Arabidopsis TCP Transcription Factors Interact with the SUMO Conjugating Machinery in Nuclear Foci

In Arabidopsis more than 400 proteins have been identified as SUMO targets, both in vivo and in vitro. Among others, transcription factors (TFs) are common targets for SUMO conjugation. Here we aimed to exhaustively screen for TFs that interact with the SUMO machinery using an arrayed yeast two-hybr...
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Autor*in:	Magdalena J. Mazur [verfasserIn] Benjamin J. Spears [verfasserIn] André Djajasaputra [verfasserIn] Michelle van der Gragt [verfasserIn] Georgios Vlachakis [verfasserIn] Bas Beerens [verfasserIn] Walter Gassmann [verfasserIn] Harrold A. van den Burg [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2017

Schlagwörter:	SUMO TCP transcription factors SUMO conjugation yeast two-hybrid

Übergeordnetes Werk:	In: Frontiers in Plant Science - Frontiers Media S.A., 2011, 8(2017)
Übergeordnetes Werk:	volume:8 ; year:2017

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3389/fpls.2017.02043

Katalog-ID:	DOAJ053377672

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allfieldsSound	10.3389/fpls.2017.02043 doi (DE-627)DOAJ053377672 (DE-599)DOAJd39ff8b70c394431bdbedf780d66dcb6 DE-627 ger DE-627 rakwb eng SB1-1110 Magdalena J. Mazur verfasserin aut Arabidopsis TCP Transcription Factors Interact with the SUMO Conjugating Machinery in Nuclear Foci 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier In Arabidopsis more than 400 proteins have been identified as SUMO targets, both in vivo and in vitro. Among others, transcription factors (TFs) are common targets for SUMO conjugation. Here we aimed to exhaustively screen for TFs that interact with the SUMO machinery using an arrayed yeast two-hybrid library containing more than 1,100 TFs. We identified 76 interactors that foremost interact with the SUMO conjugation enzyme SCE1 and/or the SUMO E3 ligase SIZ1. These interactors belong to various TF families, which control a wide range of processes in plant development and stress signaling. Amongst these interactors, the TCP family was overrepresented with several TCPs interacting with different proteins of the SUMO conjugation cycle. For a subset of these TCPs we confirmed that the catalytic site of SCE1 is essential for this interaction. In agreement, TCP1, TCP3, TCP8, TCP14, and TCP15 were readily SUMO modified in an E. coli sumoylation assay. Strikingly, these TCP-SCE1 interactions were found to redistribute these TCPs into nuclear foci/speckles, suggesting that these TCP foci represent sites for SUMO (conjugation) activity. SUMO TCP transcription factors SUMO conjugation yeast two-hybrid Plant culture Benjamin J. Spears verfasserin aut André Djajasaputra verfasserin aut Michelle van der Gragt verfasserin aut Georgios Vlachakis verfasserin aut Bas Beerens verfasserin aut Walter Gassmann verfasserin aut Harrold A. van den Burg verfasserin aut In Frontiers in Plant Science Frontiers Media S.A., 2011 8(2017) (DE-627)662359240 (DE-600)2613694-6 1664462X nnns volume:8 year:2017 https://doi.org/10.3389/fpls.2017.02043 kostenfrei https://doaj.org/article/d39ff8b70c394431bdbedf780d66dcb6 kostenfrei http://journal.frontiersin.org/article/10.3389/fpls.2017.02043/full kostenfrei https://doaj.org/toc/1664-462X Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 8 2017
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container_title	Frontiers in Plant Science
authorswithroles_txt_mv	Magdalena J. Mazur @@aut@@ Benjamin J. Spears @@aut@@ André Djajasaputra @@aut@@ Michelle van der Gragt @@aut@@ Georgios Vlachakis @@aut@@ Bas Beerens @@aut@@ Walter Gassmann @@aut@@ Harrold A. van den Burg @@aut@@
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callnumber-first	S - Agriculture
author	Magdalena J. Mazur
spellingShingle	Magdalena J. Mazur misc SB1-1110 misc SUMO misc TCP misc transcription factors misc SUMO conjugation misc yeast two-hybrid misc Plant culture Arabidopsis TCP Transcription Factors Interact with the SUMO Conjugating Machinery in Nuclear Foci
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topic_title	SB1-1110 Arabidopsis TCP Transcription Factors Interact with the SUMO Conjugating Machinery in Nuclear Foci SUMO TCP transcription factors SUMO conjugation yeast two-hybrid
topic	misc SB1-1110 misc SUMO misc TCP misc transcription factors misc SUMO conjugation misc yeast two-hybrid misc Plant culture
topic_unstemmed	misc SB1-1110 misc SUMO misc TCP misc transcription factors misc SUMO conjugation misc yeast two-hybrid misc Plant culture
topic_browse	misc SB1-1110 misc SUMO misc TCP misc transcription factors misc SUMO conjugation misc yeast two-hybrid misc Plant culture
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title	Arabidopsis TCP Transcription Factors Interact with the SUMO Conjugating Machinery in Nuclear Foci
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title_full	Arabidopsis TCP Transcription Factors Interact with the SUMO Conjugating Machinery in Nuclear Foci
author_sort	Magdalena J. Mazur
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author_browse	Magdalena J. Mazur Benjamin J. Spears André Djajasaputra Michelle van der Gragt Georgios Vlachakis Bas Beerens Walter Gassmann Harrold A. van den Burg
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title_sort	arabidopsis tcp transcription factors interact with the sumo conjugating machinery in nuclear foci
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title_auth	Arabidopsis TCP Transcription Factors Interact with the SUMO Conjugating Machinery in Nuclear Foci
abstract	In Arabidopsis more than 400 proteins have been identified as SUMO targets, both in vivo and in vitro. Among others, transcription factors (TFs) are common targets for SUMO conjugation. Here we aimed to exhaustively screen for TFs that interact with the SUMO machinery using an arrayed yeast two-hybrid library containing more than 1,100 TFs. We identified 76 interactors that foremost interact with the SUMO conjugation enzyme SCE1 and/or the SUMO E3 ligase SIZ1. These interactors belong to various TF families, which control a wide range of processes in plant development and stress signaling. Amongst these interactors, the TCP family was overrepresented with several TCPs interacting with different proteins of the SUMO conjugation cycle. For a subset of these TCPs we confirmed that the catalytic site of SCE1 is essential for this interaction. In agreement, TCP1, TCP3, TCP8, TCP14, and TCP15 were readily SUMO modified in an E. coli sumoylation assay. Strikingly, these TCP-SCE1 interactions were found to redistribute these TCPs into nuclear foci/speckles, suggesting that these TCP foci represent sites for SUMO (conjugation) activity.
abstractGer	In Arabidopsis more than 400 proteins have been identified as SUMO targets, both in vivo and in vitro. Among others, transcription factors (TFs) are common targets for SUMO conjugation. Here we aimed to exhaustively screen for TFs that interact with the SUMO machinery using an arrayed yeast two-hybrid library containing more than 1,100 TFs. We identified 76 interactors that foremost interact with the SUMO conjugation enzyme SCE1 and/or the SUMO E3 ligase SIZ1. These interactors belong to various TF families, which control a wide range of processes in plant development and stress signaling. Amongst these interactors, the TCP family was overrepresented with several TCPs interacting with different proteins of the SUMO conjugation cycle. For a subset of these TCPs we confirmed that the catalytic site of SCE1 is essential for this interaction. In agreement, TCP1, TCP3, TCP8, TCP14, and TCP15 were readily SUMO modified in an E. coli sumoylation assay. Strikingly, these TCP-SCE1 interactions were found to redistribute these TCPs into nuclear foci/speckles, suggesting that these TCP foci represent sites for SUMO (conjugation) activity.
abstract_unstemmed	In Arabidopsis more than 400 proteins have been identified as SUMO targets, both in vivo and in vitro. Among others, transcription factors (TFs) are common targets for SUMO conjugation. Here we aimed to exhaustively screen for TFs that interact with the SUMO machinery using an arrayed yeast two-hybrid library containing more than 1,100 TFs. We identified 76 interactors that foremost interact with the SUMO conjugation enzyme SCE1 and/or the SUMO E3 ligase SIZ1. These interactors belong to various TF families, which control a wide range of processes in plant development and stress signaling. Amongst these interactors, the TCP family was overrepresented with several TCPs interacting with different proteins of the SUMO conjugation cycle. For a subset of these TCPs we confirmed that the catalytic site of SCE1 is essential for this interaction. In agreement, TCP1, TCP3, TCP8, TCP14, and TCP15 were readily SUMO modified in an E. coli sumoylation assay. Strikingly, these TCP-SCE1 interactions were found to redistribute these TCPs into nuclear foci/speckles, suggesting that these TCP foci represent sites for SUMO (conjugation) activity.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
title_short	Arabidopsis TCP Transcription Factors Interact with the SUMO Conjugating Machinery in Nuclear Foci
url	https://doi.org/10.3389/fpls.2017.02043 https://doaj.org/article/d39ff8b70c394431bdbedf780d66dcb6 http://journal.frontiersin.org/article/10.3389/fpls.2017.02043/full https://doaj.org/toc/1664-462X
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