Chromatin immunoprecipitation of transcription factors and histone modifications in Comma-Dβ mammary epithelial cells

Summary: Chromatin immunoprecipitation (ChIP) is used to study interactions between proteins and DNA. Nuclear lysates are prepared, and chromatin is fragmented by sonication. Antibodies are used to purify a protein of interest (e.g., a transcription factor or histone mark) along with any bound DNA....
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Holly Holliday [verfasserIn] Amanda Khoury [verfasserIn] Alexander Swarbrick [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	ChIP-seq Chromatin immunoprecipitation (ChIP) Molecular Biology

Übergeordnetes Werk:	In: STAR Protocols - Elsevier, 2020, 2(2021), 2, Seite 100514-
Übergeordnetes Werk:	volume:2 ; year:2021 ; number:2 ; pages:100514-

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.xpro.2021.100514

Katalog-ID:	DOAJ053457609

Internformat


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allfields	10.1016/j.xpro.2021.100514 doi (DE-627)DOAJ053457609 (DE-599)DOAJa373610bcb264f5bbd872dc0303e33b6 DE-627 ger DE-627 rakwb eng Q1-390 Holly Holliday verfasserin aut Chromatin immunoprecipitation of transcription factors and histone modifications in Comma-Dβ mammary epithelial cells 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Chromatin immunoprecipitation (ChIP) is used to study interactions between proteins and DNA. Nuclear lysates are prepared, and chromatin is fragmented by sonication. Antibodies are used to purify a protein of interest (e.g., a transcription factor or histone mark) along with any bound DNA. The genomic binding sites can then be mapped by sequencing the bound DNA (ChIP-seq) or by qPCR if binding sites are already known. ChIP requires optimization for each cell type, and success is highly antibody dependent. This protocol can be adapted to other cell lines with careful optimization.For complete details on the use and execution of this protocol, please refer to Holliday et al. (2021). ChIP-seq Chromatin immunoprecipitation (ChIP) Molecular Biology Science (General) Amanda Khoury verfasserin aut Alexander Swarbrick verfasserin aut In STAR Protocols Elsevier, 2020 2(2021), 2, Seite 100514- (DE-627)1747970557 26661667 nnns volume:2 year:2021 number:2 pages:100514- https://doi.org/10.1016/j.xpro.2021.100514 kostenfrei https://doaj.org/article/a373610bcb264f5bbd872dc0303e33b6 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166721002215 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 2 2021 2 100514-
spelling	10.1016/j.xpro.2021.100514 doi (DE-627)DOAJ053457609 (DE-599)DOAJa373610bcb264f5bbd872dc0303e33b6 DE-627 ger DE-627 rakwb eng Q1-390 Holly Holliday verfasserin aut Chromatin immunoprecipitation of transcription factors and histone modifications in Comma-Dβ mammary epithelial cells 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Chromatin immunoprecipitation (ChIP) is used to study interactions between proteins and DNA. Nuclear lysates are prepared, and chromatin is fragmented by sonication. Antibodies are used to purify a protein of interest (e.g., a transcription factor or histone mark) along with any bound DNA. The genomic binding sites can then be mapped by sequencing the bound DNA (ChIP-seq) or by qPCR if binding sites are already known. ChIP requires optimization for each cell type, and success is highly antibody dependent. This protocol can be adapted to other cell lines with careful optimization.For complete details on the use and execution of this protocol, please refer to Holliday et al. (2021). ChIP-seq Chromatin immunoprecipitation (ChIP) Molecular Biology Science (General) Amanda Khoury verfasserin aut Alexander Swarbrick verfasserin aut In STAR Protocols Elsevier, 2020 2(2021), 2, Seite 100514- (DE-627)1747970557 26661667 nnns volume:2 year:2021 number:2 pages:100514- https://doi.org/10.1016/j.xpro.2021.100514 kostenfrei https://doaj.org/article/a373610bcb264f5bbd872dc0303e33b6 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166721002215 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 2 2021 2 100514-
allfields_unstemmed	10.1016/j.xpro.2021.100514 doi (DE-627)DOAJ053457609 (DE-599)DOAJa373610bcb264f5bbd872dc0303e33b6 DE-627 ger DE-627 rakwb eng Q1-390 Holly Holliday verfasserin aut Chromatin immunoprecipitation of transcription factors and histone modifications in Comma-Dβ mammary epithelial cells 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Chromatin immunoprecipitation (ChIP) is used to study interactions between proteins and DNA. Nuclear lysates are prepared, and chromatin is fragmented by sonication. Antibodies are used to purify a protein of interest (e.g., a transcription factor or histone mark) along with any bound DNA. The genomic binding sites can then be mapped by sequencing the bound DNA (ChIP-seq) or by qPCR if binding sites are already known. ChIP requires optimization for each cell type, and success is highly antibody dependent. This protocol can be adapted to other cell lines with careful optimization.For complete details on the use and execution of this protocol, please refer to Holliday et al. (2021). ChIP-seq Chromatin immunoprecipitation (ChIP) Molecular Biology Science (General) Amanda Khoury verfasserin aut Alexander Swarbrick verfasserin aut In STAR Protocols Elsevier, 2020 2(2021), 2, Seite 100514- (DE-627)1747970557 26661667 nnns volume:2 year:2021 number:2 pages:100514- https://doi.org/10.1016/j.xpro.2021.100514 kostenfrei https://doaj.org/article/a373610bcb264f5bbd872dc0303e33b6 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166721002215 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 2 2021 2 100514-
allfieldsGer	10.1016/j.xpro.2021.100514 doi (DE-627)DOAJ053457609 (DE-599)DOAJa373610bcb264f5bbd872dc0303e33b6 DE-627 ger DE-627 rakwb eng Q1-390 Holly Holliday verfasserin aut Chromatin immunoprecipitation of transcription factors and histone modifications in Comma-Dβ mammary epithelial cells 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Chromatin immunoprecipitation (ChIP) is used to study interactions between proteins and DNA. Nuclear lysates are prepared, and chromatin is fragmented by sonication. Antibodies are used to purify a protein of interest (e.g., a transcription factor or histone mark) along with any bound DNA. The genomic binding sites can then be mapped by sequencing the bound DNA (ChIP-seq) or by qPCR if binding sites are already known. ChIP requires optimization for each cell type, and success is highly antibody dependent. This protocol can be adapted to other cell lines with careful optimization.For complete details on the use and execution of this protocol, please refer to Holliday et al. (2021). ChIP-seq Chromatin immunoprecipitation (ChIP) Molecular Biology Science (General) Amanda Khoury verfasserin aut Alexander Swarbrick verfasserin aut In STAR Protocols Elsevier, 2020 2(2021), 2, Seite 100514- (DE-627)1747970557 26661667 nnns volume:2 year:2021 number:2 pages:100514- https://doi.org/10.1016/j.xpro.2021.100514 kostenfrei https://doaj.org/article/a373610bcb264f5bbd872dc0303e33b6 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166721002215 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 2 2021 2 100514-
allfieldsSound	10.1016/j.xpro.2021.100514 doi (DE-627)DOAJ053457609 (DE-599)DOAJa373610bcb264f5bbd872dc0303e33b6 DE-627 ger DE-627 rakwb eng Q1-390 Holly Holliday verfasserin aut Chromatin immunoprecipitation of transcription factors and histone modifications in Comma-Dβ mammary epithelial cells 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Summary: Chromatin immunoprecipitation (ChIP) is used to study interactions between proteins and DNA. Nuclear lysates are prepared, and chromatin is fragmented by sonication. Antibodies are used to purify a protein of interest (e.g., a transcription factor or histone mark) along with any bound DNA. The genomic binding sites can then be mapped by sequencing the bound DNA (ChIP-seq) or by qPCR if binding sites are already known. ChIP requires optimization for each cell type, and success is highly antibody dependent. This protocol can be adapted to other cell lines with careful optimization.For complete details on the use and execution of this protocol, please refer to Holliday et al. (2021). ChIP-seq Chromatin immunoprecipitation (ChIP) Molecular Biology Science (General) Amanda Khoury verfasserin aut Alexander Swarbrick verfasserin aut In STAR Protocols Elsevier, 2020 2(2021), 2, Seite 100514- (DE-627)1747970557 26661667 nnns volume:2 year:2021 number:2 pages:100514- https://doi.org/10.1016/j.xpro.2021.100514 kostenfrei https://doaj.org/article/a373610bcb264f5bbd872dc0303e33b6 kostenfrei http://www.sciencedirect.com/science/article/pii/S2666166721002215 kostenfrei https://doaj.org/toc/2666-1667 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2110 GBV_ILN_2112 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 2 2021 2 100514-
language	English
source	In STAR Protocols 2(2021), 2, Seite 100514- volume:2 year:2021 number:2 pages:100514-
sourceStr	In STAR Protocols 2(2021), 2, Seite 100514- volume:2 year:2021 number:2 pages:100514-
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	ChIP-seq Chromatin immunoprecipitation (ChIP) Molecular Biology Science (General)
isfreeaccess_bool	true
container_title	STAR Protocols
authorswithroles_txt_mv	Holly Holliday @@aut@@ Amanda Khoury @@aut@@ Alexander Swarbrick @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	1747970557
id	DOAJ053457609
language_de	englisch
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callnumber-first	Q - Science
author	Holly Holliday
spellingShingle	Holly Holliday misc Q1-390 misc ChIP-seq misc Chromatin immunoprecipitation (ChIP) misc Molecular Biology misc Science (General) Chromatin immunoprecipitation of transcription factors and histone modifications in Comma-Dβ mammary epithelial cells
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topic_title	Q1-390 Chromatin immunoprecipitation of transcription factors and histone modifications in Comma-Dβ mammary epithelial cells ChIP-seq Chromatin immunoprecipitation (ChIP) Molecular Biology
topic	misc Q1-390 misc ChIP-seq misc Chromatin immunoprecipitation (ChIP) misc Molecular Biology misc Science (General)
topic_unstemmed	misc Q1-390 misc ChIP-seq misc Chromatin immunoprecipitation (ChIP) misc Molecular Biology misc Science (General)
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title	Chromatin immunoprecipitation of transcription factors and histone modifications in Comma-Dβ mammary epithelial cells
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title_full	Chromatin immunoprecipitation of transcription factors and histone modifications in Comma-Dβ mammary epithelial cells
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title_sort	chromatin immunoprecipitation of transcription factors and histone modifications in comma-dβ mammary epithelial cells
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title_auth	Chromatin immunoprecipitation of transcription factors and histone modifications in Comma-Dβ mammary epithelial cells
abstract	Summary: Chromatin immunoprecipitation (ChIP) is used to study interactions between proteins and DNA. Nuclear lysates are prepared, and chromatin is fragmented by sonication. Antibodies are used to purify a protein of interest (e.g., a transcription factor or histone mark) along with any bound DNA. The genomic binding sites can then be mapped by sequencing the bound DNA (ChIP-seq) or by qPCR if binding sites are already known. ChIP requires optimization for each cell type, and success is highly antibody dependent. This protocol can be adapted to other cell lines with careful optimization.For complete details on the use and execution of this protocol, please refer to Holliday et al. (2021).
abstractGer	Summary: Chromatin immunoprecipitation (ChIP) is used to study interactions between proteins and DNA. Nuclear lysates are prepared, and chromatin is fragmented by sonication. Antibodies are used to purify a protein of interest (e.g., a transcription factor or histone mark) along with any bound DNA. The genomic binding sites can then be mapped by sequencing the bound DNA (ChIP-seq) or by qPCR if binding sites are already known. ChIP requires optimization for each cell type, and success is highly antibody dependent. This protocol can be adapted to other cell lines with careful optimization.For complete details on the use and execution of this protocol, please refer to Holliday et al. (2021).
abstract_unstemmed	Summary: Chromatin immunoprecipitation (ChIP) is used to study interactions between proteins and DNA. Nuclear lysates are prepared, and chromatin is fragmented by sonication. Antibodies are used to purify a protein of interest (e.g., a transcription factor or histone mark) along with any bound DNA. The genomic binding sites can then be mapped by sequencing the bound DNA (ChIP-seq) or by qPCR if binding sites are already known. ChIP requires optimization for each cell type, and success is highly antibody dependent. This protocol can be adapted to other cell lines with careful optimization.For complete details on the use and execution of this protocol, please refer to Holliday et al. (2021).
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title_short	Chromatin immunoprecipitation of transcription factors and histone modifications in Comma-Dβ mammary epithelial cells
url	https://doi.org/10.1016/j.xpro.2021.100514 https://doaj.org/article/a373610bcb264f5bbd872dc0303e33b6 http://www.sciencedirect.com/science/article/pii/S2666166721002215 https://doaj.org/toc/2666-1667
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up_date	2024-07-03T17:46:18.397Z
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Chromatin immunoprecipitation of transcription factors and histone modifications in Comma-Dβ mammary epithelial cells

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