Molecular Cloning and Expression Analysis of the ζ-Carotene Desaturase Gene in Chinese kale (Brassica oleracea var. alboglabra Bailey)

ζ-Carotene desaturase (ZDS) is an important enzyme in carotenoid biosynthesis. Here, the Brassica oleracea var. alboglabra ZDS (BoaZDS) gene was cloned from Chinese kale via reverse transcription-polymerase chain reaction (RT-PCR) and deposited in GenBank (accession number KY662297). The BoaZDS gene...
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Autor*in:	Bo SUN [verfasserIn] Fen ZHANG [verfasserIn] Shengling XUE [verfasserIn] Jiaqi CHANG [verfasserIn] Aihong ZHENG [verfasserIn] Min JIANG [verfasserIn] Huiying MIAO [verfasserIn] Qiaomei WANG [verfasserIn] Haoru TANG [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Übergeordnetes Werk:	In: Horticultural Plant Journal - KeAi Communications Co., Ltd., 2017, 4(2018), 3, Seite 94-102
Übergeordnetes Werk:	volume:4 ; year:2018 ; number:3 ; pages:94-102

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.hpj.2018.03.005

Katalog-ID:	DOAJ051943484

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520			\|a ζ-Carotene desaturase (ZDS) is an important enzyme in carotenoid biosynthesis. Here, the Brassica oleracea var. alboglabra ZDS (BoaZDS) gene was cloned from Chinese kale via reverse transcription-polymerase chain reaction (RT-PCR) and deposited in GenBank (accession number KY662297). The BoaZDS gene contains an open reading frame of 1 686 bp that encodes a 561-amino acid protein. Sequence analysis indicates that the ZDS protein is apparently conserved during plant evolution and is most closely related to B. oleracea var. capitata and B. rapa. The promoter sequence of the BoaZDS gene was predicted to harbor several cis-acting elements that are related to light and phytohormone responses. Semi-quantitative RT-PCR analysis showed that BoaZDS expression varied among different developmental stages and organs. Relative ZDS expression remained stable during germination and seedling stages and rapidly increased at the mature leaf stage. The leaves showed the highest ZDS expression levels compared to the other organs. ZDS expression decreased in all flower tissues during blooming. The fused protein of BoaZDS was obtained by prokaryotic expression. Heterologous expression of BoaZDS in Escherichia coli confirmed that BoaZDS encodes a functional ζ-carotene desaturase that increases β-carotene accumulation in E. coli cells harboring a β-carotene-producing plasmid. The findings of the present study provide a molecular basis for the elucidation of ZDS gene function in Chinese kale. Keywords: Chinese kale, Brassica oleracea var. alboglabra Bailey, carotenoids, ζ-carotene desaturase (ZDS), cloning, gene expression, prokaryotic expression, heterologous expression
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spelling	10.1016/j.hpj.2018.03.005 doi (DE-627)DOAJ051943484 (DE-599)DOAJ47872e10014f4656bbbcfad572eb06c9 DE-627 ger DE-627 rakwb eng SB1-1110 Bo SUN verfasserin aut Molecular Cloning and Expression Analysis of the ζ-Carotene Desaturase Gene in Chinese kale (Brassica oleracea var. alboglabra Bailey) 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ζ-Carotene desaturase (ZDS) is an important enzyme in carotenoid biosynthesis. Here, the Brassica oleracea var. alboglabra ZDS (BoaZDS) gene was cloned from Chinese kale via reverse transcription-polymerase chain reaction (RT-PCR) and deposited in GenBank (accession number KY662297). The BoaZDS gene contains an open reading frame of 1 686 bp that encodes a 561-amino acid protein. Sequence analysis indicates that the ZDS protein is apparently conserved during plant evolution and is most closely related to B. oleracea var. capitata and B. rapa. The promoter sequence of the BoaZDS gene was predicted to harbor several cis-acting elements that are related to light and phytohormone responses. Semi-quantitative RT-PCR analysis showed that BoaZDS expression varied among different developmental stages and organs. Relative ZDS expression remained stable during germination and seedling stages and rapidly increased at the mature leaf stage. The leaves showed the highest ZDS expression levels compared to the other organs. ZDS expression decreased in all flower tissues during blooming. The fused protein of BoaZDS was obtained by prokaryotic expression. Heterologous expression of BoaZDS in Escherichia coli confirmed that BoaZDS encodes a functional ζ-carotene desaturase that increases β-carotene accumulation in E. coli cells harboring a β-carotene-producing plasmid. The findings of the present study provide a molecular basis for the elucidation of ZDS gene function in Chinese kale. Keywords: Chinese kale, Brassica oleracea var. alboglabra Bailey, carotenoids, ζ-carotene desaturase (ZDS), cloning, gene expression, prokaryotic expression, heterologous expression Plant culture Fen ZHANG verfasserin aut Shengling XUE verfasserin aut Jiaqi CHANG verfasserin aut Aihong ZHENG verfasserin aut Min JIANG verfasserin aut Huiying MIAO verfasserin aut Qiaomei WANG verfasserin aut Haoru TANG verfasserin aut In Horticultural Plant Journal KeAi Communications Co., Ltd., 2017 4(2018), 3, Seite 94-102 (DE-627)880564024 (DE-600)2885064-6 24680141 nnns volume:4 year:2018 number:3 pages:94-102 https://doi.org/10.1016/j.hpj.2018.03.005 kostenfrei https://doaj.org/article/47872e10014f4656bbbcfad572eb06c9 kostenfrei http://www.sciencedirect.com/science/article/pii/S2468014118300608 kostenfrei https://doaj.org/toc/2468-0141 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 4 2018 3 94-102
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allfieldsGer	10.1016/j.hpj.2018.03.005 doi (DE-627)DOAJ051943484 (DE-599)DOAJ47872e10014f4656bbbcfad572eb06c9 DE-627 ger DE-627 rakwb eng SB1-1110 Bo SUN verfasserin aut Molecular Cloning and Expression Analysis of the ζ-Carotene Desaturase Gene in Chinese kale (Brassica oleracea var. alboglabra Bailey) 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ζ-Carotene desaturase (ZDS) is an important enzyme in carotenoid biosynthesis. Here, the Brassica oleracea var. alboglabra ZDS (BoaZDS) gene was cloned from Chinese kale via reverse transcription-polymerase chain reaction (RT-PCR) and deposited in GenBank (accession number KY662297). The BoaZDS gene contains an open reading frame of 1 686 bp that encodes a 561-amino acid protein. Sequence analysis indicates that the ZDS protein is apparently conserved during plant evolution and is most closely related to B. oleracea var. capitata and B. rapa. The promoter sequence of the BoaZDS gene was predicted to harbor several cis-acting elements that are related to light and phytohormone responses. Semi-quantitative RT-PCR analysis showed that BoaZDS expression varied among different developmental stages and organs. Relative ZDS expression remained stable during germination and seedling stages and rapidly increased at the mature leaf stage. The leaves showed the highest ZDS expression levels compared to the other organs. ZDS expression decreased in all flower tissues during blooming. The fused protein of BoaZDS was obtained by prokaryotic expression. Heterologous expression of BoaZDS in Escherichia coli confirmed that BoaZDS encodes a functional ζ-carotene desaturase that increases β-carotene accumulation in E. coli cells harboring a β-carotene-producing plasmid. The findings of the present study provide a molecular basis for the elucidation of ZDS gene function in Chinese kale. Keywords: Chinese kale, Brassica oleracea var. alboglabra Bailey, carotenoids, ζ-carotene desaturase (ZDS), cloning, gene expression, prokaryotic expression, heterologous expression Plant culture Fen ZHANG verfasserin aut Shengling XUE verfasserin aut Jiaqi CHANG verfasserin aut Aihong ZHENG verfasserin aut Min JIANG verfasserin aut Huiying MIAO verfasserin aut Qiaomei WANG verfasserin aut Haoru TANG verfasserin aut In Horticultural Plant Journal KeAi Communications Co., Ltd., 2017 4(2018), 3, Seite 94-102 (DE-627)880564024 (DE-600)2885064-6 24680141 nnns volume:4 year:2018 number:3 pages:94-102 https://doi.org/10.1016/j.hpj.2018.03.005 kostenfrei https://doaj.org/article/47872e10014f4656bbbcfad572eb06c9 kostenfrei http://www.sciencedirect.com/science/article/pii/S2468014118300608 kostenfrei https://doaj.org/toc/2468-0141 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 4 2018 3 94-102
allfieldsSound	10.1016/j.hpj.2018.03.005 doi (DE-627)DOAJ051943484 (DE-599)DOAJ47872e10014f4656bbbcfad572eb06c9 DE-627 ger DE-627 rakwb eng SB1-1110 Bo SUN verfasserin aut Molecular Cloning and Expression Analysis of the ζ-Carotene Desaturase Gene in Chinese kale (Brassica oleracea var. alboglabra Bailey) 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier ζ-Carotene desaturase (ZDS) is an important enzyme in carotenoid biosynthesis. Here, the Brassica oleracea var. alboglabra ZDS (BoaZDS) gene was cloned from Chinese kale via reverse transcription-polymerase chain reaction (RT-PCR) and deposited in GenBank (accession number KY662297). The BoaZDS gene contains an open reading frame of 1 686 bp that encodes a 561-amino acid protein. Sequence analysis indicates that the ZDS protein is apparently conserved during plant evolution and is most closely related to B. oleracea var. capitata and B. rapa. The promoter sequence of the BoaZDS gene was predicted to harbor several cis-acting elements that are related to light and phytohormone responses. Semi-quantitative RT-PCR analysis showed that BoaZDS expression varied among different developmental stages and organs. Relative ZDS expression remained stable during germination and seedling stages and rapidly increased at the mature leaf stage. The leaves showed the highest ZDS expression levels compared to the other organs. ZDS expression decreased in all flower tissues during blooming. The fused protein of BoaZDS was obtained by prokaryotic expression. Heterologous expression of BoaZDS in Escherichia coli confirmed that BoaZDS encodes a functional ζ-carotene desaturase that increases β-carotene accumulation in E. coli cells harboring a β-carotene-producing plasmid. The findings of the present study provide a molecular basis for the elucidation of ZDS gene function in Chinese kale. Keywords: Chinese kale, Brassica oleracea var. alboglabra Bailey, carotenoids, ζ-carotene desaturase (ZDS), cloning, gene expression, prokaryotic expression, heterologous expression Plant culture Fen ZHANG verfasserin aut Shengling XUE verfasserin aut Jiaqi CHANG verfasserin aut Aihong ZHENG verfasserin aut Min JIANG verfasserin aut Huiying MIAO verfasserin aut Qiaomei WANG verfasserin aut Haoru TANG verfasserin aut In Horticultural Plant Journal KeAi Communications Co., Ltd., 2017 4(2018), 3, Seite 94-102 (DE-627)880564024 (DE-600)2885064-6 24680141 nnns volume:4 year:2018 number:3 pages:94-102 https://doi.org/10.1016/j.hpj.2018.03.005 kostenfrei https://doaj.org/article/47872e10014f4656bbbcfad572eb06c9 kostenfrei http://www.sciencedirect.com/science/article/pii/S2468014118300608 kostenfrei https://doaj.org/toc/2468-0141 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4367 GBV_ILN_4700 AR 4 2018 3 94-102
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callnumber-first	S - Agriculture
author	Bo SUN
spellingShingle	Bo SUN misc SB1-1110 misc Plant culture Molecular Cloning and Expression Analysis of the ζ-Carotene Desaturase Gene in Chinese kale (Brassica oleracea var. alboglabra Bailey)
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topic_title	SB1-1110 Molecular Cloning and Expression Analysis of the ζ-Carotene Desaturase Gene in Chinese kale (Brassica oleracea var. alboglabra Bailey)
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title	Molecular Cloning and Expression Analysis of the ζ-Carotene Desaturase Gene in Chinese kale (Brassica oleracea var. alboglabra Bailey)
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title_full	Molecular Cloning and Expression Analysis of the ζ-Carotene Desaturase Gene in Chinese kale (Brassica oleracea var. alboglabra Bailey)
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author_browse	Bo SUN Fen ZHANG Shengling XUE Jiaqi CHANG Aihong ZHENG Min JIANG Huiying MIAO Qiaomei WANG Haoru TANG
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title_sort	molecular cloning and expression analysis of the ζ-carotene desaturase gene in chinese kale (brassica oleracea var. alboglabra bailey)
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title_auth	Molecular Cloning and Expression Analysis of the ζ-Carotene Desaturase Gene in Chinese kale (Brassica oleracea var. alboglabra Bailey)
abstract	ζ-Carotene desaturase (ZDS) is an important enzyme in carotenoid biosynthesis. Here, the Brassica oleracea var. alboglabra ZDS (BoaZDS) gene was cloned from Chinese kale via reverse transcription-polymerase chain reaction (RT-PCR) and deposited in GenBank (accession number KY662297). The BoaZDS gene contains an open reading frame of 1 686 bp that encodes a 561-amino acid protein. Sequence analysis indicates that the ZDS protein is apparently conserved during plant evolution and is most closely related to B. oleracea var. capitata and B. rapa. The promoter sequence of the BoaZDS gene was predicted to harbor several cis-acting elements that are related to light and phytohormone responses. Semi-quantitative RT-PCR analysis showed that BoaZDS expression varied among different developmental stages and organs. Relative ZDS expression remained stable during germination and seedling stages and rapidly increased at the mature leaf stage. The leaves showed the highest ZDS expression levels compared to the other organs. ZDS expression decreased in all flower tissues during blooming. The fused protein of BoaZDS was obtained by prokaryotic expression. Heterologous expression of BoaZDS in Escherichia coli confirmed that BoaZDS encodes a functional ζ-carotene desaturase that increases β-carotene accumulation in E. coli cells harboring a β-carotene-producing plasmid. The findings of the present study provide a molecular basis for the elucidation of ZDS gene function in Chinese kale. Keywords: Chinese kale, Brassica oleracea var. alboglabra Bailey, carotenoids, ζ-carotene desaturase (ZDS), cloning, gene expression, prokaryotic expression, heterologous expression
abstractGer	ζ-Carotene desaturase (ZDS) is an important enzyme in carotenoid biosynthesis. Here, the Brassica oleracea var. alboglabra ZDS (BoaZDS) gene was cloned from Chinese kale via reverse transcription-polymerase chain reaction (RT-PCR) and deposited in GenBank (accession number KY662297). The BoaZDS gene contains an open reading frame of 1 686 bp that encodes a 561-amino acid protein. Sequence analysis indicates that the ZDS protein is apparently conserved during plant evolution and is most closely related to B. oleracea var. capitata and B. rapa. The promoter sequence of the BoaZDS gene was predicted to harbor several cis-acting elements that are related to light and phytohormone responses. Semi-quantitative RT-PCR analysis showed that BoaZDS expression varied among different developmental stages and organs. Relative ZDS expression remained stable during germination and seedling stages and rapidly increased at the mature leaf stage. The leaves showed the highest ZDS expression levels compared to the other organs. ZDS expression decreased in all flower tissues during blooming. The fused protein of BoaZDS was obtained by prokaryotic expression. Heterologous expression of BoaZDS in Escherichia coli confirmed that BoaZDS encodes a functional ζ-carotene desaturase that increases β-carotene accumulation in E. coli cells harboring a β-carotene-producing plasmid. The findings of the present study provide a molecular basis for the elucidation of ZDS gene function in Chinese kale. Keywords: Chinese kale, Brassica oleracea var. alboglabra Bailey, carotenoids, ζ-carotene desaturase (ZDS), cloning, gene expression, prokaryotic expression, heterologous expression
abstract_unstemmed	ζ-Carotene desaturase (ZDS) is an important enzyme in carotenoid biosynthesis. Here, the Brassica oleracea var. alboglabra ZDS (BoaZDS) gene was cloned from Chinese kale via reverse transcription-polymerase chain reaction (RT-PCR) and deposited in GenBank (accession number KY662297). The BoaZDS gene contains an open reading frame of 1 686 bp that encodes a 561-amino acid protein. Sequence analysis indicates that the ZDS protein is apparently conserved during plant evolution and is most closely related to B. oleracea var. capitata and B. rapa. The promoter sequence of the BoaZDS gene was predicted to harbor several cis-acting elements that are related to light and phytohormone responses. Semi-quantitative RT-PCR analysis showed that BoaZDS expression varied among different developmental stages and organs. Relative ZDS expression remained stable during germination and seedling stages and rapidly increased at the mature leaf stage. The leaves showed the highest ZDS expression levels compared to the other organs. ZDS expression decreased in all flower tissues during blooming. The fused protein of BoaZDS was obtained by prokaryotic expression. Heterologous expression of BoaZDS in Escherichia coli confirmed that BoaZDS encodes a functional ζ-carotene desaturase that increases β-carotene accumulation in E. coli cells harboring a β-carotene-producing plasmid. The findings of the present study provide a molecular basis for the elucidation of ZDS gene function in Chinese kale. Keywords: Chinese kale, Brassica oleracea var. alboglabra Bailey, carotenoids, ζ-carotene desaturase (ZDS), cloning, gene expression, prokaryotic expression, heterologous expression
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title_short	Molecular Cloning and Expression Analysis of the ζ-Carotene Desaturase Gene in Chinese kale (Brassica oleracea var. alboglabra Bailey)
url	https://doi.org/10.1016/j.hpj.2018.03.005 https://doaj.org/article/47872e10014f4656bbbcfad572eb06c9 http://www.sciencedirect.com/science/article/pii/S2468014118300608 https://doaj.org/toc/2468-0141
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Here, the Brassica oleracea var. alboglabra ZDS (BoaZDS) gene was cloned from Chinese kale via reverse transcription-polymerase chain reaction (RT-PCR) and deposited in GenBank (accession number KY662297). The BoaZDS gene contains an open reading frame of 1 686 bp that encodes a 561-amino acid protein. Sequence analysis indicates that the ZDS protein is apparently conserved during plant evolution and is most closely related to B. oleracea var. capitata and B. rapa. The promoter sequence of the BoaZDS gene was predicted to harbor several cis-acting elements that are related to light and phytohormone responses. Semi-quantitative RT-PCR analysis showed that BoaZDS expression varied among different developmental stages and organs. Relative ZDS expression remained stable during germination and seedling stages and rapidly increased at the mature leaf stage. The leaves showed the highest ZDS expression levels compared to the other organs. ZDS expression decreased in all flower tissues during blooming. The fused protein of BoaZDS was obtained by prokaryotic expression. Heterologous expression of BoaZDS in Escherichia coli confirmed that BoaZDS encodes a functional ζ-carotene desaturase that increases β-carotene accumulation in E. coli cells harboring a β-carotene-producing plasmid. The findings of the present study provide a molecular basis for the elucidation of ZDS gene function in Chinese kale. 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Molecular Cloning and Expression Analysis of the ζ-Carotene Desaturase Gene in Chinese kale (Brassica oleracea var. alboglabra Bailey)

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