Using AFLP-RGA markers to assess genetic diversity among pigeon pea (Cajanus cajan) genotypes in relation to major diseases

Resistance gene analog (RGA)-anchored amplified fragment length polymorphism (AFLP-RGA) marker system was used in order to evaluate genetic relationships among 22 pigeon pea genotypes with varied responses to Fusarium wilt and sterility mosaic disease. Five AFLP-RGA primer combinations (E-CAG/wlrk-S...
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Autor*in:	Prakash G Pati [verfasserIn] Subhojit Datta [verfasserIn] Ikechukwu O Agbagwa [verfasserIn] Indra Prakash Singh [verfasserIn] Khela Ram Soren [verfasserIn] Alok Das [verfasserIn] Arbind Kumar Choudhary [verfasserIn] Sushil Kumar Chaturvedi [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2014

Schlagwörter:	AFLP-RGA Cajanus cajan genetic diversity molecular markers polymorphism

Übergeordnetes Werk:	In: Acta Botânica Brasílica - Sociedade Botânica do Brasil, 2004, 28(2014), 2, Seite 198-205
Übergeordnetes Werk:	volume:28 ; year:2014 ; number:2 ; pages:198-205

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1590/S0102-33062014000200007

Katalog-ID:	DOAJ053310241

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520			\|a Resistance gene analog (RGA)-anchored amplified fragment length polymorphism (AFLP-RGA) marker system was used in order to evaluate genetic relationships among 22 pigeon pea genotypes with varied responses to Fusarium wilt and sterility mosaic disease. Five AFLP-RGA primer combinations (E-CAG/wlrk-S, M-GTG/wlrk-S, M-GTG/wlrk-AS, E-CAT/S1-INV and E-CAG/wlrk-AS) produced 173 scorable fragments, of which 157 (90.7%) were polymorphic, with an average of 31.4 fragments per primer combination. The polymorphism rates obtained with the five primers were 83.3%, 92.0%, 92.3%, 93.0% and 93.1%, respectively. Mean polymorphic information content (PIC) values ranged from 0.24 (with E-CAT/S1-INV) to 0.30 (with E-CAG/wlrk-AS), whereas resolving power (RP) values varied from 11.06 (with M-GTG/wlrk-S) to 25.51 (with E-CAG/wlrk-AS) and marker index (MI) values ranged from 5.98 (with M-GTG/wlrk-S) to 12.30 (with E-CAG/wlrk-AS). We identified a positive correlation between MI and RP (r²=0.98, p<0.05), stronger that that observed for the comparison between PIC and RP (r²=0.88, p<0.05). That implies that either MI or RP is the best parameter for selecting more informative AFLP-RGA primer combinations. The Jaccard coefficient ranged from 0.07 to 0.72, suggesting a broad genetic base in the genotypes studied. A neighbor-joining tree, based on the unweighted pair group method with arithmetic mean, distinguished cultivated species from wild species. The grouping of resistant genotypes in different clusters would help in the selection of suitable donors for resistance breeding in pigeon pea.
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allfields	10.1590/S0102-33062014000200007 doi (DE-627)DOAJ053310241 (DE-599)DOAJ2d2a0b7915f944469a8b49515d304c1d DE-627 ger DE-627 rakwb eng QK1-989 Prakash G Pati verfasserin aut Using AFLP-RGA markers to assess genetic diversity among pigeon pea (Cajanus cajan) genotypes in relation to major diseases 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Resistance gene analog (RGA)-anchored amplified fragment length polymorphism (AFLP-RGA) marker system was used in order to evaluate genetic relationships among 22 pigeon pea genotypes with varied responses to Fusarium wilt and sterility mosaic disease. Five AFLP-RGA primer combinations (E-CAG/wlrk-S, M-GTG/wlrk-S, M-GTG/wlrk-AS, E-CAT/S1-INV and E-CAG/wlrk-AS) produced 173 scorable fragments, of which 157 (90.7%) were polymorphic, with an average of 31.4 fragments per primer combination. The polymorphism rates obtained with the five primers were 83.3%, 92.0%, 92.3%, 93.0% and 93.1%, respectively. Mean polymorphic information content (PIC) values ranged from 0.24 (with E-CAT/S1-INV) to 0.30 (with E-CAG/wlrk-AS), whereas resolving power (RP) values varied from 11.06 (with M-GTG/wlrk-S) to 25.51 (with E-CAG/wlrk-AS) and marker index (MI) values ranged from 5.98 (with M-GTG/wlrk-S) to 12.30 (with E-CAG/wlrk-AS). We identified a positive correlation between MI and RP (r²=0.98, p<0.05), stronger that that observed for the comparison between PIC and RP (r²=0.88, p<0.05). That implies that either MI or RP is the best parameter for selecting more informative AFLP-RGA primer combinations. The Jaccard coefficient ranged from 0.07 to 0.72, suggesting a broad genetic base in the genotypes studied. A neighbor-joining tree, based on the unweighted pair group method with arithmetic mean, distinguished cultivated species from wild species. The grouping of resistant genotypes in different clusters would help in the selection of suitable donors for resistance breeding in pigeon pea. AFLP-RGA Cajanus cajan genetic diversity molecular markers polymorphism Botany Subhojit Datta verfasserin aut Ikechukwu O Agbagwa verfasserin aut Indra Prakash Singh verfasserin aut Khela Ram Soren verfasserin aut Alok Das verfasserin aut Arbind Kumar Choudhary verfasserin aut Sushil Kumar Chaturvedi verfasserin aut In Acta Botânica Brasílica Sociedade Botânica do Brasil, 2004 28(2014), 2, Seite 198-205 (DE-627)332926419 (DE-600)2055440-0 1677941X nnns volume:28 year:2014 number:2 pages:198-205 https://doi.org/10.1590/S0102-33062014000200007 kostenfrei https://doaj.org/article/2d2a0b7915f944469a8b49515d304c1d kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000200007&lng=en&tlng=en kostenfrei https://doaj.org/toc/1677-941X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2014 2 198-205
spelling	10.1590/S0102-33062014000200007 doi (DE-627)DOAJ053310241 (DE-599)DOAJ2d2a0b7915f944469a8b49515d304c1d DE-627 ger DE-627 rakwb eng QK1-989 Prakash G Pati verfasserin aut Using AFLP-RGA markers to assess genetic diversity among pigeon pea (Cajanus cajan) genotypes in relation to major diseases 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Resistance gene analog (RGA)-anchored amplified fragment length polymorphism (AFLP-RGA) marker system was used in order to evaluate genetic relationships among 22 pigeon pea genotypes with varied responses to Fusarium wilt and sterility mosaic disease. Five AFLP-RGA primer combinations (E-CAG/wlrk-S, M-GTG/wlrk-S, M-GTG/wlrk-AS, E-CAT/S1-INV and E-CAG/wlrk-AS) produced 173 scorable fragments, of which 157 (90.7%) were polymorphic, with an average of 31.4 fragments per primer combination. The polymorphism rates obtained with the five primers were 83.3%, 92.0%, 92.3%, 93.0% and 93.1%, respectively. Mean polymorphic information content (PIC) values ranged from 0.24 (with E-CAT/S1-INV) to 0.30 (with E-CAG/wlrk-AS), whereas resolving power (RP) values varied from 11.06 (with M-GTG/wlrk-S) to 25.51 (with E-CAG/wlrk-AS) and marker index (MI) values ranged from 5.98 (with M-GTG/wlrk-S) to 12.30 (with E-CAG/wlrk-AS). We identified a positive correlation between MI and RP (r²=0.98, p<0.05), stronger that that observed for the comparison between PIC and RP (r²=0.88, p<0.05). That implies that either MI or RP is the best parameter for selecting more informative AFLP-RGA primer combinations. The Jaccard coefficient ranged from 0.07 to 0.72, suggesting a broad genetic base in the genotypes studied. A neighbor-joining tree, based on the unweighted pair group method with arithmetic mean, distinguished cultivated species from wild species. The grouping of resistant genotypes in different clusters would help in the selection of suitable donors for resistance breeding in pigeon pea. AFLP-RGA Cajanus cajan genetic diversity molecular markers polymorphism Botany Subhojit Datta verfasserin aut Ikechukwu O Agbagwa verfasserin aut Indra Prakash Singh verfasserin aut Khela Ram Soren verfasserin aut Alok Das verfasserin aut Arbind Kumar Choudhary verfasserin aut Sushil Kumar Chaturvedi verfasserin aut In Acta Botânica Brasílica Sociedade Botânica do Brasil, 2004 28(2014), 2, Seite 198-205 (DE-627)332926419 (DE-600)2055440-0 1677941X nnns volume:28 year:2014 number:2 pages:198-205 https://doi.org/10.1590/S0102-33062014000200007 kostenfrei https://doaj.org/article/2d2a0b7915f944469a8b49515d304c1d kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000200007&lng=en&tlng=en kostenfrei https://doaj.org/toc/1677-941X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2014 2 198-205
allfields_unstemmed	10.1590/S0102-33062014000200007 doi (DE-627)DOAJ053310241 (DE-599)DOAJ2d2a0b7915f944469a8b49515d304c1d DE-627 ger DE-627 rakwb eng QK1-989 Prakash G Pati verfasserin aut Using AFLP-RGA markers to assess genetic diversity among pigeon pea (Cajanus cajan) genotypes in relation to major diseases 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Resistance gene analog (RGA)-anchored amplified fragment length polymorphism (AFLP-RGA) marker system was used in order to evaluate genetic relationships among 22 pigeon pea genotypes with varied responses to Fusarium wilt and sterility mosaic disease. Five AFLP-RGA primer combinations (E-CAG/wlrk-S, M-GTG/wlrk-S, M-GTG/wlrk-AS, E-CAT/S1-INV and E-CAG/wlrk-AS) produced 173 scorable fragments, of which 157 (90.7%) were polymorphic, with an average of 31.4 fragments per primer combination. The polymorphism rates obtained with the five primers were 83.3%, 92.0%, 92.3%, 93.0% and 93.1%, respectively. Mean polymorphic information content (PIC) values ranged from 0.24 (with E-CAT/S1-INV) to 0.30 (with E-CAG/wlrk-AS), whereas resolving power (RP) values varied from 11.06 (with M-GTG/wlrk-S) to 25.51 (with E-CAG/wlrk-AS) and marker index (MI) values ranged from 5.98 (with M-GTG/wlrk-S) to 12.30 (with E-CAG/wlrk-AS). We identified a positive correlation between MI and RP (r²=0.98, p<0.05), stronger that that observed for the comparison between PIC and RP (r²=0.88, p<0.05). That implies that either MI or RP is the best parameter for selecting more informative AFLP-RGA primer combinations. The Jaccard coefficient ranged from 0.07 to 0.72, suggesting a broad genetic base in the genotypes studied. A neighbor-joining tree, based on the unweighted pair group method with arithmetic mean, distinguished cultivated species from wild species. The grouping of resistant genotypes in different clusters would help in the selection of suitable donors for resistance breeding in pigeon pea. AFLP-RGA Cajanus cajan genetic diversity molecular markers polymorphism Botany Subhojit Datta verfasserin aut Ikechukwu O Agbagwa verfasserin aut Indra Prakash Singh verfasserin aut Khela Ram Soren verfasserin aut Alok Das verfasserin aut Arbind Kumar Choudhary verfasserin aut Sushil Kumar Chaturvedi verfasserin aut In Acta Botânica Brasílica Sociedade Botânica do Brasil, 2004 28(2014), 2, Seite 198-205 (DE-627)332926419 (DE-600)2055440-0 1677941X nnns volume:28 year:2014 number:2 pages:198-205 https://doi.org/10.1590/S0102-33062014000200007 kostenfrei https://doaj.org/article/2d2a0b7915f944469a8b49515d304c1d kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000200007&lng=en&tlng=en kostenfrei https://doaj.org/toc/1677-941X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2014 2 198-205
allfieldsGer	10.1590/S0102-33062014000200007 doi (DE-627)DOAJ053310241 (DE-599)DOAJ2d2a0b7915f944469a8b49515d304c1d DE-627 ger DE-627 rakwb eng QK1-989 Prakash G Pati verfasserin aut Using AFLP-RGA markers to assess genetic diversity among pigeon pea (Cajanus cajan) genotypes in relation to major diseases 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Resistance gene analog (RGA)-anchored amplified fragment length polymorphism (AFLP-RGA) marker system was used in order to evaluate genetic relationships among 22 pigeon pea genotypes with varied responses to Fusarium wilt and sterility mosaic disease. Five AFLP-RGA primer combinations (E-CAG/wlrk-S, M-GTG/wlrk-S, M-GTG/wlrk-AS, E-CAT/S1-INV and E-CAG/wlrk-AS) produced 173 scorable fragments, of which 157 (90.7%) were polymorphic, with an average of 31.4 fragments per primer combination. The polymorphism rates obtained with the five primers were 83.3%, 92.0%, 92.3%, 93.0% and 93.1%, respectively. Mean polymorphic information content (PIC) values ranged from 0.24 (with E-CAT/S1-INV) to 0.30 (with E-CAG/wlrk-AS), whereas resolving power (RP) values varied from 11.06 (with M-GTG/wlrk-S) to 25.51 (with E-CAG/wlrk-AS) and marker index (MI) values ranged from 5.98 (with M-GTG/wlrk-S) to 12.30 (with E-CAG/wlrk-AS). We identified a positive correlation between MI and RP (r²=0.98, p<0.05), stronger that that observed for the comparison between PIC and RP (r²=0.88, p<0.05). That implies that either MI or RP is the best parameter for selecting more informative AFLP-RGA primer combinations. The Jaccard coefficient ranged from 0.07 to 0.72, suggesting a broad genetic base in the genotypes studied. A neighbor-joining tree, based on the unweighted pair group method with arithmetic mean, distinguished cultivated species from wild species. The grouping of resistant genotypes in different clusters would help in the selection of suitable donors for resistance breeding in pigeon pea. AFLP-RGA Cajanus cajan genetic diversity molecular markers polymorphism Botany Subhojit Datta verfasserin aut Ikechukwu O Agbagwa verfasserin aut Indra Prakash Singh verfasserin aut Khela Ram Soren verfasserin aut Alok Das verfasserin aut Arbind Kumar Choudhary verfasserin aut Sushil Kumar Chaturvedi verfasserin aut In Acta Botânica Brasílica Sociedade Botânica do Brasil, 2004 28(2014), 2, Seite 198-205 (DE-627)332926419 (DE-600)2055440-0 1677941X nnns volume:28 year:2014 number:2 pages:198-205 https://doi.org/10.1590/S0102-33062014000200007 kostenfrei https://doaj.org/article/2d2a0b7915f944469a8b49515d304c1d kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000200007&lng=en&tlng=en kostenfrei https://doaj.org/toc/1677-941X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2014 2 198-205
allfieldsSound	10.1590/S0102-33062014000200007 doi (DE-627)DOAJ053310241 (DE-599)DOAJ2d2a0b7915f944469a8b49515d304c1d DE-627 ger DE-627 rakwb eng QK1-989 Prakash G Pati verfasserin aut Using AFLP-RGA markers to assess genetic diversity among pigeon pea (Cajanus cajan) genotypes in relation to major diseases 2014 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Resistance gene analog (RGA)-anchored amplified fragment length polymorphism (AFLP-RGA) marker system was used in order to evaluate genetic relationships among 22 pigeon pea genotypes with varied responses to Fusarium wilt and sterility mosaic disease. Five AFLP-RGA primer combinations (E-CAG/wlrk-S, M-GTG/wlrk-S, M-GTG/wlrk-AS, E-CAT/S1-INV and E-CAG/wlrk-AS) produced 173 scorable fragments, of which 157 (90.7%) were polymorphic, with an average of 31.4 fragments per primer combination. The polymorphism rates obtained with the five primers were 83.3%, 92.0%, 92.3%, 93.0% and 93.1%, respectively. Mean polymorphic information content (PIC) values ranged from 0.24 (with E-CAT/S1-INV) to 0.30 (with E-CAG/wlrk-AS), whereas resolving power (RP) values varied from 11.06 (with M-GTG/wlrk-S) to 25.51 (with E-CAG/wlrk-AS) and marker index (MI) values ranged from 5.98 (with M-GTG/wlrk-S) to 12.30 (with E-CAG/wlrk-AS). We identified a positive correlation between MI and RP (r²=0.98, p<0.05), stronger that that observed for the comparison between PIC and RP (r²=0.88, p<0.05). That implies that either MI or RP is the best parameter for selecting more informative AFLP-RGA primer combinations. The Jaccard coefficient ranged from 0.07 to 0.72, suggesting a broad genetic base in the genotypes studied. A neighbor-joining tree, based on the unweighted pair group method with arithmetic mean, distinguished cultivated species from wild species. The grouping of resistant genotypes in different clusters would help in the selection of suitable donors for resistance breeding in pigeon pea. AFLP-RGA Cajanus cajan genetic diversity molecular markers polymorphism Botany Subhojit Datta verfasserin aut Ikechukwu O Agbagwa verfasserin aut Indra Prakash Singh verfasserin aut Khela Ram Soren verfasserin aut Alok Das verfasserin aut Arbind Kumar Choudhary verfasserin aut Sushil Kumar Chaturvedi verfasserin aut In Acta Botânica Brasílica Sociedade Botânica do Brasil, 2004 28(2014), 2, Seite 198-205 (DE-627)332926419 (DE-600)2055440-0 1677941X nnns volume:28 year:2014 number:2 pages:198-205 https://doi.org/10.1590/S0102-33062014000200007 kostenfrei https://doaj.org/article/2d2a0b7915f944469a8b49515d304c1d kostenfrei http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000200007&lng=en&tlng=en kostenfrei https://doaj.org/toc/1677-941X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 28 2014 2 198-205
language	English
source	In Acta Botânica Brasílica 28(2014), 2, Seite 198-205 volume:28 year:2014 number:2 pages:198-205
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topic_title	QK1-989 Using AFLP-RGA markers to assess genetic diversity among pigeon pea (Cajanus cajan) genotypes in relation to major diseases AFLP-RGA Cajanus cajan genetic diversity molecular markers polymorphism
topic	misc QK1-989 misc AFLP-RGA misc Cajanus cajan misc genetic diversity misc molecular markers misc polymorphism misc Botany
topic_unstemmed	misc QK1-989 misc AFLP-RGA misc Cajanus cajan misc genetic diversity misc molecular markers misc polymorphism misc Botany
topic_browse	misc QK1-989 misc AFLP-RGA misc Cajanus cajan misc genetic diversity misc molecular markers misc polymorphism misc Botany
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title	Using AFLP-RGA markers to assess genetic diversity among pigeon pea (Cajanus cajan) genotypes in relation to major diseases
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title_full	Using AFLP-RGA markers to assess genetic diversity among pigeon pea (Cajanus cajan) genotypes in relation to major diseases
author_sort	Prakash G Pati
journal	Acta Botânica Brasílica
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author_browse	Prakash G Pati Subhojit Datta Ikechukwu O Agbagwa Indra Prakash Singh Khela Ram Soren Alok Das Arbind Kumar Choudhary Sushil Kumar Chaturvedi
container_volume	28
class	QK1-989
format_se	Elektronische Aufsätze
author-letter	Prakash G Pati
doi_str_mv	10.1590/S0102-33062014000200007
author2-role	verfasserin
title_sort	using aflp-rga markers to assess genetic diversity among pigeon pea (cajanus cajan) genotypes in relation to major diseases
callnumber	QK1-989
title_auth	Using AFLP-RGA markers to assess genetic diversity among pigeon pea (Cajanus cajan) genotypes in relation to major diseases
abstract	Resistance gene analog (RGA)-anchored amplified fragment length polymorphism (AFLP-RGA) marker system was used in order to evaluate genetic relationships among 22 pigeon pea genotypes with varied responses to Fusarium wilt and sterility mosaic disease. Five AFLP-RGA primer combinations (E-CAG/wlrk-S, M-GTG/wlrk-S, M-GTG/wlrk-AS, E-CAT/S1-INV and E-CAG/wlrk-AS) produced 173 scorable fragments, of which 157 (90.7%) were polymorphic, with an average of 31.4 fragments per primer combination. The polymorphism rates obtained with the five primers were 83.3%, 92.0%, 92.3%, 93.0% and 93.1%, respectively. Mean polymorphic information content (PIC) values ranged from 0.24 (with E-CAT/S1-INV) to 0.30 (with E-CAG/wlrk-AS), whereas resolving power (RP) values varied from 11.06 (with M-GTG/wlrk-S) to 25.51 (with E-CAG/wlrk-AS) and marker index (MI) values ranged from 5.98 (with M-GTG/wlrk-S) to 12.30 (with E-CAG/wlrk-AS). We identified a positive correlation between MI and RP (r²=0.98, p<0.05), stronger that that observed for the comparison between PIC and RP (r²=0.88, p<0.05). That implies that either MI or RP is the best parameter for selecting more informative AFLP-RGA primer combinations. The Jaccard coefficient ranged from 0.07 to 0.72, suggesting a broad genetic base in the genotypes studied. A neighbor-joining tree, based on the unweighted pair group method with arithmetic mean, distinguished cultivated species from wild species. The grouping of resistant genotypes in different clusters would help in the selection of suitable donors for resistance breeding in pigeon pea.
abstractGer	Resistance gene analog (RGA)-anchored amplified fragment length polymorphism (AFLP-RGA) marker system was used in order to evaluate genetic relationships among 22 pigeon pea genotypes with varied responses to Fusarium wilt and sterility mosaic disease. Five AFLP-RGA primer combinations (E-CAG/wlrk-S, M-GTG/wlrk-S, M-GTG/wlrk-AS, E-CAT/S1-INV and E-CAG/wlrk-AS) produced 173 scorable fragments, of which 157 (90.7%) were polymorphic, with an average of 31.4 fragments per primer combination. The polymorphism rates obtained with the five primers were 83.3%, 92.0%, 92.3%, 93.0% and 93.1%, respectively. Mean polymorphic information content (PIC) values ranged from 0.24 (with E-CAT/S1-INV) to 0.30 (with E-CAG/wlrk-AS), whereas resolving power (RP) values varied from 11.06 (with M-GTG/wlrk-S) to 25.51 (with E-CAG/wlrk-AS) and marker index (MI) values ranged from 5.98 (with M-GTG/wlrk-S) to 12.30 (with E-CAG/wlrk-AS). We identified a positive correlation between MI and RP (r²=0.98, p<0.05), stronger that that observed for the comparison between PIC and RP (r²=0.88, p<0.05). That implies that either MI or RP is the best parameter for selecting more informative AFLP-RGA primer combinations. The Jaccard coefficient ranged from 0.07 to 0.72, suggesting a broad genetic base in the genotypes studied. A neighbor-joining tree, based on the unweighted pair group method with arithmetic mean, distinguished cultivated species from wild species. The grouping of resistant genotypes in different clusters would help in the selection of suitable donors for resistance breeding in pigeon pea.
abstract_unstemmed	Resistance gene analog (RGA)-anchored amplified fragment length polymorphism (AFLP-RGA) marker system was used in order to evaluate genetic relationships among 22 pigeon pea genotypes with varied responses to Fusarium wilt and sterility mosaic disease. Five AFLP-RGA primer combinations (E-CAG/wlrk-S, M-GTG/wlrk-S, M-GTG/wlrk-AS, E-CAT/S1-INV and E-CAG/wlrk-AS) produced 173 scorable fragments, of which 157 (90.7%) were polymorphic, with an average of 31.4 fragments per primer combination. The polymorphism rates obtained with the five primers were 83.3%, 92.0%, 92.3%, 93.0% and 93.1%, respectively. Mean polymorphic information content (PIC) values ranged from 0.24 (with E-CAT/S1-INV) to 0.30 (with E-CAG/wlrk-AS), whereas resolving power (RP) values varied from 11.06 (with M-GTG/wlrk-S) to 25.51 (with E-CAG/wlrk-AS) and marker index (MI) values ranged from 5.98 (with M-GTG/wlrk-S) to 12.30 (with E-CAG/wlrk-AS). We identified a positive correlation between MI and RP (r²=0.98, p<0.05), stronger that that observed for the comparison between PIC and RP (r²=0.88, p<0.05). That implies that either MI or RP is the best parameter for selecting more informative AFLP-RGA primer combinations. The Jaccard coefficient ranged from 0.07 to 0.72, suggesting a broad genetic base in the genotypes studied. A neighbor-joining tree, based on the unweighted pair group method with arithmetic mean, distinguished cultivated species from wild species. The grouping of resistant genotypes in different clusters would help in the selection of suitable donors for resistance breeding in pigeon pea.
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title_short	Using AFLP-RGA markers to assess genetic diversity among pigeon pea (Cajanus cajan) genotypes in relation to major diseases
url	https://doi.org/10.1590/S0102-33062014000200007 https://doaj.org/article/2d2a0b7915f944469a8b49515d304c1d http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000200007&lng=en&tlng=en https://doaj.org/toc/1677-941X
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author2	Subhojit Datta Ikechukwu O Agbagwa Indra Prakash Singh Khela Ram Soren Alok Das Arbind Kumar Choudhary Sushil Kumar Chaturvedi
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up_date	2024-07-03T16:57:55.130Z
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