Association between CTLA-4 gene polymorphism and the risk of systemic lupus erythematosus: brief report

Background: Cytotoxic lymphocyte antigen-4 (CTLA-4) plays an important role in regulating T cell activation. CTLA-4 gene polymorphisms are related with genetic susceptibility to various autoimmune diseases, including systemic lupus erythematosus (SLE). We analyzed the role of CTLA-4 polymorphisms at positions -318CT in patients who suffer from SLE. Methods: This study was performed on 180 SLE patients referred to 5th Azar University Hospital in Gorgan, Iran. Three hundred and four ethnically-and age-matched healthy controls with no history of autoimmune diseases entered the study between 5th May 2008 and 23rd October 2009. DNA was extracted from blood samples according to the standard procedure. Polymerase chain reaction- restriction fragments length polymorphism (PCR-RFLP) was used to analyze the genotype and allele frequencies of this polymorphism. PCR was carried out using the following primers: forward 5′-AAATGAATTGGACTGGATGGT-3′ and reverse 5′-TTACGAGAAAGGAAGCCGT G-3′. The frequency of alleles and genotypes were assessed using direct counting. Chi-square test and Fisher’s exact test were used to compare the association between the alleles and genotype frequencies and SLE. P<0.05 were considered statistically significant. Results: The CC genotype was observed in 94.5% of the SLE patients and 82.4% of the controls; the difference was statistically significant (P=0.0001, OR=3.51, CI95%=1.77-7.53). The CT genotype, on the other hand, was more frequently observed in the control group (17.1% vs. 5.5%, P=0.0001, OR=0.28). T allele was significantly more common in the controls compared to SLE patients (P=0.0001, OR=0.26, CI95%=0.13-0.53). Conclusion: Our results suggest that the -318C/T polymorphism of CTLA-4 gene might play a significant role in the genetic susceptibility to SLE. Therefore, further studies on populations, especially from other Middle East countries, are needed to confirm our results. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Mahdieh Shojaa [verfasserIn] Mehrdad Aghaie [verfasserIn] Mahsa Amoli [verfasserIn] Patricia Khashayar [verfasserIn] Naemeh Javid [verfasserIn] Fatemeh Shakeri [verfasserIn] Mostafa Qorbani [verfasserIn] Ramin Mohebbi [verfasserIn]

Format:	E-Artikel
Sprache:	Persisch

Erschienen:	2015

Schlagwörter:	CTLA-4 polymorphism genetics promoter regions systemic lupus erythematosus.

Übergeordnetes Werk:	In: Tehran University Medical Journal - Tehran University of Medical Sciences, 2007, 73(2015), 2, Seite 127-131
Übergeordnetes Werk:	volume:73 ; year:2015 ; number:2 ; pages:127-131

Links:	Link aufrufen Link aufrufen Journal toc Journal toc

Katalog-ID:	DOAJ050063375

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520			\|a Background: Cytotoxic lymphocyte antigen-4 (CTLA-4) plays an important role in regulating T cell activation. CTLA-4 gene polymorphisms are related with genetic susceptibility to various autoimmune diseases, including systemic lupus erythematosus (SLE). We analyzed the role of CTLA-4 polymorphisms at positions -318CT in patients who suffer from SLE. Methods: This study was performed on 180 SLE patients referred to 5th Azar University Hospital in Gorgan, Iran. Three hundred and four ethnically-and age-matched healthy controls with no history of autoimmune diseases entered the study between 5th May 2008 and 23rd October 2009. DNA was extracted from blood samples according to the standard procedure. Polymerase chain reaction- restriction fragments length polymorphism (PCR-RFLP) was used to analyze the genotype and allele frequencies of this polymorphism. PCR was carried out using the following primers: forward 5′-AAATGAATTGGACTGGATGGT-3′ and reverse 5′-TTACGAGAAAGGAAGCCGT G-3′. The frequency of alleles and genotypes were assessed using direct counting. Chi-square test and Fisher’s exact test were used to compare the association between the alleles and genotype frequencies and SLE. P<0.05 were considered statistically significant. Results: The CC genotype was observed in 94.5% of the SLE patients and 82.4% of the controls; the difference was statistically significant (P=0.0001, OR=3.51, CI95%=1.77-7.53). The CT genotype, on the other hand, was more frequently observed in the control group (17.1% vs. 5.5%, P=0.0001, OR=0.28). T allele was significantly more common in the controls compared to SLE patients (P=0.0001, OR=0.26, CI95%=0.13-0.53). Conclusion: Our results suggest that the -318C/T polymorphism of CTLA-4 gene might play a significant role in the genetic susceptibility to SLE. Therefore, further studies on populations, especially from other Middle East countries, are needed to confirm our results.
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allfields	(DE-627)DOAJ050063375 (DE-599)DOAJc393d091fa3f4f2bbf750c01fd34d201 DE-627 ger DE-627 rakwb per R5-920 Mahdieh Shojaa verfasserin aut Association between CTLA-4 gene polymorphism and the risk of systemic lupus erythematosus: brief report 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Cytotoxic lymphocyte antigen-4 (CTLA-4) plays an important role in regulating T cell activation. CTLA-4 gene polymorphisms are related with genetic susceptibility to various autoimmune diseases, including systemic lupus erythematosus (SLE). We analyzed the role of CTLA-4 polymorphisms at positions -318CT in patients who suffer from SLE. Methods: This study was performed on 180 SLE patients referred to 5th Azar University Hospital in Gorgan, Iran. Three hundred and four ethnically-and age-matched healthy controls with no history of autoimmune diseases entered the study between 5th May 2008 and 23rd October 2009. DNA was extracted from blood samples according to the standard procedure. Polymerase chain reaction- restriction fragments length polymorphism (PCR-RFLP) was used to analyze the genotype and allele frequencies of this polymorphism. PCR was carried out using the following primers: forward 5′-AAATGAATTGGACTGGATGGT-3′ and reverse 5′-TTACGAGAAAGGAAGCCGT G-3′. The frequency of alleles and genotypes were assessed using direct counting. Chi-square test and Fisher’s exact test were used to compare the association between the alleles and genotype frequencies and SLE. P<0.05 were considered statistically significant. Results: The CC genotype was observed in 94.5% of the SLE patients and 82.4% of the controls; the difference was statistically significant (P=0.0001, OR=3.51, CI95%=1.77-7.53). The CT genotype, on the other hand, was more frequently observed in the control group (17.1% vs. 5.5%, P=0.0001, OR=0.28). T allele was significantly more common in the controls compared to SLE patients (P=0.0001, OR=0.26, CI95%=0.13-0.53). Conclusion: Our results suggest that the -318C/T polymorphism of CTLA-4 gene might play a significant role in the genetic susceptibility to SLE. Therefore, further studies on populations, especially from other Middle East countries, are needed to confirm our results. CTLA-4 polymorphism genetics promoter regions systemic lupus erythematosus. Medicine (General) Mehrdad Aghaie verfasserin aut Mahsa Amoli verfasserin aut Patricia Khashayar verfasserin aut Naemeh Javid verfasserin aut Fatemeh Shakeri verfasserin aut Mostafa Qorbani verfasserin aut Ramin Mohebbi verfasserin aut In Tehran University Medical Journal Tehran University of Medical Sciences, 2007 73(2015), 2, Seite 127-131 (DE-627)590956442 (DE-600)2477016-4 17357322 nnns volume:73 year:2015 number:2 pages:127-131 https://doaj.org/article/c393d091fa3f4f2bbf750c01fd34d201 kostenfrei http://tumj.tums.ac.ir/browse.php?a_code=A-10-25-5326&slc_lang=en&sid=1 kostenfrei https://doaj.org/toc/1683-1764 Journal toc kostenfrei https://doaj.org/toc/1735-7322 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 73 2015 2 127-131
spelling	(DE-627)DOAJ050063375 (DE-599)DOAJc393d091fa3f4f2bbf750c01fd34d201 DE-627 ger DE-627 rakwb per R5-920 Mahdieh Shojaa verfasserin aut Association between CTLA-4 gene polymorphism and the risk of systemic lupus erythematosus: brief report 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Cytotoxic lymphocyte antigen-4 (CTLA-4) plays an important role in regulating T cell activation. CTLA-4 gene polymorphisms are related with genetic susceptibility to various autoimmune diseases, including systemic lupus erythematosus (SLE). We analyzed the role of CTLA-4 polymorphisms at positions -318CT in patients who suffer from SLE. Methods: This study was performed on 180 SLE patients referred to 5th Azar University Hospital in Gorgan, Iran. Three hundred and four ethnically-and age-matched healthy controls with no history of autoimmune diseases entered the study between 5th May 2008 and 23rd October 2009. DNA was extracted from blood samples according to the standard procedure. Polymerase chain reaction- restriction fragments length polymorphism (PCR-RFLP) was used to analyze the genotype and allele frequencies of this polymorphism. PCR was carried out using the following primers: forward 5′-AAATGAATTGGACTGGATGGT-3′ and reverse 5′-TTACGAGAAAGGAAGCCGT G-3′. The frequency of alleles and genotypes were assessed using direct counting. Chi-square test and Fisher’s exact test were used to compare the association between the alleles and genotype frequencies and SLE. P<0.05 were considered statistically significant. Results: The CC genotype was observed in 94.5% of the SLE patients and 82.4% of the controls; the difference was statistically significant (P=0.0001, OR=3.51, CI95%=1.77-7.53). The CT genotype, on the other hand, was more frequently observed in the control group (17.1% vs. 5.5%, P=0.0001, OR=0.28). T allele was significantly more common in the controls compared to SLE patients (P=0.0001, OR=0.26, CI95%=0.13-0.53). Conclusion: Our results suggest that the -318C/T polymorphism of CTLA-4 gene might play a significant role in the genetic susceptibility to SLE. Therefore, further studies on populations, especially from other Middle East countries, are needed to confirm our results. CTLA-4 polymorphism genetics promoter regions systemic lupus erythematosus. Medicine (General) Mehrdad Aghaie verfasserin aut Mahsa Amoli verfasserin aut Patricia Khashayar verfasserin aut Naemeh Javid verfasserin aut Fatemeh Shakeri verfasserin aut Mostafa Qorbani verfasserin aut Ramin Mohebbi verfasserin aut In Tehran University Medical Journal Tehran University of Medical Sciences, 2007 73(2015), 2, Seite 127-131 (DE-627)590956442 (DE-600)2477016-4 17357322 nnns volume:73 year:2015 number:2 pages:127-131 https://doaj.org/article/c393d091fa3f4f2bbf750c01fd34d201 kostenfrei http://tumj.tums.ac.ir/browse.php?a_code=A-10-25-5326&slc_lang=en&sid=1 kostenfrei https://doaj.org/toc/1683-1764 Journal toc kostenfrei https://doaj.org/toc/1735-7322 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 73 2015 2 127-131
allfields_unstemmed	(DE-627)DOAJ050063375 (DE-599)DOAJc393d091fa3f4f2bbf750c01fd34d201 DE-627 ger DE-627 rakwb per R5-920 Mahdieh Shojaa verfasserin aut Association between CTLA-4 gene polymorphism and the risk of systemic lupus erythematosus: brief report 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Cytotoxic lymphocyte antigen-4 (CTLA-4) plays an important role in regulating T cell activation. CTLA-4 gene polymorphisms are related with genetic susceptibility to various autoimmune diseases, including systemic lupus erythematosus (SLE). We analyzed the role of CTLA-4 polymorphisms at positions -318CT in patients who suffer from SLE. Methods: This study was performed on 180 SLE patients referred to 5th Azar University Hospital in Gorgan, Iran. Three hundred and four ethnically-and age-matched healthy controls with no history of autoimmune diseases entered the study between 5th May 2008 and 23rd October 2009. DNA was extracted from blood samples according to the standard procedure. Polymerase chain reaction- restriction fragments length polymorphism (PCR-RFLP) was used to analyze the genotype and allele frequencies of this polymorphism. PCR was carried out using the following primers: forward 5′-AAATGAATTGGACTGGATGGT-3′ and reverse 5′-TTACGAGAAAGGAAGCCGT G-3′. The frequency of alleles and genotypes were assessed using direct counting. Chi-square test and Fisher’s exact test were used to compare the association between the alleles and genotype frequencies and SLE. P<0.05 were considered statistically significant. Results: The CC genotype was observed in 94.5% of the SLE patients and 82.4% of the controls; the difference was statistically significant (P=0.0001, OR=3.51, CI95%=1.77-7.53). The CT genotype, on the other hand, was more frequently observed in the control group (17.1% vs. 5.5%, P=0.0001, OR=0.28). T allele was significantly more common in the controls compared to SLE patients (P=0.0001, OR=0.26, CI95%=0.13-0.53). Conclusion: Our results suggest that the -318C/T polymorphism of CTLA-4 gene might play a significant role in the genetic susceptibility to SLE. Therefore, further studies on populations, especially from other Middle East countries, are needed to confirm our results. CTLA-4 polymorphism genetics promoter regions systemic lupus erythematosus. Medicine (General) Mehrdad Aghaie verfasserin aut Mahsa Amoli verfasserin aut Patricia Khashayar verfasserin aut Naemeh Javid verfasserin aut Fatemeh Shakeri verfasserin aut Mostafa Qorbani verfasserin aut Ramin Mohebbi verfasserin aut In Tehran University Medical Journal Tehran University of Medical Sciences, 2007 73(2015), 2, Seite 127-131 (DE-627)590956442 (DE-600)2477016-4 17357322 nnns volume:73 year:2015 number:2 pages:127-131 https://doaj.org/article/c393d091fa3f4f2bbf750c01fd34d201 kostenfrei http://tumj.tums.ac.ir/browse.php?a_code=A-10-25-5326&slc_lang=en&sid=1 kostenfrei https://doaj.org/toc/1683-1764 Journal toc kostenfrei https://doaj.org/toc/1735-7322 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 73 2015 2 127-131
allfieldsGer	(DE-627)DOAJ050063375 (DE-599)DOAJc393d091fa3f4f2bbf750c01fd34d201 DE-627 ger DE-627 rakwb per R5-920 Mahdieh Shojaa verfasserin aut Association between CTLA-4 gene polymorphism and the risk of systemic lupus erythematosus: brief report 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Cytotoxic lymphocyte antigen-4 (CTLA-4) plays an important role in regulating T cell activation. CTLA-4 gene polymorphisms are related with genetic susceptibility to various autoimmune diseases, including systemic lupus erythematosus (SLE). We analyzed the role of CTLA-4 polymorphisms at positions -318CT in patients who suffer from SLE. Methods: This study was performed on 180 SLE patients referred to 5th Azar University Hospital in Gorgan, Iran. Three hundred and four ethnically-and age-matched healthy controls with no history of autoimmune diseases entered the study between 5th May 2008 and 23rd October 2009. DNA was extracted from blood samples according to the standard procedure. Polymerase chain reaction- restriction fragments length polymorphism (PCR-RFLP) was used to analyze the genotype and allele frequencies of this polymorphism. PCR was carried out using the following primers: forward 5′-AAATGAATTGGACTGGATGGT-3′ and reverse 5′-TTACGAGAAAGGAAGCCGT G-3′. The frequency of alleles and genotypes were assessed using direct counting. Chi-square test and Fisher’s exact test were used to compare the association between the alleles and genotype frequencies and SLE. P<0.05 were considered statistically significant. Results: The CC genotype was observed in 94.5% of the SLE patients and 82.4% of the controls; the difference was statistically significant (P=0.0001, OR=3.51, CI95%=1.77-7.53). The CT genotype, on the other hand, was more frequently observed in the control group (17.1% vs. 5.5%, P=0.0001, OR=0.28). T allele was significantly more common in the controls compared to SLE patients (P=0.0001, OR=0.26, CI95%=0.13-0.53). Conclusion: Our results suggest that the -318C/T polymorphism of CTLA-4 gene might play a significant role in the genetic susceptibility to SLE. Therefore, further studies on populations, especially from other Middle East countries, are needed to confirm our results. CTLA-4 polymorphism genetics promoter regions systemic lupus erythematosus. Medicine (General) Mehrdad Aghaie verfasserin aut Mahsa Amoli verfasserin aut Patricia Khashayar verfasserin aut Naemeh Javid verfasserin aut Fatemeh Shakeri verfasserin aut Mostafa Qorbani verfasserin aut Ramin Mohebbi verfasserin aut In Tehran University Medical Journal Tehran University of Medical Sciences, 2007 73(2015), 2, Seite 127-131 (DE-627)590956442 (DE-600)2477016-4 17357322 nnns volume:73 year:2015 number:2 pages:127-131 https://doaj.org/article/c393d091fa3f4f2bbf750c01fd34d201 kostenfrei http://tumj.tums.ac.ir/browse.php?a_code=A-10-25-5326&slc_lang=en&sid=1 kostenfrei https://doaj.org/toc/1683-1764 Journal toc kostenfrei https://doaj.org/toc/1735-7322 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 73 2015 2 127-131
allfieldsSound	(DE-627)DOAJ050063375 (DE-599)DOAJc393d091fa3f4f2bbf750c01fd34d201 DE-627 ger DE-627 rakwb per R5-920 Mahdieh Shojaa verfasserin aut Association between CTLA-4 gene polymorphism and the risk of systemic lupus erythematosus: brief report 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: Cytotoxic lymphocyte antigen-4 (CTLA-4) plays an important role in regulating T cell activation. CTLA-4 gene polymorphisms are related with genetic susceptibility to various autoimmune diseases, including systemic lupus erythematosus (SLE). We analyzed the role of CTLA-4 polymorphisms at positions -318CT in patients who suffer from SLE. Methods: This study was performed on 180 SLE patients referred to 5th Azar University Hospital in Gorgan, Iran. Three hundred and four ethnically-and age-matched healthy controls with no history of autoimmune diseases entered the study between 5th May 2008 and 23rd October 2009. DNA was extracted from blood samples according to the standard procedure. Polymerase chain reaction- restriction fragments length polymorphism (PCR-RFLP) was used to analyze the genotype and allele frequencies of this polymorphism. PCR was carried out using the following primers: forward 5′-AAATGAATTGGACTGGATGGT-3′ and reverse 5′-TTACGAGAAAGGAAGCCGT G-3′. The frequency of alleles and genotypes were assessed using direct counting. Chi-square test and Fisher’s exact test were used to compare the association between the alleles and genotype frequencies and SLE. P<0.05 were considered statistically significant. Results: The CC genotype was observed in 94.5% of the SLE patients and 82.4% of the controls; the difference was statistically significant (P=0.0001, OR=3.51, CI95%=1.77-7.53). The CT genotype, on the other hand, was more frequently observed in the control group (17.1% vs. 5.5%, P=0.0001, OR=0.28). T allele was significantly more common in the controls compared to SLE patients (P=0.0001, OR=0.26, CI95%=0.13-0.53). Conclusion: Our results suggest that the -318C/T polymorphism of CTLA-4 gene might play a significant role in the genetic susceptibility to SLE. Therefore, further studies on populations, especially from other Middle East countries, are needed to confirm our results. CTLA-4 polymorphism genetics promoter regions systemic lupus erythematosus. Medicine (General) Mehrdad Aghaie verfasserin aut Mahsa Amoli verfasserin aut Patricia Khashayar verfasserin aut Naemeh Javid verfasserin aut Fatemeh Shakeri verfasserin aut Mostafa Qorbani verfasserin aut Ramin Mohebbi verfasserin aut In Tehran University Medical Journal Tehran University of Medical Sciences, 2007 73(2015), 2, Seite 127-131 (DE-627)590956442 (DE-600)2477016-4 17357322 nnns volume:73 year:2015 number:2 pages:127-131 https://doaj.org/article/c393d091fa3f4f2bbf750c01fd34d201 kostenfrei http://tumj.tums.ac.ir/browse.php?a_code=A-10-25-5326&slc_lang=en&sid=1 kostenfrei https://doaj.org/toc/1683-1764 Journal toc kostenfrei https://doaj.org/toc/1735-7322 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 73 2015 2 127-131
language	Persian
source	In Tehran University Medical Journal 73(2015), 2, Seite 127-131 volume:73 year:2015 number:2 pages:127-131
sourceStr	In Tehran University Medical Journal 73(2015), 2, Seite 127-131 volume:73 year:2015 number:2 pages:127-131
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	CTLA-4 polymorphism genetics promoter regions systemic lupus erythematosus. Medicine (General)
isfreeaccess_bool	true
container_title	Tehran University Medical Journal
authorswithroles_txt_mv	Mahdieh Shojaa @@aut@@ Mehrdad Aghaie @@aut@@ Mahsa Amoli @@aut@@ Patricia Khashayar @@aut@@ Naemeh Javid @@aut@@ Fatemeh Shakeri @@aut@@ Mostafa Qorbani @@aut@@ Ramin Mohebbi @@aut@@
publishDateDaySort_date	2015-01-01T00:00:00Z
hierarchy_top_id	590956442
id	DOAJ050063375
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callnumber-first	R - Medicine
author	Mahdieh Shojaa
spellingShingle	Mahdieh Shojaa misc R5-920 misc CTLA-4 misc polymorphism misc genetics misc promoter regions misc systemic lupus erythematosus. misc Medicine (General) Association between CTLA-4 gene polymorphism and the risk of systemic lupus erythematosus: brief report
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topic_title	R5-920 Association between CTLA-4 gene polymorphism and the risk of systemic lupus erythematosus: brief report CTLA-4 polymorphism genetics promoter regions systemic lupus erythematosus
topic	misc R5-920 misc CTLA-4 misc polymorphism misc genetics misc promoter regions misc systemic lupus erythematosus. misc Medicine (General)
topic_unstemmed	misc R5-920 misc CTLA-4 misc polymorphism misc genetics misc promoter regions misc systemic lupus erythematosus. misc Medicine (General)
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title	Association between CTLA-4 gene polymorphism and the risk of systemic lupus erythematosus: brief report
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title_full	Association between CTLA-4 gene polymorphism and the risk of systemic lupus erythematosus: brief report
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author_browse	Mahdieh Shojaa Mehrdad Aghaie Mahsa Amoli Patricia Khashayar Naemeh Javid Fatemeh Shakeri Mostafa Qorbani Ramin Mohebbi
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title_sort	association between ctla-4 gene polymorphism and the risk of systemic lupus erythematosus: brief report
callnumber	R5-920
title_auth	Association between CTLA-4 gene polymorphism and the risk of systemic lupus erythematosus: brief report
abstract	Background: Cytotoxic lymphocyte antigen-4 (CTLA-4) plays an important role in regulating T cell activation. CTLA-4 gene polymorphisms are related with genetic susceptibility to various autoimmune diseases, including systemic lupus erythematosus (SLE). We analyzed the role of CTLA-4 polymorphisms at positions -318CT in patients who suffer from SLE. Methods: This study was performed on 180 SLE patients referred to 5th Azar University Hospital in Gorgan, Iran. Three hundred and four ethnically-and age-matched healthy controls with no history of autoimmune diseases entered the study between 5th May 2008 and 23rd October 2009. DNA was extracted from blood samples according to the standard procedure. Polymerase chain reaction- restriction fragments length polymorphism (PCR-RFLP) was used to analyze the genotype and allele frequencies of this polymorphism. PCR was carried out using the following primers: forward 5′-AAATGAATTGGACTGGATGGT-3′ and reverse 5′-TTACGAGAAAGGAAGCCGT G-3′. The frequency of alleles and genotypes were assessed using direct counting. Chi-square test and Fisher’s exact test were used to compare the association between the alleles and genotype frequencies and SLE. P<0.05 were considered statistically significant. Results: The CC genotype was observed in 94.5% of the SLE patients and 82.4% of the controls; the difference was statistically significant (P=0.0001, OR=3.51, CI95%=1.77-7.53). The CT genotype, on the other hand, was more frequently observed in the control group (17.1% vs. 5.5%, P=0.0001, OR=0.28). T allele was significantly more common in the controls compared to SLE patients (P=0.0001, OR=0.26, CI95%=0.13-0.53). Conclusion: Our results suggest that the -318C/T polymorphism of CTLA-4 gene might play a significant role in the genetic susceptibility to SLE. Therefore, further studies on populations, especially from other Middle East countries, are needed to confirm our results.
abstractGer	Background: Cytotoxic lymphocyte antigen-4 (CTLA-4) plays an important role in regulating T cell activation. CTLA-4 gene polymorphisms are related with genetic susceptibility to various autoimmune diseases, including systemic lupus erythematosus (SLE). We analyzed the role of CTLA-4 polymorphisms at positions -318CT in patients who suffer from SLE. Methods: This study was performed on 180 SLE patients referred to 5th Azar University Hospital in Gorgan, Iran. Three hundred and four ethnically-and age-matched healthy controls with no history of autoimmune diseases entered the study between 5th May 2008 and 23rd October 2009. DNA was extracted from blood samples according to the standard procedure. Polymerase chain reaction- restriction fragments length polymorphism (PCR-RFLP) was used to analyze the genotype and allele frequencies of this polymorphism. PCR was carried out using the following primers: forward 5′-AAATGAATTGGACTGGATGGT-3′ and reverse 5′-TTACGAGAAAGGAAGCCGT G-3′. The frequency of alleles and genotypes were assessed using direct counting. Chi-square test and Fisher’s exact test were used to compare the association between the alleles and genotype frequencies and SLE. P<0.05 were considered statistically significant. Results: The CC genotype was observed in 94.5% of the SLE patients and 82.4% of the controls; the difference was statistically significant (P=0.0001, OR=3.51, CI95%=1.77-7.53). The CT genotype, on the other hand, was more frequently observed in the control group (17.1% vs. 5.5%, P=0.0001, OR=0.28). T allele was significantly more common in the controls compared to SLE patients (P=0.0001, OR=0.26, CI95%=0.13-0.53). Conclusion: Our results suggest that the -318C/T polymorphism of CTLA-4 gene might play a significant role in the genetic susceptibility to SLE. Therefore, further studies on populations, especially from other Middle East countries, are needed to confirm our results.
abstract_unstemmed	Background: Cytotoxic lymphocyte antigen-4 (CTLA-4) plays an important role in regulating T cell activation. CTLA-4 gene polymorphisms are related with genetic susceptibility to various autoimmune diseases, including systemic lupus erythematosus (SLE). We analyzed the role of CTLA-4 polymorphisms at positions -318CT in patients who suffer from SLE. Methods: This study was performed on 180 SLE patients referred to 5th Azar University Hospital in Gorgan, Iran. Three hundred and four ethnically-and age-matched healthy controls with no history of autoimmune diseases entered the study between 5th May 2008 and 23rd October 2009. DNA was extracted from blood samples according to the standard procedure. Polymerase chain reaction- restriction fragments length polymorphism (PCR-RFLP) was used to analyze the genotype and allele frequencies of this polymorphism. PCR was carried out using the following primers: forward 5′-AAATGAATTGGACTGGATGGT-3′ and reverse 5′-TTACGAGAAAGGAAGCCGT G-3′. The frequency of alleles and genotypes were assessed using direct counting. Chi-square test and Fisher’s exact test were used to compare the association between the alleles and genotype frequencies and SLE. P<0.05 were considered statistically significant. Results: The CC genotype was observed in 94.5% of the SLE patients and 82.4% of the controls; the difference was statistically significant (P=0.0001, OR=3.51, CI95%=1.77-7.53). The CT genotype, on the other hand, was more frequently observed in the control group (17.1% vs. 5.5%, P=0.0001, OR=0.28). T allele was significantly more common in the controls compared to SLE patients (P=0.0001, OR=0.26, CI95%=0.13-0.53). Conclusion: Our results suggest that the -318C/T polymorphism of CTLA-4 gene might play a significant role in the genetic susceptibility to SLE. Therefore, further studies on populations, especially from other Middle East countries, are needed to confirm our results.
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title_short	Association between CTLA-4 gene polymorphism and the risk of systemic lupus erythematosus: brief report
url	https://doaj.org/article/c393d091fa3f4f2bbf750c01fd34d201 http://tumj.tums.ac.ir/browse.php?a_code=A-10-25-5326&slc_lang=en&sid=1 https://doaj.org/toc/1683-1764 https://doaj.org/toc/1735-7322
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author2	Mehrdad Aghaie Mahsa Amoli Patricia Khashayar Naemeh Javid Fatemeh Shakeri Mostafa Qorbani Ramin Mohebbi
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