DHA induces ER stress and growth arrest in human colon cancer cells: associations with cholesterol and calcium homeostasis *s⃞
Polyunsaturated fatty acids (PUFAs) are normal constituents of the diet, but have properties different from other fatty acids (e.g., through generation of signaling molecules). N-3 PUFAs reduce cancer cell growth, but no unified mechanism has been identified. We show that docosahexaenoic acid (DHA;...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Caroline Hild Jakobsen [verfasserIn] Gro Leite Størvold [verfasserIn] Hilde Bremseth [verfasserIn] Turid Follestad [verfasserIn] Kristin Sand [verfasserIn] Merete Mack [verfasserIn] Karina Standahl Olsen [verfasserIn] Anne Gøril Lundemo [verfasserIn] Jens Gustav Iversen [verfasserIn] Hans Einar Krokan [verfasserIn] Svanhild Arentz Schønberg [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2008 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
In: Journal of Lipid Research - Elsevier, 2021, 49(2008), 10, Seite 2089-2100 |
|---|---|
| Übergeordnetes Werk: |
volume:49 ; year:2008 ; number:10 ; pages:2089-2100 |
| Links: |
|---|
| DOI / URN: |
10.1194/jlr.M700389-JLR200 |
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| Katalog-ID: |
DOAJ055454739 |
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| 520 | |a Polyunsaturated fatty acids (PUFAs) are normal constituents of the diet, but have properties different from other fatty acids (e.g., through generation of signaling molecules). N-3 PUFAs reduce cancer cell growth, but no unified mechanism has been identified. We show that docosahexaenoic acid (DHA; 22:6 n-3) causes extensive changes in gene expression patterns at mRNA level in the colon cancer cell line SW620. Early changes include unfolded protein response (UPR) and increased levels of phosphorylated eIF2α as verified at protein level. The latter is considered a hallmark of endoplasmic reticulum (ER) stress and is abundantly present already after 3 h. It may coordinate many of the downstream changes observed, including signaling pathways for cell cycle arrest/apoptosis, calcium homeostasis, cholesterol metabolism, ubiquitination, and proteasomal degradation. Also, eicosapentaenoic acid (EPA), but not oleic acid (OA), induced key mediators of ER stress and UPR at protein level. Accumulation of esterified cholesterol was not compensated for by increased total levels of cholesterol, and mRNAs for cholesterol biosynthesis as well as de novo synthesis of cholesterol were reduced. These results suggest that cytotoxic effects of DHA are associated with signaling pathways involving lipid metabolism and ER stress. | ||
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10.1194/jlr.M700389-JLR200 doi (DE-627)DOAJ055454739 (DE-599)DOAJfb81cf3aee5e4ebbad547dc6be7d20e4 DE-627 ger DE-627 rakwb eng QD415-436 Caroline Hild Jakobsen verfasserin aut DHA induces ER stress and growth arrest in human colon cancer cells: associations with cholesterol and calcium homeostasis *s⃞ 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Polyunsaturated fatty acids (PUFAs) are normal constituents of the diet, but have properties different from other fatty acids (e.g., through generation of signaling molecules). N-3 PUFAs reduce cancer cell growth, but no unified mechanism has been identified. We show that docosahexaenoic acid (DHA; 22:6 n-3) causes extensive changes in gene expression patterns at mRNA level in the colon cancer cell line SW620. Early changes include unfolded protein response (UPR) and increased levels of phosphorylated eIF2α as verified at protein level. The latter is considered a hallmark of endoplasmic reticulum (ER) stress and is abundantly present already after 3 h. It may coordinate many of the downstream changes observed, including signaling pathways for cell cycle arrest/apoptosis, calcium homeostasis, cholesterol metabolism, ubiquitination, and proteasomal degradation. Also, eicosapentaenoic acid (EPA), but not oleic acid (OA), induced key mediators of ER stress and UPR at protein level. Accumulation of esterified cholesterol was not compensated for by increased total levels of cholesterol, and mRNAs for cholesterol biosynthesis as well as de novo synthesis of cholesterol were reduced. These results suggest that cytotoxic effects of DHA are associated with signaling pathways involving lipid metabolism and ER stress. gene expression phosphorylated eIF2α antioxidant response heat shock response cytosolic free Ca2+ cell cycle Biochemistry Gro Leite Størvold verfasserin aut Hilde Bremseth verfasserin aut Turid Follestad verfasserin aut Kristin Sand verfasserin aut Merete Mack verfasserin aut Karina Standahl Olsen verfasserin aut Anne Gøril Lundemo verfasserin aut Jens Gustav Iversen verfasserin aut Hans Einar Krokan verfasserin aut Svanhild Arentz Schønberg verfasserin aut In Journal of Lipid Research Elsevier, 2021 49(2008), 10, Seite 2089-2100 (DE-627)26601593X (DE-600)1466675-3 15397262 nnns volume:49 year:2008 number:10 pages:2089-2100 https://doi.org/10.1194/jlr.M700389-JLR200 kostenfrei https://doaj.org/article/fb81cf3aee5e4ebbad547dc6be7d20e4 kostenfrei http://www.sciencedirect.com/science/article/pii/S0022227520346320 kostenfrei https://doaj.org/toc/0022-2275 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2006 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 49 2008 10 2089-2100 |
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10.1194/jlr.M700389-JLR200 doi (DE-627)DOAJ055454739 (DE-599)DOAJfb81cf3aee5e4ebbad547dc6be7d20e4 DE-627 ger DE-627 rakwb eng QD415-436 Caroline Hild Jakobsen verfasserin aut DHA induces ER stress and growth arrest in human colon cancer cells: associations with cholesterol and calcium homeostasis *s⃞ 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Polyunsaturated fatty acids (PUFAs) are normal constituents of the diet, but have properties different from other fatty acids (e.g., through generation of signaling molecules). N-3 PUFAs reduce cancer cell growth, but no unified mechanism has been identified. We show that docosahexaenoic acid (DHA; 22:6 n-3) causes extensive changes in gene expression patterns at mRNA level in the colon cancer cell line SW620. Early changes include unfolded protein response (UPR) and increased levels of phosphorylated eIF2α as verified at protein level. The latter is considered a hallmark of endoplasmic reticulum (ER) stress and is abundantly present already after 3 h. It may coordinate many of the downstream changes observed, including signaling pathways for cell cycle arrest/apoptosis, calcium homeostasis, cholesterol metabolism, ubiquitination, and proteasomal degradation. Also, eicosapentaenoic acid (EPA), but not oleic acid (OA), induced key mediators of ER stress and UPR at protein level. Accumulation of esterified cholesterol was not compensated for by increased total levels of cholesterol, and mRNAs for cholesterol biosynthesis as well as de novo synthesis of cholesterol were reduced. These results suggest that cytotoxic effects of DHA are associated with signaling pathways involving lipid metabolism and ER stress. gene expression phosphorylated eIF2α antioxidant response heat shock response cytosolic free Ca2+ cell cycle Biochemistry Gro Leite Størvold verfasserin aut Hilde Bremseth verfasserin aut Turid Follestad verfasserin aut Kristin Sand verfasserin aut Merete Mack verfasserin aut Karina Standahl Olsen verfasserin aut Anne Gøril Lundemo verfasserin aut Jens Gustav Iversen verfasserin aut Hans Einar Krokan verfasserin aut Svanhild Arentz Schønberg verfasserin aut In Journal of Lipid Research Elsevier, 2021 49(2008), 10, Seite 2089-2100 (DE-627)26601593X (DE-600)1466675-3 15397262 nnns volume:49 year:2008 number:10 pages:2089-2100 https://doi.org/10.1194/jlr.M700389-JLR200 kostenfrei https://doaj.org/article/fb81cf3aee5e4ebbad547dc6be7d20e4 kostenfrei http://www.sciencedirect.com/science/article/pii/S0022227520346320 kostenfrei https://doaj.org/toc/0022-2275 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2006 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 49 2008 10 2089-2100 |
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10.1194/jlr.M700389-JLR200 doi (DE-627)DOAJ055454739 (DE-599)DOAJfb81cf3aee5e4ebbad547dc6be7d20e4 DE-627 ger DE-627 rakwb eng QD415-436 Caroline Hild Jakobsen verfasserin aut DHA induces ER stress and growth arrest in human colon cancer cells: associations with cholesterol and calcium homeostasis *s⃞ 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Polyunsaturated fatty acids (PUFAs) are normal constituents of the diet, but have properties different from other fatty acids (e.g., through generation of signaling molecules). N-3 PUFAs reduce cancer cell growth, but no unified mechanism has been identified. We show that docosahexaenoic acid (DHA; 22:6 n-3) causes extensive changes in gene expression patterns at mRNA level in the colon cancer cell line SW620. Early changes include unfolded protein response (UPR) and increased levels of phosphorylated eIF2α as verified at protein level. The latter is considered a hallmark of endoplasmic reticulum (ER) stress and is abundantly present already after 3 h. It may coordinate many of the downstream changes observed, including signaling pathways for cell cycle arrest/apoptosis, calcium homeostasis, cholesterol metabolism, ubiquitination, and proteasomal degradation. Also, eicosapentaenoic acid (EPA), but not oleic acid (OA), induced key mediators of ER stress and UPR at protein level. Accumulation of esterified cholesterol was not compensated for by increased total levels of cholesterol, and mRNAs for cholesterol biosynthesis as well as de novo synthesis of cholesterol were reduced. These results suggest that cytotoxic effects of DHA are associated with signaling pathways involving lipid metabolism and ER stress. gene expression phosphorylated eIF2α antioxidant response heat shock response cytosolic free Ca2+ cell cycle Biochemistry Gro Leite Størvold verfasserin aut Hilde Bremseth verfasserin aut Turid Follestad verfasserin aut Kristin Sand verfasserin aut Merete Mack verfasserin aut Karina Standahl Olsen verfasserin aut Anne Gøril Lundemo verfasserin aut Jens Gustav Iversen verfasserin aut Hans Einar Krokan verfasserin aut Svanhild Arentz Schønberg verfasserin aut In Journal of Lipid Research Elsevier, 2021 49(2008), 10, Seite 2089-2100 (DE-627)26601593X (DE-600)1466675-3 15397262 nnns volume:49 year:2008 number:10 pages:2089-2100 https://doi.org/10.1194/jlr.M700389-JLR200 kostenfrei https://doaj.org/article/fb81cf3aee5e4ebbad547dc6be7d20e4 kostenfrei http://www.sciencedirect.com/science/article/pii/S0022227520346320 kostenfrei https://doaj.org/toc/0022-2275 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2006 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 49 2008 10 2089-2100 |
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10.1194/jlr.M700389-JLR200 doi (DE-627)DOAJ055454739 (DE-599)DOAJfb81cf3aee5e4ebbad547dc6be7d20e4 DE-627 ger DE-627 rakwb eng QD415-436 Caroline Hild Jakobsen verfasserin aut DHA induces ER stress and growth arrest in human colon cancer cells: associations with cholesterol and calcium homeostasis *s⃞ 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Polyunsaturated fatty acids (PUFAs) are normal constituents of the diet, but have properties different from other fatty acids (e.g., through generation of signaling molecules). N-3 PUFAs reduce cancer cell growth, but no unified mechanism has been identified. We show that docosahexaenoic acid (DHA; 22:6 n-3) causes extensive changes in gene expression patterns at mRNA level in the colon cancer cell line SW620. Early changes include unfolded protein response (UPR) and increased levels of phosphorylated eIF2α as verified at protein level. The latter is considered a hallmark of endoplasmic reticulum (ER) stress and is abundantly present already after 3 h. It may coordinate many of the downstream changes observed, including signaling pathways for cell cycle arrest/apoptosis, calcium homeostasis, cholesterol metabolism, ubiquitination, and proteasomal degradation. Also, eicosapentaenoic acid (EPA), but not oleic acid (OA), induced key mediators of ER stress and UPR at protein level. Accumulation of esterified cholesterol was not compensated for by increased total levels of cholesterol, and mRNAs for cholesterol biosynthesis as well as de novo synthesis of cholesterol were reduced. These results suggest that cytotoxic effects of DHA are associated with signaling pathways involving lipid metabolism and ER stress. gene expression phosphorylated eIF2α antioxidant response heat shock response cytosolic free Ca2+ cell cycle Biochemistry Gro Leite Størvold verfasserin aut Hilde Bremseth verfasserin aut Turid Follestad verfasserin aut Kristin Sand verfasserin aut Merete Mack verfasserin aut Karina Standahl Olsen verfasserin aut Anne Gøril Lundemo verfasserin aut Jens Gustav Iversen verfasserin aut Hans Einar Krokan verfasserin aut Svanhild Arentz Schønberg verfasserin aut In Journal of Lipid Research Elsevier, 2021 49(2008), 10, Seite 2089-2100 (DE-627)26601593X (DE-600)1466675-3 15397262 nnns volume:49 year:2008 number:10 pages:2089-2100 https://doi.org/10.1194/jlr.M700389-JLR200 kostenfrei https://doaj.org/article/fb81cf3aee5e4ebbad547dc6be7d20e4 kostenfrei http://www.sciencedirect.com/science/article/pii/S0022227520346320 kostenfrei https://doaj.org/toc/0022-2275 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2006 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 49 2008 10 2089-2100 |
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Polyunsaturated fatty acids (PUFAs) are normal constituents of the diet, but have properties different from other fatty acids (e.g., through generation of signaling molecules). N-3 PUFAs reduce cancer cell growth, but no unified mechanism has been identified. We show that docosahexaenoic acid (DHA; 22:6 n-3) causes extensive changes in gene expression patterns at mRNA level in the colon cancer cell line SW620. Early changes include unfolded protein response (UPR) and increased levels of phosphorylated eIF2α as verified at protein level. The latter is considered a hallmark of endoplasmic reticulum (ER) stress and is abundantly present already after 3 h. It may coordinate many of the downstream changes observed, including signaling pathways for cell cycle arrest/apoptosis, calcium homeostasis, cholesterol metabolism, ubiquitination, and proteasomal degradation. Also, eicosapentaenoic acid (EPA), but not oleic acid (OA), induced key mediators of ER stress and UPR at protein level. Accumulation of esterified cholesterol was not compensated for by increased total levels of cholesterol, and mRNAs for cholesterol biosynthesis as well as de novo synthesis of cholesterol were reduced. These results suggest that cytotoxic effects of DHA are associated with signaling pathways involving lipid metabolism and ER stress. |
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Polyunsaturated fatty acids (PUFAs) are normal constituents of the diet, but have properties different from other fatty acids (e.g., through generation of signaling molecules). N-3 PUFAs reduce cancer cell growth, but no unified mechanism has been identified. We show that docosahexaenoic acid (DHA; 22:6 n-3) causes extensive changes in gene expression patterns at mRNA level in the colon cancer cell line SW620. Early changes include unfolded protein response (UPR) and increased levels of phosphorylated eIF2α as verified at protein level. The latter is considered a hallmark of endoplasmic reticulum (ER) stress and is abundantly present already after 3 h. It may coordinate many of the downstream changes observed, including signaling pathways for cell cycle arrest/apoptosis, calcium homeostasis, cholesterol metabolism, ubiquitination, and proteasomal degradation. Also, eicosapentaenoic acid (EPA), but not oleic acid (OA), induced key mediators of ER stress and UPR at protein level. Accumulation of esterified cholesterol was not compensated for by increased total levels of cholesterol, and mRNAs for cholesterol biosynthesis as well as de novo synthesis of cholesterol were reduced. These results suggest that cytotoxic effects of DHA are associated with signaling pathways involving lipid metabolism and ER stress. |
| abstract_unstemmed |
Polyunsaturated fatty acids (PUFAs) are normal constituents of the diet, but have properties different from other fatty acids (e.g., through generation of signaling molecules). N-3 PUFAs reduce cancer cell growth, but no unified mechanism has been identified. We show that docosahexaenoic acid (DHA; 22:6 n-3) causes extensive changes in gene expression patterns at mRNA level in the colon cancer cell line SW620. Early changes include unfolded protein response (UPR) and increased levels of phosphorylated eIF2α as verified at protein level. The latter is considered a hallmark of endoplasmic reticulum (ER) stress and is abundantly present already after 3 h. It may coordinate many of the downstream changes observed, including signaling pathways for cell cycle arrest/apoptosis, calcium homeostasis, cholesterol metabolism, ubiquitination, and proteasomal degradation. Also, eicosapentaenoic acid (EPA), but not oleic acid (OA), induced key mediators of ER stress and UPR at protein level. Accumulation of esterified cholesterol was not compensated for by increased total levels of cholesterol, and mRNAs for cholesterol biosynthesis as well as de novo synthesis of cholesterol were reduced. These results suggest that cytotoxic effects of DHA are associated with signaling pathways involving lipid metabolism and ER stress. |
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DHA induces ER stress and growth arrest in human colon cancer cells: associations with cholesterol and calcium homeostasis *s⃞ |
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https://doi.org/10.1194/jlr.M700389-JLR200 https://doaj.org/article/fb81cf3aee5e4ebbad547dc6be7d20e4 http://www.sciencedirect.com/science/article/pii/S0022227520346320 https://doaj.org/toc/0022-2275 |
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Gro Leite Størvold Hilde Bremseth Turid Follestad Kristin Sand Merete Mack Karina Standahl Olsen Anne Gøril Lundemo Jens Gustav Iversen Hans Einar Krokan Svanhild Arentz Schønberg |
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