Chemical analyses and in vitro and in vivo toxicity of fruit methanol extract of Sechium edule var. nigrum spinosum

Context: Sechium edule (Jacq.) Sw. (Cucurbitaceae) is used in ethnomedicine, but the diversity of the varietal groups of this species has not often been considered. This is important because we previously reported that different variety of species exhibit different activities across different tumor...
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Autor*in:	Itzen Aguiñiga-Sánchez [verfasserIn] Jorge Cadena-Íñiguez [verfasserIn] Edelmiro Santiago-Osorio [verfasserIn] Guadalupe Gómez-García [verfasserIn] Víctor Manuel Mendoza-Núñez [verfasserIn] Juana Rosado-Pérez [verfasserIn] Mirna Ruíz-Ramos [verfasserIn] Víctor Manuel Cisneros-Solano [verfasserIn] Edgar Ledesma-Martínez [verfasserIn] Angel de Jesus Delgado-Bordonave [verfasserIn] Ramón Marcos Soto-Hernández [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2017

Schlagwörter:	gisem chayote cytotoxicity differential hplc

Übergeordnetes Werk:	In: Pharmaceutical Biology - Taylor & Francis Group, 2017, 55(2017), 1, Seite 1638-1645
Übergeordnetes Werk:	volume:55 ; year:2017 ; number:1 ; pages:1638-1645

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.1080/13880209.2017.1316746

Katalog-ID:	DOAJ052745562

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520			\|a Context: Sechium edule (Jacq.) Sw. (Cucurbitaceae) is used in ethnomedicine, but the diversity of the varietal groups of this species has not often been considered. This is important because we previously reported that different variety of species exhibit different activities across different tumor cell lines. Objective: This study investigates the chemical composition and biological activities of extracts obtained from S. edule var. nigrum spinosum. Materials and methods: The leukemia P388 cell line and mononuclear bone marrow cells (MNCBMs) were treated with the extract at a concentration ranging from 40 to 2370 μg/mL for cytotoxicity and viability assays. CD-1 mice were treated with 8–5000 mg/kg extract and monitored every hour for the first 24 h and subsequently for seven days for signs of toxicity (LD50). In addition, the chromatographic profile of the extract was determined by HPLC. Results: The extract inhibits the proliferation of both P388 cells and MNCBMs, with IC50 values of 927 and 1911 μg/mL, respectively, but reduced the viability and induced the apoptosis of only leukemia cells. The LD50 was higher than 5000 mg/kg, and this concentration did not alter the blood chemistry or cell count but doubled the mitotic index in the bone marrow. The HPLC showed the presence of cucurbitacins, phloridzin, naringenin, phloretin, apigenin, and gallic, chlorogenic, vanillic, p-hydroxybenzoic, caffeic, and p-coumaric acids. Discussion and conclusion: Sechium edule var. nigrum spinosum contains bioactive compounds that explain the antiproliferative and nutraceutical activities, and its lack of physiological side effects constitutes an added value to a widely consumed vegetable.
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allfields	10.1080/13880209.2017.1316746 doi (DE-627)DOAJ052745562 (DE-599)DOAJf2eb7ca06750453f9a49e78098fcab37 DE-627 ger DE-627 rakwb eng RM1-950 Itzen Aguiñiga-Sánchez verfasserin aut Chemical analyses and in vitro and in vivo toxicity of fruit methanol extract of Sechium edule var. nigrum spinosum 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Context: Sechium edule (Jacq.) Sw. (Cucurbitaceae) is used in ethnomedicine, but the diversity of the varietal groups of this species has not often been considered. This is important because we previously reported that different variety of species exhibit different activities across different tumor cell lines. Objective: This study investigates the chemical composition and biological activities of extracts obtained from S. edule var. nigrum spinosum. Materials and methods: The leukemia P388 cell line and mononuclear bone marrow cells (MNCBMs) were treated with the extract at a concentration ranging from 40 to 2370 μg/mL for cytotoxicity and viability assays. CD-1 mice were treated with 8–5000 mg/kg extract and monitored every hour for the first 24 h and subsequently for seven days for signs of toxicity (LD50). In addition, the chromatographic profile of the extract was determined by HPLC. Results: The extract inhibits the proliferation of both P388 cells and MNCBMs, with IC50 values of 927 and 1911 μg/mL, respectively, but reduced the viability and induced the apoptosis of only leukemia cells. The LD50 was higher than 5000 mg/kg, and this concentration did not alter the blood chemistry or cell count but doubled the mitotic index in the bone marrow. The HPLC showed the presence of cucurbitacins, phloridzin, naringenin, phloretin, apigenin, and gallic, chlorogenic, vanillic, p-hydroxybenzoic, caffeic, and p-coumaric acids. Discussion and conclusion: Sechium edule var. nigrum spinosum contains bioactive compounds that explain the antiproliferative and nutraceutical activities, and its lack of physiological side effects constitutes an added value to a widely consumed vegetable. gisem chayote cytotoxicity differential hplc Therapeutics. Pharmacology Jorge Cadena-Íñiguez verfasserin aut Edelmiro Santiago-Osorio verfasserin aut Guadalupe Gómez-García verfasserin aut Víctor Manuel Mendoza-Núñez verfasserin aut Juana Rosado-Pérez verfasserin aut Mirna Ruíz-Ramos verfasserin aut Víctor Manuel Cisneros-Solano verfasserin aut Edgar Ledesma-Martínez verfasserin aut Angel de Jesus Delgado-Bordonave verfasserin aut Ramón Marcos Soto-Hernández verfasserin aut In Pharmaceutical Biology Taylor & Francis Group, 2017 55(2017), 1, Seite 1638-1645 (DE-627)300595905 (DE-600)1483151-X 17445116 nnns volume:55 year:2017 number:1 pages:1638-1645 https://doi.org/10.1080/13880209.2017.1316746 kostenfrei https://doaj.org/article/f2eb7ca06750453f9a49e78098fcab37 kostenfrei http://dx.doi.org/10.1080/13880209.2017.1316746 kostenfrei https://doaj.org/toc/1388-0209 Journal toc kostenfrei https://doaj.org/toc/1744-5116 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_1200 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 55 2017 1 1638-1645
spelling	10.1080/13880209.2017.1316746 doi (DE-627)DOAJ052745562 (DE-599)DOAJf2eb7ca06750453f9a49e78098fcab37 DE-627 ger DE-627 rakwb eng RM1-950 Itzen Aguiñiga-Sánchez verfasserin aut Chemical analyses and in vitro and in vivo toxicity of fruit methanol extract of Sechium edule var. nigrum spinosum 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Context: Sechium edule (Jacq.) Sw. (Cucurbitaceae) is used in ethnomedicine, but the diversity of the varietal groups of this species has not often been considered. This is important because we previously reported that different variety of species exhibit different activities across different tumor cell lines. Objective: This study investigates the chemical composition and biological activities of extracts obtained from S. edule var. nigrum spinosum. Materials and methods: The leukemia P388 cell line and mononuclear bone marrow cells (MNCBMs) were treated with the extract at a concentration ranging from 40 to 2370 μg/mL for cytotoxicity and viability assays. CD-1 mice were treated with 8–5000 mg/kg extract and monitored every hour for the first 24 h and subsequently for seven days for signs of toxicity (LD50). In addition, the chromatographic profile of the extract was determined by HPLC. Results: The extract inhibits the proliferation of both P388 cells and MNCBMs, with IC50 values of 927 and 1911 μg/mL, respectively, but reduced the viability and induced the apoptosis of only leukemia cells. The LD50 was higher than 5000 mg/kg, and this concentration did not alter the blood chemistry or cell count but doubled the mitotic index in the bone marrow. The HPLC showed the presence of cucurbitacins, phloridzin, naringenin, phloretin, apigenin, and gallic, chlorogenic, vanillic, p-hydroxybenzoic, caffeic, and p-coumaric acids. Discussion and conclusion: Sechium edule var. nigrum spinosum contains bioactive compounds that explain the antiproliferative and nutraceutical activities, and its lack of physiological side effects constitutes an added value to a widely consumed vegetable. gisem chayote cytotoxicity differential hplc Therapeutics. Pharmacology Jorge Cadena-Íñiguez verfasserin aut Edelmiro Santiago-Osorio verfasserin aut Guadalupe Gómez-García verfasserin aut Víctor Manuel Mendoza-Núñez verfasserin aut Juana Rosado-Pérez verfasserin aut Mirna Ruíz-Ramos verfasserin aut Víctor Manuel Cisneros-Solano verfasserin aut Edgar Ledesma-Martínez verfasserin aut Angel de Jesus Delgado-Bordonave verfasserin aut Ramón Marcos Soto-Hernández verfasserin aut In Pharmaceutical Biology Taylor & Francis Group, 2017 55(2017), 1, Seite 1638-1645 (DE-627)300595905 (DE-600)1483151-X 17445116 nnns volume:55 year:2017 number:1 pages:1638-1645 https://doi.org/10.1080/13880209.2017.1316746 kostenfrei https://doaj.org/article/f2eb7ca06750453f9a49e78098fcab37 kostenfrei http://dx.doi.org/10.1080/13880209.2017.1316746 kostenfrei https://doaj.org/toc/1388-0209 Journal toc kostenfrei https://doaj.org/toc/1744-5116 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_1200 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 55 2017 1 1638-1645
allfields_unstemmed	10.1080/13880209.2017.1316746 doi (DE-627)DOAJ052745562 (DE-599)DOAJf2eb7ca06750453f9a49e78098fcab37 DE-627 ger DE-627 rakwb eng RM1-950 Itzen Aguiñiga-Sánchez verfasserin aut Chemical analyses and in vitro and in vivo toxicity of fruit methanol extract of Sechium edule var. nigrum spinosum 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Context: Sechium edule (Jacq.) Sw. (Cucurbitaceae) is used in ethnomedicine, but the diversity of the varietal groups of this species has not often been considered. This is important because we previously reported that different variety of species exhibit different activities across different tumor cell lines. Objective: This study investigates the chemical composition and biological activities of extracts obtained from S. edule var. nigrum spinosum. Materials and methods: The leukemia P388 cell line and mononuclear bone marrow cells (MNCBMs) were treated with the extract at a concentration ranging from 40 to 2370 μg/mL for cytotoxicity and viability assays. CD-1 mice were treated with 8–5000 mg/kg extract and monitored every hour for the first 24 h and subsequently for seven days for signs of toxicity (LD50). In addition, the chromatographic profile of the extract was determined by HPLC. Results: The extract inhibits the proliferation of both P388 cells and MNCBMs, with IC50 values of 927 and 1911 μg/mL, respectively, but reduced the viability and induced the apoptosis of only leukemia cells. The LD50 was higher than 5000 mg/kg, and this concentration did not alter the blood chemistry or cell count but doubled the mitotic index in the bone marrow. The HPLC showed the presence of cucurbitacins, phloridzin, naringenin, phloretin, apigenin, and gallic, chlorogenic, vanillic, p-hydroxybenzoic, caffeic, and p-coumaric acids. Discussion and conclusion: Sechium edule var. nigrum spinosum contains bioactive compounds that explain the antiproliferative and nutraceutical activities, and its lack of physiological side effects constitutes an added value to a widely consumed vegetable. gisem chayote cytotoxicity differential hplc Therapeutics. Pharmacology Jorge Cadena-Íñiguez verfasserin aut Edelmiro Santiago-Osorio verfasserin aut Guadalupe Gómez-García verfasserin aut Víctor Manuel Mendoza-Núñez verfasserin aut Juana Rosado-Pérez verfasserin aut Mirna Ruíz-Ramos verfasserin aut Víctor Manuel Cisneros-Solano verfasserin aut Edgar Ledesma-Martínez verfasserin aut Angel de Jesus Delgado-Bordonave verfasserin aut Ramón Marcos Soto-Hernández verfasserin aut In Pharmaceutical Biology Taylor & Francis Group, 2017 55(2017), 1, Seite 1638-1645 (DE-627)300595905 (DE-600)1483151-X 17445116 nnns volume:55 year:2017 number:1 pages:1638-1645 https://doi.org/10.1080/13880209.2017.1316746 kostenfrei https://doaj.org/article/f2eb7ca06750453f9a49e78098fcab37 kostenfrei http://dx.doi.org/10.1080/13880209.2017.1316746 kostenfrei https://doaj.org/toc/1388-0209 Journal toc kostenfrei https://doaj.org/toc/1744-5116 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_1200 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 55 2017 1 1638-1645
allfieldsGer	10.1080/13880209.2017.1316746 doi (DE-627)DOAJ052745562 (DE-599)DOAJf2eb7ca06750453f9a49e78098fcab37 DE-627 ger DE-627 rakwb eng RM1-950 Itzen Aguiñiga-Sánchez verfasserin aut Chemical analyses and in vitro and in vivo toxicity of fruit methanol extract of Sechium edule var. nigrum spinosum 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Context: Sechium edule (Jacq.) Sw. (Cucurbitaceae) is used in ethnomedicine, but the diversity of the varietal groups of this species has not often been considered. This is important because we previously reported that different variety of species exhibit different activities across different tumor cell lines. Objective: This study investigates the chemical composition and biological activities of extracts obtained from S. edule var. nigrum spinosum. Materials and methods: The leukemia P388 cell line and mononuclear bone marrow cells (MNCBMs) were treated with the extract at a concentration ranging from 40 to 2370 μg/mL for cytotoxicity and viability assays. CD-1 mice were treated with 8–5000 mg/kg extract and monitored every hour for the first 24 h and subsequently for seven days for signs of toxicity (LD50). In addition, the chromatographic profile of the extract was determined by HPLC. Results: The extract inhibits the proliferation of both P388 cells and MNCBMs, with IC50 values of 927 and 1911 μg/mL, respectively, but reduced the viability and induced the apoptosis of only leukemia cells. The LD50 was higher than 5000 mg/kg, and this concentration did not alter the blood chemistry or cell count but doubled the mitotic index in the bone marrow. The HPLC showed the presence of cucurbitacins, phloridzin, naringenin, phloretin, apigenin, and gallic, chlorogenic, vanillic, p-hydroxybenzoic, caffeic, and p-coumaric acids. Discussion and conclusion: Sechium edule var. nigrum spinosum contains bioactive compounds that explain the antiproliferative and nutraceutical activities, and its lack of physiological side effects constitutes an added value to a widely consumed vegetable. gisem chayote cytotoxicity differential hplc Therapeutics. Pharmacology Jorge Cadena-Íñiguez verfasserin aut Edelmiro Santiago-Osorio verfasserin aut Guadalupe Gómez-García verfasserin aut Víctor Manuel Mendoza-Núñez verfasserin aut Juana Rosado-Pérez verfasserin aut Mirna Ruíz-Ramos verfasserin aut Víctor Manuel Cisneros-Solano verfasserin aut Edgar Ledesma-Martínez verfasserin aut Angel de Jesus Delgado-Bordonave verfasserin aut Ramón Marcos Soto-Hernández verfasserin aut In Pharmaceutical Biology Taylor & Francis Group, 2017 55(2017), 1, Seite 1638-1645 (DE-627)300595905 (DE-600)1483151-X 17445116 nnns volume:55 year:2017 number:1 pages:1638-1645 https://doi.org/10.1080/13880209.2017.1316746 kostenfrei https://doaj.org/article/f2eb7ca06750453f9a49e78098fcab37 kostenfrei http://dx.doi.org/10.1080/13880209.2017.1316746 kostenfrei https://doaj.org/toc/1388-0209 Journal toc kostenfrei https://doaj.org/toc/1744-5116 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_1200 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 55 2017 1 1638-1645
allfieldsSound	10.1080/13880209.2017.1316746 doi (DE-627)DOAJ052745562 (DE-599)DOAJf2eb7ca06750453f9a49e78098fcab37 DE-627 ger DE-627 rakwb eng RM1-950 Itzen Aguiñiga-Sánchez verfasserin aut Chemical analyses and in vitro and in vivo toxicity of fruit methanol extract of Sechium edule var. nigrum spinosum 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Context: Sechium edule (Jacq.) Sw. (Cucurbitaceae) is used in ethnomedicine, but the diversity of the varietal groups of this species has not often been considered. This is important because we previously reported that different variety of species exhibit different activities across different tumor cell lines. Objective: This study investigates the chemical composition and biological activities of extracts obtained from S. edule var. nigrum spinosum. Materials and methods: The leukemia P388 cell line and mononuclear bone marrow cells (MNCBMs) were treated with the extract at a concentration ranging from 40 to 2370 μg/mL for cytotoxicity and viability assays. CD-1 mice were treated with 8–5000 mg/kg extract and monitored every hour for the first 24 h and subsequently for seven days for signs of toxicity (LD50). In addition, the chromatographic profile of the extract was determined by HPLC. Results: The extract inhibits the proliferation of both P388 cells and MNCBMs, with IC50 values of 927 and 1911 μg/mL, respectively, but reduced the viability and induced the apoptosis of only leukemia cells. The LD50 was higher than 5000 mg/kg, and this concentration did not alter the blood chemistry or cell count but doubled the mitotic index in the bone marrow. The HPLC showed the presence of cucurbitacins, phloridzin, naringenin, phloretin, apigenin, and gallic, chlorogenic, vanillic, p-hydroxybenzoic, caffeic, and p-coumaric acids. Discussion and conclusion: Sechium edule var. nigrum spinosum contains bioactive compounds that explain the antiproliferative and nutraceutical activities, and its lack of physiological side effects constitutes an added value to a widely consumed vegetable. gisem chayote cytotoxicity differential hplc Therapeutics. Pharmacology Jorge Cadena-Íñiguez verfasserin aut Edelmiro Santiago-Osorio verfasserin aut Guadalupe Gómez-García verfasserin aut Víctor Manuel Mendoza-Núñez verfasserin aut Juana Rosado-Pérez verfasserin aut Mirna Ruíz-Ramos verfasserin aut Víctor Manuel Cisneros-Solano verfasserin aut Edgar Ledesma-Martínez verfasserin aut Angel de Jesus Delgado-Bordonave verfasserin aut Ramón Marcos Soto-Hernández verfasserin aut In Pharmaceutical Biology Taylor & Francis Group, 2017 55(2017), 1, Seite 1638-1645 (DE-627)300595905 (DE-600)1483151-X 17445116 nnns volume:55 year:2017 number:1 pages:1638-1645 https://doi.org/10.1080/13880209.2017.1316746 kostenfrei https://doaj.org/article/f2eb7ca06750453f9a49e78098fcab37 kostenfrei http://dx.doi.org/10.1080/13880209.2017.1316746 kostenfrei https://doaj.org/toc/1388-0209 Journal toc kostenfrei https://doaj.org/toc/1744-5116 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_1200 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 55 2017 1 1638-1645
language	English
source	In Pharmaceutical Biology 55(2017), 1, Seite 1638-1645 volume:55 year:2017 number:1 pages:1638-1645
sourceStr	In Pharmaceutical Biology 55(2017), 1, Seite 1638-1645 volume:55 year:2017 number:1 pages:1638-1645
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	gisem chayote cytotoxicity differential hplc Therapeutics. Pharmacology
isfreeaccess_bool	true
container_title	Pharmaceutical Biology
authorswithroles_txt_mv	Itzen Aguiñiga-Sánchez @@aut@@ Jorge Cadena-Íñiguez @@aut@@ Edelmiro Santiago-Osorio @@aut@@ Guadalupe Gómez-García @@aut@@ Víctor Manuel Mendoza-Núñez @@aut@@ Juana Rosado-Pérez @@aut@@ Mirna Ruíz-Ramos @@aut@@ Víctor Manuel Cisneros-Solano @@aut@@ Edgar Ledesma-Martínez @@aut@@ Angel de Jesus Delgado-Bordonave @@aut@@ Ramón Marcos Soto-Hernández @@aut@@
publishDateDaySort_date	2017-01-01T00:00:00Z
hierarchy_top_id	300595905
id	DOAJ052745562
language_de	englisch
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Sw. (Cucurbitaceae) is used in ethnomedicine, but the diversity of the varietal groups of this species has not often been considered. This is important because we previously reported that different variety of species exhibit different activities across different tumor cell lines. Objective: This study investigates the chemical composition and biological activities of extracts obtained from S. edule var. nigrum spinosum. Materials and methods: The leukemia P388 cell line and mononuclear bone marrow cells (MNCBMs) were treated with the extract at a concentration ranging from 40 to 2370 μg/mL for cytotoxicity and viability assays. CD-1 mice were treated with 8–5000 mg/kg extract and monitored every hour for the first 24 h and subsequently for seven days for signs of toxicity (LD50). In addition, the chromatographic profile of the extract was determined by HPLC. Results: The extract inhibits the proliferation of both P388 cells and MNCBMs, with IC50 values of 927 and 1911 μg/mL, respectively, but reduced the viability and induced the apoptosis of only leukemia cells. The LD50 was higher than 5000 mg/kg, and this concentration did not alter the blood chemistry or cell count but doubled the mitotic index in the bone marrow. The HPLC showed the presence of cucurbitacins, phloridzin, naringenin, phloretin, apigenin, and gallic, chlorogenic, vanillic, p-hydroxybenzoic, caffeic, and p-coumaric acids. 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callnumber-first	R - Medicine
author	Itzen Aguiñiga-Sánchez
spellingShingle	Itzen Aguiñiga-Sánchez misc RM1-950 misc gisem misc chayote misc cytotoxicity misc differential misc hplc misc Therapeutics. Pharmacology Chemical analyses and in vitro and in vivo toxicity of fruit methanol extract of Sechium edule var. nigrum spinosum
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topic_title	RM1-950 Chemical analyses and in vitro and in vivo toxicity of fruit methanol extract of Sechium edule var. nigrum spinosum gisem chayote cytotoxicity differential hplc
topic	misc RM1-950 misc gisem misc chayote misc cytotoxicity misc differential misc hplc misc Therapeutics. Pharmacology
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title	Chemical analyses and in vitro and in vivo toxicity of fruit methanol extract of Sechium edule var. nigrum spinosum
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title_full	Chemical analyses and in vitro and in vivo toxicity of fruit methanol extract of Sechium edule var. nigrum spinosum
author_sort	Itzen Aguiñiga-Sánchez
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author_browse	Itzen Aguiñiga-Sánchez Jorge Cadena-Íñiguez Edelmiro Santiago-Osorio Guadalupe Gómez-García Víctor Manuel Mendoza-Núñez Juana Rosado-Pérez Mirna Ruíz-Ramos Víctor Manuel Cisneros-Solano Edgar Ledesma-Martínez Angel de Jesus Delgado-Bordonave Ramón Marcos Soto-Hernández
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doi_str_mv	10.1080/13880209.2017.1316746
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title_sort	chemical analyses and in vitro and in vivo toxicity of fruit methanol extract of sechium edule var. nigrum spinosum
callnumber	RM1-950
title_auth	Chemical analyses and in vitro and in vivo toxicity of fruit methanol extract of Sechium edule var. nigrum spinosum
abstract	Context: Sechium edule (Jacq.) Sw. (Cucurbitaceae) is used in ethnomedicine, but the diversity of the varietal groups of this species has not often been considered. This is important because we previously reported that different variety of species exhibit different activities across different tumor cell lines. Objective: This study investigates the chemical composition and biological activities of extracts obtained from S. edule var. nigrum spinosum. Materials and methods: The leukemia P388 cell line and mononuclear bone marrow cells (MNCBMs) were treated with the extract at a concentration ranging from 40 to 2370 μg/mL for cytotoxicity and viability assays. CD-1 mice were treated with 8–5000 mg/kg extract and monitored every hour for the first 24 h and subsequently for seven days for signs of toxicity (LD50). In addition, the chromatographic profile of the extract was determined by HPLC. Results: The extract inhibits the proliferation of both P388 cells and MNCBMs, with IC50 values of 927 and 1911 μg/mL, respectively, but reduced the viability and induced the apoptosis of only leukemia cells. The LD50 was higher than 5000 mg/kg, and this concentration did not alter the blood chemistry or cell count but doubled the mitotic index in the bone marrow. The HPLC showed the presence of cucurbitacins, phloridzin, naringenin, phloretin, apigenin, and gallic, chlorogenic, vanillic, p-hydroxybenzoic, caffeic, and p-coumaric acids. Discussion and conclusion: Sechium edule var. nigrum spinosum contains bioactive compounds that explain the antiproliferative and nutraceutical activities, and its lack of physiological side effects constitutes an added value to a widely consumed vegetable.
abstractGer	Context: Sechium edule (Jacq.) Sw. (Cucurbitaceae) is used in ethnomedicine, but the diversity of the varietal groups of this species has not often been considered. This is important because we previously reported that different variety of species exhibit different activities across different tumor cell lines. Objective: This study investigates the chemical composition and biological activities of extracts obtained from S. edule var. nigrum spinosum. Materials and methods: The leukemia P388 cell line and mononuclear bone marrow cells (MNCBMs) were treated with the extract at a concentration ranging from 40 to 2370 μg/mL for cytotoxicity and viability assays. CD-1 mice were treated with 8–5000 mg/kg extract and monitored every hour for the first 24 h and subsequently for seven days for signs of toxicity (LD50). In addition, the chromatographic profile of the extract was determined by HPLC. Results: The extract inhibits the proliferation of both P388 cells and MNCBMs, with IC50 values of 927 and 1911 μg/mL, respectively, but reduced the viability and induced the apoptosis of only leukemia cells. The LD50 was higher than 5000 mg/kg, and this concentration did not alter the blood chemistry or cell count but doubled the mitotic index in the bone marrow. The HPLC showed the presence of cucurbitacins, phloridzin, naringenin, phloretin, apigenin, and gallic, chlorogenic, vanillic, p-hydroxybenzoic, caffeic, and p-coumaric acids. Discussion and conclusion: Sechium edule var. nigrum spinosum contains bioactive compounds that explain the antiproliferative and nutraceutical activities, and its lack of physiological side effects constitutes an added value to a widely consumed vegetable.
abstract_unstemmed	Context: Sechium edule (Jacq.) Sw. (Cucurbitaceae) is used in ethnomedicine, but the diversity of the varietal groups of this species has not often been considered. This is important because we previously reported that different variety of species exhibit different activities across different tumor cell lines. Objective: This study investigates the chemical composition and biological activities of extracts obtained from S. edule var. nigrum spinosum. Materials and methods: The leukemia P388 cell line and mononuclear bone marrow cells (MNCBMs) were treated with the extract at a concentration ranging from 40 to 2370 μg/mL for cytotoxicity and viability assays. CD-1 mice were treated with 8–5000 mg/kg extract and monitored every hour for the first 24 h and subsequently for seven days for signs of toxicity (LD50). In addition, the chromatographic profile of the extract was determined by HPLC. Results: The extract inhibits the proliferation of both P388 cells and MNCBMs, with IC50 values of 927 and 1911 μg/mL, respectively, but reduced the viability and induced the apoptosis of only leukemia cells. The LD50 was higher than 5000 mg/kg, and this concentration did not alter the blood chemistry or cell count but doubled the mitotic index in the bone marrow. The HPLC showed the presence of cucurbitacins, phloridzin, naringenin, phloretin, apigenin, and gallic, chlorogenic, vanillic, p-hydroxybenzoic, caffeic, and p-coumaric acids. Discussion and conclusion: Sechium edule var. nigrum spinosum contains bioactive compounds that explain the antiproliferative and nutraceutical activities, and its lack of physiological side effects constitutes an added value to a widely consumed vegetable.
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title_short	Chemical analyses and in vitro and in vivo toxicity of fruit methanol extract of Sechium edule var. nigrum spinosum
url	https://doi.org/10.1080/13880209.2017.1316746 https://doaj.org/article/f2eb7ca06750453f9a49e78098fcab37 http://dx.doi.org/10.1080/13880209.2017.1316746 https://doaj.org/toc/1388-0209 https://doaj.org/toc/1744-5116
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author2	Jorge Cadena-Íñiguez Edelmiro Santiago-Osorio Guadalupe Gómez-García Víctor Manuel Mendoza-Núñez Juana Rosado-Pérez Mirna Ruíz-Ramos Víctor Manuel Cisneros-Solano Edgar Ledesma-Martínez Angel de Jesus Delgado-Bordonave Ramón Marcos Soto-Hernández
author2Str	Jorge Cadena-Íñiguez Edelmiro Santiago-Osorio Guadalupe Gómez-García Víctor Manuel Mendoza-Núñez Juana Rosado-Pérez Mirna Ruíz-Ramos Víctor Manuel Cisneros-Solano Edgar Ledesma-Martínez Angel de Jesus Delgado-Bordonave Ramón Marcos Soto-Hernández
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doi_str	10.1080/13880209.2017.1316746
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up_date	2024-07-03T13:49:18.335Z
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Sw. (Cucurbitaceae) is used in ethnomedicine, but the diversity of the varietal groups of this species has not often been considered. This is important because we previously reported that different variety of species exhibit different activities across different tumor cell lines. Objective: This study investigates the chemical composition and biological activities of extracts obtained from S. edule var. nigrum spinosum. Materials and methods: The leukemia P388 cell line and mononuclear bone marrow cells (MNCBMs) were treated with the extract at a concentration ranging from 40 to 2370 μg/mL for cytotoxicity and viability assays. CD-1 mice were treated with 8–5000 mg/kg extract and monitored every hour for the first 24 h and subsequently for seven days for signs of toxicity (LD50). In addition, the chromatographic profile of the extract was determined by HPLC. Results: The extract inhibits the proliferation of both P388 cells and MNCBMs, with IC50 values of 927 and 1911 μg/mL, respectively, but reduced the viability and induced the apoptosis of only leukemia cells. The LD50 was higher than 5000 mg/kg, and this concentration did not alter the blood chemistry or cell count but doubled the mitotic index in the bone marrow. The HPLC showed the presence of cucurbitacins, phloridzin, naringenin, phloretin, apigenin, and gallic, chlorogenic, vanillic, p-hydroxybenzoic, caffeic, and p-coumaric acids. Discussion and conclusion: Sechium edule var. nigrum spinosum contains bioactive compounds that explain the antiproliferative and nutraceutical activities, and its lack of physiological side effects constitutes an added value to a widely consumed vegetable.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">gisem</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">chayote</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">cytotoxicity</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">differential</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">hplc</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Therapeutics. 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Chemical analyses and in vitro and in vivo toxicity of fruit methanol extract of Sechium edule var. nigrum spinosum

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