Generation of Monoclonal Antibodies for Sensitive Detection of Pro-Inflammatory Protein S100A9

The calcium-binding protein S100A9 regulates inflammatory processes and the immune response. It is overexpressed in a variety of inflammatory and oncologic conditions. In this study, we produced a recombinant human S100A9 (hS100A9) antigen with high yield and purity and used it to generate a hybrido...
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Autor*in:	Eun-Jung Kim [verfasserIn] Gyu-Min Im [verfasserIn] Chang-Soo Lee [verfasserIn] Yun-Gon Kim [verfasserIn] Byoung Joon Ko [verfasserIn] Hee-Jin Jeong [verfasserIn] Byung-Gee Kim [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	S100A9 recombinant protein monoclonal antibody inflammation

Übergeordnetes Werk:	In: Applied Sciences - MDPI AG, 2012, 11(2021), 10, p 4659
Übergeordnetes Werk:	volume:11 ; year:2021 ; number:10, p 4659

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/app11104659

Katalog-ID:	DOAJ055616925

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allfields	10.3390/app11104659 doi (DE-627)DOAJ055616925 (DE-599)DOAJ176fcb513c214894bc2bc82a5ac7ee82 DE-627 ger DE-627 rakwb eng TA1-2040 QH301-705.5 QC1-999 QD1-999 Eun-Jung Kim verfasserin aut Generation of Monoclonal Antibodies for Sensitive Detection of Pro-Inflammatory Protein S100A9 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The calcium-binding protein S100A9 regulates inflammatory processes and the immune response. It is overexpressed in a variety of inflammatory and oncologic conditions. In this study, we produced a recombinant human S100A9 (hS100A9) antigen with high yield and purity and used it to generate a hybridoma cell culture-based monoclonal anti-hS100A9 antibody. We selected five anti-hS100A9 antibodies from cell supernatants that showed high antigen binding efficiency and identified the nucleotide sequences of three antibodies: two with high effective concentration values and one with the lowest value. The antigen and antibody development procedures described herein are useful for producing large amounts of monoclonal antibodies against hS100A9 and other antigens of interest. The nucleotide sequences of the anti-hS100A9 monoclonal antibody revealed herein will be helpful in the generation of recombinant antibodies or antibody fragments against hS100A9. S100A9 recombinant protein monoclonal antibody inflammation Technology T Engineering (General). Civil engineering (General) Biology (General) Physics Chemistry Gyu-Min Im verfasserin aut Chang-Soo Lee verfasserin aut Yun-Gon Kim verfasserin aut Byoung Joon Ko verfasserin aut Hee-Jin Jeong verfasserin aut Byung-Gee Kim verfasserin aut In Applied Sciences MDPI AG, 2012 11(2021), 10, p 4659 (DE-627)737287640 (DE-600)2704225-X 20763417 nnns volume:11 year:2021 number:10, p 4659 https://doi.org/10.3390/app11104659 kostenfrei https://doaj.org/article/176fcb513c214894bc2bc82a5ac7ee82 kostenfrei https://www.mdpi.com/2076-3417/11/10/4659 kostenfrei https://doaj.org/toc/2076-3417 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2021 10, p 4659
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allfieldsSound	10.3390/app11104659 doi (DE-627)DOAJ055616925 (DE-599)DOAJ176fcb513c214894bc2bc82a5ac7ee82 DE-627 ger DE-627 rakwb eng TA1-2040 QH301-705.5 QC1-999 QD1-999 Eun-Jung Kim verfasserin aut Generation of Monoclonal Antibodies for Sensitive Detection of Pro-Inflammatory Protein S100A9 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The calcium-binding protein S100A9 regulates inflammatory processes and the immune response. It is overexpressed in a variety of inflammatory and oncologic conditions. In this study, we produced a recombinant human S100A9 (hS100A9) antigen with high yield and purity and used it to generate a hybridoma cell culture-based monoclonal anti-hS100A9 antibody. We selected five anti-hS100A9 antibodies from cell supernatants that showed high antigen binding efficiency and identified the nucleotide sequences of three antibodies: two with high effective concentration values and one with the lowest value. The antigen and antibody development procedures described herein are useful for producing large amounts of monoclonal antibodies against hS100A9 and other antigens of interest. The nucleotide sequences of the anti-hS100A9 monoclonal antibody revealed herein will be helpful in the generation of recombinant antibodies or antibody fragments against hS100A9. S100A9 recombinant protein monoclonal antibody inflammation Technology T Engineering (General). Civil engineering (General) Biology (General) Physics Chemistry Gyu-Min Im verfasserin aut Chang-Soo Lee verfasserin aut Yun-Gon Kim verfasserin aut Byoung Joon Ko verfasserin aut Hee-Jin Jeong verfasserin aut Byung-Gee Kim verfasserin aut In Applied Sciences MDPI AG, 2012 11(2021), 10, p 4659 (DE-627)737287640 (DE-600)2704225-X 20763417 nnns volume:11 year:2021 number:10, p 4659 https://doi.org/10.3390/app11104659 kostenfrei https://doaj.org/article/176fcb513c214894bc2bc82a5ac7ee82 kostenfrei https://www.mdpi.com/2076-3417/11/10/4659 kostenfrei https://doaj.org/toc/2076-3417 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 11 2021 10, p 4659
language	English
source	In Applied Sciences 11(2021), 10, p 4659 volume:11 year:2021 number:10, p 4659
sourceStr	In Applied Sciences 11(2021), 10, p 4659 volume:11 year:2021 number:10, p 4659
format_phy_str_mv	Article
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topic_facet	S100A9 recombinant protein monoclonal antibody inflammation Technology T Engineering (General). Civil engineering (General) Biology (General) Physics Chemistry
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authorswithroles_txt_mv	Eun-Jung Kim @@aut@@ Gyu-Min Im @@aut@@ Chang-Soo Lee @@aut@@ Yun-Gon Kim @@aut@@ Byoung Joon Ko @@aut@@ Hee-Jin Jeong @@aut@@ Byung-Gee Kim @@aut@@
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callnumber-first	T - Technology
author	Eun-Jung Kim
spellingShingle	Eun-Jung Kim misc TA1-2040 misc QH301-705.5 misc QC1-999 misc QD1-999 misc S100A9 misc recombinant protein misc monoclonal antibody misc inflammation misc Technology misc T misc Engineering (General). Civil engineering (General) misc Biology (General) misc Physics misc Chemistry Generation of Monoclonal Antibodies for Sensitive Detection of Pro-Inflammatory Protein S100A9
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topic_title	TA1-2040 QH301-705.5 QC1-999 QD1-999 Generation of Monoclonal Antibodies for Sensitive Detection of Pro-Inflammatory Protein S100A9 S100A9 recombinant protein monoclonal antibody inflammation
topic	misc TA1-2040 misc QH301-705.5 misc QC1-999 misc QD1-999 misc S100A9 misc recombinant protein misc monoclonal antibody misc inflammation misc Technology misc T misc Engineering (General). Civil engineering (General) misc Biology (General) misc Physics misc Chemistry
topic_unstemmed	misc TA1-2040 misc QH301-705.5 misc QC1-999 misc QD1-999 misc S100A9 misc recombinant protein misc monoclonal antibody misc inflammation misc Technology misc T misc Engineering (General). Civil engineering (General) misc Biology (General) misc Physics misc Chemistry
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title	Generation of Monoclonal Antibodies for Sensitive Detection of Pro-Inflammatory Protein S100A9
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title_full	Generation of Monoclonal Antibodies for Sensitive Detection of Pro-Inflammatory Protein S100A9
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title_sort	generation of monoclonal antibodies for sensitive detection of pro-inflammatory protein s100a9
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title_auth	Generation of Monoclonal Antibodies for Sensitive Detection of Pro-Inflammatory Protein S100A9
abstract	The calcium-binding protein S100A9 regulates inflammatory processes and the immune response. It is overexpressed in a variety of inflammatory and oncologic conditions. In this study, we produced a recombinant human S100A9 (hS100A9) antigen with high yield and purity and used it to generate a hybridoma cell culture-based monoclonal anti-hS100A9 antibody. We selected five anti-hS100A9 antibodies from cell supernatants that showed high antigen binding efficiency and identified the nucleotide sequences of three antibodies: two with high effective concentration values and one with the lowest value. The antigen and antibody development procedures described herein are useful for producing large amounts of monoclonal antibodies against hS100A9 and other antigens of interest. The nucleotide sequences of the anti-hS100A9 monoclonal antibody revealed herein will be helpful in the generation of recombinant antibodies or antibody fragments against hS100A9.
abstractGer	The calcium-binding protein S100A9 regulates inflammatory processes and the immune response. It is overexpressed in a variety of inflammatory and oncologic conditions. In this study, we produced a recombinant human S100A9 (hS100A9) antigen with high yield and purity and used it to generate a hybridoma cell culture-based monoclonal anti-hS100A9 antibody. We selected five anti-hS100A9 antibodies from cell supernatants that showed high antigen binding efficiency and identified the nucleotide sequences of three antibodies: two with high effective concentration values and one with the lowest value. The antigen and antibody development procedures described herein are useful for producing large amounts of monoclonal antibodies against hS100A9 and other antigens of interest. The nucleotide sequences of the anti-hS100A9 monoclonal antibody revealed herein will be helpful in the generation of recombinant antibodies or antibody fragments against hS100A9.
abstract_unstemmed	The calcium-binding protein S100A9 regulates inflammatory processes and the immune response. It is overexpressed in a variety of inflammatory and oncologic conditions. In this study, we produced a recombinant human S100A9 (hS100A9) antigen with high yield and purity and used it to generate a hybridoma cell culture-based monoclonal anti-hS100A9 antibody. We selected five anti-hS100A9 antibodies from cell supernatants that showed high antigen binding efficiency and identified the nucleotide sequences of three antibodies: two with high effective concentration values and one with the lowest value. The antigen and antibody development procedures described herein are useful for producing large amounts of monoclonal antibodies against hS100A9 and other antigens of interest. The nucleotide sequences of the anti-hS100A9 monoclonal antibody revealed herein will be helpful in the generation of recombinant antibodies or antibody fragments against hS100A9.
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title_short	Generation of Monoclonal Antibodies for Sensitive Detection of Pro-Inflammatory Protein S100A9
url	https://doi.org/10.3390/app11104659 https://doaj.org/article/176fcb513c214894bc2bc82a5ac7ee82 https://www.mdpi.com/2076-3417/11/10/4659 https://doaj.org/toc/2076-3417
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Generation of Monoclonal Antibodies for Sensitive Detection of Pro-Inflammatory Protein S100A9

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