Massive Integration of Planktonic Cells within a Developing Biofilm

During biofilm growth, the coexistence of planktonic and sessile cells can lead to dynamic exchanges between the two populations. We have monitored the fate of these populations in glass tube assays, where the <i<Bacillus thuringiensis</i< 407 strain produces a floating pellicle. Time-la...
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Gespeichert in:

Autor*in:	Nay El-Khoury [verfasserIn] Imene Bennaceur [verfasserIn] Emilie Verplaetse [verfasserIn] Stéphane Aymerich [verfasserIn] Didier Lereclus [verfasserIn] Mireille Kallassy [verfasserIn] Michel Gohar [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	biofilm recruitment heterogeneity

Übergeordnetes Werk:	In: Microorganisms - MDPI AG, 2013, 9(2021), 2, p 298
Übergeordnetes Werk:	volume:9 ; year:2021 ; number:2, p 298

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/microorganisms9020298

Katalog-ID:	DOAJ05499599X

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author	Nay El-Khoury
spellingShingle	Nay El-Khoury misc QH301-705.5 misc biofilm misc <i<Bacillus cereus</i< misc <i<Bacillus thuringiensis</i< misc recruitment misc heterogeneity misc Biology (General) Massive Integration of Planktonic Cells within a Developing Biofilm
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topic_title	QH301-705.5 Massive Integration of Planktonic Cells within a Developing Biofilm biofilm <i<Bacillus cereus</i< <i<Bacillus thuringiensis</i< recruitment heterogeneity
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title	Massive Integration of Planktonic Cells within a Developing Biofilm
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title_full	Massive Integration of Planktonic Cells within a Developing Biofilm
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title_sort	massive integration of planktonic cells within a developing biofilm
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title_auth	Massive Integration of Planktonic Cells within a Developing Biofilm
abstract	During biofilm growth, the coexistence of planktonic and sessile cells can lead to dynamic exchanges between the two populations. We have monitored the fate of these populations in glass tube assays, where the <i<Bacillus thuringiensis</i< 407 strain produces a floating pellicle. Time-lapse spectrophotometric measurement methods revealed that the planktonic population grew until the pellicle started to be produced. Thereafter, the planktonic population decreased rapidly down to a value close to zero while the biofilm was in continuous growth, showing no dispersal until 120 h of culture. We found that this decrease was induced by the presence of the pellicle, but did not occur when oxygen availability was limited, suggesting that it was independent of cell death or cell sedimentation and that the entire planktonic population has integrated the biofilm. To follow the distribution of recruited planktonic cells within the pellicle, we tagged planktonic cells with GFP and sessile cells with mCherry. Fluorescence binocular microscopy observations revealed that planktonic cells, injected through a 24-h-aged pellicle, were found only in specific areas of the biofilm, where the density of sessile cells was low, showing that spatial heterogeneity can occur between recruited cells and sessile cells in a monospecies biofilm.
abstractGer	During biofilm growth, the coexistence of planktonic and sessile cells can lead to dynamic exchanges between the two populations. We have monitored the fate of these populations in glass tube assays, where the <i<Bacillus thuringiensis</i< 407 strain produces a floating pellicle. Time-lapse spectrophotometric measurement methods revealed that the planktonic population grew until the pellicle started to be produced. Thereafter, the planktonic population decreased rapidly down to a value close to zero while the biofilm was in continuous growth, showing no dispersal until 120 h of culture. We found that this decrease was induced by the presence of the pellicle, but did not occur when oxygen availability was limited, suggesting that it was independent of cell death or cell sedimentation and that the entire planktonic population has integrated the biofilm. To follow the distribution of recruited planktonic cells within the pellicle, we tagged planktonic cells with GFP and sessile cells with mCherry. Fluorescence binocular microscopy observations revealed that planktonic cells, injected through a 24-h-aged pellicle, were found only in specific areas of the biofilm, where the density of sessile cells was low, showing that spatial heterogeneity can occur between recruited cells and sessile cells in a monospecies biofilm.
abstract_unstemmed	During biofilm growth, the coexistence of planktonic and sessile cells can lead to dynamic exchanges between the two populations. We have monitored the fate of these populations in glass tube assays, where the <i<Bacillus thuringiensis</i< 407 strain produces a floating pellicle. Time-lapse spectrophotometric measurement methods revealed that the planktonic population grew until the pellicle started to be produced. Thereafter, the planktonic population decreased rapidly down to a value close to zero while the biofilm was in continuous growth, showing no dispersal until 120 h of culture. We found that this decrease was induced by the presence of the pellicle, but did not occur when oxygen availability was limited, suggesting that it was independent of cell death or cell sedimentation and that the entire planktonic population has integrated the biofilm. To follow the distribution of recruited planktonic cells within the pellicle, we tagged planktonic cells with GFP and sessile cells with mCherry. Fluorescence binocular microscopy observations revealed that planktonic cells, injected through a 24-h-aged pellicle, were found only in specific areas of the biofilm, where the density of sessile cells was low, showing that spatial heterogeneity can occur between recruited cells and sessile cells in a monospecies biofilm.
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title_short	Massive Integration of Planktonic Cells within a Developing Biofilm
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