Biocatalytic synthesis of lactosucrose using a recombinant thermostable β-fructofuranosidase from Arthrobacter sp. 10138

As a prebiotics, lactosucrose plays an important role in maintaining human gastrointestinal homeostasis. In this study, a thermostable enzyme from Arthrobacter sp. 10138 was screened from six β-fructofuranosidase-producing strains for the lactosucrose production and the coding gene was heterologousl...
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Autor*in:	Chunmei Chen [verfasserIn] Jieying Deng [verfasserIn] Xueqin Lv [verfasserIn] Jianghua Li [verfasserIn] Guocheng Du [verfasserIn] Huazhong Li [verfasserIn] Long Liu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	lactosucrose thermostable β-fructofuranosidase arthrobacter sp. 10138 transfructosylation

Übergeordnetes Werk:	In: Bioengineered - Taylor & Francis Group, 2019, 11(2020), 1, Seite 416-427
Übergeordnetes Werk:	volume:11 ; year:2020 ; number:1 ; pages:416-427

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.1080/21655979.2020.1739404

Katalog-ID:	DOAJ05271294X

Internformat


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Indexfelder

author_variant	c c cc j d jd x l xl j l jl g d gd h l hl l l ll
matchkey_str	article:21655987:2020----::ictltcyteiolcoursuigrcmiathrotbercouaoi
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allfields	10.1080/21655979.2020.1739404 doi (DE-627)DOAJ05271294X (DE-599)DOAJa48d3899894b4ed9878747966c605478 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Chunmei Chen verfasserin aut Biocatalytic synthesis of lactosucrose using a recombinant thermostable β-fructofuranosidase from Arthrobacter sp. 10138 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As a prebiotics, lactosucrose plays an important role in maintaining human gastrointestinal homeostasis. In this study, a thermostable enzyme from Arthrobacter sp. 10138 was screened from six β-fructofuranosidase-producing strains for the lactosucrose production and the coding gene was heterologously expressed in Escherichia coli for efficient expression. Recombinant β-fructofuranosidase was purified and biochemically characterized by MALDI-TOFMS spectrometry. The transfructosylation product by this recombinant enzyme was determined to be lactosucrose rather than other oligosaccharides or polysaccharides by HPLC and LC-MS. Efficient extracellular secretion of β-fructofuranosidase was achieved by the optimization of signal peptide and induction conditions. It was found that with the signal peptide torT, the highest extracellular activity reached 111.01 U/mL, which was 38.4-fold higher than that with the OmpA signal peptide. Under the optimal conditions (pH 6.0, temperature 50°C, enzyme amount 40 μg/ml, sucrose 150 g/L and lactose 150 g/L), 109 g/L lactosucrose was produced with a molar conversion ratio of 49.3%. Here the thermostable β-fructofuranosidase from Arthrobacter sp. 10138 can be used for efficient synthesis of lactosucrose, and this provides a good startpoint for the industrial production of lactosucrose in the future. lactosucrose thermostable β-fructofuranosidase arthrobacter sp. 10138 transfructosylation Biotechnology Jieying Deng verfasserin aut Xueqin Lv verfasserin aut Jianghua Li verfasserin aut Guocheng Du verfasserin aut Huazhong Li verfasserin aut Long Liu verfasserin aut In Bioengineered Taylor & Francis Group, 2019 11(2020), 1, Seite 416-427 (DE-627)770398189 (DE-600)2737830-5 21655987 nnns volume:11 year:2020 number:1 pages:416-427 https://doi.org/10.1080/21655979.2020.1739404 kostenfrei https://doaj.org/article/a48d3899894b4ed9878747966c605478 kostenfrei http://dx.doi.org/10.1080/21655979.2020.1739404 kostenfrei https://doaj.org/toc/2165-5979 Journal toc kostenfrei https://doaj.org/toc/2165-5987 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2020 1 416-427
spelling	10.1080/21655979.2020.1739404 doi (DE-627)DOAJ05271294X (DE-599)DOAJa48d3899894b4ed9878747966c605478 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Chunmei Chen verfasserin aut Biocatalytic synthesis of lactosucrose using a recombinant thermostable β-fructofuranosidase from Arthrobacter sp. 10138 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As a prebiotics, lactosucrose plays an important role in maintaining human gastrointestinal homeostasis. In this study, a thermostable enzyme from Arthrobacter sp. 10138 was screened from six β-fructofuranosidase-producing strains for the lactosucrose production and the coding gene was heterologously expressed in Escherichia coli for efficient expression. Recombinant β-fructofuranosidase was purified and biochemically characterized by MALDI-TOFMS spectrometry. The transfructosylation product by this recombinant enzyme was determined to be lactosucrose rather than other oligosaccharides or polysaccharides by HPLC and LC-MS. Efficient extracellular secretion of β-fructofuranosidase was achieved by the optimization of signal peptide and induction conditions. It was found that with the signal peptide torT, the highest extracellular activity reached 111.01 U/mL, which was 38.4-fold higher than that with the OmpA signal peptide. Under the optimal conditions (pH 6.0, temperature 50°C, enzyme amount 40 μg/ml, sucrose 150 g/L and lactose 150 g/L), 109 g/L lactosucrose was produced with a molar conversion ratio of 49.3%. Here the thermostable β-fructofuranosidase from Arthrobacter sp. 10138 can be used for efficient synthesis of lactosucrose, and this provides a good startpoint for the industrial production of lactosucrose in the future. lactosucrose thermostable β-fructofuranosidase arthrobacter sp. 10138 transfructosylation Biotechnology Jieying Deng verfasserin aut Xueqin Lv verfasserin aut Jianghua Li verfasserin aut Guocheng Du verfasserin aut Huazhong Li verfasserin aut Long Liu verfasserin aut In Bioengineered Taylor & Francis Group, 2019 11(2020), 1, Seite 416-427 (DE-627)770398189 (DE-600)2737830-5 21655987 nnns volume:11 year:2020 number:1 pages:416-427 https://doi.org/10.1080/21655979.2020.1739404 kostenfrei https://doaj.org/article/a48d3899894b4ed9878747966c605478 kostenfrei http://dx.doi.org/10.1080/21655979.2020.1739404 kostenfrei https://doaj.org/toc/2165-5979 Journal toc kostenfrei https://doaj.org/toc/2165-5987 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2020 1 416-427
allfields_unstemmed	10.1080/21655979.2020.1739404 doi (DE-627)DOAJ05271294X (DE-599)DOAJa48d3899894b4ed9878747966c605478 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Chunmei Chen verfasserin aut Biocatalytic synthesis of lactosucrose using a recombinant thermostable β-fructofuranosidase from Arthrobacter sp. 10138 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As a prebiotics, lactosucrose plays an important role in maintaining human gastrointestinal homeostasis. In this study, a thermostable enzyme from Arthrobacter sp. 10138 was screened from six β-fructofuranosidase-producing strains for the lactosucrose production and the coding gene was heterologously expressed in Escherichia coli for efficient expression. Recombinant β-fructofuranosidase was purified and biochemically characterized by MALDI-TOFMS spectrometry. The transfructosylation product by this recombinant enzyme was determined to be lactosucrose rather than other oligosaccharides or polysaccharides by HPLC and LC-MS. Efficient extracellular secretion of β-fructofuranosidase was achieved by the optimization of signal peptide and induction conditions. It was found that with the signal peptide torT, the highest extracellular activity reached 111.01 U/mL, which was 38.4-fold higher than that with the OmpA signal peptide. Under the optimal conditions (pH 6.0, temperature 50°C, enzyme amount 40 μg/ml, sucrose 150 g/L and lactose 150 g/L), 109 g/L lactosucrose was produced with a molar conversion ratio of 49.3%. Here the thermostable β-fructofuranosidase from Arthrobacter sp. 10138 can be used for efficient synthesis of lactosucrose, and this provides a good startpoint for the industrial production of lactosucrose in the future. lactosucrose thermostable β-fructofuranosidase arthrobacter sp. 10138 transfructosylation Biotechnology Jieying Deng verfasserin aut Xueqin Lv verfasserin aut Jianghua Li verfasserin aut Guocheng Du verfasserin aut Huazhong Li verfasserin aut Long Liu verfasserin aut In Bioengineered Taylor & Francis Group, 2019 11(2020), 1, Seite 416-427 (DE-627)770398189 (DE-600)2737830-5 21655987 nnns volume:11 year:2020 number:1 pages:416-427 https://doi.org/10.1080/21655979.2020.1739404 kostenfrei https://doaj.org/article/a48d3899894b4ed9878747966c605478 kostenfrei http://dx.doi.org/10.1080/21655979.2020.1739404 kostenfrei https://doaj.org/toc/2165-5979 Journal toc kostenfrei https://doaj.org/toc/2165-5987 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2020 1 416-427
allfieldsGer	10.1080/21655979.2020.1739404 doi (DE-627)DOAJ05271294X (DE-599)DOAJa48d3899894b4ed9878747966c605478 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Chunmei Chen verfasserin aut Biocatalytic synthesis of lactosucrose using a recombinant thermostable β-fructofuranosidase from Arthrobacter sp. 10138 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As a prebiotics, lactosucrose plays an important role in maintaining human gastrointestinal homeostasis. In this study, a thermostable enzyme from Arthrobacter sp. 10138 was screened from six β-fructofuranosidase-producing strains for the lactosucrose production and the coding gene was heterologously expressed in Escherichia coli for efficient expression. Recombinant β-fructofuranosidase was purified and biochemically characterized by MALDI-TOFMS spectrometry. The transfructosylation product by this recombinant enzyme was determined to be lactosucrose rather than other oligosaccharides or polysaccharides by HPLC and LC-MS. Efficient extracellular secretion of β-fructofuranosidase was achieved by the optimization of signal peptide and induction conditions. It was found that with the signal peptide torT, the highest extracellular activity reached 111.01 U/mL, which was 38.4-fold higher than that with the OmpA signal peptide. Under the optimal conditions (pH 6.0, temperature 50°C, enzyme amount 40 μg/ml, sucrose 150 g/L and lactose 150 g/L), 109 g/L lactosucrose was produced with a molar conversion ratio of 49.3%. Here the thermostable β-fructofuranosidase from Arthrobacter sp. 10138 can be used for efficient synthesis of lactosucrose, and this provides a good startpoint for the industrial production of lactosucrose in the future. lactosucrose thermostable β-fructofuranosidase arthrobacter sp. 10138 transfructosylation Biotechnology Jieying Deng verfasserin aut Xueqin Lv verfasserin aut Jianghua Li verfasserin aut Guocheng Du verfasserin aut Huazhong Li verfasserin aut Long Liu verfasserin aut In Bioengineered Taylor & Francis Group, 2019 11(2020), 1, Seite 416-427 (DE-627)770398189 (DE-600)2737830-5 21655987 nnns volume:11 year:2020 number:1 pages:416-427 https://doi.org/10.1080/21655979.2020.1739404 kostenfrei https://doaj.org/article/a48d3899894b4ed9878747966c605478 kostenfrei http://dx.doi.org/10.1080/21655979.2020.1739404 kostenfrei https://doaj.org/toc/2165-5979 Journal toc kostenfrei https://doaj.org/toc/2165-5987 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2020 1 416-427
allfieldsSound	10.1080/21655979.2020.1739404 doi (DE-627)DOAJ05271294X (DE-599)DOAJa48d3899894b4ed9878747966c605478 DE-627 ger DE-627 rakwb eng TP248.13-248.65 Chunmei Chen verfasserin aut Biocatalytic synthesis of lactosucrose using a recombinant thermostable β-fructofuranosidase from Arthrobacter sp. 10138 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As a prebiotics, lactosucrose plays an important role in maintaining human gastrointestinal homeostasis. In this study, a thermostable enzyme from Arthrobacter sp. 10138 was screened from six β-fructofuranosidase-producing strains for the lactosucrose production and the coding gene was heterologously expressed in Escherichia coli for efficient expression. Recombinant β-fructofuranosidase was purified and biochemically characterized by MALDI-TOFMS spectrometry. The transfructosylation product by this recombinant enzyme was determined to be lactosucrose rather than other oligosaccharides or polysaccharides by HPLC and LC-MS. Efficient extracellular secretion of β-fructofuranosidase was achieved by the optimization of signal peptide and induction conditions. It was found that with the signal peptide torT, the highest extracellular activity reached 111.01 U/mL, which was 38.4-fold higher than that with the OmpA signal peptide. Under the optimal conditions (pH 6.0, temperature 50°C, enzyme amount 40 μg/ml, sucrose 150 g/L and lactose 150 g/L), 109 g/L lactosucrose was produced with a molar conversion ratio of 49.3%. Here the thermostable β-fructofuranosidase from Arthrobacter sp. 10138 can be used for efficient synthesis of lactosucrose, and this provides a good startpoint for the industrial production of lactosucrose in the future. lactosucrose thermostable β-fructofuranosidase arthrobacter sp. 10138 transfructosylation Biotechnology Jieying Deng verfasserin aut Xueqin Lv verfasserin aut Jianghua Li verfasserin aut Guocheng Du verfasserin aut Huazhong Li verfasserin aut Long Liu verfasserin aut In Bioengineered Taylor & Francis Group, 2019 11(2020), 1, Seite 416-427 (DE-627)770398189 (DE-600)2737830-5 21655987 nnns volume:11 year:2020 number:1 pages:416-427 https://doi.org/10.1080/21655979.2020.1739404 kostenfrei https://doaj.org/article/a48d3899894b4ed9878747966c605478 kostenfrei http://dx.doi.org/10.1080/21655979.2020.1739404 kostenfrei https://doaj.org/toc/2165-5979 Journal toc kostenfrei https://doaj.org/toc/2165-5987 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2020 1 416-427
language	English
source	In Bioengineered 11(2020), 1, Seite 416-427 volume:11 year:2020 number:1 pages:416-427
sourceStr	In Bioengineered 11(2020), 1, Seite 416-427 volume:11 year:2020 number:1 pages:416-427
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topic_facet	lactosucrose thermostable β-fructofuranosidase arthrobacter sp. 10138 transfructosylation Biotechnology
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authorswithroles_txt_mv	Chunmei Chen @@aut@@ Jieying Deng @@aut@@ Xueqin Lv @@aut@@ Jianghua Li @@aut@@ Guocheng Du @@aut@@ Huazhong Li @@aut@@ Long Liu @@aut@@
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callnumber-first	T - Technology
author	Chunmei Chen
spellingShingle	Chunmei Chen misc TP248.13-248.65 misc lactosucrose misc thermostable misc β-fructofuranosidase misc arthrobacter sp. 10138 misc transfructosylation misc Biotechnology Biocatalytic synthesis of lactosucrose using a recombinant thermostable β-fructofuranosidase from Arthrobacter sp. 10138
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topic_title	TP248.13-248.65 Biocatalytic synthesis of lactosucrose using a recombinant thermostable β-fructofuranosidase from Arthrobacter sp. 10138 lactosucrose thermostable β-fructofuranosidase arthrobacter sp. 10138 transfructosylation
topic	misc TP248.13-248.65 misc lactosucrose misc thermostable misc β-fructofuranosidase misc arthrobacter sp. 10138 misc transfructosylation misc Biotechnology
topic_unstemmed	misc TP248.13-248.65 misc lactosucrose misc thermostable misc β-fructofuranosidase misc arthrobacter sp. 10138 misc transfructosylation misc Biotechnology
topic_browse	misc TP248.13-248.65 misc lactosucrose misc thermostable misc β-fructofuranosidase misc arthrobacter sp. 10138 misc transfructosylation misc Biotechnology
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title	Biocatalytic synthesis of lactosucrose using a recombinant thermostable β-fructofuranosidase from Arthrobacter sp. 10138
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title_full	Biocatalytic synthesis of lactosucrose using a recombinant thermostable β-fructofuranosidase from Arthrobacter sp. 10138
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author_browse	Chunmei Chen Jieying Deng Xueqin Lv Jianghua Li Guocheng Du Huazhong Li Long Liu
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title_sort	biocatalytic synthesis of lactosucrose using a recombinant thermostable β-fructofuranosidase from arthrobacter sp. 10138
callnumber	TP248.13-248.65
title_auth	Biocatalytic synthesis of lactosucrose using a recombinant thermostable β-fructofuranosidase from Arthrobacter sp. 10138
abstract	As a prebiotics, lactosucrose plays an important role in maintaining human gastrointestinal homeostasis. In this study, a thermostable enzyme from Arthrobacter sp. 10138 was screened from six β-fructofuranosidase-producing strains for the lactosucrose production and the coding gene was heterologously expressed in Escherichia coli for efficient expression. Recombinant β-fructofuranosidase was purified and biochemically characterized by MALDI-TOFMS spectrometry. The transfructosylation product by this recombinant enzyme was determined to be lactosucrose rather than other oligosaccharides or polysaccharides by HPLC and LC-MS. Efficient extracellular secretion of β-fructofuranosidase was achieved by the optimization of signal peptide and induction conditions. It was found that with the signal peptide torT, the highest extracellular activity reached 111.01 U/mL, which was 38.4-fold higher than that with the OmpA signal peptide. Under the optimal conditions (pH 6.0, temperature 50°C, enzyme amount 40 μg/ml, sucrose 150 g/L and lactose 150 g/L), 109 g/L lactosucrose was produced with a molar conversion ratio of 49.3%. Here the thermostable β-fructofuranosidase from Arthrobacter sp. 10138 can be used for efficient synthesis of lactosucrose, and this provides a good startpoint for the industrial production of lactosucrose in the future.
abstractGer	As a prebiotics, lactosucrose plays an important role in maintaining human gastrointestinal homeostasis. In this study, a thermostable enzyme from Arthrobacter sp. 10138 was screened from six β-fructofuranosidase-producing strains for the lactosucrose production and the coding gene was heterologously expressed in Escherichia coli for efficient expression. Recombinant β-fructofuranosidase was purified and biochemically characterized by MALDI-TOFMS spectrometry. The transfructosylation product by this recombinant enzyme was determined to be lactosucrose rather than other oligosaccharides or polysaccharides by HPLC and LC-MS. Efficient extracellular secretion of β-fructofuranosidase was achieved by the optimization of signal peptide and induction conditions. It was found that with the signal peptide torT, the highest extracellular activity reached 111.01 U/mL, which was 38.4-fold higher than that with the OmpA signal peptide. Under the optimal conditions (pH 6.0, temperature 50°C, enzyme amount 40 μg/ml, sucrose 150 g/L and lactose 150 g/L), 109 g/L lactosucrose was produced with a molar conversion ratio of 49.3%. Here the thermostable β-fructofuranosidase from Arthrobacter sp. 10138 can be used for efficient synthesis of lactosucrose, and this provides a good startpoint for the industrial production of lactosucrose in the future.
abstract_unstemmed	As a prebiotics, lactosucrose plays an important role in maintaining human gastrointestinal homeostasis. In this study, a thermostable enzyme from Arthrobacter sp. 10138 was screened from six β-fructofuranosidase-producing strains for the lactosucrose production and the coding gene was heterologously expressed in Escherichia coli for efficient expression. Recombinant β-fructofuranosidase was purified and biochemically characterized by MALDI-TOFMS spectrometry. The transfructosylation product by this recombinant enzyme was determined to be lactosucrose rather than other oligosaccharides or polysaccharides by HPLC and LC-MS. Efficient extracellular secretion of β-fructofuranosidase was achieved by the optimization of signal peptide and induction conditions. It was found that with the signal peptide torT, the highest extracellular activity reached 111.01 U/mL, which was 38.4-fold higher than that with the OmpA signal peptide. Under the optimal conditions (pH 6.0, temperature 50°C, enzyme amount 40 μg/ml, sucrose 150 g/L and lactose 150 g/L), 109 g/L lactosucrose was produced with a molar conversion ratio of 49.3%. Here the thermostable β-fructofuranosidase from Arthrobacter sp. 10138 can be used for efficient synthesis of lactosucrose, and this provides a good startpoint for the industrial production of lactosucrose in the future.
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title_short	Biocatalytic synthesis of lactosucrose using a recombinant thermostable β-fructofuranosidase from Arthrobacter sp. 10138
url	https://doi.org/10.1080/21655979.2020.1739404 https://doaj.org/article/a48d3899894b4ed9878747966c605478 http://dx.doi.org/10.1080/21655979.2020.1739404 https://doaj.org/toc/2165-5979 https://doaj.org/toc/2165-5987
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Under the optimal conditions (pH 6.0, temperature 50°C, enzyme amount 40 μg/ml, sucrose 150 g/L and lactose 150 g/L), 109 g/L lactosucrose was produced with a molar conversion ratio of 49.3%. 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Biocatalytic synthesis of lactosucrose using a recombinant thermostable β-fructofuranosidase from Arthrobacter sp. 10138

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