Overexpression of an alfalfa glutathione S-transferase gene improved the saline-alkali tolerance of transgenic tobacco

Abiotic stresses restrict the productivity and quality of agricultural crops. Glutathione S-transferase (GST) utilizes glutathione to scavenge reactive oxygen species (ROS) that result from abiotic stresses. This study aimed to determine the expression pattern of the MsGSTU8 gene and its effects on...
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Autor*in:	Binghao Du [verfasserIn] Weidi Zhao [verfasserIn] Yimin An [verfasserIn] Yakun Li [verfasserIn] Xue Zhang [verfasserIn] Lili Song [verfasserIn] Changhong Guo [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	Alfalfa MsGSTU8 Saline-alkali tolerance ROS Antioxidant enzyme Transgenic tobacco

Übergeordnetes Werk:	In: Biology Open - The Company of Biologists, 2012, 8(2019), 9
Übergeordnetes Werk:	volume:8 ; year:2019 ; number:9

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1242/bio.043505

Katalog-ID:	DOAJ053055411

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allfieldsSound	10.1242/bio.043505 doi (DE-627)DOAJ053055411 (DE-599)DOAJ845abcdc4f6440369bd0b4641c7b9c5d DE-627 ger DE-627 rakwb eng QH301-705.5 Binghao Du verfasserin aut Overexpression of an alfalfa glutathione S-transferase gene improved the saline-alkali tolerance of transgenic tobacco 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Abiotic stresses restrict the productivity and quality of agricultural crops. Glutathione S-transferase (GST) utilizes glutathione to scavenge reactive oxygen species (ROS) that result from abiotic stresses. This study aimed to determine the expression pattern of the MsGSTU8 gene and its effects on saline-alkali tolerance. MsGSTU8, from alfalfa (Medicago sativa ‘Zhaodong’), was transformed into transgenic tobacco (Nicotiana tabacum) and overexpressed to determine its effects on saline-alkali tolerance. The gene products in alfalfa localized to the cytoplasm and the transcript levels were higher in the leaves than the roots and stems. Expression was strongly induced by cold, drought, salt and saline-alkali stresses as well as abscisic acid (ABA) treatments. The transgenic tobacco lines had significantly higher transcription levels of the abiotic stress-related genes and higher GST activity than the wild types. Transgenic tobacco lines with saline-alkali treatments maintained their chlorophyll content, showed improved antioxidant enzyme activity and soluble sugar levels, reduced ion leakage, O2.−, H2O2 accumulation and malondialdehyde content. Our results indicate that overexpression of MsGSTU8 could improve resistance to saline-alkali stresses by decreasing the accumulation of ROS and increasing the levels of antioxidant enzymes. Furthermore, they suggest that MsGSTU8 could be utilized for transgenic crop plant breeding. Alfalfa MsGSTU8 Saline-alkali tolerance ROS Antioxidant enzyme Transgenic tobacco Science Q Biology (General) Weidi Zhao verfasserin aut Yimin An verfasserin aut Yakun Li verfasserin aut Xue Zhang verfasserin aut Lili Song verfasserin aut Changhong Guo verfasserin aut In Biology Open The Company of Biologists, 2012 8(2019), 9 (DE-627)670214698 (DE-600)2632264-X 20466390 nnns volume:8 year:2019 number:9 https://doi.org/10.1242/bio.043505 kostenfrei https://doaj.org/article/845abcdc4f6440369bd0b4641c7b9c5d kostenfrei http://bio.biologists.org/content/8/9/bio043505 kostenfrei https://doaj.org/toc/2046-6390 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2019 9
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callnumber-first	Q - Science
author	Binghao Du
spellingShingle	Binghao Du misc QH301-705.5 misc Alfalfa misc MsGSTU8 misc Saline-alkali tolerance misc ROS misc Antioxidant enzyme misc Transgenic tobacco misc Science misc Q misc Biology (General) Overexpression of an alfalfa glutathione S-transferase gene improved the saline-alkali tolerance of transgenic tobacco
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topic_title	QH301-705.5 Overexpression of an alfalfa glutathione S-transferase gene improved the saline-alkali tolerance of transgenic tobacco Alfalfa MsGSTU8 Saline-alkali tolerance ROS Antioxidant enzyme Transgenic tobacco
topic	misc QH301-705.5 misc Alfalfa misc MsGSTU8 misc Saline-alkali tolerance misc ROS misc Antioxidant enzyme misc Transgenic tobacco misc Science misc Q misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc Alfalfa misc MsGSTU8 misc Saline-alkali tolerance misc ROS misc Antioxidant enzyme misc Transgenic tobacco misc Science misc Q misc Biology (General)
topic_browse	misc QH301-705.5 misc Alfalfa misc MsGSTU8 misc Saline-alkali tolerance misc ROS misc Antioxidant enzyme misc Transgenic tobacco misc Science misc Q misc Biology (General)
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title	Overexpression of an alfalfa glutathione S-transferase gene improved the saline-alkali tolerance of transgenic tobacco
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title_full	Overexpression of an alfalfa glutathione S-transferase gene improved the saline-alkali tolerance of transgenic tobacco
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author_browse	Binghao Du Weidi Zhao Yimin An Yakun Li Xue Zhang Lili Song Changhong Guo
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title_sort	overexpression of an alfalfa glutathione s-transferase gene improved the saline-alkali tolerance of transgenic tobacco
callnumber	QH301-705.5
title_auth	Overexpression of an alfalfa glutathione S-transferase gene improved the saline-alkali tolerance of transgenic tobacco
abstract	Abiotic stresses restrict the productivity and quality of agricultural crops. Glutathione S-transferase (GST) utilizes glutathione to scavenge reactive oxygen species (ROS) that result from abiotic stresses. This study aimed to determine the expression pattern of the MsGSTU8 gene and its effects on saline-alkali tolerance. MsGSTU8, from alfalfa (Medicago sativa ‘Zhaodong’), was transformed into transgenic tobacco (Nicotiana tabacum) and overexpressed to determine its effects on saline-alkali tolerance. The gene products in alfalfa localized to the cytoplasm and the transcript levels were higher in the leaves than the roots and stems. Expression was strongly induced by cold, drought, salt and saline-alkali stresses as well as abscisic acid (ABA) treatments. The transgenic tobacco lines had significantly higher transcription levels of the abiotic stress-related genes and higher GST activity than the wild types. Transgenic tobacco lines with saline-alkali treatments maintained their chlorophyll content, showed improved antioxidant enzyme activity and soluble sugar levels, reduced ion leakage, O2.−, H2O2 accumulation and malondialdehyde content. Our results indicate that overexpression of MsGSTU8 could improve resistance to saline-alkali stresses by decreasing the accumulation of ROS and increasing the levels of antioxidant enzymes. Furthermore, they suggest that MsGSTU8 could be utilized for transgenic crop plant breeding.
abstractGer	Abiotic stresses restrict the productivity and quality of agricultural crops. Glutathione S-transferase (GST) utilizes glutathione to scavenge reactive oxygen species (ROS) that result from abiotic stresses. This study aimed to determine the expression pattern of the MsGSTU8 gene and its effects on saline-alkali tolerance. MsGSTU8, from alfalfa (Medicago sativa ‘Zhaodong’), was transformed into transgenic tobacco (Nicotiana tabacum) and overexpressed to determine its effects on saline-alkali tolerance. The gene products in alfalfa localized to the cytoplasm and the transcript levels were higher in the leaves than the roots and stems. Expression was strongly induced by cold, drought, salt and saline-alkali stresses as well as abscisic acid (ABA) treatments. The transgenic tobacco lines had significantly higher transcription levels of the abiotic stress-related genes and higher GST activity than the wild types. Transgenic tobacco lines with saline-alkali treatments maintained their chlorophyll content, showed improved antioxidant enzyme activity and soluble sugar levels, reduced ion leakage, O2.−, H2O2 accumulation and malondialdehyde content. Our results indicate that overexpression of MsGSTU8 could improve resistance to saline-alkali stresses by decreasing the accumulation of ROS and increasing the levels of antioxidant enzymes. Furthermore, they suggest that MsGSTU8 could be utilized for transgenic crop plant breeding.
abstract_unstemmed	Abiotic stresses restrict the productivity and quality of agricultural crops. Glutathione S-transferase (GST) utilizes glutathione to scavenge reactive oxygen species (ROS) that result from abiotic stresses. This study aimed to determine the expression pattern of the MsGSTU8 gene and its effects on saline-alkali tolerance. MsGSTU8, from alfalfa (Medicago sativa ‘Zhaodong’), was transformed into transgenic tobacco (Nicotiana tabacum) and overexpressed to determine its effects on saline-alkali tolerance. The gene products in alfalfa localized to the cytoplasm and the transcript levels were higher in the leaves than the roots and stems. Expression was strongly induced by cold, drought, salt and saline-alkali stresses as well as abscisic acid (ABA) treatments. The transgenic tobacco lines had significantly higher transcription levels of the abiotic stress-related genes and higher GST activity than the wild types. Transgenic tobacco lines with saline-alkali treatments maintained their chlorophyll content, showed improved antioxidant enzyme activity and soluble sugar levels, reduced ion leakage, O2.−, H2O2 accumulation and malondialdehyde content. Our results indicate that overexpression of MsGSTU8 could improve resistance to saline-alkali stresses by decreasing the accumulation of ROS and increasing the levels of antioxidant enzymes. Furthermore, they suggest that MsGSTU8 could be utilized for transgenic crop plant breeding.
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title_short	Overexpression of an alfalfa glutathione S-transferase gene improved the saline-alkali tolerance of transgenic tobacco
url	https://doi.org/10.1242/bio.043505 https://doaj.org/article/845abcdc4f6440369bd0b4641c7b9c5d http://bio.biologists.org/content/8/9/bio043505 https://doaj.org/toc/2046-6390
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author2	Weidi Zhao Yimin An Yakun Li Xue Zhang Lili Song Changhong Guo
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Overexpression of an alfalfa glutathione S-transferase gene improved the saline-alkali tolerance of transgenic tobacco

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