Unusually Fast <i<bis</i<-Histidyl Coordination in a Plant Hemoglobin

The recently identified nonsymbiotic hemoglobin gene <i<MtGlb1-2</i< of the legume <i<Medicago truncatula</i< possesses unique properties as it generates four alternative splice forms encoding proteins with one or two heme domains. Here we investigate the ligand binding kinet...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Stefania Abbruzzetti [verfasserIn] Alex J. Barker [verfasserIn] Irene Villar [verfasserIn] Carmen Pérez-Rontomé [verfasserIn] Stefano Bruno [verfasserIn] Giulio Cerullo [verfasserIn] Cristiano Viappiani [verfasserIn] Manuel Becana [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	ultrafast spectroscopy plant hemoglobins CO rebinding kinetics iron/heme hexacoordination

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 22(2021), 5, p 2740
Übergeordnetes Werk:	volume:22 ; year:2021 ; number:5, p 2740

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.3390/ijms22052740

Katalog-ID:	DOAJ05309560X

Internformat


LEADER	01000caa a22002652 4500
001	DOAJ05309560X
003	DE-627
005	20240412191533.0
007	cr uuu---uuuuu
008	230227s2021 xx \|\|\|\|\|o 00\| \|\|eng c
024	7		\|a 10.3390/ijms22052740 \|2 doi
035			\|a (DE-627)DOAJ05309560X
035			\|a (DE-599)DOAJb25e27847c9649539137ba676a087cf2
040			\|a DE-627 \|b ger \|c DE-627 \|e rakwb
041			\|a eng
050		0	\|a QH301-705.5
050		0	\|a QD1-999
100	0		\|a Stefania Abbruzzetti \|e verfasserin \|4 aut
245	1	0	\|a Unusually Fast <i<bis</i<-Histidyl Coordination in a Plant Hemoglobin
264		1	\|c 2021
336			\|a Text \|b txt \|2 rdacontent
337			\|a Computermedien \|b c \|2 rdamedia
338			\|a Online-Ressource \|b cr \|2 rdacarrier
520			\|a The recently identified nonsymbiotic hemoglobin gene <i<MtGlb1-2</i< of the legume <i<Medicago truncatula</i< possesses unique properties as it generates four alternative splice forms encoding proteins with one or two heme domains. Here we investigate the ligand binding kinetics of MtGlb1-2.1 and MtGlb1-2.4, bearing two hemes and one heme, respectively. Unexpectedly, the overall time-course of ligand rebinding was unusually fast. Thus, we complemented nanosecond laser flash photolysis kinetics with data collected with a hybrid femtosecond–nanosecond pump–probe setup. Most photodissociated ligands are rebound geminately within a few nanoseconds, which leads to rates of the bimolecular rebinding to pentacoordinate species in the 10<sup<8</sup< M<sup<−1</sup<s<sup<−1</sup< range. Binding of the distal histidine to the heme competes with CO rebinding with extremely high rates (<i<k<sub<h</sub<</i< ~ 10<sup<5</sup< s<sup<−1</sup<). Histidine dissociation from the heme occurs with comparable rates, thus resulting in moderate equilibrium binding constants (<i<K<sub<H</sub<</i< ~ 1). The rate constants for ligation and deligation of distal histidine to the heme are the highest reported for any plant or vertebrate globin. The combination of microscopic rates results in unusually high overall ligand binding rate constants, a fact that contributes to explaining at the mechanistic level the extremely high reactivity of these proteins toward the physiological ligands oxygen, nitric oxide and nitrite.
650		4	\|a <i<Medicago truncatula</i<
650		4	\|a ultrafast spectroscopy
650		4	\|a plant hemoglobins
650		4	\|a CO rebinding kinetics
650		4	\|a iron/heme hexacoordination
653		0	\|a Biology (General)
653		0	\|a Chemistry
700	0		\|a Alex J. Barker \|e verfasserin \|4 aut
700	0		\|a Irene Villar \|e verfasserin \|4 aut
700	0		\|a Carmen Pérez-Rontomé \|e verfasserin \|4 aut
700	0		\|a Stefano Bruno \|e verfasserin \|4 aut
700	0		\|a Giulio Cerullo \|e verfasserin \|4 aut
700	0		\|a Cristiano Viappiani \|e verfasserin \|4 aut
700	0		\|a Manuel Becana \|e verfasserin \|4 aut
773	0	8	\|i In \|t International Journal of Molecular Sciences \|d MDPI AG, 2003 \|g 22(2021), 5, p 2740 \|w (DE-627)316340715 \|w (DE-600)2019364-6 \|x 14220067 \|7 nnns
773	1	8	\|g volume:22 \|g year:2021 \|g number:5, p 2740
856	4	0	\|u https://doi.org/10.3390/ijms22052740 \|z kostenfrei
856	4	0	\|u https://doaj.org/article/b25e27847c9649539137ba676a087cf2 \|z kostenfrei
856	4	0	\|u https://www.mdpi.com/1422-0067/22/5/2740 \|z kostenfrei
856	4	2	\|u https://doaj.org/toc/1661-6596 \|y Journal toc \|z kostenfrei
856	4	2	\|u https://doaj.org/toc/1422-0067 \|y Journal toc \|z kostenfrei
912			\|a GBV_USEFLAG_A
912			\|a SYSFLAG_A
912			\|a GBV_DOAJ
912			\|a GBV_ILN_20
912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_31
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_95
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_151
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_206
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_602
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4700
951			\|a AR
952			\|d 22 \|j 2021 \|e 5, p 2740

Indexfelder

author_variant	s a sa a j b ajb i v iv c p r cpr s b sb g c gc c v cv m b mb
matchkey_str	article:14220067:2021----::nsalfsiiiitdloriainnp
hierarchy_sort_str	2021
callnumber-subject-code	QH
publishDate	2021
allfields	10.3390/ijms22052740 doi (DE-627)DOAJ05309560X (DE-599)DOAJb25e27847c9649539137ba676a087cf2 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Stefania Abbruzzetti verfasserin aut Unusually Fast <i<bis</i<-Histidyl Coordination in a Plant Hemoglobin 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The recently identified nonsymbiotic hemoglobin gene <i<MtGlb1-2</i< of the legume <i<Medicago truncatula</i< possesses unique properties as it generates four alternative splice forms encoding proteins with one or two heme domains. Here we investigate the ligand binding kinetics of MtGlb1-2.1 and MtGlb1-2.4, bearing two hemes and one heme, respectively. Unexpectedly, the overall time-course of ligand rebinding was unusually fast. Thus, we complemented nanosecond laser flash photolysis kinetics with data collected with a hybrid femtosecond–nanosecond pump–probe setup. Most photodissociated ligands are rebound geminately within a few nanoseconds, which leads to rates of the bimolecular rebinding to pentacoordinate species in the 10<sup<8</sup< M<sup<−1</sup<s<sup<−1</sup< range. Binding of the distal histidine to the heme competes with CO rebinding with extremely high rates (<i<k<sub<h</sub<</i< ~ 10<sup<5</sup< s<sup<−1</sup<). Histidine dissociation from the heme occurs with comparable rates, thus resulting in moderate equilibrium binding constants (<i<K<sub<H</sub<</i< ~ 1). The rate constants for ligation and deligation of distal histidine to the heme are the highest reported for any plant or vertebrate globin. The combination of microscopic rates results in unusually high overall ligand binding rate constants, a fact that contributes to explaining at the mechanistic level the extremely high reactivity of these proteins toward the physiological ligands oxygen, nitric oxide and nitrite. <i<Medicago truncatula</i< ultrafast spectroscopy plant hemoglobins CO rebinding kinetics iron/heme hexacoordination Biology (General) Chemistry Alex J. Barker verfasserin aut Irene Villar verfasserin aut Carmen Pérez-Rontomé verfasserin aut Stefano Bruno verfasserin aut Giulio Cerullo verfasserin aut Cristiano Viappiani verfasserin aut Manuel Becana verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 5, p 2740 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:5, p 2740 https://doi.org/10.3390/ijms22052740 kostenfrei https://doaj.org/article/b25e27847c9649539137ba676a087cf2 kostenfrei https://www.mdpi.com/1422-0067/22/5/2740 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 5, p 2740
spelling	10.3390/ijms22052740 doi (DE-627)DOAJ05309560X (DE-599)DOAJb25e27847c9649539137ba676a087cf2 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Stefania Abbruzzetti verfasserin aut Unusually Fast <i<bis</i<-Histidyl Coordination in a Plant Hemoglobin 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The recently identified nonsymbiotic hemoglobin gene <i<MtGlb1-2</i< of the legume <i<Medicago truncatula</i< possesses unique properties as it generates four alternative splice forms encoding proteins with one or two heme domains. Here we investigate the ligand binding kinetics of MtGlb1-2.1 and MtGlb1-2.4, bearing two hemes and one heme, respectively. Unexpectedly, the overall time-course of ligand rebinding was unusually fast. Thus, we complemented nanosecond laser flash photolysis kinetics with data collected with a hybrid femtosecond–nanosecond pump–probe setup. Most photodissociated ligands are rebound geminately within a few nanoseconds, which leads to rates of the bimolecular rebinding to pentacoordinate species in the 10<sup<8</sup< M<sup<−1</sup<s<sup<−1</sup< range. Binding of the distal histidine to the heme competes with CO rebinding with extremely high rates (<i<k<sub<h</sub<</i< ~ 10<sup<5</sup< s<sup<−1</sup<). Histidine dissociation from the heme occurs with comparable rates, thus resulting in moderate equilibrium binding constants (<i<K<sub<H</sub<</i< ~ 1). The rate constants for ligation and deligation of distal histidine to the heme are the highest reported for any plant or vertebrate globin. The combination of microscopic rates results in unusually high overall ligand binding rate constants, a fact that contributes to explaining at the mechanistic level the extremely high reactivity of these proteins toward the physiological ligands oxygen, nitric oxide and nitrite. <i<Medicago truncatula</i< ultrafast spectroscopy plant hemoglobins CO rebinding kinetics iron/heme hexacoordination Biology (General) Chemistry Alex J. Barker verfasserin aut Irene Villar verfasserin aut Carmen Pérez-Rontomé verfasserin aut Stefano Bruno verfasserin aut Giulio Cerullo verfasserin aut Cristiano Viappiani verfasserin aut Manuel Becana verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 5, p 2740 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:5, p 2740 https://doi.org/10.3390/ijms22052740 kostenfrei https://doaj.org/article/b25e27847c9649539137ba676a087cf2 kostenfrei https://www.mdpi.com/1422-0067/22/5/2740 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 5, p 2740
allfields_unstemmed	10.3390/ijms22052740 doi (DE-627)DOAJ05309560X (DE-599)DOAJb25e27847c9649539137ba676a087cf2 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Stefania Abbruzzetti verfasserin aut Unusually Fast <i<bis</i<-Histidyl Coordination in a Plant Hemoglobin 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The recently identified nonsymbiotic hemoglobin gene <i<MtGlb1-2</i< of the legume <i<Medicago truncatula</i< possesses unique properties as it generates four alternative splice forms encoding proteins with one or two heme domains. Here we investigate the ligand binding kinetics of MtGlb1-2.1 and MtGlb1-2.4, bearing two hemes and one heme, respectively. Unexpectedly, the overall time-course of ligand rebinding was unusually fast. Thus, we complemented nanosecond laser flash photolysis kinetics with data collected with a hybrid femtosecond–nanosecond pump–probe setup. Most photodissociated ligands are rebound geminately within a few nanoseconds, which leads to rates of the bimolecular rebinding to pentacoordinate species in the 10<sup<8</sup< M<sup<−1</sup<s<sup<−1</sup< range. Binding of the distal histidine to the heme competes with CO rebinding with extremely high rates (<i<k<sub<h</sub<</i< ~ 10<sup<5</sup< s<sup<−1</sup<). Histidine dissociation from the heme occurs with comparable rates, thus resulting in moderate equilibrium binding constants (<i<K<sub<H</sub<</i< ~ 1). The rate constants for ligation and deligation of distal histidine to the heme are the highest reported for any plant or vertebrate globin. The combination of microscopic rates results in unusually high overall ligand binding rate constants, a fact that contributes to explaining at the mechanistic level the extremely high reactivity of these proteins toward the physiological ligands oxygen, nitric oxide and nitrite. <i<Medicago truncatula</i< ultrafast spectroscopy plant hemoglobins CO rebinding kinetics iron/heme hexacoordination Biology (General) Chemistry Alex J. Barker verfasserin aut Irene Villar verfasserin aut Carmen Pérez-Rontomé verfasserin aut Stefano Bruno verfasserin aut Giulio Cerullo verfasserin aut Cristiano Viappiani verfasserin aut Manuel Becana verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 5, p 2740 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:5, p 2740 https://doi.org/10.3390/ijms22052740 kostenfrei https://doaj.org/article/b25e27847c9649539137ba676a087cf2 kostenfrei https://www.mdpi.com/1422-0067/22/5/2740 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 5, p 2740
allfieldsGer	10.3390/ijms22052740 doi (DE-627)DOAJ05309560X (DE-599)DOAJb25e27847c9649539137ba676a087cf2 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Stefania Abbruzzetti verfasserin aut Unusually Fast <i<bis</i<-Histidyl Coordination in a Plant Hemoglobin 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The recently identified nonsymbiotic hemoglobin gene <i<MtGlb1-2</i< of the legume <i<Medicago truncatula</i< possesses unique properties as it generates four alternative splice forms encoding proteins with one or two heme domains. Here we investigate the ligand binding kinetics of MtGlb1-2.1 and MtGlb1-2.4, bearing two hemes and one heme, respectively. Unexpectedly, the overall time-course of ligand rebinding was unusually fast. Thus, we complemented nanosecond laser flash photolysis kinetics with data collected with a hybrid femtosecond–nanosecond pump–probe setup. Most photodissociated ligands are rebound geminately within a few nanoseconds, which leads to rates of the bimolecular rebinding to pentacoordinate species in the 10<sup<8</sup< M<sup<−1</sup<s<sup<−1</sup< range. Binding of the distal histidine to the heme competes with CO rebinding with extremely high rates (<i<k<sub<h</sub<</i< ~ 10<sup<5</sup< s<sup<−1</sup<). Histidine dissociation from the heme occurs with comparable rates, thus resulting in moderate equilibrium binding constants (<i<K<sub<H</sub<</i< ~ 1). The rate constants for ligation and deligation of distal histidine to the heme are the highest reported for any plant or vertebrate globin. The combination of microscopic rates results in unusually high overall ligand binding rate constants, a fact that contributes to explaining at the mechanistic level the extremely high reactivity of these proteins toward the physiological ligands oxygen, nitric oxide and nitrite. <i<Medicago truncatula</i< ultrafast spectroscopy plant hemoglobins CO rebinding kinetics iron/heme hexacoordination Biology (General) Chemistry Alex J. Barker verfasserin aut Irene Villar verfasserin aut Carmen Pérez-Rontomé verfasserin aut Stefano Bruno verfasserin aut Giulio Cerullo verfasserin aut Cristiano Viappiani verfasserin aut Manuel Becana verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 5, p 2740 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:5, p 2740 https://doi.org/10.3390/ijms22052740 kostenfrei https://doaj.org/article/b25e27847c9649539137ba676a087cf2 kostenfrei https://www.mdpi.com/1422-0067/22/5/2740 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 5, p 2740
allfieldsSound	10.3390/ijms22052740 doi (DE-627)DOAJ05309560X (DE-599)DOAJb25e27847c9649539137ba676a087cf2 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Stefania Abbruzzetti verfasserin aut Unusually Fast <i<bis</i<-Histidyl Coordination in a Plant Hemoglobin 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The recently identified nonsymbiotic hemoglobin gene <i<MtGlb1-2</i< of the legume <i<Medicago truncatula</i< possesses unique properties as it generates four alternative splice forms encoding proteins with one or two heme domains. Here we investigate the ligand binding kinetics of MtGlb1-2.1 and MtGlb1-2.4, bearing two hemes and one heme, respectively. Unexpectedly, the overall time-course of ligand rebinding was unusually fast. Thus, we complemented nanosecond laser flash photolysis kinetics with data collected with a hybrid femtosecond–nanosecond pump–probe setup. Most photodissociated ligands are rebound geminately within a few nanoseconds, which leads to rates of the bimolecular rebinding to pentacoordinate species in the 10<sup<8</sup< M<sup<−1</sup<s<sup<−1</sup< range. Binding of the distal histidine to the heme competes with CO rebinding with extremely high rates (<i<k<sub<h</sub<</i< ~ 10<sup<5</sup< s<sup<−1</sup<). Histidine dissociation from the heme occurs with comparable rates, thus resulting in moderate equilibrium binding constants (<i<K<sub<H</sub<</i< ~ 1). The rate constants for ligation and deligation of distal histidine to the heme are the highest reported for any plant or vertebrate globin. The combination of microscopic rates results in unusually high overall ligand binding rate constants, a fact that contributes to explaining at the mechanistic level the extremely high reactivity of these proteins toward the physiological ligands oxygen, nitric oxide and nitrite. <i<Medicago truncatula</i< ultrafast spectroscopy plant hemoglobins CO rebinding kinetics iron/heme hexacoordination Biology (General) Chemistry Alex J. Barker verfasserin aut Irene Villar verfasserin aut Carmen Pérez-Rontomé verfasserin aut Stefano Bruno verfasserin aut Giulio Cerullo verfasserin aut Cristiano Viappiani verfasserin aut Manuel Becana verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 22(2021), 5, p 2740 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:22 year:2021 number:5, p 2740 https://doi.org/10.3390/ijms22052740 kostenfrei https://doaj.org/article/b25e27847c9649539137ba676a087cf2 kostenfrei https://www.mdpi.com/1422-0067/22/5/2740 kostenfrei https://doaj.org/toc/1661-6596 Journal toc kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 22 2021 5, p 2740
language	English
source	In International Journal of Molecular Sciences 22(2021), 5, p 2740 volume:22 year:2021 number:5, p 2740
sourceStr	In International Journal of Molecular Sciences 22(2021), 5, p 2740 volume:22 year:2021 number:5, p 2740
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	<i<Medicago truncatula</i< ultrafast spectroscopy plant hemoglobins CO rebinding kinetics iron/heme hexacoordination Biology (General) Chemistry
isfreeaccess_bool	true
container_title	International Journal of Molecular Sciences
authorswithroles_txt_mv	Stefania Abbruzzetti @@aut@@ Alex J. Barker @@aut@@ Irene Villar @@aut@@ Carmen Pérez-Rontomé @@aut@@ Stefano Bruno @@aut@@ Giulio Cerullo @@aut@@ Cristiano Viappiani @@aut@@ Manuel Becana @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	316340715
id	DOAJ05309560X
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ05309560X</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20240412191533.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230227s2021 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.3390/ijms22052740</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ05309560X</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJb25e27847c9649539137ba676a087cf2</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QH301-705.5</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QD1-999</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Stefania Abbruzzetti</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Unusually Fast <i<bis</i<-Histidyl Coordination in a Plant Hemoglobin</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2021</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">The recently identified nonsymbiotic hemoglobin gene <i<MtGlb1-2</i< of the legume <i<Medicago truncatula</i< possesses unique properties as it generates four alternative splice forms encoding proteins with one or two heme domains. Here we investigate the ligand binding kinetics of MtGlb1-2.1 and MtGlb1-2.4, bearing two hemes and one heme, respectively. Unexpectedly, the overall time-course of ligand rebinding was unusually fast. Thus, we complemented nanosecond laser flash photolysis kinetics with data collected with a hybrid femtosecond–nanosecond pump–probe setup. Most photodissociated ligands are rebound geminately within a few nanoseconds, which leads to rates of the bimolecular rebinding to pentacoordinate species in the 10<sup<8</sup< M<sup<−1</sup<s<sup<−1</sup< range. Binding of the distal histidine to the heme competes with CO rebinding with extremely high rates (<i<k<sub<h</sub<</i< ~ 10<sup<5</sup< s<sup<−1</sup<). Histidine dissociation from the heme occurs with comparable rates, thus resulting in moderate equilibrium binding constants (<i<K<sub<H</sub<</i< ~ 1). The rate constants for ligation and deligation of distal histidine to the heme are the highest reported for any plant or vertebrate globin. The combination of microscopic rates results in unusually high overall ligand binding rate constants, a fact that contributes to explaining at the mechanistic level the extremely high reactivity of these proteins toward the physiological ligands oxygen, nitric oxide and nitrite.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a"><i<Medicago truncatula</i<</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">ultrafast spectroscopy</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">plant hemoglobins</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">CO rebinding kinetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">iron/heme hexacoordination</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Biology (General)</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Chemistry</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Alex J. Barker</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Irene Villar</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Carmen Pérez-Rontomé</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Stefano Bruno</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Giulio Cerullo</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Cristiano Viappiani</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Manuel Becana</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">International Journal of Molecular Sciences</subfield><subfield code="d">MDPI AG, 2003</subfield><subfield code="g">22(2021), 5, p 2740</subfield><subfield code="w">(DE-627)316340715</subfield><subfield code="w">(DE-600)2019364-6</subfield><subfield code="x">14220067</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:22</subfield><subfield code="g">year:2021</subfield><subfield code="g">number:5, p 2740</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.3390/ijms22052740</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/b25e27847c9649539137ba676a087cf2</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://www.mdpi.com/1422-0067/22/5/2740</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/1661-6596</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/1422-0067</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_206</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_224</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2009</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2011</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2055</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2111</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">22</subfield><subfield code="j">2021</subfield><subfield code="e">5, p 2740</subfield></datafield></record></collection>
callnumber-first	Q - Science
author	Stefania Abbruzzetti
spellingShingle	Stefania Abbruzzetti misc QH301-705.5 misc QD1-999 misc <i<Medicago truncatula</i< misc ultrafast spectroscopy misc plant hemoglobins misc CO rebinding kinetics misc iron/heme hexacoordination misc Biology (General) misc Chemistry Unusually Fast <i<bis</i<-Histidyl Coordination in a Plant Hemoglobin
authorStr	Stefania Abbruzzetti
ppnlink_with_tag_str_mv	@@773@@(DE-627)316340715
format	electronic Article
delete_txt_mv	keep
author_role	aut aut aut aut aut aut aut aut
collection	DOAJ
remote_str	true
callnumber-label	QH301-705
illustrated	Not Illustrated
issn	14220067
topic_title	QH301-705.5 QD1-999 Unusually Fast <i<bis</i<-Histidyl Coordination in a Plant Hemoglobin <i<Medicago truncatula</i< ultrafast spectroscopy plant hemoglobins CO rebinding kinetics iron/heme hexacoordination
topic	misc QH301-705.5 misc QD1-999 misc <i<Medicago truncatula</i< misc ultrafast spectroscopy misc plant hemoglobins misc CO rebinding kinetics misc iron/heme hexacoordination misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc <i<Medicago truncatula</i< misc ultrafast spectroscopy misc plant hemoglobins misc CO rebinding kinetics misc iron/heme hexacoordination misc Biology (General) misc Chemistry
topic_browse	misc QH301-705.5 misc QD1-999 misc <i<Medicago truncatula</i< misc ultrafast spectroscopy misc plant hemoglobins misc CO rebinding kinetics misc iron/heme hexacoordination misc Biology (General) misc Chemistry
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
format_main_str_mv	Text Zeitschrift/Artikel
carriertype_str_mv	cr
hierarchy_parent_title	International Journal of Molecular Sciences
hierarchy_parent_id	316340715
hierarchy_top_title	International Journal of Molecular Sciences
isfreeaccess_txt	true
familylinks_str_mv	(DE-627)316340715 (DE-600)2019364-6
title	Unusually Fast <i<bis</i<-Histidyl Coordination in a Plant Hemoglobin
ctrlnum	(DE-627)DOAJ05309560X (DE-599)DOAJb25e27847c9649539137ba676a087cf2
title_full	Unusually Fast <i<bis</i<-Histidyl Coordination in a Plant Hemoglobin
author_sort	Stefania Abbruzzetti
journal	International Journal of Molecular Sciences
journalStr	International Journal of Molecular Sciences
callnumber-first-code	Q
lang_code	eng
isOA_bool	true
recordtype	marc
publishDateSort	2021
contenttype_str_mv	txt
author_browse	Stefania Abbruzzetti Alex J. Barker Irene Villar Carmen Pérez-Rontomé Stefano Bruno Giulio Cerullo Cristiano Viappiani Manuel Becana
container_volume	22
class	QH301-705.5 QD1-999
format_se	Elektronische Aufsätze
author-letter	Stefania Abbruzzetti
doi_str_mv	10.3390/ijms22052740
author2-role	verfasserin
title_sort	unusually fast <i<bis</i<-histidyl coordination in a plant hemoglobin
callnumber	QH301-705.5
title_auth	Unusually Fast <i<bis</i<-Histidyl Coordination in a Plant Hemoglobin
abstract	The recently identified nonsymbiotic hemoglobin gene <i<MtGlb1-2</i< of the legume <i<Medicago truncatula</i< possesses unique properties as it generates four alternative splice forms encoding proteins with one or two heme domains. Here we investigate the ligand binding kinetics of MtGlb1-2.1 and MtGlb1-2.4, bearing two hemes and one heme, respectively. Unexpectedly, the overall time-course of ligand rebinding was unusually fast. Thus, we complemented nanosecond laser flash photolysis kinetics with data collected with a hybrid femtosecond–nanosecond pump–probe setup. Most photodissociated ligands are rebound geminately within a few nanoseconds, which leads to rates of the bimolecular rebinding to pentacoordinate species in the 10<sup<8</sup< M<sup<−1</sup<s<sup<−1</sup< range. Binding of the distal histidine to the heme competes with CO rebinding with extremely high rates (<i<k<sub<h</sub<</i< ~ 10<sup<5</sup< s<sup<−1</sup<). Histidine dissociation from the heme occurs with comparable rates, thus resulting in moderate equilibrium binding constants (<i<K<sub<H</sub<</i< ~ 1). The rate constants for ligation and deligation of distal histidine to the heme are the highest reported for any plant or vertebrate globin. The combination of microscopic rates results in unusually high overall ligand binding rate constants, a fact that contributes to explaining at the mechanistic level the extremely high reactivity of these proteins toward the physiological ligands oxygen, nitric oxide and nitrite.
abstractGer	The recently identified nonsymbiotic hemoglobin gene <i<MtGlb1-2</i< of the legume <i<Medicago truncatula</i< possesses unique properties as it generates four alternative splice forms encoding proteins with one or two heme domains. Here we investigate the ligand binding kinetics of MtGlb1-2.1 and MtGlb1-2.4, bearing two hemes and one heme, respectively. Unexpectedly, the overall time-course of ligand rebinding was unusually fast. Thus, we complemented nanosecond laser flash photolysis kinetics with data collected with a hybrid femtosecond–nanosecond pump–probe setup. Most photodissociated ligands are rebound geminately within a few nanoseconds, which leads to rates of the bimolecular rebinding to pentacoordinate species in the 10<sup<8</sup< M<sup<−1</sup<s<sup<−1</sup< range. Binding of the distal histidine to the heme competes with CO rebinding with extremely high rates (<i<k<sub<h</sub<</i< ~ 10<sup<5</sup< s<sup<−1</sup<). Histidine dissociation from the heme occurs with comparable rates, thus resulting in moderate equilibrium binding constants (<i<K<sub<H</sub<</i< ~ 1). The rate constants for ligation and deligation of distal histidine to the heme are the highest reported for any plant or vertebrate globin. The combination of microscopic rates results in unusually high overall ligand binding rate constants, a fact that contributes to explaining at the mechanistic level the extremely high reactivity of these proteins toward the physiological ligands oxygen, nitric oxide and nitrite.
abstract_unstemmed	The recently identified nonsymbiotic hemoglobin gene <i<MtGlb1-2</i< of the legume <i<Medicago truncatula</i< possesses unique properties as it generates four alternative splice forms encoding proteins with one or two heme domains. Here we investigate the ligand binding kinetics of MtGlb1-2.1 and MtGlb1-2.4, bearing two hemes and one heme, respectively. Unexpectedly, the overall time-course of ligand rebinding was unusually fast. Thus, we complemented nanosecond laser flash photolysis kinetics with data collected with a hybrid femtosecond–nanosecond pump–probe setup. Most photodissociated ligands are rebound geminately within a few nanoseconds, which leads to rates of the bimolecular rebinding to pentacoordinate species in the 10<sup<8</sup< M<sup<−1</sup<s<sup<−1</sup< range. Binding of the distal histidine to the heme competes with CO rebinding with extremely high rates (<i<k<sub<h</sub<</i< ~ 10<sup<5</sup< s<sup<−1</sup<). Histidine dissociation from the heme occurs with comparable rates, thus resulting in moderate equilibrium binding constants (<i<K<sub<H</sub<</i< ~ 1). The rate constants for ligation and deligation of distal histidine to the heme are the highest reported for any plant or vertebrate globin. The combination of microscopic rates results in unusually high overall ligand binding rate constants, a fact that contributes to explaining at the mechanistic level the extremely high reactivity of these proteins toward the physiological ligands oxygen, nitric oxide and nitrite.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
container_issue	5, p 2740
title_short	Unusually Fast <i<bis</i<-Histidyl Coordination in a Plant Hemoglobin
url	https://doi.org/10.3390/ijms22052740 https://doaj.org/article/b25e27847c9649539137ba676a087cf2 https://www.mdpi.com/1422-0067/22/5/2740 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067
remote_bool	true
author2	Alex J. Barker Irene Villar Carmen Pérez-Rontomé Stefano Bruno Giulio Cerullo Cristiano Viappiani Manuel Becana
author2Str	Alex J. Barker Irene Villar Carmen Pérez-Rontomé Stefano Bruno Giulio Cerullo Cristiano Viappiani Manuel Becana
ppnlink	316340715
callnumber-subject	QH - Natural History and Biology
mediatype_str_mv	c
isOA_txt	true
hochschulschrift_bool	false
doi_str	10.3390/ijms22052740
callnumber-a	QH301-705.5
up_date	2024-07-03T15:46:08.867Z
_version_	1803573336599429120
fullrecord_marcxml	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">DOAJ05309560X</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20240412191533.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230227s2021 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.3390/ijms22052740</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)DOAJ05309560X</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-599)DOAJb25e27847c9649539137ba676a087cf2</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QH301-705.5</subfield></datafield><datafield tag="050" ind1=" " ind2="0"><subfield code="a">QD1-999</subfield></datafield><datafield tag="100" ind1="0" ind2=" "><subfield code="a">Stefania Abbruzzetti</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Unusually Fast <i<bis</i<-Histidyl Coordination in a Plant Hemoglobin</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2021</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">The recently identified nonsymbiotic hemoglobin gene <i<MtGlb1-2</i< of the legume <i<Medicago truncatula</i< possesses unique properties as it generates four alternative splice forms encoding proteins with one or two heme domains. Here we investigate the ligand binding kinetics of MtGlb1-2.1 and MtGlb1-2.4, bearing two hemes and one heme, respectively. Unexpectedly, the overall time-course of ligand rebinding was unusually fast. Thus, we complemented nanosecond laser flash photolysis kinetics with data collected with a hybrid femtosecond–nanosecond pump–probe setup. Most photodissociated ligands are rebound geminately within a few nanoseconds, which leads to rates of the bimolecular rebinding to pentacoordinate species in the 10<sup<8</sup< M<sup<−1</sup<s<sup<−1</sup< range. Binding of the distal histidine to the heme competes with CO rebinding with extremely high rates (<i<k<sub<h</sub<</i< ~ 10<sup<5</sup< s<sup<−1</sup<). Histidine dissociation from the heme occurs with comparable rates, thus resulting in moderate equilibrium binding constants (<i<K<sub<H</sub<</i< ~ 1). The rate constants for ligation and deligation of distal histidine to the heme are the highest reported for any plant or vertebrate globin. The combination of microscopic rates results in unusually high overall ligand binding rate constants, a fact that contributes to explaining at the mechanistic level the extremely high reactivity of these proteins toward the physiological ligands oxygen, nitric oxide and nitrite.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a"><i<Medicago truncatula</i<</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">ultrafast spectroscopy</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">plant hemoglobins</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">CO rebinding kinetics</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">iron/heme hexacoordination</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Biology (General)</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Chemistry</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Alex J. Barker</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Irene Villar</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Carmen Pérez-Rontomé</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Stefano Bruno</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Giulio Cerullo</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Cristiano Viappiani</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="0" ind2=" "><subfield code="a">Manuel Becana</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">In</subfield><subfield code="t">International Journal of Molecular Sciences</subfield><subfield code="d">MDPI AG, 2003</subfield><subfield code="g">22(2021), 5, p 2740</subfield><subfield code="w">(DE-627)316340715</subfield><subfield code="w">(DE-600)2019364-6</subfield><subfield code="x">14220067</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:22</subfield><subfield code="g">year:2021</subfield><subfield code="g">number:5, p 2740</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.3390/ijms22052740</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doaj.org/article/b25e27847c9649539137ba676a087cf2</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://www.mdpi.com/1422-0067/22/5/2740</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/1661-6596</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="856" ind1="4" ind2="2"><subfield code="u">https://doaj.org/toc/1422-0067</subfield><subfield code="y">Journal toc</subfield><subfield code="z">kostenfrei</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_DOAJ</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_206</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_224</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2009</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2011</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2055</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2111</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4012</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4367</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">22</subfield><subfield code="j">2021</subfield><subfield code="e">5, p 2740</subfield></datafield></record></collection>
score	7.4013615

Nicht das Richtige dabei?

Schreiben Sie uns!

Unusually Fast <i<bis</i<-Histidyl Coordination in a Plant Hemoglobin

Nicht das Richtige dabei?

Zugang & Verfügbarkeit

Vorhandene Bände

Nicht das Richtige dabei?