Immunization with Individual Proteins of the Lrp/AsnC Family Induces Protection Against Brucella melitensis 16M Challenges in Mice

Brucellosis is one of the most common zoonoses worldwide. Subunit vaccines are promising for the prevention of human brucellosis. In our previous protective antigen screening studies, we identified a new protective antigen, BMEI0357, which belongs to the Lrp/asnC protein family, a conserved transcri...
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Autor*in:	Xinhui eWang [verfasserIn] Chang eAn [verfasserIn] Mingjuan eYang [verfasserIn] Xinran eLi [verfasserIn] Yuehua eKe [verfasserIn] Shuangshuang eLei [verfasserIn] Xiaoyang eXu [verfasserIn] Jiuxuan eYu [verfasserIn] Hang eRen [verfasserIn] xinying eDu [verfasserIn] zhoujia ewang [verfasserIn] Yefeng eQiu [verfasserIn] Bo eLiu [verfasserIn] Zeliang eChen [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2015

Schlagwörter:	Brucella melitensis Brucellosis protective antigen Protective immune response Lrp/AsnC tran

Übergeordnetes Werk:	In: Frontiers in Microbiology - Frontiers Media S.A., 2011, 6(2015)
Übergeordnetes Werk:	volume:6 ; year:2015

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fmicb.2015.01193

Katalog-ID:	DOAJ052292673

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520			\|a Brucellosis is one of the most common zoonoses worldwide. Subunit vaccines are promising for the prevention of human brucellosis. In our previous protective antigen screening studies, we identified a new protective antigen, BMEI0357, which belongs to the Lrp/asnC protein family, a conserved transcriptional regulator in bacteria that is absent in eukaryotes. In the present study, the Brucella genome annotation was screened and a total of 6 proteins were identified as members of the Lrp/AsnC family. Lrp/AsnC proteins have 2 domains that are conserved among the family members. However, sequence similarities between these proteins ranged from 9% to 50%, indicating high sequence heterogeneity. To test whether proteins of this family have similar characteristics, all 6 proteins were cloned and expressed in Escherichia coli. The recombinant proteins were purified and their protective efficacy was evaluated in BALB/c mice challenged with Brucella melitensis 16M. The results show that all 6 Lrp/AsnC proteins could induce a protective immune response against Brucella melitensis 16M. Antibodies against the Lrp/AsnC proteins were detected in the immunized mice. However, levels of antibodies against these proteins were relatively variable in human brucellosis sera. Taken together, our results show that these 6 proteins of the Lrp/AsnC family in Brucella could induce protective immune responses in mice.
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author_variant	x e xe x e xe x e xe c e ce c e ce m e me x e xe x e xe y e ye s e se x e xe j e je h e he x e xe z e ze y e ye b e be z e ze
matchkey_str	article:1664302X:2015----::muiainihniiulrtisfhlpscaiynuepoetoaantrcla
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allfields	10.3389/fmicb.2015.01193 doi (DE-627)DOAJ052292673 (DE-599)DOAJ8dd1fa5ce44145d6847b266447c83587 DE-627 ger DE-627 rakwb eng QR1-502 Xinhui eWang verfasserin aut Immunization with Individual Proteins of the Lrp/AsnC Family Induces Protection Against Brucella melitensis 16M Challenges in Mice 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Brucellosis is one of the most common zoonoses worldwide. Subunit vaccines are promising for the prevention of human brucellosis. In our previous protective antigen screening studies, we identified a new protective antigen, BMEI0357, which belongs to the Lrp/asnC protein family, a conserved transcriptional regulator in bacteria that is absent in eukaryotes. In the present study, the Brucella genome annotation was screened and a total of 6 proteins were identified as members of the Lrp/AsnC family. Lrp/AsnC proteins have 2 domains that are conserved among the family members. However, sequence similarities between these proteins ranged from 9% to 50%, indicating high sequence heterogeneity. To test whether proteins of this family have similar characteristics, all 6 proteins were cloned and expressed in Escherichia coli. The recombinant proteins were purified and their protective efficacy was evaluated in BALB/c mice challenged with Brucella melitensis 16M. The results show that all 6 Lrp/AsnC proteins could induce a protective immune response against Brucella melitensis 16M. Antibodies against the Lrp/AsnC proteins were detected in the immunized mice. However, levels of antibodies against these proteins were relatively variable in human brucellosis sera. Taken together, our results show that these 6 proteins of the Lrp/AsnC family in Brucella could induce protective immune responses in mice. Brucella melitensis Brucellosis protective antigen Protective immune response Lrp/AsnC tran Microbiology Xinhui eWang verfasserin aut Xinhui eWang verfasserin aut Chang eAn verfasserin aut Chang eAn verfasserin aut Mingjuan eYang verfasserin aut Xinran eLi verfasserin aut Xinran eLi verfasserin aut Yuehua eKe verfasserin aut Shuangshuang eLei verfasserin aut Xiaoyang eXu verfasserin aut Jiuxuan eYu verfasserin aut Hang eRen verfasserin aut xinying eDu verfasserin aut zhoujia ewang verfasserin aut Yefeng eQiu verfasserin aut Bo eLiu verfasserin aut Zeliang eChen verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 6(2015) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:6 year:2015 https://doi.org/10.3389/fmicb.2015.01193 kostenfrei https://doaj.org/article/8dd1fa5ce44145d6847b266447c83587 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01193/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2015
spelling	10.3389/fmicb.2015.01193 doi (DE-627)DOAJ052292673 (DE-599)DOAJ8dd1fa5ce44145d6847b266447c83587 DE-627 ger DE-627 rakwb eng QR1-502 Xinhui eWang verfasserin aut Immunization with Individual Proteins of the Lrp/AsnC Family Induces Protection Against Brucella melitensis 16M Challenges in Mice 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Brucellosis is one of the most common zoonoses worldwide. Subunit vaccines are promising for the prevention of human brucellosis. In our previous protective antigen screening studies, we identified a new protective antigen, BMEI0357, which belongs to the Lrp/asnC protein family, a conserved transcriptional regulator in bacteria that is absent in eukaryotes. In the present study, the Brucella genome annotation was screened and a total of 6 proteins were identified as members of the Lrp/AsnC family. Lrp/AsnC proteins have 2 domains that are conserved among the family members. However, sequence similarities between these proteins ranged from 9% to 50%, indicating high sequence heterogeneity. To test whether proteins of this family have similar characteristics, all 6 proteins were cloned and expressed in Escherichia coli. The recombinant proteins were purified and their protective efficacy was evaluated in BALB/c mice challenged with Brucella melitensis 16M. The results show that all 6 Lrp/AsnC proteins could induce a protective immune response against Brucella melitensis 16M. Antibodies against the Lrp/AsnC proteins were detected in the immunized mice. However, levels of antibodies against these proteins were relatively variable in human brucellosis sera. Taken together, our results show that these 6 proteins of the Lrp/AsnC family in Brucella could induce protective immune responses in mice. Brucella melitensis Brucellosis protective antigen Protective immune response Lrp/AsnC tran Microbiology Xinhui eWang verfasserin aut Xinhui eWang verfasserin aut Chang eAn verfasserin aut Chang eAn verfasserin aut Mingjuan eYang verfasserin aut Xinran eLi verfasserin aut Xinran eLi verfasserin aut Yuehua eKe verfasserin aut Shuangshuang eLei verfasserin aut Xiaoyang eXu verfasserin aut Jiuxuan eYu verfasserin aut Hang eRen verfasserin aut xinying eDu verfasserin aut zhoujia ewang verfasserin aut Yefeng eQiu verfasserin aut Bo eLiu verfasserin aut Zeliang eChen verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 6(2015) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:6 year:2015 https://doi.org/10.3389/fmicb.2015.01193 kostenfrei https://doaj.org/article/8dd1fa5ce44145d6847b266447c83587 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01193/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2015
allfields_unstemmed	10.3389/fmicb.2015.01193 doi (DE-627)DOAJ052292673 (DE-599)DOAJ8dd1fa5ce44145d6847b266447c83587 DE-627 ger DE-627 rakwb eng QR1-502 Xinhui eWang verfasserin aut Immunization with Individual Proteins of the Lrp/AsnC Family Induces Protection Against Brucella melitensis 16M Challenges in Mice 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Brucellosis is one of the most common zoonoses worldwide. Subunit vaccines are promising for the prevention of human brucellosis. In our previous protective antigen screening studies, we identified a new protective antigen, BMEI0357, which belongs to the Lrp/asnC protein family, a conserved transcriptional regulator in bacteria that is absent in eukaryotes. In the present study, the Brucella genome annotation was screened and a total of 6 proteins were identified as members of the Lrp/AsnC family. Lrp/AsnC proteins have 2 domains that are conserved among the family members. However, sequence similarities between these proteins ranged from 9% to 50%, indicating high sequence heterogeneity. To test whether proteins of this family have similar characteristics, all 6 proteins were cloned and expressed in Escherichia coli. The recombinant proteins were purified and their protective efficacy was evaluated in BALB/c mice challenged with Brucella melitensis 16M. The results show that all 6 Lrp/AsnC proteins could induce a protective immune response against Brucella melitensis 16M. Antibodies against the Lrp/AsnC proteins were detected in the immunized mice. However, levels of antibodies against these proteins were relatively variable in human brucellosis sera. Taken together, our results show that these 6 proteins of the Lrp/AsnC family in Brucella could induce protective immune responses in mice. Brucella melitensis Brucellosis protective antigen Protective immune response Lrp/AsnC tran Microbiology Xinhui eWang verfasserin aut Xinhui eWang verfasserin aut Chang eAn verfasserin aut Chang eAn verfasserin aut Mingjuan eYang verfasserin aut Xinran eLi verfasserin aut Xinran eLi verfasserin aut Yuehua eKe verfasserin aut Shuangshuang eLei verfasserin aut Xiaoyang eXu verfasserin aut Jiuxuan eYu verfasserin aut Hang eRen verfasserin aut xinying eDu verfasserin aut zhoujia ewang verfasserin aut Yefeng eQiu verfasserin aut Bo eLiu verfasserin aut Zeliang eChen verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 6(2015) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:6 year:2015 https://doi.org/10.3389/fmicb.2015.01193 kostenfrei https://doaj.org/article/8dd1fa5ce44145d6847b266447c83587 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01193/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2015
allfieldsGer	10.3389/fmicb.2015.01193 doi (DE-627)DOAJ052292673 (DE-599)DOAJ8dd1fa5ce44145d6847b266447c83587 DE-627 ger DE-627 rakwb eng QR1-502 Xinhui eWang verfasserin aut Immunization with Individual Proteins of the Lrp/AsnC Family Induces Protection Against Brucella melitensis 16M Challenges in Mice 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Brucellosis is one of the most common zoonoses worldwide. Subunit vaccines are promising for the prevention of human brucellosis. In our previous protective antigen screening studies, we identified a new protective antigen, BMEI0357, which belongs to the Lrp/asnC protein family, a conserved transcriptional regulator in bacteria that is absent in eukaryotes. In the present study, the Brucella genome annotation was screened and a total of 6 proteins were identified as members of the Lrp/AsnC family. Lrp/AsnC proteins have 2 domains that are conserved among the family members. However, sequence similarities between these proteins ranged from 9% to 50%, indicating high sequence heterogeneity. To test whether proteins of this family have similar characteristics, all 6 proteins were cloned and expressed in Escherichia coli. The recombinant proteins were purified and their protective efficacy was evaluated in BALB/c mice challenged with Brucella melitensis 16M. The results show that all 6 Lrp/AsnC proteins could induce a protective immune response against Brucella melitensis 16M. Antibodies against the Lrp/AsnC proteins were detected in the immunized mice. However, levels of antibodies against these proteins were relatively variable in human brucellosis sera. Taken together, our results show that these 6 proteins of the Lrp/AsnC family in Brucella could induce protective immune responses in mice. Brucella melitensis Brucellosis protective antigen Protective immune response Lrp/AsnC tran Microbiology Xinhui eWang verfasserin aut Xinhui eWang verfasserin aut Chang eAn verfasserin aut Chang eAn verfasserin aut Mingjuan eYang verfasserin aut Xinran eLi verfasserin aut Xinran eLi verfasserin aut Yuehua eKe verfasserin aut Shuangshuang eLei verfasserin aut Xiaoyang eXu verfasserin aut Jiuxuan eYu verfasserin aut Hang eRen verfasserin aut xinying eDu verfasserin aut zhoujia ewang verfasserin aut Yefeng eQiu verfasserin aut Bo eLiu verfasserin aut Zeliang eChen verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 6(2015) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:6 year:2015 https://doi.org/10.3389/fmicb.2015.01193 kostenfrei https://doaj.org/article/8dd1fa5ce44145d6847b266447c83587 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01193/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2015
allfieldsSound	10.3389/fmicb.2015.01193 doi (DE-627)DOAJ052292673 (DE-599)DOAJ8dd1fa5ce44145d6847b266447c83587 DE-627 ger DE-627 rakwb eng QR1-502 Xinhui eWang verfasserin aut Immunization with Individual Proteins of the Lrp/AsnC Family Induces Protection Against Brucella melitensis 16M Challenges in Mice 2015 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Brucellosis is one of the most common zoonoses worldwide. Subunit vaccines are promising for the prevention of human brucellosis. In our previous protective antigen screening studies, we identified a new protective antigen, BMEI0357, which belongs to the Lrp/asnC protein family, a conserved transcriptional regulator in bacteria that is absent in eukaryotes. In the present study, the Brucella genome annotation was screened and a total of 6 proteins were identified as members of the Lrp/AsnC family. Lrp/AsnC proteins have 2 domains that are conserved among the family members. However, sequence similarities between these proteins ranged from 9% to 50%, indicating high sequence heterogeneity. To test whether proteins of this family have similar characteristics, all 6 proteins were cloned and expressed in Escherichia coli. The recombinant proteins were purified and their protective efficacy was evaluated in BALB/c mice challenged with Brucella melitensis 16M. The results show that all 6 Lrp/AsnC proteins could induce a protective immune response against Brucella melitensis 16M. Antibodies against the Lrp/AsnC proteins were detected in the immunized mice. However, levels of antibodies against these proteins were relatively variable in human brucellosis sera. Taken together, our results show that these 6 proteins of the Lrp/AsnC family in Brucella could induce protective immune responses in mice. Brucella melitensis Brucellosis protective antigen Protective immune response Lrp/AsnC tran Microbiology Xinhui eWang verfasserin aut Xinhui eWang verfasserin aut Chang eAn verfasserin aut Chang eAn verfasserin aut Mingjuan eYang verfasserin aut Xinran eLi verfasserin aut Xinran eLi verfasserin aut Yuehua eKe verfasserin aut Shuangshuang eLei verfasserin aut Xiaoyang eXu verfasserin aut Jiuxuan eYu verfasserin aut Hang eRen verfasserin aut xinying eDu verfasserin aut zhoujia ewang verfasserin aut Yefeng eQiu verfasserin aut Bo eLiu verfasserin aut Zeliang eChen verfasserin aut In Frontiers in Microbiology Frontiers Media S.A., 2011 6(2015) (DE-627)642889384 (DE-600)2587354-4 1664302X nnns volume:6 year:2015 https://doi.org/10.3389/fmicb.2015.01193 kostenfrei https://doaj.org/article/8dd1fa5ce44145d6847b266447c83587 kostenfrei http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01193/full kostenfrei https://doaj.org/toc/1664-302X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2015
language	English
source	In Frontiers in Microbiology 6(2015) volume:6 year:2015
sourceStr	In Frontiers in Microbiology 6(2015) volume:6 year:2015
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Brucella melitensis Brucellosis protective antigen Protective immune response Lrp/AsnC tran Microbiology
isfreeaccess_bool	true
container_title	Frontiers in Microbiology
authorswithroles_txt_mv	Xinhui eWang @@aut@@ Chang eAn @@aut@@ Mingjuan eYang @@aut@@ Xinran eLi @@aut@@ Yuehua eKe @@aut@@ Shuangshuang eLei @@aut@@ Xiaoyang eXu @@aut@@ Jiuxuan eYu @@aut@@ Hang eRen @@aut@@ xinying eDu @@aut@@ zhoujia ewang @@aut@@ Yefeng eQiu @@aut@@ Bo eLiu @@aut@@ Zeliang eChen @@aut@@
publishDateDaySort_date	2015-01-01T00:00:00Z
hierarchy_top_id	642889384
id	DOAJ052292673
language_de	englisch
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callnumber-first	Q - Science
author	Xinhui eWang
spellingShingle	Xinhui eWang misc QR1-502 misc Brucella melitensis misc Brucellosis misc protective antigen misc Protective immune response misc Lrp/AsnC tran misc Microbiology Immunization with Individual Proteins of the Lrp/AsnC Family Induces Protection Against Brucella melitensis 16M Challenges in Mice
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topic_title	QR1-502 Immunization with Individual Proteins of the Lrp/AsnC Family Induces Protection Against Brucella melitensis 16M Challenges in Mice Brucella melitensis Brucellosis protective antigen Protective immune response Lrp/AsnC tran
topic	misc QR1-502 misc Brucella melitensis misc Brucellosis misc protective antigen misc Protective immune response misc Lrp/AsnC tran misc Microbiology
topic_unstemmed	misc QR1-502 misc Brucella melitensis misc Brucellosis misc protective antigen misc Protective immune response misc Lrp/AsnC tran misc Microbiology
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title	Immunization with Individual Proteins of the Lrp/AsnC Family Induces Protection Against Brucella melitensis 16M Challenges in Mice
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title_full	Immunization with Individual Proteins of the Lrp/AsnC Family Induces Protection Against Brucella melitensis 16M Challenges in Mice
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journal	Frontiers in Microbiology
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author_browse	Xinhui eWang Chang eAn Mingjuan eYang Xinran eLi Yuehua eKe Shuangshuang eLei Xiaoyang eXu Jiuxuan eYu Hang eRen xinying eDu zhoujia ewang Yefeng eQiu Bo eLiu Zeliang eChen
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title_sort	immunization with individual proteins of the lrp/asnc family induces protection against brucella melitensis 16m challenges in mice
callnumber	QR1-502
title_auth	Immunization with Individual Proteins of the Lrp/AsnC Family Induces Protection Against Brucella melitensis 16M Challenges in Mice
abstract	Brucellosis is one of the most common zoonoses worldwide. Subunit vaccines are promising for the prevention of human brucellosis. In our previous protective antigen screening studies, we identified a new protective antigen, BMEI0357, which belongs to the Lrp/asnC protein family, a conserved transcriptional regulator in bacteria that is absent in eukaryotes. In the present study, the Brucella genome annotation was screened and a total of 6 proteins were identified as members of the Lrp/AsnC family. Lrp/AsnC proteins have 2 domains that are conserved among the family members. However, sequence similarities between these proteins ranged from 9% to 50%, indicating high sequence heterogeneity. To test whether proteins of this family have similar characteristics, all 6 proteins were cloned and expressed in Escherichia coli. The recombinant proteins were purified and their protective efficacy was evaluated in BALB/c mice challenged with Brucella melitensis 16M. The results show that all 6 Lrp/AsnC proteins could induce a protective immune response against Brucella melitensis 16M. Antibodies against the Lrp/AsnC proteins were detected in the immunized mice. However, levels of antibodies against these proteins were relatively variable in human brucellosis sera. Taken together, our results show that these 6 proteins of the Lrp/AsnC family in Brucella could induce protective immune responses in mice.
abstractGer	Brucellosis is one of the most common zoonoses worldwide. Subunit vaccines are promising for the prevention of human brucellosis. In our previous protective antigen screening studies, we identified a new protective antigen, BMEI0357, which belongs to the Lrp/asnC protein family, a conserved transcriptional regulator in bacteria that is absent in eukaryotes. In the present study, the Brucella genome annotation was screened and a total of 6 proteins were identified as members of the Lrp/AsnC family. Lrp/AsnC proteins have 2 domains that are conserved among the family members. However, sequence similarities between these proteins ranged from 9% to 50%, indicating high sequence heterogeneity. To test whether proteins of this family have similar characteristics, all 6 proteins were cloned and expressed in Escherichia coli. The recombinant proteins were purified and their protective efficacy was evaluated in BALB/c mice challenged with Brucella melitensis 16M. The results show that all 6 Lrp/AsnC proteins could induce a protective immune response against Brucella melitensis 16M. Antibodies against the Lrp/AsnC proteins were detected in the immunized mice. However, levels of antibodies against these proteins were relatively variable in human brucellosis sera. Taken together, our results show that these 6 proteins of the Lrp/AsnC family in Brucella could induce protective immune responses in mice.
abstract_unstemmed	Brucellosis is one of the most common zoonoses worldwide. Subunit vaccines are promising for the prevention of human brucellosis. In our previous protective antigen screening studies, we identified a new protective antigen, BMEI0357, which belongs to the Lrp/asnC protein family, a conserved transcriptional regulator in bacteria that is absent in eukaryotes. In the present study, the Brucella genome annotation was screened and a total of 6 proteins were identified as members of the Lrp/AsnC family. Lrp/AsnC proteins have 2 domains that are conserved among the family members. However, sequence similarities between these proteins ranged from 9% to 50%, indicating high sequence heterogeneity. To test whether proteins of this family have similar characteristics, all 6 proteins were cloned and expressed in Escherichia coli. The recombinant proteins were purified and their protective efficacy was evaluated in BALB/c mice challenged with Brucella melitensis 16M. The results show that all 6 Lrp/AsnC proteins could induce a protective immune response against Brucella melitensis 16M. Antibodies against the Lrp/AsnC proteins were detected in the immunized mice. However, levels of antibodies against these proteins were relatively variable in human brucellosis sera. Taken together, our results show that these 6 proteins of the Lrp/AsnC family in Brucella could induce protective immune responses in mice.
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title_short	Immunization with Individual Proteins of the Lrp/AsnC Family Induces Protection Against Brucella melitensis 16M Challenges in Mice
url	https://doi.org/10.3389/fmicb.2015.01193 https://doaj.org/article/8dd1fa5ce44145d6847b266447c83587 http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01193/full https://doaj.org/toc/1664-302X
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author2	Xinhui eWang Chang eAn Mingjuan eYang Xinran eLi Yuehua eKe Shuangshuang eLei Xiaoyang eXu Jiuxuan eYu Hang eRen xinying eDu zhoujia ewang Yefeng eQiu Bo eLiu Zeliang eChen
author2Str	Xinhui eWang Chang eAn Mingjuan eYang Xinran eLi Yuehua eKe Shuangshuang eLei Xiaoyang eXu Jiuxuan eYu Hang eRen xinying eDu zhoujia ewang Yefeng eQiu Bo eLiu Zeliang eChen
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up_date	2024-07-04T00:30:02.227Z
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Immunization with Individual Proteins of the Lrp/AsnC Family Induces Protection Against Brucella melitensis 16M Challenges in Mice

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