Simultaneous miRNA and mRNA Transcriptome Profiling of Differentiating Equine Satellite Cells Treated with Gamma-Oryzanol and Exposed to Hydrogen Peroxide

Gamma-oryzanol (GO) is a popular supplement for performance horses, dogs, and humans. Previous studies indicated that GO supplementation decreases creatine kinase activity and lactate level after exercise and may affect oxidative stress in Thoroughbred horses. GO may change genes expression in equin...
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Autor*in:	Karolina A. Chodkowska [verfasserIn] Anna Ciecierska [verfasserIn] Kinga Majchrzak [verfasserIn] Piotr Ostaszewski [verfasserIn] Tomasz Sadkowski [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2018

Schlagwörter:	gamma-oryzanol equine satellite cells miRNA target genes hydrogen peroxide muscle injuries apoptosis oxidative stress

Übergeordnetes Werk:	In: Nutrients - MDPI AG, 2009, 10(2018), 12, p 1871
Übergeordnetes Werk:	volume:10 ; year:2018 ; number:12, p 1871

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/nu10121871

Katalog-ID:	DOAJ054543576

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source	In Nutrients 10(2018), 12, p 1871 volume:10 year:2018 number:12, p 1871
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callnumber-first	T - Technology
author	Karolina A. Chodkowska
spellingShingle	Karolina A. Chodkowska misc TX341-641 misc gamma-oryzanol misc equine satellite cells misc miRNA misc target genes misc hydrogen peroxide misc muscle injuries misc apoptosis misc oxidative stress misc Nutrition. Foods and food supply Simultaneous miRNA and mRNA Transcriptome Profiling of Differentiating Equine Satellite Cells Treated with Gamma-Oryzanol and Exposed to Hydrogen Peroxide
authorStr	Karolina A. Chodkowska
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topic_title	TX341-641 Simultaneous miRNA and mRNA Transcriptome Profiling of Differentiating Equine Satellite Cells Treated with Gamma-Oryzanol and Exposed to Hydrogen Peroxide gamma-oryzanol equine satellite cells miRNA target genes hydrogen peroxide muscle injuries apoptosis oxidative stress
topic	misc TX341-641 misc gamma-oryzanol misc equine satellite cells misc miRNA misc target genes misc hydrogen peroxide misc muscle injuries misc apoptosis misc oxidative stress misc Nutrition. Foods and food supply
topic_unstemmed	misc TX341-641 misc gamma-oryzanol misc equine satellite cells misc miRNA misc target genes misc hydrogen peroxide misc muscle injuries misc apoptosis misc oxidative stress misc Nutrition. Foods and food supply
topic_browse	misc TX341-641 misc gamma-oryzanol misc equine satellite cells misc miRNA misc target genes misc hydrogen peroxide misc muscle injuries misc apoptosis misc oxidative stress misc Nutrition. Foods and food supply
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title	Simultaneous miRNA and mRNA Transcriptome Profiling of Differentiating Equine Satellite Cells Treated with Gamma-Oryzanol and Exposed to Hydrogen Peroxide
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title_full	Simultaneous miRNA and mRNA Transcriptome Profiling of Differentiating Equine Satellite Cells Treated with Gamma-Oryzanol and Exposed to Hydrogen Peroxide
author_sort	Karolina A. Chodkowska
journal	Nutrients
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author_browse	Karolina A. Chodkowska Anna Ciecierska Kinga Majchrzak Piotr Ostaszewski Tomasz Sadkowski
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author-letter	Karolina A. Chodkowska
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title_sort	simultaneous mirna and mrna transcriptome profiling of differentiating equine satellite cells treated with gamma-oryzanol and exposed to hydrogen peroxide
callnumber	TX341-641
title_auth	Simultaneous miRNA and mRNA Transcriptome Profiling of Differentiating Equine Satellite Cells Treated with Gamma-Oryzanol and Exposed to Hydrogen Peroxide
abstract	Gamma-oryzanol (GO) is a popular supplement for performance horses, dogs, and humans. Previous studies indicated that GO supplementation decreases creatine kinase activity and lactate level after exercise and may affect oxidative stress in Thoroughbred horses. GO may change genes expression in equine satellite cells (ESC). The purpose of this study was to evaluate the effect of GO on miRNA, gene expression, oxidative stress, and cell damage and viability in differentiating ESC pretreated with hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<). ESCs were obtained from a young horse’s skeletal muscle. ESCs were pre-incubated with GO (24 h) and then exposed to H<sub<2</sub<O<sub<2</sub< for one hour. For the microRNA and gene expression assessment, the microarray technique was used. Identified miRNAs and genes were validated using real time-quantitative polymerase chain reaction. Several tests related to cell viability, cell damage, and oxidative stress were performed. The microarray analysis revealed differences in 17 miRNAs and 202 genes between GO-treated and control ESC. The tests related to apoptosis, cell viability, and oxidative stress showed that GO affects these processes to varying degrees. Our results suggest that GO can change miRNA and gene expression and may impact the processes involved in tissue repairing after an injury.
abstractGer	Gamma-oryzanol (GO) is a popular supplement for performance horses, dogs, and humans. Previous studies indicated that GO supplementation decreases creatine kinase activity and lactate level after exercise and may affect oxidative stress in Thoroughbred horses. GO may change genes expression in equine satellite cells (ESC). The purpose of this study was to evaluate the effect of GO on miRNA, gene expression, oxidative stress, and cell damage and viability in differentiating ESC pretreated with hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<). ESCs were obtained from a young horse’s skeletal muscle. ESCs were pre-incubated with GO (24 h) and then exposed to H<sub<2</sub<O<sub<2</sub< for one hour. For the microRNA and gene expression assessment, the microarray technique was used. Identified miRNAs and genes were validated using real time-quantitative polymerase chain reaction. Several tests related to cell viability, cell damage, and oxidative stress were performed. The microarray analysis revealed differences in 17 miRNAs and 202 genes between GO-treated and control ESC. The tests related to apoptosis, cell viability, and oxidative stress showed that GO affects these processes to varying degrees. Our results suggest that GO can change miRNA and gene expression and may impact the processes involved in tissue repairing after an injury.
abstract_unstemmed	Gamma-oryzanol (GO) is a popular supplement for performance horses, dogs, and humans. Previous studies indicated that GO supplementation decreases creatine kinase activity and lactate level after exercise and may affect oxidative stress in Thoroughbred horses. GO may change genes expression in equine satellite cells (ESC). The purpose of this study was to evaluate the effect of GO on miRNA, gene expression, oxidative stress, and cell damage and viability in differentiating ESC pretreated with hydrogen peroxide (H<sub<2</sub<O<sub<2</sub<). ESCs were obtained from a young horse’s skeletal muscle. ESCs were pre-incubated with GO (24 h) and then exposed to H<sub<2</sub<O<sub<2</sub< for one hour. For the microRNA and gene expression assessment, the microarray technique was used. Identified miRNAs and genes were validated using real time-quantitative polymerase chain reaction. Several tests related to cell viability, cell damage, and oxidative stress were performed. The microarray analysis revealed differences in 17 miRNAs and 202 genes between GO-treated and control ESC. The tests related to apoptosis, cell viability, and oxidative stress showed that GO affects these processes to varying degrees. Our results suggest that GO can change miRNA and gene expression and may impact the processes involved in tissue repairing after an injury.
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
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title_short	Simultaneous miRNA and mRNA Transcriptome Profiling of Differentiating Equine Satellite Cells Treated with Gamma-Oryzanol and Exposed to Hydrogen Peroxide
url	https://doi.org/10.3390/nu10121871 https://doaj.org/article/dc68c8ddef7f4309ae8c2fc1c9a21d17 https://www.mdpi.com/2072-6643/10/12/1871 https://doaj.org/toc/2072-6643
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author2	Anna Ciecierska Kinga Majchrzak Piotr Ostaszewski Tomasz Sadkowski
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up_date	2024-07-03T23:36:32.067Z
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Simultaneous miRNA and mRNA Transcriptome Profiling of Differentiating Equine Satellite Cells Treated with Gamma-Oryzanol and Exposed to Hydrogen Peroxide

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