Identification and Functional Characterization of Tissue-Specific Terpene Synthases in <i<Stevia rebaudiana</i<

In addition to the well-known diterpenoid steviol glycosides, <i<Stevia rebaudiana</i< (Stevia) produces many labdane-type diterpenoids and a wide range of mono- and sesquiterpenoids. However, biosynthesis of mono- and sesquiterpenoids in Stevia remains unknown. Here we analyzed the extr...
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Autor*in:	Savitha Dhandapani [verfasserIn] Mi Jung Kim [verfasserIn] Hui Jun Chin [verfasserIn] Sing Hui Leong [verfasserIn] In-Cheol Jang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	stevia terpenoids terpene synthase volatile organic compounds

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 21(2020), 22, p 8566
Übergeordnetes Werk:	volume:21 ; year:2020 ; number:22, p 8566

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms21228566

Katalog-ID:	DOAJ051446294

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allfields	10.3390/ijms21228566 doi (DE-627)DOAJ051446294 (DE-599)DOAJbba48014524a4918a65e2bc2a8daea83 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Savitha Dhandapani verfasserin aut Identification and Functional Characterization of Tissue-Specific Terpene Synthases in <i<Stevia rebaudiana</i< 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In addition to the well-known diterpenoid steviol glycosides, <i<Stevia rebaudiana</i< (Stevia) produces many labdane-type diterpenoids and a wide range of mono- and sesquiterpenoids. However, biosynthesis of mono- and sesquiterpenoids in Stevia remains unknown. Here we analyzed the extracts of Stevia leaves, flowers, stems, and roots by Gas Chromatography–Mass Spectrometry and putatively identified a total of 69 volatile organic compounds, most of which were terpenoids with considerably varied quantities among the four tissues of Stevia. Using Stevia transcriptomes, we identified and functionally characterized five terpene synthases (TPSs) that produced major mono- and sesquiterpenoids in Stevia. Transcript levels of these Stevia <i<TPS</i<s and levels of corresponding terpenoids correlated well in Stevia tissues. Particularly, the root-specific SrTPS4 and SrTPS5 catalyzed the formation of γ-curcumene/zingiberene/β-sesquiphellandrene and α-longipinene/β-himachalene/himachalol as multifunctional sesqui-TPSs, respectively. Most of the <i<SrTPSs</i< were highly responsive to various environmental stresses in a tissue-specific manner. Taken together, our results provide new insights into how Stevia produces diverse terpenoids to confer differential responses to various environmental factors in each tissue. stevia <i<Stevia rebaudiana</i< terpenoids terpene synthase volatile organic compounds Biology (General) Chemistry Mi Jung Kim verfasserin aut Hui Jun Chin verfasserin aut Sing Hui Leong verfasserin aut In-Cheol Jang verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 21(2020), 22, p 8566 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:21 year:2020 number:22, p 8566 https://doi.org/10.3390/ijms21228566 kostenfrei https://doaj.org/article/bba48014524a4918a65e2bc2a8daea83 kostenfrei https://www.mdpi.com/1422-0067/21/22/8566 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2020 22, p 8566
spelling	10.3390/ijms21228566 doi (DE-627)DOAJ051446294 (DE-599)DOAJbba48014524a4918a65e2bc2a8daea83 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Savitha Dhandapani verfasserin aut Identification and Functional Characterization of Tissue-Specific Terpene Synthases in <i<Stevia rebaudiana</i< 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In addition to the well-known diterpenoid steviol glycosides, <i<Stevia rebaudiana</i< (Stevia) produces many labdane-type diterpenoids and a wide range of mono- and sesquiterpenoids. However, biosynthesis of mono- and sesquiterpenoids in Stevia remains unknown. Here we analyzed the extracts of Stevia leaves, flowers, stems, and roots by Gas Chromatography–Mass Spectrometry and putatively identified a total of 69 volatile organic compounds, most of which were terpenoids with considerably varied quantities among the four tissues of Stevia. Using Stevia transcriptomes, we identified and functionally characterized five terpene synthases (TPSs) that produced major mono- and sesquiterpenoids in Stevia. Transcript levels of these Stevia <i<TPS</i<s and levels of corresponding terpenoids correlated well in Stevia tissues. Particularly, the root-specific SrTPS4 and SrTPS5 catalyzed the formation of γ-curcumene/zingiberene/β-sesquiphellandrene and α-longipinene/β-himachalene/himachalol as multifunctional sesqui-TPSs, respectively. Most of the <i<SrTPSs</i< were highly responsive to various environmental stresses in a tissue-specific manner. Taken together, our results provide new insights into how Stevia produces diverse terpenoids to confer differential responses to various environmental factors in each tissue. stevia <i<Stevia rebaudiana</i< terpenoids terpene synthase volatile organic compounds Biology (General) Chemistry Mi Jung Kim verfasserin aut Hui Jun Chin verfasserin aut Sing Hui Leong verfasserin aut In-Cheol Jang verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 21(2020), 22, p 8566 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:21 year:2020 number:22, p 8566 https://doi.org/10.3390/ijms21228566 kostenfrei https://doaj.org/article/bba48014524a4918a65e2bc2a8daea83 kostenfrei https://www.mdpi.com/1422-0067/21/22/8566 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2020 22, p 8566
allfields_unstemmed	10.3390/ijms21228566 doi (DE-627)DOAJ051446294 (DE-599)DOAJbba48014524a4918a65e2bc2a8daea83 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Savitha Dhandapani verfasserin aut Identification and Functional Characterization of Tissue-Specific Terpene Synthases in <i<Stevia rebaudiana</i< 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In addition to the well-known diterpenoid steviol glycosides, <i<Stevia rebaudiana</i< (Stevia) produces many labdane-type diterpenoids and a wide range of mono- and sesquiterpenoids. However, biosynthesis of mono- and sesquiterpenoids in Stevia remains unknown. Here we analyzed the extracts of Stevia leaves, flowers, stems, and roots by Gas Chromatography–Mass Spectrometry and putatively identified a total of 69 volatile organic compounds, most of which were terpenoids with considerably varied quantities among the four tissues of Stevia. Using Stevia transcriptomes, we identified and functionally characterized five terpene synthases (TPSs) that produced major mono- and sesquiterpenoids in Stevia. Transcript levels of these Stevia <i<TPS</i<s and levels of corresponding terpenoids correlated well in Stevia tissues. Particularly, the root-specific SrTPS4 and SrTPS5 catalyzed the formation of γ-curcumene/zingiberene/β-sesquiphellandrene and α-longipinene/β-himachalene/himachalol as multifunctional sesqui-TPSs, respectively. Most of the <i<SrTPSs</i< were highly responsive to various environmental stresses in a tissue-specific manner. Taken together, our results provide new insights into how Stevia produces diverse terpenoids to confer differential responses to various environmental factors in each tissue. stevia <i<Stevia rebaudiana</i< terpenoids terpene synthase volatile organic compounds Biology (General) Chemistry Mi Jung Kim verfasserin aut Hui Jun Chin verfasserin aut Sing Hui Leong verfasserin aut In-Cheol Jang verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 21(2020), 22, p 8566 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:21 year:2020 number:22, p 8566 https://doi.org/10.3390/ijms21228566 kostenfrei https://doaj.org/article/bba48014524a4918a65e2bc2a8daea83 kostenfrei https://www.mdpi.com/1422-0067/21/22/8566 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2020 22, p 8566
allfieldsGer	10.3390/ijms21228566 doi (DE-627)DOAJ051446294 (DE-599)DOAJbba48014524a4918a65e2bc2a8daea83 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Savitha Dhandapani verfasserin aut Identification and Functional Characterization of Tissue-Specific Terpene Synthases in <i<Stevia rebaudiana</i< 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In addition to the well-known diterpenoid steviol glycosides, <i<Stevia rebaudiana</i< (Stevia) produces many labdane-type diterpenoids and a wide range of mono- and sesquiterpenoids. However, biosynthesis of mono- and sesquiterpenoids in Stevia remains unknown. Here we analyzed the extracts of Stevia leaves, flowers, stems, and roots by Gas Chromatography–Mass Spectrometry and putatively identified a total of 69 volatile organic compounds, most of which were terpenoids with considerably varied quantities among the four tissues of Stevia. Using Stevia transcriptomes, we identified and functionally characterized five terpene synthases (TPSs) that produced major mono- and sesquiterpenoids in Stevia. Transcript levels of these Stevia <i<TPS</i<s and levels of corresponding terpenoids correlated well in Stevia tissues. Particularly, the root-specific SrTPS4 and SrTPS5 catalyzed the formation of γ-curcumene/zingiberene/β-sesquiphellandrene and α-longipinene/β-himachalene/himachalol as multifunctional sesqui-TPSs, respectively. Most of the <i<SrTPSs</i< were highly responsive to various environmental stresses in a tissue-specific manner. Taken together, our results provide new insights into how Stevia produces diverse terpenoids to confer differential responses to various environmental factors in each tissue. stevia <i<Stevia rebaudiana</i< terpenoids terpene synthase volatile organic compounds Biology (General) Chemistry Mi Jung Kim verfasserin aut Hui Jun Chin verfasserin aut Sing Hui Leong verfasserin aut In-Cheol Jang verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 21(2020), 22, p 8566 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:21 year:2020 number:22, p 8566 https://doi.org/10.3390/ijms21228566 kostenfrei https://doaj.org/article/bba48014524a4918a65e2bc2a8daea83 kostenfrei https://www.mdpi.com/1422-0067/21/22/8566 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2020 22, p 8566
allfieldsSound	10.3390/ijms21228566 doi (DE-627)DOAJ051446294 (DE-599)DOAJbba48014524a4918a65e2bc2a8daea83 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Savitha Dhandapani verfasserin aut Identification and Functional Characterization of Tissue-Specific Terpene Synthases in <i<Stevia rebaudiana</i< 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In addition to the well-known diterpenoid steviol glycosides, <i<Stevia rebaudiana</i< (Stevia) produces many labdane-type diterpenoids and a wide range of mono- and sesquiterpenoids. However, biosynthesis of mono- and sesquiterpenoids in Stevia remains unknown. Here we analyzed the extracts of Stevia leaves, flowers, stems, and roots by Gas Chromatography–Mass Spectrometry and putatively identified a total of 69 volatile organic compounds, most of which were terpenoids with considerably varied quantities among the four tissues of Stevia. Using Stevia transcriptomes, we identified and functionally characterized five terpene synthases (TPSs) that produced major mono- and sesquiterpenoids in Stevia. Transcript levels of these Stevia <i<TPS</i<s and levels of corresponding terpenoids correlated well in Stevia tissues. Particularly, the root-specific SrTPS4 and SrTPS5 catalyzed the formation of γ-curcumene/zingiberene/β-sesquiphellandrene and α-longipinene/β-himachalene/himachalol as multifunctional sesqui-TPSs, respectively. Most of the <i<SrTPSs</i< were highly responsive to various environmental stresses in a tissue-specific manner. Taken together, our results provide new insights into how Stevia produces diverse terpenoids to confer differential responses to various environmental factors in each tissue. stevia <i<Stevia rebaudiana</i< terpenoids terpene synthase volatile organic compounds Biology (General) Chemistry Mi Jung Kim verfasserin aut Hui Jun Chin verfasserin aut Sing Hui Leong verfasserin aut In-Cheol Jang verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 21(2020), 22, p 8566 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:21 year:2020 number:22, p 8566 https://doi.org/10.3390/ijms21228566 kostenfrei https://doaj.org/article/bba48014524a4918a65e2bc2a8daea83 kostenfrei https://www.mdpi.com/1422-0067/21/22/8566 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2020 22, p 8566
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callnumber-first	Q - Science
author	Savitha Dhandapani
spellingShingle	Savitha Dhandapani misc QH301-705.5 misc QD1-999 misc stevia misc <i<Stevia rebaudiana</i< misc terpenoids misc terpene synthase misc volatile organic compounds misc Biology (General) misc Chemistry Identification and Functional Characterization of Tissue-Specific Terpene Synthases in <i<Stevia rebaudiana</i<
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topic_title	QH301-705.5 QD1-999 Identification and Functional Characterization of Tissue-Specific Terpene Synthases in <i<Stevia rebaudiana</i< stevia <i<Stevia rebaudiana</i< terpenoids terpene synthase volatile organic compounds
topic	misc QH301-705.5 misc QD1-999 misc stevia misc <i<Stevia rebaudiana</i< misc terpenoids misc terpene synthase misc volatile organic compounds misc Biology (General) misc Chemistry
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title	Identification and Functional Characterization of Tissue-Specific Terpene Synthases in <i<Stevia rebaudiana</i<
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title_full	Identification and Functional Characterization of Tissue-Specific Terpene Synthases in <i<Stevia rebaudiana</i<
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title_sort	identification and functional characterization of tissue-specific terpene synthases in <i<stevia rebaudiana</i<
callnumber	QH301-705.5
title_auth	Identification and Functional Characterization of Tissue-Specific Terpene Synthases in <i<Stevia rebaudiana</i<
abstract	In addition to the well-known diterpenoid steviol glycosides, <i<Stevia rebaudiana</i< (Stevia) produces many labdane-type diterpenoids and a wide range of mono- and sesquiterpenoids. However, biosynthesis of mono- and sesquiterpenoids in Stevia remains unknown. Here we analyzed the extracts of Stevia leaves, flowers, stems, and roots by Gas Chromatography–Mass Spectrometry and putatively identified a total of 69 volatile organic compounds, most of which were terpenoids with considerably varied quantities among the four tissues of Stevia. Using Stevia transcriptomes, we identified and functionally characterized five terpene synthases (TPSs) that produced major mono- and sesquiterpenoids in Stevia. Transcript levels of these Stevia <i<TPS</i<s and levels of corresponding terpenoids correlated well in Stevia tissues. Particularly, the root-specific SrTPS4 and SrTPS5 catalyzed the formation of γ-curcumene/zingiberene/β-sesquiphellandrene and α-longipinene/β-himachalene/himachalol as multifunctional sesqui-TPSs, respectively. Most of the <i<SrTPSs</i< were highly responsive to various environmental stresses in a tissue-specific manner. Taken together, our results provide new insights into how Stevia produces diverse terpenoids to confer differential responses to various environmental factors in each tissue.
abstractGer	In addition to the well-known diterpenoid steviol glycosides, <i<Stevia rebaudiana</i< (Stevia) produces many labdane-type diterpenoids and a wide range of mono- and sesquiterpenoids. However, biosynthesis of mono- and sesquiterpenoids in Stevia remains unknown. Here we analyzed the extracts of Stevia leaves, flowers, stems, and roots by Gas Chromatography–Mass Spectrometry and putatively identified a total of 69 volatile organic compounds, most of which were terpenoids with considerably varied quantities among the four tissues of Stevia. Using Stevia transcriptomes, we identified and functionally characterized five terpene synthases (TPSs) that produced major mono- and sesquiterpenoids in Stevia. Transcript levels of these Stevia <i<TPS</i<s and levels of corresponding terpenoids correlated well in Stevia tissues. Particularly, the root-specific SrTPS4 and SrTPS5 catalyzed the formation of γ-curcumene/zingiberene/β-sesquiphellandrene and α-longipinene/β-himachalene/himachalol as multifunctional sesqui-TPSs, respectively. Most of the <i<SrTPSs</i< were highly responsive to various environmental stresses in a tissue-specific manner. Taken together, our results provide new insights into how Stevia produces diverse terpenoids to confer differential responses to various environmental factors in each tissue.
abstract_unstemmed	In addition to the well-known diterpenoid steviol glycosides, <i<Stevia rebaudiana</i< (Stevia) produces many labdane-type diterpenoids and a wide range of mono- and sesquiterpenoids. However, biosynthesis of mono- and sesquiterpenoids in Stevia remains unknown. Here we analyzed the extracts of Stevia leaves, flowers, stems, and roots by Gas Chromatography–Mass Spectrometry and putatively identified a total of 69 volatile organic compounds, most of which were terpenoids with considerably varied quantities among the four tissues of Stevia. Using Stevia transcriptomes, we identified and functionally characterized five terpene synthases (TPSs) that produced major mono- and sesquiterpenoids in Stevia. Transcript levels of these Stevia <i<TPS</i<s and levels of corresponding terpenoids correlated well in Stevia tissues. Particularly, the root-specific SrTPS4 and SrTPS5 catalyzed the formation of γ-curcumene/zingiberene/β-sesquiphellandrene and α-longipinene/β-himachalene/himachalol as multifunctional sesqui-TPSs, respectively. Most of the <i<SrTPSs</i< were highly responsive to various environmental stresses in a tissue-specific manner. Taken together, our results provide new insights into how Stevia produces diverse terpenoids to confer differential responses to various environmental factors in each tissue.
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container_issue	22, p 8566
title_short	Identification and Functional Characterization of Tissue-Specific Terpene Synthases in <i<Stevia rebaudiana</i<
url	https://doi.org/10.3390/ijms21228566 https://doaj.org/article/bba48014524a4918a65e2bc2a8daea83 https://www.mdpi.com/1422-0067/21/22/8566 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067
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Identification and Functional Characterization of Tissue-Specific Terpene Synthases in <i<Stevia rebaudiana</i<

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