Multi-Functional MPT Protein as a Therapeutic Agent against <i<Mycobacterium tuberculosis</i<

<i<Mycobacterium tuberculosis</i< (MTB), the causative agent of tuberculosis (TB), avoids the host immune system through its virulence factors. MPT63 and MPT64 are the virulence factors secreted by MTB which regulate host proteins for the survival and proliferation of MTB in the host. He...
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Autor*in:	Jae-Sung Kim [verfasserIn] Euni Cho [verfasserIn] Seok-Jun Mun [verfasserIn] Sojin Kim [verfasserIn] Sun-Young Kim [verfasserIn] Dong-Gyu Kim [verfasserIn] Wooic Son [verfasserIn] Hye-In Jeon [verfasserIn] Hyo-Keun Kim [verfasserIn] Young-Jin Jeong [verfasserIn] Sein Jang [verfasserIn] Hyun-Sung Kim [verfasserIn] Chul-Su Yang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	MPT peptide TBK1 p47phox hexokinase 2 macrophages

Übergeordnetes Werk:	In: Biomedicines - MDPI AG, 2014, 9(2021), 5, p 545
Übergeordnetes Werk:	volume:9 ; year:2021 ; number:5, p 545

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/biomedicines9050545

Katalog-ID:	DOAJ055905293

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spelling	10.3390/biomedicines9050545 doi (DE-627)DOAJ055905293 (DE-599)DOAJ81dfed782e084defbedc49002d7a5238 DE-627 ger DE-627 rakwb eng QH301-705.5 Jae-Sung Kim verfasserin aut Multi-Functional MPT Protein as a Therapeutic Agent against <i<Mycobacterium tuberculosis</i< 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Mycobacterium tuberculosis</i< (MTB), the causative agent of tuberculosis (TB), avoids the host immune system through its virulence factors. MPT63 and MPT64 are the virulence factors secreted by MTB which regulate host proteins for the survival and proliferation of MTB in the host. Here, we found that MPT63 bound directly with TBK1 and p47phox, whereas MPT64 interacted with TBK1 and HK2. We constructed a MPT63/64-derived multifunctional recombinant protein (rMPT) that was able to interact with TBK1, p47phox, or HK2. rMPT was shown to regulate IFN-β levels and increase inflammation and concentration of reactive oxygen species (ROS), while targeting macrophages and killing MTB, both in vitro and in vivo. Furthermore, the identification of the role of rMPT against MTB was achieved via vaccination in a mouse model. Taken together, we here present rMPT, which, by regulating important immune signaling systems, can be considered an effective vaccine or therapeutic agent against MTB. <i<Mycobacterium tuberculosis</i< MPT peptide TBK1 p47phox hexokinase 2 macrophages Biology (General) Euni Cho verfasserin aut Seok-Jun Mun verfasserin aut Sojin Kim verfasserin aut Sun-Young Kim verfasserin aut Dong-Gyu Kim verfasserin aut Wooic Son verfasserin aut Hye-In Jeon verfasserin aut Hyo-Keun Kim verfasserin aut Young-Jin Jeong verfasserin aut Sein Jang verfasserin aut Hyun-Sung Kim verfasserin aut Chul-Su Yang verfasserin aut In Biomedicines MDPI AG, 2014 9(2021), 5, p 545 (DE-627)750370483 (DE-600)2720867-9 22279059 nnns volume:9 year:2021 number:5, p 545 https://doi.org/10.3390/biomedicines9050545 kostenfrei https://doaj.org/article/81dfed782e084defbedc49002d7a5238 kostenfrei https://www.mdpi.com/2227-9059/9/5/545 kostenfrei https://doaj.org/toc/2227-9059 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 5, p 545
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allfieldsGer	10.3390/biomedicines9050545 doi (DE-627)DOAJ055905293 (DE-599)DOAJ81dfed782e084defbedc49002d7a5238 DE-627 ger DE-627 rakwb eng QH301-705.5 Jae-Sung Kim verfasserin aut Multi-Functional MPT Protein as a Therapeutic Agent against <i<Mycobacterium tuberculosis</i< 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Mycobacterium tuberculosis</i< (MTB), the causative agent of tuberculosis (TB), avoids the host immune system through its virulence factors. MPT63 and MPT64 are the virulence factors secreted by MTB which regulate host proteins for the survival and proliferation of MTB in the host. Here, we found that MPT63 bound directly with TBK1 and p47phox, whereas MPT64 interacted with TBK1 and HK2. We constructed a MPT63/64-derived multifunctional recombinant protein (rMPT) that was able to interact with TBK1, p47phox, or HK2. rMPT was shown to regulate IFN-β levels and increase inflammation and concentration of reactive oxygen species (ROS), while targeting macrophages and killing MTB, both in vitro and in vivo. Furthermore, the identification of the role of rMPT against MTB was achieved via vaccination in a mouse model. Taken together, we here present rMPT, which, by regulating important immune signaling systems, can be considered an effective vaccine or therapeutic agent against MTB. <i<Mycobacterium tuberculosis</i< MPT peptide TBK1 p47phox hexokinase 2 macrophages Biology (General) Euni Cho verfasserin aut Seok-Jun Mun verfasserin aut Sojin Kim verfasserin aut Sun-Young Kim verfasserin aut Dong-Gyu Kim verfasserin aut Wooic Son verfasserin aut Hye-In Jeon verfasserin aut Hyo-Keun Kim verfasserin aut Young-Jin Jeong verfasserin aut Sein Jang verfasserin aut Hyun-Sung Kim verfasserin aut Chul-Su Yang verfasserin aut In Biomedicines MDPI AG, 2014 9(2021), 5, p 545 (DE-627)750370483 (DE-600)2720867-9 22279059 nnns volume:9 year:2021 number:5, p 545 https://doi.org/10.3390/biomedicines9050545 kostenfrei https://doaj.org/article/81dfed782e084defbedc49002d7a5238 kostenfrei https://www.mdpi.com/2227-9059/9/5/545 kostenfrei https://doaj.org/toc/2227-9059 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 5, p 545
allfieldsSound	10.3390/biomedicines9050545 doi (DE-627)DOAJ055905293 (DE-599)DOAJ81dfed782e084defbedc49002d7a5238 DE-627 ger DE-627 rakwb eng QH301-705.5 Jae-Sung Kim verfasserin aut Multi-Functional MPT Protein as a Therapeutic Agent against <i<Mycobacterium tuberculosis</i< 2021 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier <i<Mycobacterium tuberculosis</i< (MTB), the causative agent of tuberculosis (TB), avoids the host immune system through its virulence factors. MPT63 and MPT64 are the virulence factors secreted by MTB which regulate host proteins for the survival and proliferation of MTB in the host. Here, we found that MPT63 bound directly with TBK1 and p47phox, whereas MPT64 interacted with TBK1 and HK2. We constructed a MPT63/64-derived multifunctional recombinant protein (rMPT) that was able to interact with TBK1, p47phox, or HK2. rMPT was shown to regulate IFN-β levels and increase inflammation and concentration of reactive oxygen species (ROS), while targeting macrophages and killing MTB, both in vitro and in vivo. Furthermore, the identification of the role of rMPT against MTB was achieved via vaccination in a mouse model. Taken together, we here present rMPT, which, by regulating important immune signaling systems, can be considered an effective vaccine or therapeutic agent against MTB. <i<Mycobacterium tuberculosis</i< MPT peptide TBK1 p47phox hexokinase 2 macrophages Biology (General) Euni Cho verfasserin aut Seok-Jun Mun verfasserin aut Sojin Kim verfasserin aut Sun-Young Kim verfasserin aut Dong-Gyu Kim verfasserin aut Wooic Son verfasserin aut Hye-In Jeon verfasserin aut Hyo-Keun Kim verfasserin aut Young-Jin Jeong verfasserin aut Sein Jang verfasserin aut Hyun-Sung Kim verfasserin aut Chul-Su Yang verfasserin aut In Biomedicines MDPI AG, 2014 9(2021), 5, p 545 (DE-627)750370483 (DE-600)2720867-9 22279059 nnns volume:9 year:2021 number:5, p 545 https://doi.org/10.3390/biomedicines9050545 kostenfrei https://doaj.org/article/81dfed782e084defbedc49002d7a5238 kostenfrei https://www.mdpi.com/2227-9059/9/5/545 kostenfrei https://doaj.org/toc/2227-9059 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 9 2021 5, p 545
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callnumber-first	Q - Science
author	Jae-Sung Kim
spellingShingle	Jae-Sung Kim misc QH301-705.5 misc <i<Mycobacterium tuberculosis</i< misc MPT peptide misc TBK1 misc p47phox misc hexokinase 2 misc macrophages misc Biology (General) Multi-Functional MPT Protein as a Therapeutic Agent against <i<Mycobacterium tuberculosis</i<
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topic_title	QH301-705.5 Multi-Functional MPT Protein as a Therapeutic Agent against <i<Mycobacterium tuberculosis</i< <i<Mycobacterium tuberculosis</i< MPT peptide TBK1 p47phox hexokinase 2 macrophages
topic	misc QH301-705.5 misc <i<Mycobacterium tuberculosis</i< misc MPT peptide misc TBK1 misc p47phox misc hexokinase 2 misc macrophages misc Biology (General)
topic_unstemmed	misc QH301-705.5 misc <i<Mycobacterium tuberculosis</i< misc MPT peptide misc TBK1 misc p47phox misc hexokinase 2 misc macrophages misc Biology (General)
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title	Multi-Functional MPT Protein as a Therapeutic Agent against <i<Mycobacterium tuberculosis</i<
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title_full	Multi-Functional MPT Protein as a Therapeutic Agent against <i<Mycobacterium tuberculosis</i<
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title_sort	multi-functional mpt protein as a therapeutic agent against <i<mycobacterium tuberculosis</i<
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title_auth	Multi-Functional MPT Protein as a Therapeutic Agent against <i<Mycobacterium tuberculosis</i<
abstract	<i<Mycobacterium tuberculosis</i< (MTB), the causative agent of tuberculosis (TB), avoids the host immune system through its virulence factors. MPT63 and MPT64 are the virulence factors secreted by MTB which regulate host proteins for the survival and proliferation of MTB in the host. Here, we found that MPT63 bound directly with TBK1 and p47phox, whereas MPT64 interacted with TBK1 and HK2. We constructed a MPT63/64-derived multifunctional recombinant protein (rMPT) that was able to interact with TBK1, p47phox, or HK2. rMPT was shown to regulate IFN-β levels and increase inflammation and concentration of reactive oxygen species (ROS), while targeting macrophages and killing MTB, both in vitro and in vivo. Furthermore, the identification of the role of rMPT against MTB was achieved via vaccination in a mouse model. Taken together, we here present rMPT, which, by regulating important immune signaling systems, can be considered an effective vaccine or therapeutic agent against MTB.
abstractGer	<i<Mycobacterium tuberculosis</i< (MTB), the causative agent of tuberculosis (TB), avoids the host immune system through its virulence factors. MPT63 and MPT64 are the virulence factors secreted by MTB which regulate host proteins for the survival and proliferation of MTB in the host. Here, we found that MPT63 bound directly with TBK1 and p47phox, whereas MPT64 interacted with TBK1 and HK2. We constructed a MPT63/64-derived multifunctional recombinant protein (rMPT) that was able to interact with TBK1, p47phox, or HK2. rMPT was shown to regulate IFN-β levels and increase inflammation and concentration of reactive oxygen species (ROS), while targeting macrophages and killing MTB, both in vitro and in vivo. Furthermore, the identification of the role of rMPT against MTB was achieved via vaccination in a mouse model. Taken together, we here present rMPT, which, by regulating important immune signaling systems, can be considered an effective vaccine or therapeutic agent against MTB.
abstract_unstemmed	<i<Mycobacterium tuberculosis</i< (MTB), the causative agent of tuberculosis (TB), avoids the host immune system through its virulence factors. MPT63 and MPT64 are the virulence factors secreted by MTB which regulate host proteins for the survival and proliferation of MTB in the host. Here, we found that MPT63 bound directly with TBK1 and p47phox, whereas MPT64 interacted with TBK1 and HK2. We constructed a MPT63/64-derived multifunctional recombinant protein (rMPT) that was able to interact with TBK1, p47phox, or HK2. rMPT was shown to regulate IFN-β levels and increase inflammation and concentration of reactive oxygen species (ROS), while targeting macrophages and killing MTB, both in vitro and in vivo. Furthermore, the identification of the role of rMPT against MTB was achieved via vaccination in a mouse model. Taken together, we here present rMPT, which, by regulating important immune signaling systems, can be considered an effective vaccine or therapeutic agent against MTB.
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container_issue	5, p 545
title_short	Multi-Functional MPT Protein as a Therapeutic Agent against <i<Mycobacterium tuberculosis</i<
url	https://doi.org/10.3390/biomedicines9050545 https://doaj.org/article/81dfed782e084defbedc49002d7a5238 https://www.mdpi.com/2227-9059/9/5/545 https://doaj.org/toc/2227-9059
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up_date	2024-07-03T17:42:59.794Z
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Multi-Functional MPT Protein as a Therapeutic Agent against <i<Mycobacterium tuberculosis</i<

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