What Do We Have to Know about PD-L1 Expression in Prostate Cancer? A Systematic Literature Review. Part 1: Focus on Immunohistochemical Results with Discussion of Pre-Analytical and Interpretation Variables

Immunotherapy targeting the PD-1–PD-L1 axis yielded good results in treating different immunologically ‘‘hot’’ tumors. A phase II study revealed good therapeutic activity of pembrolizumab in selected prostatic carcinoma (PC)-patients. We performed a systematic literature review (PRISMA guidelines),...
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Autor*in:	Andrea Palicelli [verfasserIn] Martina Bonacini [verfasserIn] Stefania Croci [verfasserIn] Cristina Magi-Galluzzi [verfasserIn] Sofia Cañete-Portillo [verfasserIn] Alcides Chaux [verfasserIn] Alessandra Bisagni [verfasserIn] Eleonora Zanetti [verfasserIn] Dario De Biase [verfasserIn] Beatrice Melli [verfasserIn] Francesca Sanguedolce [verfasserIn] Moira Ragazzi [verfasserIn] Maria Paola Bonasoni [verfasserIn] Alessandra Soriano [verfasserIn] Stefano Ascani [verfasserIn] Maurizio Zizzo [verfasserIn] Carolina Castro Ruiz [verfasserIn] Antonio De Leo [verfasserIn] Guido Giordano [verfasserIn] Matteo Landriscina [verfasserIn] Giuseppe Carrieri [verfasserIn] Luigi Cormio [verfasserIn] Daniel M. Berney [verfasserIn] Daniel Athanazio [verfasserIn] Jatin Gandhi [verfasserIn] Alberto Cavazza [verfasserIn] Giacomo Santandrea [verfasserIn] Alessandro Tafuni [verfasserIn] Magda Zanelli [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	PD-L1 prostate cancer adenocarcinoma immunohistochemistry target-therapy

Übergeordnetes Werk:	In: Cells - MDPI AG, 2012, 10(2021), 11, p 3166
Übergeordnetes Werk:	volume:10 ; year:2021 ; number:11, p 3166

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/cells10113166

Katalog-ID:	DOAJ051241544

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allfields_unstemmed	10.3390/cells10113166 doi (DE-627)DOAJ051241544 (DE-599)DOAJbb8e29accbd74f43b44ae3429430e2c6 DE-627 ger DE-627 rakwb eng QH573-671 Andrea Palicelli verfasserin aut What Do We Have to Know about PD-L1 Expression in Prostate Cancer? A Systematic Literature Review. Part 1: Focus on Immunohistochemical Results with Discussion of Pre-Analytical and Interpretation Variables 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Immunotherapy targeting the PD-1–PD-L1 axis yielded good results in treating different immunologically ‘‘hot’’ tumors. A phase II study revealed good therapeutic activity of pembrolizumab in selected prostatic carcinoma (PC)-patients. We performed a systematic literature review (PRISMA guidelines), which analyzes the immunohistochemical expression of PD-L1 in human PC samples and highlights the pre-analytical and interpretation variables. Interestingly, 29% acinar PCs, 7% ductal PCs, and 46% neuroendocrine carcinomas/tumors were PD-L1+ on immunohistochemistry. Different scoring methods or cut-off criteria were applied on variable specimen-types, evaluating tumors showing different clinic-pathologic features. The positivity rate of different PD-L1 antibody clones in tumor cells ranged from 3% (SP142) to 50% (ABM4E54), excluding the single case tested for RM-320. The most tested clone was E1L3N, followed by 22C3 (most used for pembrolizumab eligibility), SP263, SP142, and 28-8, which gave the positivity rates of 35%, 11–41% (depending on different scoring systems), 6%, 3%, and 15%, respectively. Other clones were tested in <200 cases. The PD-L1 positivity rate was usually higher in tumors than benign tissues. It was higher in non-tissue microarray specimens (41–50% vs. 15%), as PC cells frequently showed heterogenous or focal PD-L1-staining. PD-L1 was expressed by immune or stromal cells in 12% and 69% cases, respectively. Tumor heterogeneity, inter-institutional preanalytics, and inter-observer interpretation variability may account for result biases. PD-L1 prostate cancer adenocarcinoma immunohistochemistry target-therapy Cytology Martina Bonacini verfasserin aut Stefania Croci verfasserin aut Cristina Magi-Galluzzi verfasserin aut Sofia Cañete-Portillo verfasserin aut Alcides Chaux verfasserin aut Alessandra Bisagni verfasserin aut Eleonora Zanetti verfasserin aut Dario De Biase verfasserin aut Beatrice Melli verfasserin aut Francesca Sanguedolce verfasserin aut Moira Ragazzi verfasserin aut Maria Paola Bonasoni verfasserin aut Alessandra Soriano verfasserin aut Stefano Ascani verfasserin aut Maurizio Zizzo verfasserin aut Carolina Castro Ruiz verfasserin aut Antonio De Leo verfasserin aut Guido Giordano verfasserin aut Matteo Landriscina verfasserin aut Giuseppe Carrieri verfasserin aut Luigi Cormio verfasserin aut Daniel M. Berney verfasserin aut Daniel Athanazio verfasserin aut Jatin Gandhi verfasserin aut Alberto Cavazza verfasserin aut Giacomo Santandrea verfasserin aut Alessandro Tafuni verfasserin aut Magda Zanelli verfasserin aut In Cells MDPI AG, 2012 10(2021), 11, p 3166 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:10 year:2021 number:11, p 3166 https://doi.org/10.3390/cells10113166 kostenfrei https://doaj.org/article/bb8e29accbd74f43b44ae3429430e2c6 kostenfrei https://www.mdpi.com/2073-4409/10/11/3166 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 11, p 3166
allfieldsGer	10.3390/cells10113166 doi (DE-627)DOAJ051241544 (DE-599)DOAJbb8e29accbd74f43b44ae3429430e2c6 DE-627 ger DE-627 rakwb eng QH573-671 Andrea Palicelli verfasserin aut What Do We Have to Know about PD-L1 Expression in Prostate Cancer? A Systematic Literature Review. Part 1: Focus on Immunohistochemical Results with Discussion of Pre-Analytical and Interpretation Variables 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Immunotherapy targeting the PD-1–PD-L1 axis yielded good results in treating different immunologically ‘‘hot’’ tumors. A phase II study revealed good therapeutic activity of pembrolizumab in selected prostatic carcinoma (PC)-patients. We performed a systematic literature review (PRISMA guidelines), which analyzes the immunohistochemical expression of PD-L1 in human PC samples and highlights the pre-analytical and interpretation variables. Interestingly, 29% acinar PCs, 7% ductal PCs, and 46% neuroendocrine carcinomas/tumors were PD-L1+ on immunohistochemistry. Different scoring methods or cut-off criteria were applied on variable specimen-types, evaluating tumors showing different clinic-pathologic features. The positivity rate of different PD-L1 antibody clones in tumor cells ranged from 3% (SP142) to 50% (ABM4E54), excluding the single case tested for RM-320. The most tested clone was E1L3N, followed by 22C3 (most used for pembrolizumab eligibility), SP263, SP142, and 28-8, which gave the positivity rates of 35%, 11–41% (depending on different scoring systems), 6%, 3%, and 15%, respectively. Other clones were tested in <200 cases. The PD-L1 positivity rate was usually higher in tumors than benign tissues. It was higher in non-tissue microarray specimens (41–50% vs. 15%), as PC cells frequently showed heterogenous or focal PD-L1-staining. PD-L1 was expressed by immune or stromal cells in 12% and 69% cases, respectively. Tumor heterogeneity, inter-institutional preanalytics, and inter-observer interpretation variability may account for result biases. PD-L1 prostate cancer adenocarcinoma immunohistochemistry target-therapy Cytology Martina Bonacini verfasserin aut Stefania Croci verfasserin aut Cristina Magi-Galluzzi verfasserin aut Sofia Cañete-Portillo verfasserin aut Alcides Chaux verfasserin aut Alessandra Bisagni verfasserin aut Eleonora Zanetti verfasserin aut Dario De Biase verfasserin aut Beatrice Melli verfasserin aut Francesca Sanguedolce verfasserin aut Moira Ragazzi verfasserin aut Maria Paola Bonasoni verfasserin aut Alessandra Soriano verfasserin aut Stefano Ascani verfasserin aut Maurizio Zizzo verfasserin aut Carolina Castro Ruiz verfasserin aut Antonio De Leo verfasserin aut Guido Giordano verfasserin aut Matteo Landriscina verfasserin aut Giuseppe Carrieri verfasserin aut Luigi Cormio verfasserin aut Daniel M. Berney verfasserin aut Daniel Athanazio verfasserin aut Jatin Gandhi verfasserin aut Alberto Cavazza verfasserin aut Giacomo Santandrea verfasserin aut Alessandro Tafuni verfasserin aut Magda Zanelli verfasserin aut In Cells MDPI AG, 2012 10(2021), 11, p 3166 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:10 year:2021 number:11, p 3166 https://doi.org/10.3390/cells10113166 kostenfrei https://doaj.org/article/bb8e29accbd74f43b44ae3429430e2c6 kostenfrei https://www.mdpi.com/2073-4409/10/11/3166 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 11, p 3166
allfieldsSound	10.3390/cells10113166 doi (DE-627)DOAJ051241544 (DE-599)DOAJbb8e29accbd74f43b44ae3429430e2c6 DE-627 ger DE-627 rakwb eng QH573-671 Andrea Palicelli verfasserin aut What Do We Have to Know about PD-L1 Expression in Prostate Cancer? A Systematic Literature Review. Part 1: Focus on Immunohistochemical Results with Discussion of Pre-Analytical and Interpretation Variables 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Immunotherapy targeting the PD-1–PD-L1 axis yielded good results in treating different immunologically ‘‘hot’’ tumors. A phase II study revealed good therapeutic activity of pembrolizumab in selected prostatic carcinoma (PC)-patients. We performed a systematic literature review (PRISMA guidelines), which analyzes the immunohistochemical expression of PD-L1 in human PC samples and highlights the pre-analytical and interpretation variables. Interestingly, 29% acinar PCs, 7% ductal PCs, and 46% neuroendocrine carcinomas/tumors were PD-L1+ on immunohistochemistry. Different scoring methods or cut-off criteria were applied on variable specimen-types, evaluating tumors showing different clinic-pathologic features. The positivity rate of different PD-L1 antibody clones in tumor cells ranged from 3% (SP142) to 50% (ABM4E54), excluding the single case tested for RM-320. The most tested clone was E1L3N, followed by 22C3 (most used for pembrolizumab eligibility), SP263, SP142, and 28-8, which gave the positivity rates of 35%, 11–41% (depending on different scoring systems), 6%, 3%, and 15%, respectively. Other clones were tested in <200 cases. The PD-L1 positivity rate was usually higher in tumors than benign tissues. It was higher in non-tissue microarray specimens (41–50% vs. 15%), as PC cells frequently showed heterogenous or focal PD-L1-staining. PD-L1 was expressed by immune or stromal cells in 12% and 69% cases, respectively. Tumor heterogeneity, inter-institutional preanalytics, and inter-observer interpretation variability may account for result biases. PD-L1 prostate cancer adenocarcinoma immunohistochemistry target-therapy Cytology Martina Bonacini verfasserin aut Stefania Croci verfasserin aut Cristina Magi-Galluzzi verfasserin aut Sofia Cañete-Portillo verfasserin aut Alcides Chaux verfasserin aut Alessandra Bisagni verfasserin aut Eleonora Zanetti verfasserin aut Dario De Biase verfasserin aut Beatrice Melli verfasserin aut Francesca Sanguedolce verfasserin aut Moira Ragazzi verfasserin aut Maria Paola Bonasoni verfasserin aut Alessandra Soriano verfasserin aut Stefano Ascani verfasserin aut Maurizio Zizzo verfasserin aut Carolina Castro Ruiz verfasserin aut Antonio De Leo verfasserin aut Guido Giordano verfasserin aut Matteo Landriscina verfasserin aut Giuseppe Carrieri verfasserin aut Luigi Cormio verfasserin aut Daniel M. Berney verfasserin aut Daniel Athanazio verfasserin aut Jatin Gandhi verfasserin aut Alberto Cavazza verfasserin aut Giacomo Santandrea verfasserin aut Alessandro Tafuni verfasserin aut Magda Zanelli verfasserin aut In Cells MDPI AG, 2012 10(2021), 11, p 3166 (DE-627)718622081 (DE-600)2661518-6 20734409 nnns volume:10 year:2021 number:11, p 3166 https://doi.org/10.3390/cells10113166 kostenfrei https://doaj.org/article/bb8e29accbd74f43b44ae3429430e2c6 kostenfrei https://www.mdpi.com/2073-4409/10/11/3166 kostenfrei https://doaj.org/toc/2073-4409 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 11, p 3166
language	English
source	In Cells 10(2021), 11, p 3166 volume:10 year:2021 number:11, p 3166
sourceStr	In Cells 10(2021), 11, p 3166 volume:10 year:2021 number:11, p 3166
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	PD-L1 prostate cancer adenocarcinoma immunohistochemistry target-therapy Cytology
isfreeaccess_bool	true
container_title	Cells
authorswithroles_txt_mv	Andrea Palicelli @@aut@@ Martina Bonacini @@aut@@ Stefania Croci @@aut@@ Cristina Magi-Galluzzi @@aut@@ Sofia Cañete-Portillo @@aut@@ Alcides Chaux @@aut@@ Alessandra Bisagni @@aut@@ Eleonora Zanetti @@aut@@ Dario De Biase @@aut@@ Beatrice Melli @@aut@@ Francesca Sanguedolce @@aut@@ Moira Ragazzi @@aut@@ Maria Paola Bonasoni @@aut@@ Alessandra Soriano @@aut@@ Stefano Ascani @@aut@@ Maurizio Zizzo @@aut@@ Carolina Castro Ruiz @@aut@@ Antonio De Leo @@aut@@ Guido Giordano @@aut@@ Matteo Landriscina @@aut@@ Giuseppe Carrieri @@aut@@ Luigi Cormio @@aut@@ Daniel M. Berney @@aut@@ Daniel Athanazio @@aut@@ Jatin Gandhi @@aut@@ Alberto Cavazza @@aut@@ Giacomo Santandrea @@aut@@ Alessandro Tafuni @@aut@@ Magda Zanelli @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	718622081
id	DOAJ051241544
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ051241544</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20240412132651.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230227s2021 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.3390/cells10113166</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ051241544</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJbb8e29accbd74f43b44ae3429430e2c6</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QH573-671</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Andrea Palicelli</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">What Do We Have to Know about PD-L1 Expression in Prostate Cancer? A Systematic Literature Review. Part 1: Focus on Immunohistochemical Results with Discussion of Pre-Analytical and Interpretation Variables</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2021</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Immunotherapy targeting the PD-1–PD-L1 axis yielded good results in treating different immunologically ‘‘hot’’ tumors. 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callnumber-first	Q - Science
author	Andrea Palicelli
spellingShingle	Andrea Palicelli misc QH573-671 misc PD-L1 misc prostate misc cancer misc adenocarcinoma misc immunohistochemistry misc target-therapy misc Cytology What Do We Have to Know about PD-L1 Expression in Prostate Cancer? A Systematic Literature Review. Part 1: Focus on Immunohistochemical Results with Discussion of Pre-Analytical and Interpretation Variables
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topic_title	QH573-671 What Do We Have to Know about PD-L1 Expression in Prostate Cancer? A Systematic Literature Review. Part 1: Focus on Immunohistochemical Results with Discussion of Pre-Analytical and Interpretation Variables PD-L1 prostate cancer adenocarcinoma immunohistochemistry target-therapy
topic	misc QH573-671 misc PD-L1 misc prostate misc cancer misc adenocarcinoma misc immunohistochemistry misc target-therapy misc Cytology
topic_unstemmed	misc QH573-671 misc PD-L1 misc prostate misc cancer misc adenocarcinoma misc immunohistochemistry misc target-therapy misc Cytology
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title	What Do We Have to Know about PD-L1 Expression in Prostate Cancer? A Systematic Literature Review. Part 1: Focus on Immunohistochemical Results with Discussion of Pre-Analytical and Interpretation Variables
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title_full	What Do We Have to Know about PD-L1 Expression in Prostate Cancer? A Systematic Literature Review. Part 1: Focus on Immunohistochemical Results with Discussion of Pre-Analytical and Interpretation Variables
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title_sort	what do we have to know about pd-l1 expression in prostate cancer? a systematic literature review. part 1: focus on immunohistochemical results with discussion of pre-analytical and interpretation variables
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title_auth	What Do We Have to Know about PD-L1 Expression in Prostate Cancer? A Systematic Literature Review. Part 1: Focus on Immunohistochemical Results with Discussion of Pre-Analytical and Interpretation Variables
abstract	Immunotherapy targeting the PD-1–PD-L1 axis yielded good results in treating different immunologically ‘‘hot’’ tumors. A phase II study revealed good therapeutic activity of pembrolizumab in selected prostatic carcinoma (PC)-patients. We performed a systematic literature review (PRISMA guidelines), which analyzes the immunohistochemical expression of PD-L1 in human PC samples and highlights the pre-analytical and interpretation variables. Interestingly, 29% acinar PCs, 7% ductal PCs, and 46% neuroendocrine carcinomas/tumors were PD-L1+ on immunohistochemistry. Different scoring methods or cut-off criteria were applied on variable specimen-types, evaluating tumors showing different clinic-pathologic features. The positivity rate of different PD-L1 antibody clones in tumor cells ranged from 3% (SP142) to 50% (ABM4E54), excluding the single case tested for RM-320. The most tested clone was E1L3N, followed by 22C3 (most used for pembrolizumab eligibility), SP263, SP142, and 28-8, which gave the positivity rates of 35%, 11–41% (depending on different scoring systems), 6%, 3%, and 15%, respectively. Other clones were tested in <200 cases. The PD-L1 positivity rate was usually higher in tumors than benign tissues. It was higher in non-tissue microarray specimens (41–50% vs. 15%), as PC cells frequently showed heterogenous or focal PD-L1-staining. PD-L1 was expressed by immune or stromal cells in 12% and 69% cases, respectively. Tumor heterogeneity, inter-institutional preanalytics, and inter-observer interpretation variability may account for result biases.
abstractGer	Immunotherapy targeting the PD-1–PD-L1 axis yielded good results in treating different immunologically ‘‘hot’’ tumors. A phase II study revealed good therapeutic activity of pembrolizumab in selected prostatic carcinoma (PC)-patients. We performed a systematic literature review (PRISMA guidelines), which analyzes the immunohistochemical expression of PD-L1 in human PC samples and highlights the pre-analytical and interpretation variables. Interestingly, 29% acinar PCs, 7% ductal PCs, and 46% neuroendocrine carcinomas/tumors were PD-L1+ on immunohistochemistry. Different scoring methods or cut-off criteria were applied on variable specimen-types, evaluating tumors showing different clinic-pathologic features. The positivity rate of different PD-L1 antibody clones in tumor cells ranged from 3% (SP142) to 50% (ABM4E54), excluding the single case tested for RM-320. The most tested clone was E1L3N, followed by 22C3 (most used for pembrolizumab eligibility), SP263, SP142, and 28-8, which gave the positivity rates of 35%, 11–41% (depending on different scoring systems), 6%, 3%, and 15%, respectively. Other clones were tested in <200 cases. The PD-L1 positivity rate was usually higher in tumors than benign tissues. It was higher in non-tissue microarray specimens (41–50% vs. 15%), as PC cells frequently showed heterogenous or focal PD-L1-staining. PD-L1 was expressed by immune or stromal cells in 12% and 69% cases, respectively. Tumor heterogeneity, inter-institutional preanalytics, and inter-observer interpretation variability may account for result biases.
abstract_unstemmed	Immunotherapy targeting the PD-1–PD-L1 axis yielded good results in treating different immunologically ‘‘hot’’ tumors. A phase II study revealed good therapeutic activity of pembrolizumab in selected prostatic carcinoma (PC)-patients. We performed a systematic literature review (PRISMA guidelines), which analyzes the immunohistochemical expression of PD-L1 in human PC samples and highlights the pre-analytical and interpretation variables. Interestingly, 29% acinar PCs, 7% ductal PCs, and 46% neuroendocrine carcinomas/tumors were PD-L1+ on immunohistochemistry. Different scoring methods or cut-off criteria were applied on variable specimen-types, evaluating tumors showing different clinic-pathologic features. The positivity rate of different PD-L1 antibody clones in tumor cells ranged from 3% (SP142) to 50% (ABM4E54), excluding the single case tested for RM-320. The most tested clone was E1L3N, followed by 22C3 (most used for pembrolizumab eligibility), SP263, SP142, and 28-8, which gave the positivity rates of 35%, 11–41% (depending on different scoring systems), 6%, 3%, and 15%, respectively. Other clones were tested in <200 cases. The PD-L1 positivity rate was usually higher in tumors than benign tissues. It was higher in non-tissue microarray specimens (41–50% vs. 15%), as PC cells frequently showed heterogenous or focal PD-L1-staining. PD-L1 was expressed by immune or stromal cells in 12% and 69% cases, respectively. Tumor heterogeneity, inter-institutional preanalytics, and inter-observer interpretation variability may account for result biases.
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title_short	What Do We Have to Know about PD-L1 Expression in Prostate Cancer? A Systematic Literature Review. Part 1: Focus on Immunohistochemical Results with Discussion of Pre-Analytical and Interpretation Variables
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up_date	2024-07-03T19:11:59.079Z
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fullrecord_marcxml	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ051241544</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20240412132651.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230227s2021 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.3390/cells10113166</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ051241544</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJbb8e29accbd74f43b44ae3429430e2c6</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QH573-671</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Andrea Palicelli</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">What Do We Have to Know about PD-L1 Expression in Prostate Cancer? A Systematic Literature Review. Part 1: Focus on Immunohistochemical Results with Discussion of Pre-Analytical and Interpretation Variables</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2021</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Immunotherapy targeting the PD-1–PD-L1 axis yielded good results in treating different immunologically ‘‘hot’’ tumors. A phase II study revealed good therapeutic activity of pembrolizumab in selected prostatic carcinoma (PC)-patients. We performed a systematic literature review (PRISMA guidelines), which analyzes the immunohistochemical expression of PD-L1 in human PC samples and highlights the pre-analytical and interpretation variables. Interestingly, 29% acinar PCs, 7% ductal PCs, and 46% neuroendocrine carcinomas/tumors were PD-L1+ on immunohistochemistry. Different scoring methods or cut-off criteria were applied on variable specimen-types, evaluating tumors showing different clinic-pathologic features. The positivity rate of different PD-L1 antibody clones in tumor cells ranged from 3% (SP142) to 50% (ABM4E54), excluding the single case tested for RM-320. The most tested clone was E1L3N, followed by 22C3 (most used for pembrolizumab eligibility), SP263, SP142, and 28-8, which gave the positivity rates of 35%, 11–41% (depending on different scoring systems), 6%, 3%, and 15%, respectively. Other clones were tested in <200 cases. The PD-L1 positivity rate was usually higher in tumors than benign tissues. It was higher in non-tissue microarray specimens (41–50% vs. 15%), as PC cells frequently showed heterogenous or focal PD-L1-staining. PD-L1 was expressed by immune or stromal cells in 12% and 69% cases, respectively. 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What Do We Have to Know about PD-L1 Expression in Prostate Cancer? A Systematic Literature Review. Part 1: Focus on Immunohistochemical Results with Discussion of Pre-Analytical and Interpretation Variables

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