Transcriptome analysis of reproductive tissue and intrauterine developmental stages of the tsetse fly (<it<Glossina morsitans morsitans</it<)

<p<Abstract</p< <p<Background</p< <p<Tsetse flies, vectors of African trypanosomes, undergo viviparous reproduction (the deposition of live offspring). This reproductive strategy results in a large maternal investment and the deposition of a small number of progeny during a female's lifespan. The reproductive biology of tsetse has been studied on a physiological level; however the molecular analysis of tsetse reproduction requires deeper investigation. To build a foundation from which to base molecular studies of tsetse reproduction, a cDNA library was generated from female tsetse (<it<Glossina morsitans morsitans</it<) reproductive tissues and the intrauterine developmental stages. 3438 expressed sequence tags were sequenced and analyzed.</p< <p<Results</p< <p<Analysis of a nonredundant catalogue of 1391 contigs resulted in 520 predicted proteins. 475 of these proteins were full length. We predict that 412 of these represent cytoplasmic proteins while 57 are secreted. Comparison of these proteins with other tissue specific tsetse cDNA libraries (salivary gland, fat body/milk gland, and midgut) identified 51 that are unique to the reproductive/immature cDNA library. 11 unique proteins were homologus to uncharacterized putative proteins within the NR database suggesting the identification of novel genes associated with reproductive functions in other insects (hypothetical conserved). The analysis also yielded seven putative proteins without significant homology to sequences present in the public database (unknown genes). These proteins may represent unique functions associated with tsetse's viviparous reproductive cycle. RT-PCR analysis of hypothetical conserved and unknown contigs was performed to determine basic tissue and stage specificity of the expression of these genes.</p< <p<Conclusion</p< <p<This paper identifies 51 putative proteins specific to a tsetse reproductive/immature EST library. 11 of these proteins correspond to hypothetical conserved genes and 7 proteins are tsetse specific.</p< Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Wu Yineng [verfasserIn] Berriman Matthew [verfasserIn] Ribeiro José MC [verfasserIn] Attardo Geoffrey M [verfasserIn] Aksoy Serap [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2010

Übergeordnetes Werk:	In: BMC Genomics - BMC, 2003, 11(2010), 1, p 160
Übergeordnetes Werk:	volume:11 ; year:2010 ; number:1, p 160

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1186/1471-2164-11-160

Katalog-ID:	DOAJ053228553

Internformat


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912			\|a GBV_ILN_170
912			\|a GBV_ILN_206
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912			\|a GBV_ILN_230
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912			\|a GBV_ILN_293
912			\|a GBV_ILN_602
912			\|a GBV_ILN_702
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912			\|a GBV_ILN_2003
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912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2008
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
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912			\|a GBV_ILN_2061
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912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2190
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912			\|a GBV_ILN_4325
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author_variant	w y wy b m bm r j m rjm a g m agm a s as
matchkey_str	article:14712164:2010----::rncitmaayiorpoutvtsuadnrueieeeomnasaeoteste
hierarchy_sort_str	2010
callnumber-subject-code	TP
publishDate	2010
allfields	10.1186/1471-2164-11-160 doi (DE-627)DOAJ053228553 (DE-599)DOAJ00a78f5abe6c44cbb17f717e0f0db6d4 DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Wu Yineng verfasserin aut Transcriptome analysis of reproductive tissue and intrauterine developmental stages of the tsetse fly (<it<Glossina morsitans morsitans</it<) 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Tsetse flies, vectors of African trypanosomes, undergo viviparous reproduction (the deposition of live offspring). This reproductive strategy results in a large maternal investment and the deposition of a small number of progeny during a female's lifespan. The reproductive biology of tsetse has been studied on a physiological level; however the molecular analysis of tsetse reproduction requires deeper investigation. To build a foundation from which to base molecular studies of tsetse reproduction, a cDNA library was generated from female tsetse (<it<Glossina morsitans morsitans</it<) reproductive tissues and the intrauterine developmental stages. 3438 expressed sequence tags were sequenced and analyzed.</p< <p<Results</p< <p<Analysis of a nonredundant catalogue of 1391 contigs resulted in 520 predicted proteins. 475 of these proteins were full length. We predict that 412 of these represent cytoplasmic proteins while 57 are secreted. Comparison of these proteins with other tissue specific tsetse cDNA libraries (salivary gland, fat body/milk gland, and midgut) identified 51 that are unique to the reproductive/immature cDNA library. 11 unique proteins were homologus to uncharacterized putative proteins within the NR database suggesting the identification of novel genes associated with reproductive functions in other insects (hypothetical conserved). The analysis also yielded seven putative proteins without significant homology to sequences present in the public database (unknown genes). These proteins may represent unique functions associated with tsetse's viviparous reproductive cycle. RT-PCR analysis of hypothetical conserved and unknown contigs was performed to determine basic tissue and stage specificity of the expression of these genes.</p< <p<Conclusion</p< <p<This paper identifies 51 putative proteins specific to a tsetse reproductive/immature EST library. 11 of these proteins correspond to hypothetical conserved genes and 7 proteins are tsetse specific.</p< Biotechnology Genetics Berriman Matthew verfasserin aut Ribeiro José MC verfasserin aut Attardo Geoffrey M verfasserin aut Aksoy Serap verfasserin aut In BMC Genomics BMC, 2003 11(2010), 1, p 160 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:11 year:2010 number:1, p 160 https://doi.org/10.1186/1471-2164-11-160 kostenfrei https://doaj.org/article/00a78f5abe6c44cbb17f717e0f0db6d4 kostenfrei http://www.biomedcentral.com/1471-2164/11/160 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2010 1, p 160
spelling	10.1186/1471-2164-11-160 doi (DE-627)DOAJ053228553 (DE-599)DOAJ00a78f5abe6c44cbb17f717e0f0db6d4 DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Wu Yineng verfasserin aut Transcriptome analysis of reproductive tissue and intrauterine developmental stages of the tsetse fly (<it<Glossina morsitans morsitans</it<) 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Tsetse flies, vectors of African trypanosomes, undergo viviparous reproduction (the deposition of live offspring). This reproductive strategy results in a large maternal investment and the deposition of a small number of progeny during a female's lifespan. The reproductive biology of tsetse has been studied on a physiological level; however the molecular analysis of tsetse reproduction requires deeper investigation. To build a foundation from which to base molecular studies of tsetse reproduction, a cDNA library was generated from female tsetse (<it<Glossina morsitans morsitans</it<) reproductive tissues and the intrauterine developmental stages. 3438 expressed sequence tags were sequenced and analyzed.</p< <p<Results</p< <p<Analysis of a nonredundant catalogue of 1391 contigs resulted in 520 predicted proteins. 475 of these proteins were full length. We predict that 412 of these represent cytoplasmic proteins while 57 are secreted. Comparison of these proteins with other tissue specific tsetse cDNA libraries (salivary gland, fat body/milk gland, and midgut) identified 51 that are unique to the reproductive/immature cDNA library. 11 unique proteins were homologus to uncharacterized putative proteins within the NR database suggesting the identification of novel genes associated with reproductive functions in other insects (hypothetical conserved). The analysis also yielded seven putative proteins without significant homology to sequences present in the public database (unknown genes). These proteins may represent unique functions associated with tsetse's viviparous reproductive cycle. RT-PCR analysis of hypothetical conserved and unknown contigs was performed to determine basic tissue and stage specificity of the expression of these genes.</p< <p<Conclusion</p< <p<This paper identifies 51 putative proteins specific to a tsetse reproductive/immature EST library. 11 of these proteins correspond to hypothetical conserved genes and 7 proteins are tsetse specific.</p< Biotechnology Genetics Berriman Matthew verfasserin aut Ribeiro José MC verfasserin aut Attardo Geoffrey M verfasserin aut Aksoy Serap verfasserin aut In BMC Genomics BMC, 2003 11(2010), 1, p 160 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:11 year:2010 number:1, p 160 https://doi.org/10.1186/1471-2164-11-160 kostenfrei https://doaj.org/article/00a78f5abe6c44cbb17f717e0f0db6d4 kostenfrei http://www.biomedcentral.com/1471-2164/11/160 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2010 1, p 160
allfields_unstemmed	10.1186/1471-2164-11-160 doi (DE-627)DOAJ053228553 (DE-599)DOAJ00a78f5abe6c44cbb17f717e0f0db6d4 DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Wu Yineng verfasserin aut Transcriptome analysis of reproductive tissue and intrauterine developmental stages of the tsetse fly (<it<Glossina morsitans morsitans</it<) 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Tsetse flies, vectors of African trypanosomes, undergo viviparous reproduction (the deposition of live offspring). This reproductive strategy results in a large maternal investment and the deposition of a small number of progeny during a female's lifespan. The reproductive biology of tsetse has been studied on a physiological level; however the molecular analysis of tsetse reproduction requires deeper investigation. To build a foundation from which to base molecular studies of tsetse reproduction, a cDNA library was generated from female tsetse (<it<Glossina morsitans morsitans</it<) reproductive tissues and the intrauterine developmental stages. 3438 expressed sequence tags were sequenced and analyzed.</p< <p<Results</p< <p<Analysis of a nonredundant catalogue of 1391 contigs resulted in 520 predicted proteins. 475 of these proteins were full length. We predict that 412 of these represent cytoplasmic proteins while 57 are secreted. Comparison of these proteins with other tissue specific tsetse cDNA libraries (salivary gland, fat body/milk gland, and midgut) identified 51 that are unique to the reproductive/immature cDNA library. 11 unique proteins were homologus to uncharacterized putative proteins within the NR database suggesting the identification of novel genes associated with reproductive functions in other insects (hypothetical conserved). The analysis also yielded seven putative proteins without significant homology to sequences present in the public database (unknown genes). These proteins may represent unique functions associated with tsetse's viviparous reproductive cycle. RT-PCR analysis of hypothetical conserved and unknown contigs was performed to determine basic tissue and stage specificity of the expression of these genes.</p< <p<Conclusion</p< <p<This paper identifies 51 putative proteins specific to a tsetse reproductive/immature EST library. 11 of these proteins correspond to hypothetical conserved genes and 7 proteins are tsetse specific.</p< Biotechnology Genetics Berriman Matthew verfasserin aut Ribeiro José MC verfasserin aut Attardo Geoffrey M verfasserin aut Aksoy Serap verfasserin aut In BMC Genomics BMC, 2003 11(2010), 1, p 160 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:11 year:2010 number:1, p 160 https://doi.org/10.1186/1471-2164-11-160 kostenfrei https://doaj.org/article/00a78f5abe6c44cbb17f717e0f0db6d4 kostenfrei http://www.biomedcentral.com/1471-2164/11/160 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2010 1, p 160
allfieldsGer	10.1186/1471-2164-11-160 doi (DE-627)DOAJ053228553 (DE-599)DOAJ00a78f5abe6c44cbb17f717e0f0db6d4 DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Wu Yineng verfasserin aut Transcriptome analysis of reproductive tissue and intrauterine developmental stages of the tsetse fly (<it<Glossina morsitans morsitans</it<) 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Tsetse flies, vectors of African trypanosomes, undergo viviparous reproduction (the deposition of live offspring). This reproductive strategy results in a large maternal investment and the deposition of a small number of progeny during a female's lifespan. The reproductive biology of tsetse has been studied on a physiological level; however the molecular analysis of tsetse reproduction requires deeper investigation. To build a foundation from which to base molecular studies of tsetse reproduction, a cDNA library was generated from female tsetse (<it<Glossina morsitans morsitans</it<) reproductive tissues and the intrauterine developmental stages. 3438 expressed sequence tags were sequenced and analyzed.</p< <p<Results</p< <p<Analysis of a nonredundant catalogue of 1391 contigs resulted in 520 predicted proteins. 475 of these proteins were full length. We predict that 412 of these represent cytoplasmic proteins while 57 are secreted. Comparison of these proteins with other tissue specific tsetse cDNA libraries (salivary gland, fat body/milk gland, and midgut) identified 51 that are unique to the reproductive/immature cDNA library. 11 unique proteins were homologus to uncharacterized putative proteins within the NR database suggesting the identification of novel genes associated with reproductive functions in other insects (hypothetical conserved). The analysis also yielded seven putative proteins without significant homology to sequences present in the public database (unknown genes). These proteins may represent unique functions associated with tsetse's viviparous reproductive cycle. RT-PCR analysis of hypothetical conserved and unknown contigs was performed to determine basic tissue and stage specificity of the expression of these genes.</p< <p<Conclusion</p< <p<This paper identifies 51 putative proteins specific to a tsetse reproductive/immature EST library. 11 of these proteins correspond to hypothetical conserved genes and 7 proteins are tsetse specific.</p< Biotechnology Genetics Berriman Matthew verfasserin aut Ribeiro José MC verfasserin aut Attardo Geoffrey M verfasserin aut Aksoy Serap verfasserin aut In BMC Genomics BMC, 2003 11(2010), 1, p 160 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:11 year:2010 number:1, p 160 https://doi.org/10.1186/1471-2164-11-160 kostenfrei https://doaj.org/article/00a78f5abe6c44cbb17f717e0f0db6d4 kostenfrei http://www.biomedcentral.com/1471-2164/11/160 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2010 1, p 160
allfieldsSound	10.1186/1471-2164-11-160 doi (DE-627)DOAJ053228553 (DE-599)DOAJ00a78f5abe6c44cbb17f717e0f0db6d4 DE-627 ger DE-627 rakwb eng TP248.13-248.65 QH426-470 Wu Yineng verfasserin aut Transcriptome analysis of reproductive tissue and intrauterine developmental stages of the tsetse fly (<it<Glossina morsitans morsitans</it<) 2010 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <p<Abstract</p< <p<Background</p< <p<Tsetse flies, vectors of African trypanosomes, undergo viviparous reproduction (the deposition of live offspring). This reproductive strategy results in a large maternal investment and the deposition of a small number of progeny during a female's lifespan. The reproductive biology of tsetse has been studied on a physiological level; however the molecular analysis of tsetse reproduction requires deeper investigation. To build a foundation from which to base molecular studies of tsetse reproduction, a cDNA library was generated from female tsetse (<it<Glossina morsitans morsitans</it<) reproductive tissues and the intrauterine developmental stages. 3438 expressed sequence tags were sequenced and analyzed.</p< <p<Results</p< <p<Analysis of a nonredundant catalogue of 1391 contigs resulted in 520 predicted proteins. 475 of these proteins were full length. We predict that 412 of these represent cytoplasmic proteins while 57 are secreted. Comparison of these proteins with other tissue specific tsetse cDNA libraries (salivary gland, fat body/milk gland, and midgut) identified 51 that are unique to the reproductive/immature cDNA library. 11 unique proteins were homologus to uncharacterized putative proteins within the NR database suggesting the identification of novel genes associated with reproductive functions in other insects (hypothetical conserved). The analysis also yielded seven putative proteins without significant homology to sequences present in the public database (unknown genes). These proteins may represent unique functions associated with tsetse's viviparous reproductive cycle. RT-PCR analysis of hypothetical conserved and unknown contigs was performed to determine basic tissue and stage specificity of the expression of these genes.</p< <p<Conclusion</p< <p<This paper identifies 51 putative proteins specific to a tsetse reproductive/immature EST library. 11 of these proteins correspond to hypothetical conserved genes and 7 proteins are tsetse specific.</p< Biotechnology Genetics Berriman Matthew verfasserin aut Ribeiro José MC verfasserin aut Attardo Geoffrey M verfasserin aut Aksoy Serap verfasserin aut In BMC Genomics BMC, 2003 11(2010), 1, p 160 (DE-627)326644954 (DE-600)2041499-7 14712164 nnns volume:11 year:2010 number:1, p 160 https://doi.org/10.1186/1471-2164-11-160 kostenfrei https://doaj.org/article/00a78f5abe6c44cbb17f717e0f0db6d4 kostenfrei http://www.biomedcentral.com/1471-2164/11/160 kostenfrei https://doaj.org/toc/1471-2164 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2010 1, p 160
language	English
source	In BMC Genomics 11(2010), 1, p 160 volume:11 year:2010 number:1, p 160
sourceStr	In BMC Genomics 11(2010), 1, p 160 volume:11 year:2010 number:1, p 160
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Biotechnology Genetics
isfreeaccess_bool	true
container_title	BMC Genomics
authorswithroles_txt_mv	Wu Yineng @@aut@@ Berriman Matthew @@aut@@ Ribeiro José MC @@aut@@ Attardo Geoffrey M @@aut@@ Aksoy Serap @@aut@@
publishDateDaySort_date	2010-01-01T00:00:00Z
hierarchy_top_id	326644954
id	DOAJ053228553
language_de	englisch
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This reproductive strategy results in a large maternal investment and the deposition of a small number of progeny during a female's lifespan. The reproductive biology of tsetse has been studied on a physiological level; however the molecular analysis of tsetse reproduction requires deeper investigation. To build a foundation from which to base molecular studies of tsetse reproduction, a cDNA library was generated from female tsetse (<it<Glossina morsitans morsitans</it<) reproductive tissues and the intrauterine developmental stages. 3438 expressed sequence tags were sequenced and analyzed.</p< <p<Results</p< <p<Analysis of a nonredundant catalogue of 1391 contigs resulted in 520 predicted proteins. 475 of these proteins were full length. We predict that 412 of these represent cytoplasmic proteins while 57 are secreted. Comparison of these proteins with other tissue specific tsetse cDNA libraries (salivary gland, fat body/milk gland, and midgut) identified 51 that are unique to the reproductive/immature cDNA library. 11 unique proteins were homologus to uncharacterized putative proteins within the NR database suggesting the identification of novel genes associated with reproductive functions in other insects (hypothetical conserved). The analysis also yielded seven putative proteins without significant homology to sequences present in the public database (unknown genes). These proteins may represent unique functions associated with tsetse's viviparous reproductive cycle. 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callnumber-first	T - Technology
author	Wu Yineng
spellingShingle	Wu Yineng misc TP248.13-248.65 misc QH426-470 misc Biotechnology misc Genetics Transcriptome analysis of reproductive tissue and intrauterine developmental stages of the tsetse fly (<it<Glossina morsitans morsitans</it<)
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topic_title	TP248.13-248.65 QH426-470 Transcriptome analysis of reproductive tissue and intrauterine developmental stages of the tsetse fly (<it<Glossina morsitans morsitans</it<)
topic	misc TP248.13-248.65 misc QH426-470 misc Biotechnology misc Genetics
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title	Transcriptome analysis of reproductive tissue and intrauterine developmental stages of the tsetse fly (<it<Glossina morsitans morsitans</it<)
ctrlnum	(DE-627)DOAJ053228553 (DE-599)DOAJ00a78f5abe6c44cbb17f717e0f0db6d4
title_full	Transcriptome analysis of reproductive tissue and intrauterine developmental stages of the tsetse fly (<it<Glossina morsitans morsitans</it<)
author_sort	Wu Yineng
journal	BMC Genomics
journalStr	BMC Genomics
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author_browse	Wu Yineng Berriman Matthew Ribeiro José MC Attardo Geoffrey M Aksoy Serap
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class	TP248.13-248.65 QH426-470
format_se	Elektronische Aufsätze
author-letter	Wu Yineng
doi_str_mv	10.1186/1471-2164-11-160
author2-role	verfasserin
title_sort	transcriptome analysis of reproductive tissue and intrauterine developmental stages of the tsetse fly (<it<glossina morsitans morsitans</it<)
callnumber	TP248.13-248.65
title_auth	Transcriptome analysis of reproductive tissue and intrauterine developmental stages of the tsetse fly (<it<Glossina morsitans morsitans</it<)
abstract	<p<Abstract</p< <p<Background</p< <p<Tsetse flies, vectors of African trypanosomes, undergo viviparous reproduction (the deposition of live offspring). This reproductive strategy results in a large maternal investment and the deposition of a small number of progeny during a female's lifespan. The reproductive biology of tsetse has been studied on a physiological level; however the molecular analysis of tsetse reproduction requires deeper investigation. To build a foundation from which to base molecular studies of tsetse reproduction, a cDNA library was generated from female tsetse (<it<Glossina morsitans morsitans</it<) reproductive tissues and the intrauterine developmental stages. 3438 expressed sequence tags were sequenced and analyzed.</p< <p<Results</p< <p<Analysis of a nonredundant catalogue of 1391 contigs resulted in 520 predicted proteins. 475 of these proteins were full length. We predict that 412 of these represent cytoplasmic proteins while 57 are secreted. Comparison of these proteins with other tissue specific tsetse cDNA libraries (salivary gland, fat body/milk gland, and midgut) identified 51 that are unique to the reproductive/immature cDNA library. 11 unique proteins were homologus to uncharacterized putative proteins within the NR database suggesting the identification of novel genes associated with reproductive functions in other insects (hypothetical conserved). The analysis also yielded seven putative proteins without significant homology to sequences present in the public database (unknown genes). These proteins may represent unique functions associated with tsetse's viviparous reproductive cycle. RT-PCR analysis of hypothetical conserved and unknown contigs was performed to determine basic tissue and stage specificity of the expression of these genes.</p< <p<Conclusion</p< <p<This paper identifies 51 putative proteins specific to a tsetse reproductive/immature EST library. 11 of these proteins correspond to hypothetical conserved genes and 7 proteins are tsetse specific.</p<
abstractGer	<p<Abstract</p< <p<Background</p< <p<Tsetse flies, vectors of African trypanosomes, undergo viviparous reproduction (the deposition of live offspring). This reproductive strategy results in a large maternal investment and the deposition of a small number of progeny during a female's lifespan. The reproductive biology of tsetse has been studied on a physiological level; however the molecular analysis of tsetse reproduction requires deeper investigation. To build a foundation from which to base molecular studies of tsetse reproduction, a cDNA library was generated from female tsetse (<it<Glossina morsitans morsitans</it<) reproductive tissues and the intrauterine developmental stages. 3438 expressed sequence tags were sequenced and analyzed.</p< <p<Results</p< <p<Analysis of a nonredundant catalogue of 1391 contigs resulted in 520 predicted proteins. 475 of these proteins were full length. We predict that 412 of these represent cytoplasmic proteins while 57 are secreted. Comparison of these proteins with other tissue specific tsetse cDNA libraries (salivary gland, fat body/milk gland, and midgut) identified 51 that are unique to the reproductive/immature cDNA library. 11 unique proteins were homologus to uncharacterized putative proteins within the NR database suggesting the identification of novel genes associated with reproductive functions in other insects (hypothetical conserved). The analysis also yielded seven putative proteins without significant homology to sequences present in the public database (unknown genes). These proteins may represent unique functions associated with tsetse's viviparous reproductive cycle. RT-PCR analysis of hypothetical conserved and unknown contigs was performed to determine basic tissue and stage specificity of the expression of these genes.</p< <p<Conclusion</p< <p<This paper identifies 51 putative proteins specific to a tsetse reproductive/immature EST library. 11 of these proteins correspond to hypothetical conserved genes and 7 proteins are tsetse specific.</p<
abstract_unstemmed	<p<Abstract</p< <p<Background</p< <p<Tsetse flies, vectors of African trypanosomes, undergo viviparous reproduction (the deposition of live offspring). This reproductive strategy results in a large maternal investment and the deposition of a small number of progeny during a female's lifespan. The reproductive biology of tsetse has been studied on a physiological level; however the molecular analysis of tsetse reproduction requires deeper investigation. To build a foundation from which to base molecular studies of tsetse reproduction, a cDNA library was generated from female tsetse (<it<Glossina morsitans morsitans</it<) reproductive tissues and the intrauterine developmental stages. 3438 expressed sequence tags were sequenced and analyzed.</p< <p<Results</p< <p<Analysis of a nonredundant catalogue of 1391 contigs resulted in 520 predicted proteins. 475 of these proteins were full length. We predict that 412 of these represent cytoplasmic proteins while 57 are secreted. Comparison of these proteins with other tissue specific tsetse cDNA libraries (salivary gland, fat body/milk gland, and midgut) identified 51 that are unique to the reproductive/immature cDNA library. 11 unique proteins were homologus to uncharacterized putative proteins within the NR database suggesting the identification of novel genes associated with reproductive functions in other insects (hypothetical conserved). The analysis also yielded seven putative proteins without significant homology to sequences present in the public database (unknown genes). These proteins may represent unique functions associated with tsetse's viviparous reproductive cycle. RT-PCR analysis of hypothetical conserved and unknown contigs was performed to determine basic tissue and stage specificity of the expression of these genes.</p< <p<Conclusion</p< <p<This paper identifies 51 putative proteins specific to a tsetse reproductive/immature EST library. 11 of these proteins correspond to hypothetical conserved genes and 7 proteins are tsetse specific.</p<
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title_short	Transcriptome analysis of reproductive tissue and intrauterine developmental stages of the tsetse fly (<it<Glossina morsitans morsitans</it<)
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Transcriptome analysis of reproductive tissue and intrauterine developmental stages of the tsetse fly (<it<Glossina morsitans morsitans</it<)

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