Epidemic IncX3 plasmids spreading carbapenemase genes in the United Arab Emirates and worldwide

Shaimaa F Mouftah,1,* Tibor Pál,1,* Dania Darwish,1 Akela Ghazawi,1 Laura Villa,2 Alessandra Carattoli,3 Ágnes Sonnevend1,41Department of Medical Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates; 2Department of Infectious Diseases, Istituto Superiore di Sanitá, Rome, Italy; 3Department of Molecular Microbiology, University of Rome La Sapienza, Rome, Italy; 4Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Pécs, Hungary*These authors contributed equally to this work Purpose: Plasmids of the incompatibility group X type 3 (IncX3) were described carrying various carbapenemase genes in carbapenemase-producing Enterobacteriaceae (CPE) worldwide and in the United Arab Emirates (UAE), as well. To understand the driving force behind the emergence of such plasmids in the UAE, the relationship between IncX3 plasmids encountered locally and globally was investigated.Methods: CPE strains isolated in the UAE during 2009–2014 were screened by X3 PCR-based replicon typing. The clonal relationship of CPE carrying IncX3 plasmids was determined by multi-locus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). Complete sequence of selected IncX3 plasmids was determined. Phylogenetic relationship between the carbapenemase carrying IncX3 plasmids from the UAE and of those reported worldwide was established by comparing the plasmid backbones.Results: 10.2% of the 295 CPE tested were identified to carry IncX3 plasmids: 13 Escherichia coli, 13 Klebsiella pneumoniae, two Enterobacter cloacae, one Citrobacter freundii and one Morganella morganii isolate, respectively. Most of them were non-clonal; with small clusters of triplets and pairs of E. coli and K. pneumoniae, and a cluster of five K. pneumoniae ST11 exhibiting >90% similar PFGE patterns, respectively. The 30 isolates harbored either blaNDM-1, blaNDM-4, blaNDM-5, blaNDM-7, blaOXA-181 or blaKPC-2 carbapenemase genes on IncX3 plasmids. Phylogenetic analysis of the backbone region of IncX3 plasmids carrying various beta-lactamase genes from the UAE (n=23) and that of North-America, Europe, Asia and Australia (n=35) revealed three clusters based on the carbapenemase genes carried: plasmids harboring blaOXA-181 and blaNDM-5 formed two distinct groups, whereas backbones of plasmids with blaNDM-1, blaNDM-4 and blaNDM-7 clustered together. Each cluster contained plasmids of diverse geographical origin.Conclusion: The findings suggest that different carbapenemase gene carrying IncX3 plasmids encountered in the UAE do not evolve locally, rather are subtypes of this epidemic plasmid emerging in this country due to international transfer.Keywords: Enterobacterales, carbapenemase genes, IncX3 plasmid, Middle-East Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Mouftah SF [verfasserIn] Pál T [verfasserIn] Darwish D [verfasserIn] Ghazawi A [verfasserIn] Villa L [verfasserIn] Carattoli A [verfasserIn] Sonnevend Á [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	Enterobacteriaceae carbapenemase genes IncX3 plasmid Middle-East

Übergeordnetes Werk:	In: Infection and Drug Resistance - Dove Medical Press, 2009, (2019), Seite 1729-1742
Übergeordnetes Werk:	year:2019 ; pages:1729-1742

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Katalog-ID:	DOAJ052983609

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520			\|a Shaimaa F Mouftah,1,* Tibor Pál,1,* Dania Darwish,1 Akela Ghazawi,1 Laura Villa,2 Alessandra Carattoli,3 Ágnes Sonnevend1,41Department of Medical Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates; 2Department of Infectious Diseases, Istituto Superiore di Sanitá, Rome, Italy; 3Department of Molecular Microbiology, University of Rome La Sapienza, Rome, Italy; 4Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Pécs, Hungary*These authors contributed equally to this work Purpose: Plasmids of the incompatibility group X type 3 (IncX3) were described carrying various carbapenemase genes in carbapenemase-producing Enterobacteriaceae (CPE) worldwide and in the United Arab Emirates (UAE), as well. To understand the driving force behind the emergence of such plasmids in the UAE, the relationship between IncX3 plasmids encountered locally and globally was investigated.Methods: CPE strains isolated in the UAE during 2009–2014 were screened by X3 PCR-based replicon typing. The clonal relationship of CPE carrying IncX3 plasmids was determined by multi-locus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). Complete sequence of selected IncX3 plasmids was determined. Phylogenetic relationship between the carbapenemase carrying IncX3 plasmids from the UAE and of those reported worldwide was established by comparing the plasmid backbones.Results: 10.2% of the 295 CPE tested were identified to carry IncX3 plasmids: 13 Escherichia coli, 13 Klebsiella pneumoniae, two Enterobacter cloacae, one Citrobacter freundii and one Morganella morganii isolate, respectively. Most of them were non-clonal; with small clusters of triplets and pairs of E. coli and K. pneumoniae, and a cluster of five K. pneumoniae ST11 exhibiting >90% similar PFGE patterns, respectively. The 30 isolates harbored either blaNDM-1, blaNDM-4, blaNDM-5, blaNDM-7, blaOXA-181 or blaKPC-2 carbapenemase genes on IncX3 plasmids. Phylogenetic analysis of the backbone region of IncX3 plasmids carrying various beta-lactamase genes from the UAE (n=23) and that of North-America, Europe, Asia and Australia (n=35) revealed three clusters based on the carbapenemase genes carried: plasmids harboring blaOXA-181 and blaNDM-5 formed two distinct groups, whereas backbones of plasmids with blaNDM-1, blaNDM-4 and blaNDM-7 clustered together. Each cluster contained plasmids of diverse geographical origin.Conclusion: The findings suggest that different carbapenemase gene carrying IncX3 plasmids encountered in the UAE do not evolve locally, rather are subtypes of this epidemic plasmid emerging in this country due to international transfer.Keywords: Enterobacterales, carbapenemase genes, IncX3 plasmid, Middle-East
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allfields	(DE-627)DOAJ052983609 (DE-599)DOAJd7a353e2e8844833b83c96464455020f DE-627 ger DE-627 rakwb eng RC109-216 Mouftah SF verfasserin aut Epidemic IncX3 plasmids spreading carbapenemase genes in the United Arab Emirates and worldwide 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Shaimaa F Mouftah,1,* Tibor Pál,1,* Dania Darwish,1 Akela Ghazawi,1 Laura Villa,2 Alessandra Carattoli,3 Ágnes Sonnevend1,41Department of Medical Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates; 2Department of Infectious Diseases, Istituto Superiore di Sanitá, Rome, Italy; 3Department of Molecular Microbiology, University of Rome La Sapienza, Rome, Italy; 4Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Pécs, Hungary*These authors contributed equally to this work Purpose: Plasmids of the incompatibility group X type 3 (IncX3) were described carrying various carbapenemase genes in carbapenemase-producing Enterobacteriaceae (CPE) worldwide and in the United Arab Emirates (UAE), as well. To understand the driving force behind the emergence of such plasmids in the UAE, the relationship between IncX3 plasmids encountered locally and globally was investigated.Methods: CPE strains isolated in the UAE during 2009–2014 were screened by X3 PCR-based replicon typing. The clonal relationship of CPE carrying IncX3 plasmids was determined by multi-locus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). Complete sequence of selected IncX3 plasmids was determined. Phylogenetic relationship between the carbapenemase carrying IncX3 plasmids from the UAE and of those reported worldwide was established by comparing the plasmid backbones.Results: 10.2% of the 295 CPE tested were identified to carry IncX3 plasmids: 13 Escherichia coli, 13 Klebsiella pneumoniae, two Enterobacter cloacae, one Citrobacter freundii and one Morganella morganii isolate, respectively. Most of them were non-clonal; with small clusters of triplets and pairs of E. coli and K. pneumoniae, and a cluster of five K. pneumoniae ST11 exhibiting >90% similar PFGE patterns, respectively. The 30 isolates harbored either blaNDM-1, blaNDM-4, blaNDM-5, blaNDM-7, blaOXA-181 or blaKPC-2 carbapenemase genes on IncX3 plasmids. Phylogenetic analysis of the backbone region of IncX3 plasmids carrying various beta-lactamase genes from the UAE (n=23) and that of North-America, Europe, Asia and Australia (n=35) revealed three clusters based on the carbapenemase genes carried: plasmids harboring blaOXA-181 and blaNDM-5 formed two distinct groups, whereas backbones of plasmids with blaNDM-1, blaNDM-4 and blaNDM-7 clustered together. Each cluster contained plasmids of diverse geographical origin.Conclusion: The findings suggest that different carbapenemase gene carrying IncX3 plasmids encountered in the UAE do not evolve locally, rather are subtypes of this epidemic plasmid emerging in this country due to international transfer.Keywords: Enterobacterales, carbapenemase genes, IncX3 plasmid, Middle-East Enterobacteriaceae carbapenemase genes IncX3 plasmid Middle-East Infectious and parasitic diseases Pál T verfasserin aut Darwish D verfasserin aut Ghazawi A verfasserin aut Villa L verfasserin aut Carattoli A verfasserin aut Sonnevend Á verfasserin aut In Infection and Drug Resistance Dove Medical Press, 2009 (2019), Seite 1729-1742 (DE-627)600305996 (DE-600)2494856-1 11786973 nnns year:2019 pages:1729-1742 https://doaj.org/article/d7a353e2e8844833b83c96464455020f kostenfrei https://www.dovepress.com/epidemic-incx3-plasmids-spreading-carbapenemase-genes-in-the-united-ar-peer-reviewed-article-IDR kostenfrei https://doaj.org/toc/1178-6973 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2019 1729-1742
spelling	(DE-627)DOAJ052983609 (DE-599)DOAJd7a353e2e8844833b83c96464455020f DE-627 ger DE-627 rakwb eng RC109-216 Mouftah SF verfasserin aut Epidemic IncX3 plasmids spreading carbapenemase genes in the United Arab Emirates and worldwide 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Shaimaa F Mouftah,1,* Tibor Pál,1,* Dania Darwish,1 Akela Ghazawi,1 Laura Villa,2 Alessandra Carattoli,3 Ágnes Sonnevend1,41Department of Medical Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates; 2Department of Infectious Diseases, Istituto Superiore di Sanitá, Rome, Italy; 3Department of Molecular Microbiology, University of Rome La Sapienza, Rome, Italy; 4Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Pécs, Hungary*These authors contributed equally to this work Purpose: Plasmids of the incompatibility group X type 3 (IncX3) were described carrying various carbapenemase genes in carbapenemase-producing Enterobacteriaceae (CPE) worldwide and in the United Arab Emirates (UAE), as well. To understand the driving force behind the emergence of such plasmids in the UAE, the relationship between IncX3 plasmids encountered locally and globally was investigated.Methods: CPE strains isolated in the UAE during 2009–2014 were screened by X3 PCR-based replicon typing. The clonal relationship of CPE carrying IncX3 plasmids was determined by multi-locus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). Complete sequence of selected IncX3 plasmids was determined. Phylogenetic relationship between the carbapenemase carrying IncX3 plasmids from the UAE and of those reported worldwide was established by comparing the plasmid backbones.Results: 10.2% of the 295 CPE tested were identified to carry IncX3 plasmids: 13 Escherichia coli, 13 Klebsiella pneumoniae, two Enterobacter cloacae, one Citrobacter freundii and one Morganella morganii isolate, respectively. Most of them were non-clonal; with small clusters of triplets and pairs of E. coli and K. pneumoniae, and a cluster of five K. pneumoniae ST11 exhibiting >90% similar PFGE patterns, respectively. The 30 isolates harbored either blaNDM-1, blaNDM-4, blaNDM-5, blaNDM-7, blaOXA-181 or blaKPC-2 carbapenemase genes on IncX3 plasmids. Phylogenetic analysis of the backbone region of IncX3 plasmids carrying various beta-lactamase genes from the UAE (n=23) and that of North-America, Europe, Asia and Australia (n=35) revealed three clusters based on the carbapenemase genes carried: plasmids harboring blaOXA-181 and blaNDM-5 formed two distinct groups, whereas backbones of plasmids with blaNDM-1, blaNDM-4 and blaNDM-7 clustered together. Each cluster contained plasmids of diverse geographical origin.Conclusion: The findings suggest that different carbapenemase gene carrying IncX3 plasmids encountered in the UAE do not evolve locally, rather are subtypes of this epidemic plasmid emerging in this country due to international transfer.Keywords: Enterobacterales, carbapenemase genes, IncX3 plasmid, Middle-East Enterobacteriaceae carbapenemase genes IncX3 plasmid Middle-East Infectious and parasitic diseases Pál T verfasserin aut Darwish D verfasserin aut Ghazawi A verfasserin aut Villa L verfasserin aut Carattoli A verfasserin aut Sonnevend Á verfasserin aut In Infection and Drug Resistance Dove Medical Press, 2009 (2019), Seite 1729-1742 (DE-627)600305996 (DE-600)2494856-1 11786973 nnns year:2019 pages:1729-1742 https://doaj.org/article/d7a353e2e8844833b83c96464455020f kostenfrei https://www.dovepress.com/epidemic-incx3-plasmids-spreading-carbapenemase-genes-in-the-united-ar-peer-reviewed-article-IDR kostenfrei https://doaj.org/toc/1178-6973 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2019 1729-1742
allfields_unstemmed	(DE-627)DOAJ052983609 (DE-599)DOAJd7a353e2e8844833b83c96464455020f DE-627 ger DE-627 rakwb eng RC109-216 Mouftah SF verfasserin aut Epidemic IncX3 plasmids spreading carbapenemase genes in the United Arab Emirates and worldwide 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Shaimaa F Mouftah,1,* Tibor Pál,1,* Dania Darwish,1 Akela Ghazawi,1 Laura Villa,2 Alessandra Carattoli,3 Ágnes Sonnevend1,41Department of Medical Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates; 2Department of Infectious Diseases, Istituto Superiore di Sanitá, Rome, Italy; 3Department of Molecular Microbiology, University of Rome La Sapienza, Rome, Italy; 4Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Pécs, Hungary*These authors contributed equally to this work Purpose: Plasmids of the incompatibility group X type 3 (IncX3) were described carrying various carbapenemase genes in carbapenemase-producing Enterobacteriaceae (CPE) worldwide and in the United Arab Emirates (UAE), as well. To understand the driving force behind the emergence of such plasmids in the UAE, the relationship between IncX3 plasmids encountered locally and globally was investigated.Methods: CPE strains isolated in the UAE during 2009–2014 were screened by X3 PCR-based replicon typing. The clonal relationship of CPE carrying IncX3 plasmids was determined by multi-locus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). Complete sequence of selected IncX3 plasmids was determined. Phylogenetic relationship between the carbapenemase carrying IncX3 plasmids from the UAE and of those reported worldwide was established by comparing the plasmid backbones.Results: 10.2% of the 295 CPE tested were identified to carry IncX3 plasmids: 13 Escherichia coli, 13 Klebsiella pneumoniae, two Enterobacter cloacae, one Citrobacter freundii and one Morganella morganii isolate, respectively. Most of them were non-clonal; with small clusters of triplets and pairs of E. coli and K. pneumoniae, and a cluster of five K. pneumoniae ST11 exhibiting >90% similar PFGE patterns, respectively. The 30 isolates harbored either blaNDM-1, blaNDM-4, blaNDM-5, blaNDM-7, blaOXA-181 or blaKPC-2 carbapenemase genes on IncX3 plasmids. Phylogenetic analysis of the backbone region of IncX3 plasmids carrying various beta-lactamase genes from the UAE (n=23) and that of North-America, Europe, Asia and Australia (n=35) revealed three clusters based on the carbapenemase genes carried: plasmids harboring blaOXA-181 and blaNDM-5 formed two distinct groups, whereas backbones of plasmids with blaNDM-1, blaNDM-4 and blaNDM-7 clustered together. Each cluster contained plasmids of diverse geographical origin.Conclusion: The findings suggest that different carbapenemase gene carrying IncX3 plasmids encountered in the UAE do not evolve locally, rather are subtypes of this epidemic plasmid emerging in this country due to international transfer.Keywords: Enterobacterales, carbapenemase genes, IncX3 plasmid, Middle-East Enterobacteriaceae carbapenemase genes IncX3 plasmid Middle-East Infectious and parasitic diseases Pál T verfasserin aut Darwish D verfasserin aut Ghazawi A verfasserin aut Villa L verfasserin aut Carattoli A verfasserin aut Sonnevend Á verfasserin aut In Infection and Drug Resistance Dove Medical Press, 2009 (2019), Seite 1729-1742 (DE-627)600305996 (DE-600)2494856-1 11786973 nnns year:2019 pages:1729-1742 https://doaj.org/article/d7a353e2e8844833b83c96464455020f kostenfrei https://www.dovepress.com/epidemic-incx3-plasmids-spreading-carbapenemase-genes-in-the-united-ar-peer-reviewed-article-IDR kostenfrei https://doaj.org/toc/1178-6973 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2019 1729-1742
allfieldsGer	(DE-627)DOAJ052983609 (DE-599)DOAJd7a353e2e8844833b83c96464455020f DE-627 ger DE-627 rakwb eng RC109-216 Mouftah SF verfasserin aut Epidemic IncX3 plasmids spreading carbapenemase genes in the United Arab Emirates and worldwide 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Shaimaa F Mouftah,1,* Tibor Pál,1,* Dania Darwish,1 Akela Ghazawi,1 Laura Villa,2 Alessandra Carattoli,3 Ágnes Sonnevend1,41Department of Medical Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates; 2Department of Infectious Diseases, Istituto Superiore di Sanitá, Rome, Italy; 3Department of Molecular Microbiology, University of Rome La Sapienza, Rome, Italy; 4Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Pécs, Hungary*These authors contributed equally to this work Purpose: Plasmids of the incompatibility group X type 3 (IncX3) were described carrying various carbapenemase genes in carbapenemase-producing Enterobacteriaceae (CPE) worldwide and in the United Arab Emirates (UAE), as well. To understand the driving force behind the emergence of such plasmids in the UAE, the relationship between IncX3 plasmids encountered locally and globally was investigated.Methods: CPE strains isolated in the UAE during 2009–2014 were screened by X3 PCR-based replicon typing. The clonal relationship of CPE carrying IncX3 plasmids was determined by multi-locus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). Complete sequence of selected IncX3 plasmids was determined. Phylogenetic relationship between the carbapenemase carrying IncX3 plasmids from the UAE and of those reported worldwide was established by comparing the plasmid backbones.Results: 10.2% of the 295 CPE tested were identified to carry IncX3 plasmids: 13 Escherichia coli, 13 Klebsiella pneumoniae, two Enterobacter cloacae, one Citrobacter freundii and one Morganella morganii isolate, respectively. Most of them were non-clonal; with small clusters of triplets and pairs of E. coli and K. pneumoniae, and a cluster of five K. pneumoniae ST11 exhibiting >90% similar PFGE patterns, respectively. The 30 isolates harbored either blaNDM-1, blaNDM-4, blaNDM-5, blaNDM-7, blaOXA-181 or blaKPC-2 carbapenemase genes on IncX3 plasmids. Phylogenetic analysis of the backbone region of IncX3 plasmids carrying various beta-lactamase genes from the UAE (n=23) and that of North-America, Europe, Asia and Australia (n=35) revealed three clusters based on the carbapenemase genes carried: plasmids harboring blaOXA-181 and blaNDM-5 formed two distinct groups, whereas backbones of plasmids with blaNDM-1, blaNDM-4 and blaNDM-7 clustered together. Each cluster contained plasmids of diverse geographical origin.Conclusion: The findings suggest that different carbapenemase gene carrying IncX3 plasmids encountered in the UAE do not evolve locally, rather are subtypes of this epidemic plasmid emerging in this country due to international transfer.Keywords: Enterobacterales, carbapenemase genes, IncX3 plasmid, Middle-East Enterobacteriaceae carbapenemase genes IncX3 plasmid Middle-East Infectious and parasitic diseases Pál T verfasserin aut Darwish D verfasserin aut Ghazawi A verfasserin aut Villa L verfasserin aut Carattoli A verfasserin aut Sonnevend Á verfasserin aut In Infection and Drug Resistance Dove Medical Press, 2009 (2019), Seite 1729-1742 (DE-627)600305996 (DE-600)2494856-1 11786973 nnns year:2019 pages:1729-1742 https://doaj.org/article/d7a353e2e8844833b83c96464455020f kostenfrei https://www.dovepress.com/epidemic-incx3-plasmids-spreading-carbapenemase-genes-in-the-united-ar-peer-reviewed-article-IDR kostenfrei https://doaj.org/toc/1178-6973 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2019 1729-1742
allfieldsSound	(DE-627)DOAJ052983609 (DE-599)DOAJd7a353e2e8844833b83c96464455020f DE-627 ger DE-627 rakwb eng RC109-216 Mouftah SF verfasserin aut Epidemic IncX3 plasmids spreading carbapenemase genes in the United Arab Emirates and worldwide 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Shaimaa F Mouftah,1,* Tibor Pál,1,* Dania Darwish,1 Akela Ghazawi,1 Laura Villa,2 Alessandra Carattoli,3 Ágnes Sonnevend1,41Department of Medical Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates; 2Department of Infectious Diseases, Istituto Superiore di Sanitá, Rome, Italy; 3Department of Molecular Microbiology, University of Rome La Sapienza, Rome, Italy; 4Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Pécs, Hungary*These authors contributed equally to this work Purpose: Plasmids of the incompatibility group X type 3 (IncX3) were described carrying various carbapenemase genes in carbapenemase-producing Enterobacteriaceae (CPE) worldwide and in the United Arab Emirates (UAE), as well. To understand the driving force behind the emergence of such plasmids in the UAE, the relationship between IncX3 plasmids encountered locally and globally was investigated.Methods: CPE strains isolated in the UAE during 2009–2014 were screened by X3 PCR-based replicon typing. The clonal relationship of CPE carrying IncX3 plasmids was determined by multi-locus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). Complete sequence of selected IncX3 plasmids was determined. Phylogenetic relationship between the carbapenemase carrying IncX3 plasmids from the UAE and of those reported worldwide was established by comparing the plasmid backbones.Results: 10.2% of the 295 CPE tested were identified to carry IncX3 plasmids: 13 Escherichia coli, 13 Klebsiella pneumoniae, two Enterobacter cloacae, one Citrobacter freundii and one Morganella morganii isolate, respectively. Most of them were non-clonal; with small clusters of triplets and pairs of E. coli and K. pneumoniae, and a cluster of five K. pneumoniae ST11 exhibiting >90% similar PFGE patterns, respectively. The 30 isolates harbored either blaNDM-1, blaNDM-4, blaNDM-5, blaNDM-7, blaOXA-181 or blaKPC-2 carbapenemase genes on IncX3 plasmids. Phylogenetic analysis of the backbone region of IncX3 plasmids carrying various beta-lactamase genes from the UAE (n=23) and that of North-America, Europe, Asia and Australia (n=35) revealed three clusters based on the carbapenemase genes carried: plasmids harboring blaOXA-181 and blaNDM-5 formed two distinct groups, whereas backbones of plasmids with blaNDM-1, blaNDM-4 and blaNDM-7 clustered together. Each cluster contained plasmids of diverse geographical origin.Conclusion: The findings suggest that different carbapenemase gene carrying IncX3 plasmids encountered in the UAE do not evolve locally, rather are subtypes of this epidemic plasmid emerging in this country due to international transfer.Keywords: Enterobacterales, carbapenemase genes, IncX3 plasmid, Middle-East Enterobacteriaceae carbapenemase genes IncX3 plasmid Middle-East Infectious and parasitic diseases Pál T verfasserin aut Darwish D verfasserin aut Ghazawi A verfasserin aut Villa L verfasserin aut Carattoli A verfasserin aut Sonnevend Á verfasserin aut In Infection and Drug Resistance Dove Medical Press, 2009 (2019), Seite 1729-1742 (DE-627)600305996 (DE-600)2494856-1 11786973 nnns year:2019 pages:1729-1742 https://doaj.org/article/d7a353e2e8844833b83c96464455020f kostenfrei https://www.dovepress.com/epidemic-incx3-plasmids-spreading-carbapenemase-genes-in-the-united-ar-peer-reviewed-article-IDR kostenfrei https://doaj.org/toc/1178-6973 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2019 1729-1742
language	English
source	In Infection and Drug Resistance (2019), Seite 1729-1742 year:2019 pages:1729-1742
sourceStr	In Infection and Drug Resistance (2019), Seite 1729-1742 year:2019 pages:1729-1742
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Enterobacteriaceae carbapenemase genes IncX3 plasmid Middle-East Infectious and parasitic diseases
isfreeaccess_bool	true
container_title	Infection and Drug Resistance
authorswithroles_txt_mv	Mouftah SF @@aut@@ Pál T @@aut@@ Darwish D @@aut@@ Ghazawi A @@aut@@ Villa L @@aut@@ Carattoli A @@aut@@ Sonnevend Á @@aut@@
publishDateDaySort_date	2019-01-01T00:00:00Z
hierarchy_top_id	600305996
id	DOAJ052983609
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ052983609</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230308171834.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230227s2019 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ052983609</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJd7a353e2e8844833b83c96464455020f</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">RC109-216</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Mouftah SF</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Epidemic IncX3 plasmids spreading carbapenemase genes in the United Arab Emirates and worldwide</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2019</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Shaimaa F Mouftah,1,* Tibor P&aacute;l,1,* Dania Darwish,1 Akela Ghazawi,1 Laura Villa,2 Alessandra Carattoli,3 &Aacute;gnes Sonnevend1,41Department of Medical Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates; 2Department of Infectious Diseases, Istituto Superiore di Sanit&aacute;, Rome, Italy; 3Department of Molecular Microbiology, University of Rome La Sapienza, Rome, Italy; 4Department of Medical Microbiology and Immunology, Medical School, University of P&eacute;cs, P&eacute;cs, Hungary*These authors contributed equally to this work Purpose: Plasmids of the incompatibility group X type 3 (IncX3) were described carrying various carbapenemase genes in carbapenemase-producing Enterobacteriaceae (CPE) worldwide and in the United Arab Emirates (UAE), as well. To understand the driving force behind the emergence of such plasmids in the UAE, the relationship between IncX3 plasmids encountered locally and globally was investigated.Methods: CPE strains isolated in the UAE during 2009&ndash;2014 were screened by X3 PCR-based replicon typing. The clonal relationship of CPE carrying IncX3 plasmids was determined by multi-locus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). Complete sequence of selected IncX3 plasmids was determined. Phylogenetic relationship between the carbapenemase carrying IncX3 plasmids from the UAE and of those reported worldwide was established by comparing the plasmid backbones.Results: 10.2% of the 295 CPE tested were identified to carry IncX3 plasmids: 13 Escherichia coli, 13 Klebsiella pneumoniae, two Enterobacter cloacae, one Citrobacter freundii and one Morganella morganii isolate, respectively. Most of them were non-clonal; with small clusters of triplets and pairs of E. coli and K. pneumoniae, and a cluster of five K. pneumoniae ST11 exhibiting &gt;90% similar PFGE patterns, respectively. The 30 isolates harbored either blaNDM-1, blaNDM-4, blaNDM-5, blaNDM-7, blaOXA-181 or blaKPC-2 carbapenemase genes on IncX3 plasmids. Phylogenetic analysis of the backbone region of IncX3 plasmids carrying various beta-lactamase genes from the UAE (n=23) and that of North-America, Europe, Asia and Australia (n=35) revealed three clusters based on the carbapenemase genes carried: plasmids harboring blaOXA-181 and blaNDM-5 formed two distinct groups, whereas backbones of plasmids with blaNDM-1, blaNDM-4 and blaNDM-7 clustered together. Each cluster contained plasmids of diverse geographical origin.Conclusion: The findings suggest that different carbapenemase gene carrying IncX3 plasmids encountered in the UAE do not evolve locally, rather are subtypes of this epidemic plasmid emerging in this country due to international transfer.Keywords: Enterobacterales, carbapenemase genes, IncX3 plasmid, Middle-East</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Enterobacteriaceae</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">carbapenemase genes</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">IncX3 plasmid</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Middle-East</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Infectious and parasitic diseases</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Pál T</subfield><subfield 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callnumber-first	R - Medicine
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spellingShingle	Mouftah SF misc RC109-216 misc Enterobacteriaceae misc carbapenemase genes misc IncX3 plasmid misc Middle-East misc Infectious and parasitic diseases Epidemic IncX3 plasmids spreading carbapenemase genes in the United Arab Emirates and worldwide
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topic_title	RC109-216 Epidemic IncX3 plasmids spreading carbapenemase genes in the United Arab Emirates and worldwide Enterobacteriaceae carbapenemase genes IncX3 plasmid Middle-East
topic	misc RC109-216 misc Enterobacteriaceae misc carbapenemase genes misc IncX3 plasmid misc Middle-East misc Infectious and parasitic diseases
topic_unstemmed	misc RC109-216 misc Enterobacteriaceae misc carbapenemase genes misc IncX3 plasmid misc Middle-East misc Infectious and parasitic diseases
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title	Epidemic IncX3 plasmids spreading carbapenemase genes in the United Arab Emirates and worldwide
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title_full	Epidemic IncX3 plasmids spreading carbapenemase genes in the United Arab Emirates and worldwide
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author_browse	Mouftah SF Pál T Darwish D Ghazawi A Villa L Carattoli A Sonnevend Á
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title_sort	epidemic incx3 plasmids spreading carbapenemase genes in the united arab emirates and worldwide
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title_auth	Epidemic IncX3 plasmids spreading carbapenemase genes in the United Arab Emirates and worldwide
abstract	Shaimaa F Mouftah,1,* Tibor Pál,1,* Dania Darwish,1 Akela Ghazawi,1 Laura Villa,2 Alessandra Carattoli,3 Ágnes Sonnevend1,41Department of Medical Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates; 2Department of Infectious Diseases, Istituto Superiore di Sanitá, Rome, Italy; 3Department of Molecular Microbiology, University of Rome La Sapienza, Rome, Italy; 4Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Pécs, Hungary*These authors contributed equally to this work Purpose: Plasmids of the incompatibility group X type 3 (IncX3) were described carrying various carbapenemase genes in carbapenemase-producing Enterobacteriaceae (CPE) worldwide and in the United Arab Emirates (UAE), as well. To understand the driving force behind the emergence of such plasmids in the UAE, the relationship between IncX3 plasmids encountered locally and globally was investigated.Methods: CPE strains isolated in the UAE during 2009–2014 were screened by X3 PCR-based replicon typing. The clonal relationship of CPE carrying IncX3 plasmids was determined by multi-locus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). Complete sequence of selected IncX3 plasmids was determined. Phylogenetic relationship between the carbapenemase carrying IncX3 plasmids from the UAE and of those reported worldwide was established by comparing the plasmid backbones.Results: 10.2% of the 295 CPE tested were identified to carry IncX3 plasmids: 13 Escherichia coli, 13 Klebsiella pneumoniae, two Enterobacter cloacae, one Citrobacter freundii and one Morganella morganii isolate, respectively. Most of them were non-clonal; with small clusters of triplets and pairs of E. coli and K. pneumoniae, and a cluster of five K. pneumoniae ST11 exhibiting >90% similar PFGE patterns, respectively. The 30 isolates harbored either blaNDM-1, blaNDM-4, blaNDM-5, blaNDM-7, blaOXA-181 or blaKPC-2 carbapenemase genes on IncX3 plasmids. Phylogenetic analysis of the backbone region of IncX3 plasmids carrying various beta-lactamase genes from the UAE (n=23) and that of North-America, Europe, Asia and Australia (n=35) revealed three clusters based on the carbapenemase genes carried: plasmids harboring blaOXA-181 and blaNDM-5 formed two distinct groups, whereas backbones of plasmids with blaNDM-1, blaNDM-4 and blaNDM-7 clustered together. Each cluster contained plasmids of diverse geographical origin.Conclusion: The findings suggest that different carbapenemase gene carrying IncX3 plasmids encountered in the UAE do not evolve locally, rather are subtypes of this epidemic plasmid emerging in this country due to international transfer.Keywords: Enterobacterales, carbapenemase genes, IncX3 plasmid, Middle-East
abstractGer	Shaimaa F Mouftah,1,* Tibor Pál,1,* Dania Darwish,1 Akela Ghazawi,1 Laura Villa,2 Alessandra Carattoli,3 Ágnes Sonnevend1,41Department of Medical Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates; 2Department of Infectious Diseases, Istituto Superiore di Sanitá, Rome, Italy; 3Department of Molecular Microbiology, University of Rome La Sapienza, Rome, Italy; 4Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Pécs, Hungary*These authors contributed equally to this work Purpose: Plasmids of the incompatibility group X type 3 (IncX3) were described carrying various carbapenemase genes in carbapenemase-producing Enterobacteriaceae (CPE) worldwide and in the United Arab Emirates (UAE), as well. To understand the driving force behind the emergence of such plasmids in the UAE, the relationship between IncX3 plasmids encountered locally and globally was investigated.Methods: CPE strains isolated in the UAE during 2009–2014 were screened by X3 PCR-based replicon typing. The clonal relationship of CPE carrying IncX3 plasmids was determined by multi-locus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). Complete sequence of selected IncX3 plasmids was determined. Phylogenetic relationship between the carbapenemase carrying IncX3 plasmids from the UAE and of those reported worldwide was established by comparing the plasmid backbones.Results: 10.2% of the 295 CPE tested were identified to carry IncX3 plasmids: 13 Escherichia coli, 13 Klebsiella pneumoniae, two Enterobacter cloacae, one Citrobacter freundii and one Morganella morganii isolate, respectively. Most of them were non-clonal; with small clusters of triplets and pairs of E. coli and K. pneumoniae, and a cluster of five K. pneumoniae ST11 exhibiting >90% similar PFGE patterns, respectively. The 30 isolates harbored either blaNDM-1, blaNDM-4, blaNDM-5, blaNDM-7, blaOXA-181 or blaKPC-2 carbapenemase genes on IncX3 plasmids. Phylogenetic analysis of the backbone region of IncX3 plasmids carrying various beta-lactamase genes from the UAE (n=23) and that of North-America, Europe, Asia and Australia (n=35) revealed three clusters based on the carbapenemase genes carried: plasmids harboring blaOXA-181 and blaNDM-5 formed two distinct groups, whereas backbones of plasmids with blaNDM-1, blaNDM-4 and blaNDM-7 clustered together. Each cluster contained plasmids of diverse geographical origin.Conclusion: The findings suggest that different carbapenemase gene carrying IncX3 plasmids encountered in the UAE do not evolve locally, rather are subtypes of this epidemic plasmid emerging in this country due to international transfer.Keywords: Enterobacterales, carbapenemase genes, IncX3 plasmid, Middle-East
abstract_unstemmed	Shaimaa F Mouftah,1,* Tibor Pál,1,* Dania Darwish,1 Akela Ghazawi,1 Laura Villa,2 Alessandra Carattoli,3 Ágnes Sonnevend1,41Department of Medical Microbiology and Immunology, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates; 2Department of Infectious Diseases, Istituto Superiore di Sanitá, Rome, Italy; 3Department of Molecular Microbiology, University of Rome La Sapienza, Rome, Italy; 4Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Pécs, Hungary*These authors contributed equally to this work Purpose: Plasmids of the incompatibility group X type 3 (IncX3) were described carrying various carbapenemase genes in carbapenemase-producing Enterobacteriaceae (CPE) worldwide and in the United Arab Emirates (UAE), as well. To understand the driving force behind the emergence of such plasmids in the UAE, the relationship between IncX3 plasmids encountered locally and globally was investigated.Methods: CPE strains isolated in the UAE during 2009–2014 were screened by X3 PCR-based replicon typing. The clonal relationship of CPE carrying IncX3 plasmids was determined by multi-locus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE). Complete sequence of selected IncX3 plasmids was determined. Phylogenetic relationship between the carbapenemase carrying IncX3 plasmids from the UAE and of those reported worldwide was established by comparing the plasmid backbones.Results: 10.2% of the 295 CPE tested were identified to carry IncX3 plasmids: 13 Escherichia coli, 13 Klebsiella pneumoniae, two Enterobacter cloacae, one Citrobacter freundii and one Morganella morganii isolate, respectively. Most of them were non-clonal; with small clusters of triplets and pairs of E. coli and K. pneumoniae, and a cluster of five K. pneumoniae ST11 exhibiting >90% similar PFGE patterns, respectively. The 30 isolates harbored either blaNDM-1, blaNDM-4, blaNDM-5, blaNDM-7, blaOXA-181 or blaKPC-2 carbapenemase genes on IncX3 plasmids. Phylogenetic analysis of the backbone region of IncX3 plasmids carrying various beta-lactamase genes from the UAE (n=23) and that of North-America, Europe, Asia and Australia (n=35) revealed three clusters based on the carbapenemase genes carried: plasmids harboring blaOXA-181 and blaNDM-5 formed two distinct groups, whereas backbones of plasmids with blaNDM-1, blaNDM-4 and blaNDM-7 clustered together. Each cluster contained plasmids of diverse geographical origin.Conclusion: The findings suggest that different carbapenemase gene carrying IncX3 plasmids encountered in the UAE do not evolve locally, rather are subtypes of this epidemic plasmid emerging in this country due to international transfer.Keywords: Enterobacterales, carbapenemase genes, IncX3 plasmid, Middle-East
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title_short	Epidemic IncX3 plasmids spreading carbapenemase genes in the United Arab Emirates and worldwide
url	https://doaj.org/article/d7a353e2e8844833b83c96464455020f https://www.dovepress.com/epidemic-incx3-plasmids-spreading-carbapenemase-genes-in-the-united-ar-peer-reviewed-article-IDR https://doaj.org/toc/1178-6973
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Each cluster contained plasmids of diverse geographical origin.Conclusion: The findings suggest that different carbapenemase gene carrying IncX3 plasmids encountered in the UAE do not evolve locally, rather are subtypes of this epidemic plasmid emerging in this country due to international transfer.Keywords: Enterobacterales, carbapenemase genes, IncX3 plasmid, Middle-East</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Enterobacteriaceae</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">carbapenemase genes</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">IncX3 plasmid</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Middle-East</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Infectious and parasitic diseases</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Pál T</subfield><subfield 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and Drug Resistance</subfield><subfield code="d">Dove Medical Press, 2009</subfield><subfield code="g">(2019), Seite 1729-1742</subfield><subfield code="w">(DE-627)600305996</subfield><subfield code="w">(DE-600)2494856-1</subfield><subfield code="x">11786973</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">year:2019</subfield><subfield code="g">pages:1729-1742</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/d7a353e2e8844833b83c96464455020f</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://www.dovepress.com/epidemic-incx3-plasmids-spreading-carbapenemase-genes-in-the-united-ar-peer-reviewed-article-IDR</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/1178-6973</subfield><subfield code="y">Journal 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Epidemic IncX3 plasmids spreading carbapenemase genes in the United Arab Emirates and worldwide

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