Expression of the Melatonin-Associated Genes in Fibroblasts That Have Been Co-Exposed to Fluoride and a Moderate-Strength Static Magnetic Field

Fluoride can weaken the protective role of melatonin in reducing cellular damage. A static magnetic field is a physical factor that can counteract the negative effect of fluoride. Hence, the main objective of the study was to analyze the transcriptional activity of the genes that are associated with...
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Autor*in:	Celina Kruszniewska-Rajs [verfasserIn] Agnieszka Synowiec-Wojtarowicz [verfasserIn] Joanna Gola [verfasserIn] Magdalena Kimsa-Dudek [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	static magnetic field fluoride skin fibroblasts transcriptional activity of genes melatonin oxidative damage

Übergeordnetes Werk:	In: Applied Sciences - MDPI AG, 2012, 11(2021), 19, p 8810
Übergeordnetes Werk:	volume:11 ; year:2021 ; number:19, p 8810

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/app11198810

Katalog-ID:	DOAJ054554195

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520			\|a Fluoride can weaken the protective role of melatonin in reducing cellular damage. A static magnetic field is a physical factor that can counteract the negative effect of fluoride. Hence, the main objective of the study was to analyze the transcriptional activity of the genes that are associated with the activity of melatonin in human skin fibroblasts that have been co-exposed to fluoride and a moderate-strength static magnetic field. The expression of the melatonin-associated genes in human fibroblasts that had simultaneously been exposed to F<sup<−</sup< and a static magnetic field was determined using an oligonucleotide microarray and RT-qPCR techniques. The concentration of oxidative damage markers was also measured. In NaF and static magnetic field-treated cells, there was a tendency to compensate for the expression of the differentiating genes (<i<IL27RA</i<, <i<NR1D1</i<, <i<RRP7A</i<, <i<YIPF1</i<, <i<HIST1H2BD</i<) that had been modified by the presence of fluoride. It has been also shown that the oxidative damage marker concentration was statistically lower in the cells that had simultaneously been exposed to fluoride and a static magnetic field compared to the F-treated cells. In conclusion, the protective role of a moderate-strength static magnetic field on human dermal fibroblasts that had been exposed to fluoride was demonstrated, and its mechanism of action is associated with the melatonin-dependent pathways.
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callnumber-first	T - Technology
author	Celina Kruszniewska-Rajs
spellingShingle	Celina Kruszniewska-Rajs misc TA1-2040 misc QH301-705.5 misc QC1-999 misc QD1-999 misc static magnetic field misc fluoride misc skin fibroblasts misc transcriptional activity of genes misc melatonin misc oxidative damage misc Technology misc T misc Engineering (General). Civil engineering (General) misc Biology (General) misc Physics misc Chemistry Expression of the Melatonin-Associated Genes in Fibroblasts That Have Been Co-Exposed to Fluoride and a Moderate-Strength Static Magnetic Field
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topic_title	TA1-2040 QH301-705.5 QC1-999 QD1-999 Expression of the Melatonin-Associated Genes in Fibroblasts That Have Been Co-Exposed to Fluoride and a Moderate-Strength Static Magnetic Field static magnetic field fluoride skin fibroblasts transcriptional activity of genes melatonin oxidative damage
topic	misc TA1-2040 misc QH301-705.5 misc QC1-999 misc QD1-999 misc static magnetic field misc fluoride misc skin fibroblasts misc transcriptional activity of genes misc melatonin misc oxidative damage misc Technology misc T misc Engineering (General). Civil engineering (General) misc Biology (General) misc Physics misc Chemistry
topic_unstemmed	misc TA1-2040 misc QH301-705.5 misc QC1-999 misc QD1-999 misc static magnetic field misc fluoride misc skin fibroblasts misc transcriptional activity of genes misc melatonin misc oxidative damage misc Technology misc T misc Engineering (General). Civil engineering (General) misc Biology (General) misc Physics misc Chemistry
topic_browse	misc TA1-2040 misc QH301-705.5 misc QC1-999 misc QD1-999 misc static magnetic field misc fluoride misc skin fibroblasts misc transcriptional activity of genes misc melatonin misc oxidative damage misc Technology misc T misc Engineering (General). Civil engineering (General) misc Biology (General) misc Physics misc Chemistry
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title	Expression of the Melatonin-Associated Genes in Fibroblasts That Have Been Co-Exposed to Fluoride and a Moderate-Strength Static Magnetic Field
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title_full	Expression of the Melatonin-Associated Genes in Fibroblasts That Have Been Co-Exposed to Fluoride and a Moderate-Strength Static Magnetic Field
author_sort	Celina Kruszniewska-Rajs
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author_browse	Celina Kruszniewska-Rajs Agnieszka Synowiec-Wojtarowicz Joanna Gola Magdalena Kimsa-Dudek
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title_sort	expression of the melatonin-associated genes in fibroblasts that have been co-exposed to fluoride and a moderate-strength static magnetic field
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title_auth	Expression of the Melatonin-Associated Genes in Fibroblasts That Have Been Co-Exposed to Fluoride and a Moderate-Strength Static Magnetic Field
abstract	Fluoride can weaken the protective role of melatonin in reducing cellular damage. A static magnetic field is a physical factor that can counteract the negative effect of fluoride. Hence, the main objective of the study was to analyze the transcriptional activity of the genes that are associated with the activity of melatonin in human skin fibroblasts that have been co-exposed to fluoride and a moderate-strength static magnetic field. The expression of the melatonin-associated genes in human fibroblasts that had simultaneously been exposed to F<sup<−</sup< and a static magnetic field was determined using an oligonucleotide microarray and RT-qPCR techniques. The concentration of oxidative damage markers was also measured. In NaF and static magnetic field-treated cells, there was a tendency to compensate for the expression of the differentiating genes (<i<IL27RA</i<, <i<NR1D1</i<, <i<RRP7A</i<, <i<YIPF1</i<, <i<HIST1H2BD</i<) that had been modified by the presence of fluoride. It has been also shown that the oxidative damage marker concentration was statistically lower in the cells that had simultaneously been exposed to fluoride and a static magnetic field compared to the F-treated cells. In conclusion, the protective role of a moderate-strength static magnetic field on human dermal fibroblasts that had been exposed to fluoride was demonstrated, and its mechanism of action is associated with the melatonin-dependent pathways.
abstractGer	Fluoride can weaken the protective role of melatonin in reducing cellular damage. A static magnetic field is a physical factor that can counteract the negative effect of fluoride. Hence, the main objective of the study was to analyze the transcriptional activity of the genes that are associated with the activity of melatonin in human skin fibroblasts that have been co-exposed to fluoride and a moderate-strength static magnetic field. The expression of the melatonin-associated genes in human fibroblasts that had simultaneously been exposed to F<sup<−</sup< and a static magnetic field was determined using an oligonucleotide microarray and RT-qPCR techniques. The concentration of oxidative damage markers was also measured. In NaF and static magnetic field-treated cells, there was a tendency to compensate for the expression of the differentiating genes (<i<IL27RA</i<, <i<NR1D1</i<, <i<RRP7A</i<, <i<YIPF1</i<, <i<HIST1H2BD</i<) that had been modified by the presence of fluoride. It has been also shown that the oxidative damage marker concentration was statistically lower in the cells that had simultaneously been exposed to fluoride and a static magnetic field compared to the F-treated cells. In conclusion, the protective role of a moderate-strength static magnetic field on human dermal fibroblasts that had been exposed to fluoride was demonstrated, and its mechanism of action is associated with the melatonin-dependent pathways.
abstract_unstemmed	Fluoride can weaken the protective role of melatonin in reducing cellular damage. A static magnetic field is a physical factor that can counteract the negative effect of fluoride. Hence, the main objective of the study was to analyze the transcriptional activity of the genes that are associated with the activity of melatonin in human skin fibroblasts that have been co-exposed to fluoride and a moderate-strength static magnetic field. The expression of the melatonin-associated genes in human fibroblasts that had simultaneously been exposed to F<sup<−</sup< and a static magnetic field was determined using an oligonucleotide microarray and RT-qPCR techniques. The concentration of oxidative damage markers was also measured. In NaF and static magnetic field-treated cells, there was a tendency to compensate for the expression of the differentiating genes (<i<IL27RA</i<, <i<NR1D1</i<, <i<RRP7A</i<, <i<YIPF1</i<, <i<HIST1H2BD</i<) that had been modified by the presence of fluoride. It has been also shown that the oxidative damage marker concentration was statistically lower in the cells that had simultaneously been exposed to fluoride and a static magnetic field compared to the F-treated cells. In conclusion, the protective role of a moderate-strength static magnetic field on human dermal fibroblasts that had been exposed to fluoride was demonstrated, and its mechanism of action is associated with the melatonin-dependent pathways.
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container_issue	19, p 8810
title_short	Expression of the Melatonin-Associated Genes in Fibroblasts That Have Been Co-Exposed to Fluoride and a Moderate-Strength Static Magnetic Field
url	https://doi.org/10.3390/app11198810 https://doaj.org/article/f3f09fa9bf2f443090a877c779966fe1 https://www.mdpi.com/2076-3417/11/19/8810 https://doaj.org/toc/2076-3417
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A static magnetic field is a physical factor that can counteract the negative effect of fluoride. Hence, the main objective of the study was to analyze the transcriptional activity of the genes that are associated with the activity of melatonin in human skin fibroblasts that have been co-exposed to fluoride and a moderate-strength static magnetic field. The expression of the melatonin-associated genes in human fibroblasts that had simultaneously been exposed to F<sup<−</sup< and a static magnetic field was determined using an oligonucleotide microarray and RT-qPCR techniques. The concentration of oxidative damage markers was also measured. In NaF and static magnetic field-treated cells, there was a tendency to compensate for the expression of the differentiating genes (<i<IL27RA</i<, <i<NR1D1</i<, <i<RRP7A</i<, <i<YIPF1</i<, <i<HIST1H2BD</i<) that had been modified by the presence of fluoride. It has been also shown that the oxidative damage marker concentration was statistically lower in the cells that had simultaneously been exposed to fluoride and a static magnetic field compared to the F-treated cells. In conclusion, the protective role of a moderate-strength static magnetic field on human dermal fibroblasts that had been exposed to fluoride was demonstrated, and its mechanism of action is associated with the melatonin-dependent pathways.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">static magnetic field</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">fluoride</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">skin fibroblasts</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">transcriptional activity of genes</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">melatonin</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">oxidative damage</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Technology</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">T</subfield></datafield><datafield tag="653" ind1=" " ind2="0"><subfield code="a">Engineering (General). 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Expression of the Melatonin-Associated Genes in Fibroblasts That Have Been Co-Exposed to Fluoride and a Moderate-Strength Static Magnetic Field

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