Purple corn extract (PCE) alleviates cigarette smoke (CS)-induced DNA damage in rodent blood cells by activation of AMPK/Foxo3a/MnSOD pathway

Purple corn extract (PCE) is a nutraceutical, an activator of AMPK, and it has antioxidants and anticancer properties. Therefore, PCE could be a candidate for alleviating cigarette smoke (CS)-induced oxidative DNA damage. This study examined whether PCE can have a protective effect on blood cells in...
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Autor*in:	Wan-Sik Kim [verfasserIn] Chea-Ha Kim [verfasserIn] Jung-Min Lee [verfasserIn] Jeong-Ho Jeon [verfasserIn] Beom-Goo Kang [verfasserIn] Madhuri Shende Warkad [verfasserIn] Gozde Inci [verfasserIn] Hong-Won Suh [verfasserIn] Soon Sung Lim [verfasserIn] Sung-Chan Kim [verfasserIn] Jaebong Kim [verfasserIn] Jae-Yong Lee [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	purple corn extract cigarette smoke dna damage blood cells ampkfoxo3a-mnsod

Übergeordnetes Werk:	In: Animal Cells and Systems - Taylor & Francis Group, 2018, 25(2021), 1, Seite 65-73
Übergeordnetes Werk:	volume:25 ; year:2021 ; number:1 ; pages:65-73

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.1080/19768354.2021.1883734

Katalog-ID:	DOAJ052492753

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520			\|a Purple corn extract (PCE) is a nutraceutical, an activator of AMPK, and it has antioxidants and anticancer properties. Therefore, PCE could be a candidate for alleviating cigarette smoke (CS)-induced oxidative DNA damage. This study examined whether PCE can have a protective effect on blood cells in an animal model of cigarette smoke (CS)-induced DNA damage. PCE was orally administered to CS-inhaled Spraque-Dawley (SD) rats, followed by the target cells being examined for markers of DNA damage. The study also sought to elucidate the mechanism of PCE action in the PCE treated animals. SD rat inhalation of CS was for once a day for 30 min, repeated for 7 days. PCE was administered orally before CS inhalation. Pretreatment of the animals with oral PCE kept the numbers of white blood cells (WBC) as well as neutrophils (NE), lymphocytes (LY), monocytes (Mo), eosinophils (EO), abd jasophils (BA) from increasing as those were increased in the CS-inhaling SD rats. The amount of phosphorylated γ-H2AX, a DNA damage marker, was assayed in the circulating blood cells collected from the animals and western blot analysis with anti-Foxo3a, p-Foxo3a, p-AMPK, MnSOD antibodies were performed on those cells. PCE protected the circulating blood cells from CS inhalation-induced DNA damage by 44% as assayed by increases in γ-H2AX. PCE also increased the nuclear localization of Foxo3a by 52% over control cells. Mechanistically, PCE appears to efficiently protect various blood cell types from CS-induced DNA damage through removal of ROS via activation of the AMPK/Foxo3a/MnSOD pathway.
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allfields	10.1080/19768354.2021.1883734 doi (DE-627)DOAJ052492753 (DE-599)DOAJ0cfdcc137f5640f28d7534476f5f7a89 DE-627 ger DE-627 rakwb eng R5-920 QH301-705.5 Wan-Sik Kim verfasserin aut Purple corn extract (PCE) alleviates cigarette smoke (CS)-induced DNA damage in rodent blood cells by activation of AMPK/Foxo3a/MnSOD pathway 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Purple corn extract (PCE) is a nutraceutical, an activator of AMPK, and it has antioxidants and anticancer properties. Therefore, PCE could be a candidate for alleviating cigarette smoke (CS)-induced oxidative DNA damage. This study examined whether PCE can have a protective effect on blood cells in an animal model of cigarette smoke (CS)-induced DNA damage. PCE was orally administered to CS-inhaled Spraque-Dawley (SD) rats, followed by the target cells being examined for markers of DNA damage. The study also sought to elucidate the mechanism of PCE action in the PCE treated animals. SD rat inhalation of CS was for once a day for 30 min, repeated for 7 days. PCE was administered orally before CS inhalation. Pretreatment of the animals with oral PCE kept the numbers of white blood cells (WBC) as well as neutrophils (NE), lymphocytes (LY), monocytes (Mo), eosinophils (EO), abd jasophils (BA) from increasing as those were increased in the CS-inhaling SD rats. The amount of phosphorylated γ-H2AX, a DNA damage marker, was assayed in the circulating blood cells collected from the animals and western blot analysis with anti-Foxo3a, p-Foxo3a, p-AMPK, MnSOD antibodies were performed on those cells. PCE protected the circulating blood cells from CS inhalation-induced DNA damage by 44% as assayed by increases in γ-H2AX. PCE also increased the nuclear localization of Foxo3a by 52% over control cells. Mechanistically, PCE appears to efficiently protect various blood cell types from CS-induced DNA damage through removal of ROS via activation of the AMPK/Foxo3a/MnSOD pathway. purple corn extract cigarette smoke dna damage blood cells ampkfoxo3a-mnsod Medicine (General) Biology (General) Chea-Ha Kim verfasserin aut Jung-Min Lee verfasserin aut Jeong-Ho Jeon verfasserin aut Beom-Goo Kang verfasserin aut Madhuri Shende Warkad verfasserin aut Gozde Inci verfasserin aut Hong-Won Suh verfasserin aut Soon Sung Lim verfasserin aut Sung-Chan Kim verfasserin aut Jaebong Kim verfasserin aut Jae-Yong Lee verfasserin aut In Animal Cells and Systems Taylor & Francis Group, 2018 25(2021), 1, Seite 65-73 (DE-627)631150064 (DE-600)2562988-8 21512485 nnns volume:25 year:2021 number:1 pages:65-73 https://doi.org/10.1080/19768354.2021.1883734 kostenfrei https://doaj.org/article/0cfdcc137f5640f28d7534476f5f7a89 kostenfrei http://dx.doi.org/10.1080/19768354.2021.1883734 kostenfrei https://doaj.org/toc/1976-8354 Journal toc kostenfrei https://doaj.org/toc/2151-2485 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2021 1 65-73
spelling	10.1080/19768354.2021.1883734 doi (DE-627)DOAJ052492753 (DE-599)DOAJ0cfdcc137f5640f28d7534476f5f7a89 DE-627 ger DE-627 rakwb eng R5-920 QH301-705.5 Wan-Sik Kim verfasserin aut Purple corn extract (PCE) alleviates cigarette smoke (CS)-induced DNA damage in rodent blood cells by activation of AMPK/Foxo3a/MnSOD pathway 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Purple corn extract (PCE) is a nutraceutical, an activator of AMPK, and it has antioxidants and anticancer properties. Therefore, PCE could be a candidate for alleviating cigarette smoke (CS)-induced oxidative DNA damage. This study examined whether PCE can have a protective effect on blood cells in an animal model of cigarette smoke (CS)-induced DNA damage. PCE was orally administered to CS-inhaled Spraque-Dawley (SD) rats, followed by the target cells being examined for markers of DNA damage. The study also sought to elucidate the mechanism of PCE action in the PCE treated animals. SD rat inhalation of CS was for once a day for 30 min, repeated for 7 days. PCE was administered orally before CS inhalation. Pretreatment of the animals with oral PCE kept the numbers of white blood cells (WBC) as well as neutrophils (NE), lymphocytes (LY), monocytes (Mo), eosinophils (EO), abd jasophils (BA) from increasing as those were increased in the CS-inhaling SD rats. The amount of phosphorylated γ-H2AX, a DNA damage marker, was assayed in the circulating blood cells collected from the animals and western blot analysis with anti-Foxo3a, p-Foxo3a, p-AMPK, MnSOD antibodies were performed on those cells. PCE protected the circulating blood cells from CS inhalation-induced DNA damage by 44% as assayed by increases in γ-H2AX. PCE also increased the nuclear localization of Foxo3a by 52% over control cells. Mechanistically, PCE appears to efficiently protect various blood cell types from CS-induced DNA damage through removal of ROS via activation of the AMPK/Foxo3a/MnSOD pathway. purple corn extract cigarette smoke dna damage blood cells ampkfoxo3a-mnsod Medicine (General) Biology (General) Chea-Ha Kim verfasserin aut Jung-Min Lee verfasserin aut Jeong-Ho Jeon verfasserin aut Beom-Goo Kang verfasserin aut Madhuri Shende Warkad verfasserin aut Gozde Inci verfasserin aut Hong-Won Suh verfasserin aut Soon Sung Lim verfasserin aut Sung-Chan Kim verfasserin aut Jaebong Kim verfasserin aut Jae-Yong Lee verfasserin aut In Animal Cells and Systems Taylor & Francis Group, 2018 25(2021), 1, Seite 65-73 (DE-627)631150064 (DE-600)2562988-8 21512485 nnns volume:25 year:2021 number:1 pages:65-73 https://doi.org/10.1080/19768354.2021.1883734 kostenfrei https://doaj.org/article/0cfdcc137f5640f28d7534476f5f7a89 kostenfrei http://dx.doi.org/10.1080/19768354.2021.1883734 kostenfrei https://doaj.org/toc/1976-8354 Journal toc kostenfrei https://doaj.org/toc/2151-2485 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2021 1 65-73
allfields_unstemmed	10.1080/19768354.2021.1883734 doi (DE-627)DOAJ052492753 (DE-599)DOAJ0cfdcc137f5640f28d7534476f5f7a89 DE-627 ger DE-627 rakwb eng R5-920 QH301-705.5 Wan-Sik Kim verfasserin aut Purple corn extract (PCE) alleviates cigarette smoke (CS)-induced DNA damage in rodent blood cells by activation of AMPK/Foxo3a/MnSOD pathway 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Purple corn extract (PCE) is a nutraceutical, an activator of AMPK, and it has antioxidants and anticancer properties. Therefore, PCE could be a candidate for alleviating cigarette smoke (CS)-induced oxidative DNA damage. This study examined whether PCE can have a protective effect on blood cells in an animal model of cigarette smoke (CS)-induced DNA damage. PCE was orally administered to CS-inhaled Spraque-Dawley (SD) rats, followed by the target cells being examined for markers of DNA damage. The study also sought to elucidate the mechanism of PCE action in the PCE treated animals. SD rat inhalation of CS was for once a day for 30 min, repeated for 7 days. PCE was administered orally before CS inhalation. Pretreatment of the animals with oral PCE kept the numbers of white blood cells (WBC) as well as neutrophils (NE), lymphocytes (LY), monocytes (Mo), eosinophils (EO), abd jasophils (BA) from increasing as those were increased in the CS-inhaling SD rats. The amount of phosphorylated γ-H2AX, a DNA damage marker, was assayed in the circulating blood cells collected from the animals and western blot analysis with anti-Foxo3a, p-Foxo3a, p-AMPK, MnSOD antibodies were performed on those cells. PCE protected the circulating blood cells from CS inhalation-induced DNA damage by 44% as assayed by increases in γ-H2AX. PCE also increased the nuclear localization of Foxo3a by 52% over control cells. Mechanistically, PCE appears to efficiently protect various blood cell types from CS-induced DNA damage through removal of ROS via activation of the AMPK/Foxo3a/MnSOD pathway. purple corn extract cigarette smoke dna damage blood cells ampkfoxo3a-mnsod Medicine (General) Biology (General) Chea-Ha Kim verfasserin aut Jung-Min Lee verfasserin aut Jeong-Ho Jeon verfasserin aut Beom-Goo Kang verfasserin aut Madhuri Shende Warkad verfasserin aut Gozde Inci verfasserin aut Hong-Won Suh verfasserin aut Soon Sung Lim verfasserin aut Sung-Chan Kim verfasserin aut Jaebong Kim verfasserin aut Jae-Yong Lee verfasserin aut In Animal Cells and Systems Taylor & Francis Group, 2018 25(2021), 1, Seite 65-73 (DE-627)631150064 (DE-600)2562988-8 21512485 nnns volume:25 year:2021 number:1 pages:65-73 https://doi.org/10.1080/19768354.2021.1883734 kostenfrei https://doaj.org/article/0cfdcc137f5640f28d7534476f5f7a89 kostenfrei http://dx.doi.org/10.1080/19768354.2021.1883734 kostenfrei https://doaj.org/toc/1976-8354 Journal toc kostenfrei https://doaj.org/toc/2151-2485 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2021 1 65-73
allfieldsGer	10.1080/19768354.2021.1883734 doi (DE-627)DOAJ052492753 (DE-599)DOAJ0cfdcc137f5640f28d7534476f5f7a89 DE-627 ger DE-627 rakwb eng R5-920 QH301-705.5 Wan-Sik Kim verfasserin aut Purple corn extract (PCE) alleviates cigarette smoke (CS)-induced DNA damage in rodent blood cells by activation of AMPK/Foxo3a/MnSOD pathway 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Purple corn extract (PCE) is a nutraceutical, an activator of AMPK, and it has antioxidants and anticancer properties. Therefore, PCE could be a candidate for alleviating cigarette smoke (CS)-induced oxidative DNA damage. This study examined whether PCE can have a protective effect on blood cells in an animal model of cigarette smoke (CS)-induced DNA damage. PCE was orally administered to CS-inhaled Spraque-Dawley (SD) rats, followed by the target cells being examined for markers of DNA damage. The study also sought to elucidate the mechanism of PCE action in the PCE treated animals. SD rat inhalation of CS was for once a day for 30 min, repeated for 7 days. PCE was administered orally before CS inhalation. Pretreatment of the animals with oral PCE kept the numbers of white blood cells (WBC) as well as neutrophils (NE), lymphocytes (LY), monocytes (Mo), eosinophils (EO), abd jasophils (BA) from increasing as those were increased in the CS-inhaling SD rats. The amount of phosphorylated γ-H2AX, a DNA damage marker, was assayed in the circulating blood cells collected from the animals and western blot analysis with anti-Foxo3a, p-Foxo3a, p-AMPK, MnSOD antibodies were performed on those cells. PCE protected the circulating blood cells from CS inhalation-induced DNA damage by 44% as assayed by increases in γ-H2AX. PCE also increased the nuclear localization of Foxo3a by 52% over control cells. Mechanistically, PCE appears to efficiently protect various blood cell types from CS-induced DNA damage through removal of ROS via activation of the AMPK/Foxo3a/MnSOD pathway. purple corn extract cigarette smoke dna damage blood cells ampkfoxo3a-mnsod Medicine (General) Biology (General) Chea-Ha Kim verfasserin aut Jung-Min Lee verfasserin aut Jeong-Ho Jeon verfasserin aut Beom-Goo Kang verfasserin aut Madhuri Shende Warkad verfasserin aut Gozde Inci verfasserin aut Hong-Won Suh verfasserin aut Soon Sung Lim verfasserin aut Sung-Chan Kim verfasserin aut Jaebong Kim verfasserin aut Jae-Yong Lee verfasserin aut In Animal Cells and Systems Taylor & Francis Group, 2018 25(2021), 1, Seite 65-73 (DE-627)631150064 (DE-600)2562988-8 21512485 nnns volume:25 year:2021 number:1 pages:65-73 https://doi.org/10.1080/19768354.2021.1883734 kostenfrei https://doaj.org/article/0cfdcc137f5640f28d7534476f5f7a89 kostenfrei http://dx.doi.org/10.1080/19768354.2021.1883734 kostenfrei https://doaj.org/toc/1976-8354 Journal toc kostenfrei https://doaj.org/toc/2151-2485 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2021 1 65-73
allfieldsSound	10.1080/19768354.2021.1883734 doi (DE-627)DOAJ052492753 (DE-599)DOAJ0cfdcc137f5640f28d7534476f5f7a89 DE-627 ger DE-627 rakwb eng R5-920 QH301-705.5 Wan-Sik Kim verfasserin aut Purple corn extract (PCE) alleviates cigarette smoke (CS)-induced DNA damage in rodent blood cells by activation of AMPK/Foxo3a/MnSOD pathway 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Purple corn extract (PCE) is a nutraceutical, an activator of AMPK, and it has antioxidants and anticancer properties. Therefore, PCE could be a candidate for alleviating cigarette smoke (CS)-induced oxidative DNA damage. This study examined whether PCE can have a protective effect on blood cells in an animal model of cigarette smoke (CS)-induced DNA damage. PCE was orally administered to CS-inhaled Spraque-Dawley (SD) rats, followed by the target cells being examined for markers of DNA damage. The study also sought to elucidate the mechanism of PCE action in the PCE treated animals. SD rat inhalation of CS was for once a day for 30 min, repeated for 7 days. PCE was administered orally before CS inhalation. Pretreatment of the animals with oral PCE kept the numbers of white blood cells (WBC) as well as neutrophils (NE), lymphocytes (LY), monocytes (Mo), eosinophils (EO), abd jasophils (BA) from increasing as those were increased in the CS-inhaling SD rats. The amount of phosphorylated γ-H2AX, a DNA damage marker, was assayed in the circulating blood cells collected from the animals and western blot analysis with anti-Foxo3a, p-Foxo3a, p-AMPK, MnSOD antibodies were performed on those cells. PCE protected the circulating blood cells from CS inhalation-induced DNA damage by 44% as assayed by increases in γ-H2AX. PCE also increased the nuclear localization of Foxo3a by 52% over control cells. Mechanistically, PCE appears to efficiently protect various blood cell types from CS-induced DNA damage through removal of ROS via activation of the AMPK/Foxo3a/MnSOD pathway. purple corn extract cigarette smoke dna damage blood cells ampkfoxo3a-mnsod Medicine (General) Biology (General) Chea-Ha Kim verfasserin aut Jung-Min Lee verfasserin aut Jeong-Ho Jeon verfasserin aut Beom-Goo Kang verfasserin aut Madhuri Shende Warkad verfasserin aut Gozde Inci verfasserin aut Hong-Won Suh verfasserin aut Soon Sung Lim verfasserin aut Sung-Chan Kim verfasserin aut Jaebong Kim verfasserin aut Jae-Yong Lee verfasserin aut In Animal Cells and Systems Taylor & Francis Group, 2018 25(2021), 1, Seite 65-73 (DE-627)631150064 (DE-600)2562988-8 21512485 nnns volume:25 year:2021 number:1 pages:65-73 https://doi.org/10.1080/19768354.2021.1883734 kostenfrei https://doaj.org/article/0cfdcc137f5640f28d7534476f5f7a89 kostenfrei http://dx.doi.org/10.1080/19768354.2021.1883734 kostenfrei https://doaj.org/toc/1976-8354 Journal toc kostenfrei https://doaj.org/toc/2151-2485 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 25 2021 1 65-73
language	English
source	In Animal Cells and Systems 25(2021), 1, Seite 65-73 volume:25 year:2021 number:1 pages:65-73
sourceStr	In Animal Cells and Systems 25(2021), 1, Seite 65-73 volume:25 year:2021 number:1 pages:65-73
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	purple corn extract cigarette smoke dna damage blood cells ampkfoxo3a-mnsod Medicine (General) Biology (General)
isfreeaccess_bool	true
container_title	Animal Cells and Systems
authorswithroles_txt_mv	Wan-Sik Kim @@aut@@ Chea-Ha Kim @@aut@@ Jung-Min Lee @@aut@@ Jeong-Ho Jeon @@aut@@ Beom-Goo Kang @@aut@@ Madhuri Shende Warkad @@aut@@ Gozde Inci @@aut@@ Hong-Won Suh @@aut@@ Soon Sung Lim @@aut@@ Sung-Chan Kim @@aut@@ Jaebong Kim @@aut@@ Jae-Yong Lee @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	631150064
id	DOAJ052492753
language_de	englisch
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callnumber-first	R - Medicine
author	Wan-Sik Kim
spellingShingle	Wan-Sik Kim misc R5-920 misc QH301-705.5 misc purple corn extract misc cigarette smoke misc dna damage misc blood cells misc ampkfoxo3a-mnsod misc Medicine (General) misc Biology (General) Purple corn extract (PCE) alleviates cigarette smoke (CS)-induced DNA damage in rodent blood cells by activation of AMPK/Foxo3a/MnSOD pathway
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topic_title	R5-920 QH301-705.5 Purple corn extract (PCE) alleviates cigarette smoke (CS)-induced DNA damage in rodent blood cells by activation of AMPK/Foxo3a/MnSOD pathway purple corn extract cigarette smoke dna damage blood cells ampkfoxo3a-mnsod
topic	misc R5-920 misc QH301-705.5 misc purple corn extract misc cigarette smoke misc dna damage misc blood cells misc ampkfoxo3a-mnsod misc Medicine (General) misc Biology (General)
topic_unstemmed	misc R5-920 misc QH301-705.5 misc purple corn extract misc cigarette smoke misc dna damage misc blood cells misc ampkfoxo3a-mnsod misc Medicine (General) misc Biology (General)
topic_browse	misc R5-920 misc QH301-705.5 misc purple corn extract misc cigarette smoke misc dna damage misc blood cells misc ampkfoxo3a-mnsod misc Medicine (General) misc Biology (General)
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title	Purple corn extract (PCE) alleviates cigarette smoke (CS)-induced DNA damage in rodent blood cells by activation of AMPK/Foxo3a/MnSOD pathway
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title_full	Purple corn extract (PCE) alleviates cigarette smoke (CS)-induced DNA damage in rodent blood cells by activation of AMPK/Foxo3a/MnSOD pathway
author_sort	Wan-Sik Kim
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author_browse	Wan-Sik Kim Chea-Ha Kim Jung-Min Lee Jeong-Ho Jeon Beom-Goo Kang Madhuri Shende Warkad Gozde Inci Hong-Won Suh Soon Sung Lim Sung-Chan Kim Jaebong Kim Jae-Yong Lee
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title_sort	purple corn extract (pce) alleviates cigarette smoke (cs)-induced dna damage in rodent blood cells by activation of ampk/foxo3a/mnsod pathway
callnumber	R5-920
title_auth	Purple corn extract (PCE) alleviates cigarette smoke (CS)-induced DNA damage in rodent blood cells by activation of AMPK/Foxo3a/MnSOD pathway
abstract	Purple corn extract (PCE) is a nutraceutical, an activator of AMPK, and it has antioxidants and anticancer properties. Therefore, PCE could be a candidate for alleviating cigarette smoke (CS)-induced oxidative DNA damage. This study examined whether PCE can have a protective effect on blood cells in an animal model of cigarette smoke (CS)-induced DNA damage. PCE was orally administered to CS-inhaled Spraque-Dawley (SD) rats, followed by the target cells being examined for markers of DNA damage. The study also sought to elucidate the mechanism of PCE action in the PCE treated animals. SD rat inhalation of CS was for once a day for 30 min, repeated for 7 days. PCE was administered orally before CS inhalation. Pretreatment of the animals with oral PCE kept the numbers of white blood cells (WBC) as well as neutrophils (NE), lymphocytes (LY), monocytes (Mo), eosinophils (EO), abd jasophils (BA) from increasing as those were increased in the CS-inhaling SD rats. The amount of phosphorylated γ-H2AX, a DNA damage marker, was assayed in the circulating blood cells collected from the animals and western blot analysis with anti-Foxo3a, p-Foxo3a, p-AMPK, MnSOD antibodies were performed on those cells. PCE protected the circulating blood cells from CS inhalation-induced DNA damage by 44% as assayed by increases in γ-H2AX. PCE also increased the nuclear localization of Foxo3a by 52% over control cells. Mechanistically, PCE appears to efficiently protect various blood cell types from CS-induced DNA damage through removal of ROS via activation of the AMPK/Foxo3a/MnSOD pathway.
abstractGer	Purple corn extract (PCE) is a nutraceutical, an activator of AMPK, and it has antioxidants and anticancer properties. Therefore, PCE could be a candidate for alleviating cigarette smoke (CS)-induced oxidative DNA damage. This study examined whether PCE can have a protective effect on blood cells in an animal model of cigarette smoke (CS)-induced DNA damage. PCE was orally administered to CS-inhaled Spraque-Dawley (SD) rats, followed by the target cells being examined for markers of DNA damage. The study also sought to elucidate the mechanism of PCE action in the PCE treated animals. SD rat inhalation of CS was for once a day for 30 min, repeated for 7 days. PCE was administered orally before CS inhalation. Pretreatment of the animals with oral PCE kept the numbers of white blood cells (WBC) as well as neutrophils (NE), lymphocytes (LY), monocytes (Mo), eosinophils (EO), abd jasophils (BA) from increasing as those were increased in the CS-inhaling SD rats. The amount of phosphorylated γ-H2AX, a DNA damage marker, was assayed in the circulating blood cells collected from the animals and western blot analysis with anti-Foxo3a, p-Foxo3a, p-AMPK, MnSOD antibodies were performed on those cells. PCE protected the circulating blood cells from CS inhalation-induced DNA damage by 44% as assayed by increases in γ-H2AX. PCE also increased the nuclear localization of Foxo3a by 52% over control cells. Mechanistically, PCE appears to efficiently protect various blood cell types from CS-induced DNA damage through removal of ROS via activation of the AMPK/Foxo3a/MnSOD pathway.
abstract_unstemmed	Purple corn extract (PCE) is a nutraceutical, an activator of AMPK, and it has antioxidants and anticancer properties. Therefore, PCE could be a candidate for alleviating cigarette smoke (CS)-induced oxidative DNA damage. This study examined whether PCE can have a protective effect on blood cells in an animal model of cigarette smoke (CS)-induced DNA damage. PCE was orally administered to CS-inhaled Spraque-Dawley (SD) rats, followed by the target cells being examined for markers of DNA damage. The study also sought to elucidate the mechanism of PCE action in the PCE treated animals. SD rat inhalation of CS was for once a day for 30 min, repeated for 7 days. PCE was administered orally before CS inhalation. Pretreatment of the animals with oral PCE kept the numbers of white blood cells (WBC) as well as neutrophils (NE), lymphocytes (LY), monocytes (Mo), eosinophils (EO), abd jasophils (BA) from increasing as those were increased in the CS-inhaling SD rats. The amount of phosphorylated γ-H2AX, a DNA damage marker, was assayed in the circulating blood cells collected from the animals and western blot analysis with anti-Foxo3a, p-Foxo3a, p-AMPK, MnSOD antibodies were performed on those cells. PCE protected the circulating blood cells from CS inhalation-induced DNA damage by 44% as assayed by increases in γ-H2AX. PCE also increased the nuclear localization of Foxo3a by 52% over control cells. Mechanistically, PCE appears to efficiently protect various blood cell types from CS-induced DNA damage through removal of ROS via activation of the AMPK/Foxo3a/MnSOD pathway.
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title_short	Purple corn extract (PCE) alleviates cigarette smoke (CS)-induced DNA damage in rodent blood cells by activation of AMPK/Foxo3a/MnSOD pathway
url	https://doi.org/10.1080/19768354.2021.1883734 https://doaj.org/article/0cfdcc137f5640f28d7534476f5f7a89 http://dx.doi.org/10.1080/19768354.2021.1883734 https://doaj.org/toc/1976-8354 https://doaj.org/toc/2151-2485
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author2Str	Chea-Ha Kim Jung-Min Lee Jeong-Ho Jeon Beom-Goo Kang Madhuri Shende Warkad Gozde Inci Hong-Won Suh Soon Sung Lim Sung-Chan Kim Jaebong Kim Jae-Yong Lee
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Purple corn extract (PCE) alleviates cigarette smoke (CS)-induced DNA damage in rodent blood cells by activation of AMPK/Foxo3a/MnSOD pathway

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