Changes in microtubule-associated protein tau during peripheral nerve injury and regeneration

Tau, a primary component of microtubule-associated protein, promotes microtubule assembly and/or disassembly and maintains the stability of the microtubule structure. Although the importance of tau in neurodegenerative diseases has been well demonstrated, whether tau is involved in peripheral nerve...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Guang-bin Zha [verfasserIn] Mi Shen [verfasserIn] Xiao-song Gu [verfasserIn] Sheng Yi [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	nerve regeneration; sciatic nerve crush; microtubule-associated protein; tau; phosphorylated tau (Ser 404); tau hyper-phosphorylation; tau tubulin kinase 1; microtubule structure; microtubule assembly and disassembly; peripheral nervous system; neural regeneration

Übergeordnetes Werk:	In: Neural Regeneration Research - Wolters Kluwer Medknow Publications, 2014, 11(2016), 9, Seite 1506-1511
Übergeordnetes Werk:	volume:11 ; year:2016 ; number:9 ; pages:1506-1511

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.4103/1673-5374.191227

Katalog-ID:	DOAJ053115864

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source	In Neural Regeneration Research 11(2016), 9, Seite 1506-1511 volume:11 year:2016 number:9 pages:1506-1511
sourceStr	In Neural Regeneration Research 11(2016), 9, Seite 1506-1511 volume:11 year:2016 number:9 pages:1506-1511
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topic_facet	nerve regeneration; sciatic nerve crush; microtubule-associated protein; tau; phosphorylated tau (Ser 404); tau hyper-phosphorylation; tau tubulin kinase 1; microtubule structure; microtubule assembly and disassembly; peripheral nervous system; neural regeneration Neurology. Diseases of the nervous system
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callnumber-first	R - Medicine
author	Guang-bin Zha
spellingShingle	Guang-bin Zha misc RC346-429 misc nerve regeneration; sciatic nerve crush; microtubule-associated protein; tau; phosphorylated tau (Ser 404); tau hyper-phosphorylation; tau tubulin kinase 1; microtubule structure; microtubule assembly and disassembly; peripheral nervous system; neural regeneration misc Neurology. Diseases of the nervous system Changes in microtubule-associated protein tau during peripheral nerve injury and regeneration
authorStr	Guang-bin Zha
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topic_title	RC346-429 Changes in microtubule-associated protein tau during peripheral nerve injury and regeneration nerve regeneration; sciatic nerve crush; microtubule-associated protein; tau; phosphorylated tau (Ser 404); tau hyper-phosphorylation; tau tubulin kinase 1; microtubule structure; microtubule assembly and disassembly; peripheral nervous system; neural regeneration
topic	misc RC346-429 misc nerve regeneration; sciatic nerve crush; microtubule-associated protein; tau; phosphorylated tau (Ser 404); tau hyper-phosphorylation; tau tubulin kinase 1; microtubule structure; microtubule assembly and disassembly; peripheral nervous system; neural regeneration misc Neurology. Diseases of the nervous system
topic_unstemmed	misc RC346-429 misc nerve regeneration; sciatic nerve crush; microtubule-associated protein; tau; phosphorylated tau (Ser 404); tau hyper-phosphorylation; tau tubulin kinase 1; microtubule structure; microtubule assembly and disassembly; peripheral nervous system; neural regeneration misc Neurology. Diseases of the nervous system
topic_browse	misc RC346-429 misc nerve regeneration; sciatic nerve crush; microtubule-associated protein; tau; phosphorylated tau (Ser 404); tau hyper-phosphorylation; tau tubulin kinase 1; microtubule structure; microtubule assembly and disassembly; peripheral nervous system; neural regeneration misc Neurology. Diseases of the nervous system
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title	Changes in microtubule-associated protein tau during peripheral nerve injury and regeneration
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title_full	Changes in microtubule-associated protein tau during peripheral nerve injury and regeneration
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title_sort	changes in microtubule-associated protein tau during peripheral nerve injury and regeneration
callnumber	RC346-429
title_auth	Changes in microtubule-associated protein tau during peripheral nerve injury and regeneration
abstract	Tau, a primary component of microtubule-associated protein, promotes microtubule assembly and/or disassembly and maintains the stability of the microtubule structure. Although the importance of tau in neurodegenerative diseases has been well demonstrated, whether tau is involved in peripheral nerve regeneration remains unknown. In the current study, we obtained sciatic nerve tissue from adult rats 0, 1, 4, 7, and 14 days after sciatic nerve crush and examined tau mRNA and protein expression levels and the location of tau in the sciatic nerve following peripheral nerve injury. The results from our quantitative reverse transcription polymerase chain reaction analysis showed that compared with the uninjured control sciatic nerve, mRNA expression levels for both tau and tau tubulin kinase 1, a serine/threonine kinase that regulates tau phosphorylation, were decreased following peripheral nerve injury. Our western blot assay results suggested that the protein expression levels of tau and phosphorylated tau initially decreased 1 day post nerve injury but then gradually increased. The results of our immunohistochemical labeling showed that the location of tau protein was not altered by nerve injury. Thus, these results showed that the expression of tau was changed following sciatic nerve crush, suggesting that tau may be involved in peripheral nerve repair and regeneration.
abstractGer	Tau, a primary component of microtubule-associated protein, promotes microtubule assembly and/or disassembly and maintains the stability of the microtubule structure. Although the importance of tau in neurodegenerative diseases has been well demonstrated, whether tau is involved in peripheral nerve regeneration remains unknown. In the current study, we obtained sciatic nerve tissue from adult rats 0, 1, 4, 7, and 14 days after sciatic nerve crush and examined tau mRNA and protein expression levels and the location of tau in the sciatic nerve following peripheral nerve injury. The results from our quantitative reverse transcription polymerase chain reaction analysis showed that compared with the uninjured control sciatic nerve, mRNA expression levels for both tau and tau tubulin kinase 1, a serine/threonine kinase that regulates tau phosphorylation, were decreased following peripheral nerve injury. Our western blot assay results suggested that the protein expression levels of tau and phosphorylated tau initially decreased 1 day post nerve injury but then gradually increased. The results of our immunohistochemical labeling showed that the location of tau protein was not altered by nerve injury. Thus, these results showed that the expression of tau was changed following sciatic nerve crush, suggesting that tau may be involved in peripheral nerve repair and regeneration.
abstract_unstemmed	Tau, a primary component of microtubule-associated protein, promotes microtubule assembly and/or disassembly and maintains the stability of the microtubule structure. Although the importance of tau in neurodegenerative diseases has been well demonstrated, whether tau is involved in peripheral nerve regeneration remains unknown. In the current study, we obtained sciatic nerve tissue from adult rats 0, 1, 4, 7, and 14 days after sciatic nerve crush and examined tau mRNA and protein expression levels and the location of tau in the sciatic nerve following peripheral nerve injury. The results from our quantitative reverse transcription polymerase chain reaction analysis showed that compared with the uninjured control sciatic nerve, mRNA expression levels for both tau and tau tubulin kinase 1, a serine/threonine kinase that regulates tau phosphorylation, were decreased following peripheral nerve injury. Our western blot assay results suggested that the protein expression levels of tau and phosphorylated tau initially decreased 1 day post nerve injury but then gradually increased. The results of our immunohistochemical labeling showed that the location of tau protein was not altered by nerve injury. Thus, these results showed that the expression of tau was changed following sciatic nerve crush, suggesting that tau may be involved in peripheral nerve repair and regeneration.
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title_short	Changes in microtubule-associated protein tau during peripheral nerve injury and regeneration
url	https://doi.org/10.4103/1673-5374.191227 https://doaj.org/article/9df0bda6838c42f6bd530be553c76b7f http://www.nrronline.org/article.asp?issn=1673-5374;year=2016;volume=11;issue=9;spage=1506;epage=1511;aulast=Zha https://doaj.org/toc/1673-5374
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up_date	2024-07-03T15:53:02.502Z
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Changes in microtubule-associated protein tau during peripheral nerve injury and regeneration

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