Expression Profile of Cell Cycle-Related Genes in Human Fibroblasts Exposed Simultaneously to Radiation and Simulated Microgravity

Multiple unique environmental factors such as space radiation and microgravity (μ<i<G</i<) pose a serious threat to human gene stability during space travel. Recently, we reported that simultaneous exposure of human fibroblasts to simulated μ<i<G</i< and radiation results in...
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Autor*in:	Hiroko Ikeda [verfasserIn] Masafumi Muratani [verfasserIn] Jun Hidema [verfasserIn] Megumi Hada [verfasserIn] Keigi Fujiwara [verfasserIn] Hikaru Souda [verfasserIn] Yukari Yoshida [verfasserIn] Akihisa Takahashi [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2019

Schlagwörter:	simulated microgravity radiation combined effects gene expression cell cycle

Übergeordnetes Werk:	In: International Journal of Molecular Sciences - MDPI AG, 2003, 20(2019), 19, p 4791
Übergeordnetes Werk:	volume:20 ; year:2019 ; number:19, p 4791

Links:	Link aufrufen Link aufrufen Link aufrufen Journal toc

DOI / URN:	10.3390/ijms20194791

Katalog-ID:	DOAJ055981232

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author_variant	h i hi m m mm j h jh m h mh k f kf h s hs y y yy a t at
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allfields	10.3390/ijms20194791 doi (DE-627)DOAJ055981232 (DE-599)DOAJ433eb8e24cd04021b2ce6a85df3c26e4 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Hiroko Ikeda verfasserin aut Expression Profile of Cell Cycle-Related Genes in Human Fibroblasts Exposed Simultaneously to Radiation and Simulated Microgravity 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Multiple unique environmental factors such as space radiation and microgravity (μ<i<G</i<) pose a serious threat to human gene stability during space travel. Recently, we reported that simultaneous exposure of human fibroblasts to simulated μ<i<G</i< and radiation results in more chromosomal aberrations than in cells exposed to radiation alone. However, the mechanisms behind this remain unknown. The purpose of this study was thus to obtain comprehensive data on gene expression using a three-dimensional clinostat synchronized to a carbon (C)-ion or X-ray irradiation system. Human fibroblasts (1BR-hTERT) were maintained under standing or rotating conditions for 3 or 24 h after synchronized C-ion or X-ray irradiation at 1 Gy as part of a total culture time of 2 days. Among 57,773 genes analyzed with RNA sequencing, we focused particularly on the expression of 82 cell cycle-related genes after exposure to the radiation and simulated μ<i<G</i<. The expression of cell cycle-suppressing genes (<i<ABL1</i< and <i<CDKN1A</i<) decreased and that of cell cycle-promoting genes (<i<CCNB1</i<, <i<CCND1</i<, <i<KPNA2</i<, <i<MCM4, MKI67</i<, and <i<STMN1</i<) increased after C-ion irradiation under μ<i<G</i<. The cell may pass through the G<sub<1</sub</S and G<sub<2</sub< checkpoints with DNA damage due to the combined effects of C-ions and μ<i<G</i<, suggesting that increased genomic instability might occur in space. simulated microgravity radiation combined effects gene expression cell cycle Biology (General) Chemistry Masafumi Muratani verfasserin aut Jun Hidema verfasserin aut Megumi Hada verfasserin aut Keigi Fujiwara verfasserin aut Hikaru Souda verfasserin aut Yukari Yoshida verfasserin aut Akihisa Takahashi verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 20(2019), 19, p 4791 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:20 year:2019 number:19, p 4791 https://doi.org/10.3390/ijms20194791 kostenfrei https://doaj.org/article/433eb8e24cd04021b2ce6a85df3c26e4 kostenfrei https://www.mdpi.com/1422-0067/20/19/4791 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2019 19, p 4791
spelling	10.3390/ijms20194791 doi (DE-627)DOAJ055981232 (DE-599)DOAJ433eb8e24cd04021b2ce6a85df3c26e4 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Hiroko Ikeda verfasserin aut Expression Profile of Cell Cycle-Related Genes in Human Fibroblasts Exposed Simultaneously to Radiation and Simulated Microgravity 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Multiple unique environmental factors such as space radiation and microgravity (μ<i<G</i<) pose a serious threat to human gene stability during space travel. Recently, we reported that simultaneous exposure of human fibroblasts to simulated μ<i<G</i< and radiation results in more chromosomal aberrations than in cells exposed to radiation alone. However, the mechanisms behind this remain unknown. The purpose of this study was thus to obtain comprehensive data on gene expression using a three-dimensional clinostat synchronized to a carbon (C)-ion or X-ray irradiation system. Human fibroblasts (1BR-hTERT) were maintained under standing or rotating conditions for 3 or 24 h after synchronized C-ion or X-ray irradiation at 1 Gy as part of a total culture time of 2 days. Among 57,773 genes analyzed with RNA sequencing, we focused particularly on the expression of 82 cell cycle-related genes after exposure to the radiation and simulated μ<i<G</i<. The expression of cell cycle-suppressing genes (<i<ABL1</i< and <i<CDKN1A</i<) decreased and that of cell cycle-promoting genes (<i<CCNB1</i<, <i<CCND1</i<, <i<KPNA2</i<, <i<MCM4, MKI67</i<, and <i<STMN1</i<) increased after C-ion irradiation under μ<i<G</i<. The cell may pass through the G<sub<1</sub</S and G<sub<2</sub< checkpoints with DNA damage due to the combined effects of C-ions and μ<i<G</i<, suggesting that increased genomic instability might occur in space. simulated microgravity radiation combined effects gene expression cell cycle Biology (General) Chemistry Masafumi Muratani verfasserin aut Jun Hidema verfasserin aut Megumi Hada verfasserin aut Keigi Fujiwara verfasserin aut Hikaru Souda verfasserin aut Yukari Yoshida verfasserin aut Akihisa Takahashi verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 20(2019), 19, p 4791 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:20 year:2019 number:19, p 4791 https://doi.org/10.3390/ijms20194791 kostenfrei https://doaj.org/article/433eb8e24cd04021b2ce6a85df3c26e4 kostenfrei https://www.mdpi.com/1422-0067/20/19/4791 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2019 19, p 4791
allfields_unstemmed	10.3390/ijms20194791 doi (DE-627)DOAJ055981232 (DE-599)DOAJ433eb8e24cd04021b2ce6a85df3c26e4 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Hiroko Ikeda verfasserin aut Expression Profile of Cell Cycle-Related Genes in Human Fibroblasts Exposed Simultaneously to Radiation and Simulated Microgravity 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Multiple unique environmental factors such as space radiation and microgravity (μ<i<G</i<) pose a serious threat to human gene stability during space travel. Recently, we reported that simultaneous exposure of human fibroblasts to simulated μ<i<G</i< and radiation results in more chromosomal aberrations than in cells exposed to radiation alone. However, the mechanisms behind this remain unknown. The purpose of this study was thus to obtain comprehensive data on gene expression using a three-dimensional clinostat synchronized to a carbon (C)-ion or X-ray irradiation system. Human fibroblasts (1BR-hTERT) were maintained under standing or rotating conditions for 3 or 24 h after synchronized C-ion or X-ray irradiation at 1 Gy as part of a total culture time of 2 days. Among 57,773 genes analyzed with RNA sequencing, we focused particularly on the expression of 82 cell cycle-related genes after exposure to the radiation and simulated μ<i<G</i<. The expression of cell cycle-suppressing genes (<i<ABL1</i< and <i<CDKN1A</i<) decreased and that of cell cycle-promoting genes (<i<CCNB1</i<, <i<CCND1</i<, <i<KPNA2</i<, <i<MCM4, MKI67</i<, and <i<STMN1</i<) increased after C-ion irradiation under μ<i<G</i<. The cell may pass through the G<sub<1</sub</S and G<sub<2</sub< checkpoints with DNA damage due to the combined effects of C-ions and μ<i<G</i<, suggesting that increased genomic instability might occur in space. simulated microgravity radiation combined effects gene expression cell cycle Biology (General) Chemistry Masafumi Muratani verfasserin aut Jun Hidema verfasserin aut Megumi Hada verfasserin aut Keigi Fujiwara verfasserin aut Hikaru Souda verfasserin aut Yukari Yoshida verfasserin aut Akihisa Takahashi verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 20(2019), 19, p 4791 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:20 year:2019 number:19, p 4791 https://doi.org/10.3390/ijms20194791 kostenfrei https://doaj.org/article/433eb8e24cd04021b2ce6a85df3c26e4 kostenfrei https://www.mdpi.com/1422-0067/20/19/4791 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2019 19, p 4791
allfieldsGer	10.3390/ijms20194791 doi (DE-627)DOAJ055981232 (DE-599)DOAJ433eb8e24cd04021b2ce6a85df3c26e4 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Hiroko Ikeda verfasserin aut Expression Profile of Cell Cycle-Related Genes in Human Fibroblasts Exposed Simultaneously to Radiation and Simulated Microgravity 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Multiple unique environmental factors such as space radiation and microgravity (μ<i<G</i<) pose a serious threat to human gene stability during space travel. Recently, we reported that simultaneous exposure of human fibroblasts to simulated μ<i<G</i< and radiation results in more chromosomal aberrations than in cells exposed to radiation alone. However, the mechanisms behind this remain unknown. The purpose of this study was thus to obtain comprehensive data on gene expression using a three-dimensional clinostat synchronized to a carbon (C)-ion or X-ray irradiation system. Human fibroblasts (1BR-hTERT) were maintained under standing or rotating conditions for 3 or 24 h after synchronized C-ion or X-ray irradiation at 1 Gy as part of a total culture time of 2 days. Among 57,773 genes analyzed with RNA sequencing, we focused particularly on the expression of 82 cell cycle-related genes after exposure to the radiation and simulated μ<i<G</i<. The expression of cell cycle-suppressing genes (<i<ABL1</i< and <i<CDKN1A</i<) decreased and that of cell cycle-promoting genes (<i<CCNB1</i<, <i<CCND1</i<, <i<KPNA2</i<, <i<MCM4, MKI67</i<, and <i<STMN1</i<) increased after C-ion irradiation under μ<i<G</i<. The cell may pass through the G<sub<1</sub</S and G<sub<2</sub< checkpoints with DNA damage due to the combined effects of C-ions and μ<i<G</i<, suggesting that increased genomic instability might occur in space. simulated microgravity radiation combined effects gene expression cell cycle Biology (General) Chemistry Masafumi Muratani verfasserin aut Jun Hidema verfasserin aut Megumi Hada verfasserin aut Keigi Fujiwara verfasserin aut Hikaru Souda verfasserin aut Yukari Yoshida verfasserin aut Akihisa Takahashi verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 20(2019), 19, p 4791 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:20 year:2019 number:19, p 4791 https://doi.org/10.3390/ijms20194791 kostenfrei https://doaj.org/article/433eb8e24cd04021b2ce6a85df3c26e4 kostenfrei https://www.mdpi.com/1422-0067/20/19/4791 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2019 19, p 4791
allfieldsSound	10.3390/ijms20194791 doi (DE-627)DOAJ055981232 (DE-599)DOAJ433eb8e24cd04021b2ce6a85df3c26e4 DE-627 ger DE-627 rakwb eng QH301-705.5 QD1-999 Hiroko Ikeda verfasserin aut Expression Profile of Cell Cycle-Related Genes in Human Fibroblasts Exposed Simultaneously to Radiation and Simulated Microgravity 2019 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Multiple unique environmental factors such as space radiation and microgravity (μ<i<G</i<) pose a serious threat to human gene stability during space travel. Recently, we reported that simultaneous exposure of human fibroblasts to simulated μ<i<G</i< and radiation results in more chromosomal aberrations than in cells exposed to radiation alone. However, the mechanisms behind this remain unknown. The purpose of this study was thus to obtain comprehensive data on gene expression using a three-dimensional clinostat synchronized to a carbon (C)-ion or X-ray irradiation system. Human fibroblasts (1BR-hTERT) were maintained under standing or rotating conditions for 3 or 24 h after synchronized C-ion or X-ray irradiation at 1 Gy as part of a total culture time of 2 days. Among 57,773 genes analyzed with RNA sequencing, we focused particularly on the expression of 82 cell cycle-related genes after exposure to the radiation and simulated μ<i<G</i<. The expression of cell cycle-suppressing genes (<i<ABL1</i< and <i<CDKN1A</i<) decreased and that of cell cycle-promoting genes (<i<CCNB1</i<, <i<CCND1</i<, <i<KPNA2</i<, <i<MCM4, MKI67</i<, and <i<STMN1</i<) increased after C-ion irradiation under μ<i<G</i<. The cell may pass through the G<sub<1</sub</S and G<sub<2</sub< checkpoints with DNA damage due to the combined effects of C-ions and μ<i<G</i<, suggesting that increased genomic instability might occur in space. simulated microgravity radiation combined effects gene expression cell cycle Biology (General) Chemistry Masafumi Muratani verfasserin aut Jun Hidema verfasserin aut Megumi Hada verfasserin aut Keigi Fujiwara verfasserin aut Hikaru Souda verfasserin aut Yukari Yoshida verfasserin aut Akihisa Takahashi verfasserin aut In International Journal of Molecular Sciences MDPI AG, 2003 20(2019), 19, p 4791 (DE-627)316340715 (DE-600)2019364-6 14220067 nnns volume:20 year:2019 number:19, p 4791 https://doi.org/10.3390/ijms20194791 kostenfrei https://doaj.org/article/433eb8e24cd04021b2ce6a85df3c26e4 kostenfrei https://www.mdpi.com/1422-0067/20/19/4791 kostenfrei https://doaj.org/toc/1422-0067 Journal toc kostenfrei GBV_USEFLAG_A SYSFLAG_A GBV_DOAJ GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 20 2019 19, p 4791
language	English
source	In International Journal of Molecular Sciences 20(2019), 19, p 4791 volume:20 year:2019 number:19, p 4791
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topic_title	QH301-705.5 QD1-999 Expression Profile of Cell Cycle-Related Genes in Human Fibroblasts Exposed Simultaneously to Radiation and Simulated Microgravity simulated microgravity radiation combined effects gene expression cell cycle
topic	misc QH301-705.5 misc QD1-999 misc simulated microgravity misc radiation misc combined effects misc gene expression misc cell cycle misc Biology (General) misc Chemistry
topic_unstemmed	misc QH301-705.5 misc QD1-999 misc simulated microgravity misc radiation misc combined effects misc gene expression misc cell cycle misc Biology (General) misc Chemistry
topic_browse	misc QH301-705.5 misc QD1-999 misc simulated microgravity misc radiation misc combined effects misc gene expression misc cell cycle misc Biology (General) misc Chemistry
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title	Expression Profile of Cell Cycle-Related Genes in Human Fibroblasts Exposed Simultaneously to Radiation and Simulated Microgravity
ctrlnum	(DE-627)DOAJ055981232 (DE-599)DOAJ433eb8e24cd04021b2ce6a85df3c26e4
title_full	Expression Profile of Cell Cycle-Related Genes in Human Fibroblasts Exposed Simultaneously to Radiation and Simulated Microgravity
author_sort	Hiroko Ikeda
journal	International Journal of Molecular Sciences
journalStr	International Journal of Molecular Sciences
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lang_code	eng
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author_browse	Hiroko Ikeda Masafumi Muratani Jun Hidema Megumi Hada Keigi Fujiwara Hikaru Souda Yukari Yoshida Akihisa Takahashi
container_volume	20
class	QH301-705.5 QD1-999
format_se	Elektronische Aufsätze
author-letter	Hiroko Ikeda
doi_str_mv	10.3390/ijms20194791
author2-role	verfasserin
title_sort	expression profile of cell cycle-related genes in human fibroblasts exposed simultaneously to radiation and simulated microgravity
callnumber	QH301-705.5
title_auth	Expression Profile of Cell Cycle-Related Genes in Human Fibroblasts Exposed Simultaneously to Radiation and Simulated Microgravity
abstract	Multiple unique environmental factors such as space radiation and microgravity (μ<i<G</i<) pose a serious threat to human gene stability during space travel. Recently, we reported that simultaneous exposure of human fibroblasts to simulated μ<i<G</i< and radiation results in more chromosomal aberrations than in cells exposed to radiation alone. However, the mechanisms behind this remain unknown. The purpose of this study was thus to obtain comprehensive data on gene expression using a three-dimensional clinostat synchronized to a carbon (C)-ion or X-ray irradiation system. Human fibroblasts (1BR-hTERT) were maintained under standing or rotating conditions for 3 or 24 h after synchronized C-ion or X-ray irradiation at 1 Gy as part of a total culture time of 2 days. Among 57,773 genes analyzed with RNA sequencing, we focused particularly on the expression of 82 cell cycle-related genes after exposure to the radiation and simulated μ<i<G</i<. The expression of cell cycle-suppressing genes (<i<ABL1</i< and <i<CDKN1A</i<) decreased and that of cell cycle-promoting genes (<i<CCNB1</i<, <i<CCND1</i<, <i<KPNA2</i<, <i<MCM4, MKI67</i<, and <i<STMN1</i<) increased after C-ion irradiation under μ<i<G</i<. The cell may pass through the G<sub<1</sub</S and G<sub<2</sub< checkpoints with DNA damage due to the combined effects of C-ions and μ<i<G</i<, suggesting that increased genomic instability might occur in space.
abstractGer	Multiple unique environmental factors such as space radiation and microgravity (μ<i<G</i<) pose a serious threat to human gene stability during space travel. Recently, we reported that simultaneous exposure of human fibroblasts to simulated μ<i<G</i< and radiation results in more chromosomal aberrations than in cells exposed to radiation alone. However, the mechanisms behind this remain unknown. The purpose of this study was thus to obtain comprehensive data on gene expression using a three-dimensional clinostat synchronized to a carbon (C)-ion or X-ray irradiation system. Human fibroblasts (1BR-hTERT) were maintained under standing or rotating conditions for 3 or 24 h after synchronized C-ion or X-ray irradiation at 1 Gy as part of a total culture time of 2 days. Among 57,773 genes analyzed with RNA sequencing, we focused particularly on the expression of 82 cell cycle-related genes after exposure to the radiation and simulated μ<i<G</i<. The expression of cell cycle-suppressing genes (<i<ABL1</i< and <i<CDKN1A</i<) decreased and that of cell cycle-promoting genes (<i<CCNB1</i<, <i<CCND1</i<, <i<KPNA2</i<, <i<MCM4, MKI67</i<, and <i<STMN1</i<) increased after C-ion irradiation under μ<i<G</i<. The cell may pass through the G<sub<1</sub</S and G<sub<2</sub< checkpoints with DNA damage due to the combined effects of C-ions and μ<i<G</i<, suggesting that increased genomic instability might occur in space.
abstract_unstemmed	Multiple unique environmental factors such as space radiation and microgravity (μ<i<G</i<) pose a serious threat to human gene stability during space travel. Recently, we reported that simultaneous exposure of human fibroblasts to simulated μ<i<G</i< and radiation results in more chromosomal aberrations than in cells exposed to radiation alone. However, the mechanisms behind this remain unknown. The purpose of this study was thus to obtain comprehensive data on gene expression using a three-dimensional clinostat synchronized to a carbon (C)-ion or X-ray irradiation system. Human fibroblasts (1BR-hTERT) were maintained under standing or rotating conditions for 3 or 24 h after synchronized C-ion or X-ray irradiation at 1 Gy as part of a total culture time of 2 days. Among 57,773 genes analyzed with RNA sequencing, we focused particularly on the expression of 82 cell cycle-related genes after exposure to the radiation and simulated μ<i<G</i<. The expression of cell cycle-suppressing genes (<i<ABL1</i< and <i<CDKN1A</i<) decreased and that of cell cycle-promoting genes (<i<CCNB1</i<, <i<CCND1</i<, <i<KPNA2</i<, <i<MCM4, MKI67</i<, and <i<STMN1</i<) increased after C-ion irradiation under μ<i<G</i<. The cell may pass through the G<sub<1</sub</S and G<sub<2</sub< checkpoints with DNA damage due to the combined effects of C-ions and μ<i<G</i<, suggesting that increased genomic instability might occur in space.
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title_short	Expression Profile of Cell Cycle-Related Genes in Human Fibroblasts Exposed Simultaneously to Radiation and Simulated Microgravity
url	https://doi.org/10.3390/ijms20194791 https://doaj.org/article/433eb8e24cd04021b2ce6a85df3c26e4 https://www.mdpi.com/1422-0067/20/19/4791 https://doaj.org/toc/1422-0067
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author2	Masafumi Muratani Jun Hidema Megumi Hada Keigi Fujiwara Hikaru Souda Yukari Yoshida Akihisa Takahashi
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