Direct Assay of miR-223, miR-145, and miR-16 by a Novel Method of Mimic PCR in Serum of Breast Cancer Patients

The role of microRNAs (miRs) is shown as a biomarker whose expression level changes have been associated with cancer development and progression. Circulating miRNAs have been postulated as biomarkers for breast cancer. Detection and diagnosis of miRNA markers could provide an improved and sensitive...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Elham Ghazizadeh [verfasserIn] Faranak Hadi [verfasserIn] Maryam Zare [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2016

Schlagwörter:	biomarker micro rna mimic pcr breast cancer

Übergeordnetes Werk:	In: Journal of Genetic Resources - University of Mazandaran, 2021, 2(2016), 2, Seite 98-108
Übergeordnetes Werk:	volume:2 ; year:2016 ; number:2 ; pages:98-108

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc Journal toc

DOI / URN:	10.22080/jgr.2017.12953.1068

Katalog-ID:	DOAJ055813569

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allfieldsSound	10.22080/jgr.2017.12953.1068 doi (DE-627)DOAJ055813569 (DE-599)DOAJ0dc864074bf64c42993fe5f0f7897391 DE-627 ger DE-627 rakwb eng QH426-470 Elham Ghazizadeh verfasserin aut Direct Assay of miR-223, miR-145, and miR-16 by a Novel Method of Mimic PCR in Serum of Breast Cancer Patients 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The role of microRNAs (miRs) is shown as a biomarker whose expression level changes have been associated with cancer development and progression. Circulating miRNAs have been postulated as biomarkers for breast cancer. Detection and diagnosis of miRNA markers could provide an improved and sensitive method in the clinical application. In this study, we applied and improved the effective approach by coupling a deoxyuridine-incorporated RT oligonucleotide with a secondary structure and a mimic PCR for assessment and direct detection of circulating miRs in serum. We designed mut miR-16, mut miR-145, and mut miR-223 as mimic miRs. These mimic miRs were used in RT-PCR for detection and quantification assay. As normalization of these mut miRs was performed with themselves, our mimic construct with 80 bp of these miRs shows the best normalization for this method in blood samples. This assay was tested in serum from 15 patients with different stages of breast cancer and 10 healthy female donors. According to our results, using a combination of miR-16 and miR-145 could represent as one of the best biomarker (p biomarker micro rna mimic pcr breast cancer Genetics Faranak Hadi verfasserin aut Maryam Zare verfasserin aut In Journal of Genetic Resources University of Mazandaran, 2021 2(2016), 2, Seite 98-108 (DE-627)1767903766 25882589 nnns volume:2 year:2016 number:2 pages:98-108 https://doi.org/10.22080/jgr.2017.12953.1068 kostenfrei https://doaj.org/article/0dc864074bf64c42993fe5f0f7897391 kostenfrei http://sc.journals.umz.ac.ir/article_1659_caa74ab91b02ca38fd6cc5a6298035ba.pdf kostenfrei https://doaj.org/toc/2423-4257 Journal toc kostenfrei https://doaj.org/toc/2588-2589 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 2 2016 2 98-108
language	English
source	In Journal of Genetic Resources 2(2016), 2, Seite 98-108 volume:2 year:2016 number:2 pages:98-108
sourceStr	In Journal of Genetic Resources 2(2016), 2, Seite 98-108 volume:2 year:2016 number:2 pages:98-108
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	biomarker micro rna mimic pcr breast cancer Genetics
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container_title	Journal of Genetic Resources
authorswithroles_txt_mv	Elham Ghazizadeh @@aut@@ Faranak Hadi @@aut@@ Maryam Zare @@aut@@
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callnumber-first	Q - Science
author	Elham Ghazizadeh
spellingShingle	Elham Ghazizadeh misc QH426-470 misc biomarker misc micro rna misc mimic pcr misc breast cancer misc Genetics Direct Assay of miR-223, miR-145, and miR-16 by a Novel Method of Mimic PCR in Serum of Breast Cancer Patients
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topic_title	QH426-470 Direct Assay of miR-223, miR-145, and miR-16 by a Novel Method of Mimic PCR in Serum of Breast Cancer Patients biomarker micro rna mimic pcr breast cancer
topic	misc QH426-470 misc biomarker misc micro rna misc mimic pcr misc breast cancer misc Genetics
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title	Direct Assay of miR-223, miR-145, and miR-16 by a Novel Method of Mimic PCR in Serum of Breast Cancer Patients
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title_full	Direct Assay of miR-223, miR-145, and miR-16 by a Novel Method of Mimic PCR in Serum of Breast Cancer Patients
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title_sort	direct assay of mir-223, mir-145, and mir-16 by a novel method of mimic pcr in serum of breast cancer patients
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title_auth	Direct Assay of miR-223, miR-145, and miR-16 by a Novel Method of Mimic PCR in Serum of Breast Cancer Patients
abstract	The role of microRNAs (miRs) is shown as a biomarker whose expression level changes have been associated with cancer development and progression. Circulating miRNAs have been postulated as biomarkers for breast cancer. Detection and diagnosis of miRNA markers could provide an improved and sensitive method in the clinical application. In this study, we applied and improved the effective approach by coupling a deoxyuridine-incorporated RT oligonucleotide with a secondary structure and a mimic PCR for assessment and direct detection of circulating miRs in serum. We designed mut miR-16, mut miR-145, and mut miR-223 as mimic miRs. These mimic miRs were used in RT-PCR for detection and quantification assay. As normalization of these mut miRs was performed with themselves, our mimic construct with 80 bp of these miRs shows the best normalization for this method in blood samples. This assay was tested in serum from 15 patients with different stages of breast cancer and 10 healthy female donors. According to our results, using a combination of miR-16 and miR-145 could represent as one of the best biomarker (p
abstractGer	The role of microRNAs (miRs) is shown as a biomarker whose expression level changes have been associated with cancer development and progression. Circulating miRNAs have been postulated as biomarkers for breast cancer. Detection and diagnosis of miRNA markers could provide an improved and sensitive method in the clinical application. In this study, we applied and improved the effective approach by coupling a deoxyuridine-incorporated RT oligonucleotide with a secondary structure and a mimic PCR for assessment and direct detection of circulating miRs in serum. We designed mut miR-16, mut miR-145, and mut miR-223 as mimic miRs. These mimic miRs were used in RT-PCR for detection and quantification assay. As normalization of these mut miRs was performed with themselves, our mimic construct with 80 bp of these miRs shows the best normalization for this method in blood samples. This assay was tested in serum from 15 patients with different stages of breast cancer and 10 healthy female donors. According to our results, using a combination of miR-16 and miR-145 could represent as one of the best biomarker (p
abstract_unstemmed	The role of microRNAs (miRs) is shown as a biomarker whose expression level changes have been associated with cancer development and progression. Circulating miRNAs have been postulated as biomarkers for breast cancer. Detection and diagnosis of miRNA markers could provide an improved and sensitive method in the clinical application. In this study, we applied and improved the effective approach by coupling a deoxyuridine-incorporated RT oligonucleotide with a secondary structure and a mimic PCR for assessment and direct detection of circulating miRs in serum. We designed mut miR-16, mut miR-145, and mut miR-223 as mimic miRs. These mimic miRs were used in RT-PCR for detection and quantification assay. As normalization of these mut miRs was performed with themselves, our mimic construct with 80 bp of these miRs shows the best normalization for this method in blood samples. This assay was tested in serum from 15 patients with different stages of breast cancer and 10 healthy female donors. According to our results, using a combination of miR-16 and miR-145 could represent as one of the best biomarker (p
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title_short	Direct Assay of miR-223, miR-145, and miR-16 by a Novel Method of Mimic PCR in Serum of Breast Cancer Patients
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Direct Assay of miR-223, miR-145, and miR-16 by a Novel Method of Mimic PCR in Serum of Breast Cancer Patients

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