A comparative study of three tissue-cultured Dendrobium species and their wild correspondences by headspace gas chromatography–mass spectrometry combined with chemometric methods

Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences. In this paper, a headspace gas chromatogr...
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Autor*in:	Nai-Dong Chen [verfasserIn] Tao You [verfasserIn] Jun Li [verfasserIn] Li-Tao Bai [verfasserIn] Jing-Wen Hao [verfasserIn] Xiao-Yuan Xu [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	Dendrobium huoshanense Dendrobium moniliforme Dendrobium officinale gas chromatography–mass spectrometry principal component analysis

Übergeordnetes Werk:	In: Journal of Food and Drug Analysis - Elsevier, 2016, 24(2016), 4, Seite 839-847
Übergeordnetes Werk:	volume:24 ; year:2016 ; number:4 ; pages:839-847

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.1016/j.jfda.2016.05.006

Katalog-ID:	DOAJ053654862

Internformat


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spelling	10.1016/j.jfda.2016.05.006 doi (DE-627)DOAJ053654862 (DE-599)DOAJaa83e95e297741cbb422f4228a71a286 DE-627 ger DE-627 rakwb eng TX341-641 RM1-950 Nai-Dong Chen verfasserin aut A comparative study of three tissue-cultured Dendrobium species and their wild correspondences by headspace gas chromatography–mass spectrometry combined with chemometric methods 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences. In this paper, a headspace gas chromatography–mass spectrometry method combined with chemometric methods was applied to analyze and evaluate the volatile compounds in tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme (Linn.) Sw. In total, 63 volatile compounds were separated, with 53 being identified from the three Dendrobium spp. samples. Different provenances of Dendrobiums had characteristic chemicals and showed remarkable quantity discrepancy of common compositions. The similarity evaluation disclosed that the accumulation of volatile compounds in Dendrobium samples might be affected by their provenance. Principal component analysis showed that the first three components explained 85.9% of data variance, demonstrating a good discrimination between samples. Gas chromatography–mass spectrometry techniques, combined with chemometrics, might be an effective strategy for identifying the species and their provenance, especially in the assessment of tissue-cultured Dendrobium quality for use in raw herbal medicines. Dendrobium huoshanense Dendrobium moniliforme Dendrobium officinale gas chromatography–mass spectrometry principal component analysis Nutrition. Foods and food supply Therapeutics. Pharmacology Tao You verfasserin aut Jun Li verfasserin aut Li-Tao Bai verfasserin aut Jing-Wen Hao verfasserin aut Xiao-Yuan Xu verfasserin aut In Journal of Food and Drug Analysis Elsevier, 2016 24(2016), 4, Seite 839-847 (DE-627)363771182 (DE-600)2108571-7 10219498 nnns volume:24 year:2016 number:4 pages:839-847 https://doi.org/10.1016/j.jfda.2016.05.006 kostenfrei https://doaj.org/article/aa83e95e297741cbb422f4228a71a286 kostenfrei http://www.sciencedirect.com/science/article/pii/S1021949816300771 kostenfrei https://doaj.org/toc/1021-9498 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2016 4 839-847
allfields_unstemmed	10.1016/j.jfda.2016.05.006 doi (DE-627)DOAJ053654862 (DE-599)DOAJaa83e95e297741cbb422f4228a71a286 DE-627 ger DE-627 rakwb eng TX341-641 RM1-950 Nai-Dong Chen verfasserin aut A comparative study of three tissue-cultured Dendrobium species and their wild correspondences by headspace gas chromatography–mass spectrometry combined with chemometric methods 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences. In this paper, a headspace gas chromatography–mass spectrometry method combined with chemometric methods was applied to analyze and evaluate the volatile compounds in tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme (Linn.) Sw. In total, 63 volatile compounds were separated, with 53 being identified from the three Dendrobium spp. samples. Different provenances of Dendrobiums had characteristic chemicals and showed remarkable quantity discrepancy of common compositions. The similarity evaluation disclosed that the accumulation of volatile compounds in Dendrobium samples might be affected by their provenance. Principal component analysis showed that the first three components explained 85.9% of data variance, demonstrating a good discrimination between samples. Gas chromatography–mass spectrometry techniques, combined with chemometrics, might be an effective strategy for identifying the species and their provenance, especially in the assessment of tissue-cultured Dendrobium quality for use in raw herbal medicines. Dendrobium huoshanense Dendrobium moniliforme Dendrobium officinale gas chromatography–mass spectrometry principal component analysis Nutrition. Foods and food supply Therapeutics. Pharmacology Tao You verfasserin aut Jun Li verfasserin aut Li-Tao Bai verfasserin aut Jing-Wen Hao verfasserin aut Xiao-Yuan Xu verfasserin aut In Journal of Food and Drug Analysis Elsevier, 2016 24(2016), 4, Seite 839-847 (DE-627)363771182 (DE-600)2108571-7 10219498 nnns volume:24 year:2016 number:4 pages:839-847 https://doi.org/10.1016/j.jfda.2016.05.006 kostenfrei https://doaj.org/article/aa83e95e297741cbb422f4228a71a286 kostenfrei http://www.sciencedirect.com/science/article/pii/S1021949816300771 kostenfrei https://doaj.org/toc/1021-9498 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2016 4 839-847
allfieldsGer	10.1016/j.jfda.2016.05.006 doi (DE-627)DOAJ053654862 (DE-599)DOAJaa83e95e297741cbb422f4228a71a286 DE-627 ger DE-627 rakwb eng TX341-641 RM1-950 Nai-Dong Chen verfasserin aut A comparative study of three tissue-cultured Dendrobium species and their wild correspondences by headspace gas chromatography–mass spectrometry combined with chemometric methods 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences. In this paper, a headspace gas chromatography–mass spectrometry method combined with chemometric methods was applied to analyze and evaluate the volatile compounds in tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme (Linn.) Sw. In total, 63 volatile compounds were separated, with 53 being identified from the three Dendrobium spp. samples. Different provenances of Dendrobiums had characteristic chemicals and showed remarkable quantity discrepancy of common compositions. The similarity evaluation disclosed that the accumulation of volatile compounds in Dendrobium samples might be affected by their provenance. Principal component analysis showed that the first three components explained 85.9% of data variance, demonstrating a good discrimination between samples. Gas chromatography–mass spectrometry techniques, combined with chemometrics, might be an effective strategy for identifying the species and their provenance, especially in the assessment of tissue-cultured Dendrobium quality for use in raw herbal medicines. Dendrobium huoshanense Dendrobium moniliforme Dendrobium officinale gas chromatography–mass spectrometry principal component analysis Nutrition. Foods and food supply Therapeutics. Pharmacology Tao You verfasserin aut Jun Li verfasserin aut Li-Tao Bai verfasserin aut Jing-Wen Hao verfasserin aut Xiao-Yuan Xu verfasserin aut In Journal of Food and Drug Analysis Elsevier, 2016 24(2016), 4, Seite 839-847 (DE-627)363771182 (DE-600)2108571-7 10219498 nnns volume:24 year:2016 number:4 pages:839-847 https://doi.org/10.1016/j.jfda.2016.05.006 kostenfrei https://doaj.org/article/aa83e95e297741cbb422f4228a71a286 kostenfrei http://www.sciencedirect.com/science/article/pii/S1021949816300771 kostenfrei https://doaj.org/toc/1021-9498 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2016 4 839-847
allfieldsSound	10.1016/j.jfda.2016.05.006 doi (DE-627)DOAJ053654862 (DE-599)DOAJaa83e95e297741cbb422f4228a71a286 DE-627 ger DE-627 rakwb eng TX341-641 RM1-950 Nai-Dong Chen verfasserin aut A comparative study of three tissue-cultured Dendrobium species and their wild correspondences by headspace gas chromatography–mass spectrometry combined with chemometric methods 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences. In this paper, a headspace gas chromatography–mass spectrometry method combined with chemometric methods was applied to analyze and evaluate the volatile compounds in tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme (Linn.) Sw. In total, 63 volatile compounds were separated, with 53 being identified from the three Dendrobium spp. samples. Different provenances of Dendrobiums had characteristic chemicals and showed remarkable quantity discrepancy of common compositions. The similarity evaluation disclosed that the accumulation of volatile compounds in Dendrobium samples might be affected by their provenance. Principal component analysis showed that the first three components explained 85.9% of data variance, demonstrating a good discrimination between samples. Gas chromatography–mass spectrometry techniques, combined with chemometrics, might be an effective strategy for identifying the species and their provenance, especially in the assessment of tissue-cultured Dendrobium quality for use in raw herbal medicines. Dendrobium huoshanense Dendrobium moniliforme Dendrobium officinale gas chromatography–mass spectrometry principal component analysis Nutrition. Foods and food supply Therapeutics. Pharmacology Tao You verfasserin aut Jun Li verfasserin aut Li-Tao Bai verfasserin aut Jing-Wen Hao verfasserin aut Xiao-Yuan Xu verfasserin aut In Journal of Food and Drug Analysis Elsevier, 2016 24(2016), 4, Seite 839-847 (DE-627)363771182 (DE-600)2108571-7 10219498 nnns volume:24 year:2016 number:4 pages:839-847 https://doi.org/10.1016/j.jfda.2016.05.006 kostenfrei https://doaj.org/article/aa83e95e297741cbb422f4228a71a286 kostenfrei http://www.sciencedirect.com/science/article/pii/S1021949816300771 kostenfrei https://doaj.org/toc/1021-9498 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 24 2016 4 839-847
language	English
source	In Journal of Food and Drug Analysis 24(2016), 4, Seite 839-847 volume:24 year:2016 number:4 pages:839-847
sourceStr	In Journal of Food and Drug Analysis 24(2016), 4, Seite 839-847 volume:24 year:2016 number:4 pages:839-847
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Dendrobium huoshanense Dendrobium moniliforme Dendrobium officinale gas chromatography–mass spectrometry principal component analysis Nutrition. Foods and food supply Therapeutics. Pharmacology
isfreeaccess_bool	true
container_title	Journal of Food and Drug Analysis
authorswithroles_txt_mv	Nai-Dong Chen @@aut@@ Tao You @@aut@@ Jun Li @@aut@@ Li-Tao Bai @@aut@@ Jing-Wen Hao @@aut@@ Xiao-Yuan Xu @@aut@@
publishDateDaySort_date	2016-01-01T00:00:00Z
hierarchy_top_id	363771182
id	DOAJ053654862
language_de	englisch
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callnumber-first	T - Technology
author	Nai-Dong Chen
spellingShingle	Nai-Dong Chen misc TX341-641 misc RM1-950 misc Dendrobium huoshanense misc Dendrobium moniliforme misc Dendrobium officinale misc gas chromatography–mass spectrometry misc principal component analysis misc Nutrition. Foods and food supply misc Therapeutics. Pharmacology A comparative study of three tissue-cultured Dendrobium species and their wild correspondences by headspace gas chromatography–mass spectrometry combined with chemometric methods
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topic_title	TX341-641 RM1-950 A comparative study of three tissue-cultured Dendrobium species and their wild correspondences by headspace gas chromatography–mass spectrometry combined with chemometric methods Dendrobium huoshanense Dendrobium moniliforme Dendrobium officinale gas chromatography–mass spectrometry principal component analysis
topic	misc TX341-641 misc RM1-950 misc Dendrobium huoshanense misc Dendrobium moniliforme misc Dendrobium officinale misc gas chromatography–mass spectrometry misc principal component analysis misc Nutrition. Foods and food supply misc Therapeutics. Pharmacology
topic_unstemmed	misc TX341-641 misc RM1-950 misc Dendrobium huoshanense misc Dendrobium moniliforme misc Dendrobium officinale misc gas chromatography–mass spectrometry misc principal component analysis misc Nutrition. Foods and food supply misc Therapeutics. Pharmacology
topic_browse	misc TX341-641 misc RM1-950 misc Dendrobium huoshanense misc Dendrobium moniliforme misc Dendrobium officinale misc gas chromatography–mass spectrometry misc principal component analysis misc Nutrition. Foods and food supply misc Therapeutics. Pharmacology
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title	A comparative study of three tissue-cultured Dendrobium species and their wild correspondences by headspace gas chromatography–mass spectrometry combined with chemometric methods
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title_full	A comparative study of three tissue-cultured Dendrobium species and their wild correspondences by headspace gas chromatography–mass spectrometry combined with chemometric methods
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author_browse	Nai-Dong Chen Tao You Jun Li Li-Tao Bai Jing-Wen Hao Xiao-Yuan Xu
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title_sort	comparative study of three tissue-cultured dendrobium species and their wild correspondences by headspace gas chromatography–mass spectrometry combined with chemometric methods
callnumber	TX341-641
title_auth	A comparative study of three tissue-cultured Dendrobium species and their wild correspondences by headspace gas chromatography–mass spectrometry combined with chemometric methods
abstract	Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences. In this paper, a headspace gas chromatography–mass spectrometry method combined with chemometric methods was applied to analyze and evaluate the volatile compounds in tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme (Linn.) Sw. In total, 63 volatile compounds were separated, with 53 being identified from the three Dendrobium spp. samples. Different provenances of Dendrobiums had characteristic chemicals and showed remarkable quantity discrepancy of common compositions. The similarity evaluation disclosed that the accumulation of volatile compounds in Dendrobium samples might be affected by their provenance. Principal component analysis showed that the first three components explained 85.9% of data variance, demonstrating a good discrimination between samples. Gas chromatography–mass spectrometry techniques, combined with chemometrics, might be an effective strategy for identifying the species and their provenance, especially in the assessment of tissue-cultured Dendrobium quality for use in raw herbal medicines.
abstractGer	Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences. In this paper, a headspace gas chromatography–mass spectrometry method combined with chemometric methods was applied to analyze and evaluate the volatile compounds in tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme (Linn.) Sw. In total, 63 volatile compounds were separated, with 53 being identified from the three Dendrobium spp. samples. Different provenances of Dendrobiums had characteristic chemicals and showed remarkable quantity discrepancy of common compositions. The similarity evaluation disclosed that the accumulation of volatile compounds in Dendrobium samples might be affected by their provenance. Principal component analysis showed that the first three components explained 85.9% of data variance, demonstrating a good discrimination between samples. Gas chromatography–mass spectrometry techniques, combined with chemometrics, might be an effective strategy for identifying the species and their provenance, especially in the assessment of tissue-cultured Dendrobium quality for use in raw herbal medicines.
abstract_unstemmed	Plant tissue culture technique is widely used in the conservation and utilization of rare and endangered medicinal plants and it is crucial for tissue culture stocks to obtain the ability to produce similar bioactive components as their wild correspondences. In this paper, a headspace gas chromatography–mass spectrometry method combined with chemometric methods was applied to analyze and evaluate the volatile compounds in tissue-cultured and wild Dendrobium huoshanense Cheng and Tang, Dendrobium officinale Kimura et Migo and Dendrobium moniliforme (Linn.) Sw. In total, 63 volatile compounds were separated, with 53 being identified from the three Dendrobium spp. samples. Different provenances of Dendrobiums had characteristic chemicals and showed remarkable quantity discrepancy of common compositions. The similarity evaluation disclosed that the accumulation of volatile compounds in Dendrobium samples might be affected by their provenance. Principal component analysis showed that the first three components explained 85.9% of data variance, demonstrating a good discrimination between samples. Gas chromatography–mass spectrometry techniques, combined with chemometrics, might be an effective strategy for identifying the species and their provenance, especially in the assessment of tissue-cultured Dendrobium quality for use in raw herbal medicines.
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title_short	A comparative study of three tissue-cultured Dendrobium species and their wild correspondences by headspace gas chromatography–mass spectrometry combined with chemometric methods
url	https://doi.org/10.1016/j.jfda.2016.05.006 https://doaj.org/article/aa83e95e297741cbb422f4228a71a286 http://www.sciencedirect.com/science/article/pii/S1021949816300771 https://doaj.org/toc/1021-9498
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A comparative study of three tissue-cultured Dendrobium species and their wild correspondences by headspace gas chromatography–mass spectrometry combined with chemometric methods

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