The metabolically active bacterial microbiome of tonsils and mandibular lymph nodes of slaughter pigs

The exploration of microbiomes in lymphatic organs is relevant for basic and applied research into explaining microbial translocation processes and understanding cross-contamination during slaughter. This study aimed to investigate whether metabolically active bacteria (MAB) could be detected within...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Evelyne eMann [verfasserIn] Beate ePinior [verfasserIn] Stefanie eWetzels [verfasserIn] Barbara U. Metzler-Zebeli [verfasserIn] Martin eWagner [verfasserIn] Stephan eSchmitz-Esser [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2015

Schlagwörter:	microbiota Lymph Node pig tonsil contamination 16S rRNA sequencing

Übergeordnetes Werk:	In: Frontiers in Microbiology - Frontiers Media S.A., 2011, 6(2015)
Übergeordnetes Werk:	volume:6 ; year:2015

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fmicb.2015.01362

Katalog-ID:	DOAJ050608215

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spelling	10.3389/fmicb.2015.01362 doi (DE-627)DOAJ050608215 (DE-599)DOAJcc2ec4b0fcfa475a96c9d2c9f8e58b9a DE-627 ger DE-627 rakwb eng QR1-502 Evelyne eMann verfasserin aut The metabolically active bacterial microbiome of tonsils and mandibular lymph nodes of slaughter pigs 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The exploration of microbiomes in lymphatic organs is relevant for basic and applied research into explaining microbial translocation processes and understanding cross-contamination during slaughter. This study aimed to investigate whether metabolically active bacteria (MAB) could be detected within tonsils and mandibular lymph nodes (MLNs) of pigs. The hypervariable V1-V2 region of the bacterial 16S rRNA genes was amplified from cDNA from tonsils and MLNs of eight clinically healthy slaughter pigs. Pyrosequencing yielded 82,857 quality-controlled sequences, clustering into 576 operational taxonomic units (OTUs), which were assigned to 230 genera and 16 phyla. The actual number of detected OTUs per sample varied highly (23-171 OTUs). Prevotella zoogleoformans and Serratia proteamaculans (best type strain hits) were most abundant (10.6% and 41.8% respectively) in tonsils and MLNs, respectively. To explore bacterial correlation patterns between samples of each tissue, pairwise Spearman correlations (rs) were calculated. In total, 194 strong positive and negative correlations \|rs\| ≥ 0.6 were found. We conclude that (i) lymphatic organs harbor a high diversity of metabolically active bacteria, (ii) the occurrence of viable bacteria in lymph nodes is not restricted to pathological processes and (iii) lymphatic tissues may serve as a contamination source in pig slaughterhouses. This study confirms the necessity of the EFSA regulation with regard to a meat inspection based on visual examinations to foster a minimization of microbial contamination. microbiota Lymph Node pig tonsil contamination 16S rRNA sequencing Microbiology Beate ePinior verfasserin aut Stefanie eWetzels verfasserin aut Barbara U. Metzler-Zebeli verfasserin aut Martin eWagner verfasserin aut Stephan eSchmitz-Esser verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 6(2015) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:6 year:2015 https://doi.org/10.3389/fmicb.2015.01362 kostenfrei https://doaj.org/article/cc2ec4b0fcfa475a96c9d2c9f8e58b9a kostenfrei http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01362/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2015
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allfieldsGer	10.3389/fmicb.2015.01362 doi (DE-627)DOAJ050608215 (DE-599)DOAJcc2ec4b0fcfa475a96c9d2c9f8e58b9a DE-627 ger DE-627 rakwb eng QR1-502 Evelyne eMann verfasserin aut The metabolically active bacterial microbiome of tonsils and mandibular lymph nodes of slaughter pigs 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The exploration of microbiomes in lymphatic organs is relevant for basic and applied research into explaining microbial translocation processes and understanding cross-contamination during slaughter. This study aimed to investigate whether metabolically active bacteria (MAB) could be detected within tonsils and mandibular lymph nodes (MLNs) of pigs. The hypervariable V1-V2 region of the bacterial 16S rRNA genes was amplified from cDNA from tonsils and MLNs of eight clinically healthy slaughter pigs. Pyrosequencing yielded 82,857 quality-controlled sequences, clustering into 576 operational taxonomic units (OTUs), which were assigned to 230 genera and 16 phyla. The actual number of detected OTUs per sample varied highly (23-171 OTUs). Prevotella zoogleoformans and Serratia proteamaculans (best type strain hits) were most abundant (10.6% and 41.8% respectively) in tonsils and MLNs, respectively. To explore bacterial correlation patterns between samples of each tissue, pairwise Spearman correlations (rs) were calculated. In total, 194 strong positive and negative correlations \|rs\| ≥ 0.6 were found. We conclude that (i) lymphatic organs harbor a high diversity of metabolically active bacteria, (ii) the occurrence of viable bacteria in lymph nodes is not restricted to pathological processes and (iii) lymphatic tissues may serve as a contamination source in pig slaughterhouses. This study confirms the necessity of the EFSA regulation with regard to a meat inspection based on visual examinations to foster a minimization of microbial contamination. microbiota Lymph Node pig tonsil contamination 16S rRNA sequencing Microbiology Beate ePinior verfasserin aut Stefanie eWetzels verfasserin aut Barbara U. Metzler-Zebeli verfasserin aut Martin eWagner verfasserin aut Stephan eSchmitz-Esser verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 6(2015) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:6 year:2015 https://doi.org/10.3389/fmicb.2015.01362 kostenfrei https://doaj.org/article/cc2ec4b0fcfa475a96c9d2c9f8e58b9a kostenfrei http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01362/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2015
allfieldsSound	10.3389/fmicb.2015.01362 doi (DE-627)DOAJ050608215 (DE-599)DOAJcc2ec4b0fcfa475a96c9d2c9f8e58b9a DE-627 ger DE-627 rakwb eng QR1-502 Evelyne eMann verfasserin aut The metabolically active bacterial microbiome of tonsils and mandibular lymph nodes of slaughter pigs 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The exploration of microbiomes in lymphatic organs is relevant for basic and applied research into explaining microbial translocation processes and understanding cross-contamination during slaughter. This study aimed to investigate whether metabolically active bacteria (MAB) could be detected within tonsils and mandibular lymph nodes (MLNs) of pigs. The hypervariable V1-V2 region of the bacterial 16S rRNA genes was amplified from cDNA from tonsils and MLNs of eight clinically healthy slaughter pigs. Pyrosequencing yielded 82,857 quality-controlled sequences, clustering into 576 operational taxonomic units (OTUs), which were assigned to 230 genera and 16 phyla. The actual number of detected OTUs per sample varied highly (23-171 OTUs). Prevotella zoogleoformans and Serratia proteamaculans (best type strain hits) were most abundant (10.6% and 41.8% respectively) in tonsils and MLNs, respectively. To explore bacterial correlation patterns between samples of each tissue, pairwise Spearman correlations (rs) were calculated. In total, 194 strong positive and negative correlations \|rs\| ≥ 0.6 were found. We conclude that (i) lymphatic organs harbor a high diversity of metabolically active bacteria, (ii) the occurrence of viable bacteria in lymph nodes is not restricted to pathological processes and (iii) lymphatic tissues may serve as a contamination source in pig slaughterhouses. This study confirms the necessity of the EFSA regulation with regard to a meat inspection based on visual examinations to foster a minimization of microbial contamination. microbiota Lymph Node pig tonsil contamination 16S rRNA sequencing Microbiology Beate ePinior verfasserin aut Stefanie eWetzels verfasserin aut Barbara U. Metzler-Zebeli verfasserin aut Martin eWagner verfasserin aut Stephan eSchmitz-Esser verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 6(2015) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:6 year:2015 https://doi.org/10.3389/fmicb.2015.01362 kostenfrei https://doaj.org/article/cc2ec4b0fcfa475a96c9d2c9f8e58b9a kostenfrei http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01362/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2015
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authorswithroles_txt_mv	Evelyne eMann @@aut@@ Beate ePinior @@aut@@ Stefanie eWetzels @@aut@@ Barbara U. Metzler-Zebeli @@aut@@ Martin eWagner @@aut@@ Stephan eSchmitz-Esser @@aut@@
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callnumber-first	Q - Science
author	Evelyne eMann
spellingShingle	Evelyne eMann misc QR1-502 misc microbiota misc Lymph Node misc pig misc tonsil misc contamination misc 16S rRNA sequencing misc Microbiology The metabolically active bacterial microbiome of tonsils and mandibular lymph nodes of slaughter pigs
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topic_title	QR1-502 The metabolically active bacterial microbiome of tonsils and mandibular lymph nodes of slaughter pigs microbiota Lymph Node pig tonsil contamination 16S rRNA sequencing
topic	misc QR1-502 misc microbiota misc Lymph Node misc pig misc tonsil misc contamination misc 16S rRNA sequencing misc Microbiology
topic_unstemmed	misc QR1-502 misc microbiota misc Lymph Node misc pig misc tonsil misc contamination misc 16S rRNA sequencing misc Microbiology
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title	The metabolically active bacterial microbiome of tonsils and mandibular lymph nodes of slaughter pigs
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title_full	The metabolically active bacterial microbiome of tonsils and mandibular lymph nodes of slaughter pigs
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title_sort	metabolically active bacterial microbiome of tonsils and mandibular lymph nodes of slaughter pigs
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title_auth	The metabolically active bacterial microbiome of tonsils and mandibular lymph nodes of slaughter pigs
abstract	The exploration of microbiomes in lymphatic organs is relevant for basic and applied research into explaining microbial translocation processes and understanding cross-contamination during slaughter. This study aimed to investigate whether metabolically active bacteria (MAB) could be detected within tonsils and mandibular lymph nodes (MLNs) of pigs. The hypervariable V1-V2 region of the bacterial 16S rRNA genes was amplified from cDNA from tonsils and MLNs of eight clinically healthy slaughter pigs. Pyrosequencing yielded 82,857 quality-controlled sequences, clustering into 576 operational taxonomic units (OTUs), which were assigned to 230 genera and 16 phyla. The actual number of detected OTUs per sample varied highly (23-171 OTUs). Prevotella zoogleoformans and Serratia proteamaculans (best type strain hits) were most abundant (10.6% and 41.8% respectively) in tonsils and MLNs, respectively. To explore bacterial correlation patterns between samples of each tissue, pairwise Spearman correlations (rs) were calculated. In total, 194 strong positive and negative correlations \|rs\| ≥ 0.6 were found. We conclude that (i) lymphatic organs harbor a high diversity of metabolically active bacteria, (ii) the occurrence of viable bacteria in lymph nodes is not restricted to pathological processes and (iii) lymphatic tissues may serve as a contamination source in pig slaughterhouses. This study confirms the necessity of the EFSA regulation with regard to a meat inspection based on visual examinations to foster a minimization of microbial contamination.
abstractGer	The exploration of microbiomes in lymphatic organs is relevant for basic and applied research into explaining microbial translocation processes and understanding cross-contamination during slaughter. This study aimed to investigate whether metabolically active bacteria (MAB) could be detected within tonsils and mandibular lymph nodes (MLNs) of pigs. The hypervariable V1-V2 region of the bacterial 16S rRNA genes was amplified from cDNA from tonsils and MLNs of eight clinically healthy slaughter pigs. Pyrosequencing yielded 82,857 quality-controlled sequences, clustering into 576 operational taxonomic units (OTUs), which were assigned to 230 genera and 16 phyla. The actual number of detected OTUs per sample varied highly (23-171 OTUs). Prevotella zoogleoformans and Serratia proteamaculans (best type strain hits) were most abundant (10.6% and 41.8% respectively) in tonsils and MLNs, respectively. To explore bacterial correlation patterns between samples of each tissue, pairwise Spearman correlations (rs) were calculated. In total, 194 strong positive and negative correlations \|rs\| ≥ 0.6 were found. We conclude that (i) lymphatic organs harbor a high diversity of metabolically active bacteria, (ii) the occurrence of viable bacteria in lymph nodes is not restricted to pathological processes and (iii) lymphatic tissues may serve as a contamination source in pig slaughterhouses. This study confirms the necessity of the EFSA regulation with regard to a meat inspection based on visual examinations to foster a minimization of microbial contamination.
abstract_unstemmed	The exploration of microbiomes in lymphatic organs is relevant for basic and applied research into explaining microbial translocation processes and understanding cross-contamination during slaughter. This study aimed to investigate whether metabolically active bacteria (MAB) could be detected within tonsils and mandibular lymph nodes (MLNs) of pigs. The hypervariable V1-V2 region of the bacterial 16S rRNA genes was amplified from cDNA from tonsils and MLNs of eight clinically healthy slaughter pigs. Pyrosequencing yielded 82,857 quality-controlled sequences, clustering into 576 operational taxonomic units (OTUs), which were assigned to 230 genera and 16 phyla. The actual number of detected OTUs per sample varied highly (23-171 OTUs). Prevotella zoogleoformans and Serratia proteamaculans (best type strain hits) were most abundant (10.6% and 41.8% respectively) in tonsils and MLNs, respectively. To explore bacterial correlation patterns between samples of each tissue, pairwise Spearman correlations (rs) were calculated. In total, 194 strong positive and negative correlations \|rs\| ≥ 0.6 were found. We conclude that (i) lymphatic organs harbor a high diversity of metabolically active bacteria, (ii) the occurrence of viable bacteria in lymph nodes is not restricted to pathological processes and (iii) lymphatic tissues may serve as a contamination source in pig slaughterhouses. This study confirms the necessity of the EFSA regulation with regard to a meat inspection based on visual examinations to foster a minimization of microbial contamination.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
title_short	The metabolically active bacterial microbiome of tonsils and mandibular lymph nodes of slaughter pigs
url	https://doi.org/10.3389/fmicb.2015.01362 https://doaj.org/article/cc2ec4b0fcfa475a96c9d2c9f8e58b9a http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01362/full https://doaj.org/toc/1664-302X
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author2	Beate ePinior Stefanie eWetzels Barbara U. Metzler-Zebeli Martin eWagner Stephan eSchmitz-Esser
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up_date	2024-07-03T15:47:26.491Z
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The metabolically active bacterial microbiome of tonsils and mandibular lymph nodes of slaughter pigs

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