S-phase cell cycle arrest, and apoptotic potential of Echium arabicum phenolic fraction in hepatocellular carcinoma HepG2 cells

Plants have been used in the treatment of many diseases. The aim of the present investigation was to assess the cytotoxic potential of Echium arabicum extracts against liver and colon cancer cells. The plant was extracted using Soxhlet apparats and ultrasound-assisted hydrolysis. The phenol and anti...
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Autor*in:	Nael Abutaha [verfasserIn] Fahd A. AL-Mekhlafi [verfasserIn] Bader O. Almutairi [verfasserIn] Muhammad A. Wadaan [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	Echium arabicum Apoptosis Phenols Antioxidant Liver cancer Caspase-3/7

Übergeordnetes Werk:	In: Journal of King Saud University: Science - Elsevier, 2016, 34(2022), 1, Seite 101735-
Übergeordnetes Werk:	volume:34 ; year:2022 ; number:1 ; pages:101735-

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.jksus.2021.101735

Katalog-ID:	DOAJ049530127

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520			\|a Plants have been used in the treatment of many diseases. The aim of the present investigation was to assess the cytotoxic potential of Echium arabicum extracts against liver and colon cancer cells. The plant was extracted using Soxhlet apparats and ultrasound-assisted hydrolysis. The phenol and antioxidant activity was assessed using Folin–Ciocalteu and DPPH method respectively. The cytotoxicity was determined using MTT and lactate dehydrogenase release assay. The apoptosis study was conducted using DAPI, and Acridine orange/ethidium bromide and confirmed by Caspase 3/7 Fluorescence reagent, and the Cell cycle analysis was carried out using Muse Cell Analyzer. E. arabicum had cytotoxic properties with half inhibitory concentration (IC50) values of 61.6 ± 0.6, 66.4 ± 0.9, 71.9 ± 0.8, and 38.3 ± 0.3 μg/mL against LoVo, HCT116, HuH-7 and HepG2 respectively using MTT assay. Interestingly, the phenolic fraction showed less cytotoxicity at the highest concentration tested on normal human liver (Chang) cells. Staining of HepG2 cells with DAPI and AO/EB post treatment with phenolic fraction (40 and 60 µg/mL) decreased the number of viable cells and exhibited typical features of apoptosis like blebbing of plasma membrane with an increase in the Caspase-3/7 activity. Furthermore, cell cycle study revealed that E. arabicum induced a S-phase arrest in HepG2 cells. The coefficient of determination (R2 = 0.997) and the Pearson’s correlation coefficient (r = 0.998) between DPPH activity and total phenolic content was highly correlated. These results demonstrate that phenolic fraction from E. arabicum might have a strong potential as chemopreventive agent in liver cancer.
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publishDate	2022
allfields	10.1016/j.jksus.2021.101735 doi (DE-627)DOAJ049530127 (DE-599)DOAJf2b8c68e1cb74d6db28f2f8737e37065 DE-627 ger DE-627 rakwb eng Q1-390 Nael Abutaha verfasserin aut S-phase cell cycle arrest, and apoptotic potential of Echium arabicum phenolic fraction in hepatocellular carcinoma HepG2 cells 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Plants have been used in the treatment of many diseases. The aim of the present investigation was to assess the cytotoxic potential of Echium arabicum extracts against liver and colon cancer cells. The plant was extracted using Soxhlet apparats and ultrasound-assisted hydrolysis. The phenol and antioxidant activity was assessed using Folin–Ciocalteu and DPPH method respectively. The cytotoxicity was determined using MTT and lactate dehydrogenase release assay. The apoptosis study was conducted using DAPI, and Acridine orange/ethidium bromide and confirmed by Caspase 3/7 Fluorescence reagent, and the Cell cycle analysis was carried out using Muse Cell Analyzer. E. arabicum had cytotoxic properties with half inhibitory concentration (IC50) values of 61.6 ± 0.6, 66.4 ± 0.9, 71.9 ± 0.8, and 38.3 ± 0.3 μg/mL against LoVo, HCT116, HuH-7 and HepG2 respectively using MTT assay. Interestingly, the phenolic fraction showed less cytotoxicity at the highest concentration tested on normal human liver (Chang) cells. Staining of HepG2 cells with DAPI and AO/EB post treatment with phenolic fraction (40 and 60 µg/mL) decreased the number of viable cells and exhibited typical features of apoptosis like blebbing of plasma membrane with an increase in the Caspase-3/7 activity. Furthermore, cell cycle study revealed that E. arabicum induced a S-phase arrest in HepG2 cells. The coefficient of determination (R2 = 0.997) and the Pearson’s correlation coefficient (r = 0.998) between DPPH activity and total phenolic content was highly correlated. These results demonstrate that phenolic fraction from E. arabicum might have a strong potential as chemopreventive agent in liver cancer. Echium arabicum Apoptosis Phenols Antioxidant Liver cancer Caspase-3/7 Science (General) Fahd A. AL-Mekhlafi verfasserin aut Bader O. Almutairi verfasserin aut Muhammad A. Wadaan verfasserin aut In Journal of King Saud University: Science Elsevier, 2016 34(2022), 1, Seite 101735- (DE-627)608942790 (DE-600)2514731-6 10183647 nnns volume:34 year:2022 number:1 pages:101735- https://doi.org/10.1016/j.jksus.2021.101735 kostenfrei https://doaj.org/article/f2b8c68e1cb74d6db28f2f8737e37065 kostenfrei http://www.sciencedirect.com/science/article/pii/S1018364721003979 kostenfrei https://doaj.org/toc/1018-3647 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 34 2022 1 101735-
spelling	10.1016/j.jksus.2021.101735 doi (DE-627)DOAJ049530127 (DE-599)DOAJf2b8c68e1cb74d6db28f2f8737e37065 DE-627 ger DE-627 rakwb eng Q1-390 Nael Abutaha verfasserin aut S-phase cell cycle arrest, and apoptotic potential of Echium arabicum phenolic fraction in hepatocellular carcinoma HepG2 cells 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Plants have been used in the treatment of many diseases. The aim of the present investigation was to assess the cytotoxic potential of Echium arabicum extracts against liver and colon cancer cells. The plant was extracted using Soxhlet apparats and ultrasound-assisted hydrolysis. The phenol and antioxidant activity was assessed using Folin–Ciocalteu and DPPH method respectively. The cytotoxicity was determined using MTT and lactate dehydrogenase release assay. The apoptosis study was conducted using DAPI, and Acridine orange/ethidium bromide and confirmed by Caspase 3/7 Fluorescence reagent, and the Cell cycle analysis was carried out using Muse Cell Analyzer. E. arabicum had cytotoxic properties with half inhibitory concentration (IC50) values of 61.6 ± 0.6, 66.4 ± 0.9, 71.9 ± 0.8, and 38.3 ± 0.3 μg/mL against LoVo, HCT116, HuH-7 and HepG2 respectively using MTT assay. Interestingly, the phenolic fraction showed less cytotoxicity at the highest concentration tested on normal human liver (Chang) cells. Staining of HepG2 cells with DAPI and AO/EB post treatment with phenolic fraction (40 and 60 µg/mL) decreased the number of viable cells and exhibited typical features of apoptosis like blebbing of plasma membrane with an increase in the Caspase-3/7 activity. Furthermore, cell cycle study revealed that E. arabicum induced a S-phase arrest in HepG2 cells. The coefficient of determination (R2 = 0.997) and the Pearson’s correlation coefficient (r = 0.998) between DPPH activity and total phenolic content was highly correlated. These results demonstrate that phenolic fraction from E. arabicum might have a strong potential as chemopreventive agent in liver cancer. Echium arabicum Apoptosis Phenols Antioxidant Liver cancer Caspase-3/7 Science (General) Fahd A. AL-Mekhlafi verfasserin aut Bader O. Almutairi verfasserin aut Muhammad A. Wadaan verfasserin aut In Journal of King Saud University: Science Elsevier, 2016 34(2022), 1, Seite 101735- (DE-627)608942790 (DE-600)2514731-6 10183647 nnns volume:34 year:2022 number:1 pages:101735- https://doi.org/10.1016/j.jksus.2021.101735 kostenfrei https://doaj.org/article/f2b8c68e1cb74d6db28f2f8737e37065 kostenfrei http://www.sciencedirect.com/science/article/pii/S1018364721003979 kostenfrei https://doaj.org/toc/1018-3647 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 34 2022 1 101735-
allfields_unstemmed	10.1016/j.jksus.2021.101735 doi (DE-627)DOAJ049530127 (DE-599)DOAJf2b8c68e1cb74d6db28f2f8737e37065 DE-627 ger DE-627 rakwb eng Q1-390 Nael Abutaha verfasserin aut S-phase cell cycle arrest, and apoptotic potential of Echium arabicum phenolic fraction in hepatocellular carcinoma HepG2 cells 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Plants have been used in the treatment of many diseases. The aim of the present investigation was to assess the cytotoxic potential of Echium arabicum extracts against liver and colon cancer cells. The plant was extracted using Soxhlet apparats and ultrasound-assisted hydrolysis. The phenol and antioxidant activity was assessed using Folin–Ciocalteu and DPPH method respectively. The cytotoxicity was determined using MTT and lactate dehydrogenase release assay. The apoptosis study was conducted using DAPI, and Acridine orange/ethidium bromide and confirmed by Caspase 3/7 Fluorescence reagent, and the Cell cycle analysis was carried out using Muse Cell Analyzer. E. arabicum had cytotoxic properties with half inhibitory concentration (IC50) values of 61.6 ± 0.6, 66.4 ± 0.9, 71.9 ± 0.8, and 38.3 ± 0.3 μg/mL against LoVo, HCT116, HuH-7 and HepG2 respectively using MTT assay. Interestingly, the phenolic fraction showed less cytotoxicity at the highest concentration tested on normal human liver (Chang) cells. Staining of HepG2 cells with DAPI and AO/EB post treatment with phenolic fraction (40 and 60 µg/mL) decreased the number of viable cells and exhibited typical features of apoptosis like blebbing of plasma membrane with an increase in the Caspase-3/7 activity. Furthermore, cell cycle study revealed that E. arabicum induced a S-phase arrest in HepG2 cells. The coefficient of determination (R2 = 0.997) and the Pearson’s correlation coefficient (r = 0.998) between DPPH activity and total phenolic content was highly correlated. These results demonstrate that phenolic fraction from E. arabicum might have a strong potential as chemopreventive agent in liver cancer. Echium arabicum Apoptosis Phenols Antioxidant Liver cancer Caspase-3/7 Science (General) Fahd A. AL-Mekhlafi verfasserin aut Bader O. Almutairi verfasserin aut Muhammad A. Wadaan verfasserin aut In Journal of King Saud University: Science Elsevier, 2016 34(2022), 1, Seite 101735- (DE-627)608942790 (DE-600)2514731-6 10183647 nnns volume:34 year:2022 number:1 pages:101735- https://doi.org/10.1016/j.jksus.2021.101735 kostenfrei https://doaj.org/article/f2b8c68e1cb74d6db28f2f8737e37065 kostenfrei http://www.sciencedirect.com/science/article/pii/S1018364721003979 kostenfrei https://doaj.org/toc/1018-3647 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 34 2022 1 101735-
allfieldsGer	10.1016/j.jksus.2021.101735 doi (DE-627)DOAJ049530127 (DE-599)DOAJf2b8c68e1cb74d6db28f2f8737e37065 DE-627 ger DE-627 rakwb eng Q1-390 Nael Abutaha verfasserin aut S-phase cell cycle arrest, and apoptotic potential of Echium arabicum phenolic fraction in hepatocellular carcinoma HepG2 cells 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Plants have been used in the treatment of many diseases. The aim of the present investigation was to assess the cytotoxic potential of Echium arabicum extracts against liver and colon cancer cells. The plant was extracted using Soxhlet apparats and ultrasound-assisted hydrolysis. The phenol and antioxidant activity was assessed using Folin–Ciocalteu and DPPH method respectively. The cytotoxicity was determined using MTT and lactate dehydrogenase release assay. The apoptosis study was conducted using DAPI, and Acridine orange/ethidium bromide and confirmed by Caspase 3/7 Fluorescence reagent, and the Cell cycle analysis was carried out using Muse Cell Analyzer. E. arabicum had cytotoxic properties with half inhibitory concentration (IC50) values of 61.6 ± 0.6, 66.4 ± 0.9, 71.9 ± 0.8, and 38.3 ± 0.3 μg/mL against LoVo, HCT116, HuH-7 and HepG2 respectively using MTT assay. Interestingly, the phenolic fraction showed less cytotoxicity at the highest concentration tested on normal human liver (Chang) cells. Staining of HepG2 cells with DAPI and AO/EB post treatment with phenolic fraction (40 and 60 µg/mL) decreased the number of viable cells and exhibited typical features of apoptosis like blebbing of plasma membrane with an increase in the Caspase-3/7 activity. Furthermore, cell cycle study revealed that E. arabicum induced a S-phase arrest in HepG2 cells. The coefficient of determination (R2 = 0.997) and the Pearson’s correlation coefficient (r = 0.998) between DPPH activity and total phenolic content was highly correlated. These results demonstrate that phenolic fraction from E. arabicum might have a strong potential as chemopreventive agent in liver cancer. Echium arabicum Apoptosis Phenols Antioxidant Liver cancer Caspase-3/7 Science (General) Fahd A. AL-Mekhlafi verfasserin aut Bader O. Almutairi verfasserin aut Muhammad A. Wadaan verfasserin aut In Journal of King Saud University: Science Elsevier, 2016 34(2022), 1, Seite 101735- (DE-627)608942790 (DE-600)2514731-6 10183647 nnns volume:34 year:2022 number:1 pages:101735- https://doi.org/10.1016/j.jksus.2021.101735 kostenfrei https://doaj.org/article/f2b8c68e1cb74d6db28f2f8737e37065 kostenfrei http://www.sciencedirect.com/science/article/pii/S1018364721003979 kostenfrei https://doaj.org/toc/1018-3647 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 34 2022 1 101735-
allfieldsSound	10.1016/j.jksus.2021.101735 doi (DE-627)DOAJ049530127 (DE-599)DOAJf2b8c68e1cb74d6db28f2f8737e37065 DE-627 ger DE-627 rakwb eng Q1-390 Nael Abutaha verfasserin aut S-phase cell cycle arrest, and apoptotic potential of Echium arabicum phenolic fraction in hepatocellular carcinoma HepG2 cells 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Plants have been used in the treatment of many diseases. The aim of the present investigation was to assess the cytotoxic potential of Echium arabicum extracts against liver and colon cancer cells. The plant was extracted using Soxhlet apparats and ultrasound-assisted hydrolysis. The phenol and antioxidant activity was assessed using Folin–Ciocalteu and DPPH method respectively. The cytotoxicity was determined using MTT and lactate dehydrogenase release assay. The apoptosis study was conducted using DAPI, and Acridine orange/ethidium bromide and confirmed by Caspase 3/7 Fluorescence reagent, and the Cell cycle analysis was carried out using Muse Cell Analyzer. E. arabicum had cytotoxic properties with half inhibitory concentration (IC50) values of 61.6 ± 0.6, 66.4 ± 0.9, 71.9 ± 0.8, and 38.3 ± 0.3 μg/mL against LoVo, HCT116, HuH-7 and HepG2 respectively using MTT assay. Interestingly, the phenolic fraction showed less cytotoxicity at the highest concentration tested on normal human liver (Chang) cells. Staining of HepG2 cells with DAPI and AO/EB post treatment with phenolic fraction (40 and 60 µg/mL) decreased the number of viable cells and exhibited typical features of apoptosis like blebbing of plasma membrane with an increase in the Caspase-3/7 activity. Furthermore, cell cycle study revealed that E. arabicum induced a S-phase arrest in HepG2 cells. The coefficient of determination (R2 = 0.997) and the Pearson’s correlation coefficient (r = 0.998) between DPPH activity and total phenolic content was highly correlated. These results demonstrate that phenolic fraction from E. arabicum might have a strong potential as chemopreventive agent in liver cancer. Echium arabicum Apoptosis Phenols Antioxidant Liver cancer Caspase-3/7 Science (General) Fahd A. AL-Mekhlafi verfasserin aut Bader O. Almutairi verfasserin aut Muhammad A. Wadaan verfasserin aut In Journal of King Saud University: Science Elsevier, 2016 34(2022), 1, Seite 101735- (DE-627)608942790 (DE-600)2514731-6 10183647 nnns volume:34 year:2022 number:1 pages:101735- https://doi.org/10.1016/j.jksus.2021.101735 kostenfrei https://doaj.org/article/f2b8c68e1cb74d6db28f2f8737e37065 kostenfrei http://www.sciencedirect.com/science/article/pii/S1018364721003979 kostenfrei https://doaj.org/toc/1018-3647 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 34 2022 1 101735-
language	English
source	In Journal of King Saud University: Science 34(2022), 1, Seite 101735- volume:34 year:2022 number:1 pages:101735-
sourceStr	In Journal of King Saud University: Science 34(2022), 1, Seite 101735- volume:34 year:2022 number:1 pages:101735-
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topic_facet	Echium arabicum Apoptosis Phenols Antioxidant Liver cancer Caspase-3/7 Science (General)
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container_title	Journal of King Saud University: Science
authorswithroles_txt_mv	Nael Abutaha @@aut@@ Fahd A. AL-Mekhlafi @@aut@@ Bader O. Almutairi @@aut@@ Muhammad A. Wadaan @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
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callnumber-first	Q - Science
author	Nael Abutaha
spellingShingle	Nael Abutaha misc Q1-390 misc Echium arabicum misc Apoptosis misc Phenols misc Antioxidant misc Liver cancer misc Caspase-3/7 misc Science (General) S-phase cell cycle arrest, and apoptotic potential of Echium arabicum phenolic fraction in hepatocellular carcinoma HepG2 cells
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topic_title	Q1-390 S-phase cell cycle arrest, and apoptotic potential of Echium arabicum phenolic fraction in hepatocellular carcinoma HepG2 cells Echium arabicum Apoptosis Phenols Antioxidant Liver cancer Caspase-3/7
topic	misc Q1-390 misc Echium arabicum misc Apoptosis misc Phenols misc Antioxidant misc Liver cancer misc Caspase-3/7 misc Science (General)
topic_unstemmed	misc Q1-390 misc Echium arabicum misc Apoptosis misc Phenols misc Antioxidant misc Liver cancer misc Caspase-3/7 misc Science (General)
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title	S-phase cell cycle arrest, and apoptotic potential of Echium arabicum phenolic fraction in hepatocellular carcinoma HepG2 cells
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title_full	S-phase cell cycle arrest, and apoptotic potential of Echium arabicum phenolic fraction in hepatocellular carcinoma HepG2 cells
author_sort	Nael Abutaha
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author_browse	Nael Abutaha Fahd A. AL-Mekhlafi Bader O. Almutairi Muhammad A. Wadaan
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title_sort	s-phase cell cycle arrest, and apoptotic potential of echium arabicum phenolic fraction in hepatocellular carcinoma hepg2 cells
callnumber	Q1-390
title_auth	S-phase cell cycle arrest, and apoptotic potential of Echium arabicum phenolic fraction in hepatocellular carcinoma HepG2 cells
abstract	Plants have been used in the treatment of many diseases. The aim of the present investigation was to assess the cytotoxic potential of Echium arabicum extracts against liver and colon cancer cells. The plant was extracted using Soxhlet apparats and ultrasound-assisted hydrolysis. The phenol and antioxidant activity was assessed using Folin–Ciocalteu and DPPH method respectively. The cytotoxicity was determined using MTT and lactate dehydrogenase release assay. The apoptosis study was conducted using DAPI, and Acridine orange/ethidium bromide and confirmed by Caspase 3/7 Fluorescence reagent, and the Cell cycle analysis was carried out using Muse Cell Analyzer. E. arabicum had cytotoxic properties with half inhibitory concentration (IC50) values of 61.6 ± 0.6, 66.4 ± 0.9, 71.9 ± 0.8, and 38.3 ± 0.3 μg/mL against LoVo, HCT116, HuH-7 and HepG2 respectively using MTT assay. Interestingly, the phenolic fraction showed less cytotoxicity at the highest concentration tested on normal human liver (Chang) cells. Staining of HepG2 cells with DAPI and AO/EB post treatment with phenolic fraction (40 and 60 µg/mL) decreased the number of viable cells and exhibited typical features of apoptosis like blebbing of plasma membrane with an increase in the Caspase-3/7 activity. Furthermore, cell cycle study revealed that E. arabicum induced a S-phase arrest in HepG2 cells. The coefficient of determination (R2 = 0.997) and the Pearson’s correlation coefficient (r = 0.998) between DPPH activity and total phenolic content was highly correlated. These results demonstrate that phenolic fraction from E. arabicum might have a strong potential as chemopreventive agent in liver cancer.
abstractGer	Plants have been used in the treatment of many diseases. The aim of the present investigation was to assess the cytotoxic potential of Echium arabicum extracts against liver and colon cancer cells. The plant was extracted using Soxhlet apparats and ultrasound-assisted hydrolysis. The phenol and antioxidant activity was assessed using Folin–Ciocalteu and DPPH method respectively. The cytotoxicity was determined using MTT and lactate dehydrogenase release assay. The apoptosis study was conducted using DAPI, and Acridine orange/ethidium bromide and confirmed by Caspase 3/7 Fluorescence reagent, and the Cell cycle analysis was carried out using Muse Cell Analyzer. E. arabicum had cytotoxic properties with half inhibitory concentration (IC50) values of 61.6 ± 0.6, 66.4 ± 0.9, 71.9 ± 0.8, and 38.3 ± 0.3 μg/mL against LoVo, HCT116, HuH-7 and HepG2 respectively using MTT assay. Interestingly, the phenolic fraction showed less cytotoxicity at the highest concentration tested on normal human liver (Chang) cells. Staining of HepG2 cells with DAPI and AO/EB post treatment with phenolic fraction (40 and 60 µg/mL) decreased the number of viable cells and exhibited typical features of apoptosis like blebbing of plasma membrane with an increase in the Caspase-3/7 activity. Furthermore, cell cycle study revealed that E. arabicum induced a S-phase arrest in HepG2 cells. The coefficient of determination (R2 = 0.997) and the Pearson’s correlation coefficient (r = 0.998) between DPPH activity and total phenolic content was highly correlated. These results demonstrate that phenolic fraction from E. arabicum might have a strong potential as chemopreventive agent in liver cancer.
abstract_unstemmed	Plants have been used in the treatment of many diseases. The aim of the present investigation was to assess the cytotoxic potential of Echium arabicum extracts against liver and colon cancer cells. The plant was extracted using Soxhlet apparats and ultrasound-assisted hydrolysis. The phenol and antioxidant activity was assessed using Folin–Ciocalteu and DPPH method respectively. The cytotoxicity was determined using MTT and lactate dehydrogenase release assay. The apoptosis study was conducted using DAPI, and Acridine orange/ethidium bromide and confirmed by Caspase 3/7 Fluorescence reagent, and the Cell cycle analysis was carried out using Muse Cell Analyzer. E. arabicum had cytotoxic properties with half inhibitory concentration (IC50) values of 61.6 ± 0.6, 66.4 ± 0.9, 71.9 ± 0.8, and 38.3 ± 0.3 μg/mL against LoVo, HCT116, HuH-7 and HepG2 respectively using MTT assay. Interestingly, the phenolic fraction showed less cytotoxicity at the highest concentration tested on normal human liver (Chang) cells. Staining of HepG2 cells with DAPI and AO/EB post treatment with phenolic fraction (40 and 60 µg/mL) decreased the number of viable cells and exhibited typical features of apoptosis like blebbing of plasma membrane with an increase in the Caspase-3/7 activity. Furthermore, cell cycle study revealed that E. arabicum induced a S-phase arrest in HepG2 cells. The coefficient of determination (R2 = 0.997) and the Pearson’s correlation coefficient (r = 0.998) between DPPH activity and total phenolic content was highly correlated. These results demonstrate that phenolic fraction from E. arabicum might have a strong potential as chemopreventive agent in liver cancer.
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title_short	S-phase cell cycle arrest, and apoptotic potential of Echium arabicum phenolic fraction in hepatocellular carcinoma HepG2 cells
url	https://doi.org/10.1016/j.jksus.2021.101735 https://doaj.org/article/f2b8c68e1cb74d6db28f2f8737e37065 http://www.sciencedirect.com/science/article/pii/S1018364721003979 https://doaj.org/toc/1018-3647
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The aim of the present investigation was to assess the cytotoxic potential of Echium arabicum extracts against liver and colon cancer cells. The plant was extracted using Soxhlet apparats and ultrasound-assisted hydrolysis. The phenol and antioxidant activity was assessed using Folin–Ciocalteu and DPPH method respectively. The cytotoxicity was determined using MTT and lactate dehydrogenase release assay. The apoptosis study was conducted using DAPI, and Acridine orange/ethidium bromide and confirmed by Caspase 3/7 Fluorescence reagent, and the Cell cycle analysis was carried out using Muse Cell Analyzer. E. arabicum had cytotoxic properties with half inhibitory concentration (IC50) values of 61.6 ± 0.6, 66.4 ± 0.9, 71.9 ± 0.8, and 38.3 ± 0.3 μg/mL against LoVo, HCT116, HuH-7 and HepG2 respectively using MTT assay. Interestingly, the phenolic fraction showed less cytotoxicity at the highest concentration tested on normal human liver (Chang) cells. Staining of HepG2 cells with DAPI and AO/EB post treatment with phenolic fraction (40 and 60 µg/mL) decreased the number of viable cells and exhibited typical features of apoptosis like blebbing of plasma membrane with an increase in the Caspase-3/7 activity. Furthermore, cell cycle study revealed that E. arabicum induced a S-phase arrest in HepG2 cells. The coefficient of determination (R2 = 0.997) and the Pearson’s correlation coefficient (r = 0.998) between DPPH activity and total phenolic content was highly correlated. 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S-phase cell cycle arrest, and apoptotic potential of Echium arabicum phenolic fraction in hepatocellular carcinoma HepG2 cells

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