Cryopreservation of Hazelnut (<i<Corylus avellana</i< L.) Axillary Buds from <i<In Vitro</i< Shoots Using the Droplet Vitrification Method

Cryopreservation by droplet vitrification was applied to hazelnut (<i<Corylus avellana</i< L.). axillary buds of the Italian cultivated variety Tonda Gentile Romana, which were collected from <i<in vitro</i< growing shoots, immersed in ice cooled PVS2 or PVS3 for 60 or 90 min...
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Gespeichert in:

Autor*in:	Alessandra Sgueglia [verfasserIn] Andrea Frattarelli [verfasserIn] Adele Gentile [verfasserIn] Gaia Urbinati [verfasserIn] Simona Lucioli [verfasserIn] Maria Antonietta Germanà [verfasserIn] Emilia Caboni [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	length of dehydration PVS2 PVS3 regrowth rooting

Übergeordnetes Werk:	In: Horticulturae - MDPI AG, 2017, 7(2021), 11, p 494
Übergeordnetes Werk:	volume:7 ; year:2021 ; number:11, p 494

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/horticulturae7110494

Katalog-ID:	DOAJ052391078

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authorswithroles_txt_mv	Alessandra Sgueglia @@aut@@ Andrea Frattarelli @@aut@@ Adele Gentile @@aut@@ Gaia Urbinati @@aut@@ Simona Lucioli @@aut@@ Maria Antonietta Germanà @@aut@@ Emilia Caboni @@aut@@
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callnumber-first	S - Agriculture
author	Alessandra Sgueglia
spellingShingle	Alessandra Sgueglia misc SB1-1110 misc <i<in vitro</i< culture misc length of dehydration misc PVS2 misc PVS3 misc regrowth misc rooting misc Plant culture Cryopreservation of Hazelnut (<i<Corylus avellana</i< L.) Axillary Buds from <i<In Vitro</i< Shoots Using the Droplet Vitrification Method
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topic_title	SB1-1110 Cryopreservation of Hazelnut (<i<Corylus avellana</i< L.) Axillary Buds from <i<In Vitro</i< Shoots Using the Droplet Vitrification Method <i<in vitro</i< culture length of dehydration PVS2 PVS3 regrowth rooting
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title	Cryopreservation of Hazelnut (<i<Corylus avellana</i< L.) Axillary Buds from <i<In Vitro</i< Shoots Using the Droplet Vitrification Method
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title_full	Cryopreservation of Hazelnut (<i<Corylus avellana</i< L.) Axillary Buds from <i<In Vitro</i< Shoots Using the Droplet Vitrification Method
author_sort	Alessandra Sgueglia
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title_sort	cryopreservation of hazelnut (<i<corylus avellana</i< l.) axillary buds from <i<in vitro</i< shoots using the droplet vitrification method
callnumber	SB1-1110
title_auth	Cryopreservation of Hazelnut (<i<Corylus avellana</i< L.) Axillary Buds from <i<In Vitro</i< Shoots Using the Droplet Vitrification Method
abstract	Cryopreservation by droplet vitrification was applied to hazelnut (<i<Corylus avellana</i< L.). axillary buds of the Italian cultivated variety Tonda Gentile Romana, which were collected from <i<in vitro</i< growing shoots, immersed in ice cooled PVS2 or PVS3 for 60 or 90 min, then transferred to a droplet of vitrification solution, placed on a strip of aluminium foil, and plunged into liquid nitrogen (LN). Additionally, the effect on the recovery of the mother plant after cryopreservation was evaluated, following a cold pre-treatment at 4 °C for 3 months. The highest regrowth percentage (56.7%) was obtained after applying PVS3 for 60 min, while the application of PVS2 for the same amount of time reduced regrowth to 41.5%. Increasing the exposure to vitrification solutions to 90 min reduced regrowth to 43.3% when PVS3 was applied, and 35.6% if PVS2 was used. The cold pre-treatment on the mother plant did not significantly improve overall regrowth. The cryopreservation process did not decline the rooting ability of the recovered shoots.
abstractGer	Cryopreservation by droplet vitrification was applied to hazelnut (<i<Corylus avellana</i< L.). axillary buds of the Italian cultivated variety Tonda Gentile Romana, which were collected from <i<in vitro</i< growing shoots, immersed in ice cooled PVS2 or PVS3 for 60 or 90 min, then transferred to a droplet of vitrification solution, placed on a strip of aluminium foil, and plunged into liquid nitrogen (LN). Additionally, the effect on the recovery of the mother plant after cryopreservation was evaluated, following a cold pre-treatment at 4 °C for 3 months. The highest regrowth percentage (56.7%) was obtained after applying PVS3 for 60 min, while the application of PVS2 for the same amount of time reduced regrowth to 41.5%. Increasing the exposure to vitrification solutions to 90 min reduced regrowth to 43.3% when PVS3 was applied, and 35.6% if PVS2 was used. The cold pre-treatment on the mother plant did not significantly improve overall regrowth. The cryopreservation process did not decline the rooting ability of the recovered shoots.
abstract_unstemmed	Cryopreservation by droplet vitrification was applied to hazelnut (<i<Corylus avellana</i< L.). axillary buds of the Italian cultivated variety Tonda Gentile Romana, which were collected from <i<in vitro</i< growing shoots, immersed in ice cooled PVS2 or PVS3 for 60 or 90 min, then transferred to a droplet of vitrification solution, placed on a strip of aluminium foil, and plunged into liquid nitrogen (LN). Additionally, the effect on the recovery of the mother plant after cryopreservation was evaluated, following a cold pre-treatment at 4 °C for 3 months. The highest regrowth percentage (56.7%) was obtained after applying PVS3 for 60 min, while the application of PVS2 for the same amount of time reduced regrowth to 41.5%. Increasing the exposure to vitrification solutions to 90 min reduced regrowth to 43.3% when PVS3 was applied, and 35.6% if PVS2 was used. The cold pre-treatment on the mother plant did not significantly improve overall regrowth. The cryopreservation process did not decline the rooting ability of the recovered shoots.
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container_issue	11, p 494
title_short	Cryopreservation of Hazelnut (<i<Corylus avellana</i< L.) Axillary Buds from <i<In Vitro</i< Shoots Using the Droplet Vitrification Method
url	https://doi.org/10.3390/horticulturae7110494 https://doaj.org/article/350b3eb3b04248bba32a900662003198 https://www.mdpi.com/2311-7524/7/11/494 https://doaj.org/toc/2311-7524
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Cryopreservation of Hazelnut (<i<Corylus avellana</i< L.) Axillary Buds from <i<In Vitro</i< Shoots Using the Droplet Vitrification Method

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