Characterisation of the Antibiotic Profile of <i<Lysobacter capsici</i< AZ78, an Effective Biological Control Agent of Plant Pathogenic Microorganisms

Determining the mode of action of microbial biocontrol agents plays a key role in their development and registration as commercial biopesticides. The biocontrol rhizobacterium <i<Lysobacter capsici</i< AZ78 (AZ78) is able to inhibit a vast array of plant pathogenic oomycetes and Gram-positive bacteria due to the release of antimicrobial secondary metabolites. A combination of MALDI-qTOF-MSI and UHPLC-HRMS/M was applied to finely dissect the AZ78 metabolome and identify the main secondary metabolites involved in the inhibition of plant pathogenic microorganisms. Under nutritionally limited conditions, MALDI-qTOF-MSI revealed that AZ78 is able to release a relevant number of antimicrobial secondary metabolites belonging to the families of 2,5-diketopiperazines, cyclic lipodepsipeptides, macrolactones and macrolides. In vitro tests confirmed the presence of secondary metabolites toxic against <i<Pythium ultimum</i< and <i<Rhodococcus fascians</i< in AZ78 cell-free extracts. Subsequently, UHPLC-HRMS/MS was used to confirm the results achieved with MALDI-qTOF-MSI and investigate for further putative antimicrobial secondary metabolites known to be produced by <i<Lysobacter</i< spp. This technique confirmed the presence of several 2,5-diketopiperazines in AZ78 cell-free extracts and provided the first evidence of the production of the cyclic depsipeptide WAP-8294A2 in a member of <i<L. capsici</i< species. Moreover, UHPLC-HRMS/MS confirmed the presence of dihydromaltophilin/Heat Stable Antifungal Factor (HSAF) in AZ78 cell-free extracts. Due to the production of HSAF by AZ78, cell-free supernatants were effective in controlling <i<Plasmopara viticola</i< on grapevine leaf disks after exposure to high temperatures. Overall, our work determined the main secondary metabolites involved in the biocontrol activity of AZ78 against plant pathogenic oomycetes and Gram-positive bacteria. These results might be useful for the future development of this bacterial strain as the active ingredient of a microbial biopesticide that might contribute to a reduction in the chemical input in agriculture. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Francesca Brescia [verfasserIn] Anthi Vlassi [verfasserIn] Ana Bejarano [verfasserIn] Bernard Seidl [verfasserIn] Martina Marchetti-Deschmann [verfasserIn] Rainer Schuhmacher [verfasserIn] Gerardo Puopolo [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	WAP-8294A2 dihydromaltophilin (HSAF) 2,5-diketopiperazines MALDI-qTOF-MSI UHPLC-HRMS/MS

Übergeordnetes Werk:	In: Microorganisms - MDPI AG, 2013, 9(2021), 6, p 1320
Übergeordnetes Werk:	volume:9 ; year:2021 ; number:6, p 1320

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/microorganisms9061320

Katalog-ID:	DOAJ052856402

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520			\|a Determining the mode of action of microbial biocontrol agents plays a key role in their development and registration as commercial biopesticides. The biocontrol rhizobacterium <i<Lysobacter capsici</i< AZ78 (AZ78) is able to inhibit a vast array of plant pathogenic oomycetes and Gram-positive bacteria due to the release of antimicrobial secondary metabolites. A combination of MALDI-qTOF-MSI and UHPLC-HRMS/M was applied to finely dissect the AZ78 metabolome and identify the main secondary metabolites involved in the inhibition of plant pathogenic microorganisms. Under nutritionally limited conditions, MALDI-qTOF-MSI revealed that AZ78 is able to release a relevant number of antimicrobial secondary metabolites belonging to the families of 2,5-diketopiperazines, cyclic lipodepsipeptides, macrolactones and macrolides. In vitro tests confirmed the presence of secondary metabolites toxic against <i<Pythium ultimum</i< and <i<Rhodococcus fascians</i< in AZ78 cell-free extracts. Subsequently, UHPLC-HRMS/MS was used to confirm the results achieved with MALDI-qTOF-MSI and investigate for further putative antimicrobial secondary metabolites known to be produced by <i<Lysobacter</i< spp. This technique confirmed the presence of several 2,5-diketopiperazines in AZ78 cell-free extracts and provided the first evidence of the production of the cyclic depsipeptide WAP-8294A2 in a member of <i<L. capsici</i< species. Moreover, UHPLC-HRMS/MS confirmed the presence of dihydromaltophilin/Heat Stable Antifungal Factor (HSAF) in AZ78 cell-free extracts. Due to the production of HSAF by AZ78, cell-free supernatants were effective in controlling <i<Plasmopara viticola</i< on grapevine leaf disks after exposure to high temperatures. Overall, our work determined the main secondary metabolites involved in the biocontrol activity of AZ78 against plant pathogenic oomycetes and Gram-positive bacteria. These results might be useful for the future development of this bacterial strain as the active ingredient of a microbial biopesticide that might contribute to a reduction in the chemical input in agriculture.
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allfields	10.3390/microorganisms9061320 doi (DE-627)DOAJ052856402 (DE-599)DOAJba99b35b77b443c48b313e944b4f41ec DE-627 ger DE-627 rakwb eng QH301-705.5 Francesca Brescia verfasserin aut Characterisation of the Antibiotic Profile of <i<Lysobacter capsici</i< AZ78, an Effective Biological Control Agent of Plant Pathogenic Microorganisms 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Determining the mode of action of microbial biocontrol agents plays a key role in their development and registration as commercial biopesticides. The biocontrol rhizobacterium <i<Lysobacter capsici</i< AZ78 (AZ78) is able to inhibit a vast array of plant pathogenic oomycetes and Gram-positive bacteria due to the release of antimicrobial secondary metabolites. A combination of MALDI-qTOF-MSI and UHPLC-HRMS/M was applied to finely dissect the AZ78 metabolome and identify the main secondary metabolites involved in the inhibition of plant pathogenic microorganisms. Under nutritionally limited conditions, MALDI-qTOF-MSI revealed that AZ78 is able to release a relevant number of antimicrobial secondary metabolites belonging to the families of 2,5-diketopiperazines, cyclic lipodepsipeptides, macrolactones and macrolides. In vitro tests confirmed the presence of secondary metabolites toxic against <i<Pythium ultimum</i< and <i<Rhodococcus fascians</i< in AZ78 cell-free extracts. Subsequently, UHPLC-HRMS/MS was used to confirm the results achieved with MALDI-qTOF-MSI and investigate for further putative antimicrobial secondary metabolites known to be produced by <i<Lysobacter</i< spp. This technique confirmed the presence of several 2,5-diketopiperazines in AZ78 cell-free extracts and provided the first evidence of the production of the cyclic depsipeptide WAP-8294A2 in a member of <i<L. capsici</i< species. Moreover, UHPLC-HRMS/MS confirmed the presence of dihydromaltophilin/Heat Stable Antifungal Factor (HSAF) in AZ78 cell-free extracts. Due to the production of HSAF by AZ78, cell-free supernatants were effective in controlling <i<Plasmopara viticola</i< on grapevine leaf disks after exposure to high temperatures. Overall, our work determined the main secondary metabolites involved in the biocontrol activity of AZ78 against plant pathogenic oomycetes and Gram-positive bacteria. These results might be useful for the future development of this bacterial strain as the active ingredient of a microbial biopesticide that might contribute to a reduction in the chemical input in agriculture. <i<Lysobacter capsici</i< WAP-8294A2 dihydromaltophilin (HSAF) 2,5-diketopiperazines MALDI-qTOF-MSI UHPLC-HRMS/MS Biology (General) Anthi Vlassi verfasserin aut Ana Bejarano verfasserin aut Bernard Seidl verfasserin aut Martina Marchetti-Deschmann verfasserin aut Rainer Schuhmacher verfasserin aut Gerardo Puopolo verfasserin aut In Microorganisms MDPI AG, 2013 9(2021), 6, p 1320 (DE-627)750370696 (DE-600)2720891-6 20762607 nnns volume:9 year:2021 number:6, p 1320 https://doi.org/10.3390/microorganisms9061320 kostenfrei https://doaj.org/article/ba99b35b77b443c48b313e944b4f41ec kostenfrei https://www.mdpi.com/2076-2607/9/6/1320 kostenfrei https://doaj.org/toc/2076-2607 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 6, p 1320
spelling	10.3390/microorganisms9061320 doi (DE-627)DOAJ052856402 (DE-599)DOAJba99b35b77b443c48b313e944b4f41ec DE-627 ger DE-627 rakwb eng QH301-705.5 Francesca Brescia verfasserin aut Characterisation of the Antibiotic Profile of <i<Lysobacter capsici</i< AZ78, an Effective Biological Control Agent of Plant Pathogenic Microorganisms 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Determining the mode of action of microbial biocontrol agents plays a key role in their development and registration as commercial biopesticides. The biocontrol rhizobacterium <i<Lysobacter capsici</i< AZ78 (AZ78) is able to inhibit a vast array of plant pathogenic oomycetes and Gram-positive bacteria due to the release of antimicrobial secondary metabolites. A combination of MALDI-qTOF-MSI and UHPLC-HRMS/M was applied to finely dissect the AZ78 metabolome and identify the main secondary metabolites involved in the inhibition of plant pathogenic microorganisms. Under nutritionally limited conditions, MALDI-qTOF-MSI revealed that AZ78 is able to release a relevant number of antimicrobial secondary metabolites belonging to the families of 2,5-diketopiperazines, cyclic lipodepsipeptides, macrolactones and macrolides. In vitro tests confirmed the presence of secondary metabolites toxic against <i<Pythium ultimum</i< and <i<Rhodococcus fascians</i< in AZ78 cell-free extracts. Subsequently, UHPLC-HRMS/MS was used to confirm the results achieved with MALDI-qTOF-MSI and investigate for further putative antimicrobial secondary metabolites known to be produced by <i<Lysobacter</i< spp. This technique confirmed the presence of several 2,5-diketopiperazines in AZ78 cell-free extracts and provided the first evidence of the production of the cyclic depsipeptide WAP-8294A2 in a member of <i<L. capsici</i< species. Moreover, UHPLC-HRMS/MS confirmed the presence of dihydromaltophilin/Heat Stable Antifungal Factor (HSAF) in AZ78 cell-free extracts. Due to the production of HSAF by AZ78, cell-free supernatants were effective in controlling <i<Plasmopara viticola</i< on grapevine leaf disks after exposure to high temperatures. Overall, our work determined the main secondary metabolites involved in the biocontrol activity of AZ78 against plant pathogenic oomycetes and Gram-positive bacteria. These results might be useful for the future development of this bacterial strain as the active ingredient of a microbial biopesticide that might contribute to a reduction in the chemical input in agriculture. <i<Lysobacter capsici</i< WAP-8294A2 dihydromaltophilin (HSAF) 2,5-diketopiperazines MALDI-qTOF-MSI UHPLC-HRMS/MS Biology (General) Anthi Vlassi verfasserin aut Ana Bejarano verfasserin aut Bernard Seidl verfasserin aut Martina Marchetti-Deschmann verfasserin aut Rainer Schuhmacher verfasserin aut Gerardo Puopolo verfasserin aut In Microorganisms MDPI AG, 2013 9(2021), 6, p 1320 (DE-627)750370696 (DE-600)2720891-6 20762607 nnns volume:9 year:2021 number:6, p 1320 https://doi.org/10.3390/microorganisms9061320 kostenfrei https://doaj.org/article/ba99b35b77b443c48b313e944b4f41ec kostenfrei https://www.mdpi.com/2076-2607/9/6/1320 kostenfrei https://doaj.org/toc/2076-2607 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 6, p 1320
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allfieldsGer	10.3390/microorganisms9061320 doi (DE-627)DOAJ052856402 (DE-599)DOAJba99b35b77b443c48b313e944b4f41ec DE-627 ger DE-627 rakwb eng QH301-705.5 Francesca Brescia verfasserin aut Characterisation of the Antibiotic Profile of <i<Lysobacter capsici</i< AZ78, an Effective Biological Control Agent of Plant Pathogenic Microorganisms 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Determining the mode of action of microbial biocontrol agents plays a key role in their development and registration as commercial biopesticides. The biocontrol rhizobacterium <i<Lysobacter capsici</i< AZ78 (AZ78) is able to inhibit a vast array of plant pathogenic oomycetes and Gram-positive bacteria due to the release of antimicrobial secondary metabolites. A combination of MALDI-qTOF-MSI and UHPLC-HRMS/M was applied to finely dissect the AZ78 metabolome and identify the main secondary metabolites involved in the inhibition of plant pathogenic microorganisms. Under nutritionally limited conditions, MALDI-qTOF-MSI revealed that AZ78 is able to release a relevant number of antimicrobial secondary metabolites belonging to the families of 2,5-diketopiperazines, cyclic lipodepsipeptides, macrolactones and macrolides. In vitro tests confirmed the presence of secondary metabolites toxic against <i<Pythium ultimum</i< and <i<Rhodococcus fascians</i< in AZ78 cell-free extracts. Subsequently, UHPLC-HRMS/MS was used to confirm the results achieved with MALDI-qTOF-MSI and investigate for further putative antimicrobial secondary metabolites known to be produced by <i<Lysobacter</i< spp. This technique confirmed the presence of several 2,5-diketopiperazines in AZ78 cell-free extracts and provided the first evidence of the production of the cyclic depsipeptide WAP-8294A2 in a member of <i<L. capsici</i< species. Moreover, UHPLC-HRMS/MS confirmed the presence of dihydromaltophilin/Heat Stable Antifungal Factor (HSAF) in AZ78 cell-free extracts. Due to the production of HSAF by AZ78, cell-free supernatants were effective in controlling <i<Plasmopara viticola</i< on grapevine leaf disks after exposure to high temperatures. Overall, our work determined the main secondary metabolites involved in the biocontrol activity of AZ78 against plant pathogenic oomycetes and Gram-positive bacteria. These results might be useful for the future development of this bacterial strain as the active ingredient of a microbial biopesticide that might contribute to a reduction in the chemical input in agriculture. <i<Lysobacter capsici</i< WAP-8294A2 dihydromaltophilin (HSAF) 2,5-diketopiperazines MALDI-qTOF-MSI UHPLC-HRMS/MS Biology (General) Anthi Vlassi verfasserin aut Ana Bejarano verfasserin aut Bernard Seidl verfasserin aut Martina Marchetti-Deschmann verfasserin aut Rainer Schuhmacher verfasserin aut Gerardo Puopolo verfasserin aut In Microorganisms MDPI AG, 2013 9(2021), 6, p 1320 (DE-627)750370696 (DE-600)2720891-6 20762607 nnns volume:9 year:2021 number:6, p 1320 https://doi.org/10.3390/microorganisms9061320 kostenfrei https://doaj.org/article/ba99b35b77b443c48b313e944b4f41ec kostenfrei https://www.mdpi.com/2076-2607/9/6/1320 kostenfrei https://doaj.org/toc/2076-2607 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 6, p 1320
allfieldsSound	10.3390/microorganisms9061320 doi (DE-627)DOAJ052856402 (DE-599)DOAJba99b35b77b443c48b313e944b4f41ec DE-627 ger DE-627 rakwb eng QH301-705.5 Francesca Brescia verfasserin aut Characterisation of the Antibiotic Profile of <i<Lysobacter capsici</i< AZ78, an Effective Biological Control Agent of Plant Pathogenic Microorganisms 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Determining the mode of action of microbial biocontrol agents plays a key role in their development and registration as commercial biopesticides. The biocontrol rhizobacterium <i<Lysobacter capsici</i< AZ78 (AZ78) is able to inhibit a vast array of plant pathogenic oomycetes and Gram-positive bacteria due to the release of antimicrobial secondary metabolites. A combination of MALDI-qTOF-MSI and UHPLC-HRMS/M was applied to finely dissect the AZ78 metabolome and identify the main secondary metabolites involved in the inhibition of plant pathogenic microorganisms. Under nutritionally limited conditions, MALDI-qTOF-MSI revealed that AZ78 is able to release a relevant number of antimicrobial secondary metabolites belonging to the families of 2,5-diketopiperazines, cyclic lipodepsipeptides, macrolactones and macrolides. In vitro tests confirmed the presence of secondary metabolites toxic against <i<Pythium ultimum</i< and <i<Rhodococcus fascians</i< in AZ78 cell-free extracts. Subsequently, UHPLC-HRMS/MS was used to confirm the results achieved with MALDI-qTOF-MSI and investigate for further putative antimicrobial secondary metabolites known to be produced by <i<Lysobacter</i< spp. This technique confirmed the presence of several 2,5-diketopiperazines in AZ78 cell-free extracts and provided the first evidence of the production of the cyclic depsipeptide WAP-8294A2 in a member of <i<L. capsici</i< species. Moreover, UHPLC-HRMS/MS confirmed the presence of dihydromaltophilin/Heat Stable Antifungal Factor (HSAF) in AZ78 cell-free extracts. Due to the production of HSAF by AZ78, cell-free supernatants were effective in controlling <i<Plasmopara viticola</i< on grapevine leaf disks after exposure to high temperatures. Overall, our work determined the main secondary metabolites involved in the biocontrol activity of AZ78 against plant pathogenic oomycetes and Gram-positive bacteria. These results might be useful for the future development of this bacterial strain as the active ingredient of a microbial biopesticide that might contribute to a reduction in the chemical input in agriculture. <i<Lysobacter capsici</i< WAP-8294A2 dihydromaltophilin (HSAF) 2,5-diketopiperazines MALDI-qTOF-MSI UHPLC-HRMS/MS Biology (General) Anthi Vlassi verfasserin aut Ana Bejarano verfasserin aut Bernard Seidl verfasserin aut Martina Marchetti-Deschmann verfasserin aut Rainer Schuhmacher verfasserin aut Gerardo Puopolo verfasserin aut In Microorganisms MDPI AG, 2013 9(2021), 6, p 1320 (DE-627)750370696 (DE-600)2720891-6 20762607 nnns volume:9 year:2021 number:6, p 1320 https://doi.org/10.3390/microorganisms9061320 kostenfrei https://doaj.org/article/ba99b35b77b443c48b313e944b4f41ec kostenfrei https://www.mdpi.com/2076-2607/9/6/1320 kostenfrei https://doaj.org/toc/2076-2607 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 6, p 1320
language	English
source	In Microorganisms 9(2021), 6, p 1320 volume:9 year:2021 number:6, p 1320
sourceStr	In Microorganisms 9(2021), 6, p 1320 volume:9 year:2021 number:6, p 1320
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	<i<Lysobacter capsici</i< WAP-8294A2 dihydromaltophilin (HSAF) 2,5-diketopiperazines MALDI-qTOF-MSI UHPLC-HRMS/MS Biology (General)
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container_title	Microorganisms
authorswithroles_txt_mv	Francesca Brescia @@aut@@ Anthi Vlassi @@aut@@ Ana Bejarano @@aut@@ Bernard Seidl @@aut@@ Martina Marchetti-Deschmann @@aut@@ Rainer Schuhmacher @@aut@@ Gerardo Puopolo @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	750370696
id	DOAJ052856402
language_de	englisch
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callnumber-first	Q - Science
author	Francesca Brescia
spellingShingle	Francesca Brescia misc QH301-705.5 misc <i<Lysobacter capsici</i< misc WAP-8294A2 misc dihydromaltophilin (HSAF) misc 2,5-diketopiperazines misc MALDI-qTOF-MSI misc UHPLC-HRMS/MS misc Biology (General) Characterisation of the Antibiotic Profile of <i<Lysobacter capsici</i< AZ78, an Effective Biological Control Agent of Plant Pathogenic Microorganisms
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topic_title	QH301-705.5 Characterisation of the Antibiotic Profile of <i<Lysobacter capsici</i< AZ78, an Effective Biological Control Agent of Plant Pathogenic Microorganisms <i<Lysobacter capsici</i< WAP-8294A2 dihydromaltophilin (HSAF) 2,5-diketopiperazines MALDI-qTOF-MSI UHPLC-HRMS/MS
topic	misc QH301-705.5 misc <i<Lysobacter capsici</i< misc WAP-8294A2 misc dihydromaltophilin (HSAF) misc 2,5-diketopiperazines misc MALDI-qTOF-MSI misc UHPLC-HRMS/MS misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc <i<Lysobacter capsici</i< misc WAP-8294A2 misc dihydromaltophilin (HSAF) misc 2,5-diketopiperazines misc MALDI-qTOF-MSI misc UHPLC-HRMS/MS misc Biology (General)
topic_browse	misc QH301-705.5 misc <i<Lysobacter capsici</i< misc WAP-8294A2 misc dihydromaltophilin (HSAF) misc 2,5-diketopiperazines misc MALDI-qTOF-MSI misc UHPLC-HRMS/MS misc Biology (General)
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title	Characterisation of the Antibiotic Profile of <i<Lysobacter capsici</i< AZ78, an Effective Biological Control Agent of Plant Pathogenic Microorganisms
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title_full	Characterisation of the Antibiotic Profile of <i<Lysobacter capsici</i< AZ78, an Effective Biological Control Agent of Plant Pathogenic Microorganisms
author_sort	Francesca Brescia
journal	Microorganisms
journalStr	Microorganisms
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author_browse	Francesca Brescia Anthi Vlassi Ana Bejarano Bernard Seidl Martina Marchetti-Deschmann Rainer Schuhmacher Gerardo Puopolo
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author-letter	Francesca Brescia
doi_str_mv	10.3390/microorganisms9061320
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title_sort	characterisation of the antibiotic profile of <i<lysobacter capsici</i< az78, an effective biological control agent of plant pathogenic microorganisms
callnumber	QH301-705.5
title_auth	Characterisation of the Antibiotic Profile of <i<Lysobacter capsici</i< AZ78, an Effective Biological Control Agent of Plant Pathogenic Microorganisms
abstract	Determining the mode of action of microbial biocontrol agents plays a key role in their development and registration as commercial biopesticides. The biocontrol rhizobacterium <i<Lysobacter capsici</i< AZ78 (AZ78) is able to inhibit a vast array of plant pathogenic oomycetes and Gram-positive bacteria due to the release of antimicrobial secondary metabolites. A combination of MALDI-qTOF-MSI and UHPLC-HRMS/M was applied to finely dissect the AZ78 metabolome and identify the main secondary metabolites involved in the inhibition of plant pathogenic microorganisms. Under nutritionally limited conditions, MALDI-qTOF-MSI revealed that AZ78 is able to release a relevant number of antimicrobial secondary metabolites belonging to the families of 2,5-diketopiperazines, cyclic lipodepsipeptides, macrolactones and macrolides. In vitro tests confirmed the presence of secondary metabolites toxic against <i<Pythium ultimum</i< and <i<Rhodococcus fascians</i< in AZ78 cell-free extracts. Subsequently, UHPLC-HRMS/MS was used to confirm the results achieved with MALDI-qTOF-MSI and investigate for further putative antimicrobial secondary metabolites known to be produced by <i<Lysobacter</i< spp. This technique confirmed the presence of several 2,5-diketopiperazines in AZ78 cell-free extracts and provided the first evidence of the production of the cyclic depsipeptide WAP-8294A2 in a member of <i<L. capsici</i< species. Moreover, UHPLC-HRMS/MS confirmed the presence of dihydromaltophilin/Heat Stable Antifungal Factor (HSAF) in AZ78 cell-free extracts. Due to the production of HSAF by AZ78, cell-free supernatants were effective in controlling <i<Plasmopara viticola</i< on grapevine leaf disks after exposure to high temperatures. Overall, our work determined the main secondary metabolites involved in the biocontrol activity of AZ78 against plant pathogenic oomycetes and Gram-positive bacteria. These results might be useful for the future development of this bacterial strain as the active ingredient of a microbial biopesticide that might contribute to a reduction in the chemical input in agriculture.
abstractGer	Determining the mode of action of microbial biocontrol agents plays a key role in their development and registration as commercial biopesticides. The biocontrol rhizobacterium <i<Lysobacter capsici</i< AZ78 (AZ78) is able to inhibit a vast array of plant pathogenic oomycetes and Gram-positive bacteria due to the release of antimicrobial secondary metabolites. A combination of MALDI-qTOF-MSI and UHPLC-HRMS/M was applied to finely dissect the AZ78 metabolome and identify the main secondary metabolites involved in the inhibition of plant pathogenic microorganisms. Under nutritionally limited conditions, MALDI-qTOF-MSI revealed that AZ78 is able to release a relevant number of antimicrobial secondary metabolites belonging to the families of 2,5-diketopiperazines, cyclic lipodepsipeptides, macrolactones and macrolides. In vitro tests confirmed the presence of secondary metabolites toxic against <i<Pythium ultimum</i< and <i<Rhodococcus fascians</i< in AZ78 cell-free extracts. Subsequently, UHPLC-HRMS/MS was used to confirm the results achieved with MALDI-qTOF-MSI and investigate for further putative antimicrobial secondary metabolites known to be produced by <i<Lysobacter</i< spp. This technique confirmed the presence of several 2,5-diketopiperazines in AZ78 cell-free extracts and provided the first evidence of the production of the cyclic depsipeptide WAP-8294A2 in a member of <i<L. capsici</i< species. Moreover, UHPLC-HRMS/MS confirmed the presence of dihydromaltophilin/Heat Stable Antifungal Factor (HSAF) in AZ78 cell-free extracts. Due to the production of HSAF by AZ78, cell-free supernatants were effective in controlling <i<Plasmopara viticola</i< on grapevine leaf disks after exposure to high temperatures. Overall, our work determined the main secondary metabolites involved in the biocontrol activity of AZ78 against plant pathogenic oomycetes and Gram-positive bacteria. These results might be useful for the future development of this bacterial strain as the active ingredient of a microbial biopesticide that might contribute to a reduction in the chemical input in agriculture.
abstract_unstemmed	Determining the mode of action of microbial biocontrol agents plays a key role in their development and registration as commercial biopesticides. The biocontrol rhizobacterium <i<Lysobacter capsici</i< AZ78 (AZ78) is able to inhibit a vast array of plant pathogenic oomycetes and Gram-positive bacteria due to the release of antimicrobial secondary metabolites. A combination of MALDI-qTOF-MSI and UHPLC-HRMS/M was applied to finely dissect the AZ78 metabolome and identify the main secondary metabolites involved in the inhibition of plant pathogenic microorganisms. Under nutritionally limited conditions, MALDI-qTOF-MSI revealed that AZ78 is able to release a relevant number of antimicrobial secondary metabolites belonging to the families of 2,5-diketopiperazines, cyclic lipodepsipeptides, macrolactones and macrolides. In vitro tests confirmed the presence of secondary metabolites toxic against <i<Pythium ultimum</i< and <i<Rhodococcus fascians</i< in AZ78 cell-free extracts. Subsequently, UHPLC-HRMS/MS was used to confirm the results achieved with MALDI-qTOF-MSI and investigate for further putative antimicrobial secondary metabolites known to be produced by <i<Lysobacter</i< spp. This technique confirmed the presence of several 2,5-diketopiperazines in AZ78 cell-free extracts and provided the first evidence of the production of the cyclic depsipeptide WAP-8294A2 in a member of <i<L. capsici</i< species. Moreover, UHPLC-HRMS/MS confirmed the presence of dihydromaltophilin/Heat Stable Antifungal Factor (HSAF) in AZ78 cell-free extracts. Due to the production of HSAF by AZ78, cell-free supernatants were effective in controlling <i<Plasmopara viticola</i< on grapevine leaf disks after exposure to high temperatures. Overall, our work determined the main secondary metabolites involved in the biocontrol activity of AZ78 against plant pathogenic oomycetes and Gram-positive bacteria. These results might be useful for the future development of this bacterial strain as the active ingredient of a microbial biopesticide that might contribute to a reduction in the chemical input in agriculture.
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container_issue	6, p 1320
title_short	Characterisation of the Antibiotic Profile of <i<Lysobacter capsici</i< AZ78, an Effective Biological Control Agent of Plant Pathogenic Microorganisms
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author2	Anthi Vlassi Ana Bejarano Bernard Seidl Martina Marchetti-Deschmann Rainer Schuhmacher Gerardo Puopolo
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