An electrochemical aptasensor based on polythiophene-3-carboxylic acid assisted methylene blue for aflatoxin B1 detection

Aflatoxin B1 (AFB1) is the most toxic contaminant, causes carcinogenic, and immune toxic effects. There is an immense need to develop inexpensive, reliable biosensors for AFB1. The electrochemical aptasensor for detection of aflatoxin B1 has received much attention due to their trustworthy procedure...
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Autor*in:	Hanane Zejli [verfasserIn] K. Yugender Goud [verfasserIn] Jean Louis Marty [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Übergeordnetes Werk:	In: Sensing and Bio-Sensing Research - Elsevier, 2016, 25(2019), Seite -
Übergeordnetes Werk:	volume:25 ; year:2019 ; pages:-

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.sbsr.2019.100290

Katalog-ID:	DOAJ049466801

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245	1	3	\|a An electrochemical aptasensor based on polythiophene-3-carboxylic acid assisted methylene blue for aflatoxin B1 detection
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520			\|a Aflatoxin B1 (AFB1) is the most toxic contaminant, causes carcinogenic, and immune toxic effects. There is an immense need to develop inexpensive, reliable biosensors for AFB1. The electrochemical aptasensor for detection of aflatoxin B1 has received much attention due to their trustworthy procedure. In this work, we have designed an electrochemical aptasensor by using methylene blue (MB) as a signalling fragment and polythiophene-3-carboxylic acid (PT3C) as the signal-enlarging platform. The (PT3C) was first screened onto the screen printed carbon electrode (SPCE) interface, and then the MB-tagged aptamer was immobilized on SPCE by using hexamethylenediamine (HMDA) as a spacer via carbodiimide amide-bonding chemistry. AFB1 has been chosen as model analyte to test the proposed aptasensing platform. A good dynamic range 2.5–30 ng L−1 was obtained for AFB1 with a limit of detection (LOD 1.6 ng L−1). The good reproducibility was recorded with RSD% of 3.21. The obtained straight-line equation was 3.6099 x + 19.073, R2 = 0.9977. For real-world sample applications, this aptasensor has been used in coffee samples. It displayed good recovery in the range 88.2–93.3%, thus showed the effectiveness of the proposed aptasensor for coffee samples. Keywords: Aflatoxin B1, Polythiophene-3-carboxylic acid (PT3C), Screen-printed carbon electrodes, Aptamer, Coffee samples
653		0	\|a Engineering (General). Civil engineering (General)
700	0		\|a K. Yugender Goud \|e verfasserin \|4 aut
700	0		\|a Jean Louis Marty \|e verfasserin \|4 aut
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allfields	10.1016/j.sbsr.2019.100290 doi (DE-627)DOAJ049466801 (DE-599)DOAJ4a17ad5f7f5d4670a5714548bff15d4e DE-627 ger DE-627 rakwb eng TA1-2040 Hanane Zejli verfasserin aut An electrochemical aptasensor based on polythiophene-3-carboxylic acid assisted methylene blue for aflatoxin B1 detection 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aflatoxin B1 (AFB1) is the most toxic contaminant, causes carcinogenic, and immune toxic effects. There is an immense need to develop inexpensive, reliable biosensors for AFB1. The electrochemical aptasensor for detection of aflatoxin B1 has received much attention due to their trustworthy procedure. In this work, we have designed an electrochemical aptasensor by using methylene blue (MB) as a signalling fragment and polythiophene-3-carboxylic acid (PT3C) as the signal-enlarging platform. The (PT3C) was first screened onto the screen printed carbon electrode (SPCE) interface, and then the MB-tagged aptamer was immobilized on SPCE by using hexamethylenediamine (HMDA) as a spacer via carbodiimide amide-bonding chemistry. AFB1 has been chosen as model analyte to test the proposed aptasensing platform. A good dynamic range 2.5–30 ng L−1 was obtained for AFB1 with a limit of detection (LOD 1.6 ng L−1). The good reproducibility was recorded with RSD% of 3.21. The obtained straight-line equation was 3.6099 x + 19.073, R2 = 0.9977. For real-world sample applications, this aptasensor has been used in coffee samples. It displayed good recovery in the range 88.2–93.3%, thus showed the effectiveness of the proposed aptasensor for coffee samples. Keywords: Aflatoxin B1, Polythiophene-3-carboxylic acid (PT3C), Screen-printed carbon electrodes, Aptamer, Coffee samples Engineering (General). Civil engineering (General) K. Yugender Goud verfasserin aut Jean Louis Marty verfasserin aut In Sensing and Bio-Sensing Research Elsevier, 2016 25(2019), Seite - (DE-627)826105408 (DE-600)2821969-7 22141804 nnns volume:25 year:2019 pages:- https://doi.org/10.1016/j.sbsr.2019.100290 kostenfrei https://doaj.org/article/4a17ad5f7f5d4670a5714548bff15d4e kostenfrei http://www.sciencedirect.com/science/article/pii/S2214180419300480 kostenfrei https://doaj.org/toc/2214-1804 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 25 2019 -
spelling	10.1016/j.sbsr.2019.100290 doi (DE-627)DOAJ049466801 (DE-599)DOAJ4a17ad5f7f5d4670a5714548bff15d4e DE-627 ger DE-627 rakwb eng TA1-2040 Hanane Zejli verfasserin aut An electrochemical aptasensor based on polythiophene-3-carboxylic acid assisted methylene blue for aflatoxin B1 detection 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aflatoxin B1 (AFB1) is the most toxic contaminant, causes carcinogenic, and immune toxic effects. There is an immense need to develop inexpensive, reliable biosensors for AFB1. The electrochemical aptasensor for detection of aflatoxin B1 has received much attention due to their trustworthy procedure. In this work, we have designed an electrochemical aptasensor by using methylene blue (MB) as a signalling fragment and polythiophene-3-carboxylic acid (PT3C) as the signal-enlarging platform. The (PT3C) was first screened onto the screen printed carbon electrode (SPCE) interface, and then the MB-tagged aptamer was immobilized on SPCE by using hexamethylenediamine (HMDA) as a spacer via carbodiimide amide-bonding chemistry. AFB1 has been chosen as model analyte to test the proposed aptasensing platform. A good dynamic range 2.5–30 ng L−1 was obtained for AFB1 with a limit of detection (LOD 1.6 ng L−1). The good reproducibility was recorded with RSD% of 3.21. The obtained straight-line equation was 3.6099 x + 19.073, R2 = 0.9977. For real-world sample applications, this aptasensor has been used in coffee samples. It displayed good recovery in the range 88.2–93.3%, thus showed the effectiveness of the proposed aptasensor for coffee samples. Keywords: Aflatoxin B1, Polythiophene-3-carboxylic acid (PT3C), Screen-printed carbon electrodes, Aptamer, Coffee samples Engineering (General). Civil engineering (General) K. Yugender Goud verfasserin aut Jean Louis Marty verfasserin aut In Sensing and Bio-Sensing Research Elsevier, 2016 25(2019), Seite - (DE-627)826105408 (DE-600)2821969-7 22141804 nnns volume:25 year:2019 pages:- https://doi.org/10.1016/j.sbsr.2019.100290 kostenfrei https://doaj.org/article/4a17ad5f7f5d4670a5714548bff15d4e kostenfrei http://www.sciencedirect.com/science/article/pii/S2214180419300480 kostenfrei https://doaj.org/toc/2214-1804 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 25 2019 -
allfields_unstemmed	10.1016/j.sbsr.2019.100290 doi (DE-627)DOAJ049466801 (DE-599)DOAJ4a17ad5f7f5d4670a5714548bff15d4e DE-627 ger DE-627 rakwb eng TA1-2040 Hanane Zejli verfasserin aut An electrochemical aptasensor based on polythiophene-3-carboxylic acid assisted methylene blue for aflatoxin B1 detection 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aflatoxin B1 (AFB1) is the most toxic contaminant, causes carcinogenic, and immune toxic effects. There is an immense need to develop inexpensive, reliable biosensors for AFB1. The electrochemical aptasensor for detection of aflatoxin B1 has received much attention due to their trustworthy procedure. In this work, we have designed an electrochemical aptasensor by using methylene blue (MB) as a signalling fragment and polythiophene-3-carboxylic acid (PT3C) as the signal-enlarging platform. The (PT3C) was first screened onto the screen printed carbon electrode (SPCE) interface, and then the MB-tagged aptamer was immobilized on SPCE by using hexamethylenediamine (HMDA) as a spacer via carbodiimide amide-bonding chemistry. AFB1 has been chosen as model analyte to test the proposed aptasensing platform. A good dynamic range 2.5–30 ng L−1 was obtained for AFB1 with a limit of detection (LOD 1.6 ng L−1). The good reproducibility was recorded with RSD% of 3.21. The obtained straight-line equation was 3.6099 x + 19.073, R2 = 0.9977. For real-world sample applications, this aptasensor has been used in coffee samples. It displayed good recovery in the range 88.2–93.3%, thus showed the effectiveness of the proposed aptasensor for coffee samples. Keywords: Aflatoxin B1, Polythiophene-3-carboxylic acid (PT3C), Screen-printed carbon electrodes, Aptamer, Coffee samples Engineering (General). Civil engineering (General) K. Yugender Goud verfasserin aut Jean Louis Marty verfasserin aut In Sensing and Bio-Sensing Research Elsevier, 2016 25(2019), Seite - (DE-627)826105408 (DE-600)2821969-7 22141804 nnns volume:25 year:2019 pages:- https://doi.org/10.1016/j.sbsr.2019.100290 kostenfrei https://doaj.org/article/4a17ad5f7f5d4670a5714548bff15d4e kostenfrei http://www.sciencedirect.com/science/article/pii/S2214180419300480 kostenfrei https://doaj.org/toc/2214-1804 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 25 2019 -
allfieldsGer	10.1016/j.sbsr.2019.100290 doi (DE-627)DOAJ049466801 (DE-599)DOAJ4a17ad5f7f5d4670a5714548bff15d4e DE-627 ger DE-627 rakwb eng TA1-2040 Hanane Zejli verfasserin aut An electrochemical aptasensor based on polythiophene-3-carboxylic acid assisted methylene blue for aflatoxin B1 detection 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aflatoxin B1 (AFB1) is the most toxic contaminant, causes carcinogenic, and immune toxic effects. There is an immense need to develop inexpensive, reliable biosensors for AFB1. The electrochemical aptasensor for detection of aflatoxin B1 has received much attention due to their trustworthy procedure. In this work, we have designed an electrochemical aptasensor by using methylene blue (MB) as a signalling fragment and polythiophene-3-carboxylic acid (PT3C) as the signal-enlarging platform. The (PT3C) was first screened onto the screen printed carbon electrode (SPCE) interface, and then the MB-tagged aptamer was immobilized on SPCE by using hexamethylenediamine (HMDA) as a spacer via carbodiimide amide-bonding chemistry. AFB1 has been chosen as model analyte to test the proposed aptasensing platform. A good dynamic range 2.5–30 ng L−1 was obtained for AFB1 with a limit of detection (LOD 1.6 ng L−1). The good reproducibility was recorded with RSD% of 3.21. The obtained straight-line equation was 3.6099 x + 19.073, R2 = 0.9977. For real-world sample applications, this aptasensor has been used in coffee samples. It displayed good recovery in the range 88.2–93.3%, thus showed the effectiveness of the proposed aptasensor for coffee samples. Keywords: Aflatoxin B1, Polythiophene-3-carboxylic acid (PT3C), Screen-printed carbon electrodes, Aptamer, Coffee samples Engineering (General). Civil engineering (General) K. Yugender Goud verfasserin aut Jean Louis Marty verfasserin aut In Sensing and Bio-Sensing Research Elsevier, 2016 25(2019), Seite - (DE-627)826105408 (DE-600)2821969-7 22141804 nnns volume:25 year:2019 pages:- https://doi.org/10.1016/j.sbsr.2019.100290 kostenfrei https://doaj.org/article/4a17ad5f7f5d4670a5714548bff15d4e kostenfrei http://www.sciencedirect.com/science/article/pii/S2214180419300480 kostenfrei https://doaj.org/toc/2214-1804 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 25 2019 -
allfieldsSound	10.1016/j.sbsr.2019.100290 doi (DE-627)DOAJ049466801 (DE-599)DOAJ4a17ad5f7f5d4670a5714548bff15d4e DE-627 ger DE-627 rakwb eng TA1-2040 Hanane Zejli verfasserin aut An electrochemical aptasensor based on polythiophene-3-carboxylic acid assisted methylene blue for aflatoxin B1 detection 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aflatoxin B1 (AFB1) is the most toxic contaminant, causes carcinogenic, and immune toxic effects. There is an immense need to develop inexpensive, reliable biosensors for AFB1. The electrochemical aptasensor for detection of aflatoxin B1 has received much attention due to their trustworthy procedure. In this work, we have designed an electrochemical aptasensor by using methylene blue (MB) as a signalling fragment and polythiophene-3-carboxylic acid (PT3C) as the signal-enlarging platform. The (PT3C) was first screened onto the screen printed carbon electrode (SPCE) interface, and then the MB-tagged aptamer was immobilized on SPCE by using hexamethylenediamine (HMDA) as a spacer via carbodiimide amide-bonding chemistry. AFB1 has been chosen as model analyte to test the proposed aptasensing platform. A good dynamic range 2.5–30 ng L−1 was obtained for AFB1 with a limit of detection (LOD 1.6 ng L−1). The good reproducibility was recorded with RSD% of 3.21. The obtained straight-line equation was 3.6099 x + 19.073, R2 = 0.9977. For real-world sample applications, this aptasensor has been used in coffee samples. It displayed good recovery in the range 88.2–93.3%, thus showed the effectiveness of the proposed aptasensor for coffee samples. Keywords: Aflatoxin B1, Polythiophene-3-carboxylic acid (PT3C), Screen-printed carbon electrodes, Aptamer, Coffee samples Engineering (General). Civil engineering (General) K. Yugender Goud verfasserin aut Jean Louis Marty verfasserin aut In Sensing and Bio-Sensing Research Elsevier, 2016 25(2019), Seite - (DE-627)826105408 (DE-600)2821969-7 22141804 nnns volume:25 year:2019 pages:- https://doi.org/10.1016/j.sbsr.2019.100290 kostenfrei https://doaj.org/article/4a17ad5f7f5d4670a5714548bff15d4e kostenfrei http://www.sciencedirect.com/science/article/pii/S2214180419300480 kostenfrei https://doaj.org/toc/2214-1804 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 25 2019 -
language	English
source	In Sensing and Bio-Sensing Research 25(2019), Seite - volume:25 year:2019 pages:-
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container_title	Sensing and Bio-Sensing Research
authorswithroles_txt_mv	Hanane Zejli @@aut@@ K. Yugender Goud @@aut@@ Jean Louis Marty @@aut@@
publishDateDaySort_date	2019-01-01T00:00:00Z
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callnumber-first	T - Technology
author	Hanane Zejli
spellingShingle	Hanane Zejli misc TA1-2040 misc Engineering (General). Civil engineering (General) An electrochemical aptasensor based on polythiophene-3-carboxylic acid assisted methylene blue for aflatoxin B1 detection
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topic_title	TA1-2040 An electrochemical aptasensor based on polythiophene-3-carboxylic acid assisted methylene blue for aflatoxin B1 detection
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title	An electrochemical aptasensor based on polythiophene-3-carboxylic acid assisted methylene blue for aflatoxin B1 detection
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title_full	An electrochemical aptasensor based on polythiophene-3-carboxylic acid assisted methylene blue for aflatoxin B1 detection
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title_sort	electrochemical aptasensor based on polythiophene-3-carboxylic acid assisted methylene blue for aflatoxin b1 detection
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title_auth	An electrochemical aptasensor based on polythiophene-3-carboxylic acid assisted methylene blue for aflatoxin B1 detection
abstract	Aflatoxin B1 (AFB1) is the most toxic contaminant, causes carcinogenic, and immune toxic effects. There is an immense need to develop inexpensive, reliable biosensors for AFB1. The electrochemical aptasensor for detection of aflatoxin B1 has received much attention due to their trustworthy procedure. In this work, we have designed an electrochemical aptasensor by using methylene blue (MB) as a signalling fragment and polythiophene-3-carboxylic acid (PT3C) as the signal-enlarging platform. The (PT3C) was first screened onto the screen printed carbon electrode (SPCE) interface, and then the MB-tagged aptamer was immobilized on SPCE by using hexamethylenediamine (HMDA) as a spacer via carbodiimide amide-bonding chemistry. AFB1 has been chosen as model analyte to test the proposed aptasensing platform. A good dynamic range 2.5–30 ng L−1 was obtained for AFB1 with a limit of detection (LOD 1.6 ng L−1). The good reproducibility was recorded with RSD% of 3.21. The obtained straight-line equation was 3.6099 x + 19.073, R2 = 0.9977. For real-world sample applications, this aptasensor has been used in coffee samples. It displayed good recovery in the range 88.2–93.3%, thus showed the effectiveness of the proposed aptasensor for coffee samples. Keywords: Aflatoxin B1, Polythiophene-3-carboxylic acid (PT3C), Screen-printed carbon electrodes, Aptamer, Coffee samples
abstractGer	Aflatoxin B1 (AFB1) is the most toxic contaminant, causes carcinogenic, and immune toxic effects. There is an immense need to develop inexpensive, reliable biosensors for AFB1. The electrochemical aptasensor for detection of aflatoxin B1 has received much attention due to their trustworthy procedure. In this work, we have designed an electrochemical aptasensor by using methylene blue (MB) as a signalling fragment and polythiophene-3-carboxylic acid (PT3C) as the signal-enlarging platform. The (PT3C) was first screened onto the screen printed carbon electrode (SPCE) interface, and then the MB-tagged aptamer was immobilized on SPCE by using hexamethylenediamine (HMDA) as a spacer via carbodiimide amide-bonding chemistry. AFB1 has been chosen as model analyte to test the proposed aptasensing platform. A good dynamic range 2.5–30 ng L−1 was obtained for AFB1 with a limit of detection (LOD 1.6 ng L−1). The good reproducibility was recorded with RSD% of 3.21. The obtained straight-line equation was 3.6099 x + 19.073, R2 = 0.9977. For real-world sample applications, this aptasensor has been used in coffee samples. It displayed good recovery in the range 88.2–93.3%, thus showed the effectiveness of the proposed aptasensor for coffee samples. Keywords: Aflatoxin B1, Polythiophene-3-carboxylic acid (PT3C), Screen-printed carbon electrodes, Aptamer, Coffee samples
abstract_unstemmed	Aflatoxin B1 (AFB1) is the most toxic contaminant, causes carcinogenic, and immune toxic effects. There is an immense need to develop inexpensive, reliable biosensors for AFB1. The electrochemical aptasensor for detection of aflatoxin B1 has received much attention due to their trustworthy procedure. In this work, we have designed an electrochemical aptasensor by using methylene blue (MB) as a signalling fragment and polythiophene-3-carboxylic acid (PT3C) as the signal-enlarging platform. The (PT3C) was first screened onto the screen printed carbon electrode (SPCE) interface, and then the MB-tagged aptamer was immobilized on SPCE by using hexamethylenediamine (HMDA) as a spacer via carbodiimide amide-bonding chemistry. AFB1 has been chosen as model analyte to test the proposed aptasensing platform. A good dynamic range 2.5–30 ng L−1 was obtained for AFB1 with a limit of detection (LOD 1.6 ng L−1). The good reproducibility was recorded with RSD% of 3.21. The obtained straight-line equation was 3.6099 x + 19.073, R2 = 0.9977. For real-world sample applications, this aptasensor has been used in coffee samples. It displayed good recovery in the range 88.2–93.3%, thus showed the effectiveness of the proposed aptasensor for coffee samples. Keywords: Aflatoxin B1, Polythiophene-3-carboxylic acid (PT3C), Screen-printed carbon electrodes, Aptamer, Coffee samples
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title_short	An electrochemical aptasensor based on polythiophene-3-carboxylic acid assisted methylene blue for aflatoxin B1 detection
url	https://doi.org/10.1016/j.sbsr.2019.100290 https://doaj.org/article/4a17ad5f7f5d4670a5714548bff15d4e http://www.sciencedirect.com/science/article/pii/S2214180419300480 https://doaj.org/toc/2214-1804
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fullrecord_marcxml	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ049466801</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230308143349.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230227s2019 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1016/j.sbsr.2019.100290</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ049466801</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJ4a17ad5f7f5d4670a5714548bff15d4e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">TA1-2040</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Hanane Zejli</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="3"><subfield code="a">An electrochemical aptasensor based on polythiophene-3-carboxylic acid assisted methylene blue for aflatoxin B1 detection</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2019</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Aflatoxin B1 (AFB1) is the most toxic contaminant, causes carcinogenic, and immune toxic effects. There is an immense need to develop inexpensive, reliable biosensors for AFB1. The electrochemical aptasensor for detection of aflatoxin B1 has received much attention due to their trustworthy procedure. In this work, we have designed an electrochemical aptasensor by using methylene blue (MB) as a signalling fragment and polythiophene-3-carboxylic acid (PT3C) as the signal-enlarging platform. The (PT3C) was first screened onto the screen printed carbon electrode (SPCE) interface, and then the MB-tagged aptamer was immobilized on SPCE by using hexamethylenediamine (HMDA) as a spacer via carbodiimide amide-bonding chemistry. AFB1 has been chosen as model analyte to test the proposed aptasensing platform. A good dynamic range 2.5–30 ng L−1 was obtained for AFB1 with a limit of detection (LOD 1.6 ng L−1). The good reproducibility was recorded with RSD% of 3.21. The obtained straight-line equation was 3.6099 x + 19.073, R2 = 0.9977. For real-world sample applications, this aptasensor has been used in coffee samples. It displayed good recovery in the range 88.2–93.3%, thus showed the effectiveness of the proposed aptasensor for coffee samples. Keywords: Aflatoxin B1, Polythiophene-3-carboxylic acid (PT3C), Screen-printed carbon electrodes, Aptamer, Coffee samples</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Engineering (General). Civil engineering (General)</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">K. Yugender Goud</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Jean Louis Marty</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">Sensing and Bio-Sensing Research</subfield><subfield code="d">Elsevier, 2016</subfield><subfield code="g">25(2019), Seite -</subfield><subfield code="w">(DE-627)826105408</subfield><subfield code="w">(DE-600)2821969-7</subfield><subfield code="x">22141804</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:25</subfield><subfield code="g">year:2019</subfield><subfield code="g">pages:-</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.sbsr.2019.100290</subfield><subfield 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An electrochemical aptasensor based on polythiophene-3-carboxylic acid assisted methylene blue for aflatoxin B1 detection

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