Modulation of mitochondrial nucleoid structure during aging and by mtDNA content in Drosophila

Mitochondrial DNA (mtDNA) encodes gene products that are essential for oxidative phosphorylation. They organize as higher order nucleoid structures (mtNucleoids) that were shown to be critical for the maintenance of mtDNA stability and integrity. While mtNucleoid structures are associated with cellu...
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Autor*in:	Li-jie Wang [verfasserIn] Tian Hsu [verfasserIn] Hsiang-ling Lin [verfasserIn] Chi-yu Fu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	mitochondrial dna mitochondrial nucleoid transcription factor a (tfam) mitochondrial rna polymerase (mtrnapol) mitochondrial transcription factor b2 (mttfb2)

Übergeordnetes Werk:	In: Biology Open - The Company of Biologists, 2012, 10(2021), 6
Übergeordnetes Werk:	volume:10 ; year:2021 ; number:6

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1242/bio.058553

Katalog-ID:	DOAJ053007611

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520			\|a Mitochondrial DNA (mtDNA) encodes gene products that are essential for oxidative phosphorylation. They organize as higher order nucleoid structures (mtNucleoids) that were shown to be critical for the maintenance of mtDNA stability and integrity. While mtNucleoid structures are associated with cellular health, how they change in situ under physiological maturation and aging requires further investigation. In this study, we investigated the mtNucleoid assembly at an ultrastructural level in situ using the TFAM-Apex2 Drosophila model. We found that smaller and more compact TFAM-nucleoids are populated in the mitochondria of indirect flight muscle of aged flies. Furthermore, mtDNA transcription and replication were cross-regulated in the mtTFB2-knockdown flies as in the mtRNAPol-knockdown flies that resulted in reductions in mtDNA copy numbers and nucleoid-associated TFAM. Overall, our study reveals that the modulation of TFAM-nucleoid structure under physiological aging, which is critically regulated by mtDNA content.
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allfields	10.1242/bio.058553 doi (DE-627)DOAJ053007611 (DE-599)DOAJce45b3a6359a4c058ab561b34e8a3045 DE-627 ger DE-627 rakwb eng QH301-705.5 Li-jie Wang verfasserin aut Modulation of mitochondrial nucleoid structure during aging and by mtDNA content in Drosophila 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Mitochondrial DNA (mtDNA) encodes gene products that are essential for oxidative phosphorylation. They organize as higher order nucleoid structures (mtNucleoids) that were shown to be critical for the maintenance of mtDNA stability and integrity. While mtNucleoid structures are associated with cellular health, how they change in situ under physiological maturation and aging requires further investigation. In this study, we investigated the mtNucleoid assembly at an ultrastructural level in situ using the TFAM-Apex2 Drosophila model. We found that smaller and more compact TFAM-nucleoids are populated in the mitochondria of indirect flight muscle of aged flies. Furthermore, mtDNA transcription and replication were cross-regulated in the mtTFB2-knockdown flies as in the mtRNAPol-knockdown flies that resulted in reductions in mtDNA copy numbers and nucleoid-associated TFAM. Overall, our study reveals that the modulation of TFAM-nucleoid structure under physiological aging, which is critically regulated by mtDNA content. mitochondrial dna mitochondrial nucleoid transcription factor a (tfam) mitochondrial rna polymerase (mtrnapol) mitochondrial transcription factor b2 (mttfb2) Science Q Biology (General) Tian Hsu verfasserin aut Hsiang-ling Lin verfasserin aut Chi-yu Fu verfasserin aut In Biology Open The Company of Biologists, 2012 10(2021), 6 (DE-627)670214698 (DE-600)2632264-X 20466390 nnns volume:10 year:2021 number:6 https://doi.org/10.1242/bio.058553 kostenfrei https://doaj.org/article/ce45b3a6359a4c058ab561b34e8a3045 kostenfrei http://bio.biologists.org/content/10/6/bio058553 kostenfrei https://doaj.org/toc/2046-6390 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 6
spelling	10.1242/bio.058553 doi (DE-627)DOAJ053007611 (DE-599)DOAJce45b3a6359a4c058ab561b34e8a3045 DE-627 ger DE-627 rakwb eng QH301-705.5 Li-jie Wang verfasserin aut Modulation of mitochondrial nucleoid structure during aging and by mtDNA content in Drosophila 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Mitochondrial DNA (mtDNA) encodes gene products that are essential for oxidative phosphorylation. They organize as higher order nucleoid structures (mtNucleoids) that were shown to be critical for the maintenance of mtDNA stability and integrity. While mtNucleoid structures are associated with cellular health, how they change in situ under physiological maturation and aging requires further investigation. In this study, we investigated the mtNucleoid assembly at an ultrastructural level in situ using the TFAM-Apex2 Drosophila model. We found that smaller and more compact TFAM-nucleoids are populated in the mitochondria of indirect flight muscle of aged flies. Furthermore, mtDNA transcription and replication were cross-regulated in the mtTFB2-knockdown flies as in the mtRNAPol-knockdown flies that resulted in reductions in mtDNA copy numbers and nucleoid-associated TFAM. Overall, our study reveals that the modulation of TFAM-nucleoid structure under physiological aging, which is critically regulated by mtDNA content. mitochondrial dna mitochondrial nucleoid transcription factor a (tfam) mitochondrial rna polymerase (mtrnapol) mitochondrial transcription factor b2 (mttfb2) Science Q Biology (General) Tian Hsu verfasserin aut Hsiang-ling Lin verfasserin aut Chi-yu Fu verfasserin aut In Biology Open The Company of Biologists, 2012 10(2021), 6 (DE-627)670214698 (DE-600)2632264-X 20466390 nnns volume:10 year:2021 number:6 https://doi.org/10.1242/bio.058553 kostenfrei https://doaj.org/article/ce45b3a6359a4c058ab561b34e8a3045 kostenfrei http://bio.biologists.org/content/10/6/bio058553 kostenfrei https://doaj.org/toc/2046-6390 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 6
allfields_unstemmed	10.1242/bio.058553 doi (DE-627)DOAJ053007611 (DE-599)DOAJce45b3a6359a4c058ab561b34e8a3045 DE-627 ger DE-627 rakwb eng QH301-705.5 Li-jie Wang verfasserin aut Modulation of mitochondrial nucleoid structure during aging and by mtDNA content in Drosophila 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Mitochondrial DNA (mtDNA) encodes gene products that are essential for oxidative phosphorylation. They organize as higher order nucleoid structures (mtNucleoids) that were shown to be critical for the maintenance of mtDNA stability and integrity. While mtNucleoid structures are associated with cellular health, how they change in situ under physiological maturation and aging requires further investigation. In this study, we investigated the mtNucleoid assembly at an ultrastructural level in situ using the TFAM-Apex2 Drosophila model. We found that smaller and more compact TFAM-nucleoids are populated in the mitochondria of indirect flight muscle of aged flies. Furthermore, mtDNA transcription and replication were cross-regulated in the mtTFB2-knockdown flies as in the mtRNAPol-knockdown flies that resulted in reductions in mtDNA copy numbers and nucleoid-associated TFAM. Overall, our study reveals that the modulation of TFAM-nucleoid structure under physiological aging, which is critically regulated by mtDNA content. mitochondrial dna mitochondrial nucleoid transcription factor a (tfam) mitochondrial rna polymerase (mtrnapol) mitochondrial transcription factor b2 (mttfb2) Science Q Biology (General) Tian Hsu verfasserin aut Hsiang-ling Lin verfasserin aut Chi-yu Fu verfasserin aut In Biology Open The Company of Biologists, 2012 10(2021), 6 (DE-627)670214698 (DE-600)2632264-X 20466390 nnns volume:10 year:2021 number:6 https://doi.org/10.1242/bio.058553 kostenfrei https://doaj.org/article/ce45b3a6359a4c058ab561b34e8a3045 kostenfrei http://bio.biologists.org/content/10/6/bio058553 kostenfrei https://doaj.org/toc/2046-6390 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 6
allfieldsGer	10.1242/bio.058553 doi (DE-627)DOAJ053007611 (DE-599)DOAJce45b3a6359a4c058ab561b34e8a3045 DE-627 ger DE-627 rakwb eng QH301-705.5 Li-jie Wang verfasserin aut Modulation of mitochondrial nucleoid structure during aging and by mtDNA content in Drosophila 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Mitochondrial DNA (mtDNA) encodes gene products that are essential for oxidative phosphorylation. They organize as higher order nucleoid structures (mtNucleoids) that were shown to be critical for the maintenance of mtDNA stability and integrity. While mtNucleoid structures are associated with cellular health, how they change in situ under physiological maturation and aging requires further investigation. In this study, we investigated the mtNucleoid assembly at an ultrastructural level in situ using the TFAM-Apex2 Drosophila model. We found that smaller and more compact TFAM-nucleoids are populated in the mitochondria of indirect flight muscle of aged flies. Furthermore, mtDNA transcription and replication were cross-regulated in the mtTFB2-knockdown flies as in the mtRNAPol-knockdown flies that resulted in reductions in mtDNA copy numbers and nucleoid-associated TFAM. Overall, our study reveals that the modulation of TFAM-nucleoid structure under physiological aging, which is critically regulated by mtDNA content. mitochondrial dna mitochondrial nucleoid transcription factor a (tfam) mitochondrial rna polymerase (mtrnapol) mitochondrial transcription factor b2 (mttfb2) Science Q Biology (General) Tian Hsu verfasserin aut Hsiang-ling Lin verfasserin aut Chi-yu Fu verfasserin aut In Biology Open The Company of Biologists, 2012 10(2021), 6 (DE-627)670214698 (DE-600)2632264-X 20466390 nnns volume:10 year:2021 number:6 https://doi.org/10.1242/bio.058553 kostenfrei https://doaj.org/article/ce45b3a6359a4c058ab561b34e8a3045 kostenfrei http://bio.biologists.org/content/10/6/bio058553 kostenfrei https://doaj.org/toc/2046-6390 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 6
allfieldsSound	10.1242/bio.058553 doi (DE-627)DOAJ053007611 (DE-599)DOAJce45b3a6359a4c058ab561b34e8a3045 DE-627 ger DE-627 rakwb eng QH301-705.5 Li-jie Wang verfasserin aut Modulation of mitochondrial nucleoid structure during aging and by mtDNA content in Drosophila 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Mitochondrial DNA (mtDNA) encodes gene products that are essential for oxidative phosphorylation. They organize as higher order nucleoid structures (mtNucleoids) that were shown to be critical for the maintenance of mtDNA stability and integrity. While mtNucleoid structures are associated with cellular health, how they change in situ under physiological maturation and aging requires further investigation. In this study, we investigated the mtNucleoid assembly at an ultrastructural level in situ using the TFAM-Apex2 Drosophila model. We found that smaller and more compact TFAM-nucleoids are populated in the mitochondria of indirect flight muscle of aged flies. Furthermore, mtDNA transcription and replication were cross-regulated in the mtTFB2-knockdown flies as in the mtRNAPol-knockdown flies that resulted in reductions in mtDNA copy numbers and nucleoid-associated TFAM. Overall, our study reveals that the modulation of TFAM-nucleoid structure under physiological aging, which is critically regulated by mtDNA content. mitochondrial dna mitochondrial nucleoid transcription factor a (tfam) mitochondrial rna polymerase (mtrnapol) mitochondrial transcription factor b2 (mttfb2) Science Q Biology (General) Tian Hsu verfasserin aut Hsiang-ling Lin verfasserin aut Chi-yu Fu verfasserin aut In Biology Open The Company of Biologists, 2012 10(2021), 6 (DE-627)670214698 (DE-600)2632264-X 20466390 nnns volume:10 year:2021 number:6 https://doi.org/10.1242/bio.058553 kostenfrei https://doaj.org/article/ce45b3a6359a4c058ab561b34e8a3045 kostenfrei http://bio.biologists.org/content/10/6/bio058553 kostenfrei https://doaj.org/toc/2046-6390 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 10 2021 6
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callnumber-first	Q - Science
author	Li-jie Wang
spellingShingle	Li-jie Wang misc QH301-705.5 misc mitochondrial dna misc mitochondrial nucleoid misc transcription factor a (tfam) misc mitochondrial rna polymerase (mtrnapol) misc mitochondrial transcription factor b2 (mttfb2) misc Science misc Q misc Biology (General) Modulation of mitochondrial nucleoid structure during aging and by mtDNA content in Drosophila
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topic_title	QH301-705.5 Modulation of mitochondrial nucleoid structure during aging and by mtDNA content in Drosophila mitochondrial dna mitochondrial nucleoid transcription factor a (tfam) mitochondrial rna polymerase (mtrnapol) mitochondrial transcription factor b2 (mttfb2)
topic	misc QH301-705.5 misc mitochondrial dna misc mitochondrial nucleoid misc transcription factor a (tfam) misc mitochondrial rna polymerase (mtrnapol) misc mitochondrial transcription factor b2 (mttfb2) misc Science misc Q misc Biology (General)
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title	Modulation of mitochondrial nucleoid structure during aging and by mtDNA content in Drosophila
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title_full	Modulation of mitochondrial nucleoid structure during aging and by mtDNA content in Drosophila
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title_sort	modulation of mitochondrial nucleoid structure during aging and by mtdna content in drosophila
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title_auth	Modulation of mitochondrial nucleoid structure during aging and by mtDNA content in Drosophila
abstract	Mitochondrial DNA (mtDNA) encodes gene products that are essential for oxidative phosphorylation. They organize as higher order nucleoid structures (mtNucleoids) that were shown to be critical for the maintenance of mtDNA stability and integrity. While mtNucleoid structures are associated with cellular health, how they change in situ under physiological maturation and aging requires further investigation. In this study, we investigated the mtNucleoid assembly at an ultrastructural level in situ using the TFAM-Apex2 Drosophila model. We found that smaller and more compact TFAM-nucleoids are populated in the mitochondria of indirect flight muscle of aged flies. Furthermore, mtDNA transcription and replication were cross-regulated in the mtTFB2-knockdown flies as in the mtRNAPol-knockdown flies that resulted in reductions in mtDNA copy numbers and nucleoid-associated TFAM. Overall, our study reveals that the modulation of TFAM-nucleoid structure under physiological aging, which is critically regulated by mtDNA content.
abstractGer	Mitochondrial DNA (mtDNA) encodes gene products that are essential for oxidative phosphorylation. They organize as higher order nucleoid structures (mtNucleoids) that were shown to be critical for the maintenance of mtDNA stability and integrity. While mtNucleoid structures are associated with cellular health, how they change in situ under physiological maturation and aging requires further investigation. In this study, we investigated the mtNucleoid assembly at an ultrastructural level in situ using the TFAM-Apex2 Drosophila model. We found that smaller and more compact TFAM-nucleoids are populated in the mitochondria of indirect flight muscle of aged flies. Furthermore, mtDNA transcription and replication were cross-regulated in the mtTFB2-knockdown flies as in the mtRNAPol-knockdown flies that resulted in reductions in mtDNA copy numbers and nucleoid-associated TFAM. Overall, our study reveals that the modulation of TFAM-nucleoid structure under physiological aging, which is critically regulated by mtDNA content.
abstract_unstemmed	Mitochondrial DNA (mtDNA) encodes gene products that are essential for oxidative phosphorylation. They organize as higher order nucleoid structures (mtNucleoids) that were shown to be critical for the maintenance of mtDNA stability and integrity. While mtNucleoid structures are associated with cellular health, how they change in situ under physiological maturation and aging requires further investigation. In this study, we investigated the mtNucleoid assembly at an ultrastructural level in situ using the TFAM-Apex2 Drosophila model. We found that smaller and more compact TFAM-nucleoids are populated in the mitochondria of indirect flight muscle of aged flies. Furthermore, mtDNA transcription and replication were cross-regulated in the mtTFB2-knockdown flies as in the mtRNAPol-knockdown flies that resulted in reductions in mtDNA copy numbers and nucleoid-associated TFAM. Overall, our study reveals that the modulation of TFAM-nucleoid structure under physiological aging, which is critically regulated by mtDNA content.
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title_short	Modulation of mitochondrial nucleoid structure during aging and by mtDNA content in Drosophila
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Modulation of mitochondrial nucleoid structure during aging and by mtDNA content in Drosophila

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