Determination of biomarker IL-6 in exhaled breath condensate using exhaled breath condensate collector
Background and Objective: The collection of exhaled breath condensate (EBC) is a noninvasive, safe and simple technique to obtain direct samples from lung and use for estimation of the effects of air pollution on human subjects. EBC contains several compounds including simple ions, hydrogen peroxide...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
M Seifi [verfasserIn] N Rastkari [verfasserIn] MS Hassanvand [verfasserIn] H Arfaeinia [verfasserIn] M Younesian [verfasserIn] |
|---|
| Format: |
E-Artikel |
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| Sprache: |
Persisch |
| Erschienen: |
2017 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
In: سلامت و محیط - Tehran University of Medical Sciences, 2021, 10(2017), 1, Seite 15-24 |
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| Übergeordnetes Werk: |
volume:10 ; year:2017 ; number:1 ; pages:15-24 |
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DOAJ054875315 |
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| 520 | |a Background and Objective: The collection of exhaled breath condensate (EBC) is a noninvasive, safe and simple technique to obtain direct samples from lung and use for estimation of the effects of air pollution on human subjects. EBC contains several compounds including simple ions, hydrogen peroxide, proteins, cytokines, deoxyribonucleic acid (DNA), creatine, Electrolytes, trace elements and toxic metals that are diluted by water vapor. The objective of this study was to determine biomarker IL-6 in exhaled breath condensate using an exhaled breath condensate collector. Materials and Methods: The collector was designed and built and was used for collection and condensation of exhaled breath. EBC was taken from 36 young participants. Each person was asked to breathe for 10-15 min and the condensate was kept in a vial. The samples of exhaled breath were cooled down to -20 °C using the collector. The exhaled breath was converted to the solid/liquid mixture and then, the biomarker IL-6 was measured with ELISA (Enzyme -linked immunosorbent assay) method using HS600B R&D Kit. Results: The mean concentration of IL-6 was 1.08 pg/mL in collected samples. The standard deviation of IL-6 concentration was 0.47. Moreover, the average volume of collected EBC was 2.3 mL, ranged between 0.3 and 3.5 mL. Conclusion: The results of this study showed that the volume of the exhaled breath condensate was comparable with similar commercial devices. The average concentration of interlukin-6 was similar to the concentrations reported by other studies. Based on the findings, this equipment can be used by researchers as a suitable device for measurement of IL-6 in exhaled breath condensate. | ||
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(DE-627)DOAJ054875315 (DE-599)DOAJfa53d473afa54a9c8f994adc07ce515a DE-627 ger DE-627 rakwb per GE1-350 M Seifi verfasserin aut Determination of biomarker IL-6 in exhaled breath condensate using exhaled breath condensate collector 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background and Objective: The collection of exhaled breath condensate (EBC) is a noninvasive, safe and simple technique to obtain direct samples from lung and use for estimation of the effects of air pollution on human subjects. EBC contains several compounds including simple ions, hydrogen peroxide, proteins, cytokines, deoxyribonucleic acid (DNA), creatine, Electrolytes, trace elements and toxic metals that are diluted by water vapor. The objective of this study was to determine biomarker IL-6 in exhaled breath condensate using an exhaled breath condensate collector. Materials and Methods: The collector was designed and built and was used for collection and condensation of exhaled breath. EBC was taken from 36 young participants. Each person was asked to breathe for 10-15 min and the condensate was kept in a vial. The samples of exhaled breath were cooled down to -20 °C using the collector. The exhaled breath was converted to the solid/liquid mixture and then, the biomarker IL-6 was measured with ELISA (Enzyme -linked immunosorbent assay) method using HS600B R&D Kit. Results: The mean concentration of IL-6 was 1.08 pg/mL in collected samples. The standard deviation of IL-6 concentration was 0.47. Moreover, the average volume of collected EBC was 2.3 mL, ranged between 0.3 and 3.5 mL. Conclusion: The results of this study showed that the volume of the exhaled breath condensate was comparable with similar commercial devices. The average concentration of interlukin-6 was similar to the concentrations reported by other studies. Based on the findings, this equipment can be used by researchers as a suitable device for measurement of IL-6 in exhaled breath condensate. exhaled breath condensate biomarker condenser Environmental sciences N Rastkari verfasserin aut MS Hassanvand verfasserin aut H Arfaeinia verfasserin aut M Younesian verfasserin aut In سلامت و محیط Tehran University of Medical Sciences, 2021 10(2017), 1, Seite 15-24 (DE-627)DOAJ078612497 20083718 nnns volume:10 year:2017 number:1 pages:15-24 https://doaj.org/article/fa53d473afa54a9c8f994adc07ce515a kostenfrei http://ijhe.tums.ac.ir/article-1-5185-en.html kostenfrei https://doaj.org/toc/2008-2029 Journal toc kostenfrei https://doaj.org/toc/2008-3718 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 10 2017 1 15-24 |
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(DE-627)DOAJ054875315 (DE-599)DOAJfa53d473afa54a9c8f994adc07ce515a DE-627 ger DE-627 rakwb per GE1-350 M Seifi verfasserin aut Determination of biomarker IL-6 in exhaled breath condensate using exhaled breath condensate collector 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background and Objective: The collection of exhaled breath condensate (EBC) is a noninvasive, safe and simple technique to obtain direct samples from lung and use for estimation of the effects of air pollution on human subjects. EBC contains several compounds including simple ions, hydrogen peroxide, proteins, cytokines, deoxyribonucleic acid (DNA), creatine, Electrolytes, trace elements and toxic metals that are diluted by water vapor. The objective of this study was to determine biomarker IL-6 in exhaled breath condensate using an exhaled breath condensate collector. Materials and Methods: The collector was designed and built and was used for collection and condensation of exhaled breath. EBC was taken from 36 young participants. Each person was asked to breathe for 10-15 min and the condensate was kept in a vial. The samples of exhaled breath were cooled down to -20 °C using the collector. The exhaled breath was converted to the solid/liquid mixture and then, the biomarker IL-6 was measured with ELISA (Enzyme -linked immunosorbent assay) method using HS600B R&D Kit. Results: The mean concentration of IL-6 was 1.08 pg/mL in collected samples. The standard deviation of IL-6 concentration was 0.47. Moreover, the average volume of collected EBC was 2.3 mL, ranged between 0.3 and 3.5 mL. Conclusion: The results of this study showed that the volume of the exhaled breath condensate was comparable with similar commercial devices. The average concentration of interlukin-6 was similar to the concentrations reported by other studies. Based on the findings, this equipment can be used by researchers as a suitable device for measurement of IL-6 in exhaled breath condensate. exhaled breath condensate biomarker condenser Environmental sciences N Rastkari verfasserin aut MS Hassanvand verfasserin aut H Arfaeinia verfasserin aut M Younesian verfasserin aut In سلامت و محیط Tehran University of Medical Sciences, 2021 10(2017), 1, Seite 15-24 (DE-627)DOAJ078612497 20083718 nnns volume:10 year:2017 number:1 pages:15-24 https://doaj.org/article/fa53d473afa54a9c8f994adc07ce515a kostenfrei http://ijhe.tums.ac.ir/article-1-5185-en.html kostenfrei https://doaj.org/toc/2008-2029 Journal toc kostenfrei https://doaj.org/toc/2008-3718 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 10 2017 1 15-24 |
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(DE-627)DOAJ054875315 (DE-599)DOAJfa53d473afa54a9c8f994adc07ce515a DE-627 ger DE-627 rakwb per GE1-350 M Seifi verfasserin aut Determination of biomarker IL-6 in exhaled breath condensate using exhaled breath condensate collector 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background and Objective: The collection of exhaled breath condensate (EBC) is a noninvasive, safe and simple technique to obtain direct samples from lung and use for estimation of the effects of air pollution on human subjects. EBC contains several compounds including simple ions, hydrogen peroxide, proteins, cytokines, deoxyribonucleic acid (DNA), creatine, Electrolytes, trace elements and toxic metals that are diluted by water vapor. The objective of this study was to determine biomarker IL-6 in exhaled breath condensate using an exhaled breath condensate collector. Materials and Methods: The collector was designed and built and was used for collection and condensation of exhaled breath. EBC was taken from 36 young participants. Each person was asked to breathe for 10-15 min and the condensate was kept in a vial. The samples of exhaled breath were cooled down to -20 °C using the collector. The exhaled breath was converted to the solid/liquid mixture and then, the biomarker IL-6 was measured with ELISA (Enzyme -linked immunosorbent assay) method using HS600B R&D Kit. Results: The mean concentration of IL-6 was 1.08 pg/mL in collected samples. The standard deviation of IL-6 concentration was 0.47. Moreover, the average volume of collected EBC was 2.3 mL, ranged between 0.3 and 3.5 mL. Conclusion: The results of this study showed that the volume of the exhaled breath condensate was comparable with similar commercial devices. The average concentration of interlukin-6 was similar to the concentrations reported by other studies. Based on the findings, this equipment can be used by researchers as a suitable device for measurement of IL-6 in exhaled breath condensate. exhaled breath condensate biomarker condenser Environmental sciences N Rastkari verfasserin aut MS Hassanvand verfasserin aut H Arfaeinia verfasserin aut M Younesian verfasserin aut In سلامت و محیط Tehran University of Medical Sciences, 2021 10(2017), 1, Seite 15-24 (DE-627)DOAJ078612497 20083718 nnns volume:10 year:2017 number:1 pages:15-24 https://doaj.org/article/fa53d473afa54a9c8f994adc07ce515a kostenfrei http://ijhe.tums.ac.ir/article-1-5185-en.html kostenfrei https://doaj.org/toc/2008-2029 Journal toc kostenfrei https://doaj.org/toc/2008-3718 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 10 2017 1 15-24 |
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(DE-627)DOAJ054875315 (DE-599)DOAJfa53d473afa54a9c8f994adc07ce515a DE-627 ger DE-627 rakwb per GE1-350 M Seifi verfasserin aut Determination of biomarker IL-6 in exhaled breath condensate using exhaled breath condensate collector 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background and Objective: The collection of exhaled breath condensate (EBC) is a noninvasive, safe and simple technique to obtain direct samples from lung and use for estimation of the effects of air pollution on human subjects. EBC contains several compounds including simple ions, hydrogen peroxide, proteins, cytokines, deoxyribonucleic acid (DNA), creatine, Electrolytes, trace elements and toxic metals that are diluted by water vapor. The objective of this study was to determine biomarker IL-6 in exhaled breath condensate using an exhaled breath condensate collector. Materials and Methods: The collector was designed and built and was used for collection and condensation of exhaled breath. EBC was taken from 36 young participants. Each person was asked to breathe for 10-15 min and the condensate was kept in a vial. The samples of exhaled breath were cooled down to -20 °C using the collector. The exhaled breath was converted to the solid/liquid mixture and then, the biomarker IL-6 was measured with ELISA (Enzyme -linked immunosorbent assay) method using HS600B R&D Kit. Results: The mean concentration of IL-6 was 1.08 pg/mL in collected samples. The standard deviation of IL-6 concentration was 0.47. Moreover, the average volume of collected EBC was 2.3 mL, ranged between 0.3 and 3.5 mL. Conclusion: The results of this study showed that the volume of the exhaled breath condensate was comparable with similar commercial devices. The average concentration of interlukin-6 was similar to the concentrations reported by other studies. Based on the findings, this equipment can be used by researchers as a suitable device for measurement of IL-6 in exhaled breath condensate. exhaled breath condensate biomarker condenser Environmental sciences N Rastkari verfasserin aut MS Hassanvand verfasserin aut H Arfaeinia verfasserin aut M Younesian verfasserin aut In سلامت و محیط Tehran University of Medical Sciences, 2021 10(2017), 1, Seite 15-24 (DE-627)DOAJ078612497 20083718 nnns volume:10 year:2017 number:1 pages:15-24 https://doaj.org/article/fa53d473afa54a9c8f994adc07ce515a kostenfrei http://ijhe.tums.ac.ir/article-1-5185-en.html kostenfrei https://doaj.org/toc/2008-2029 Journal toc kostenfrei https://doaj.org/toc/2008-3718 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 10 2017 1 15-24 |
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(DE-627)DOAJ054875315 (DE-599)DOAJfa53d473afa54a9c8f994adc07ce515a DE-627 ger DE-627 rakwb per GE1-350 M Seifi verfasserin aut Determination of biomarker IL-6 in exhaled breath condensate using exhaled breath condensate collector 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background and Objective: The collection of exhaled breath condensate (EBC) is a noninvasive, safe and simple technique to obtain direct samples from lung and use for estimation of the effects of air pollution on human subjects. EBC contains several compounds including simple ions, hydrogen peroxide, proteins, cytokines, deoxyribonucleic acid (DNA), creatine, Electrolytes, trace elements and toxic metals that are diluted by water vapor. The objective of this study was to determine biomarker IL-6 in exhaled breath condensate using an exhaled breath condensate collector. Materials and Methods: The collector was designed and built and was used for collection and condensation of exhaled breath. EBC was taken from 36 young participants. Each person was asked to breathe for 10-15 min and the condensate was kept in a vial. The samples of exhaled breath were cooled down to -20 °C using the collector. The exhaled breath was converted to the solid/liquid mixture and then, the biomarker IL-6 was measured with ELISA (Enzyme -linked immunosorbent assay) method using HS600B R&D Kit. Results: The mean concentration of IL-6 was 1.08 pg/mL in collected samples. The standard deviation of IL-6 concentration was 0.47. Moreover, the average volume of collected EBC was 2.3 mL, ranged between 0.3 and 3.5 mL. Conclusion: The results of this study showed that the volume of the exhaled breath condensate was comparable with similar commercial devices. The average concentration of interlukin-6 was similar to the concentrations reported by other studies. Based on the findings, this equipment can be used by researchers as a suitable device for measurement of IL-6 in exhaled breath condensate. exhaled breath condensate biomarker condenser Environmental sciences N Rastkari verfasserin aut MS Hassanvand verfasserin aut H Arfaeinia verfasserin aut M Younesian verfasserin aut In سلامت و محیط Tehran University of Medical Sciences, 2021 10(2017), 1, Seite 15-24 (DE-627)DOAJ078612497 20083718 nnns volume:10 year:2017 number:1 pages:15-24 https://doaj.org/article/fa53d473afa54a9c8f994adc07ce515a kostenfrei http://ijhe.tums.ac.ir/article-1-5185-en.html kostenfrei https://doaj.org/toc/2008-2029 Journal toc kostenfrei https://doaj.org/toc/2008-3718 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ AR 10 2017 1 15-24 |
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M Seifi N Rastkari MS Hassanvand H Arfaeinia M Younesian |
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determination of biomarker il-6 in exhaled breath condensate using exhaled breath condensate collector |
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GE1-350 |
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Determination of biomarker IL-6 in exhaled breath condensate using exhaled breath condensate collector |
| abstract |
Background and Objective: The collection of exhaled breath condensate (EBC) is a noninvasive, safe and simple technique to obtain direct samples from lung and use for estimation of the effects of air pollution on human subjects. EBC contains several compounds including simple ions, hydrogen peroxide, proteins, cytokines, deoxyribonucleic acid (DNA), creatine, Electrolytes, trace elements and toxic metals that are diluted by water vapor. The objective of this study was to determine biomarker IL-6 in exhaled breath condensate using an exhaled breath condensate collector. Materials and Methods: The collector was designed and built and was used for collection and condensation of exhaled breath. EBC was taken from 36 young participants. Each person was asked to breathe for 10-15 min and the condensate was kept in a vial. The samples of exhaled breath were cooled down to -20 °C using the collector. The exhaled breath was converted to the solid/liquid mixture and then, the biomarker IL-6 was measured with ELISA (Enzyme -linked immunosorbent assay) method using HS600B R&D Kit. Results: The mean concentration of IL-6 was 1.08 pg/mL in collected samples. The standard deviation of IL-6 concentration was 0.47. Moreover, the average volume of collected EBC was 2.3 mL, ranged between 0.3 and 3.5 mL. Conclusion: The results of this study showed that the volume of the exhaled breath condensate was comparable with similar commercial devices. The average concentration of interlukin-6 was similar to the concentrations reported by other studies. Based on the findings, this equipment can be used by researchers as a suitable device for measurement of IL-6 in exhaled breath condensate. |
| abstractGer |
Background and Objective: The collection of exhaled breath condensate (EBC) is a noninvasive, safe and simple technique to obtain direct samples from lung and use for estimation of the effects of air pollution on human subjects. EBC contains several compounds including simple ions, hydrogen peroxide, proteins, cytokines, deoxyribonucleic acid (DNA), creatine, Electrolytes, trace elements and toxic metals that are diluted by water vapor. The objective of this study was to determine biomarker IL-6 in exhaled breath condensate using an exhaled breath condensate collector. Materials and Methods: The collector was designed and built and was used for collection and condensation of exhaled breath. EBC was taken from 36 young participants. Each person was asked to breathe for 10-15 min and the condensate was kept in a vial. The samples of exhaled breath were cooled down to -20 °C using the collector. The exhaled breath was converted to the solid/liquid mixture and then, the biomarker IL-6 was measured with ELISA (Enzyme -linked immunosorbent assay) method using HS600B R&D Kit. Results: The mean concentration of IL-6 was 1.08 pg/mL in collected samples. The standard deviation of IL-6 concentration was 0.47. Moreover, the average volume of collected EBC was 2.3 mL, ranged between 0.3 and 3.5 mL. Conclusion: The results of this study showed that the volume of the exhaled breath condensate was comparable with similar commercial devices. The average concentration of interlukin-6 was similar to the concentrations reported by other studies. Based on the findings, this equipment can be used by researchers as a suitable device for measurement of IL-6 in exhaled breath condensate. |
| abstract_unstemmed |
Background and Objective: The collection of exhaled breath condensate (EBC) is a noninvasive, safe and simple technique to obtain direct samples from lung and use for estimation of the effects of air pollution on human subjects. EBC contains several compounds including simple ions, hydrogen peroxide, proteins, cytokines, deoxyribonucleic acid (DNA), creatine, Electrolytes, trace elements and toxic metals that are diluted by water vapor. The objective of this study was to determine biomarker IL-6 in exhaled breath condensate using an exhaled breath condensate collector. Materials and Methods: The collector was designed and built and was used for collection and condensation of exhaled breath. EBC was taken from 36 young participants. Each person was asked to breathe for 10-15 min and the condensate was kept in a vial. The samples of exhaled breath were cooled down to -20 °C using the collector. The exhaled breath was converted to the solid/liquid mixture and then, the biomarker IL-6 was measured with ELISA (Enzyme -linked immunosorbent assay) method using HS600B R&D Kit. Results: The mean concentration of IL-6 was 1.08 pg/mL in collected samples. The standard deviation of IL-6 concentration was 0.47. Moreover, the average volume of collected EBC was 2.3 mL, ranged between 0.3 and 3.5 mL. Conclusion: The results of this study showed that the volume of the exhaled breath condensate was comparable with similar commercial devices. The average concentration of interlukin-6 was similar to the concentrations reported by other studies. Based on the findings, this equipment can be used by researchers as a suitable device for measurement of IL-6 in exhaled breath condensate. |
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Determination of biomarker IL-6 in exhaled breath condensate using exhaled breath condensate collector |
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https://doaj.org/article/fa53d473afa54a9c8f994adc07ce515a http://ijhe.tums.ac.ir/article-1-5185-en.html https://doaj.org/toc/2008-2029 https://doaj.org/toc/2008-3718 |
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