the synthesis of the high-value terpene metabolites-steviol glycosides (SGs), together with the underlying mechanisms. Field and pot experiments were performed where stevia plants were fertilized with either NO<sub<3</sub<<sup<−</sup< or NH<sub<4</sub<<sup<+</sup< nutrition to the same level of nitrogen. Physiological measurements suggested that nitrogen forms had no significant impact on biomass and the total nitrogen content of stevia leaves, but NO<sub<3</sub<<sup<−</sup<-enhanced leaf SGs contents. Transcriptomic analysis identified 397 genes that were differentially expressed (DEGs) between NO<sub<3</sub<<sup<−</sup< and NH<sub<4</sub<<sup<+</sup< treatments. Assessment of the DEGs highlighted the responses in secondary metabolism, particularly in terpenoid metabolism, to nitrogen forms. Further examinations of the expression patterns of SGs synthesis-related genes and potential transcription factors suggested that GGPPS and CPS genes, as well as the WRKY and MYB transcription factors, could be driving N form-regulated SG synthesis. We concluded that NO<sub<3</sub<<sup<−</sup<, rather than NH<sub<4</sub<<sup<+</sup<, can promote leaf SG synthesis via the NO<sub<3</sub<<sup<−</sup<-MYB/WRKY-GGPPS/CPS module. Our study suggests that insights into the molecular mechanism of how SG synthesis can be affected by nitrogen forms.
abstractGer	Nitrogen forms (nitrate (NO<sub<3</sub<<sup<−</sup<) or ammonium (NH<sub<4</sub<<sup<+</sup<)) are vital to plant growth and metabolism. In stevia (<i<Stevia rebaudiana</i<), it is important to assess whether nitrogen forms can influence the synthesis of the high-value terpene metabolites-steviol glycosides (SGs), together with the underlying mechanisms. Field and pot experiments were performed where stevia plants were fertilized with either NO<sub<3</sub<<sup<−</sup< or NH<sub<4</sub<<sup<+</sup< nutrition to the same level of nitrogen. Physiological measurements suggested that nitrogen forms had no significant impact on biomass and the total nitrogen content of stevia leaves, but NO<sub<3</sub<<sup<−</sup<-enhanced leaf SGs contents. Transcriptomic analysis identified 397 genes that were differentially expressed (DEGs) between NO<sub<3</sub<<sup<−</sup< and NH<sub<4</sub<<sup<+</sup< treatments. Assessment of the DEGs highlighted the responses in secondary metabolism, particularly in terpenoid metabolism, to nitrogen forms. Further examinations of the expression patterns of SGs synthesis-related genes and potential transcription factors suggested that GGPPS and CPS genes, as well as the WRKY and MYB transcription factors, could be driving N form-regulated SG synthesis. We concluded that NO<sub<3</sub<<sup<−</sup<, rather than NH<sub<4</sub<<sup<+</sup<, can promote leaf SG synthesis via the NO<sub<3</sub<<sup<−</sup<-MYB/WRKY-GGPPS/CPS module. Our study suggests that insights into the molecular mechanism of how SG synthesis can be affected by nitrogen forms.
abstract_unstemmed	Nitrogen forms (nitrate (NO<sub<3</sub<<sup<−</sup<) or ammonium (NH<sub<4</sub<<sup<+</sup<)) are vital to plant growth and metabolism. In stevia (<i<Stevia rebaudiana</i<), it is important to assess whether nitrogen forms can influence the synthesis of the high-value terpene metabolites-steviol glycosides (SGs), together with the underlying mechanisms. Field and pot experiments were performed where stevia plants were fertilized with either NO<sub<3</sub<<sup<−</sup< or NH<sub<4</sub<<sup<+</sup< nutrition to the same level of nitrogen. Physiological measurements suggested that nitrogen forms had no significant impact on biomass and the total nitrogen content of stevia leaves, but NO<sub<3</sub<<sup<−</sup<-enhanced leaf SGs contents. Transcriptomic analysis identified 397 genes that were differentially expressed (DEGs) between NO<sub<3</sub<<sup<−</sup< and NH<sub<4</sub<<sup<+</sup< treatments. Assessment of the DEGs highlighted the responses in secondary metabolism, particularly in terpenoid metabolism, to nitrogen forms. Further examinations of the expression patterns of SGs synthesis-related genes and potential transcription factors suggested that GGPPS and CPS genes, as well as the WRKY and MYB transcription factors, could be driving N form-regulated SG synthesis. We concluded that NO<sub<3</sub<<sup<−</sup<, rather than NH<sub<4</sub<<sup<+</sup<, can promote leaf SG synthesis via the NO<sub<3</sub<<sup<−</sup<-MYB/WRKY-GGPPS/CPS module. Our study suggests that insights into the molecular mechanism of how SG synthesis can be affected by nitrogen forms.
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title_short	Transcriptomic Characterization of Nitrate-Enhanced Stevioside Glycoside Synthesis in Stevia (<i<Stevia rebaudiana</i<) Bertoni
url	https://doi.org/10.3390/ijms22168549 https://doaj.org/article/ea933a2fbd8440ad8d08c1ce65755719 https://www.mdpi.com/1422-0067/22/16/8549 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067
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up_date	2024-07-03T21:14:22.956Z
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Transcriptomic Characterization of Nitrate-Enhanced Stevioside Glycoside Synthesis in Stevia (<i<Stevia rebaudiana</i<) Bertoni

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